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1.
Article in Chinese | WPRIM | ID: wpr-873564

ABSTRACT

Objective To study the effect of metabolic syndrome on the fertility and reproduction in model animals. Methods The model of"high fat diet for spontaneously hypertensive rats(SHR)"was adopted to construct the model of metabolic syndrome in rats. The metabolic syndrome model rats were used to mate with male and female 1 : 1 cage, and the mating cycle was 2 weeks. Results After the SHR rats were fed a high-fat diet for 10 weeks, 16 males and 15 females met the screening criteria for metabolic syndrome, with the modeling rates of 40% and 37.5%, respectively. In addition to the abnormal metabolism-related indicators(such as blood glucose, blood lipid and blood pressure), the male rats with metabolic syndrome mainly had decreased sperm motility(P < 0.05), increased sperm malformation rate(P < 0.01), and decreased mating rate(P < 0.05). In addition to abnormal metabolism-related indicators, the conception rate and the live fetal rate of the female rats with metabolic syndrome were slightly lower than that of the control group; however, there was no statistical difference. The mean birth weight of the litter was significantly lower than that of the control group(P < 0.05). Conclusion According to the whole process from mating to natural production, metabolic syndrome is determined to have a significant effect on the fertility and reproductive ability of rats.

2.
Article in Chinese | WPRIM | ID: wpr-884642

ABSTRACT

Objective:To study the predictive value of the systemic immune inflammation index (SII) on the overall survival rate of patients after Whipple surgery for pancreatic ductal adenocarcinoma (PDAC).Methods:The clinical data of patients with PDAC who underwent Whipple surgery at the First Affiliated Hospital of Zhengzhou University from January 1, 2010 to December 31, 2017 were retrospectively analyzed, and the SII value was calculated. The best cut-off value of SII was 900, and all patients were divided into the low SII group (SII≤900) and the high SII group (SII>900) using 900 as the dividing point. The Kaplan-Meier method was used to draw survival curves and the log-rank test was used. The overall survival of the two groups of patients were analyzed. The Cox risk regression model was used to perform univariate analysis of the various clinicopathological parameters, and multivariate analysis for the statistically significant indicators.Results:Of 135 patients enrolled into this study, there were 78 males and 57 females, aged 28.0-76.0 (56.6±8.8) years. There were 92 patients in the low SII group, aged (56.9±9.2) years with 53 males and 39 females; and 43 patients in the high SII group, aged (56.1±7.9) years, with 25 males and 18 females. The median survival of the low SII group and the high SII group were 32.7 months (95% CI: 28.4-37.0) and 24.4 months (95% CI: 21.4-27.4), respectively. The survival of patients with PDAC in the low SII group was significantly higher than that in the high SII group ( P<0.05). On univariate survival analysis, postoperative overall survival of patients with PDAC was significantly associated with high SII ( HR=2.047, 95% CI: 1.354-3.096), R 1 margin ( HR=2.595, 95% CI: 1.663-4.048), a positive rate of lymph node>20% ( HR=3.244, 95% CI: 1.888-5.573), and positive regional lymph node (N1) ( HR=3.061, 95% CI: 1.993-4.702), all P<0.05. Multivariate Cox regression analysis showed that high SII ( HR=1.672, 95% CI: 1.094-2.555), R 1 resection margin ( HR=2.167, 95% CI: 1.274-3.685), and a positive rate of lymph node>20% ( HR=2.631, 95% CI: 1.309-5.285) to be independent risk factors affecting overall survival ( P<0.05). Conclusion:SII was an independent prognostic factor available before surgery for patients with PDAC. It has some guiding significance in predicting overall survival in patients after Whipple surgery for PDAC.

3.
Article in English | WPRIM | ID: wpr-881081

ABSTRACT

Nonalcoholic fatty liver disease (NAFLD) is regarded as the most common liver disease with no approved therapeutic drug currently. Silymarin, an extract from the seeds of Silybum marianum, has been used for centuries for the treatment of various liver diseases. Although the hepatoprotective effect of silybin against NAFLD is widely accepted, the underlying mechanism and therapeutic target remain unclear. In this study, NAFLD mice caused by methionine-choline deficient (MCD) diet were orally administrated with silybin to explore the possible mechanism and target. To clarify the contribution of peroxisome proliferator-activated receptor α (PPARα), PPARα antagonist GW6471 was co-administrated with silybin to NAFLD mice. Since silybin was proven as a PPARα partial agonist, the combined effect of silybin with PPARα agonist, fenofibrate, was then evaluated in NAFLD mice. Serum and liver samples were collected to analyze the pharmacological efficacy and expression of PPARα and its targets. As expected, silybin significantly protected mice from MCD-induced NAFLD. Furthermore, silybin reduced lipid accumulation via activating PPARα, inducing the expression of liver cytosolic fatty acid-binding protein, carnitine palmitoyltransferase (Cpt)-1a, Cpt-2, medium chain acyl-CoA dehydrogenase and stearoyl-CoA desaturase-1, and suppressing fatty acid synthase and acetyl-CoA carboxylase α. GW6471 abolished the effect of silybin on PPARα signal and hepatoprotective effect against NAFLD. Moreover, as a partial agonist for PPARα, silybin impaired the powerful lipid-lowering effect of fenofibrate when used together. Taken together, silybin protected mice against NAFLD via activating PPARα to diminish lipid accumulation and it is not suggested to simultaneously take silybin and classical PPARα agonists for NAFLD therapy.

4.
Chinese Journal of Lung Cancer ; (12): 305-322, 2021.
Article in Chinese | WPRIM | ID: wpr-880262

ABSTRACT

"The Expert Group on Tumor Ablation Therapy of Chinese Medical Doctor Association, The Tumor Ablation Committee of Chinese College of Interventionalists, The Society of Tumor Ablation Therapy of Chinese Anti-Cancer Association and The Ablation Expert Committee of the Chinese Society of Clinical Oncology" have organized multidisciplinary experts to formulate the consensus for thermal ablation of pulmonary subsolid nodules or ground-glass nodule (GGN). The expert consensus reviews current literatures and provides clinical practices for thermal ablation of GGN. The main contents include: (1) clinical evaluation of GGN, (2) procedures, indications, contraindications, outcomes evaluation and related complications of thermal ablation for GGN and (3) future development directions.
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5.
Article in Chinese | WPRIM | ID: wpr-879194

ABSTRACT

The aim of the research was to evaluate the efficacy and safety associated with Shexiang Tongxin Dropping Pills combined with conventional therapy for patients with coronary heart disease(CHD). We searched 8 electronic databases up to November 2020, including PubMed, Cochrane Library, EMbase, Web of Science, CNKI, Wanfang, VIP and SinoMed. Eligible studies were clinical trials of Shexiang Tongxin Dropping Pills combined with conventional therapy used in the treatment of coronary heart disease(CHD). The Meta-analysis was performed using STATA 15 software. A total of 21 RCTs(n=2 186) were shortlisted for the Meta-analysis. The results of efficacy evaluation showed that the total effective rate of Shexiang Tongxin Dropping Pills combined with conventional therapy was higher than that of conventional therapy of coronary heart disease(RR=1.20, 95%CI[1.15, 1.26], Z=8.63, P<0.001). Furthermore, Shexiang Tongxin Dripping Pills combined with conventional therapy had better effect on electrocardiogram efficacy(RR=1.24, 95%CI[1.16, 1.34], Z=5.98, P<0.001) and the number of angina attacks(SMD=-2.30, 95%CI[-3.47,-1.14], Z=3.88, P<0.001), the duration of angina attack(SMD=-2.31, 95%CI[-3.07,-1.55], Z=5.97, P<0.001), with lower levels of LDL-C(SMD=-0.73, 95%CI[-1.32,-0.14], Z=2.42, P=0.016), TC(SMD=-1.16, 95%CI[-1.35,-0.96], Z=11.56, P<0.001) and TG(SMD=-0.87, 95%CI[-1.06,-0.68], Z=8.97, P<0.001), and higher levels of HDL-C(SMD=0.87, 95%CI[0.02, 1.71], Z=2.00, P=0.045). The results of safety evaluation showed that the incidence of adverse reactions of Shexiang Tongxin Dropping Pills combined with conventional therapy was lower than that of conventional therapy of coronary heart disease(RR=0.45, 95%CI[0.22, 0.91], Z=2.23, P=0.026). There were significant differences in the above outcome indexes between the two groups. After the Harbord method test, the total effective rate outcome index has publication bias, but the sensitivity analysis of the cut-and-fill method suggested that the result was stable. In general, limited by the quantity and quality of included literature, more high-quality studies are needed to further verify the conclusions of this study.


Subject(s)
Angina Pectoris , Coronary Disease/drug therapy , Drugs, Chinese Herbal/adverse effects , Electrocardiography , Humans
6.
Article in Chinese | WPRIM | ID: wpr-879164

ABSTRACT

To evaluate the economics of Suhuang Zhike Capsules in the treatment of acute exacerbation of chronic obstructive pulmonary disease(AECOPD) for inpatients. Based on the published clinical research data, cost-utility analysis was used in this study to evaluate the pharmacoeconomics of Suhuang Zhike Capsules in treatment of AECOPD inpatients from the perspective of medical insu-rance. The test group was treated with Suhuang Zhike Capsules combined with conventional Western medicine, and the control group was treated with conventional Western medicine alone. Treeage software was used to construct a pharmacoeconomic model and perform simulation analysis. The results showed that the cost and output of Suhuang Zhike Capsules combined with the conventional Western medicine were 60 010.18 yuan and 1.92 quality adjusted life year(QALYs), respectively in the simulated 3 years of disease treatment. The cost and output of the conventional Western medicine were 96 730.60 yuan and 1.90 QALYs respectively. Suhuang Zhike Capsules combined with conventional Western medicine required lower cost but achieved higher output, showing cost-utility advantages, so this drug combination was a plan with pharmacoeconomic advantages. The sensitivity analysis results showed that the conclusion was relatively stable. Based on the above results, it is believed that as compared with the conventional Western medicine, Suhuang Zhike Capsules combined with conventional Western medicine have lower cost and higher output for the treatment of AECOPD inpatients, and it is a treatment plan with pharmacoeconomic advantages.


Subject(s)
Capsules , Drugs, Chinese Herbal/therapeutic use , Economics, Pharmaceutical , Humans , Inpatients , Pulmonary Disease, Chronic Obstructive/drug therapy
7.
Article in Chinese | WPRIM | ID: wpr-879098

ABSTRACT

This research was to evaluate the economics of Shexiang Tongxin Dropping Pills combined with conventional therapy for patients with coronary heart disease(CHD) in Chinese medical environment. From the perspective of medical insurance, a Markov model was established in this study based on the results of Meta-analysis comparing the effectiveness and safety of Shexiang Tongxin Dripping Pills combined with conventional treatment and conventional treatment alone. The experimental group was treated with She-xiang Tongxin Dropping Pills combined with conventional Western medicine treatment, while the control group was treated with conventional Western medicine treatment alone. The cost-utility analysis and sensitivity analysis were performed for the two regimens using Treeage pro. After 30 cycles of model simulation, according to the results of Markov model, the total cost and health output were CNY 237 795.73 and 16.36 QALYs(the quality adjusted life years, QALYs), respectively for Shexiang Tongxin Dropping Pills combined with conventional Western medicine treatment, CNY 247 396.55 and 16.36 QALYs respectively for the conventional Western medicine treatment alone. Compared with the conventional treatment alone, the Shexiang Tongxin Dropping Pills combined with conventional treatment had lower long-term cost and higher health output, with advantages of cost-utility and pharmacoeconomic advantages. The sensitivity analysis results showed that the conclusion was relatively stable. Based on the above results, it is considered that compared with the conventional Western medicine alone, Shexiang Tongxin Dropping Pill combined with conventional Western medicine is a treatment regimen with pharmacoeconomic advantages for the treatment of CHD.


Subject(s)
Coronary Disease/drug therapy , Drugs, Chinese Herbal , Economics, Pharmaceutical , Female , Humans
8.
Article in Chinese | WPRIM | ID: wpr-878930

ABSTRACT

The present study is to investigate the absorption characteristics of the main components in Polygonum orientale extract in normal and isoproterenol-induced myocardial ischemia model rats with everted intestinal sac models. Intestinal sac fluid samples were collected in different part of intestine(duodenum, jejunum, ileum, colon) at different time after administration of different concentration of P. orientale extract(5.0,10.0, 20.0 mg·mL~(-1)). An UPLC-TQD method was employed for the determination of six components including orientin, isoorientin, vitexin, protocatechuic acid, kaempferol-3-O-β-D-glucoside and quercitrin in the intestinal sac samples. The absorption rate and cumulative absorption were calculated to analyze the intestinal absorption characteristics of six components in normal and myocardial ischemia model rats. The P-glycoprotein(P-gp) inhibitor was applied to investigate influence of intestinal absorption of six components in P. orientale extract. The results showed that the main absorption sites were concentrated on the duodenum at low concentration, while they were the colon at the medium concentration and the ileum at high concentration in control groups. In the condition of myocardial ischemia model, the main absorption sites focus on the ileum and jejunum at low concentration; the main absorption sites were in the ileum at the medium concentration and main absorption sites were the duodenum and ileum at high concentration. Compared with the normal group, the absorption rate and cumulative absorption of the six components significantly decreased in the model group. P-gp inhibitor markedly increased the absorption rate and cumulative absorption of six components in the model group, inferring that the 6 components may be the substrates of P-gp, and the mechanism needs further study. In this study, it is revealed that the six components of P. orientale extract can be absorbed into the intestinal sac, and it is an effective method to assess the intestinal absorption characteristics of P. orientale extract through everted intestinal sac model, providing data support for the clinical application and further development of P. orientale.


Subject(s)
Animals , Intestinal Absorption , Intestines , Isoproterenol , Myocardial Ischemia/chemically induced , Polygonum , Rats , Rats, Sprague-Dawley
9.
Article in Chinese | WPRIM | ID: wpr-871352

ABSTRACT

Objective:To investigate the effects of β-glucan combined with agonistic anti-CD40 monoclonal antibody (5C11) on the immune functions of dendritic cells (DCs) and the possible molecular mechanism.Methods:Mononuclear cells were separated from fresh concentrated white cells (granulocytes) of healthy subjects using Ficoll density gradient centrifugation, and induced by GM-CSF and IL-4 to differentiate into immature DCs. Following various stimulation (5C11 alone, β-glucan alone, 5C11 combined with β-glucan), flow cytometry was used to detect the expression of CD40, CD80, CD83, CD86 and MHC-Ⅱ molecule HLA-DR on the surface of DCs. ELISA was used to detect the secretion of cytokines including IL-12, IL-6, TNF-α and IL-10. Western blot was used to detect the phosphorylation of proteins related to MAPK signaling pathway.Results:Flow cytometry suggested that β-glucan significantly induced the expression of co-stimulatory molecule CD40 on the surface of DCs ( P<0.05). After the DCs were co-stimulated with β-glucan and 5C11, CD80, CD83 and CD86 expression were further significantly increased, and a strong synergistic effect on CD83 expression, a key marker of DC maturation, was observed ( P<0.01). ELISA showed that β-glucan combined with 5C11 could significantly promote the secretion of cytokines such as IL-12, IL-6 and TNF-α by DCs, and have a synergistic effect on the secretion of IL-12, a critical cytokine in regulating DC functions ( P<0.01). Western blot indicated that the phosphorylation of p38 MAPK and p44/42 MAPK in DCs was increased significantly after combined treatment, and the phosphorylation started earlier and lasted longer compared to that in DCs stimulated with 5C11 or β-glucan alone ( P<0.01). Conclusions:This study suggested that β-glucan combined with 5C11 had a synergistic effect on promoting the maturation and improving the immune functions of DCs, providing a new strategy for the preparation of anti-tumor DC vaccines.

10.
Article in Chinese | WPRIM | ID: wpr-869226

ABSTRACT

Objective:This study aimed to analyze the relationship between cerebral ischemia and cerebral blood flow dynamics (cerebrovascular function score), related physiological, and biochemical indexes.Methods:We selected 366 patients who underwent regular physical examination, cerebrovascular hemodynamics test, and head MRI in the health management center of Shandong Electric Power Central Hospital from May 2016 to April 2019. The patients with cerebral ischemia were selected as the case group (264 cases), and those without cerebral ischemia as the control group (102 cases) to analyze the differences in cerebrovascular function scores, blood pressure, body mass index, and related biochemical indexes between the two groups. The risk factors were also analyzed by multiple logistic regression analysis.Results:The average age of cerebral the ischemia group was (53.0±7.6) years, which consisted of 211 men and 53 women. The average age of the control group was (48.7±7.3) years, which consisted of 87 men and 15 women. The comparison of the mean of continuous variables between the two groups of subjects shows that the mean age [(53.0±7.6) years old vs. (48.7±7.3) years old] and systolic blood pressure [(127.70±17.06) mmHg vs.(122.36±14.75) mmHg] (1 mmHg=0.133 kPa) of cerebral ischemia group were significantly higher than that of control group. The high-density lipoprotein level [(1.29±0.26) mmol/L vs. (1.37±0.30) mmol/L] and cerebrovascular function score [(86.55±18.41) vs. (92.16±10.77)] were significantly lower than those in the control group. The exposure rate of systolic blood pressure ≥140 mmHg and cerebrovascular function score <75 [16.7%(44 cases) vs. 2.9%(3 cases)] in the ischemic group was significantly higher than that in the control group, with statistically significant differences ( P<0.05). Multivariate logistic regression results showed that the variables included in the regression equation of the numeration data model were age and cerebrovascular function score, and the OR (95% CI) were 1.062 (1.020-1.105) and 6.838 (1.583-29.547). Conclusion:Aging, low high-density lipoprotein cholesterol level, and low cerebrovascular function score may be the risk factors affecting MRI-defined cerebral ischemia.

11.
Article in Chinese | WPRIM | ID: wpr-867017

ABSTRACT

Objective:To investigate the effects of maternal fluoxetine exposure on hypothalamic pituitary adrenal(HPA) axis and learning and memory function in female offspring rats.Methods:Pregnant rats were divided into treatment group and control group by a table of random numbers, which were given fluoxetine (10 mg/kg per day) or the same amount of normal saline at 11-20 days of gestation, respectively.Ten female offspring from each group were selected randomly for open field, electrical maze and object recognition tests at 12 weeks after birth, and the concentrations of serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT) were determined.Then they were given an unpredictable chronic stress (UCS) for 21 days, and the previous experiments were repeated.Finally, rats were sacrificed and the hypothalamus tissues were taken to measure the mRNA expression of corticotropin releasing hormone (CRH) and arginine vasopressin (AVP). SPSS 19.0 was used for data describing and t-test. Results:Before UCS, there were no significant differences between the two groups among all the indexes(all P>0.05). After UCS, the horizontal movement and vertical movement of the rats in the two groups were significantly reduced compared with that before treatment, and the horizontal movement of the rats in the treatment group ((37.2±7.2) vs (50.8±8.5), t=4.73, P<0.01) and vertical movement ((10.6±2.0) vs (15.2±5.1), t=2.93, P<0.05) were lower than those in the control group.The correct reaction times decreased and the total reaction time increased in the two groups.While the correct response times of the treatment group were significantly lower than that of the control group (3.4±1.5) vs (4.5±0.9), t=2.36, P<0.05). The object recognition index of the rats in the treatment group decreased significantly compared with the control group ((0.11±0.04) vs (0.16±0.05), t=2.28, P<0.05). Before and after UCS, the change rate of ACTH ((61.13±26.08)% vs (29.83±12.73)%, t=3.67, P<0.01) and CORT ((105.71±18.39)% vs (74.15±39.24)%, t=2.34, P<0.05) in the treated group was significantly higher than that in the control group, and the mRNA expression of CRH in hypothalamus was significantly higher than that in the control group ( t=4.15, P<0.01). Conclusion:No significant damage is found to the HPA axis as well as learning and memory function of female offspring rats after exposure to fluoxetine in pregnancy, but the susceptibility of HPA axis as well as learning and memory function is increased under chronic stress.

12.
Article in Chinese | WPRIM | ID: wpr-798561

ABSTRACT

Objective@#This study aimed to analyze the relationship between cerebral ischemia and cerebral blood flow dynamics (cerebrovascular function score), related physiological, and biochemical indexes.@*Methods@#We selected 366 patients who underwent regular physical examination, cerebrovascular hemodynamics test, and head MRI in the health management center of Shandong Electric Power Central Hospital from May 2016 to April 2019. The patients with cerebral ischemia were selected as the case group (264 cases), and those without cerebral ischemia as the control group (102 cases) to analyze the differences in cerebrovascular function scores, blood pressure, body mass index, and related biochemical indexes between the two groups. The risk factors were also analyzed by multiple logistic regression analysis.@*Results@#The average age of cerebral the ischemia group was (53.0±7.6) years, which consisted of 211 men and 53 women. The average age of the control group was (48.7±7.3) years, which consisted of 87 men and 15 women. The comparison of the mean of continuous variables between the two groups of subjects shows that the mean age [(53.0±7.6) years old vs. (48.7±7.3) years old] and systolic blood pressure [(127.70±17.06) mmHg vs. (122.36±14.75) mmHg] (1 mmHg=0.133 kPa) of cerebral ischemia group were significantly higher than that of control group. The high-density lipoprotein level [(1.29±0.26) mmol/L vs. (1.37±0.30) mmol/L] and cerebrovascular function score [(86.55±18.41) vs. (92.16±10.77)] were significantly lower than those in the control group. The exposure rate of systolic blood pressure ≥140 mmHg and cerebrovascular function score <75 [16.7%(44 cases) vs. 2.9%(3 cases)] in the ischemic group was significantly higher than that in the control group, with statistically significant differences (P<0.05). Multivariate logistic regression results showed that the variables included in the regression equation of the numeration data model were age and cerebrovascular function score, and the OR (95%CI) were 1.062 (1.020-1.105) and 6.838 (1.583-29.547).@*Conclusion@#Aging, low high-density lipoprotein cholesterol level, and low cerebrovascular function score may be the risk factors affecting MRI-defined cerebral ischemia.

13.
Article in Chinese | WPRIM | ID: wpr-793051

ABSTRACT

Based on our experience in treating one patients with non-small cell lung cancer complicated with hyperthyroidism,the following considerations in immunotherapy and pharmaceutical care are proposed:role of iodine contrast and contrast agent selection in patients with hyperthyroidism;selection of hemostatic agents and assessment of thrombosis risk in patients with hemoptysis caused by tumor invasion of bronchus;influence of glucocorticoid use on the treatment with programmed cell death-1(PD-1)inhibitor and the role of PD-1 inhibitors in patients with a history of hyperthyroidism;education methods for patients refuse to receive opioids.The participation of clinical pharmacists in the Multiple Disciplinary Team and the multi-dimensional pharmaceutical monitoring for patients can improve the safety and rationality of medications.

14.
Article in Chinese | WPRIM | ID: wpr-846109

ABSTRACT

Objective: To establish a pharmacokinetic (PK)-pharmacodynamic (PD) model of Periploca forrestii. Methods: The right hindfoot footpad of rats were 0.1 mL complete Freund's Adjuvant (CFA) to establish adjuvant arthritis (AA) rat model. Rats were ig P. forrestii extract (87 g/kg, twice a day for 14 d) and blood were collected via tail vein at 5, 15, 30, 45 min, 1, 1.5, 2, 3, 4, 6, 8, 12, 24 h after the last administration. The concentrations of 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid in plasma samples were detected by HPLC-mass spectrometry (HPLC-MS/MS) to obtain the drug concentration-time curve. The level of interleukin-1β (IL-1β), rheumatoid factor (RF), tumor necrosis factor-α (TNF-α) in plasma samples were determined by kit to obtain the time-effect curve. WinNonLin software was used to fit the PK parameters of P. forrestii, and the time-effect relationship was fitted to obtain the PD parameters. According to the PD parameters, the PK-PD model of P. forrestii was established. Results: The PK-PD model of P. forrestii according to the WinNonLin software was fitted in accordance with the Inhibitory Effect Sigmoid E0 model, in which the blood concentrations of 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid, 5-O-caffeoylquinic acid could be calculated based on the potency values, and the potency values could be calculated based on the blood concentrations. Conclusion: There was a correlation between the concentrations of IL-1β, RF, TNF-α and 3-O-caffeoylquinic acid, 4-O-caffeoylquinic acid and 5-O-caffeoylquinic acid. These three components in P. forrestii extract could inhibit the secretion of IL-1β, RF and TNF-α to treat rheumatiod arthritis.

15.
Article in Chinese | WPRIM | ID: wpr-827995

ABSTRACT

This project is to study the metabolites of Laportea bulbifera extract in rat feces. After the SD rats were gavaged with the extract(136 g·kg~(-1), according to the crude drug dose), the metabolites in their feces were detected by UHPLC-Q-TOF-MS~E technique, and the obtained mass spectrometry data was combined with UNIFI software for prediction. The prototype components and metabolites in rat feces were identified with reference materials and related literature. A total of 43 metabolites were identified(including 8 prototype components and 35 metabolites). The metabolic pathways mainly include monocaffeoylquinic acid(hydrogenation reduction, ring-opening cracking, sulfation, hydroxylation, glucuronidation), quercetin(O-C2 bond ring-opening cleavage, C2-C3 double bond reduction, rutin carbonylation) and so on. The metabolites and metabolic process of L. bulbifera extract in rat feces were clarified, which provided a basis for the study of the active substances and its mechanism of action.


Subject(s)
Administration, Oral , Animals , Chromatography, High Pressure Liquid , Feces , Plant Extracts , Rats , Rats, Sprague-Dawley , Urticaceae
16.
China Pharmacy ; (12): 1891-1896, 2020.
Article in Chinese | WPRIM | ID: wpr-823362

ABSTRACT

OBJECTIVE:To evaluate the benefit and risk of tirofiban in the treatment of acute coronary syndrome (ACS),and to provide evidence-based reference for clinical drug selection and decision. METHODS :Retrieved from domestic and foreign database as PubMed ,the Cochrane Library ,CNKI and Wanfang database ,during the establishment of database to Apr. 2020,two researcher independently screened the literature based on inclusion and exclusion criteria and extracted the data. After the quality evaluation of the included literatures ,based on rapid health technology assessment ,the extracted results were classifiedly evaluated and comprehensively analyzed. RESULTS :A total of 13 researches of systematic review/Meta-analysis and 1 research of pharmacoeconomics were included. Compared with placebo ,tirofiban could significantly reduce all-cause mortality [OR =0.68, 95%CI(0.54,0.86),P=0.000 1] and the incidence of major adverse cardiac events (MACE)in patients with ACS [RR =0.24, 95%CI(0.14,0.40),P<0.01],and increased the incidence of TIMI 3 [OR=5.73,95%CI(2.99.10.97),P<0.01]. Tirofiban and eptifibatide had similar therapeutic efficacy in the treatment of ACS ,but tirofiban significantly increased the risk of TIMI small bleeding in patients with ACS [RR =0.61,95%CI(0.38,0.98),P=0.04]. For ACS patients with non-ST elevation (NSTE-ACS), compared with placbo ,tirofiban significantly reduced the incidence of MACE [RR =0.76,95% CI(0.61,0.96),P=0.018],but significantly increased the risk of bleeding [OR =1.49,95%CI(1.12,1.98),P=0.006],while there was no significant difference in its effects on the all-cause mortality of NSTE-ACS patients (P>0.05). For STEMI patients ,compared with placebo ,tirofiban significantly reduced the all-cause mortality [RR=0.61,95%CI(0.35,1.05),P=0.007] and the incidence of MACE [RR =0.63,95% CI(0.44,0.90),P=0.007]. When combined with liposuction ,tirofiban also significantly reduced the incidence of MACE [RR = 2.05,95%CI(1.71,2.46),P<0.01],and significantly increased the incidence of TIMI 3 [OR=3.18,95% CI(2.4,4.22),P< 0.01],but there was no significant difference in its effects on bleeding risk (P>0.05). The included pharmacoeconomic study showed that patients treated with bivalutine could get 10.07 QALYs,patients treated with heparin combined with tirofiban could get 9.98 QALYs,and the incremental cost-effectiveness ratio bivalutine compared to the latter one was 28 575.77 yuan/QALYs,which was lower than 3 times of the per capita GDP of some cities. CONCLUSIONS :Tirofiban has good efficacy in the treatment of ACS,but it can increase the risk of bleeding than eptifibatide and placebo. Domestic bivalirudin treating for ACS has a cost-effectiveness advantage over tirofiban combined with heparin.

17.
China Pharmacy ; (12): 1562-1568, 2020.
Article in Chinese | WPRIM | ID: wpr-822620

ABSTRACT

OBJECTIVE:To s tudy the intestinal absorption differences of 6 kinds of active constituents of Polygonum orientale (kaempferol,isokaempferol,vitexin,protocatechuic acid ,kaempferol-3-O-β-D-glucoside and quercetin )in normal and myocardial ischemia(MI)model rats. METHODS :UPLC-MS/MS method was adopted to determine the contents of 6 active components in the intestinal circulatory perfusion fluid. Totally male SD 80 rats were divided into normal group and model group ,with 40 rats in each group. Model group was given isoproterenol hydrochloride (50 mg/kg) subcutaneously to induce MI model;normal group was given constant volume of normalsaline, once a day , for consecutive 2 days. 24 h after successful molding ,normal group and model group received in-situ intestinal circulatory perfusion experiment. The effects of different concentration s of P. orientale extract(5.0,10.0, 20.0 mg/mL),different intestinal segments (duodenum,jejunum,ileum,colon),P-glycoprotein(P-gp)inhibitors(verapamil) and bile on the intestinal absorption of each constituent were explored. RESULTS :The linear ranges of concentrations of kaempferol, isokaempferol, vitexin, protocatechuic acid , kaempferol-3-O-β-D-glucoside and quercetin were 3.15-50.40, 3.21-51.31,1.63-52.43,1.60-50.94,1.31-20.97,8.07-129.25 µg/mL(r>0.999). The lower limits of quantification were 7.86, 8.45,6.52,4.00,3.28,16.14 ng/mL,respectively. RSDs of precision ,matrix effect and stability tests were all lower than 11%; the accuracy were 85.64%-107.65%,which were in line with the requirements of biological sample quantification analysis. Except for there was no statistical significance in the absorption of kaempferol absorption in duodenum of model group at different concentrations,absorption of other five constituents in duodenum of normal and model rats increased with the increase of the concentration of active constituents ,and absorption of medium- and/or high- concentration active constituents (except quercetin )in model group was significantly lower than normal group (P<0.05). In normal group ,the absorption of kaempferol was more in jejunum,ileum and colon ,isokaempferol was more in ileum ,vitexin and protocatechuic acid were more in jejunum and ileum , kaempferin-3-O- β-D-glucoside was more in duodenum ,jejunum and colon ,quercetin was more in colon ;in the model group ,the absorption of Polygonum orientale in jejunum and colon was more ,the absorption of isokaempferol in 4 intestinal segments was little different ,vitexin was mainly absorbed in ileum ,protocatechuic acid and kaempferol- 3-O-β-D-glucoside was mainly absorbed in jejunum ,quercetin was mainly absorbed in duodenum and ileum ;in the same intestine ,the absorption of constituents in the model group was less than normal group. After adding verapamil ,absorption of all constituents in the normal group increased ,but the difference was not statistically significant (P>0.05);absorption of kaempferol ,isokaempferol,vitexin,protocatechuic acid and kaempferol- 3-O-β-D-glucoside were all increased significantly in model group (P<0.05),while there was no statistical significance in the increase of quercetin (P>0.05). After the bile flowed into the duodenum ,absorption of protocatechuic acid was increased significantly in normal group (P<0.05);absorption of other active constituents were increased significantly in model group,except for isokaempferol and quercetin (P<0.05). CONCLUSIONS :Six active constituents of P. orientale were absorbed in the whole intestine of normal and MI model rats ,and the absorption of above constituents may be enhanced more significantly by P-gp inhibitor and bile under pathological condition.

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China Pharmacy ; (12): 1458-1451, 2020.
Article in Chinese | WPRIM | ID: wpr-822365

ABSTRACT

OBJECTIVE:To provide reference for improving the quality standard of Rhizoma Bego niae from Guizhou . METHODS:Five batches of Rhizoma Begoniae from Guizhou were collected ,and the microscopic characteri stics of the Rhizoma Begoniae powder were observed. According to the corresponding methods in 2015 edition of Chinese Pharmacopoeia (part Ⅳ), potent adenosine 50-triphosphate competitive phosphati - dylinositol-3-kinase/mammalian target of rapamycin inhibitors:discovery of compound 26(PKI-587),a highly effi cacious dual inhibitor Morpholine as a privileged structure :a review on the me - dicinal chemistry and pharmacological activity of morpho - line containing bioactive molecules[J]. Med Res Rev , qq.com 2019. DOI :10.1002/med.21634. qualitative identification of Rhizoma Begoniae was conducted by TLC ,and the contents of moisture ,total ash and water-soluble extract in Rhizoma Begoniae were determined. The contents of rutin were determined by HPLC. RESULTS :The powder of Rhizoma Begoniae medicinal materials was brown ,stone cells were square ,polygonal-like or irregular. There were many starch grains and few complex grains. The conduit ,calcium oxalate square crystal/cluster crystal were visible. The same fluorescence spots were found in the same location of TLC atlas of Rhizoma Begoniae control herb. The moisture ,total ash ,water-soluble extract contents were 10.15%-11.41%,8.70%-12.59% and 16.91%-19.58%,respectively. The linear range of rutin were 18.47-147.8 μg/mL (r=0.999 8);RSDs of reproducibility ,intermediate precision and stability tests (8 h)were all lower than 3.0%;the average recoveries were 99.39%-100.29%(RSDs were 0.23%-2.59%,n=3);the contents of rutin in 5 batches of Rhizoma Begoniae were 0.102%-0.198%. CONCLUSIONS :The contents of moisture and total ash shall not exceed 13.0% and 14.0% respectively, and the contents of water-soluble extract and rutin shall not be less than 15.0% and 0.080%. The quality standard established in this study can be used for the quality control of Rhizoma Begoniae from Guizhou.

19.
Article in Chinese | WPRIM | ID: wpr-821657

ABSTRACT

Objective To examine the effect of rational emotive therapy on negative emotions among advanced schistosomiasis patients with repeated hospitalizations. Methods A total of 97 advanced schistosomiasis patients with anxiety and depressive emotions that were hospitalized in Xiangyue Hospital of Hunan Institute of Schistosomiasis Control for three times or more were enrolled, and given rational emotive therapy for 4 weeks in addition to routine nursing care. The scores for anxiety, depression and quality of life were estimated in patients before and after the rational emotive therapy using the Self-Rating Anxiety Scale (SRS), the Self-Rating Depression Scale (SDS) and WHOQOL-BREF Form. Results The SAS and SDS scores were significantly lower 4 weeks following rational emotive therapy than before the intervention (SAS score, 45.40 ± 7.77 vs. 59.25 ± 9.29, t = 14.021, P < 0.01; 51.48 ± 8.01 vs. 63.93 ± 9.59, t = 12.991, P < 0.01). The percentages of patients with moderate and severe anxiety and depression were significantly lower 4 weeks following rational emotive therapy than before the intervention (P < 0.01), and the scores for each item in the quality of life were all significantly greater 4 weeks following rational emotive therapy than before the intervention (P < 0.01). Conclusion Rational emotive therapy may improve the negative emotions and the quality of life of advanced schistosomiasis patients with repeated hospitalizations.

20.
Article in Chinese | WPRIM | ID: wpr-772035

ABSTRACT

OBJECTIVE@#To determine the ratio of deficient mismatch repair (dMMR) proteins and Lynch syndrome among patients undergoing colorectal cancer resection.@*METHODS@#From June 2014 to May 2016, immunohistochemistry for mismatch repair proteins including mutL homolog 1 (MLH1), mutS homolog 2 (MSH2), mutS homolog 6 (MSH6) and PMS1 homolog 2 (PMS2) were carried out on 207 surgically resected specimens. Samples with lost expression of MMR proteins underwent genetic testing.@*RESULTS@#Loss of expression of MMR proteins were found among 21 patients and accounted for 10.14% of the colorectal cancers. dMMR was more common in patients ≤50 years old, or with proximal tumor at splenic flexure and mucinous adenocarcinoma. Ten patients underwent genetic testing, with three pathogenic mutations (MSH6 c.3013C>T, MLH1 c.199G>A and a novel MSH6 c.584delT) and four ambiguous mutations identified. At least 1.4% of the colorectal cancers were diagnosed as Lynch syndrome.@*CONCLUSION@#Routine screening for Lynch syndrome among patients with colorectal cancer with MMR protein immunohistochemistry as preliminary screening method and MMR gene sequencing as diagnostic method is effective and feasible. It can reduce missed diagnosis of Lynch syndrome and bring lifelong benefit to patients and their families.


Subject(s)
Adolescent , Colorectal Neoplasms, Hereditary Nonpolyposis , Early Detection of Cancer , Humans , Immunohistochemistry , Middle Aged , Mismatch Repair Endonuclease PMS2 , MutS Homolog 2 Protein
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