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1.
Acta Pharmaceutica Sinica ; (12): 741-749, 2022.
Article in Chinese | WPRIM | ID: wpr-922894

ABSTRACT

The crude Et2O extract of soft coral Sarcophyton glaucum, collected off the Xisha, the South China Sea, were investigated. A new cembrane-type diterpenoid, namely 15-dehydroxy-sarcophytrol D (1), together with twenty-five known compounds, namely ximaoglaucumin C (2), (11S,12S,1E,3E,7E)-11,12-epoxycembra-1,3,7-triene (3), sarcophytol W (4), cembrene (5), sarcophytol B (6), sarcophytol K (7), sarcophytol J (8), pentaene-cembrene (9), sarcophytol E (10), (+)-marasol (11), (2S)-sarcophytoninsarcophytoxide (12), (-)-17-hydroxydeepoxysarcophytoxide (13), (+)-sarcophytoxide (14), 13-acetoxysarcophytoxide (15), bophynin B (16), 16-oxosarcophytoxide E (17), sarcophinone (18), 7α-8β-dihydroxydeepoxysarcophine (19), (+)-sarcophine (20), 14-dehydroxy-sarcophytol L (21), sarcophytol L (22), 13α-hydroxy-sarcophytol L (23), trocheliophol C (24), trocheliophol E (25) and trocheliophol L (26), were isolated and purified by comprehensive chromatography methods of silica gel column, Sephadex LH-20 gel column, TLC, and semi-preparative high-performance liquid chromatography (HPLC). In anti-inflammatory bioassay, compound 4 exhibited inhibitory effects on lipopolysaccharide (LPS)-induced inflammatory responses in BV-2 microglial cells.

2.
Article in Chinese | WPRIM | ID: wpr-816217

ABSTRACT

OBJECTIVE: To study the expression of transient receptor potential canonical-6(TRPC6)channels in endometrial cancer tissues and their role in regulating proliferation of endometrial cancer cells.METHODS: Thirty patients with endometrial cancer who were treated from January 2011 to June 2015 in Henan Provincial People's Hospital and Xuchang People's Hospital were chosen as the study group;the controls group consisted of 24 atypical hyperplasia patients and 28 uterus leiomyoma patients.Molecular biological techniques were used to examine the expression of TRPC6 channels in 30 endometrial cancer specimens,24 atypical hyperplasia specimens and 28 normal endometrial specimens.SKF96365(an inhibitor of TRPC6 channel)and siRNA interference(RNAi)targeting TRPC6 channel were used to block TRPC6 so as to explore the role of TRPC6 channels in regulating the proliferation of endometrial cancer cells by[3 H]thymidine incorporation and cell number.RESULTS: The expression levels of TRPC6 in endometrial cancer were notably elevated than those in the atypical hyperplasia endometrial and normal endometrial tissues. The expression levels of TRPC6 in endometrial cancer vs. the control:mRNA:(0.98±0.56)vs.(0.30±0.24 and0.23±0.13)(P<0.01):protein:(1.22±0.39)vs.(0.75±0.27 and 0.73±0.26)(P<0.01);The expression level of TRPC6in endometrial cancer tissues was not related to the surgical pathological staging, but was related to pathological staging; SKF96365 caused a dosedependent decline in cell amount of HEC-1 A cell. The expression quantity of TRPC6 in whole lysates of the celltransfected with target-TRPC6 small interference RNA(siRNA)was(38.51±6.21)% of that found in the cells transfected with non-silencing RNA;[3 H]thymidine incorporation in HEC-1 A transfected with target-TRPC6 siRNA was also reduced,siRNA inhibited HEC-l A cells proliferation,compared with the cells transfected with non-silencing RNA.CONCLUSION:s TRPC6 channels mignt be closely related to the proliferation of endometrial cancer cells and down regulation of its expression may suppress its development.

3.
Article in Chinese | WPRIM | ID: wpr-774024

ABSTRACT

Objective To assess the value of CT angiography using low-voltage and low-concentration contrast media (CM) combined with knowledge-based iterative model reconstruction (IMR) in patients with coronary artery bypass grafts (CABG).Methods Totally 71 patients after CABG undergoing CT angiography in our center from June to November 2016 were prospectively enrolled and randomly assigned into groups A and B. The scan protocol for group A was 80 kVp with 300 mgI/ml contrast at an injection rate of 4 ml/s;images were reconstructed by IMR algorithm. The scan protocol for group B was 100 kVp with 370 mgI/ml contrast at an injection rate of 5 ml/s;images were reconstructed by hybrid iterative reconstruction technique. Aorta,left ventricular,and grafts were chosen as regions of interest. The image quality,radiation dose,and contrast load were compared between two groups.Results The signal to noise ratio (SNR) and contrast to noise ratio (CNR) of the ascending aorta,descending aorta,left ventricular,and venous bridge in group A [SNR:19±5,20±5.7,19.1±4.9,and 37±34;CNR:17±4.7,18±5,16±5.4,and 34±32] were significantly higher than those in group B [SNR:16±6 (P=0.012),15.6±5.5 (P=0.002),15±6 (P=0.002),24±8.3 (P=0.035);CNR:14±5.5 (P=0.010),13.8±5(P=0.002),13±5.7 (P=0.014),21±7.8 (P=0.031)],except for left internal mammary artery graft (LIMA),which was not inferior to that in group B. An effective radiation dose reduction of 49% was achieved in group A [(2.3±0.4) mSv,compared with group B (4.5±0.5) mSv (P=0.000)]. The iodine load of group A was (20±1.4) g compared with (29±1.6) g in group B,resulting in a reduction of 31% (P=0.000).Conclusions The low tube voltage (80 kVp) and low contrast protocol combined with IMR in patients with CABG can reduce radiation dose and improve image quality of aorta,left ventricular and venous graft. The image quality of LIMA graft in low dose group is not inferior to that in regular dose group.


Subject(s)
Computed Tomography Angiography , Contrast Media , Coronary Angiography , Coronary Artery Bypass , Humans , Radiation Dosage , Radiographic Image Interpretation, Computer-Assisted
4.
International Eye Science ; (12): 702-705, 2018.
Article in Chinese | WPRIM | ID: wpr-695284

ABSTRACT

·AIM: To explore clinical effect of pranoprofen combined with deproteinized calf blood extract eye drops for moderate to severe dry eye. ·METHODS: A total of 84 patients (132 eyes) who received treatment at the Zhengzhou Second Hospital were selected from January 2016 to January 2017. According to random number table method they were divided into control group 42 cases (68 eyes) and observation group 42 cases (64 eyes), the control group using polyvinyl alcohol eye drops with pranoprofen, observation group with pranoprofen with deproteinized extract of calf blood eye drops. Subjective and objective scores before and after treatment were recorded. · RESULTS: There was no statistically significant difference on the four objective indicators of pretreatment FL,BUT,SⅠt,and vision between the two groups (P>0.05). Dry eye symptom scores of the two groups decreased after treatment,both with significantly different (P<0.05);after treatment,the symptom scores of the observation group were significantly lower than those in the control group, and the difference was statistically significant (P< 0. 05), indicating that the subjective symptoms of the observation group were better than that of the control group after treatment. After treatment of the control group and the observation group,the FL index was significantly lower than before treatment, BUT, S Ⅰ t and vision were significantly higher than before treatment, and the differences within either group were statistically significant(P<0.05);those indexes of observation group were different compared with control group (P < 0. 05), indicating that the objective symptoms of the observation group were better than that of the control group after treatment. None of the patients showed serious adverse reactions. ·CONCLUSION: The clinical effect of praprofen on the treatment of moderate to severe dry eye with the deproteinized calf blood extract is better.

5.
Article in English | WPRIM | ID: wpr-687949

ABSTRACT

To identify the risk factors that are associated with the midterm coronary artery bypass grafting (CABG) functionality by assessing patency of left internal mammary artery (LIMA) graft and saphenous vein (SV) graft with 64-slice multi-detector computed tomography (64-MDCT).Methods Patients who underwent CABG operation and postoperative 64-MDCT follow-up examinations from August 2012 to December 2015 were included. The graft patent status was classified into patent and poor patent according to MDCT findings predominantly on 3D reconstructed images by two radiologists. The clinical data and imaging findings of the patients were collected and compared between the patent group and poor patent group. Univariate analysis and the multivariate logistic regression analysis were performed to identify the risk factors that affect graft patency.Results Among 341 patients in the study, there were 330 LIMA grafts [326 anastomosed to the left anterior descending artery (LAD), 4 to right coronary artery (RCA)] and 564 SV grafts (SVG) [100 anastomosed to the diagonal branch (D), 226 to the obtuse marginal branch (OM), and 238 to the RCA territory]. The approximal vessel stenosis exceeding 90% occurred in 268 of 292 patent LIMA grafts, and in 1 of 34 poor patent grafts (χ =167, P<0.001). The patency rate was higher when SVG was anastomosed to OM (85.4%) or RCA territory (81.9%) than to D (69.0%) (χ =15.471, P=0.004). The proximal target vessel stenosis < 90% (OR= 0.015, 95% CI: 0.01-0.14, P=0.000) was independently associated with the closure risk of LIMA grafts, the dyslipidemia (OR= 1.52, 95% CI: 1.0-2.5, P=0.048), history of diabetes (OR = 1.28, 95% CI : 0.90-2.26, P=0.045) and typical angina symptoms (OR=1.81, 95% CI :1.33-4.15, P=0.003) were independently associated with the closure risk of SVG. Conclusions The proximal LAD stenosis less than 90% was adversely associated with graft patency in LIMA recipients; dyslipidemia, diabetes and angina symptoms were associated with the midterm failure in SVG recipients. The choice of the target anastomosis sites may affect the patency of SVG.

6.
Mycobiology ; : 311-316, 2018.
Article in English | WPRIM | ID: wpr-729745

ABSTRACT

Amylosporus sulcatus sp. nov. is described from Nonggang Nature Reserve, southern China, on the basis of morphological and molecular data. The morphological description and illustrations for the new species are provided. The species is characterized by pileate and stipitate basidiocarps. The pileus surface is obviously concentrically and radiately sulcate and tomentum, and the pore surface is snow white. Phylogenetic analyses based on sequences of the internal transcribed spacer and nuclear large subunit ribosomal DNA confirmed it to be a new species.


Subject(s)
China , Classification , DNA, Ribosomal , Fruiting Bodies, Fungal , Snow
7.
Military Medical Sciences ; (12): 968-972,977, 2017.
Article in Chinese | WPRIM | ID: wpr-694290

ABSTRACT

Objective To investigate the therapeutic effect of interleukin-2(IL-2)on experimental autoimmune encephalomyelitis(EAE)mice.Methods After establishment of the EAE(experimental autoimmune encephalomyelitis) mouse models with MOG35-55 polypeptides,the mice were grouped according to the neurological function score and divided into control group,EAE group and low dose IL-2 treatment group.A double blind method was used to evaluate the neuro-logical impairment in mice.On the 29th day,pathological experiments were carried out in the mice's brain and spinal cord, hematoxylin-eosin staining was used to evaluate the scoring of inflammatory cell infiltration and luxol fast blue staining was used to evaluate the scoring of demyelinating.The proportion of regulatory T cells(Treg)and NK cells(natural killer cell, NK)was detected by flow cytometry,and the immunohistochemical method was used to detect the expressions of glial fibril -lary acidic protein(GFAP)and myelin basic protein(MBP)in the spinal cord.Results Compared with the EAE group, the neurological function score, the inflammatory cell infiltration score and the demyelinating score of the low dose IL-2 treatment group were reduced.The proportion of Treg cells in the low dose IL-2 treatment group was significantly higher than that in the EAE group,and the proportion of NK cells in the low dose IL-2 treatment group was slightly higher than that in the EAE group The expression of GFAP and MBP was detected by immunohistochemistry.The expression level of GFAP in low dose IL-2 treatment group was significantly lower than that in the EAE group,while the expression level of MBP was higher than that in the EAE group.Conclusion Low dose IL-2 has significant therapeutic effect on EAE mice.

8.
Article in Chinese | WPRIM | ID: wpr-693345

ABSTRACT

Objective To investigate the relationship between methylation of CpG island in glutathione S-transferase P1(GSTP1) promoter region and injury induced by isoniazid in HL-7702 cells.Methods HL-7702 cells were divided into the control group and three isoniazid groups(200,400,800 mg/L).Colorimetric method was used to detect the activity level of lactate dehydrogenase in the medium of HL-7702 cells;the mRNA expression of GSTP1,DNA methyltransferases 1(DNMT1),DNMT3a and DNMT3b were de?tected by real-time fluorescence quantitative PCR;the protein expression levels of DNMT1,DNMT3a and DNMT3b were detected by enzyme-linked immunosorbent assay;the methylation of the CpG island in the GSTP1 promoter region was determined by the bisulfite sequencing PCR.Results The activity level of lactate dehydrogenase in supernatants of the HL-7702 cells in isoniazid group(400, 800 mg/L)was higher than that in the control group(P<0.01).Compared with the control group,the mRNA expression of DNMT 1、3a、3b and GSTP1 were elevated in 400 and 800 mg/L isoniazid groups(P<0.05,P<0.01).The proteins expression of DNMT1 and 3a in the 400 and 800 mg/L isoniazid groups were higher than that in the control group(P<0.05,P<0.01),and the protein expression of DNMT 3b in the 800 mg/L isoniazid groups were higher than that in the control group(P<0.01).The methylation level of CpG is?land in GSTP1 promoter region of three isoniazid groups were decreased.Conclusion The CpG island in the promoter of GSTP1 has hypomethylation in hepatocyte cells damaged by isoniazid.

9.
Article in English | WPRIM | ID: wpr-820221

ABSTRACT

OBJECTIVE@#To discuss the expression and significance of angiostatin, vascular endothelial growth factor and matrix metalloproteinase-9 in the brain tissue of diabetic rats with ischemia reperfusion.@*METHODS@#A total of 60 male Wistar rats were randomly divided into the normal group, sham group, diabetic cerebral infarction group and single cerebral infarction group according to the random number table, with 15 rats in each group. The high sucrose diet and intraperitoneal injection of streptozotocin were performed for the modeling of diabetic rats, while the thread-occlusion method was employed to build the model of cerebral ischemia reperfusion. The immunohistochemical staining was performed to detect the expression of angiostatin, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in the brain tissue.@*RESULTS@#The expression of angiostatin after the reperfusion in the brain tissue of rats in the single cerebral infarction group and diabetic cerebral infarction group was increased 6 h after the reperfusion, reached to the peak on 1 d and then decreased gradually. The expression of angiostatin in the diabetic cerebral infarction group 6 h, 1 d, 3 d and 7 d after the reperfusion was significantly higher than that in the single cerebral infarction group (P < 0.05). VEGF began to be increased 1 h after the reperfusion in the single cerebral infarction group and diabetic cerebral infarction group, reached to the peak at 6 h and then decreased gradually. The expression of VEGF in the diabetic cerebral infarction group at each time point after the reperfusion was significantly lower than that in the single cerebral infarction group (P < 0.05). MMP-9 began to be increased 1 h after the reperfusion in the single cerebral infarction group and diabetic cerebral infarction group, reached to the peak on 1 d and then decreased gradually. The expression of MMP-9 in the diabetic cerebral infarction group at each time point after the reperfusion was significantly higher than that in the single cerebral infarction group (P < 0.05).@*CONCLUSIONS@#The high glucose environment in which the diabetic cerebral infarction is occurred is to induce the formation of MMP-9 at first and then activate and increase the expression of angiostatin. Afterwards, the expression of VEGF is inhibited, resulting in the poor angiogenesis after cerebral infarction, which thus makes the injury of brain tissue after cerebral infarction even worse than the non-diabetes mellitus.

10.
Acta Pharmaceutica Sinica ; (12): 1045-1051, 2015.
Article in Chinese | WPRIM | ID: wpr-257028

ABSTRACT

The objectives of this study are to prepare resveratrol loaded mixed micelles composed of poloxamer 403 and poloxamer 407, and optimize the formulation in order to achieve higher drug solubility and sustained drug release. Firstly, a thin-film hydration method was utilized to prepare the micelles. By using drug-loading, encapsulation yield and particle size of the micelles as criteria, influence of three variables, namely poloxamer 407 mass fraction, amount of water and feeding of resveratrol, on the quality of the micelles was optimized with a central composite design method. Steady fluorescence measurement was carried out to evaluate the critical micelle concentration of the carriers. Micelle stability upon dilution with simulated gastric fluid and simulated intestinal fluid was investigated. The in vitro release of resveratrol from the mixed micelles was monitored by dialysis method. It was observed that the particle size of the optimized micelle formulation was 24 nm, with drug-loading 11.78%, and encapsulation yield 82.51%. The mixed micelles increased the solubility of resveratrol for about 197 times. Moreover, the mixed micelles had a low critical micelle concentration of 0.05 mg · mL(-1) in water and no apparent changes in particle size and drug content were observed upon micelles dilution, indicating improved kinetic stability. Resveratrol was released from the micelles in a controlled manner for over 20 h, and the release process can be well described by Higuchi equation. Therefore, resveratrol-loaded poloxamer 403/407 mixed micelles could improve the solubility of resveratrol significantly and sustained drug release behavior can be achieved.


Subject(s)
Drug Carriers , Chemistry , Fluorescence , Kinetics , Micelles , Particle Size , Poloxamer , Chemistry , Solubility , Stilbenes , Chemistry , Water
11.
Article in Chinese | WPRIM | ID: wpr-286008

ABSTRACT

<p><b>OBJECTIVE</b>To explore the impact of gender on the marginal division(MrD)of the neostriatum in healthy adults.</p><p><b>METHODS</b>Conventional magnetic resonance imaging(MRI),3D structure images,and resting-state function MRI(rs-fMRI)were performed in 64 health adults,who were divided into male group(n=28)and female group(n=26).MrD was defined using manual drawing on structure images,and was applied to the computation of functional connectivity maps.Single group data was performed with simple t test,and two groups data were performed with analysis of covariance with age as the covariance.</p><p><b>RESULTS</b>The brain regions of functional connectivity related with MrD were located in bilateral middle cingulate gyrus,rolandic operculum,insula,putamen,thalamus and amygdala in male group,and in bilateral heschl gyrus,putamen,thalamus and amygdala in female group.The brain regions with increased functional connectivity related with MrD were demonstrated in right superior temporal gyrus,middle temporal gyrus and gyrus rectus,and decreased in left superior parietal cortex in male group compared with that in female group.</p><p><b>CONCLUSION</b>The functional connectivity related with MrD shows certain gender-related consistency and difference in the brain of health adults.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Brain , Physiology , Brain Mapping , Female , Humans , Limbic System , Physiology , Magnetic Resonance Imaging , Male , Middle Aged , Neostriatum , Physiology , Sex Factors , Young Adult
12.
Article in Chinese | WPRIM | ID: wpr-285969

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the changes in subcortical white matter and corpus callosum volumes in patients with type 2 diabetes mellitus (T2DM).</p><p><b>METHODS</b>High resolution three-dimensional T1-weighted fast spoiled gradient recalled echo magnetic resonance images were obtained from 16 T2DM patients and 16 normal controls, and 11 T2DM patients also received the same magnetic resonance imaging scans after insulin therapy for 1 year. Volumetric analysis was performed and analysis of covariance and paired t test were applied.</p><p><b>RESULTS</b>A lower volume was demonstrated in T2DM patients than that in the normal controls in bilateral caudal middle frontal lobe, bilateral pars opercularis, right inferior parietal lobe, right inferior temporal lobe, right middle temporal lobe, and left transverse temporal lobe (all P<0.05). After insulin therapy for 1 year, the increased subcortical white matter of brain regions were demonstrated in left medial orbitofrontal lobe, right caudal middle frontal lobe, right inferior parietal lobe, right lingual lobe, right inferior temporal lobe, right middle temporal lobe, left transverse temporal lobe, left entorhinal lobe and left insular lobe at the follow-up level in T2DM patients compared with baseline level (P<0.05). The decreased volumes of subcortical white matter were observed in the bilateral paracentral lobe, right superior forntal lobe and right precuneus lobe in T2DM patients on insulin therapy for 1 year (P<0.05). A decreased trend of subcortical white matter was involved in corpus callosum (P>0.05) and a significant decreased volume was demonstrated in the middle posterior part of corpus callosum after insulin therapy for 1 year(P<0.05).</p><p><b>CONCLUSIONS</b>T2DM patients have decreased subcortical white matter of multiple brain regions, and the atrophy of subcortical white matter can be improved by the insulin therapy. The volume analysis of subcortical white matter is a simple and effective tool for the evaluation of white matter damage in T2DM patients.</p>


Subject(s)
Aged , Case-Control Studies , Corpus Callosum , Pathology , Diabetes Mellitus, Type 2 , Pathology , Female , Humans , Image Processing, Computer-Assisted , Magnetic Resonance Imaging , Male , Middle Aged , Plethysmography
13.
Chinese Journal of Cardiology ; (12): 397-401, 2012.
Article in Chinese | WPRIM | ID: wpr-275037

ABSTRACT

<p><b>OBJECTIVE</b>This study investigated the impact of metabolic syndrome on the development of cardio-cerebral vascular (CVD) events in a pre-hypertensive population.</p><p><b>METHODS</b>The data used in this prospective study was derived from the Kailuan study cohort (n = 101 510). Prehypertension was diagnosed in 29 968 (mean age: 50 ± 9 years and 23 744 males) individuals by the JNC VII criteria and these subjects were further classified into metabolic syndrome positive (MS+, n = 3447) and MS negative (MS-, n = 26 521) groups according to the modified 2004 Chinese Diabetes Society criteria. Subjects were followed up for 38 - 53 (mean 47 ± 5) months and first-ever CVD events were recorded. Baseline anthropometric and laboratory features were obtained by physical examination from June 2006 to October 2007 and the last follow-up day was December 31, 2010. Multivariable Cox proportional hazards regression models were used to analyze the risk factors of first-ever CVD events.</p><p><b>RESULTS</b>There were 354 CVD events during follow up. The incidences of CVD events (1.80% vs. 1.28%) and cerebral infarction (1.10% vs. 0.57%) were significantly higher in the MS+ group than in the MS- group (all P < 0.05). After adjustment for other established CVD risk factors, the hazards ratio was 1.45 (95%CI: 1.10 - 1.92) for total CVD events and 1.84 (95%CI: 1.27 - 2.67) for cerebral infarction events in MS+ group.</p><p><b>CONCLUSIONS</b>In this cohort, metabolic syndrome is linked with increased risk for CVD events.</p>


Subject(s)
Adult , Cardiovascular Diseases , Cerebrovascular Disorders , Cohort Studies , Female , Humans , Male , Metabolic Syndrome , Middle Aged , Prehypertension , Prospective Studies , Risk Factors
14.
Chinese Journal of Oncology ; (12): 143-146, 2012.
Article in Chinese | WPRIM | ID: wpr-335326

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the effect of anthracycline pirarubicin-based regimen in association with different ways of fluorouracil (5-Fu) as neoadjuvant and adjuvant chemotherapy for primary breast cancer.</p><p><b>METHODS</b>Two hundred and eighty-nine primary breast cancer patients who were to be operated, two to eight cycles of pirarubicin in association with cyclophosphamide and 5-Fu (CTF or CTFci regimen) were given before operation. The pathological response rate, effect and its relation with the infusion routes of 5-Fu were analyzed.</p><p><b>RESULTS</b>The overall pathological complete remission (pCR) rate was 28.4%. The median follow-up period was 39 months. The 5-year DFS was 87.6% (95% CI:82.1% to 92.7%), 5-year DDFS was 89.9% (95% CI:84.0% to 95.8%), and overall survival was 99.6%. CTFci (5-Fu, continuous infusion) regimen was superior to CTF regimen in pCR rates (32.3% vs. 20.2%, P = 0.037), and 5-year DDFS were 92.9% and 80.1%, respectively (P = 0.015). The pCR group was superior to non-pCR group in 5-year DDFS (92.4% vs. 85.6%, P = 0. 033). The pCR rate of patients with ER/PR-positive tumor was significantly lower than those of ER/PR-negative (P = 0.004). The 5-year DDFS rates of HER-2 (+) and HER-2(-) groups were 75.0% and 91.9%, respectively (P = 0.043). In the ER/PR-positve group, the 5-year DDFS of CTFci regimen was superior to those of CTF regimen, 91.4% vs. 81.4% (P = 0.047).</p><p><b>CONCLUSIONS</b>CTF/CTFci regimen as neoadjuvant and adjuvant chemotherapy is effective for primary breast cancer. CTFci regimen is superior to CTF regimen in pathological complete response rate and 5-year DDFS. CTFci regimen may do better to ER/PR (+) patients' benefits compared with CTF regimen.</p>


Subject(s)
Adult , Aged , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Breast Neoplasms , Drug Therapy , Pathology , General Surgery , Carcinoma, Ductal, Breast , Drug Therapy , Pathology , General Surgery , Chemotherapy, Adjuvant , Cyclophosphamide , Disease-Free Survival , Doxorubicin , Female , Fluorouracil , Follow-Up Studies , Humans , Lymphatic Metastasis , Middle Aged , Neoadjuvant Therapy , Neoplasm Metastasis , Neoplasm Recurrence, Local , Proportional Hazards Models , Receptor, ErbB-2 , Metabolism , Receptors, Estrogen , Metabolism , Receptors, Progesterone , Metabolism , Remission Induction , Retrospective Studies , Survival Rate
15.
Article in English | WPRIM | ID: wpr-284362

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate application value of apparent diffusion coefficient (ADC) in differentiating brain radiation-injuries and glioma recurrence.</p><p><b>METHODS</b>Totally 23 patients [18 men and 5 women aged 32 to 67 years (mean: 47 years)] with previously resected and irradiated glioma were examined by using a 3.0T MR Scanner, including conventional and diffusion weighted image (DWI) sequences. All the cases were proved by pathology or clinical follow-up. Postprocessing of ADC maps was performed by using Functool software (AW 4.3, GE Healthcare), and the regions of interest (ROIs) were manually drawn on ADC maps in the area corresponding to the contrast-enhancing area on post-contrast axial T1-weighted images, avoiding the hemorrhage or necrosis. The ADC values were calculated automatically. ADC values were measured 10 times in order to reduce errors, and mean ADC value, maximum ADC value, and minimum ADC value were acquired.</p><p><b>RESULTS</b>Both recurrent glioma and irradiated necrosis tended to have hemorrhage, necrosis, and edema. The mean ADC value and maximum ADC value were lower in the recurrent tumor group than in the radiation-injuries group, but no statistical difference was achieved. However, the minimum ADC value was significantly lower in the recurrent tumor group than in the radiation-injuries group (P=0.016).</p><p><b>CONCLUSION</b>The minimum ADC value can be used as an useful tool in differentiating tumor recurrence from radiation-injuries.</p>


Subject(s)
Adult , Aged , Brain Injuries , Diagnosis , Brain Neoplasms , Diagnosis , Pathology , Diagnosis, Differential , Diffusion Magnetic Resonance Imaging , Female , Glioma , Diagnosis , Pathology , Humans , Male , Middle Aged , Neoplasm Recurrence, Local , Diagnosis , Radiation Injuries , Diagnosis
16.
Chinese Journal of Cardiology ; (12): 415-419, 2010.
Article in Chinese | WPRIM | ID: wpr-341201

ABSTRACT

<p><b>OBJECTIVE</b>To observe the prevalence, blood pressure change in prehypertensive population and associated cardiovascular risk factors.</p><p><b>METHODS</b>Data from a prehypertensive cohort defined with the JNC-7 prehypertension diagnostic criteria were obtained in the employees of kailuan group during the health examination between 2006 to 2007 and the same population was revisited between 2008 to 2009 to observe the change of blood pressure and the associated determinants for blood pressure change.</p><p><b>RESULTS</b>(1) There were 25 474 prehypertensive during the 1(st) visit and 8361 subjects developed hypertension during the 2(nd) visit (35.3% in men and 23.3% in women, 27.2% with baseline blood pressure 120 - 129/80 - 84 mm Hg (1 mm Hg = 0.133 kPa) and 43.8% with baseline blood pressure 130 - 139/85 - 89 mm Hg, 34.3% with risk factors and 19.9% without risk factors). (2) Multiple logistic regression analysis showed that the baseline SBP, waist circumference, age, BMI, gender (male), DBP, TC, FBG, TG, LDL-C were the risk factors of blood pressure progression with a RR (95%CI) of 1.052 (1.048 - 1.056), 1.009 (1.006 - 1.013), 1.023 (1.021 - 1.026), 1.063 (1.052 - 1.074), 1.554 (1.442 - 1.675), 1.036 (1.029 - 1.043), 1.064 (1.037 - 1.093), 1.043 (1.024 - 1.062), 1.041 (1.021 - 1.062) and 1.035 (1.000 - 1.072), respectively.</p><p><b>CONCLUSION</b>A third (32.8%) prehypertensive population progressed into hypertension after two years, baseline SBP, waist circumference, age, BMI, gender (male), DBP, TC, FBG, TG, LDL-C were the risk factors of predicting blood pressure progression.</p>


Subject(s)
Adult , Blood Glucose , Metabolism , Blood Pressure , Cardiovascular Diseases , Epidemiology , Cholesterol , Blood , Female , Follow-Up Studies , Humans , Hypertension , Epidemiology , Male , Middle Aged , Prehypertension , Epidemiology , Prevalence , Risk Factors , Waist Circumference
17.
Acta Physiologica Sinica ; (6): 219-224, 2010.
Article in Chinese | WPRIM | ID: wpr-337756

ABSTRACT

L-glutamate (Glu) is an excitatory neurotransmitter in the mammalian central nervous system. Relatively much attention has been paid to functional expression of Glu signaling molecules in peripheral tissues very recently. The present study tested the hypothesis that the activation of group I metabotropic glutamate receptor (mGluRI) in neutrophils stimulated neutrophils adherence to endothelial cells by increasing the surface expression of certain adhesion molecules. Peripheral blood was obtained by venipuncture from healthy donors, and the neutrophils were isolated by Ficoll-Hypaque gradient centrifugation. Neutrophils floating into DMEM/F12 culture medium containing 10% fetal bovine serum were then used immediately. Immunocytochemistry and real-time quantitative RT-PCR were used to detect the expression of mGluRI (mGluR1 and mGluR5) in neutrophils. The adherence of neutrophils to cultured human normal umbilical vein endothelial cells (HUVE-12) was measured by the colorimetric method. Cell surface expression of adhesion molecule CD11a in the neutrophils was determined by flow cytometry. Immunocytochemistry and real-time quantitative RT-PCR showed that mGluR1 and mGluR5 were constitutively expressed in neutrophils. Application of mGluRI agonist S-3,5-dihydroxyphenylglycine (S-DHPG) (1x10(-8)-1x10(-6) mol/L) showed a dose-dependent stimulatory effect on the adherence of neutrophils to HUVE-12 (P<0.05 or P<0.01), with a maximum effect at 1x10(-6) mol/L (P<0.01). Incubations as short as 30 min were sufficient to induce increased adherence after the beginning of S-DHPG treatment. Following time extension (0.5-5 h), S-DHPG (1x10(-6) mol/L) increased the rate of neutrophils adhesion to HUVE-12 with a maximum effect at 0.5 h (P<0.01). However, a time-dependent effect of S-DHPG on the rate of neutrophils adhesion to HUVE-12 was not observed during the experimental period. 1x10(-6) mol/L of S-DHPG also induced an increased surface expression of adhesion molecule CD11a (P<0.01) when neutrophils were preincubated with 1x10(-6) mol/L of S-DHPG for 1 h. Furthermore, the specific mGluRI antagonist (RS)-alpha-methyl-4-carboxyphenylglycine ((+/-)-MCPG, 0.5 mmol/L) significantly abolished the stimulatory effect of S-DHPG (1x10(-6) mol/L) on the adherence of neutrophils to HUVE-12 (P<0.01). These results suggest that the activation of mGluRI in neutrophils results in increased adhesion molecule CD11a expression and thereby promotes the adherence of neutrophils to endothelial cells.


Subject(s)
Benzoates , Pharmacology , CD11a Antigen , Metabolism , Cell Adhesion , Endothelial Cells , Cell Biology , Glycine , Pharmacology , Human Umbilical Vein Endothelial Cells , Humans , Neutrophils , Cell Biology , Receptor, Metabotropic Glutamate 5 , Metabolism , Receptors, Metabotropic Glutamate , Metabolism , Resorcinols , Pharmacology
18.
Chinese Journal of Surgery ; (12): 1851-1854, 2010.
Article in Chinese | WPRIM | ID: wpr-346397

ABSTRACT

<p><b>OBJECTIVE</b>To compare and analyze the data of breast cancer recurrence after breast-conserving therapy (BCT), and to find high risk factors that can affect local recurrence.</p><p><b>METHODS</b>A total of 1034 patients in the data base between January 2000 and June 2008 were analyzed retrospectively. The patients aged 23 to 94 years when diagnosed (median age, 48 years). The data was investigated to compare of two different groups in local recurrence rate and survival rate. The high risk factors of recurrence after BCT [estrogen receptor (ER)/progesterone receptor (PR), human epidermal growth factor receptor (HER-2), age, lymph node involvement, tumor diameter, neoadjuvant chemotherapy, pathological status] were studied.</p><p><b>RESULTS</b>The patients were followed-up to June 2010, and the median period was 42 months (range, 3-126 months). During the period, 35 patients developed ipsilateral breast tumor recurrence (3.3%), 47 patients had metastasis to distant organs (4.5%). The 5-year disease-free survival was 87.7%, 5-year distant disease-free survival was 94.0%. The lymph node status, HER-2 status and age were significant risk factors for ipsilateral breast tumor recurrence on univariate analysis. One peak recurrence period was from the 2nd to 3rd year, and the other was from the 5th to 6th year after the operation. The HER-2 status was independent factors of ipsilateral breast tumor recurrence on multivariate analysis.</p><p><b>CONCLUSIONS</b>The recurrence happens primarily in the 2nd to 3rd and the 5th to 6th year after the breast-conserving therapy. HER-2 status is an independent factor of ipsilateral breast tumor recurrence. The patients with high risk factors of recurrence should be treated more aggressively.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Breast Neoplasms , General Surgery , Female , Follow-Up Studies , Humans , Kaplan-Meier Estimate , Mastectomy, Segmental , Middle Aged , Neoplasm Recurrence, Local , Prognosis , Proportional Hazards Models , Receptor, ErbB-2 , Metabolism , Retrospective Studies , Risk Factors , Young Adult
19.
Chinese Medical Journal ; (24): 2847-2852, 2010.
Article in English | WPRIM | ID: wpr-237403

ABSTRACT

<p><b>BACKGROUND</b>Quadratus femoris pedicled bone grafting has yielded satisfactory long-term clinical outcome for osteonecrosis of the femoral head (ONFH) in pre-collapse ONFH without extensive lesion. However, for pre-collapse ONFH with extensive necrotic area, it is still challenging to preserve the femoral head. The current study aimed to introduce a new technique of deliquesce strut with titanium mesh containing bone grafting pedicled with the femoral quadratus and to evaluate its short-term outcomes.</p><p><b>METHODS</b>From January 2008 to December 2008, 10 ONFH patients (12 hips) underwent operations by a new technique of deliquesce strut with titanium mesh containing bone grafting pedicled with the femoral quadratus (group A). According to the ARCO classification system, there were two hips in stage II B and 10 hips in stage II C. Also in the same period, 12 ONFH patients (16 hips) underwent operations by the conventional procedure of quadratus femoris pedicled bone grafting (group B). There were 6 hips in stage II B and 10 hips in stage II C. All patients were males and suffered from alcohol induced ONFH. For the new technique, the necrotic area was evaluated, and a titanium mesh piece of the same size (range from 2.5 cm × 2.8 cm to 2.8 cm × 3.4 cm) was obtained and shaped to match the contour of the head. The cancellous bone was first placed underneath the subchondral bone and was densely impacted (about 1 to 2 mm thick). Then the titanium mesh piece was inserted. The length of the decompressive trough was measured. A titanium cylinder mesh cage with a diameter of 1.6 cm of the same length was obtained, with a "U" shaped window in the wall being created to make room for the muscle pedicle. The muscle pedicle bone was inserted into the titanium mesh cage to form a bone graft-titanium cage complex and, then the complex was inserted. The hundred percent score method was used for outcome evaluation. Clinical and radiographic outcomes were compared between group A and group B.</p><p><b>RESULTS</b>The average operative time was 150 minutes (130 to 185 minutes) in group A, with an average of 130 minutes (120 to 180 minutes) in group B. The mean blood loss was 400 ml (300 to 500 ml) in group A and 350 ml (250 to 500 ml) in group B. Group A patients were followed up for an average of 19.2 months (14 to 24 months), with an average of 18.5 months (12 to 24 months) for Group B. Full weight bearing was allowed 5 to 7 months postoperatively. Pain and function were obviously improved. For group A, pain score improved from a mean of 9.8 points preoperatively to an average of 24.6 points postoperatively, and function score improved from a mean of 9.0 points preoperatively to an average of 17.4 points postoperatively. In group B, pain score improved from a mean of 9.5 points preoperatively to an average of 24.2 points postoperatively and function score improved from a mean of 9.2 points preoperatively to an average of 17.2 points postoperatively. The range of motion changed the least, with score improvement from a preoperative mean of 13.9 points to postoperative 16.8 points for group A and from a preoperative mean of 13.7 points to postoperative 16.5 points for group B. Radiographic score improved from preoperative 31 points to postoperative 38 points for group A, in comparison with an improvement from preoperative 31 points to postoperative 37 points for group B. At the latest follow up, 11 hips were rated as excellent and 1 hip was better for group A, with 14 hips being rated as excellent and 2 hips being better in group B. There was no statistically significant difference between groups A and B in clinical and radiographic outcomes.</p><p><b>CONCLUSION</b>For ONFH in stage ARCO IIC, satisfactory clinical outcome can be achieved by the new technique in the short-term period while the long-term clinical outcome has yet to be determined.</p>


Subject(s)
Adult , Bone Transplantation , Methods , Ethanol , Femur , General Surgery , Femur Head Necrosis , General Surgery , Humans , Male , Range of Motion, Articular , Surgical Mesh , Titanium
20.
Chinese Journal of Oncology ; (12): 511-514, 2010.
Article in Chinese | WPRIM | ID: wpr-260364

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the prognostic significance of Her-2 expression in node-positive and node-negative breast cancer in Chinese women.</p><p><b>METHODS</b>The Her-2 expression in breast cancers from 981 patients was detected by immunohistochemistry with anti-Her-2 (CB11) monoclonal antibody. The survival curves were analyzed by Kaplan-Meier method, and Cox regression model was applied to determine whether this factor is an independent predictor of survival in multivariate analysis.</p><p><b>RESULTS</b>Nineteen point seven percent of the patients showed positive Her-2 expression in their tumors. Patients with Her-2-positive tumors tended to be younger. The high level Her-2 expression was significantly associated with negative estrogen receptor and progesterone receptor status in their tumors (P < 0.05). Among 387 patients with node-positive disease, the 5-year disease-free survival (DFS) rate and 5-year overall survival (OS) rate were significantly lower in patients with Her-2-positive tumors than in patients with Her-2-negative tumors (DFS: 48.8% vs. 66.9%, P = 0.009; OS: 55.2% vs. 76.4%, P = 0.001), and Her-2 expression was an independent unfavorable prognostic factor for OS, but not for DFS in patients with node-positive disease. Among 591 patients with node-negative disease, Her-2 expression was not significantly associated with DFS and OS (P > 0.05).</p><p><b>CONCLUSION</b>Her-2 expression is an important prognostic factor in patients with node-positive disease, but not for patients with node-negative disease in Chinese women.</p>


Subject(s)
Adult , Age Factors , Aged , Aged, 80 and over , Breast Neoplasms , Metabolism , Pathology , Disease-Free Survival , Female , Follow-Up Studies , Gene Expression Regulation, Neoplastic , Humans , Lymph Nodes , Pathology , Lymphatic Metastasis , Middle Aged , Neoplasm Staging , Proportional Hazards Models , Receptor, ErbB-2 , Metabolism , Receptors, Progesterone , Metabolism , Survival Rate
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