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Article in Chinese | WPRIM | ID: wpr-863952


Objective:To investigate the effects of miR-342-3p on proliferation, migration and invasion of thyroid cancer cells and its potential mechanism.Methods:The expression levels of miR-342-3p in human thyroid cancer cell lines (FTC-133, TPC-1, BCPAP and SW1736) and human thyroid follicular epithelial cells (Nthy-ori3-1) were detected by qRT-PCR. Lipofectamine 2000 was used to transfect the FTC-133 cells in the logarithmic growth phase with miR-342-3p mimics (miR-342-3p mimics group) , negative control (miR-NC group) and FTC-133 cells without any transfection (Control group) . qRT-PCR was used to detect the mRNA level of miR-342-3p in each group after transfection to verify the transfection efficiency. CCK-8 assay was used to detect cell proliferation activity. Wound-healing assay and transwell assay were performed to evaluate cell migration and invasion. The dual luciferase reporter assay was used to verify the targeted regulation of Bcl-2 by miR-342-3p. The mRNA and protein expression levels of Bcl-2 were detected by qRT-PCR and Western blot.Results:The expression level of miR-342-3p in thyroid cancer cell lines was significantly lower than that of Nthy-ori3-1 cells ( F=5.732, P=0.011) . Compared with Control and miR-NC groups, the expression level of miR-342-3p was significantly increased ( F=8.613, P=0.003) , the proliferation activity of pcdna3.0-bcl-2 group was significantly increased ( F=11.257, P<0.001) , TP53 protein level was significantly decreased in pcdna3.0-bcl-2 group ( F=9.872, P=0.004) , and the level of TP53 protein in mir-342-3p mimics group was significantly increased ( F=12.548, P<0.001) . Conclusions:miR-342-3p mimics inhibits the proliferation, migration and invasion of FTC-133 cells by targeting and inhibiting Bcl-2 expression, which is expected to be a new target for diagnosis and treatment of thyroid cancer.

China Journal of Endoscopy ; (12): 91-94, 2017.
Article in Chinese | WPRIM | ID: wpr-621125


Objective To discuss the feasibility of single hole thoracoscopy of pleural fibreboard endarterectomy surgical treatment on chronic tuberculous empyema. Methods Retrospective analysis of minimally invasive treatment of 52 cases of chronic tuberculous empyema form January 2013 to May 2016, 50 cases applied single hole thoracoscopy surgery, video-assisted mini-thoracoscopy for another 2 cases. Results There was no death, operation time 60 ~ 240 min, average 160 min, bleeding 150 ~ 2000 ml, average 350 ml, postoperative chest tube drainage time 3 ~ 21 d, average 7 d, postoperative persistent leakage in 3 patients, 3 cases of atelectasis, incisional infection in 1 case, pleural effusion in 1 case, 3 cases of arrhythmia. All the cured patients are received the corresponding treatment, the follow-up of 3 ~ 36 m, the chest CT scan show no atelectasis. Conclusion Under the condition of strict selection of indication, single hole thoracoscopy of pleural fibreboard endarterectomy in treatment of chronic tuberculous empyema is safe and feasible, so it is worthy of making further clinical promotion and application.