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Article in Chinese | WPRIM | ID: wpr-888091


The formulation of expert consensus on clinical application of Chinese patent medicines, in means of exploring the effective combination of experience and evidence to form a research method in line with the characteristics of Chinese patent medicines, is an important transitional stage for clinical researches on Chinese patent medicines. Pre-searching is a new step in the formulation of expert consensus on clinical application of Chinese patent medicines. Before steps of interview and investigation on clinical application, pre-searching is conducted to collect publications and literature on certain variety and similar Chinese patent medicines; the publications on related medical classics and formulas of this variety; the recommendation condition of this variety in clinical practice guidelines and expert consensus; and the medication regimens recommended in disease-specific guidelines. Pre-searching is designed to know about the advantages of certain variety of Chinese patent medicine as well as its potential problems recorded in the literature, which is helpful to find out the clinical positioning of Chinese patent medicines, develop reasonable clinical questions and provide ideas for formal literature searching. However, it is not the direct basis for developing clinical questions. Moreover, interviews and investigations are still needed to further clarify the clinical positioning of Chinese patent medicines and develop reasonable questions. This paper took expert consensus on clinical application of Yanshen Jianwei Capsules as an example to introduce the pre-searching process and methods used during formulation of expert consensus on clinical application of Chinese patent medicines, and to further discuss the role of pre-searching to facilitate the formulation of clinical questions on selection of participants, interventions, controls and outcomes.

Capsules , China , Consensus , Drugs, Chinese Herbal , Humans , Medicine, Chinese Traditional , Nonprescription Drugs
Acta Pharmaceutica Sinica ; (12): 1699-1706, 2020.
Article in Chinese | WPRIM | ID: wpr-823314


The near-infrared-IIb (NIR-IIb, 1 500-1 700 nm) window fluorescence with long emission wavelength has reduced light scattering and tissue auto-fluorescent background, achieving deep tissue imaging with high spatial resolution. Herein, we prepared an NIR-IIb fluorescent quantum dots (QDs) composed of lead sulfide (PbS). The fluorescence spectrum of PbS QDs were adjusted by controlling the size of the PbS core. Cadmium sulfide (CdS) shell was synthesized by the cation exchange method to form the core/shelled lead sulfide/cadmium sulfide quantum dots (CSQDs). The surface of CSQDs was modified with polyethylene glycol (PEG) to increase their stability in aqueous solution. The resulting PEG-modified CSQDs (PEG-CSQDs) had the emission peak at ~1 550 nm with quantum yield of 7.2%. The animal procedures were approved by the Institutional Animal Care and Use Committee (IACUC) of Fudan University School of Pharmacy. At 2 h postinjection, PEG-CSQDs clearly delineated the tumor region of mice bearing orthotopic CT26-Luc colon cancer model in the NIR-IIb fluorescence imaging. The fluorescent intensity ratio of primary tumor and adjacent normal tissue was 42.3, and that of metastatic tumor and adjacent normal tissue was 22.3, which allowed to detect the primary tumor of 3.4 mm×2.5 mm in dimension and the metastatic tumor of 1.2 mm×0.9 mm in dimension, and accurately guided the excision of tumors. The PEG-CSQDs prepared in this study provided a new approach for the early diagnosis and guidance of surgical resection of colon cancer.

Chinese Pharmacological Bulletin ; (12): 1279-1283, 2019.
Article in Chinese | WPRIM | ID: wpr-857155


To study the effect of in vitro fertilization-embryo transfer (IVF-ET) on the contractile function of mesenteric vessels in offspring mouse model and its regulatory mechanism. Methods Offspring mouse model of IVF-ET (20 weeks after birth) was built to compare with natural born descendant in vascular regulation. The bodyweight and BMI of mice were measured. Serum levels of cardiovascular related cytokines were tested by ELISA and colorimetric method. Samples of mesenteric vascular were from IVF-ET mice and normal controls. Given the vital function in regulating vascular contraction which Ca2+signaling pathway exhibited , four representative genes (Calm2, Itprl, RyR2, RyR3) were selectedfor study. And the mRNA expression levels of Calm2, Itpr2, RyR2, RyR3 were tested by qPCR. The protein expression levels of Calm2 and Itprl were tested by Western blot. Results Mice born under IVF-ET showed increased bodyweight and abnormal BMI after 20th week, and the serum levels of NO and Ang II were significantly higher than those of control (P < 0. 0 5) . The expression levels of Calm2, Itprl were up-regulated both in molecular and protein levels (P <0. 0 1) , RyR3 was up-regulated in molecular level (P < 0. 01) , while RyR2 was down-regulated in molecular level (P < 0. 0 1) . Conclusions The changes of serological markers and regulatory gene expression level related to vasoconstriction function may be closely related to the increased risk of cardiovascular diseases in IVF-ET offspring.

Article in Chinese | WPRIM | ID: wpr-273799


<p><b>OBJECTIVE</b>To investigate performance of a biotinylated imaging probe 3a for targeted imaging of breast cancer cells.</p><p><b>METHODS</b>Ultraviolet absorption spectrum and fluorescence spectrum were employed to analyze the spectral characteristics of 3a. The fluorescence spectrums of 3a treated with different concentrations of glutathione (GSH) were obtained to determine the sensibility of 3a to GSH. Flow cytometry was used to determine the cellular uptake of 3a by MCF-7 cells, MDA-MB-231 cells and Hs 578Bst cells in the presence or absence of biotin, and the imaging performance of 3a in the 3 cell lines was assessed under an inverted fluorescent microscope. The toxicity of 3a to the cells was evaluated using MTT method.</p><p><b>RESULTS</b>3a showed the strongest absorption peak at 510 nm, and its fluorescence emission signal was the strongest at 544 nm. As the concentration of GSH increased (0-6 mmol/L), 3a exhibited an increasing fluorescence signal at 544 nm. The cellular uptake of 3a was markedly higher in MDA-MB-231 cells and MCF-7 cells than in Hs 578Bst cells. The imaging studies showed that 3a had a good breast cancer cell-targeting property and produced clear images under fluorescent microscope. MTT assay demonstrated no obvious toxicity of 3a in Hs 578Bst cells even at the concentration of 20 µmol/L, but MCF-7 cells and MDA-MB-231 cells exposed to 2-20 µmol/L 3a showed a lowered cell viability.</p><p><b>CONCLUSION</b>3a is capable of targeted imaging of breast cancer cells mediated by biotin. 3a at the concentration of 2-20 µmol/L has minimal cytotoxicity to normal breast cells but can lower the viability of breast cancer cells.</p>