ABSTRACT
Objective To explore the effectiveness of virtual autopsy-based postmortem computed tomography(PMCT)liver three dimensional slicer(3D slicer)artificial intelligence(AI)volume reconstruction to assist forensic practice.Methods Twenty cases of the deceased who underwent both virtual autopsy and traditional autopsy in our center were selected and subjected to liver volume segmentation by 3D slicer method,Tada's formula method and literature method,and the data obtained from the traditional autopsy were compared and analyzed to obtain the accuracy rate.Results The 3D slicer method yielded higher consistency(95%confidence interval),lower volumetric variability(standard deviation),and a smaller region(variance)of uncertainty than the Tada formula method and the methods mentioned in the literature.Conclusion 3D slicer AI reconstruction based on virtual autopsy can visualize virtual anatomy,help increase the diagnostic accuracy of traditional autopsy,assist in pathological diagnosis,and provide new directions and tools for the development of imaging histology of virtual autopsy.
ABSTRACT
Objective To study the effect of microwave irradiation on a marine mutant strain B1 which had high antifungal activity,and obtain the optimum dosage of microwave irradiation.Methods The marine strain B1(Bacillus sp.) was radiated by 2450 MHz microwave for various times.The mutation rate p,mutation mean ? and the mutation variance ?~(2) were estimated by statistical methods.From these mutants,a high-activity strain B1-413 was obtained,and its genetic attribute was tested.Results Microwave irradiation was highly effective at 60 s.The MIC of mutant strain B1-413 was reduced from 125?l?mL~(-1)to 32?l? mL~(-1) compared with B1,the genetic attribute of which was stable through four generation cultivation.Conclusion The B1 was sensitive to microwave irradiation,and the higher anti-fungi active mutants can be easy obtained after the treatment of microwave irradiation.