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Article in Chinese | WPRIM | ID: wpr-254134


<p><b>OBJECTIVE</b>To isolate Japanese encephalitis virus (JEV) from mosquitoes collected in Liaoning province and analysis the genotype of new isolated JEV strains and the characters of nucleotide and amino acid in the E gene.</p><p><b>METHODS</b>Collected mosquitoes in Dandong Liaoning Province in August, 2006. Virus isolation was using issue culture cells. Isolated viruses were identified by using serological and molecular methods.</p><p><b>RESULTS</b>Two new JEV strains, LNDG07-02 and LNDG07-16, were isolated from 1500 mosquitoes were belonging to genotype 1. The identity of nucleotide and amino acid of E gene between new JEV strains and live attenuated vaccine strain SA14-14-2 were 87.8-88% and 97.2%, respectively. Total 11 amino acid sites were differences in E gene between new isolates and SA14-14-2. However, there were no differentiation between the new JEV strains and the isolates in Donggang 2002.</p><p><b>CONCLUSION</b>Genotype 1 JEV was isolated again from Donggang, since the first isolation of this genotype in 2002. Genotype 1 JEV continues in existence in Donggang Liaoning Province.</p>

Amino Acid Sequence , Animals , Brain , Pathology , Virology , Cell Line , China , Epidemiology , Culicidae , Virology , Encephalitis Virus, Japanese , Classification , Genetics , Encephalitis, Japanese , Epidemiology , Virology , Female , Genotype , Insect Vectors , Virology , Male , Mice , Molecular Sequence Data , Phylogeny , RNA, Viral , Genetics , Sequence Analysis, DNA