Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 523
Filter
1.
International Eye Science ; (12): 298-303, 2022.
Article in Chinese | WPRIM | ID: wpr-913041

ABSTRACT

@#AIM: To investigate the status of astigmatism in children and adolescents aged 7-19 years in some areas of Xinjiang, and to analyze the distribution of astigmatism in children and adolescents in Xinjiang.METHODS: Cross-sectional study. This study took a purposive sampling survey of 41 schools in some areas of Xinjiang, including 20 primary schools and 21 middle schools, from May 2019 to December 2019. A total of 71 838 children and adolescents were included as the survey objects for relevant eye examinations.RESULTS: Among 71 838 students, 35 888 were found to have astigmatism(column ≤ -0.50D), and the astigmatism detection rate was 49.96%. The differences of astigmatism detection rate among different ages, genders, education levels, regions and ethnic groups were statistically significant(all <i>P</i><0.001). The detection rate of astigmatism increased with age from 7-16 years old, and reached the highest rate of 57.60% at 16 years old. The detection rate of astigmatism decreased from 16-19 years old, and the overall detection rate of astigmatism increased with age. With the increase of education level, the detection rate of astigmatism increases, and the detection rate of male astigmatism(51.25%)is higher than that of female astigmatism(48.62%). The detection rate of astigmatism in provincial capital cities(50.99%)is higher than that in non-provincial capital cities(48.05%); the detection rate of astigmatism in Han(53.41%)was higher than that in Uyghur(40.17%), Kazakhs(48.23%), Kyrgys(45.56%)and Hui(48.87%). The astigmatism type was mainly with-the-rule astigmatism(74.53%), and with the increase of age, the composition ratio of with-the-rule astigmatism decreased, while the composition ratio of against-the-rule astigmatism and oblique astigmatism increased. The final results of Logistic regression analysis showed that age, gender, nationality, region and education level were all influential factors of astigmatism.CONCLUSION: The prevalence of astigmatism in children and adolescents aged 7-19 years in some areas of Xinjiang is 49.96%, and the astigmatism is mainly with-the-rule astigmatism(74.53%). Age, gender, nationality, region and education level are the influential factors of astigmatism.

2.
Journal of Chinese Physician ; (12): 1214-1218, 2021.
Article in Chinese | WPRIM | ID: wpr-909691

ABSTRACT

Objective:To observe the efficacy of methimazole (MMI) combined with 1α-hydroxyvitamin D3 (alfacalcidol, ALF) in patients with Graves disease of high-titer thyrotropin receptor antibodies (TRAb) and to explore new clinical strategies to reduce serum TRAb in Graves disease.Methods:120 patients with Graves disease initially diagnosed in Quanzhou First Hospital Affiliated to Fujian Medical University and the People′s Hospital Affiliated to Quanzhou Medical College from June 2017 to June 2019 were prospectively selected as the research objects. All patients received conventional dose of MMI for anti hyperthyroidism treatment. The patients were randomly divided into three groups: group A [ n=40, treated with MMI combined with high-dose ALF (0.5 μg/d)], group B [ n=37, treated with MMI combined with low-dose ALF (0.25 μg/d)] and group C ( n=43, treated with MMI only). The treatment lasted for 24 weeks. The serum free triiodothyronine (FT 3), free thyroxine (FT 4), thyroid stimulating hormone (TSH) and TRAb in patients before and after above treatments were detected. The blood routine, liver function, alkaline phosphatase (ALP), 25(OH)D, serum calcium (CA) and serum phosphorus were detected regularly. Results:After drug treatment: ⑴ the thyroid function of the three groups returned to normal. The average daily dosage of MMI in group A was significantly lower than that in group B and C ( P<0.05), and that in group B was also lower than that in group C ( P<0.05), with significant difference. After 24 weeks of treatment, the daily dosage of MMI in group A and B was significantly lower than that in group C ( P<0.05). ⑵ There was no significant difference in thyroid function among the three groups. The concentration of serum TRAb in group A was significantly lower than that in group B and C ( P<0.05), and that in group B was also lower than that in group C ( P<0.05). ⑶ During the 24 week follow-up, there was no significant difference in serum 25(OH)D, ALP, Ca and P among the three groups ( P>0.05); no leukopenia in peripheral blood and no abnormal liver function were found in the three groups. Conclusions:MMI combined with ALF can effectively treat Graves′ disease, reduce the dosage of MMI drugs, decline the level of TRAb in the serum of Graves′ patients, and improve the prognosis of Graves′ disease.

3.
Article in Chinese | WPRIM | ID: wpr-908707

ABSTRACT

Objective:To investigate the value of Doppler ultrasonography in the determination of uterine artery data in early pregnancy for predicting gestational hypertension.Methods:Seventy patients with gestational hypertension treated in Jiashan First People′s Hospital from March 2018 to January 2020 were selected as the case group, and 70 healthy pregnant women who received routine prenatal examinations during the same period were selected as the control group. The differences of uterine artery data and pregnancy outcomes between the two groups at 11-13 +6 weeks of pregnancy were compared, and the value of each indicator in predicting gestational hypertension were analyzedby receiver operating characteristic (ROC) curve. Results:The uterine artery resistance index (RI), pulsatility index (PI), number of bilateral notches in the case group were higher than those in the control group: 0.54 ± 0.12 vs. 0.43 ± 0.08, 0.97 ± 0.36 vs. 0.69 ± 0.31, 12.86%(9/70) vs. 1.43%(1/70), and the differences were statistically significant ( P<0.05). The neonatal weight in the case group was lower than that in the control group: (2 912.38 ± 528.07) g vs. (3 487.39 ± 416.73) g; the intrauterine growth retardation rate, asphyxia rate and preterm birth rate in the case group were higher than those in the control group: 11.43%(8/70) vs. 1.43%(1/70), 8.57%(6/70) vs. 0, 15.71%(11/70) vs. 4.29%(3/70), the differences were statistically significant ( P<0.05). The analysis of ROC curve showed that the area under the curve(AUC) of PI in predicting gestational hypertension was the highest, and when AUC was 0.798, the sensitivity and specificity of PI in predicting gestational hypertension were 81.00% and 86.00% respectively. Conclusions:The uterine artery blood flow in pregnant women with gestational hypertension has been abnormal in early pregnancy (11-13 +6 weeks). The parameters of Doppler ultrasonography is used to predict gestational hypertension, and PI showes better predictive efficacy.

4.
Article in Chinese | WPRIM | ID: wpr-908400

ABSTRACT

Objective:To investigate the current situation of children′s awareness of disaster prevention and self-rescue ability in Mianyang area, provide reference for the development of scientific training programs on children′s disaster knowledge, and improve children′s survival ability during the disaster occurence.Methods:Through literature review and expert consultation, the questionnaire was designed by ourselves.Multi-stage sampling method was used to investigate 2 162 cases from county (city) high school, junior high school and primary school in Mianyang area.Results:8.3% of children had weak disaster awareness.Children had a good understanding of disaster related knowledge, with the highest alarm call awareness rate of 91.8%, fire, flood, earthquake escape and earthquake warning score of 86.5%, 84.2%, 84.2%, 83.0%.Cardiopulmonary resuscitation and fire hydrant operation awareness rate was very low (58.8%, 57.4%). Even some children still chose improper escape behavior.The scores of early warning knowledge and first aid skill were correlated with gender and age.In knowledge of disaster precursors, using of fire extinguishers and hydrants, and first aid skills, the scores of boys were significantly higher than those of girls ( P<0.001). Except for fire-related knowledge, gas leakage escape and shelter awareness rate, the older the children were, the higher the scores were ( P<0.05). The scores of early warning, escape knowledge and first aid skills were correlated with the location of home, and the differences were significant ( P>0.001). More than 80.0% of children chose flashlights, food and water, and emergency kits, while 65.0% to 75.0% children thought it was not necessary to prepare a radio, telephone directory, and cash. Conclusion:Children′s awareness of disaster reduction and self-rescue ability are generally good, but there are still insufficient first-aid skills and improper choice of escape behavior.The education of disaster awareness and self-rescue ability should be strengthened in children′s basic education so as to reduce the casualties of children caused by disasters.

5.
Article in Chinese | WPRIM | ID: wpr-908283

ABSTRACT

Objective:To investigate the effects of beginning time of intermittent pneumatic compression (IPC) on hemodynamics and deep vein thrombosis (DVT) of patients with major orthopedic surgery.Methods:A total of 99 patients who underwent major orthopaedic surgery in the Department of Orthopaedics, West China Hospital, Sichuan University from January 2018 to December 2019 were selected as the research objects, which were assigned to ultra-early group, early group and control group, each group contained 33 cases. The IPC were used 3 days before surgery in the ultra-early group, 1 day before surgery in the early group, and after surgery in control group. The incidence of DVT and deep skin pressure injury within 14 days was observed, the blood flow velocity of deep femoral vein and plasma D-dimer in the three groups were also compared.Results:There was no significant difference in the incidence of DVT among the three groups ( P> 0.05), however, the deep tissue injury rate was 18.18% (6/33) in the ultra-early group, significantly higher than 3.03% (1/33) in the early group and 0 in control group, the difference was statistically significant ( χ2 value was 9.531, P<0.05). After 1 day and 3, 5, 7 days of surgery, the blood flow velocity of deep femoral vein in the ultra-early group and the early group were (26.48±2.24), (25.79±2.18), (26.67±3.74), (25.88±2.83) ml/s and (25.76±1.87), (25.39±1.98), (25.45±2.93), (25.48±3.75) ml/s, significantly higher than (23.39±1.75), (23.73±2.61), (23.79±2.30), (22.21±4.42) ml/s in the control group, the difference was statistically significant ( F values were 7.428-22.350, P<0.01). After 3, 5, 7 days of surgery, the levels of plasma D-dimer in the ultra-early group and the early group were (1.11±0.26), (1.03±0.23), (0.98±0.28) mg/L and (1.18±0.32), (1.12±0.24), (1.05±0.31) mg/L, significantly lower than (1.38±0.40), (1.32±0.39), (1.20±0.26) mg/L in the control group, the difference was statistically significant ( F values were 5.809, 8.442, 4.962, P<0.01). Conclusion:Using IPC one day before operation can significantly increase the blood flow velocity of deep femoral vein, reduce the level of plasma D-dimer, and do not increase the incidence of deep skin pressure injury in patients with major orthopedic surgery.

6.
Article in Chinese | WPRIM | ID: wpr-906265

ABSTRACT

Objective:To investigate the neuroprotective effects of Danggui Shaoyaosan (DSS) on APP<sub>swe</sub>/PS1<sub>ΔE9 </sub>transgenic (APP/PS1) mice and its mechanism related to circular RNA (circRNA). Method:Totally twenty 6-month-old APP/PS1 mice were divided into model group and DSS group, and 10 C57BL/6 wild-type mice were set as the normal control group. The normal group and model group received the same volume of normal saline, and DSS group received drug by gavage administration, all for 8 weeks. The differentially expressed circRNA of APP/PS1 mice before and after DSS intervention was analyzed by circRNA sequencing to construct circRNA-miRNA mRNA interaction network. The results of cricRNA sequencing were then verified by Real-time fluorescence quantitative polymerase chain reaction (Real-time PCR). The protein expression levels of phosphoinositide 3-kinase (PI3K), p-PI3K, protein kinase B1 (Akt1), p-Akt1, B lymphocytoma-2 (Bcl-2), and Bcl-2-Associated X protein (Bax) in the hippocampus were detected by immunoblotting (Western blot). The protein expression of Caspase-3 in the hippocampus was detected by immunohistochemistry and the level of apoptosis in the hippocampus was detected by the TUNEL method. Result:Compared with the model group, there were 90 differentially expressed circRNA after intervention with DSS, of which 46 were up-regulated and 44 down-regulated. Compared with the normal group, the expression levels of circRNA1398 and circRNA1399 in the model group decreased, and the expression levels of miR-103-3p, miR-153-3p, miR-143-3p, and miR-143-5p increased. Compared with the model group, the expression levels of circRNA1398 and circRNA1399 in the DSS group were up-regulated, while the expression levels of miR-103-3p, miR-153-3p, miR-143-3p, and miR-143-5p were down-regulated. Compared with the normal group, the expression of p-PI3K, Akt1, p-Akt1, and Bcl-2 in the model group decreased (<italic>P</italic><0.05,<italic> P</italic><0.01), and the expression of Bax and Caspase in the model group increased (<italic>P</italic><0.01). Compared with the model group, the expression of p-PI3K, Akt1, p-Akt1, and Bcl-2 in the hippocampus of the DSS group increased (<italic>P</italic><0.01), and the protein expression of Bax and Caspase decreased (<italic>P</italic><0.01). Compared with the normal group, the apoptosis level in the hippocampus of the model group increased, with an apoptosis rate of (43.76±2.92)%. Compared with the model group, the apoptosis rate of DSS group was (24.64±3.39)%. Conclusion:DSS can activate PI3K/Akt pathway and inhibit apoptosis in hippocampal neurons of APP / PS1 mice, and play a neuroprotective role. The specific mechanism may be related to the regulation of circRNA1398 and circRNA1399 expression and the corresponding miRNA expression.

7.
Article in Chinese | WPRIM | ID: wpr-906044

ABSTRACT

Objective:To investigate the neuroprotective effect of Danggui Shaoyaosan (DSS) in a rat model of amyloid-<italic>β</italic>-peptide<sub>1-42</sub> (A<italic>β</italic><sub>1-42</sub>)-induced Alzheimer's disease (AD) as well as its regulatory effect on NOD-like receptor protein 3 (NLRP3)/cysteinyl aspartate-specific protease-1 (Caspase-1) signaling pathway. Method:The AD animal model was established via intracerebral injection of A<italic>β</italic><sub>1-42</sub> and treated with different concentrations of DSS after the division of rats into the sham operation group, model group, as well as the high-, medium-, and low-dose DSS groups. Morris water maze test was conducted to determine the learning and memory abilities of rats. The morphology and function of neurons were detected by hematoxylin-eosin (HE) staining and Golgi staining, followed by immunofluorescence co-localization of NLRP3 inflammasome activation. The mRNA expression levels of interleukin (IL)-1<italic>β</italic> and IL-18 were measured by Real-time polymerase chain reaction (Real-time PCR), and the protein expression levels of NLRP3, Caspase-1, and IL-1<italic>β </italic>were assayed by Western blot. Result:Compared with the sham operation group, the model group exhibited significantly decreased learning and memory abilities (<italic>P</italic><0.01), impaired neuronal morphology and function, up-regulated IL-1<italic>β</italic> and IL-18 mRNA expression, enhanced NLRP3 inflammasome activation, and elevated NLRP3, Caspase-1, and IL-1<italic>β</italic> protein expression (<italic>P</italic><0.01). Compared with the model group, DSS at both medium and high doses remarkably improved the learning and memory abilities of AD rats (<italic>P</italic><0.05, <italic>P</italic><0.01), restored neuronal morphology and function, down-regulated the mRNA expression levels of inflammatory factors IL-1<italic>β</italic> and IL-18, reduced the activation of NLRP3 inflammasomes, and lowered the protein expression levels of NLRP3, Caspase-1, and IL-1<italic>β</italic> (<italic>P</italic><0.01). Conclusion:DSS inhibits inflammasome activation and neuroinflammatory response possibly by regulating the NLRP3/Caspase-1 signaling pathway, thus exerting the neuroprotective effect.

8.
Article in Chinese | WPRIM | ID: wpr-904723

ABSTRACT

@#Objective    To explore the predictive value of a simple prediction model for patients with acute myocardial infarction. Methods    Clinical data of 280 patients with acute ST-segment elevation myocardial infarction (STEMI) in the Department of Emergence Medicine, West China Hospital of Sichuan University from January 2019 to January 2020 were retrospectively analyzed. The patients were divided into a death group (n=34) and a survival group (n=246). Results    Age, heart rate, body mass index (BMI), global registry of acute coronary events (GRACE), thrombolysis in myocardial infarction trial (TIMI) score, blood urea nitrogen, serum cystatin C and D-dimer in the survival group were less or lower than those in the death group (P<0.05). Left ventricle ejection fraction and the level of albumin, triglyceride, total cholesterol and low density lipoprotein cholesterol were higher and the incidence of Killip class≥Ⅲ was lower in the survival group compared to the death group (P<0.05). Multivariate logistic regression analysis showed that age, BMI, heart rate, diastolic blood pressure, and systolic blood pressure were independent risk factors for all-cause death in STEMI patients. Receiver operating characteristic (ROC) curve analysis showed that the area under the curve of simple prediction model for predicting death was 0.802, and similar to that of GRACE (0.816). The H-L test showed that the simple model had high accuracy in predicting death (χ2=3.77, P=0.877). Pearson correlation analysis showed that the simple prediction model was significantly correlated with the GRACE (r=0.651, P<0.001) and coronary artery stenosis score (r=0.210, P=0.001). Conclusion    The simple prediction model may be used to predict the hospitalization and long-term outcomes of STEMI patients, which is helpful to stratify high risk patients and to guide treatment.

9.
Article in Chinese | WPRIM | ID: wpr-904624

ABSTRACT

Objective To analyze the components of proteins from Echinococcus granulosus cyst fluid using the shotgun method, and to identify the active components with potential regulatory effects for immune dysregulation diseases. Methods The E. granulosus cyst fluid was collected aseptically from the hepatic cysts of patients with cystic echinococcosis, and characterized by liquid chromatography (LC) tandem mass spectrometry (MS/MS) following digestion with trypsin. The protein data were searched using the software MaxQuant version 1.6.1.0 and the cellular components, molecular functions, and biological processes of the identified proteins were analyzed using the Gene Ontology (GO) method. Results The E. granulosus cyst fluid separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) had a relative molecular mass of 25 to 70 kDa. LS-MS/MS analysis identified 37 proteins, including 32 known proteins and 5 unknown proteins. At least 4 proteins were preliminarily found to exhibit potential regulatory effects for immune dysregulation diseases, including antigen B, glutathione-S-transferase (GST), thioredoxin peroxidase (TPX) and malate dehydrogenase (MDH). GO enrichment analysis showed that the identified proteins had 149 molecular functions and were involved in 341 biological processes. Conclusions E. granulosus cyst fluid has a variety of protein components, and four known proteins are preliminarily identified to be associated with immune dysregulation diseases.

10.
Article in Chinese | WPRIM | ID: wpr-904494

ABSTRACT

Objective:To explore the differences between shanghai water quality standards for drinking water (DB31/T 1091-2018) and domestic and foreign water quality standards, and to compare the improvement of water quality before and after the implementation of DB31/T 1091-2018. Methods:To compare shanghai water quality standard for drinking water with domestic and foreign standards in terms of index number, indicators of disinfectants and their by-products, increased indicators, and revised indicators, and the key water quality indicators before and after the standard implementation in Pudong New Area of Shanghai were investigated. Results:The number of new standard increased from one hundred and six to one hundred and eleven. Among them, the number of conventional indicators increased from forty-two to forty-nine, while the number of unconventional indicators decreased from sixty-four to sixty-two,seven new conventional indicators were added, and the limit value of seventeen conventional indicators was revised and so on. By analyzing water quality monitoring data of New standard before implementation (2017) and after implementation (2019) at water plants of Pudong New Area of Shanghai , it is found that the total number of bacteria (Z=-2.772,P<0.01), and carbon tetrachloride (Z=-5.570,P<0.01),trichloromethane (Z=-5.685,P<0.01), turbidity (Z=-4.168,P<0.01), total dissolved solids (Z=-7.061,P<0.01), and total hardness (Z=-2.338,P<0.05), oxygen consumption (Z=-2.580,P<0.05), anion synthetic detergent (Z=-2.162,P<0.05), total chlorine (Z=-2.826,P<0.01), there were significant statistical differences between the two groups, while there were no statistical differences between the other indicators. Conclusion:The new standard refers to the domestic and foreign drinking water standards, tends to the high and strict requirements, and pushes the improvement water quality of the centralized water supply units, which need to carry out in-depth treatment process transformation, in order to further optimize the water quality.

11.
Protein & Cell ; (12): 877-888, 2021.
Article in English | WPRIM | ID: wpr-922482

ABSTRACT

A new coronavirus (SARS-CoV-2) has been identified as the etiologic agent for the COVID-19 outbreak. Currently, effective treatment options remain very limited for this disease; therefore, there is an urgent need to identify new anti-COVID-19 agents. In this study, we screened over 6,000 compounds that included approved drugs, drug candidates in clinical trials, and pharmacologically active compounds to identify leads that target the SARS-CoV-2 papain-like protease (PLpro). Together with main protease (M


Subject(s)
Antiviral Agents/therapeutic use , Binding Sites , COVID-19/virology , Coronavirus Papain-Like Proteases/metabolism , Crystallography, X-Ray , Drug Evaluation, Preclinical , Drug Repositioning , High-Throughput Screening Assays/methods , Humans , Imidazoles/therapeutic use , Inhibitory Concentration 50 , Molecular Dynamics Simulation , Mutagenesis, Site-Directed , Naphthoquinones/therapeutic use , Protease Inhibitors/therapeutic use , Protein Structure, Tertiary , Recombinant Proteins/isolation & purification , SARS-CoV-2/isolation & purification
12.
Journal of Experimental Hematology ; (6): 1462-1470, 2021.
Article in Chinese | WPRIM | ID: wpr-922280

ABSTRACT

OBJECTIVE@#To screen the serum differentially expressed proteins of APL in children.@*METHODS@#Serum protein expression profiles from 20 cases of normal healthy controls, and 20 cases of APL patients were detected by iTRAQ (isobaric tag for relative and absolute quantification)labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry(2DLC-MS/MS), and analyzed by bioinformatics software. S100A8, LRG1 and SPARC were validated by ELISA. ROC was built by SPSS 20.0 software.@*RESULTS@#Analysis identified 83 differentially expressed proteins in APL serum compared with control according to our defined criteria, of which 33 proteins were up-regulated and 50 proteins were down-regulated (P<0.05).IPA analysis revealed that these differentially expressed proteins were related to the function of Cellular Movement, Immune Cell Trafficking, Hematological System Development and Function, Cell-To-Cell Signaling and Interaction, Tissue Development, and involved in a variety of signalling Pathways, the most representative pathways including LXR/RXR Activation and Acute Phase Response Signaling. S100A8 and LRG1 were found to be elevated and SPARC was markedly down-regulated in serum of childhood APL when compared to the normal controls as examined by ELISA (P<0.05), which was consistent with the iTRAQ result. The overall predictive accuracy of each protein was reflected by the area under the ROC curve(AUC), S100A8,LRG1 and SPARC with ROC areas of 0.841,1.000 and 0.944 respectively.@*CONCLUSION@#S100A8,LRG1 and SPARC may be serve as serum candidate biomarkers for pediatric APL.


Subject(s)
Blood Proteins , Child , Chromatography, Liquid , Humans , Leukemia, Promyelocytic, Acute , Proteomics , Tandem Mass Spectrometry
13.
Chinese Acupuncture & Moxibustion ; (12): 1216-1220, 2021.
Article in Chinese | WPRIM | ID: wpr-921035

ABSTRACT

OBJECTIVE@#To compare the effect of moxibustion combined with basic treatment and simple basic treatment on the clinical symptoms, renal function and hypercoagulable state in patients with idiopathic membranous nephropathy (IMN) of low to medium risk with spleen-kidney deficiency and blood stasis.@*METHODS@#A total of 60 patients with IMN of low to medium risk with spleen-kidney deficiency and blood stasis were randomized into an observation group (30 cases, 2 cases dropped off) and a control group (30 cases, 1 case dropped off). In the control group, the conventional basic treatment of anti-hypertension, regulating blood lipid and anti-coagulation was adopted. On the basis of the control group, moxibustion was applied at Shenshu (BL 23), Pishu (BL 20), Guanyuan (CV 4), Zusanli (ST 36) and Sanyinjiao (SP 6) in the observation group, once a day, 5 days a week continuously with 2 day interval. The treatment of 6 months was required in the both groups. Before treatment and 3 and 6 months into treatment, the total TCM syndrome score, the renal function indexes (24-hour urinary protein quantity [UTP], albumin [ALB], urea nitrogen [BUN] and creatinine [Scr]), the blood coagulation indexes (fibrinogen [FIB], D-Dimer [D-D], p-selection and von Willebrand factor [vWF]), total cholesterol (TC) and triacylglycerol (TG) levels were observed, and the therapeutic efficacy was evaluated on 3 and 6 months into treatment in the two groups.@*RESULTS@#The effective rates of 3 and 6 months into treatment were 78.6% (22/28) and 89.3% (25/28) in the observation group, which were higher than 62.1% (18/29) and 75.9% (22/29) in the control group respectively (@*CONCLUSION@#Moxibustion combined with basic treatment can effectively improve the clinical symptoms, renal function and renal microcirculation in patients with idiopathic membranous nephropathy of low to medium risk with spleen-kidney deficiency and blood stasis, the therapeutic effect is superior to the simple basic treatment.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Glomerulonephritis, Membranous , Humans , Kidney/physiology , Moxibustion , Spleen
14.
Article in Chinese | WPRIM | ID: wpr-910794

ABSTRACT

Objective:To investigate the feasibility of maximum standardized uptake value (SUV max) of extraocular muscle during 99Tc m-diethylene triamine pentaacetic acid (DTPA) orbital SPECT/CT imaging for the clinical diagnosis and evaluation of thyroid associated ophthalmopathy (TAO). Methods:From July 2019 to April 2020, 38 patients (13 males, 25 females, age: 15-77 (48.8±13.7) years; 76 eyes) diagnosed with TAO and 11 healthy volunteers (2 males, 9 females, age: 20-87 (53.5±20.2) years; 22 eyes) were enrolled retrospectively from the Second Hospital of Dalian Medical University. According to the clinical activity score (CAS), 76 eyes were divided into active group (46 eyes) and inactive group (30 eyes). All subjects underwent 99Tc m-DTPA orbital SPECT/CT imaging, then SUV max and uptake ratio (UR) of extraocular muscles were measured and calculated by 3 nuclear medicine physicians. The corrected SUV max was obtained after correction. Kruskal-Wallis rank sum test and Bonferroni test were used to compare the differences of corrected SUV max and UR among the three groups. Spearman rank correlation analysis was used to analyze the correlation between corrected SUV max /UR and CAS. The receiver operating characteristic (ROC) curve analysis was used to evaluate the diagnostic efficacy of corrected SUV max and UR. Intra-class correlation coefficient (ICC) was used for reliability analysis to evaluate the consistency of corrected SUV max and UR among the 3 measurers. Results:The corrected SUV max of the extraocular muscles in the active group, inactive group and control group were 15.96(10.99, 19.63), 7.98(6.80, 9.28), 7.59(6.59, 8.20) respectively ( H=42.196, P<0.001), and UR were 2.17(1.65, 2.79), 1.22(1.03, 1.39), 1.12(0.99, 1.36) respectively ( H=40.642, P<0.001). The corrected SUV max and UR of the extraocular muscles in the active group were significantly higher than those in the inactive group ( H values: -4.971, -5.053, both P<0.001) and in the control group ( H values: -5.681, -5.436, both P<0.001), while there was no significant difference between the inactive group and the control group ( H values: -1.190, -0.796, both P>0.05). Both corrected SUV max and UR were positively correlated with CAS ( rs values: 0.653, 0.615, both P<0.001). The area under the ROC curve of corrected SUV max was 0.851, and the threshold value of corrected SUV max for distinguishing active and inactive periods was 10.125, with the sensitivity of 80.4%(37/46) and the specificity of 86.7%(26/30). The area under the ROC curve of UR was 0.845, and the threshold value for differentiating active and inactive periods was 1.565, with the sensitivity of 80.4%(37/46) and the specificity of 86.7%(26/30). The ICC of corrected SUV max and UR were 0.966(95% CI: 0.953-0.976, P<0.001) and 0.618(95% CI: 0.436-0.744, P<0.001) respectively. Conclusion:Compared with UR, SUV max may be a more promising index for estimating disease activity of orbits in patients with TAO.

15.
International Eye Science ; (12): 1746-1750, 2021.
Article in Chinese | WPRIM | ID: wpr-886717

ABSTRACT

@#In recent years, with the development of social economy, the progress of science and technology and the change of life style, the prevalence of myopia has increased year by year and has a younger trend, and with the outbreak of COVID-19, children and adolescents attending short-distance online classes for a long time has accelerated the occurrence and development of myopia. Visual impairment caused by myopia and serious complications caused by high myopia have seriously affected people's quality of life, study and work. Therefore, the World Health Organization, the International Association for the Prevention of Blindness and China's health departments have put the prevention and control of myopia among children and adolescents on the agenda. However, the etiology is complex, and now it is considered to be related to genetic, environmental and other factors, which is briefly discussed in this review.

16.
Cancer Research and Clinic ; (6): 204-208, 2021.
Article in Chinese | WPRIM | ID: wpr-886035

ABSTRACT

Objective:To explore the effect of goal-directed fluid therapy (GDFT) on the gastrointestinal function of patients after laparoscopic radical resection of cervical cancer.Methods:A total of 60 patients who were scheduled for laparoscopic radical resection of cervical cancer in Shanxi Provincial People's Hospital from October 2016 to September 2018 were selected. They were randomly divided into observation group and control group by random number table method, with 30 cases in each group. Patients in the observation group received GDFT, they were connected to the Flotrac/Vigile monitoring system, and the fluid supplementation was guided according to the changes in mean arterial pressure (MAP), stroke volume variability (SVV) and cardiac index, the goal was to maintain MAP≥60 mmHg (1 mmHg = 0.133 kPa), SVV≤13%, and cardiac index 2.5-4.0 L·min -1·m -2. The conventional fluid therapy was applied in the control group, and the liquid's input speed was adjusted according to the changes of MAP and central venous pressure (CVP) which were respectively maintained at 60-110 mmHg and 8-12 cmH 2O (1 cmH 2O = 0.098 kPa). The crystal/colloid input, bleeding volume and urine output were recorded. The first bowel sounds recovery time, exhaust time, postoperative hospitalization time, and the incidence of nausea and vomiting after surgery were recorded. Arterial blood and central venous blood were drawn before anesthesia induction and 12, 24 and 36 hours after operation to determine the concentrations of arterial blood lactate and central venous oxygen saturation (ScvO 2) as well as intestinal type fatty acid binding protein (IFABP). Results:Compared with the control group, the urine output was increased ( t = -7.738, P < 0.01), the crystal input was reduced ( t = -13.439, P < 0.01), the colloid input was increased ( t = -8.360, P < 0.01), the recovery time of first bowel sounds after surgery was shortened ( t = 6.694, P < 0.01), the exhaust time was shortened ( t = -10.326, P < 0.01), and the time of postoperative hospitalization was shortened ( t = -7.377, P < 0.01). The incidence of nausea and vomiting in the observation group were 10.0% (3/30) and 6.7% (2/30), which were lower than 33.3% (10/30) and 26.7% (8/30) in the control group ( χ2 = 4.812, P = 0.028; χ2 = 4.320, P = 0.038). Compared with the control group, the concentration of IFABP in the observation group was reduced at 12 h ( t = 2.983, P = 0.004), 24 h ( t = 6.452, P < 0.01), and 36 h ( t = -3.880, P < 0.01) after surgery; the concentration of lactate in the observation group was reduced at 12 h ( t = -7.377, P < 0.01), 24 h ( t = -6.036, P < 0.01), and 36 h ( t = -8.933, P < 0.01) after surgery; the value of ScvO 2 in the observation group was increased at 12 h ( t = 2.710, P = 0.009) and 24 h ( t = 2.387, P = 0.020) after surgery. Conclusion:GDFT can maintain the balance between oxygen delivery and oxygen consumption in the gastrointestinal mucosa cells, which can promote the recovery of gastrointestinal function of patients undergoing laparoscopic radical resection of cervical cancer.

17.
Article in Chinese | WPRIM | ID: wpr-883205

ABSTRACT

Objective:To summarize and analyze the clinical characteristics of different treatment in children with esophageal foreign bodies.Methods:This study collected 246 children with esophageal foreign bodies in our hospital from January 2016 to January 2020, which was divided into endoscopic group and operative group.The general and clinical data of children treated with different treatment were collected and statistical analyzed.Results:There were 222 children in endoscopic group and 24 children in operative group, respectively.The rate of surgery was 9.75%.There were no significant differences in gender and location of esophageal foreign bodies.However, the average age of operative group was(2.92±2.67) years, which significantly younger than that in endoscopic group(4.12±3.37)years( P=0.049). The residence time in operative group(median 29.10 h)was remarkable longer than that in operative group(median 11.80 h)( P<0.001). The proportion of sharpness(50.00%) and corrosive(45.83%) foreign bodies in operative group were more than those in endoscopic group[16.22% and 8.11%( P<0.001)]. Moreover, the occurrence rate of major complication in operative group was 83.33%, which was dramatically higher than that in endoscopic group(0.90%)( P<0.001). Conclusion:The younger and longer residence time of esophageal foreign bodies in children contribute to the rate of operative treatment.Additionally, the sharpness and corrosive foreign bodies increase the risk of surgery and serious complications.

18.
Article in Chinese | WPRIM | ID: wpr-881254

ABSTRACT

@#The patient, male, 1 year, was admitted to our hospital with cardiac murmur. Cardiac ultrasonography showed "complete atrioventricular septal defect (C-AVSD), secondary orifice atrial septal defect (ASD), patent ductus arteriosus (PDA), left superior vena cava, and pulmonary hypertension". The patient got follow-up at the age of 3, 6, 9 months and 1 year, with no feeding difficulties, no obvious underdevelopment and no history of repeated respiratory infections. Cardiac ultrasonography showed that the ventricular septal defect (VSD) healed spontaneously at 9 months of age. At 1 year of age, he was admitted to the hospital with "partial atrioventricular septal defect (P-AVSD)" and accepted surgery. Intraoperative exploration showed that the primary orifice ASD was 12 mm, the atrioventricular valve was divided into two groups, and the left atrioventricular valve had three leaflets: anterior, posterior, and lateral one. A cleft was between the anterior and posterior leaflets. The annulus was not enlarged with diameter of 13 mm. The right atrioventricular valve developed well, with fibrous hyperplasia and adhesion under the septal valve. No VSD was seen. The cleft was sutured intermittently. Autologous pericardial patch was used to repair the primary orifice ASD, and the coronary sinus was separated into the right atrium. Self-healing of VSD patients with C-AVSD is very rare, suggesting that patients with C-AVSD with normal range of development, and without obvious clinical symptoms and secondary damage, should be followed up and accept elective surgery in clinical practice.

19.
Article in Chinese | WPRIM | ID: wpr-879133

ABSTRACT

Nine secondary metabolites(S)-5-hydroxy-4-methylchroman-2-one(1), 4-methoxynaphthalene-1,5-diol(2), 8-methoxynaphthalene-1,7-diol(3), 1,8-dimethoxynaphthalene(4),(2R,4S)-2,3-dihydro-2-methyl-benzopyran-4,5-diol(5),(2R,4R)-3,4-dihydro-4-methoxy-2-methyl-2H-1-benzopyran-5-ol(6), 7-O-α-D-ribosyl-2,3-dihydro-5-hydroxy-2-methyl-chromen-4-one(7),(R)-3-methoxyl-1-(2,6-dihydroxyphenyl)-butan-1-one(8) and helicascolide A(9) were isolated from endophytic fungus Cladosporium sp. JJM22 by using column chromatographies of silica gel and ODS, and semi-preparative HPLC. Their structures were analyzed on the basis of spectroscopic and chemical data, especially NMR and MS. All isolated compounds were evaluated for their anti-inflammatory activities by examining the inhibitory activities on nitric oxide(NO) production induced by lipopolysaccharide in mouse macrophage RAW264.7 cells in vitro. Compounds 2-4 showed inhibitory activities.


Subject(s)
Animals , Benzopyrans , Cladosporium , Fungi , Mice , Molecular Structure , Rhizophoraceae
20.
Article in Chinese | WPRIM | ID: wpr-879076

ABSTRACT

This study aims to investigate the preventive effect of Dendrobium officinale in LPS-induced intestinal mucosal damage. Forty SPF-grade C57 BL/6 J male mice were randomly divided into normal group(NC), model group(LPS), and two superfine powder groups of Dendrobium officinale(DOF)(DOF-L, 0.30 g·kg~(-1)and DOF-H, 0.60 g·kg~(-1), respectively), with 10 mice in each group. DOF superfine powder suspension was given via oral administration to mice for 7 days, while the mice in NC and LPS groups received the same volume of saline for 7 days. On the eighth day, the mice in LPS group and DOF treatment groups were injected with LPS(5 mg·kg~(-1)) by intraperitoneal injection to establish the intestinal mucosal injury model, while the mice in NC group were injected with the same volume of sterile saline in the same manner. Six hours after injection with LPS or saline, plasma and the intestinal tissue were collected. The diamine oxidase(DAO) and D-lactate levels in plasma were detected with a biochemical method. The levels of proinflammatory factors interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in plasma were detected by ELISA. The histomorphology and ultrastructure of mouse ileum tissues were observed by hematoxylin-eosin(HE) staining in optical microscope and transmission electron microscope(TEM). The expression and distribution of tight junction(TJ) proteins claudin-1, occludin and F4/80 were detected by immunohistochemistry while the protein expression levels of Toll-like receptor 4(TLR-4) and nuclear factor kappa B p65(NF-κB p65) in jejunum were detected by Western blot. The experimental results showed that continuous intragastric administration of D. officinale superfine powder for 7 days obviously alleviated the damage and ultrastructural changes of intestinal mucosa induced by LPS; significantly decreased DAO and D-lactate levels in plasma in model group(P<0.05); up-regulated the protein expression of claudin-1 and occludin in ileum tissues; down-regulated the protein expression of TLR-4 and NF-κB p65 in jejunum tissues(P<0.01); significantly decreased TNF-α and IL-6 levels in plasma(P<0.05); and decreased the infiltration of F4/80~+ macrophage cells. Our results suggested that D. officinale had significant protective effects on LPS-induced intestinal mucosal damage and reduced intestinal permeability. The mechanism might be related to its effects of inhibiting inflammation via TLR-4/NF-κB p65, and up-regulating the expression of tight junction proteins.


Subject(s)
Animals , Dendrobium , Intestinal Mucosa , Lipopolysaccharides , Male , Mice , NF-kappa B , Powders , Tumor Necrosis Factor-alpha/genetics
SELECTION OF CITATIONS
SEARCH DETAIL