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Article in Chinese | WPRIM | ID: wpr-755859


Objective To investigate the surgical indication and surgical procedures for Crohn disease.Methods Clinical data of 47 cases with Crohn disease were retrospectively analyzed.Results The main clinical manifestations were abdominal pain (35 cases),diarrhea (16 cases),emaciation and fatigue(12 cases),abdominal mass (9 cases),intestinal obstruction (31 cases),intestinal adhesion (18 cases),intestinal perforation(8 cases),intestinal bleeding (1 1 cases),internal fistula (4 cases),abdominal abscess (4 cases).Preoperative enteroscopy was performed in 23 cases,and 7 cases were diagnosed as Crohn's disease.Operative procedures included colectomy in 15 cases,small bowel resection and intestinal adhesion lysis in 29 cases,ileostomy in 3 cases.Postoperative complications occurred in 13 cases,including incision dehiscence in 2 cases,intestinal fistula in 5 cases,there were 2 cases of stress ulcer,pulmonary infection in 1 case and short bowel syndrome in 1 case,early postoperative inflammatory bowel obstruction in 2 cases and death in 1 case.44 patients were followed-up,for an average of 6.8 years.Recurrence of Crohn's disease was found in 11 cases and canceration in 3 cases.Conclusions Surgery is still the mainstay for Crohn's disease,and close follow-up is important for disease recurrence and canceration.

Acta Pharmaceutica Sinica ; (12): 1613-21, 2015.
Article in English | WPRIM | ID: wpr-505073


Thirteen of 4-anilinoquinazoline derivatives with imine groups at position 6 of quinazoline ring were synthesized and their antitumor activities were evaluated by MTT assay and Western blotting analysis. Among these compounds, 13a-131 were reported first time. The MTT assay was carried out on three human cancer cell lines (A549, HepG2 and SMMC7721) with EGFR highly expressed. Among the tested compounds, 13i and 13j exhibited notable inhibition potency and their IC50 values on three cell lines were equivalent to or less than those of gefitinib. Compound 14, without imine group substituted, displayed excellent inhibitor potency only on A549 cell line. Compounds 14 and 13j were chosen to perform Western blotting analysis on A549. The results showed that both of the compounds could inhibit the expression level of phosphorylated EGFR remarkably. It was concluded that the inhibitor potency of compound 14 was almost equivalent to that of gefitinib and the inhibitor potency of 13j was better than that of gefitinib.

Article in Chinese | WPRIM | ID: wpr-384649


Objective To investigate the effects of evodiamine on autophagy of human colon a cleno carcinoma lovo cells, and to explore the role and mechanism of autophagy which was induced by evodiamine (EVO). Methods MTT assay combined with the morphologic changes were used to observe the cell viability. Monodansylcadaverine was used to detect autophagy by fluorospectrophotometer and the confocal laser fluorescence microscopy respectively. Immunoblotting assay was used to observe the microtubule-associated protein 1 light chain 3. Finally, evodiamine combined with 3-methyladenine to detect the cell viability with MTT assay and the apoptosis with the flow cytometry, respectively.Results Evodiamine inhibited the viability of Lovo cells in dose-dependent manner ( P < 0. 05 ), especially in 60 μmol/L that was obviously(60% ). Further more, the cell lysis and cell gap widened was observed by the light microscope. Evo triggered the autophagy, and after inhibition the autophagy by 3-MA, the killing capacities of the Evo was enhanced ( P < 0. 01 ). However, autophagy prohibited the apoptosis pathways.Conclusions Evodiamine can trigger the autophagy, which might play a self-defense role in evodiamineinduced cell death. The cytototoxicity of evodiamine can be augmented by the autophagy inhibitors. The joint application of autophagy regulators with the chemotherapeutic agents might enhance the cell killing effects of chemotherapeutic drugs and show a potent role in cancer drug resistance.

Article in Chinese | WPRIM | ID: wpr-387932


Objective To evaluate the role of miR-143, miR-145 in the development of gastric gastrointestinal stromal tumor. Methods The expression levels of miR-143 and miR-145 in 21 cases of gastric gastrointestinal stromal tumor and the matched non-tumor adjacent tissue specimens were examined by stem-loop real-time RT-PCR, and its correlation with clinicopathologic features of gastric gastrointestinal stromal tumor were analyzed. Results Expression level of miR-145 were significantly higher in tumor than adjacent normal tissues (P<0.01 ) and that with mitotic count ≥ 5/50HPF cases was significantly lower than that with mitotic count <5/50HPF cases (P=0.02). miR-145 expression in huge tumor (>10 cm)was significantly lower than that in the large tumor (5~10 cm) and small tumor (2~5 cm) (P=0.048).By Fletcher risk stratification system, miR-145 expression in high-risk cases was significantly lower than that in the intermediate-risk and low-risk cases (P=0.048). While the expression of miR-145 in low-risk group was significantly different compared to that in intermediate-risk group and high-risk group (P=0.01).There was no difference between the expressions of miR-143 in tumor and that in normal tissue(P=0.06).Conclusion In gastric gastrointestinal stromal tumor, MiR-145 expression is significantly higher in tumor than adjacent normal tissues. miR-145 is closely associated with tumor size. mitotic counts and Fletcher risk stratification system.

Article in Chinese | WPRIM | ID: wpr-580645


Objective To isolate and determine the structures of chemical constituents from the roots and rootstalks of Rheum emodi.Methods The chemical constituents were isolated and purified by silica gel and polyamide column chromatography.Chemical methods and spectroscopic methods,such as 1H-NMR,13C-NMR,and MS spectra were used for the structure identification.Results Eighteen compounds were obtained.Sixteen of them were identified as:chrysophanol(1),physcion(2),?-sitosterol(3),emodin(4),aloe-emodin(5),rheumin(6),daucosterol(7),d-catechin(8),piceatannol(9),piceatannol-4'-O-?-D-glucopyranoside(10),piceatannol-4'-O-?-D-(6″-O-p-coumaroyl)-glucopyranoside(11),chrysophanol-8-O-?-D-glucopyranoside(12),physcion-1 and 8-O-?-D-glucopyranoside(13a and 13b),emodin-8-O-?-D-glucopyranoside(14),and sucrose(15).Meanwhile,one compound structure simi-lar to rhein was detected.Conclusion Compound 11 is a new compound named rheoside,compounds 13a and 13b are obtained from R.emodi for the first time.