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1.
Article in Chinese | WPRIM | ID: wpr-887940

ABSTRACT

The cross combination of dry-method(network pharmacology analysis) and wet-method(high-resolution mass spectro-metry with antioxidation experiment) was used to predict antioxidant quality markers(Q-markers) of Hippophae tibetana. Ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry(UPLC-Q-Exactive Orbitrap-MS) was developed to rapidly separate and identify the chemical constituents in H. tibetana. Then in DPPH free radicals and superoxide anion scavenging experiment, the antioxidant activity of the four different polar parts with extracts of petroleumether, ethyl acetate, n-butanol and water was evaluated. Network pharmacology method was used for functional enrichment and pathway analysis to screen antioxidant-related components and preliminarily explain the mechanism of action. On this basis, multi-source information was integrated to predict the antioxidant Q-markers. The results showed that 51 components in H. tibetana were identified, including 18 flavonoids, 14 terpenoids, 6 alkaloids, 4 coumarins and phenylpropanoids, 3 volatile components and 2 polyphenols. The antioxidant capacity of different fractions: ethyl acetate > n-butanol > water > petroleum ether. The medicine mainly acted on PI3 K-Akt and FoxO signaling pathways to perform antioxidant effects through flavonoids such as quercetin, luteolin and kaempferol. According to the results of dry-method and wet-method, quercetin, luteolin and kaempferol, the representatives of poly-hydroxy flavone, may be the antioxidant Q-markers of H. tibetana. In this study, with the antioxidant Q-markers of H. tibetana as an example, an investigation model of predicting Q-marker was discussed based on the ternary system of composition, function and informatics, providing a scientific basis for the establishment of quality evaluation standards for H. tibetana.


Subject(s)
Antioxidants , Chromatography, High Pressure Liquid , Hippophae , Mass Spectrometry , Technology
2.
Article in Chinese | WPRIM | ID: wpr-512940

ABSTRACT

Objective To observe the symptom and characteristics of liver injury induced by different dosage of long-term administration of rifampicin(RIF) in mice.Methods Twenty-four healthy female ICR mice were randomly divided into 4 groups(6 mice in each group):control group,low dosage group,medium dosage group and high dosage group.The four groups were treated with 0,100,200,400 mg·kg-1·d-1 RIF respectively for 2 weeks.Mice blood and liver tissue samples were collected at 6 hours after the last administration for serological test and liver histological observation.Results No mice died before execution.The TBA,DBIL and TBIL of high dosage group all increased compared with the control group, but the ALT,AST and ALP showed no obvious change.The TBA and DBIL of medium dosage group increased compared with the control group, while the TBIL,ALT,AST and ALP showed no obvious change.In the low dosage group,there was no obvious change in terms of TBA,DBIL,TBIL,ALT,AST,and ALP compared with the control group.Obvious pathological change occured in the liver of mice in all the experimental groups.HE staining showed edema and feather steatosis in liver cells, accompanied by a large number of inflammatory cells infiltration and a few sporadic cholestasis.With the increasing of RIF dosage,the liver pathological change became more obviously.In the experimental group,electron microscope showed that there were a lot of fat droplets in the liver cells wrapped slurry,and part of the capillary bile duct were slightly expanded with different electron density and irregular shape of bile sample material inside.The pathologic changes get more obvious with the increase of the concentration of rifampicin as well.Conclusion RIF could induce liver injury after 2 weeks' treatment at different dosages,mainly pathological changes included liver cell steatosis,inflammatory cell infiltration,and cholestasis.Rifampicin induced liver injury in a concentration-dependent manner.However,the mechanism of rifampicin-induced liver injury in mice needs further study.

3.
Article in English | WPRIM | ID: wpr-8203

ABSTRACT

PURPOSE: To demonstrate the feasibility, safety, and technical strategies of hand-assisted laparoscopic complete mesocolic excision (HAL-CME) and to compare oncological outcomes between HAL-CME and the open approach (O-CME) for right colon cancers. METHODS: Patients who were scheduled to undergo a right hemicolectomy were divided into HAL-CME and O-CME groups. Measured outcomes included demographic variables, perioperative parameters, and follow-up data. Demographic variables included age, sex distribution, body mass index (BMI), American Society of Anesthesiologists (ASA) physical status classification, previous abdominal surgery, tumor localization, and potential comorbidities. Perioperative parameters included incision length, operative time, blood loss, conversion rate, postoperative pain score, postoperative first passage of flatus, duration of hospital stay, total cost, number of lymph nodes retrieved, TNM classification, and postoperative complications. Follow-up data included follow-up time, use of chemotherapy, local recurrence rate, distant metastasis rate, and short-term survival rate. RESULTS: In total, 150 patients (HAL-CME, 78; O-CME, 72) were included. The groups were similar in age, sex distribution, BMI, ASA classification, history of previous abdominal surgeries, tumor localization, and potential comorbidities. Patients in the HAL-CME group had shorter incision lengths, longer operative times, less operative blood loss, lower pain scores, earlier first passage of flatus, shorter hospital stay, higher total costs, similar numbers of lymph nodes retrieved, similar TNM classifications, and a comparable incidence of postoperative complications. The 2 groups were also similar in local recurrence rate, distant metastasis rate, and short-term survival rate. CONCLUSION: The results demonstrate that the HAL-CME procedure is a safe, valid, and feasible surgical method for right hemicolon cancers.


Subject(s)
Body Mass Index , Classification , Colectomy , Colonic Neoplasms , Comorbidity , Drug Therapy , Flatulence , Follow-Up Studies , Hand-Assisted Laparoscopy , Humans , Incidence , Laparoscopy , Length of Stay , Lymph Node Excision , Lymph Nodes , Mesocolon , Methods , Neoplasm Metastasis , Operative Time , Pain, Postoperative , Postoperative Complications , Recurrence , Sex Distribution , Survival Rate
4.
Article in Chinese | WPRIM | ID: wpr-604837

ABSTRACT

Objective To investigate the influence of transcriptional factor Sp1 on expression of bile acids transporters MRP3 and MRP4 so as to perfect the regulatory mechanism of MRP3 and MRP4 expression.Methods Transformed Sp1-overexpression and Sp1 siRNA plas-mids to HepG2 cell and obtained the stably cells line.Then the expression levels of bile acids transporters MRP3 and MRP4 were measured by RT-qPCR,and the change of protein levels were detected by Western blot.Results The stably cells line Sp1-OE-HepG2 and Sp1siRNA-HepG2 were successfully transformed.The mRNA expression and protein levels of MRP3 and MRP4 were significantly increased in Sp1-OE-HepG2 cells,among which the mRNA expression of MRP3 mRNA increased 2.8 times,the protein levels of MRP3 increased 3.0 times,and the mRNA expression and protein levels of MRP4 increased 3.2 times and 2.5 times respectively.Conversely,the mRNA expression and protein levels of MRP3 and MRP4 were decreased in Sp1 siRNA-HepG2 cells,among which the mRNA expression of MRP3 mRNA de-creased 52%,the mRNA expression of MRP4 mRNA decreased 58%,the protein levels of MRP3 decreased 57%,and the protein levels of MRP4 decreased 60%.Conclusion Transcriptional factor Sp1 could regulate the expression of bile acids transporters MRP3 and MRP4 in HepG2 cells.

5.
Article in Chinese | WPRIM | ID: wpr-500088

ABSTRACT

Objective To study the function and effect of Oleanolic acid to cholestasis on regulating bile acids transporters. Methods A total of 45 rats were divided into 9 groups,5 rats in each group. The Sham group,Bile duct ligation group and Oleanolic acid group were treated with saline or Oleanolic acid for 3 days,7 days and 14 days. Then the liver and tested bile acids transporters in mRNA and protein lev-els were collected. Results The expression of bile acids transporter OSTβ with Oleanolic acid was increased after 3 days. The expression of OSTβ and BESP in Oleanolic acid group after 7 days were increased than those in Sham group. Fourteen days later,the increasing tendency of OSTα,OSTβ and BSEP were sensiable. Conclusion Oleanolic acid can stimulates the expression of bile acids transporters OSTalpha-beta and BSEP in bile duct-ligation rats.

6.
Article in Chinese | WPRIM | ID: wpr-490506

ABSTRACT

Objective To analyze the computed tomography (CT) and magnetic resonance imaging (MRI) features of intestinal-type and pancreaticobiliary-type invasive adenocarcinomas of the ampullary region (IAARs) and investigate the value of the differential diagnosis.Methods The retrospective cross-sectional study was adopted.The clinicopathological data of 33 patients with IAAR who were admitted to the Lanzhou University Second Hospital (20 patients) and Affiliated Hospital of Ningbo University (13 patients) between September 2013 and August 2015 were collected.The patients underwent plain and enhanced scans of CT and MRI.(1) Observation indictors included tumor size,shape,growth pattern,boundary,internal structure,density and/or signal,style and/or degree of contrast enhancement,changes of bile and/or pancreatic duct,invasion and metastasis.(2) The clinical and imaging features of intestinal-type and pancreaticobiliary-type IAARs were compared.(3) Operation was performed after preoperative examinations,and patients received the postoperative pathological examination and immunohistochemistry.Measurement data with normal distribution were presented as x-±s.Comparison between groups was evaluated with an independent sample t test,and count data were analyzed using the chi-square test.Results (1) Of 33 patients with IAAR,19 received plain scan of CT,including 17 receiving simultaneous enhanced scan of CT.Fourteen patients [9 receiving diffussion-weighted imaging (DWI) sequence of MRI] received plain scan of MRI,including 12 receiving simultaneous enhanced scan of MRI.The maximum diameter of IAAR in 33 patients was (2.8 ± 1.4)cm.Fourteen tumors were round-like or oval shape and 19 tumors were irregular shape.The intracavity type,extracavity type and mixed type of tumors were detected in 24,6 and 3 patients,respectively.There were clear boundary of tumors in 17 patients and fuzzy boundary of tumors in 16 patients.Tumors of 5 patients had appeared necrotic and/or cystic,no hemorrhage or calcification was found in tumors of 33 patients.Density and signal of tumors were homogeneous in 18 patients and inhomogeneous in 15 patients.Of 29 patients receiving enhanced scan of CT or MRI,homogeneous enhancement and inhomogeneous enhancement were respectively detected in 14 and 15 patients,and mild enhancement,moderate enhancement and no enhancement were respectively detected in 15,14 and 0 patients.Tumors of 9 patients in DWI showed slightly high or high signal.Thirty patients had secondary bile duct dilatation (3 with mild dilatation,6 with moderate dilatation and 21 with severe dilatation),and 3 patients had no changes of bile duct.Twenty-six patients had secondary pancreatic duct dilatation and 7 had no changes of pancreatic duct.Sixteen patients had tumor invasion to pancreatic tissues and 7 had lymph node metastases.(2) Of 33 patients with IAAR,19 had intestinal-type IAAR (8 males and 11 females) and 14 had pancreaticobiliary-type IAAR (11 males and 3 females).There was statistically significant difference in the gender between the 2 types of IAAR (x2=4.388,P < 0.05).The intracavity type,extracavity type and mixed type of tumors,clear boundary and fuzzy boundary of tumors,homogeneous and inhomogeneous density and/or signal of tumors,with and without tumor invasion to pancreatic tissues were respectively detected in 17,0,2,13,6,14,5,6,13 patients with intestinal-type IAAR and 7,6,1,4,10,4,10,10,4 patients with pancreaticobiliary-type IAAR,with statistically significant differences between the 2 types of IAAR (x2=9.971,5.125,6.617,5.125,P < 0.05).(3) All the patients underwent surgery and received comprehensive diagnosis and tumor classification after pathological examination and immunohistochemistry.Conclusion There are certain characteristics of CT and MRI in intestinal-type and pancreaticobiliary-type IAARs,and gender,tumor growth pattern,boundary,density and/or signal,tumor invasion to pancreatic tissues have certain reference values for the differential diagnosis of intestinal-type and pancreaticobiliary-type IAARs.

7.
Article in Chinese | WPRIM | ID: wpr-473259

ABSTRACT

ObjectiveTo investigate the value of 64-slice CT urography (CTU) in diagnosis of urologic diseases.Methods Sixty patients with suspected urinary system diseases underwent 64-slice CT volume scanning and 3D reconstructions.The diagnostic value of 64-slice CTU was evaluated.ResultsThere were 14 patients with urinary system cancers,2 with ureter polyp,9 with tuberculosis of urinary system,13 with urinary system calculus,13 with nonmalignant ureterostenosis combined with hydronephrosis,2 with ureterostenosis and hydronephrosis induced by nearby organic damage,2 with traumatism,1 with renal occlusion,as well as 4 after urinary tract surgeries and 2 without abnormality.Totally 28 cases was confirmed with surgery and pathology,the sensitivity,specificity and accuracy of MSCTU for upper tract tumor was 100%(14/14),85.71% (12/14) and 92.86% (26/28),respectively.ConclusionMSCTU is able to directly and clearly show urologic diseases.It can be regarded as the first choice in patients with clinically suspected urinary tract diseases,especially tumors.

8.
Article in Korean | WPRIM | ID: wpr-66565

ABSTRACT

It has been decades since the concept of empty follicle syndrome (EFS), in which no oocytes are retrieved in an in vitro fertilization and embryo transfer (IVF-ET) cycle, was introduced. The incidence of this syndrome in patients undergoing IVF-ET has been estimated to be about 0.6-7%. The use of recombinant human chorionic gonadotropin (r-hCG) can be a useful option for the EFS patients with previous failure of oocyte retrieval in IVF-ET cycles. We present a case of empty follicle syndrome with successful oocyte retrieval by the use of r-hCG.


Subject(s)
Chorionic Gonadotropin , Embryo Transfer , Fertilization in Vitro , Humans , Incidence , Oocyte Retrieval , Oocytes
9.
Article in Chinese | WPRIM | ID: wpr-546902

ABSTRACT

Objective To evaluate the diagnostic value of MRI in hepatic echinococcosis.Methods MRI features of 37 cases with hepatic echinococcosis proved by operation and pathology were analysed in comparison with that of CT.Results The hepatic echinococcosis could be divided into five types according to the manifestations of CT and MRI:simple cyst in 8 cases,multiple daughter cyst in 13 cases,ruptured cyst in 7 cases,consolidated and calcified cyst in 4 cases and mixed cyst in 5 cases.Conclusion MRI can clearly show and determine the location,size,form,number and types of hepatic echinococcosis,that are helpful for the plan of operation.

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