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1.
China Pharmacy ; (12): 38-45, 2022.
Article in Chinese | WPRIM | ID: wpr-907010

ABSTRACT

OBJECTIVE To study the absorbed components of Xiebai powder in blood. METHODS UPLC-Q-TOF-MS/MS method was adopted. SD rats were randomly divided into blank group and administration group ,with 10 rats in each group. Blank group was given water intragastrically ,and administration groups were given 2 g/mL(by the amount of crude drug )Xiebai powder solution intragastrically. Administration volume was 11.3 mL/kg,twice a day for 3 days. One point five hours after last administration,blood was taken from the abdominal aorta of each rat ,the serum was processed to obtain the supernatant for analysis;the relevant data in positive and negative ion mode were collected ,and the absorbed components of Xiebai powder in blood were analyzed and identified by using self-built secondary mass spectrometry database and consulting the relevant literature. RESULTS Totally 17 components from Xiebai powder were identified ,among which 6 components came from sovereign Moru salba,7 from minister Cortex Lycii ,12 from assistant Glycyrrhiza uralensis ,i.e. kukoamine A ,chlorogenic acid ,tachiogroside B,astringin,neoglycyrrhizin,glycyrrhizin,azelaic acid ,isoglycyrrhizin,glycyroside,anthocyanin,sebacic acid ,parthenolide, anthocyanin,18β-glycyrrhetinic acid ,6-gingerol,palmitoamide,erucamide. These compounds were mainly flavonoids ,alkaloids and organic acids. CONCLUSIONS In this study ,17 absorbed components of Xiebai powder in blood are preliminarily determined,which are consistent with the effect of Xiebai powder. They may be the pharmacodynamic substances of Xiebai powder.

2.
Chinese Journal of Radiology ; (12): 615-620, 2021.
Article in Chinese | WPRIM | ID: wpr-884455

ABSTRACT

Objective:To explore the value of synthetic MRI in quantitative monitoring of knee joint structural and cartilage changes of amateur marathon runners before and after the whole marathon.Methods:Totally 26 amateur marathon enthusiasts from Zhuhai City, Guangdong Province were recruited from October 2019 to January 2020. The right knee joints were scanned 1 week before the race and within 48 h after the race. The scanning sequence included the three-dimensional proton density weighted image with isotropic (3D-CUBE-PD) sequence and synthetic MRI sequence. The conventional contrast weighted images T 1WI, T 2WI, proton density (PD) weighted imaging, short-T 1 inversion recovery (STIR) and T 1, T 2, PD mapping were obtained by the latter scans. The 3D-CUBE-PD sequence was used as a reference to evaluate the detection of knee joint lesions. The knee articular cartilage was divided into 8 subregions: central medial femoral condyle (CMFC), posterior medial femoral condyle (PMFC), central lateral femoral condyle (CLFC), posterior lateral femoral condyle (PLFC), medial tibia plateau (MTP), lateral tibia plateau (LTP), patella and trochlear. Based on the synthetic MRI quantitative mapping, the T 1, T 2 and PD values of each cartilage subregion were measured independently by 2 radiologists. The ICC was used to evaluate the consistency of the measurement between observers. The T 1, T 2 and PD values of knee cartilage before and after marathon exercise were compared by Wilcoxon signed rank test. Results:The 2 radiologists had good consistency in the measurement of T 1, T 2 and PD values of knee articular cartilage with the ICC values of 0.912, 0.933 and 0.954, respectively. The synthetic MRI quantitative mapping sequence can detect all cartilage damage ( n=3) and joint effusion ( n=15), and 7 of 9 meniscus injuries were detected. The T 1, T 2 and PD values of the knee cartilage as a whole before the race were higher than those after race, and the differences were statistically significant (all P<0.05). The T 1 values were statistically significant except patellar cartilage and trochlear cartilage, and T 2 values were significantly different in the CMFC, LTP, MTP ( P<0.05). Conclusion:Synthetic MRI has a good display of knee joint structural lesions, and its quantitative parameters T 1, T 2 and PD can detect the changes of knee cartilage before and after marathon.

3.
Article in Chinese | WPRIM | ID: wpr-883443

ABSTRACT

Objective:To explore the risk factors of recurrence after laparoscopic myomectomy and provide references for clinical prevention and treatment.Methods:The clinical data of 216 patients who underwent laparoscopic myomectomy in Ningjin County People′s Hospital from June 2016 to December 2018 were analyzed retrospectively. The recurrence rate at 12 months after the operation was followed up, and the risk factors influencing the recurrence after laparoscopic myomectomy were screened by univariate analysis and multivariate Logistic regression model.Results:After followed up for 12 months after the operation, 24 cases had recurrence, with a recurrence rate of 11.11%(24/216). Univariate analysis showed that the recurrence after laparoscopic myomectomy was significantly correlated with surgical age, age at menarche, number of fibroids, uterine size, and gonadrotropin releasing hormone agonist (GnRH-a) treatment after the operation ( P<0.05), but was not significantly correlated with body mass index, fibroid size, fibroid type, and pregnancy after the operation ( P>0.05). Multivariate Logistic regression analysis showed that surgical age ≥ 35 years old ( OR=1.289, 95% CI 1.013-1.641), age at menarche <13 years old ( OR=1.765, 95% CI 1.167 - 2.669), and number of fibroids ≥ 2 ( OR=2.487, 95% CI 1.442 - 4.288) were independent risk factors for recurrence after laparoscopic myomectomy ( P<0.05), while GnRH-a treatment after the operation ( OR = 0.696, 95% CI 0.510-0.951) was its protective factor ( P<0.05). Receiver operating characteristic (ROC) curve analysis showed that the area under the curve of surgical age ≥ 35 years old, menarche age <13 years old, and number of fibroids ≥ 2 for predicting recurrence after laparoscopic myomectomy was 0.641 (95% CI 0.573 - 0.705), 0.719 (95% CI 0.654 - 0.778) and 0.622 (95% CI 0.554 - 0.687), and age at menarche had the greatest diagnostic efficiency. Conclusions:Surgical age ≥ 35 years old, age at menarche <13 years old, and number of fibroids ≥ 2 are independent risk factors for recurrence after laparoscopic myomectomy. Intraoperative ultrasound and postoperative GnRH-a treatment can help reduce the risk of postoperative recurrence.

4.
Journal of Clinical Hepatology ; (12): 2191-2197, 2021.
Article in Chinese | WPRIM | ID: wpr-904868

ABSTRACT

Protein ubiquitination is widely observed in cells and is a modification after protein translation. Hepatitis B virus (HBV) and ubiquitination of related proteins have attracted more and more attention. This article reviews HBV and the ubiquitination of related proteins, so as to provide a reference for further research on the regulation of HBV replication and the ubiquitination of related proteins, as well as new ideas and methods for curing chronic HBV infection.

5.
Journal of Chinese Physician ; (12): 996-1000, 2021.
Article in Chinese | WPRIM | ID: wpr-909655

ABSTRACT

Objective:To analyze the predictive value of D-dimer combined with activated partial thromboplastin time (APTT) for slow / no reflow in patients with acute coronary syndrome (ACS) during percutaneous coronary intervention(PCI).Methods:From June 2017 to June 2019, 316 cases of patients with ACS who were to undergo PCI in Guigang People′s Hospital were selected as the study objects. The patients were divided into slow / no reflow group (SNR) and normal blood flow group (CON) according to the blood flow grading of thrombolysis in myocardial infarction (TIMI) during the operation. The differences of D-dimer and APTT between the two groups before operation were compared, and the predictive value of D-dimer combined with APTT for SNR was analyzed.Results:According to TIMI blood flow grading, all the patients were divided into SNR group ( n=71, 22.47%) and CON group ( n=245, 77.53%). The level of D-dimer in SNR group was significantly higher than that in CON group, while APTT was significantly lower than that in CON group ( P<0.05). Logistic regression analysis showed that D-dimer elevation ( OR=1.011, 95% CI: 1.008-1.015, P<0.001) was an independent risk factor of slow / no reflow in PCI for ACS patients, while APTT elevation ( OR=0.868, 95% CI: 0.818-0.921, P<0.001) was a protective factor. The D-dimer and APTT predicted that the area under receiver operating characteristic (ROC) curve of slow / no reflow in PCI were 0.814 and 0.738 respectively. The area under ROC curve of combined detection of D-dimer and APTT increased to 0.869, and the sensitivity and specificity were 87.3% and 75.1% respectively ( P<0.001). Conclusions:The D-dimer and APTT have a high predictive value of slow / no reflow during PCI in ACS patients. The combined detection of the two is helpful to identify the occurrence of slow / no reflow in PCI.

6.
Article in Chinese | WPRIM | ID: wpr-906523

ABSTRACT

Objective:To study the effect of sedimentary type Limonitum on hemostatic indexes in blood and metal ions in serum of rats with hemorrhage. Method:The hemorrhagic rat models were established by warfarin sodium. The experimental animals were divided into control group,model group,powder group and water decoction group. On day 15 from drug administration, the contents of 6-keto prostaglandin F<sub>1</sub><italic><sub>α</sub></italic>(6-keto-PGF<sub>1</sub><italic><sub>α</sub></italic>),thromboxane B<sub>2</sub>(TXB<sub>2</sub>),arachidonic acid(AA),endothelin 1(ET-1),platelet activating factor(PAF),P-selectin(PS),and Ca<sup>2+</sup> in the whole blood of rats in each group were determined by enzyme-linked immunosorbent assay(ELISA). The contents of Na,Mg,K,Ca,Fe,Al,Li,Be,Ti,V,Cr,Mn,Co,Ni,Cu,Zn,As,Sr,Cd,Sn,Sb,Ba,and Pb in serum samples were determined by inductively coupled plasma optical emission spectrometer(ICP-OES) and inductively coupled plasma mass spectrometry(ICP-MS). Result:Compared with the model group,the content of 6-keto-PGF<sub>1</sub><italic><sub>α</sub></italic> was reduced in the powder group and water decoction group (<italic>P</italic><0.05),and the contents of TXB<sub>2</sub>,AA,ET-1,PAF,PS,Ca<sup>2+ </sup>were<sup> </sup>significantly increased(<italic>P</italic><0.01),with a positive and beneficial regulatory effect. In the powder group, 10 kinds of metal elements in serum of rats were significantly and positively regulated: Na,K,Ca,Fe,Li,Ti,V,Co,Cu,and Zn(<italic>P</italic><0.05,<italic>P</italic><0.01). In the water decoction group, 10 metal elements with significant positive regulation were as follows: Na,K,Ca,Fe,Li,V,Ni,Cu,Zn,and Sr(<italic>P</italic><0.05,<italic>P</italic><0.01). In addition,the content of Cr(<italic>P</italic><0.01) in the powder group and Cr(<italic>P</italic><0.01),Pb(<italic>P</italic><0.05) in the water decoction group were significantly reduced. Conclusion:The powder and water decoction of sedimentary type Limonitum had definite and positive intervention effect on warfarin hemorrhage model rats,which could play a coagulation role by enhancing the vasoconstriction ability,promoting the activation of platelets,and increasing the platelet aggregation rate and blood viscosity. The metal elements such as Na,K,Ca,Fe,Li,Ti,V,Co,Cu,Zn,Ni and Sr may be the material basis for sedimentary type Limonitum to exert hemostatic effect. According to the above indicators,the intervention effect of powder group and decoction group was basically the same.

7.
Article in Chinese | WPRIM | ID: wpr-906489

ABSTRACT

Objective:To explore the possible mechanism of Chloriti Lapis in the treatment of epilepsy by the metabonomics of brain tissue in pentylenetetrazol (PTZ)-kindled epileptic rats treated with Chloriti Lapis. Method:The epileptic animal model in rats was established by PTZ kindling, and the rats were divided into the control group, model group, carbamazepine group and Chloriti Lapis group. The brain tissue samples were detected by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC/Q-TOF-MS), and the experimental results were statistically analyzed by partial least squares-discriminant analysis (PLS-DA) and SPSS 18.0. Result:The metabolic fingerprints and metabolic profiles of the rat brain tissue were established, which showed that the metabolic profiles of each group had changed significantly and could be separated well among the groups. Moreover, the Chloriti Lapis group had a tendency to be closer to the control group than the carbamazepine group. Seven differential metabolites were screened, including phosphatidylserine (PS) (18∶0/18∶0), <italic>L</italic>-glutamic acid, docosahexaenoyl ethanolamide, arachidonic acid, glucosylsphingosine, cholestane-3,7,12,24,25-pentol and lysophosphatidylcholine (LysoPC) (P-18∶0). Except for docosahexaenoyl ethanolamide and LysoPC (P-18∶0), Chloriti Lapis had significant intervening and regulating effects on the other five differential metabolites. There were 12 possible metabolic pathways that affected the metabolic disorder of PTZ-kindled rats, and 3 important metabolic pathways (pathway impact>0.1), namely, <italic>D-</italic>glutamine and <italic>D-</italic>glutamate metabolism, alanine, aspartate and glutamate metabolism, and arachidonic acid metabolism, among which <italic>D-</italic>glutamine and <italic>D-</italic>glutamate metabolism was the most important metabolic pathways. Conclusion:From this point of view, Chloriti Lapis has a clear intervention effect on PTZ-kindled epileptic rats, which may be related to the intervention of the above differential metabolite contents and related metabolic pathways. It can reduce the toxic effect of excitatory neurotransmitters on neurons in brain tissue and inhibit the development of inflammation in brain tissue, so as to maintain the biological function of brain cells and slow down the occurrence of epilepsy.

8.
Article in Chinese | WPRIM | ID: wpr-906433

ABSTRACT

Objective:To investigate the effect of Chloriti Lapis on metal elements in brain tissue and plasma of epileptic rats kindled by pentylenetetrazol (PTZ), and to explore the possible material basis of Chloriti Lapis. Method:PTZ kindling method was used to establish epileptic rat model. Inductively coupled plasma mass spectrometry (ICP-MS) and inductively coupled plasma optical emission spectrometer (ICP-OES) were used to determine the contents of metal elements in brain tissue and plasma of the blank group, model group, carbamazepine group (0.1 g·kg<sup>-1</sup>) and Chloriti Lapis group (2 g·kg<sup>-1</sup>). The data were statistically analyzed by SPSS 18.0 software. Result:Compared with the blank group, the contents of Sr, Sb and Ba in brain tissue of rats in the model group were significantly increased (<italic>P</italic><0.05, <italic>P</italic><0.01), while the contents of Zn, Fe, Cu, K, Li, Co, Sn and Pb were significantly decreased (<italic>P</italic><0.05, <italic>P</italic><0.01). Compared with the model group, the contents of Zn, Fe, K, Li, Co, As and Pb in brain tissue of rats in the Chloriti Lapis group were obviously increased (<italic>P</italic><0.05, <italic>P</italic><0.01), while the contents of Sr and Sb were significantly decreased (<italic>P</italic><0.01). These results showed that Chloriti Lapis had positive effect on the regulation of the content of metal elements in rat brain tissue to normal level, the intervention effect was clear, and the overall effect was better than that of carbamazepine group. The determination of 21 metal elements in plasma showed that compared with the blank group, the levels of K, Sr and Cd in the model group were significantly increased (<italic>P</italic><0.05), and the contents of Li, Al, Ti and Cr were significantly decreased (<italic>P</italic><0.05). Compared with the model group, the contents of Ca, K, Li, Al and V in the Chloriti Lapis group were obviously increased (<italic>P</italic><0.05, <italic>P</italic><0.01), and the contents of Fe, Ti, Sr and Cd were significantly decreased (<italic>P</italic><0.05,<italic>P</italic><0.01). The correlation analysis of metal elements among the groups showed that there were 17 pairs of elements had positively correlation in the brain tissue of rats, 2 pairs of elements had significant negative correlation. In the plasma of rats, 8 pairs of elements had significant positive correlation and 6 pairs of elements had significant negative correlation. Conclusion:The metal element groups represented by Zn, Fe, K, Li, Co, As, Pb, Sr, Sb, Ca, Al, V, Ti and Cd may be the effective material basis for Chloriti Lapis to interfere PTZ-kindled epileptic model rats, which may be related to the influence of these metal element groups on the release of neurotransmitters and the electrical balance of neurons, the regulation of abnormal synchronous discharge induced by Na<sup>+</sup>, K<sup>+</sup>, Ca<sup>2+</sup> channel disorders and intervention of metabolism pathways in brain tissue related to epilepsy. It can make the excitatory and inhibitory activities restrain each other, and finally reach the normal physiological state of neurons and cells. The intervention effect of Chloriti Lapis group was better than that of carbamazepine group.

9.
Article in Chinese | WPRIM | ID: wpr-906151

ABSTRACT

Objective:To evaluate of modified Shentong Zhuyutang combined with celecoxib capsules in the treatment of acute discogenic low back pain due to wind-cold and stasis obstruction sydrome. Method:One hundred and forty eligible patients were randomly divided into control group (70 cases) and observation group (70 cases). Patients in both groups took the celecoxib capsules orally, 200 mg/time. On this basis, patients in the control group were further treated with Tongluo Huoxue capsule orally, 6 capsules/time, 3 times/day, while those in the observation group received the modified Shentong Zhuyutang, 1 bag/day, for three weeks. The short-form McGillpain questionnaire (SF-MPQ), including pain rating index (PRI), present pain intensity (PPI), and visual analog scale (VAS), Oswestry disability index (ODI), wind-cold obstruction and stasis syndrome, and abilities of daily living (ADL) scoring and the Schober's test were conducted before and after treatment. The tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin-1<italic>β</italic> (IL-1<italic>β</italic>), IL-6, thromboxane B<sub>2</sub> (TXB<sub>2</sub>), and 6-keto prostaglandin F<sub>1</sub><italic><sub>α</sub></italic> (6-keto-PGF<sub>1</sub><italic><sub>α</sub></italic>) levels before and after treatment were measured, followed by the safety evaluation. Result:The PRI, VAS, and PPI scores in SF-MPQ of the observation group were reduced in contrast to those in the control group (<italic>P</italic><0.01). The wind-cold and stasis obstruction syndrome and ODI scores in the observation group also declined as compared with those of the control group (<italic>P</italic><0.01), while the ADL and Schober's test scores were elevated (<italic>P</italic><0.01). The TNF-<italic>α</italic>, IL-1<italic>β</italic>, IL-6, and TXB<sub>2</sub> levels in the observation group were lower than those in the control group (<italic>P</italic><0.01), whereas the 6-keto-PGF<sub>1</sub><italic><sub>α</sub></italic> was higher (<italic>P</italic><0.01). The excellent and good rate of the observation group was (51/63) 80.95%, significantly better than (39/63) 61.90% of the control group (<italic>χ</italic><sup>2</sup>=5.601, <italic>P</italic><0.05). No adverse reactions occurred after the intake of Chinese medicinal prescriptions. Conclusion:The modified Shentong Zhuyutang combined with celecoxib capsules is efficient and safe in inhibiting inflammation, easing back pain, enhancing lumbar spine mobility, and improving the ADL of patients with acute DLBP of wind-cold obstruction syndrome.

10.
Article in Chinese | WPRIM | ID: wpr-906115

ABSTRACT

Objective:To discuss the clinical efficacy of modified Bushen Huoxuetang combined with autologous bone grafting and locking compression plate (LCP) in treating nonunion of long bone fractures, and the effect on microcirculation, osteogenic differentiation factor and bone metabolism index. Method:A total of 70 patients were randomly divided into control group and observation group by random number table, with 35 cases in each group. Patients in both groups received LCP. Patients in control group got Dieda Shenggu granule, 10 g/time, 1 time/day. Patients in observation group got Bushen Huoxuetang, 1 dose/day. The course of treatment lasted for 3 months, and 3-month follow-up data were recorded. On a weekly basis, the main symptoms, such as pain, tenderness, longitudinal percussion pain and swelling were checked, and the time of disappearing of main symptoms and signs were compared. On a weekly basis, a X-ray examination was performed for callus formation and fracture line, and the fracture healing time was recorded. Before and after treatment, Fugl-Meyer (FMA) was scored, and levels of fibrinogen (FIB), whole blood viscosity (BV) (high shear, low shear), plasma viscosity (PV), platelet aggregation rate (PAR), <italic>D</italic>-Dimer (<italic>D</italic>-D), bone morphogenetic protein-2 (BMP-2), BMP-7, insulin-like growth factor-1 (IGF-1), vascular endothelial growth factor (VEGF), transforming growth factor-<italic>β</italic><sub>1</sub> (TGF-<italic>β</italic><sub>1</sub>), osteocalcin (BGP), osteoprotegerin (OPG), procollagen type Ⅰ N-terminal propeptideserum amino pro peptide (PINP), serum type 1 collagen cross-linked C-terminal peptide (S-CTX) and serum tartrate resistant acid phosphatase (TRACP) of type I procollagen were detected, and the safety was evaluated. Result:Disappearance time of symptoms and signs and fracture healing time in observation group were all lower than those in control group (<italic>P</italic><0.01). At the third month after treatment, and during the three-month follow-up, scores of callus and FMA (upper and lower limbs) in observation group were all higher than those in control group (<italic>P</italic><0.01). Levels of <italic>D</italic>-D, FIB, PAR, BV and PV (high-cut and low-cut), BMP-2, BMP-7, IGF-1, VEGF, TGF-<italic>β</italic><sub>1</sub>, S-CTX and TRACP were all lower than those in control group (<italic>P</italic><0.01), whereas levels of BGP, OPG and PINP were higher than those in control group (<italic>P</italic><0.01). The curative effect of fracture healing was better than that of control group (<italic>Z</italic>=1.977, <italic>P</italic><0.05). And the limb function recovery was superior to that in control group (<italic>Z</italic>=1.970, <italic>P</italic><0.05). Conclusion:Based on autogenous bone and LCP, modified Bushen Huoxuetang can promote the fracture healing, shorten the course of disease, and promote the recovery of limb function, with a good clinical efficacy. It can improve microcirculation, promote the expression of osteogenic differentiation factor, regulate bone metabolism, and play a role in promoting fracture healing, with a safety in clinical use.

11.
Article in Chinese | WPRIM | ID: wpr-888053

ABSTRACT

To study the effect of mineral Chloriti Lapis on pulmonary metabolites and metabolic pathways in lung tissues of rats with acute exacerbation of chronic obstructive pulmonary disease(AECOPD). The AECOPD rat model of phlegm heat syndrome was replicated by the method of smoking combined with Klebsiella pneumoniae infection. Except for using UPLC-Q-TOF-MS analysis, SPSS 18.0, SIMCA 13.0 and other software were also used for statistical analysis. Through literature search and online database comparison, the differential metabolites were identified, and the possible metabolic pathways were analyzed. After 15 days of administration, PLS-DA analysis was carried out on lung tissue samples of rats in each group. The results showed that the metabolic profiles of lung tissues of rats in each group could be well separated, which indicated that Chloriti Lapis and aminophylline had significant intervention effect on the lung metabolic profile of rats with AECOPD. Moreover, the metabolic profile of Chloriti Lapis group was closer to that of control group, and the intervention effect was better than that of aminophylline group. As a result, 15 potential differential metabolites were identified: phytosphingosine, sphinganine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], lysoPC(18∶0), stearic acid, lysoPC(15∶0), arachidonic acid, docosapentaenoic acid, linoleic acid and palmitic acid. Among them, Chloriti Lapis could significantly improve the levels of 10 differential metabolites of phytosphingosine, tetradecanoylcarnitine, L-palmitoylcarnitine, elaidic carnitine, lysoPC[18∶2(9Z,12Z)], lysoPC(16∶0), lysoPC[18∶1(9Z)], stearic acid, lysoPC(15∶0), and palmitic acid(P<0.05). The intervention effect of Chloriti Lapis group was better than that of aminophylline group. Analysis of metabolic pathways showed that there were 8 possible metabolic pathways that could be affected, and three of the most important metabolic pathways(pathway impact>0.1) were involved: linoleic acid metabolism, arachidonic acid metabolism, and sphingolipid metabolism. Chloriti Lapis had obvious intervention effects on lung tissue-related metabolites and metabolic pathways in rats with AECOPD, and the effect was better than that of aminophyllinne.


Subject(s)
Animals , Lung , Medicine, Chinese Traditional , Metabolomics , Minerals , Pulmonary Disease, Chronic Obstructive , Rats
12.
Article in Chinese | WPRIM | ID: wpr-888023

ABSTRACT

The effects of Chloriti Lapis on metal elements in plasma and lung tissue of acute exacerbation of chronic obstructive pulmonary disease( AECOPD) rats were studied. The rat AECOPD model with phlegm heat syndrome was established by smoking combined with Klebsiella pneumoniae infection. After the rats were treated by Chloriti Lapis,the contents of metal elements in plasma and lung tissue were determined by inductively coupled plasma-optical emission spectroscopy( ICP-OES) and inductively coupled plasma mass spectrometry( ICP-MS). The changes in the contents of metal elements were analyzed by SPSS 18. 0. Further,the correlations of differential metal elements( including Cu/Zn ratio) with differential metabolites in plasma,lung tissue and urine of AECOPD rats treated with Chloriti Lapis were analyzed. The results showed that Chloriti Lapis significantly up-regulated the contents of Fe,Al,Mn,Cu,Zn,Sn( P<0. 05),V,Co( P< 0. 01) and Cu/Zn ratio( P< 0. 05),and significantly down-regulated the contents of Ti( P< 0. 05)and Pb( P<0. 05) in the model rat plasma. It significantly increased the content of Be( P<0. 05) and decreased the contents of Mg,Ti and Al( P<0. 01) in model rat lung tissue. The element profiles of normal group,model group and Chloriti Lapis group can be well separated. Chloriti Lapis group and other groups were clustered into two categories. The taurine in plasma and phytosphingosine in lung tissue had the strongest correlations with differential metal elements. The Fe,Al,Mg,Be,Ti,V,Mn,Cu,Zn,Sn,and Co in Chloriti Lapis may directly or indirectly participate in the intervention of AECOPD rats. This group of metal elements may be the material basis of Chloriti Lapis acting on AECOPD rats,and reduce the Cu/Zn value in vivo. It was further confirmed that Chloriti Lapis could interfere with the metabolic pathways of taurine and hypotaurine in plasma and urine as well as the sphingolipid metabolism pathway in lung tissue of AECOPD rats. In addition,this study confirmed that long-term smoking can cause high-concentration Cd accumulation in the lung and damage the lung tissue.


Subject(s)
Animals , Lung , Medicine, Chinese Traditional , Minerals , Pulmonary Disease, Chronic Obstructive , Rats , Spectrum Analysis , Trace Elements/analysis
13.
Article in Chinese | WPRIM | ID: wpr-879171

ABSTRACT

Metallomics is a frontier interdisciplinary subject at its vigorous development stage. Its goal is to systematically study the content, distribution, chemical species, structural characteristics and functions of metal elements in biological system. It is also a comprehensive discipline to study the existing state and function of free or complex metal elements in life. Metallomics is an ideal tool to study the biological behavior of inorganic elements, which can be used to solve many problems in the research of mineral Chinese medicine(MCM). It provides a strong theoretical basis and technical support for the research of MCM. Its theory and methods provide re-ference and enlightenment for the in-depth study of MCM, and also provide new ideas and open up new ways for the research of MCM. The application of metallomics theory and methods in the research of MCM is of great significance to reveal the material basis and mec-hanism of MCM, promote the process of basic research on MCM, fully exploit and utilize medicinal mineral resources and carry forward the traditional MCM treasure in China. In this paper, we introduced the concept, academic development, research content and research methods of metallomics, and discussed the application prospects of metallomics in the analysis of inorganic element composition characteristics and quality control, material basis and mechanism of MCM, so as to provide reference for further researches on MCM.


Subject(s)
China , Drugs, Chinese Herbal , Medicine, Chinese Traditional , Minerals , Quality Control
14.
Article in Chinese | WPRIM | ID: wpr-878719

ABSTRACT

Objective To investigate the effects of self-made carriers on the cryopreservation of ovarian tissue of sheep. Methods Thirty-two ovaries were randomly assigned to fresh group,programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group.The morphology,proliferation,apoptosis,and estrogen level of the ovarian tissue in each group were observed. Results After cryopreservation,the morphology normal rate of the primordial follicles in programmed freezing group,self-made carrier I vitrification group,and self-made carrier Ⅱ vitrification group were 74.2%,72.8%,and 72.3%,respectively,lower than that(83.7%)in the fresh group(χ


Subject(s)
Animals , Cryopreservation , Female , Freezing , Ovarian Follicle , Ovary , Sheep , Vitrification
15.
Chinese Medical Journal ; (24): 1276-1285, 2021.
Article in English | WPRIM | ID: wpr-878166

ABSTRACT

Excessive consumption of fructose, the sweetest of all naturally occurring carbohydrates, has been linked to worldwide epidemics of metabolic diseases in humans, and it is considered an independent risk factor for cardiovascular diseases. We provide an overview about the features of fructose metabolism, as well as potential mechanisms by which excessive fructose intake is associated with the pathogenesis of metabolic diseases both in humans and rodents. To accomplish this aim, we focus on illuminating the cellular and molecular mechanisms of fructose metabolism as well as its signaling effects on metabolic and cardiovascular homeostasis in health and disease, highlighting the role of carbohydrate-responsive element-binding protein in regulating fructose metabolism.


Subject(s)
Fructose/adverse effects , Homeostasis , Humans , Metabolic Diseases/etiology
16.
Article in English | WPRIM | ID: wpr-880748

ABSTRACT

The present study was conducted to clarify the therapeutic effect of cornuside on experimental autoimmune encephalomyelitis (EAE) and its influence on T helper 17 (Th17) cell and regulatory T (Treg) cell infiltration into the central nervous system. Rats were randomly placed into four treatment groups: control, EAE, EAE+cornuside, and EAE+prednisolone. The neurological function scores of rats were assessed daily. On the second day after EAE rats began to show neurological deficit symptoms, the four groups were treated with normal saline, normal saline, cornuside (150 mg/kg), and prednisolone (5 mg/kg), respectively. The treatment was discontinued after two weeks, and the spinal cord was obtained for hematoxylin and eosin (H&E) and luxol fast blue staining, as well as retinoic acid receptor-related orphan receptor γ (RORγ) and forkhead box protein P3 (Foxp3) immunohistochemical staining. Blood was collected for Th17 and Treg cell flow cytometry testing, and the serum levels of interleukin (IL)-17A, IL-10, transforming growth factor-β (TGF-β), IL-6, IL-23, and IL-2 were measured via enzyme-linked immunosorbent assay (ELISA). Compared with rats in the EAE group, rats in the EAE+cornuside and EAE+prednisolone groups began to recover from neurological deficits earlier, and had a greater degree of improvement of symptoms. Focal inflammation, demyelination, and RORγ-positive cell infiltration were reduced by cornuside or prednisolone treatment, whereas the Foxp3-positive cell numbers were not significantly different. Meanwhile, the number of Th17 cells and the IL-17A, IL-6, and IL-23 levels were lower in the blood after cornuside or prednisolone treatment, whereas the number of Treg cells or the levels of IL-10, TGF-β, and IL-2 were not markedly different. Cornuside can alleviate symptoms of EAE neurological deficits through its anti-inflammatory and immunosuppressive effects, and Th17 cells may be one of its therapeutic targets.

17.
Article in Chinese | WPRIM | ID: wpr-905779

ABSTRACT

Objective:To investigate the effects of combination of transcranial direct current stimulation (tDCS) and mirror therapy (MT) on upper limb motor function after stroke. Methods:From April, 2018 to March, 2019, 78 stroke inpatients were randomly assigned into tDCS group (n = 26), MT group (n = 26) and combined group (n = 26). All the patients received their related therapy, respectively, based on routine medicine and rehabilitation. Their motor-evoked potential cortical latency (CL) and central motor conduction time (CMCT) in affected brain area were measured before and four weeks after treatment, while they were assessed with Fugl-Meyer Assessment-Upper Extremity (FMA-UE) and modified Barthel Index (MBI). Results:All CL, CMCT, and the scores of FMA-UE and MBI improved significantly in all the groups after treatment (|t| > 2.609,P < 0.05), and improved more in the combined group than in both tDCS group and MT group (P < 0.05). Conclusion:Both tDCS and MT could improve excitability of cerebral cortex of the affected brain area and promote the recovery of upper limb motor function for patient after stroke, and it is more effective in combination mode.

18.
Chinese Journal of Biotechnology ; (12): 1241-1248, 2020.
Article in Chinese | WPRIM | ID: wpr-826853

ABSTRACT

The opening and sharing of large-scale life science equipment in universities is expanding. We must find ways to improve the role of large-scale equipment in cultivating students' practical and innovative abilities, to tap the potential of equipment, then to support scientific research and speed up scientific research output. We established a set of large-scale equipment training and practice teaching system including 15 topics and covering a wide range of technologies. In practice, we constantly innovated personalized courses. According to the differences of students' major and scientific research needs, we classified teaching content and set up parallel classes. Each class had the individualized teaching content and students could select from a menu of courses. In addition, we built up a cloud classroom teaching platform, online and offline teaching method supplemented each other. The teaching system have produced certain effect.


Subject(s)
Biological Science Disciplines , Education , Curriculum , Reference Standards , Humans , Students , Universities
19.
Article in Chinese | WPRIM | ID: wpr-847932

ABSTRACT

BACKGROUND: With the development of Chinese space industry, pilots are exposed to high-G mechanical environment, which will seriously affect their bones. However, as one of the bones that are most prone to fracture, the biomechanical behavior of the tibia under extreme mechanical environment is rarely reported. OBJECTIVE: To explore the effects of different high-G mechanical environments on the rat growth and development and the mechanical properties of the tibia. METHODS: Male Wistar rats were provided by Laboratory Animal Center of Academy of Military Sciences. The cantilever was set to run at different speeds and accelerations with a high-G centrifugal loading device, and the high-G rat model was made. The rats were weighed weekly. The left tibia of rats was used for undergoing a three-point bending experiment, and the deflection and elastic modulus of the tibia were calculated. The right tibia was subjected to a creep test, and constant stress was applied on the surface of tibial cortical bone and kept 3 600 seconds, so as to observe the changes of creep strain. The study was approved by the Laboratory Animal Ethical Committee of Tianjin University of Technology. RESULTS AND CONCLUSION: The high-G environment affected the normal growth and development of rats, inhibited weight gain and reduced the mechanical properties of the tibia, and reduced the limit deflection of the tibia by 8.1%, 12.2%, 37.8%, and 51.4%, respectively. Limit loads were decreased by 16%, 9%, 25.2%, and 29% respectively. To conclude, extremely high-G environment exerts serious negative effects on rats.

20.
Article in Chinese | WPRIM | ID: wpr-872992

ABSTRACT

Objective::To analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and polycystic ovary syndrome (PCOS) rats, and to explore the effect of PCOS on the metabolism of Shaoyao Gancaotang. Method::Female SD rats were randomly divided into normal group and PCOS group. PCOS rat model was prepared by administration of letrozole solution for 21 days, and the estrous cycle of rats was observed. UHPLC-QTRAP-MS/MS technique and LightSight 2.3 software were used to analyze and identify the metabolites of major components of Shaoyao Gancaotang in urine and feces of normal and PCOS rats. The mobile phase was water-methanol for gradient elution, the flow rate was 0.3 mL·min-1, electrospray ion source was employed under negative ion mode. Result::Phase Ⅰ and phase Ⅱ reactions mainly occurred in drug metabolism. A total of 27 metabolites were detected in urine of normal rats, and 34 metabolites were detected in urine of PCOS rats. A total of 29 metabolites were detected in feces of normal rats, and 27 metabolites were detected in feces of PCOS rats. Conclusion::The metabolites in the urine of PCOS rats are more diverse than those of normal rats, and the disease status of PCOS may affect the in vivo metabolic pathway of active ingredients in Shaoyao Gancaotang.

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