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1.
Mem. Inst. Oswaldo Cruz ; 111(7): 417-422, tab, graf
Article in English | LILACS | ID: lil-787553

ABSTRACT

Yeasts of the genus Candida have high genetic variability and are the most common opportunistic pathogenic fungi in humans. In this study, we evaluated the genetic diversity among 120 isolates of Candida spp. obtained from diabetic patients, kidney transplant recipients and patients without any immune deficiencies from Paraná state, Brazil. The analysis was performed using the ITS1-5.8S-ITS2 region and a partial sequence of 28S rDNA. In the phylogenetic analysis, we observed a consistent separation of the species C. albicans, C. dubliniensis, C. glabrata, C. tropicalis, C. parapsilosis, C. metapsilosis and C. orthopsilosis, however with low intraspecific variability. In the analysis of the C. albicans species, two clades were formed. Clade A included the largest number of isolates (91.2%) and the majority of isolates from GenBank (71.4%). The phylogenetic analysis showed low intraspecific genetic diversity, and the genetic polymorphisms between C. albicans isolates were similar to genetic divergence found in other studies performed with isolates from Brazil. This low genetic diversity of isolates can be explained by the geographic proximity of the patients evaluated. It was observed that yeast colonisation was highest in renal transplant recipients and diabetic patients and that C. albicans was the species most frequently isolated.


Subject(s)
Humans , Male , Female , Candida/genetics , Candidiasis, Invasive/genetics , Diabetes Mellitus/microbiology , Genetic Variation , Kidney Transplantation , Brazil/epidemiology , Candida/classification , Candida/isolation & purification , Candidiasis, Invasive/classification , Candidiasis, Invasive/epidemiology , Candidiasis, Invasive/microbiology , Case-Control Studies , Diabetes Complications , DNA, Fungal/analysis , DNA, Ribosomal/genetics , Microbial Sensitivity Tests
2.
Braz. arch. biol. technol ; 48(1): 1-6, Jan. 2005. ilus, tab
Article in English | LILACS-Express | LILACS | ID: lil-398304

ABSTRACT

Cariótipos de oito linhagens selvagens do fungo entomopatogênico Metarhizium anisopliae var. anisopliae foram obtidos em gel, por eletroforese em campo pulsado. As linhagens foram isoladas de insetos provenientes de seis estados brasileiros. As moléculas de DNA cromossômico de três linhagens foram separadas em sete bandas e, de cinco linhagens, em oito bandas. Polimorfismo de tamanho cromossômico também foi observado. O tamanho do DNA cromossômico de todas as linhagens variou de 7,7 a 0,9 Mb, utilizando-se DNA cromossômico de Aspergillus nidulans como padrão. O tamanho do genoma total foi estimado em pelo menos 29,7 Mb. Algumas correlações entre semelhanças e diferenças no cariótipo eletroforético e a ocorrência do ciclo parassexual como também a especificidade com insetos hospedeiros foram discutidas.

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