Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 33
Filter
Add filters








Year range
1.
Chinese Medical Journal ; (24): 944-953, 2021.
Article in English | WPRIM | ID: wpr-878119

ABSTRACT

BACKGROUND@#Coronavirus disease 2019 (COVID-19), which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has rapidly spread throughout the world. In this study, we aimed to identify the risk factors for severe COVID-19 to improve treatment guidelines.@*METHODS@#A multicenter, cross-sectional study was conducted on 313 patients hospitalized with COVID-19. Patients were classified into two groups based on disease severity (nonsevere and severe) according to initial clinical presentation. Laboratory test results and epidemiological and clinical characteristics were analyzed using descriptive statistics. Univariate and multivariate logistic regression models were used to detect potential risk factors associated with severe COVID-19.@*RESULTS@#A total of 289 patients (197 nonsevere and 92 severe cases) with a median age of 45.0 (33.0, 61.0) years were included in this study, and 53.3% (154/289) were male. Fever (192/286, 67.1%) and cough (170/289, 58.8%) were commonly observed, followed by sore throat (49/289, 17.0%). Multivariate logistic regression analysis suggested that patients who were aged ≥ 65 years (OR: 2.725, 95% confidence interval [CI]: 1.317-5.636; P = 0.007), were male (OR: 1.878, 95% CI: 1.002-3.520, P = 0.049), had comorbid diabetes (OR: 3.314, 95% CI: 1.126-9.758, P = 0.030), cough (OR: 3.427, 95% CI: 1.752-6.706, P < 0.001), and/or diarrhea (OR: 2.629, 95% CI: 1.109-6.231, P = 0.028) on admission had a higher risk of severe disease. Moreover, stratification analysis indicated that male patients with diabetes were more likely to have severe COVID-19 (71.4% vs. 28.6%, χ2 = 8.183, P = 0.004).@*CONCLUSIONS@#The clinical characteristics of those with severe and nonsevere COVID-19 were significantly different. The elderly, male patients with COVID-19, diabetes, and presenting with cough and/or diarrhea on admission may require close monitoring to prevent deterioration.


Subject(s)
Adult , COVID-19/pathology , China/epidemiology , Comorbidity , Cough , Cross-Sectional Studies , Diarrhea , Female , Humans , Male , Middle Aged , Retrospective Studies , Risk Factors
2.
Article in Chinese | WPRIM | ID: wpr-746219

ABSTRACT

Objective To obverse the changes ofmacular choroidal thickness (CT) in patients with mild to moderate Alzheimer's disease (AD).Methods This was a case-control study.Twenty-one patients with mild to moderate AD confirmed by Neurology Department of Jinhua Central Hospital from November 2016 to June 2018 and 21 age-matched control subjects were concluded in the study.There was no significant difference in age (t=0.128),intraocular pressure (t=0.440) and axial length (t=1.202) between the two groups (P>0.05).There was significant difference in mini-mental state examination score (t=8.608,P<0.05).CT was measured by OCT with enhanced depth imaging technique in the subfoveal choroid,at 0.5 mm and 1.0 mm from the center of the fovea nasal (NCT0.5,1.0mm),temporal (TCT0.5,1.0mm),superior (SCT0.5,1.0mm),and inferior (ICT0.5,1.0mm).Independent-samples t test was used to compare the results obtained from these two groups.Results SFCT (t=2.431),NCT0.5,1.0 mm (t=3.341,2.640),TCT0.5,1.0 mm (t=3.340,2.899),SCT0.5.1.0 mm (t=3.576,3.751) and ICT0.5 (t=2.897,2.903) were significantly thinner in AD eyes than those in control eyes.Conclusion Compared with healthy subjects,patients with mild to moderate AD showed a significant reduction in CT.

3.
Article in Chinese | WPRIM | ID: wpr-779527

ABSTRACT

Objective To evaluate the effect of ambient temperature on the activity influenza like illness (ILI) and laboratory-confirmed influenza (LAB) in Wuxi City. Methods Daily data of meteorological, ILI and detected influenza virus from 31 December 2012 to 31 December 2017 were collected. Distributed lag non-linear model (DLNM) was used to evaluate the exposure-lag-response of ILI and LAB activity to daily ambient temperature.Results During the period, the overall ILI% was 4.96% and influenza detection positive rate was 12.28% in Wuxi city. The overall cumulative association analysis suggested non-linear relationship between ambient temperature and influenza: U-shaped for ILI, while L-shaped relationship for LAB. Low temperature (<10℃ ) had strong and longer delay effect than hightemperature (>20℃ ) for ILI. The cold effect for LAB was stronger and longer delay,and the low temperature (<10℃ ) was risk factor for LAB. Conclusions The ambient temperature significant correlates with ILI and LAB, and low temperature might be risk factor with lag effect.

4.
Acta Physiologica Sinica ; (6): 279-286, 2019.
Article in Chinese | WPRIM | ID: wpr-777188

ABSTRACT

The aim of this study was to investigate the role of S100 calcium binding protein A16 (S100A16) in lipid metabolism in hepatocytes and its possible biological mechanism. HepG2 cells (human hepatoma cell line) were cultured with fatty acid to establish fatty acid culture model. The control model was cultured without fatty acid. Each model was divided into three groups and transfected with S100a16 over-expression, shRNA and vector plasmids, respectively. The concentration of triglyceride (TG) in the cells was measured by kit, and the lipid droplets was observed by oil red O staining. Immunoprecipitation and mass spectrometry were used to find the interesting proteins interacting with S100A16, and the interaction was verified by immunoprecipitation. The further mechanism was studied by Western blot and qRT-PCR. The results showed that the intracellular lipid droplet and TG concentrations in the fatty acid culture model were significantly higher than those in the control model. The accumulation of intracellular fat in the S100a16 over-expression group was significantly higher than that in the vector plasmid transfection group. There was an interaction between heat shock protein A5 (HSPA5) and S100A16. Over-expression of S100A16 up-regulated protein expression levels of HSPA5, inositol-requiring enzyme 1α (IRE1α) and pIREα1, which belong to endoplasmic reticulum stress HSPA5/IRE1α-XBP1 pathway. Meanwhile, over-expression of S100A16 up-regulated the mRNA expression levels of adipose synthesis-related gene Srebp1c, Acc and Fas. In the S100a16 shRNA plasmid transfection group, the above-mentioned protein and mRNA levels were lower than those of vector plasmid transfection group. These results suggest that S100A16 may promote lipid synthesis in HepG2 cells through endoplasmic reticulum stress HSPA5/IRE1α-XBP1 pathway.


Subject(s)
Endoplasmic Reticulum Stress , Endoribonucleases , Physiology , Heat-Shock Proteins , Physiology , Hep G2 Cells , Humans , Lipid Metabolism , Protein-Serine-Threonine Kinases , Physiology , S100 Proteins , Physiology , Triglycerides , X-Box Binding Protein 1 , Physiology
5.
Article in Chinese | WPRIM | ID: wpr-804957

ABSTRACT

Objective@#To investigate the epidemiological characteristics and causes of an outbreak of respiratory infection in schools.@*Methods@#Epidemiological investigation was carried out on respiratory infection cases in schools. Pharyngeal swab samples of children were collected for respiratory tract virus nucleic acid detection, and hexon genes were amplified. Positive PCR result were sequenced.@*Results@#All cases were in school, involving 2 classes, 12 cases in class 2 (3) and 13 cases in class 3 (4), all on the west side of the 2nd and 3rd floors of teaching building 2. From September 3 to September 12, a total of 25 cases were involved, with the onset peak on the 10th and 11th. The clinical features were fever, cough, sore throat and aching limbs, etc. The onset was aggregated, the course of the disease was about 5 d, the prognosis was good, and there were no deaths. There are 13 males and 12 females. The males to females ratio is 1.08∶1. The age distribution is 7-9 years, with an average of 8.6 years. The result of fluorescence quantitative PCR were negative for influenza A/B virus and positive for adenovirus. The products of positive samples after PCR amplification were sequenced as AdV-3 and AdV-4.@*Conclusions@#The combined result of epidemiological investigation and laboratory tests demonstrated that this epidemic of school respiratory tract infection is an upper respiratory tract infection caused by adenovirus B3 and E4 mixed infection.

6.
Article in Chinese | WPRIM | ID: wpr-690476

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the regulatory role of classical Shh signaling pathway in the development of the epithelium and mesenchyme (bronchial cartilage and smooth muscles) during lung development in fetal mice.</p><p><b>METHODS</b>Immunohistochemical technique was used to detect the expression of Shh signaling pathway receptor Smo and Pdgfr-α in murine fetal lungs to explore the spatial and temporal characteristics of their expression. Based on the interstitial specificity of Pdgfr-α expression, we constructed a Pdgfr-α-cre to establish a E12.5 - E16.5 transgenic mice with specific knockout of the key Shh signaling molecule Smo in the pulmonary interstitium with tamoxifen induction. Immunofluorescence technique was used to observe the epithelium and mesenchyme (bronchial cartilage and smooth muscle) during fetal lung development in the transgenic mice to assess the role of Shh signaling pathway in the epithelial-to-mesenchymal (EMT) transition during the lung development.</p><p><b>RESULTS</b>Smo was highly expressed in the epithelial and stromal lung tissues in the pseudoglandular stage and was gradually lowered over time with its distribution mainly in the interstitial tissues. Pdgfr-α was enriched in the distal lung epithelial and mesenchy tissues in early embryonic lungs and gradually migrated to the proximal stroma until becoming concentrated around the main bronchial proximal stroma. We successfully specific established mouse models of specific mesenchymal Smo knockout. Compared with the control group, the transgenic mice during E12.5-E16.5 showed significantly reduced lung the volume and bronchial branching with also decreased expression of the proximal epithelial P63 (P<0.05). The transgenic mice exhibited alterations in the expression of α-smooth muscle actin with delayed bronchial cartilage development and decreased expression of mucoprotein.</p><p><b>CONCLUSION</b>The temporospatial specific expression of Shh signaling pathway plays an important role in developmental regulation of mouse embryonic lung epithelium and mesenchyme (bronchial cartilage and smooth muscle).</p>

7.
China Journal of Endoscopy ; (12): 37-41, 2017.
Article in Chinese | WPRIM | ID: wpr-661150

ABSTRACT

Objective To explore the clinical significance of Nano-Carbon particles and 3D laparoscopy in central compartment lymph node dissection and parathyroid glands protection in treatment of cN0 thyroid cancer. Methods We conduct a retrospective analysis of sixty-five patients with cN0 thyroid cancer who were received 3D laparoscopic thyroidectomy in the last 3 years. All patients were received total resection of thyroid plus the affected side and (or) contralateral side central compartment lymph node dissection. All patients were allocated to control group (n = 32) and carbon nano-particles trace group (tracer group, n = 33). The lymph node-related indexes (including number of dissected lymph node at Ⅵ area, number of Metastatic lymph node and Frozen lymph node-positive rate at Ⅵ area), serum calcium (24 h after surgery) and PTH (48 h after surgery) were collected and compared between the 2 groups. Results Number of dissected lymph node at Ⅵ area, positive rates of intraoperative frozen-section examination of parathyroid glands and PTH (48 h after surgery) were found statistical higher in nanoparticles group than control (P < 0.05). No statistical difference were found in Number of Metastatic lymph node and serum calcium (24 h after surgery) (P < 0.05). Conclusion The clinical significance of carbon nanoparticles and 3D laparoscopy is effective and feasible for central compartment lymph node dissection and parathyroid glands protection in treatment of cN0 thyroid cancer.

8.
Article in Chinese | WPRIM | ID: wpr-660044

ABSTRACT

Objeetive To investigate the detection of hemodynamiCS parameters of conjunctival microvessels by functional slit lamp biomicroscopy (FSLB) in normal adult and its clinical application values.Methods Randomly selected 16 postgraduate students aged 21 to 25 years from the Nanchang University and Zhongshan Ophthalmic Center,including 8 males and 8 females.The density,length and diameter of conjunctival microvessel and the flow velocity of arteriolar and venular blood were measured by FSLB and software program (BenoitTM,TruSoft Inc,St.Petersburg,FL,USA),as well as the indicators' characteristics of the subjects' were analyzed.Results As for subjects' right eyes,the microvascular density,length,diameter,and the arteriolar and venular blood flow velocity were no significant difference between the upper,and lower sides,as well as between nasal and temporal sides (all P > 0.05);meanwhile the microvascular density,length and diameter of the upper and lower sides were both lower than those of the nasal and temporal sides,and the differences were statistically significant (all P < 0.05),while the arteriolar and venular blood flow velocity of the upper and lower sides were both significantly higher than those of the nasal and temporal sides,and the differences were statistically significant (all P < 0.05).In addition,the changes in hemodynamics parameters of conjunctival microvessels of the left eyes and total eyes in subjects were consistent with the right eyes.Conclusion FSLB can clearly obtain hemodynamics parameters of conjunctival microvessels in healthy grown-ups,and has a promising clinical value.

9.
China Journal of Endoscopy ; (12): 37-41, 2017.
Article in Chinese | WPRIM | ID: wpr-658264

ABSTRACT

Objective To explore the clinical significance of Nano-Carbon particles and 3D laparoscopy in central compartment lymph node dissection and parathyroid glands protection in treatment of cN0 thyroid cancer. Methods We conduct a retrospective analysis of sixty-five patients with cN0 thyroid cancer who were received 3D laparoscopic thyroidectomy in the last 3 years. All patients were received total resection of thyroid plus the affected side and (or) contralateral side central compartment lymph node dissection. All patients were allocated to control group (n = 32) and carbon nano-particles trace group (tracer group, n = 33). The lymph node-related indexes (including number of dissected lymph node at Ⅵ area, number of Metastatic lymph node and Frozen lymph node-positive rate at Ⅵ area), serum calcium (24 h after surgery) and PTH (48 h after surgery) were collected and compared between the 2 groups. Results Number of dissected lymph node at Ⅵ area, positive rates of intraoperative frozen-section examination of parathyroid glands and PTH (48 h after surgery) were found statistical higher in nanoparticles group than control (P < 0.05). No statistical difference were found in Number of Metastatic lymph node and serum calcium (24 h after surgery) (P < 0.05). Conclusion The clinical significance of carbon nanoparticles and 3D laparoscopy is effective and feasible for central compartment lymph node dissection and parathyroid glands protection in treatment of cN0 thyroid cancer.

10.
Article in Chinese | WPRIM | ID: wpr-657700

ABSTRACT

Objeetive To investigate the detection of hemodynamiCS parameters of conjunctival microvessels by functional slit lamp biomicroscopy (FSLB) in normal adult and its clinical application values.Methods Randomly selected 16 postgraduate students aged 21 to 25 years from the Nanchang University and Zhongshan Ophthalmic Center,including 8 males and 8 females.The density,length and diameter of conjunctival microvessel and the flow velocity of arteriolar and venular blood were measured by FSLB and software program (BenoitTM,TruSoft Inc,St.Petersburg,FL,USA),as well as the indicators' characteristics of the subjects' were analyzed.Results As for subjects' right eyes,the microvascular density,length,diameter,and the arteriolar and venular blood flow velocity were no significant difference between the upper,and lower sides,as well as between nasal and temporal sides (all P > 0.05);meanwhile the microvascular density,length and diameter of the upper and lower sides were both lower than those of the nasal and temporal sides,and the differences were statistically significant (all P < 0.05),while the arteriolar and venular blood flow velocity of the upper and lower sides were both significantly higher than those of the nasal and temporal sides,and the differences were statistically significant (all P < 0.05).In addition,the changes in hemodynamics parameters of conjunctival microvessels of the left eyes and total eyes in subjects were consistent with the right eyes.Conclusion FSLB can clearly obtain hemodynamics parameters of conjunctival microvessels in healthy grown-ups,and has a promising clinical value.

11.
Article in Chinese | WPRIM | ID: wpr-789424

ABSTRACT

Objective To explore the therapeutic effect of group medical visits on high-risk elder people suffering senile osteoporosis.Methods A total of 86 high-risk elder people aged over 65, suffering senile osteoporosis and with the OSTA index below-4 were randomly selected, and were separated into an intervention group and a control group, with 43 patients belonging to each group.The dual-energy X-ray absorptiometry(DXA) was used to measure the left femoral neck bone mineral density (LFN BMD), and relevant biochemical indexes thereof and biochemical indicators of bone metabolism thereof were measured at the same time.The osteoporotic knowledge test(OKT) and the osteoporotic self-efficiency scheme(OSES) were used to make relevant evaluations.Group interventions included the following four items: health edutaion and individual technical guidance, state of illness monitoring, group activities and prescription of medicines.At one year after the intervention, the above-mentioned items were to be evaluated again for the two groups.Results After the intervention, the blood calcium(Ca2+), phosphorus(P3+) and LFN BMD of the two groups had no statistical difference(P>0.05);the OKT and OSES scores as well as the level of 25(OH)VitD3 of the intervention group were all higher than those of the control group as well as those of the intervention group before the intervention(P<0.05).After intervention, the PTH, PINP and β-CTX levels of the intervention group were lower than those of the control group as well as those of the intervention group before the intervention(P<0.05).Conclusion The group medical visit is an effective community-based management intervention mode suitable for high-risk patients suffering osteoporosis.

12.
Article in Chinese | WPRIM | ID: wpr-616691

ABSTRACT

Optical coherence tomography (OCT) has played a revolutionary role in the development of ophthalmology.Since the 1990s,it has been constantly improved in terms of speed,resolution and sensitivity.The technique has also seen a variety of extensions aiming to assess ftmctional aspects of the tissue.Doppler combined with OCT technology (DOCT) can provide 3D vascular images,also referred to as the OCT angiography.DOCT can achieve visualization,quantitative blood flow,scanning of layer retinal blood vessels,and the scanning speed is fast.It has depth resolution,clear image.As a new noninvasive optical imaging technique,DOCT can realize retinal vascular network images and provide meaningful image data.It is a major breakthrough on the eye detection methods and helpful in diagnosis,monitoring for the eye disease in the future.This article reviews the application progress of DOCT in eye diseases.

13.
Chinese Medical Journal ; (24): 181-186, 2016.
Article in English | WPRIM | ID: wpr-310686

ABSTRACT

<p><b>BACKGROUND</b>It is important to achieve the definitive pathogen identification in hospital-acquired pneumonia (HAP), but the traditional culture results always delay the target antibiotic therapy. We assessed the method called quantitative loop-mediated isothermal amplification (qLAMP) as a new implement for steering of the antibiotic decision-making in HAP.</p><p><b>METHODS</b>Totally, 76 respiratory tract aspiration samples were prospectively collected from 60 HAP patients. DNA was isolated from these samples. Specific DNA fragments for identifying 11 pneumonia-related bacteria were amplified by qLAMP assay. Culture results of these patients were compared with the qLAMP results. Clinical data and treatment strategies were analyzed to evaluate the effects of qLAMP results on clinical data. McNemar test and Fisher's exact test were used for statistical analysis.</p><p><b>RESULTS</b>The detection of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Stenotrophomonas maltophilia, Streptococcus pneumonia, and Acinetobacter baumannii by qLAMP was consistent with sputum culture (P > 0.05). The qLAMP results of 4 samples for Haemophilus influenzae, Legionella pneumophila, or Mycoplasma pneumonia (MP) were inconsistent with culture results; however, clinical data revealed that the qLAMP results were all reliable except 1 MP positive sample due to the lack of specific species identified in the final diagnosis. The improvement of clinical condition was more significant (P < 0.001) in patients with pathogen target-driven therapy based on qLAMP results than those with empirical therapy.</p><p><b>CONCLUSION</b>qLAMP is a more promising method for detection of pathogens in an early, rapid, sensitive, and specific manner than culture.</p>


Subject(s)
Anti-Bacterial Agents , Therapeutic Uses , Cross Infection , Drug Therapy , Microbiology , Pilot Projects , Pneumonia , Drug Therapy , Microbiology , Prospective Studies , Pseudomonas aeruginosa , Virulence
14.
Article in Chinese | WPRIM | ID: wpr-486913

ABSTRACT

Objective To investigate the epidemiology of respiratory viruses in children from Wuxi area.Methods A total of 2 747 cases of children diagnosed with acute respiratory infection in Wuxi during 2011 —2014 were collected.Reverse transcription-polymerase chain reaction was used to detect nine kinds of respiratory viruses,including influenza virus A (Flu A),influenza virus B (Flu B),parainfluenza virus (PIV)Ⅰ-Ⅳ,adenovirus (ADV),respiratory sycytial virus (RSV),human metaneumovirus (hMPV), human bocavirus (HBov),human coronaviruses (hCov)and human rhinovirus (HRV).The categorical data were compared using chi square test.Results A total of 856 among the 2 747 samples were tested positive for respiratory virus nucleic acid,with the positive rate of 31 .16%.The viral distribution was uneven in different seasons,and the infection peaked in winter and spring.The virus detection rate was highest in age 1 to 2 year group (up to 40.18%),and followed by age 6 to 12 year group (32.63%).Flu A virus was the most frequently detected virus,accounting for 7.54% (207/2 747);followed by PIV, accounting for 6.95 % (191/2 747);and Flu B accounted for 4.22%(116/2 747).There were 84 cases of mixed infection of two or more kinds of respiratory viruses,with positive rate of 3.06% (84/2 747 ). Conclusions Our study suggests that Flu A is the most common pathogen in children with acute respiratory infections in Wuxi area during 2011 —2014;virus detection rate is highest in age 1 to 2 year group;and parainfluenza virus is almost detected throughout the year,while the rest of respiratory viruses are commonly seen in winter and spring.

15.
The Journal of Practical Medicine ; (24): 1075-1078, 2015.
Article in Chinese | WPRIM | ID: wpr-464418

ABSTRACT

Objective To construct 1.3-fold-overlength infectious clone of hepatitis B virus isolated from Chinese patients , observe the expression of plasmid in Huh7 of liver cancer cells and establish genome of HBV in vitro. Methods HBV DNA in serum was extracted from HBV patient. SOE-PCR was performed to produce a 1.3-fold-overlength genome of HBV. The plasmid was named pHBV1.3 (C). After that,pHBV1.3 (C) was transfected into Huh7 cells, HBV related viral antigens and DNA were detected by ELISA,Western Blot and Fluorescence quantitative PCR. Furthermore, adefovir dipivoxil, a clinic anti-viral drug, was utilized to test the sensitivity of the new infectious clone. Results An infectious clone of pHBV1.3 (C) was successfully constructed. HBV gene carried in pHBV1.3 (C) could be efficiently replicated and expressed in Huh7 cells. Adefovir could inhibit HBV replication in this HBV cell model. Conclusions A recombinant plasmid containing 1.3-fold-overlength of HBV genotype C was successfully constructed. This construct is competent to support viral transcription and replication in vitro , suggesting that infectious cells are expected to be a new model of HBV infection in vitro.

16.
Article in English | WPRIM | ID: wpr-820397

ABSTRACT

OBJECTIVE@#To evaluate the efficacy and safety of rhPTH (1-34) vs. elcatonin.@*METHODS@#Sixty patients with primary OP were randomly divided into two groups according to the ratio of 3:1. rhPTH (1-34) group (PTH group) was treated with subcutaneous injection of rhPTH (1-34) 20 μ g daily for 18 months, and the elcatonin group (CT group) was treated with intramuscular injection of elcatonin 20 U weekly for 12 months. Bone mineral density (BMD) of the lumbar spine 2-4 (L2-4) and femoral neck, serum calcium and phosphorus, urinary calcium, serum bone specific alkaline phosphatase (BSAP), and urinary c-terminal telopeptides of type I collagen/creatinine (uCTX-I/Cr) were tested at baseline, and 6, 12, and 18 months after treatment.@*RESULTS@#In PTH group, BMD of L2-4 at 6, 12, and 18 months, BDM of femoral neck at 18 month, BSAP at 6 and 12 months and uCTX- I/Cr at 6, 12 and 18 months were all significantly raised. In CT group, BMD of L2-4 at 12 month and that of femoral neck at 12 and 18 months were significantly elevated, while BSAP was significantly decreased at 12 and 18 months, and no significant difference on CTX- I/Cr was observed. When BMD growth and growth rate between two groups were compared, PTH group had better improvement in L2-4 BMD and growth rate than CT group at 6, 12, and 18 months. BMD growth and growth rate of femoral neck at 12 month and its growth at 18 month in CT group were higher than in PTH group, but there was no significant difference between two groups regarding the growth rates at 18 month. Besides, there were no significant differences regarding the rates of adverse reactions between two groups.@*CONCLUSIONS@#rhPTH (1-34), is safe and effective in the treatment of primary OP. It is superior to elcatonin in improving vertebral BMD at onset time, growth rate and growth range, but inferior to elcatonin at BMD of femoral neck.

17.
Article in Chinese | WPRIM | ID: wpr-264937

ABSTRACT

This study was purposed to investigate the clinical value of HLA matching(low and high resolution) and its effect on outcome of the patients received umbilical cord blood transplantation(UCBT). Sequence-specific oligonucleotide probe (SSOP) , sequence-based typing (SBT) and sequence-specific primers(SSP) were used to perform high resolution HLA matching for HLA-A, -B, -Cw, -DRB1, -DQB1 and low resolution for HLA-A, B, DRB1 among 34 patients with hematologic malignancies who received unrelated UCB transplantation and grafts. The effects of HLA matching (low or high resolution ) on leading engraftment, hematopoietic reconstitution, graft-versus-host disease (GVHD) and infection after UCB transplantation were analyzed by comparison. The results showed that the median of total nucleated cells (TNC) of transplanted cord blood was 6.0×10(7)/kg, The time of neutrophil recovery was significantly shortened when more than 5×10(7)/kg TNC were transplanted (P < 0.05). The HLA-(6-10)/10 group of high resolution HLA matching was better than the HLA (4-5)/10 group in the respect of leading engraftment, the time of platelet recovery and the rate of acute GVHD (P < 0.05). In contrast, HLA-I+II locus, HLA-DRB1 or HLA-DQB1 locus mismatch could prolong the platelet engraftment time (P < 0.05). There was statistical difference in the time of platelet recovery, the rate of acute GVHD between the HLA (5-6)/6 group of low resolution HLA matching and the HLA (3-4)/6 group after UCB transplantation (P < 0.05), but the mismatch locus of HLA with low resolution did not correlate with the time of platelet recovery (P > 0.05). It is concluded that the high resolution HLA matching between patients received unrelated UCB transplantation and grafts may contribute to select the better UCB, that has important clinical value to promote hematopoietic reconstitution and to reduce the complications after UCB transplantation.


Subject(s)
Adolescent , Adult , Child , Cord Blood Stem Cell Transplantation , Methods , Female , Hematologic Neoplasms , Therapeutics , Histocompatibility Testing , Methods , Humans , Male , Young Adult
18.
Chinese Journal of Hematology ; (12): 1038-1043, 2013.
Article in Chinese | WPRIM | ID: wpr-295745

ABSTRACT

<p><b>OBJECTIVE</b>To find out the distributed characteristics of KIR2DL1 alleles frequencies and the recognition HLA-C ligand in the Chinese Han population.</p><p><b>METHODS</b>The 111 patients and 116 donors from CMDP were performed the KIR2DL1 high-resolution typing and KIR genotyping using sequence-based testing (SBT) and PCR-SSP methods.</p><p><b>RESULTS</b>A total of 224 individuals with KIR2DL1 locus was predominantly observed and accounted for 98.68% (224/227). There were 3 different KIR2DL1 alleles, including KIR2DL1*00302, *00201 and *00401 alleles polymorphism. The most common phenotype observed were KIR2DL1*00302 (84.82%, 380/448), KIR2DL1*00201 (12.05%, 54/448) and KIR2DL1*00401(3.13%,14/448), present at allele genotype frequencies of 61.04%,6.22% and 1.58% respectively. The allele homozygotic types of KIR2DL1*00302 and KIR2DL1*00302 were the most frequent in 6 KIR2DL1 allele by high resolution typing. The allele heterozygous types of KIR2DL1*00302 and KIR2DL1*00401 presented statistically different in haplotypes A/A and B/x (P=0.001), and KIR2DL1*00401 lacked of all A/A haplotype. The KIR2DL1*00302 and KIR2DL1*00201 allele had significant positive associations between different KIR pairs of KIR2DS1, KIR2DL3, KIR2DS4 and KIR3DL1/S1 using linkage disequilibrium analysis (P<0.01), respectively. In the receptor-ligand of KIR/HLA model after allo-HSCT, KIR2DL1*00302 alleles correlated with their HLA-C2 group ligands. KIR2DL1*00302 and HLA-C*06:02 was the most common combination ligand model, but KIR2DL1*00302 and HLA-C*01:02 was the most frequent mismatch ligand model with the development of NK cell-induced alloreactivity, meanwhile there was statistically significant difference of frequency distribution (P<0.05).</p><p><b>CONCLUSION</b>The KIR2DL1*00302 was the most frequent allele in Chinese Han population. The KIR2DL1 high resolution typing would be beneficial for predicting donor NK cells all activity after hematopoietic stem cell transplantation and selecting suitable donors.</p>


Subject(s)
Alleles , Asian Continental Ancestry Group , Genetics , Gene Frequency , Genotype , HLA-C Antigens , Genetics , Haplotypes , Histocompatibility Testing , Humans , Ligands , Polymorphism, Genetic , Receptors, KIR2DL1 , Genetics
19.
Article in Chinese | WPRIM | ID: wpr-316605

ABSTRACT

<p><b>OBJECTIVE</b>To study the therapeutic effect of photocatalytic nano-TiO₂ on nasopharyngeal carcinoma xenograft in nude mice and underlying mechanism.</p><p><b>METHODS</b>Nude mice bearing human nasopharyngeal carcinoma xenograft were randomly divided into six groups: nano-TiO₂ + UV irradiation (with gradient concentration of nano-TiO₂); nano-TiO₂ alone and UV irradiation alone and blank control. The nano-TiO₂ suspension was injected into xenografts, and 24 h after UV light with the wave length of 330 - 400 nm, all the xenografts were removed and sectioned for HE staining. Ultrastructure and apoptosis of tumor cells in the xenografts were observed by transmission electron microscope (TEM). The expression of Caspase-3 was examined immunohistochemical staining and the apoptosis was detected with TUNEL.</p><p><b>RESULTS</b>Pathological analysis showed significant inflammatory responses (grade II and III) with local necrosis occurred in tumor tissues after nano-TiO₂ photodynamic therapy, but not in the negative control and blank control. TEM showed the nano-TiO₂ particles entered into the cytoplasm and the nucleus of tumor cells and many tumor cells had morphological changes for apoptosis. Significant positive expression of Caspase-3 and TUNEL-positive cells were found in the the xenografts with the treatments of nano-TiO₂ + UV irradiation compared to control (P < 0.01), which were enhanced with the increases in nano-TiO₂ concentration (P < 0.01).</p><p><b>CONCLUSION</b>Photocatalytic nano-TiO₂ can inhibit the growth of nasopharyngeal carcinoma xenograft in nude mice by inducing Caspase-3 expression and apoptosis in the tumor cells.</p>


Subject(s)
Animals , Carcinoma , Caspase 3 , Metabolism , Cell Line, Tumor , Humans , Mice , Mice, Nude , Nasopharyngeal Neoplasms , Drug Therapy , Photochemotherapy , Titanium , Therapeutic Uses , Transfection , Xenograft Model Antitumor Assays
20.
Chinese Journal of Hematology ; (12): 353-357, 2012.
Article in Chinese | WPRIM | ID: wpr-359485

ABSTRACT

<p><b>OBJECTIVE</b>To study the impact of various human leukocyte antigen (HLA) high resolution typing mismatching of donor-recipient pairs on prognosis of unrelated donor hematopoietic stem cell transplantation.</p><p><b>METHODS</b>835 donor-recipient pairs of CMDP data from 2005 to 2010 were analyzed retrospectively. HLA-A, B, C, DRB1 and DQB1 typing were performed using SBT, SSOP and SSP methods. The diseases involved in acute myeloid leukemia (AML) (n = 288), acute lymphoid leukemia (ALL) (n = 227), chronic myeloid leukemia (CML) (n = 187), myelodysplastic syndrome (MDS) (n = 52), non-hodgkin's lymphoma(NHL) (n = 25), aplastic anemia(AA) (n = 42) and thalassemia (n = 14). Of 835 donor-recipient pairs, 362 were completely matched, 159 had a mismatch for a single allele, 125 had a mismatch for a single antigen, 95 had mismatched for both single allele and single antigen, 29 were mismatched at double allele, 20 at double antigen, 45 at multiple allele and antigen. The follow-up assessment was completed before March 2011.</p><p><b>RESULTS</b>HLA-matched pairs had higher overall survival (OS) than HLA-mismatched pairs (79.83% vs 73.15%), but there was no statistically significant differences (P > 0.05). HLA mismatch for a single allele plus a single antigen was a significantly risk factor for OS, disease free survival (DFS) and transplant-related mortality (TRM). The OS from high to low in different diseases were thalassemia, AA, CML, MDS, AML, NHL, and ALL. OS of HLA locus mismatch were DRB1 (94.4%), DQB1 (83.3%), B (75%), A (74.4%) and C (71.4%), respectively. OS of single allele mismatch at HLA locus from high to low were DRB1, C, A, B and DQB1.HLA-A, B, C locus mismatch were statistically significantly associated with lower OS and grade II-IV acute GVHD compared with HLA-matched pairs (P < 0.05). The donor-recipient pairs with HLA-B*15:01/B*15:05, DRB1*12:01/DRB1*12:02, C*04:01/C*03:04, DQB1*03:02/DQB1*03:03 alleles mismatch were given priority. But the donor-recipient pairs with HLA-B*39:01/B*39:05, C*15:02/C*14:02, C*08:01/C*03:04, C*07:02/C*15:02 alleles mismatch were risk factors for influence of OS and aGVHD.</p><p><b>CONCLUSION</b>The high resolution typing for HLA-A, B, C, DRB1, DQB1 can be identified nonpermissive mismatch, which is beneficial for the selection of a suitable donor improves survival on unrelated donor HSCT.</p>


Subject(s)
HLA Antigens , Genetics , Allergy and Immunology , Hematopoietic Stem Cell Transplantation , Histocompatibility Testing , Humans , Leukemia, Myeloid, Acute , Allergy and Immunology , General Surgery , Lymphoma, Non-Hodgkin , Allergy and Immunology , General Surgery , Myelodysplastic Syndromes , Allergy and Immunology , General Surgery , Prognosis , Retrospective Studies , Unrelated Donors
SELECTION OF CITATIONS
SEARCH DETAIL