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1.
Article in English | WPRIM | ID: wpr-919299

ABSTRACT

Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (PM=3.4%), overall mean abundance (MA=0.36 mites/host) and overall mean intensity (MI=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with CSS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.

2.
Cancer Research and Clinic ; (6): 401-407, 2021.
Article in Chinese | WPRIM | ID: wpr-912896

ABSTRACT

Objective:To investigate effects of capecitabine metronomic chemotherapy combined with exemestane on the proliferation of breast cancer MCF-7 cells and PI3-K/AKT signaling pathway.Methods:MCF-7 cells cultured in vitro were divided into the control group (adding DMEM without drugs), 30 μmol/L exemestane group, capecitabine metronomic chemotherapy combined drugs group [30 μmol/L exemestane combined with different concentrations (50, 33, 17 μmol/L) of capecitabine]. CCK-8 assay was used to detect the cell proliferation inhibition rate, the half-maximal inhibitory concentration ( IC50) was calculated, and the changes of cell cycle and apoptosis rate of MCF-7 in different drug groups were assessed by using flow cytometry. The related-protein expression of PI3K-AKT signaling pathway of MCF-7 cells was detected by using Western blot. Results:The IC50 of capecitabine and exemestane on MCF-7 cells for 72 h was 101.2 μmol/L and 60.6 μmol/L, respectively. The proliferation inhibition rate of MCF-7 cells in 30 μmol/L exemestane for 24 h and 48 h combined with 50, 33 and 17 μmol/L capecitabine group was higher than that in 30 μmol/L exemestane group (all P<0.01). The apoptosis rates were (18.1±2.6)%, (34.6±3.0)%, (27.6±1.3)%, (23.1±1.6)%, respectively in 30 μmol/L exemestane group, 30 μmol/L exemestane + 50 μmol/L capecitabine group, 30 μmol/L exemestane + 33 μmol/L capecitabine group, 30 μmol/L exemestane + 17 μmol/L capecitabine group, and the difference was statistically significant ( F = 23.652, P<0.01). Compared with the control group, the proportion of MCF-7 cells in phase G 2 of 30 μmol/L exemestane group was increased [(16.7±2.6)% vs. (10.6±2.2)%], while that in phase G 1 was decreased [(53.3±4.0)% vs. (56.3±3.2)%]. The proportion of MCF-7 cells in phase S of 30 μmol/L exemestane + 50 μmol/L capecitabine group was increased [(39.0±3.6)% vs. (33.1±2.0)%]. MCF-7 cells of 30 μmol/L of exemestane + 33 μmol/L capecitabine group were more blocked in phase S [(51.7±4.1)%], and cells in phase G 2 were nearly disappeared [(1.2±0.5)%]; the cell proportion MCF-7 cells in phase G 2 of 30 μmol/L exemestane plus 17 μmol/L capecitabine group was increased [(26.2±3.1)%]. Western blot analysis showed that low dose capecitabine metronomic chemotherapy promoted exemestane to inhibit the expression of PI3K, motivated AKT serine phosphorylated at protein 473 [the increased expression of p-AKT (473)], promoted S6 protein expression at downstream of signaling pathway and increased its phosphorylation level (the increased expression of p-S6), thereby activating apoptosis signal. Conclusion:Capecitabine metronomic chemotherapy combined with exemestane can synergistically inhibit the proliferation of breast cancer MCF-7 cells and activate apoptosis mechanisms of MCF-7 cells through affecting PI3K-AKT signaling pathway.

3.
Article in Chinese | WPRIM | ID: wpr-912004

ABSTRACT

Objective:To observe the clinical effect of helmet therapy in cranial remodeling of infants with deformational plagiocephaly.Methods:A total of 114 infants with plagiocephaly received helmet therapy at the age of 4 to 12 months over the period from 2016 to 2018 and the results were analyzed retrospectively. Seventy-four were male and forty female. The average ages were (7.49±1.91) and (7.54±2.10) months, respectively. A Spectra scanner was used to quantify each head′s radial symmetry index (RSI), cranial vault asymmetry (CVA) and cranial vault asymmetry index (CVAI) before and after 100 days of treatment, and those data were related with age (4-6 months, 7-9 months and 10-12 months), gender and severity of deformity (levels III-V).Results:The average RSI, CVA and CVAI improved significantly for all of the infants studied. The average RSI, CVA and CVAI improved the most for those 4-6 months old. Those with level-IV deformity showed significantly better improvement than those with level III or V deformity.Conclusions:Helmet therapy is an effective way to improve deformities due to plagiocephaly among infants. The best age for treatment is 4-6 months. Patients with less malformation are more easily corrected.

4.
Article in Chinese | WPRIM | ID: wpr-911925

ABSTRACT

Objective:To analyze the current situation of the mothers and infants at their first visit to the breastfeeding counseling clinic, and provide a reference for promoting breastfeeding in infancy.Methods:Clinical data of 581 mother-infant-dyads visiting the breastfeeding counseling clinic of Nanjing Maternal and Child Health Hospital from January 2019 to December 2019 were collected and analyzed retrospectively, including the general information of the dyads, and the subjective and actual reasons for visits. According to the age of infants coming to the clinics, they are divided into the younger group (60 d and below, n=316), middle-aged group (61-120 d, n=178), and older group (121 d and above, n=87). Differences in exclusive breastfeeding rate and reasons for visits between different groups were analyzed by analysis of variance, Kruskal-Wallis rank sum test, Chi-square test, or Fisher's exact test. Results:The age of the 581 infants at the first visit were mainly at 30-34, 41-45 and 90-94 d, which accounted for 9.3%( n=54), 25.3%( n=147), and 9.0%( n=52), respectively. The total exclusive breastfeeding rate was 71.8%( n=417) at visit, and there were significant differences among the younger, middle-aged and older group [67.7%(214/316), 79.8%(142/178), 70.1%(61/87), χ 2=14.294, P=0.006), and between the younger and middle-aged group in the pairwise comparison. The most common subjective reason for visiting was slow weight gain ( n=249, 42.9%), followed by worrying about insufficient milk ( n=142, 24.4%) and feeding difficulties ( n=132, 22.7%). Whereas the top three practical reasons for visiting included infant's inability to latch on or sucking ( n=165, 28.4%), lack of breastfeeding knowledge ( n=149, 25.6%) and inappropriate maternal feeding skills ( n=140, 24.1%). The younger group faced more problems regarding latching on or sucking [39.6%(125/316] and mother's feeding skills[25.9%(82/316)], while the lack of breastfeeding knowledge mainly occurred in the older group[48.3%(42/87)]. The coincidence rates between the subjective and actual reasons for slow weight gain, worrying of insufficient milk, and feeding difficulties were only 1.2%( n=3), 6.3%( n=11), and 21.2%( n=28). Conclusions:Breastfeeding- related problems exist in infants of different ages below one year old. There is a big knowledge gap between the subjective reasons for medical visits and the actual problems that interfere with breastfeeding. Therefore, individualized breastfeeding guidance for infants of different ages is recommended in children's health clinics.

5.
Article in Chinese | WPRIM | ID: wpr-875944

ABSTRACT

Objective To evaluate the performance of energy discriminating dosimeter and compare the impact of the assessment results. Methods CTLD-J4000 energy discriminating dosimeter was irradiated with different energy photons, and the differences covered by the dosimeters were used. Density filtering materials have different filtering effects on photons, and the ratio curve of the readings to the photon energy was fitted to identify the photon energy. Results The coincidence between the non-filter/light filter of the CTLD-J4000 energy discrimination dosimeter and the manufacturer's reference value was better than that of the non-filter/re-filter. The latter had higher deviation values at 48 keV(12.8%)and 83 keV(15.5%), respectively. Using the average scale factor, the maximum energy response deviation was 23.5%. Conclusion Using a discriminating dosimeter can better identify the ray energy, thereby reducing the energy response error caused by using the average scale factor.

6.
Journal of Experimental Hematology ; (6): 1436-1449, 2021.
Article in Chinese | WPRIM | ID: wpr-922277

ABSTRACT

OBJECTIVE@#LncRNA ITGB2-AS1 has been found to play important roles in the occurrence and development of human solid tumors. However, its role in hematological diseases, especially acute myeloid leukemia (AML), remains unclear. The aim of this study was to identify the expression pattern of ITGB2-AS1 in AML patients and to further explore its clinical significance.@*METHODS@#ITGB2-AS1 expression was analyzed in public datasets (including TCGA and GSE63270) and further validated in a cohort of 109 AML patients by real-time quantitative PCR (RT-qPCR).@*RESULTS@#The level of ITGB2-AS1 was up-regulated among two independent cohorts (TCGA, P<0.05; GSE63270, P<0.05), which was confirmed by the data from 109 AML patients enrolled in this study (P<0.05). Clinically, high ITGB2-AS1 expression was associated with older age (P=0.023) and lower complete remission (CR) rate (P=0.005). Multivariate analysis identified that high ITGB2-AS1 expression was an independent prognostic factor not only for CR rate (P=0.027) but also for overall survival (OS) time (P=0.011), and ITGB2-AS1 was positively correlated with ITGB2 expression in both TCGA (r=0.74, P<0.001) and clinical data detected in this study (r=0.881, P<0.001). High ITGB2 expression was also associated with older age (P=0.02) and lower CR rate (P=0.020). Moreover, high ITGB2 expression predicted worse OS (P=0.028).@*CONCLUSION@#ITGB2-AS1 is overexpressed in AML and predicts poor prognosis in AML patients.


Subject(s)
Aged , Humans , Leukemia, Myeloid, Acute/genetics , Prognosis , RNA, Long Noncoding/genetics
7.
Article in Chinese | WPRIM | ID: wpr-860956

ABSTRACT

Objective: To observe the feasibility of chemical ablation of left ventricular (LV) anterior papillary muscle (APM) using multifunctional intracardiac echocardiography (ICE) catheter. Methods: Fifteen hybrid canines were randomly divided into 0.2 ml, 0.4 ml and 0.8 ml injection groups (each n=5). Then the above doses of ethanol were injected into the base of LV APM under the location and monitoring of ICE images in each group, respectively. Mitral regurgitation area (MRA) and vena contracta (VC) were measured with transthoracic echocardiography before and 5 days after ablation. All animals were sacrificed 5 days after ablation, the general and pathological changes of APM were observed. Results: ICE images clearly displayed the structure of LV APM and the depth of needle insertion and ablation process in real time. No significant difference of MRA nor VC was detected in 0.2 ml nor 0.4 ml group before and 5 days after ablation, but MRA and VC in in 0.8 ml group were both higher than those before ablation (all P<0.05). Pale ablation lesions in the base of APM were observed, the volume of ablation lesions formed by 0.2 ml, 0.4 ml and 0.8 ml absolute ethanol at the base of APM were (0.37±0.07)cm2, (0.69±0.08)cm2 and (0.96±0.19)cm2, respectively. The volume of ablation lesion in higher dose group was larger than that in lower dose group (all P<0.05). Irreversible necrosis of cardiomyocytes were observed in the ablation site under light microscopy. Conclusion: Chemical ablation of APM is effectively and safely achieved with intramyocardial injection of small amount of ethanol, which may provide a new ablation strategy for treatment of ventricular arrhythmia originating from LV APM.

8.
Article in Chinese | WPRIM | ID: wpr-828053

ABSTRACT

In this study, the growth index including plant height, compound leaf area, specific leaf area, leaf water content, number of branches, and leaf biomass per plant and the icariin flavonoids such as epimedin A, epimedin B, epimedin C and icariin of Epimedium pseudowushanense were determined on 30 d and 60 d under light intensity(18.2±2.5) μmol·m~(-2)·s~(-1)(L1) and(90.9 ±2.5) μmol·m~(-2)·s~(-1)(L2), and white light as control, red light, blue light and yellow light were used as three light quality treatments, to study the effect of light quality on the growth and flavonoids accumulation of E. pseudowushanense. The E. pseudowushanense was sui-table for growth under L1 light intensity, the blue light treatment significantly reduced the leaf area, but had little effect on the stem height, the red light treatment and the yellow light treatment had no obvious effect on the stem height and leaf area, but the yellow light treatment significantly increased the germination of new branches, and had a sustained promoting effect, and the biomass was significantly higher than the white light treatment at 60 d. The content of icariin flavonoids in red light, blue light and yellow light treatment was higher than that in white light treatment at 30 d and 60 d under L1 light intensity, while yellow light treatment promoted the synthesis of icariin flavonoids to the largest extent, which was 1.8 and 1.9 times of white light treatment(30 d and 60 d).Under L2 light intensity, the effect of strong light on promoting stem germination became the main factor, while the yellow light treatment showed no significant effect on promoting stem germination, and the red light treatment exhibited a significant effect on reducing leaf area. Icariin flavonoids under red light, blue light and yellow light treatment were all lower than that under white light treatment, that is, the effect of white light treatment on the synthesis of icariin flavonoids is better than red light, blue light and yellow light treatment. When the time of strong light treatment was longer, the degradation range of icariin flavonoids in other light treatment appeared, while red light treatment promotes the synthesis of icariin flavonoids. Therefore, the influence of light quality on E. pseudowushanense is quite different under different light intensity, no matter from growth index or flavonoid content index. The results support that the biomass and icariin flavonoid content can be increased by providing appropriate red and yellow light.


Subject(s)
Drugs, Chinese Herbal , Epimedium , Flavonoids , Plant Leaves
9.
Chinese Journal of Lung Cancer ; (12): 667-672, 2020.
Article in Chinese | WPRIM | ID: wpr-826967

ABSTRACT

BACKGROUND@#Lung cancer is currently the leading malignant tumor in both domestic and foreign morbidity and mortality. Surgical treatment is the main treatment option for lung cancer. The aim of this study is to explore the effects of enhanced recovery after surgery (ERAS) combined with respiratory function exercise combined with single-hole thoracoscopic surgery on lung cancer patients with postoperative pulmonary complications, postoperative pain, time to get out of bed, time to extubation and length of hospital stay.@*METHODS@#A total of 240 patients who underwent endoscopic lung cancer surgery at the Affiliated Hospital of Yangzhou University and the Yancheng First People's Hospital from October 2017 to October 2019 were randomly divided into 4 groups, with 60 patients in each group. Patients in group A underwent single-hole thoracoscopic surgery, and preoperatively performed ERAS concept education and respiratory function training; group B used conventional 3-hole thoracoscopic surgery, and performed ERAS concept education and respiratory function training before operation; group C used conventional 3-hole thoracoscopic operation surgery, routine hospitalization education and nursing guidance, routine respiratory function training, no preoperative ERAS concept education; group D used single-hole thoracoscopic surgery, routine hospitalization education and nursing guidance, routine respiratory function training, no preoperative ERAS concept mission. The number of postoperative pulmonary complications, postoperative pain, time to get out of bed, extubation time, and hospital stay were recorded in the four groups.@*RESULTS@#Compared with the groups B, C, and D, the incidence of pulmonary complications was significantly reduced, and the time to get out of bed, extubation time, and hospital stay were significantly shortened in group A. Compared with groups B, C, the postoperative pain was significantly reduced in group A. Compared with group C, the pulmonary complications were significantly reduced, and the time to get out of bed, extubation time and hospital stay were significantly shortened in group B. The differences were statistically significant (P0.05).@*CONCLUSIONS@#For patients with single-hole thoracoscopic lung cancer surgery, the ERAS concept guidance can effectively reduce the incidence of pulmonary complications and postoperative pain, shorten the time to get out of bed, the time to extubate, and the length of hospital stay.

10.
Journal of Medical Postgraduates ; (12): 623-626, 2020.
Article in Chinese | WPRIM | ID: wpr-821840

ABSTRACT

ObjectiveTo study the relationship between acute myocardial injury(AMI) and coagulation function in patients with COVID-19.MethodsA retrospective study was carried out to record the general and laboratory data of 133 patients diagnosed with COVID-19 who were hospitalized in Wuhan TongJi Guanggu Hospital, Wuhan, Hubei Province from February 10 to February 29, 2020. The laboratory data includes blood routine, liver and renal function, myocardial infarction tests, coagulation function, inflammatory factors, hypersensitive C-reactive protein, procalcitonin etc. The patients were divided into two groups according to cardiac troponin I(TNI)34.2 ug/L. The differences of general conditions and laboratory data between the two groups were compared. Besides, the correlation between coagulation function and coagulation function, and the ROC curve of D-dimer in AMI were conducted .ResultsAmong the 133 patients, 9 (6.77%) had cTnI greater than 34.2 μg/L, and 124 (93.23%) had normal cTnI. There were significant differences between the two groups in age, COPD history, blood routine (neutrophil count, lymphocyte count, platelet count), myoglobin, liver function (direct bilirubin, indirect bilirubin), cytokines (IL-2 receptor, IL-6, IL-8, IL-10, TNF-α), coagulation function (PT, PTA, D-dimer). D-dimer level was positively correlated with TnI, CK-MB and myoglobin levels. The cut off value of D-dimer was 2.35 μg/ml in acute myocardial injury.ConclusionAcute myocardial injury in COVID-19 patients may be related to coagulation dysfunction. Therefore, monitoring of coagulation function dynamically, screening of thrombus and starting anticoagulant and antiplatelet therapy timely help to reduce acute myocardial injury.

11.
Journal of Medical Postgraduates ; (12): 623-626, 2020.
Article in Chinese | WPRIM | ID: wpr-821820

ABSTRACT

ObjectiveTo study the relationship between acute myocardial injury(AMI) and coagulation function in patients with COVID-19.MethodsA retrospective study was carried out to record the general and laboratory data of 133 patients diagnosed with COVID-19 who were hospitalized in Wuhan TongJi Guanggu Hospital, Wuhan, Hubei Province from February 10 to February 29, 2020. The laboratory data includes blood routine, liver and renal function, myocardial infarction tests, coagulation function, inflammatory factors, hypersensitive C-reactive protein, procalcitonin etc. The patients were divided into two groups according to cardiac troponin I(TNI)34.2 ug/L. The differences of general conditions and laboratory data between the two groups were compared. Besides, the correlation between coagulation function and coagulation function, and the ROC curve of D-dimer in AMI were conducted .ResultsAmong the 133 patients, 9 (6.77%) had cTnI greater than 34.2 μg/L, and 124 (93.23%) had normal cTnI. There were significant differences between the two groups in age, COPD history, blood routine (neutrophil count, lymphocyte count, platelet count), myoglobin, liver function (direct bilirubin, indirect bilirubin), cytokines (IL-2 receptor, IL-6, IL-8, IL-10, TNF-α), coagulation function (PT, PTA, D-dimer). D-dimer level was positively correlated with TnI, CK-MB and myoglobin levels. The cut off value of D-dimer was 2.35 μg/ml in acute myocardial injury.ConclusionAcute myocardial injury in COVID-19 patients may be related to coagulation dysfunction. Therefore, monitoring of coagulation function dynamically, screening of thrombus and starting anticoagulant and antiplatelet therapy timely help to reduce acute myocardial injury.

12.
Article in Chinese | WPRIM | ID: wpr-817580

ABSTRACT

[Objective] To investigate the cure and mortality trend in the early outbreak of Coronavirus Disease 2019 (COVID-19) in China, enhance the entire epidemiological knowledge about COVID-19, screen the more effective evaluation indicators, and supply the solid evidence for assessing the prevention and control measures and input-output. [Methods] The daily new confirmed cases, the cumulative cure and death cases in China and Hubei Province and outside Hubei Province, which were reported officially from January 20, 2020 to February 20, 2020, were collected to calculate the fatality rate, the ratio of the numbers of cumulative cure cases to cumulative death cases, et al, and to make the epidemiological description. [Results] Till Feb 20, 2020, the fatality rate of COVID-19 in Hubei Province was 3.42%, which was a little higher than that of the global country (2.96%). The fatality rate of COVID-19 outside Hubei Province in China was 0.71%. The trends on the indexes, including the daily new cure cases, daily new death and the ratio of cumulative cure cases to cumulative death, in Hubei Province and the whole country was highly consistent, while the significant difference existed between Hubei Province and outside Hubei Province in China. The daily ratio of cumulative cure cases to cumulative death in Hubei Province (the global country) exhibited the U trend, which reached to the lowest point on Jan 27 and subsequently grew more rapidly after Feb 5. [Conclusion] The ratio of cumulative cure cases to cumulative death was easier and effective to reflect the outbreak situation than other absolute indexes like the daily new cure cases or death. Since Feb 5, 2020, the daily cure status of COVID-19 was superior to the daily death, which indicated the effectiveness of series of the joint defense and control measures from our government and society. However, plenty of challenges exist when recovering the normal production and life if the epidemic reaches to be sustainably defensed and controlled.

13.
Chinese Medical Journal ; (24): 185-192, 2020.
Article in English | WPRIM | ID: wpr-877954

ABSTRACT

BACKGROUND@#The Shexiang Baoxin Pill (MUSKARDIA) has been used for treating coronary artery disease (CAD) and angina for more than 30 years in China. Nevertheless, methodologically sound trials on the use of MUSKARDIA in CAD patients are scarce. The aim of the study is to determine the effects of MUSKARDIA as an add-on to optimal medical therapy (OMT) in patients with stable CAD.@*METHODS@#A total of 2674 participants with stable CAD from 97 hospitals in China were randomized 1:1 to a MUSKARDIA or placebo group for 24 months. Both groups received OMT according to local tertiary hospital protocols. The primary outcome was the occurrence of a major adverse cardiovascular event (MACE), defined as a composite of cardiovascular death, non-fatal myocardial infarction (MI), or non-fatal stroke. Secondary outcomes included all-cause mortality, non-fatal MI, non-fatal stroke, hospitalization for unstable angina or heart failure, peripheral revascularization, angina stability and angina frequency.@*RESULTS@#In all, 99.7% of the patients were treated with aspirin and 93.0% with statin. After 2 years of treatment, the occurrence of MACEs was reduced by 26.9% in the MUSKARDIA group (MUSKARDIA: 1.9% vs. placebo: 2.6%; odds ratio = 0.80; 95% confidence interval: 0.45-1.07; P  = 0.2869). Angina frequency was significantly reduced in the MUSKARDIA group at 18 months (P = 0.0362). Other secondary endpoints were similar between the two groups. The rates of adverse events were also similar between the two groups (MUSKARDIA: 17.7% vs. placebo: 17.4%, P = 0.8785).@*CONCLUSIONS@#As an add-on to OMT, MUSKARDIA is safe and significantly reduces angina frequency in patients with stable CAD. Moreover, the use of MUSKARDIA is associated with a trend toward reduced MACEs in patients with stable CAD. The results suggest that MUSKARDIA can be used to manage patients with CAD.@*TRIAL REGISTRATION@#chictr.org.cn, No. ChiCTR-TRC-12003513.


Subject(s)
Angina Pectoris , China , Coronary Artery Disease/drug therapy , Double-Blind Method , Drugs, Chinese Herbal/adverse effects , Humans
14.
Article in Chinese | WPRIM | ID: wpr-876385

ABSTRACT

Objective To investigate the cure and mortality trend in the early outbreak of COVID-19 in China, to increase its entire epidemiological understanding, providing evidence for assessing the prevention and control measures against it and input-output in this regard. Methods Data were collected concerning daily new confirmed cases, the cumulative cure and death cases in China and Hubei Province and outside Hubei Province, which were reported officially from January 20th, 2020 to February 20th, 2020.Calculation was done of the fatality rate, the ratio of the numbers of cumulative cure cases to cumulative death cases, etc, and then the epidemiological description was made. Results As of Feb 20, 2020, the fatality rate of COVID-19 in Hubei Province was 3.42%, which was a little higher than the national figure of 2.96%.The fatality rate of COVID-19 outside Hubei Province in China was 0.71%.The trends on the indexes, including the daily new cure cases, daily new death and the ratio of cumulative cure cases to cumulative death, in Hubei Province and the whole country were found to be highly consistent, while the significant difference existed between Hubei Province and outside Hubei Province in China.The daily ratio of cumulative cure cases to cumulative death in Hubei Province (the whole country) exhibited the U-curve trend, which reached the lowest point on Jan 27 and subsequently rose more rapidly after Feb 5. Conclusion The ratio of cumulative cure cases to cumulative death is easier and effective to reflect the outbreak situation than other absolute indexes like the daily new cure cases or death.Since Feb 5, 2020, the daily cure status of COVID-19 is significantly better than the daily death, which indicates the effectiveness of series of the joint defense and control measures.However, plenty of challenges exist in recovering normal production and life when the epidemic comes to be sustainably prevented and controlled.

15.
China Pharmacy ; (12): 2263-2268, 2020.
Article in Chinese | WPRIM | ID: wpr-825659

ABSTRACT

OBJECTIVE:To systematically evaluate the efficacy and saf ety of sacubitril-valsartan in the treatment of heart failure without reduced ejection fraction (non-HFrEF)patients,and to provide evidence-based reference for its clinical treatment. METHODS:Retrieved from Cochrane Library ,PubMed,Embase,CNKI,VIP and Wanfang data ,during the inception to Feb. 29th,2020,randomized controlled trials (RCTs)about sacubitril-valsartan (trial group )versus routine medicine as renin- angio- tensin converting enzyme inhibitors/angiotensin Ⅱ receptor antagonists (control group ) in the treatment of non-HFrEF were collected. After literature screening and data extraction ,the quality of included literatures were evaluated with Cochrane bias risk evaluation tool 5.3.0. Meta-analysis was conducted with Stata 14.0 software,and the publication bias analysis and sensitivity analysis were performed. RESULTS :Totally 6 RCTs were included ,involving 5 502 patients. Results of Meta-analysis showed that the HF re-hospitalization rate [RR =0.84,95%CI(0.77,0.91),P<0.001] and the serum creatinine elevation rate [RR =0.78,95% CI(0.67,0.91),P=0.001] in trial group were significantly lower than control group. NYHA classification improvement rate [RR = 1.25,95%CI(1.10,1.43),P=0.001] and the hypotension rate [RR =1.43,95%CI(1.24,1.65),P<0.001] were significantly higher than control group. There was no statistical significance in the cardiovascular mortality [RR =0.94,95%CI(0.79,1.12), P=0.481],all-cause mortality [RR =0.95,95%CI(0.83,1.08),P=0.417],the levels of NT-proBNP [WMD =-301.16,95%CI (-602.77,0.44),P=0.050] and LVEF [WMD =1.49,95%CI(-1.33,4.32),P=0.300] after treatment ,and the hyperkalaemia rate [RR =0.88,95%CI(0.77,1.01),P=0.070] between 2 groups. The results of publication bias analysis and sensitivity analysis showed there was a high possibility of publication bias ,and the results of several indexes were not stable. CONCLUSIONS : Sacubitril-valsartan may effectively reduce HF re-hospitalization rate and the risk of elevated serum creatinine in non-HFrEFpatients,improve the heart function but the risk of hypotension is high. The results should be interpreted carefully.

16.
Article in Chinese | WPRIM | ID: wpr-825620

ABSTRACT

Objective To investigate the effect of compound Ziyin granule (CZG) on sex hormones and oxidative stress levels of ovariectomized rats. Methods The experiment was divided into model group, Liuwei Dihuang pill group, estradiol valerate group, Zishen Yangyin Decoction group, CZG (low, medium, high dose) group, ormal group and sham operation group. After administration for 8 weeks, serum estradiol (E2), follicle stimulation hormone (FSH), luteotropic hormone (LH), total-superoxide dismutase (T-SOD), malonaldehyde (MDA) and glutathione peroxidase (GSH-Px) were measured. Results Compared with the model group, E2 was significantly raised at medium and high doses of CZG, LH was decreased at medium dose, and FSH was reduced at low, medium and high dose; medium and high dose of CZG were capable of remarkably increasing T-SOD, GSH-Px and decreasing MDA, and low dose raised T-SOD and reduced MDA (P<0.05 or P<0.01). Compared with estradiol valerate group, low dose of CZG significantly increased T-SOD (P<0.05). Compared with decoction group, GSH-Px was remarkably raised at medium and high dose (P<0.05). Conclusion The CZG could reverse the abnormal function of sex hormone secretion in the pituitary-ovary axis of the ovariectomy perimenopausal model rat and improve its antioxidant capacity.

17.
Article in Chinese | WPRIM | ID: wpr-825236

ABSTRACT

Objective To analyze the advantages, disadvantages, opportunities and challenges for schistosomiasis elimination in Laos, so as to propose the corresponding healthy policies and suggestions. Methods A SWOT analysis was performed to analyze the strength, weakness, opportunity and threat for the schistosomiasis elimination program in Laos, and the corresponding policy suggestions were proposed. Results The national schistosomiasis elimination program of Laos receives governmental emphases and great supports. A strategy based on mass drug administration was proposed and a sentinel site-bases surveillance system has been built for schistosomiasis elimination in Laos; however, there are several challenges for the national schistosomiasis elimination program in Laos, including insufficient financial supports, inadequate professional capability, weak schistosomiasis control awareness in community populations and difficulty in vector control. Conclusions Persistent governmental leadership, increasing financial supports, strengthening professional team building and improving schistosomiasis control awareness in community populations are required to facilitate the progress towards schistosomiasis elimination in Laos.

18.
Cancer Research and Clinic ; (6): 744-750, 2019.
Article in Chinese | WPRIM | ID: wpr-801624

ABSTRACT

Objective@#To investigate the effect of pelvic radiotherapy on intestinal flora, the correlation between radiation enteritis and intestinal flora, and the effect of probiotics intervention on intestinal flora and diarrhea.@*Methods@#From March to November 2018, a total of 22 patients who were admitted to the Affiliated Suzhou Hospital of Nanjing Medical University were enrolled in the study, including 5 patients with colorectal cancer, 15 patients with cervical cancer and 2 patients with endometrial carcinoma. Five patients with cervical cancer received oral administration of probiotics combined with radiotherapy. Ten healthy physical examiners were selected as the control group. The stool samples were collected from each patient before and after radiotherapy, and 16S rDNA sequencing technology was used to detect the fecal flora and analyze the effect of radiotherapy on intestinal flora.@*Results@#The main composition of intestinal flora in the stool samples of healthy control group was Firmicutes (39.86%), Bacteroidetes (37.77%), unclassified bacteria (15.96%) and Proteobacteria (5.03%), which was similar to patients, but the abundance of bacteria was significantly different. There were differences in the intestinal flora of patients before and after radiotherapy. Microbial diversity and abundance were altered after radiotherapy, especially in patients who later developed severe diarrhea. The Firmicutes/Bacteroidetes ratio was altered after radiotherapy. The abundance of Escherichia-Shigella and Fusobacterium increased after radiotherapy in patients who developed severe diarrhea. Oral administration of probiotics could alleviate the diarrhea and reduce the decline of microbial diversity and abundance.@*Conclusions@#In patients with pelvic tumors, the diversity and abundance of intestinal flora decrease after radiotherapy, especially in patients with severe diarrhea. Oral probiotics can alleviate the diarrhea.

19.
Article in Chinese | WPRIM | ID: wpr-843521

ABSTRACT

Objective: To screen the anti-atherosclerosis (AS) activity of the compounds by using THP-1-HIF-1α-HER-Luciferase high-throughput model, and to verify the anti-AS function of the effective compounds. Methods: THP-1-HIF-1α-HER-Luciferase cells were pretreated with different concentrations of compounds (1, 10, and 100 μg/mL) for 2 h, then cultured under hypoxia for 24 h. Luciferase activity of cells was detected and compounds with anti-AS activity were screened by luciferase activity evaluation. THP-1 and U937 cells were pretreated with effective compounds, and then induced for 24 h by oxidized low density lipoprotein (OX-LDL). The formation of foam cells was observed by oil red staining. The mRNA level of hypoxia-inducible factor 1α (HIF-1α) was detected by real-time quantitative PCR (qPCR). HIF-1α protein expression was detected by Western blotting. Anti-AS activity of effective compounds were evaluated. Results: Among the 200 compounds, 11 compounds could significantly inhibit the increase of luciferase activity in THP-1-HIF-1α-HER-Luciferase cells induced by hypoxia (all P<0.05), and compound numbered 14 (C14) had the most significant inhibitory effect. THP-1 and U937 cells formed foam cells induced for 24 h by OX-LDL. However, cells were pretreated with C14 for 2 h, which could significantly inhibit the formation of foam cells induced by OX-LDL. Cells were induced for 24 h by OX-LDL, which could significantly increase the expression of HIF-1α mRNA and protein (all P<0.05), while cells pretreated with C14 could significantly inhibit the increase of HIF-1α mRNA and protein expression in a gradient-dependent manner (all P<0.05). Conclusion: THP-1-HIF-1α-HER-Luciferase high-throughput model can be reliability used in screening of compounds with anti-AS activity. C14 has the good anti-AS activity characteristics.

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Practical Oncology Journal ; (6): 115-121, 2019.
Article in Chinese | WPRIM | ID: wpr-752824

ABSTRACT

Objective The aim of this study was to investigate the expression of miR-455-5p in epithelial ovarian cancer and its effect on the development of epithelial ovarian cancer. Methods The miRNA expression data of normal ovarian epithelial tis-sues and epithelial ovarian cancer tissues GSE83693 were downloaded from the GEO database. Differential expression analysis was used to obtain differential expression data of miRNAs in epithelial ovarian cancer. The expression of miR-455 -5p was analyzed whether there is difference expression between normal ovarian epithelium and epithelial ovary cancer tissues; qRT-PCR was used to verify the differential expression prediction results; bio-informatics software was used to analyze the KEGG pathway enrichment and GO gene function annotation of miR-455-5p target genes,and to explore the disorders of dyregulated miR-455-5p in the devel-opment of epithelial ovarian cancer. Results A total of 101 cases of differentially expressed miRNAs were screened,34 cases were up-regulated and 67 cases were down-regulated. Among them,miR-455-5p was down-regulated significantly(P<0. 01),and the different fulds were -2. 9019. The results of qRT-PCR showed that the expression of miR-455-5p in epithelial ovarian cancer cells(SKOV-3,OVCAR-3 and A2780)was significantly lower than that in normal ovarian epithelial cells(IOSE-80),and the dif-ferential expression was statistically significant(P<0. 05). The results of KEGG pathway enrichment analysis showed that miR-455-5p regulated target genes mainly involved in five pathways,including TGF-β signaling pathway,Hippo signaling pathway,ECM-receptor interaction,transcriptional dysregulation pathway in cancer,and chronic granule cellular leukemia,which were associated with tumors. GO functional annotation analysis showed that the target genes regulated by miR-455-5p in the above pathway was mainly involved in protein phosphorylation,promoted cell proliferation and migration,inhibited apoptosis,promoted epithelial-mesenchymal transition,regulated transcription and regulated cell cycle,etc. ,which associated with tumorigenesis. Conclusion The expression of miR-455-5p is down-regulated in epithelial ovarian cancer. The miR-455-5p target genes are involved in the pathogenesis and function of epithelial ovarian cancer,and are associated with the development of epithelial ovarian cancer.

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