Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 136
Filter
1.
Article | IMSEAR | ID: sea-212416

ABSTRACT

Background: Diabetes mellitus is a metabolic disorder of multiple etiology, characterized by chronic hyperglycemia and associated with various microvascular, macrovascular and nonvascular complications leading to increasing morbidity and mortality. Microvascular complications are daibetes specific and their relation with associated comorbidities studied worldwide. To estimate the prevalence of microvascular complications in diabetic patients and to find their relation with various comorbid conditions.Methods: A total of hundred diagnosed patients of diabetes mellitus with age ranging from 18 to 75 years were enrolled in the study. Patients were assessed for the presence of microvascular complications and associated comorbidities. The effect of various comorbidities on frequency of microvascular complications was also determined. Data collected was statistically analyzed.Results: In this study, the mean age of the study group was 55.77±11.75 years. Mean age of onset of the disease was 48.89±11.50 years. Mean duration of the disease in the study group was 6.86±5.02 years. Diabetic retinopathy, neuropathy and nephropathy was found in 76%, 63% and 69% patients, respectively. Among patients with hypertension, 91.7%, 83.3%, and 81.7% cases were found to have retinopathy, neuropathy and nephropathy, respectively. The corresponding figures for those with comorbid IHD were 91.1%, 83.9% and 76.8% respectively whereas among patients with coexisting metabolic syndrome, 92.5%, 80.6%, and 79.1% were found to have retinopathy, neuropathy, and nephropathy, respectively.Conclusions: The most frequent microvascular complication reported by this study was diabetic retinopathy. comorbid conditions like hypertension, ischemic heart disease, and metabolic syndrome were associated with higher prevalence of microvascular complications in diabetic patients.

2.
Article | IMSEAR | ID: sea-214968

ABSTRACT

Birth weight is considered as one of the most important factors that affect the neonatal mortality in both developing and developed countries. In addition, it is a significant determinant of post neonatal mortality, infant and childhood mortality and morbidity. This study was conducted to evaluate the various factors influencing anthropometric measurements of neonates at birth.METHODSThis is a hospital based cross sectional study consisting of neonates and their respective mothers delivered at Darbhanga Medical College, Laheriasarai, Bihar, during the period April 2018 to September 2019. Neonates were divided into three groups < 2500 gms, 2500-3500 gms, >3500 gms and these groups were studied in relation to the determining factors of mothers.RESULTSAmong 1000 babies, 56.3% were male and 43.7% were female. Mean birth weight was 2596.90 ± 542.79 gms ( ± SD). Mean birth weight was higher in males and LBW rate was higher in males. 37.3% of babies were LBW babies. Duration of gestation determines birth weight and other anthropometric parameters proportionately increase with birth weight. In the present study, 15.7% were preterm babies, and 1.8% of babies were post term babies. Factors like maternal age, weight, pregnancy weight gain, socio economic status, Muslim religion, maternal literacy, birth order (2nd onwards), male sex, singleton pregnancy, non-anaemic status, higher period of gestation and spacing had positive correlation with birth weight and negative correlation with LBW incidence. 1/3rd of LBW babies were preterm and 2/3rd were IUGR babies. In this study, anaemia and diabetes was 79.9% and 3% respectively. Maternal diabetes mellitus is an important risk factor for macrosomia.CONCLUSIONSBy avoiding teenage pregnancies, adapting family planning methods, providing good quality antenatal care, improving maternal nutrition, spacing of more than three years, correcting anaemia, early recognition of obstetric complications and timely intervention will reduce percentage of LBW babies, increase the mean birth weight and increase the other newborn anthropometric parameters at birth, which will lower the infant mortality and improve the survival.

3.
Article | IMSEAR | ID: sea-213064

ABSTRACT

Background: Anal fissures are often encountered in surgical practice with surgery the gold standard management for chronic anal fissures. Recently the widespread use of pharmacologic agents for chronic fissures has increased. In our study we compare topical 2% diltiazem with lateral sphincterotomy with respect to symptoms such as relief of pain, ulcer healing, and side effects of treatments.Methods: A prospective comparative study, a total of 80 patients were randomized into 2 groups 40 each. Group A patients were subjected to open internal lateral sphincterotomy and group B to 2% topical diltiazem. The patients in both groups were followed up at 1st, 4th, 14th weeks and 6 months in OPD and were assessed for pain, sphincter tone and complications.Results: In group A (lateral anal sphincterotomy), patients achieved a good pain relief with a mean pain score of 1.98 by one week post procedure whereas group B (2% diltiazem) had taken 14 weeks to achieve similar pain relief (pain score of 1.5). At the end of 6 months, healing of fissure was noted in 100% of group A and in 90% of group B. 4 patients (10%) had recurrences in group B. Flatus incontinence was reported in 2 patients (5%) in group A although transient.Conclusions: Lateral anal sphincterotomy is superior to 2% diltiazem especially in healing of fissure, pain relief, quality of life and recurrence. Pharmacologic agents should be reserved for patients who are unfit or unwilling for surgery or can be used as a bridge therapy till sphincterotomy can be planned.

4.
Article | IMSEAR | ID: sea-210940

ABSTRACT

Infectious diarrhoea in neonates of animals is one of the most common and economically important conditions encountered in the livestock industry. Faecal samples (n=210) from diarrhoeic neonatal goat-kids of different livestock sheds of ICAR-CIRG, Makhdoom, Mathura (U.P.), were aseptically collected, and immediately processed for isolation of bacterial pathogens and parasitic evaluation. A total of 178 isolates of E. coli from 210 samples were identified on the basis of cultural, morphological, biochemical and molecular characteristics. Out of 178 E. coli isolates, 3.93 % (7/178) isolates were identified as STEC by PCR amplification of stx-1 and stx-2 gene. A total of 64 isolates of E. coli were sent to National Salmonella and Escherichia Centre, Central Research Institute, Kasauli for the serotyping. The common serogroups of E. coli responsible for neonatal diarrhoea in goat-kids were identified as O36, O26, O59, O29, O43, O91, O82, O9 and O171, out of which, 46.15% were O36, O26 and O59. Cryptosporidium spp. infection was detected in 46 samples out of 148 faecal samples by ZN staining and nested PCR.Based on cultural, morphological, biochemical and molecular characteristics,16 isolates of Salmonella spp. and 5 of Klebsiella spp. were identified from 210 fecal samples. The present study concluded that E. coli followed by Cryptosporidium spp. and Salmonella spp. were the prevalent infectious agents associated with neonatal diarrhoea in goat-kids

5.
Article | IMSEAR | ID: sea-212839

ABSTRACT

Anal fissures are often encountered in surgical practice in both sexes. It is a distressing disease impacting quality of life and causes profound morbidity among those affected. If left untreated, it may lead onto perianal abscess or even malignancy in long standing cases. Surgery is the gold standard management for chronic anal fissures. Recently the widespread use of pharmacologic agents for chronic fissures has increased. The management of chronic anal fissures has migrated to an era of multifaceted approach. This narrative review looks into various studies spanning over a period of 16 years. Various articles were shortlisted and analyzed for efficacy of various treatment methods, their impact in hospital stay, quality of life improvement, recurrence rate and complications among various treatment methods. We concluded from this review, that open lateral internal sphincterotomy is still the gold standard method of treatment for chronic anal fissure. Among pharmacological agents, 2% diltiazem has the best effectiveness with good compliance rate. Modern surgical techniques like VY plasty can be reserved for special situations. We do not recommend the practice of manual anal dilatation.

6.
Article | IMSEAR | ID: sea-201939

ABSTRACT

Background: Poor sanitation and hygiene are known to be associated with increased morbidity and mortality. Despite all the global efforts 68% population still lacks the basic sanitation facilities. The internal migrant population is a vulnerable group that even lags behind the general population in basic sanitation. This study was aimed to assess sanitation practices among the internal migrant population.Methods: This community based cross sectional study was conducted in a rural village of north-west Delhi from June 2019 to November 2019. People migrated from other parts of the country and residing in the study area for minimum last six months, were included in the study. Data were collected using pre-tested, semi-structured, interviewer-administered study tool, and analysed using SPSS 20.0 software.Results: Two hundred eleven respondents were included in the study. About 88% were using latrine for defecation, out of this 57% were using household sanitary latrines, rest were using community latrines. About 12% were still practicing open defecation. Among those who were using latrine, only about 60% were found regular users. Positive health associated with latrine usage, comfort, privacy, security, presence of latrine in the rented house, quality construction and availability of water were the major motivators and enablers to regular usage of latrine.Conclusions:The internal migrant population is a vulnerable group, with suboptimal sanitation practices. Along with information education communication and behaviour change communication activities, policymakers need to pay special attention to this group in order to achieve global and national sanitation targets.

7.
Article | IMSEAR | ID: sea-212759

ABSTRACT

Background: Hernia is defined as abnormal protrusion of whole or a part of a viscus through the wall that contains it. Among all external abdominal hernias, inguinal hernia is one most commonly encountered. Many factors are responsible for the formation of the inguinal hernia but, what makes a few people more susceptible to this situation is still clearly not proved. The lowness of pubic tubercle is associated with narrow origin of internal oblique muscle from lateral inguinal ligament which fails to protect the deep inguinal ring consequently lead to inguinal hernia.Methods: The study was conducted in Sardar Patel Medical College and attached hospital, Bikaner for duration of 12 months from March 2018 to February 2019. It is a case-control study with 50 cases and 50 control meeting inclusion criteria. In all patients, following parameters SS line, ST line, height, weight was recorded and evaluated.Results: The mean value of ST line in our study group is 7.37±0.182 cm which is significantly greater (p=0.0001) than the controls the mean value being 7.01±0.262 cm. In our study, 98% of cases were having ST line >7.01 cm whereas 66% of controls were ST line under 7.01 cm.Conclusions: Group of people with low lying pubic tubercle are at high risk of developing inguinal hernia.

8.
Article | IMSEAR | ID: sea-203525

ABSTRACT

Background: Carotid Intima Media Thickness (CIMT) andCRP (C Reactive Protein) are have been used for measuringAtherosclerotic Risk in Diabetics. However there is paucity ofdata regarding their importance in Statin Naïve Diabetics.Aim: To study Correlation of CIMT with glycaemic control andhsCRP in Statin Naïve Diabetics.Materials and Methods: 80 Cases (Patient suffering from type2 Diabetes Mellitus) and 20 controls (non- diabetic healthpatients) presenting in outpatient department of a tertiaryHospital were included in this observational cross sectionalobservational study. Complete Blood Count, urine Routine andMicroscopy, urine for microalbuminuria, fasting plasma glucose(FPG), postprandial plasma glucose (PPPG), glycatedhaemoglobin (HbA1C), lipid profile, renal function test, hs-CRPand CIMT were evaluated in each individual. Independentsamples T-test was used to compare CIMT and hs-CRP bothcases and controls. Test of proportion and chi-square test wasused for association between categorical variables.Spearman’s method was used to assess correlation betweenhsCRP and CIMT.Results: 80 cases (Statin naïve and Non-smoker Diabeticpatients and 20 healthy controls were included in this study.Average CIMT in cases was significantly higher in Diabeticcases than Control (0.66 + 0.14 vs 0.56±0.05, difference= 0.10 mm, 95% C.I. (0.1 - 0.17), p < 0.0001). HsCRP wassignificantly correlated with average CIMT in Diabetic cases.(r=0.512, 95% C.I. 0.33-0.658, p<0.0001). Fasting Blood Sugarwas correlated with average CIMT in Diabetic cases. (r=0.234,95% C.I. 0.015-0.432, p=0.0366). Post Prandial Blood Sugarwas also correlated with average CIMT (r=0.300, 95% C.I.0.086-0.488, p=0.00677).Conclusion: Cardiovascular risk factors like glycaemic controland inflammatory markers like hsCRP are significantlyassociated with CIMT even in non-smoking and statin naïveDiabetics.

9.
Article | IMSEAR | ID: sea-201882

ABSTRACT

Background: Adolescence is a stressful period due to physical, psychological, sexual changes and the presence of psychiatric disorders such as depression, anxiety and stress at this stage of life is a matter of concern. Depression is a common mental disorder, characterized by persistent sadness and a loss of interest in activities that you normally enjoy, accompanied by an inability to carry out daily activities, for at least two weeks. The objective of this study is to find the prevalence and associated factors of depression, anxiety and stress among school going adolescents.Methods: This was a school based descriptive cross-sectional study conducted in the month of July 2018 in an urban school in New Delhi. A semi-structured, self-administered questionnaire to assess socio-demographic profile as well as depression anxiety stress scale (DASS)–21 was used to assess depression, stress and anxiety.Results: Overall prevalence of depression, anxiety and stress were found to be 47.9%, 65.3%, and 51.8% respectively. Most of student suffered from moderate depression (46.8%), anxiety (33.3%) and mild stress (60.9%). It was noted that these were more common among female students, late adolescent age group, students alone/ away from family, students from separated/ single parents, consuming alcohol and family pressure to perform well in school.Conclusions: The alarming risk of depression, anxiety and stress among students with low academic satisfaction as well as those who face family pressure to perform better calls for need of parents-student counselling sessions, as well as frequent extracurricular activities to help create a healthier school environment.

10.
Article | IMSEAR | ID: sea-189170

ABSTRACT

Background: Mental health problems contribute significantly to morbidity and mortality across the globe. The impact is more visible in developing countries where less than one in five individuals with psychiatric disorder receives any form of psychiatric help despite immense need. Most of the states in India lack data about the pattern of psychiatric services use in general population thus making it difficult to plan accordingly, The present study was carried out to understand the Sociodemographic profile of inpatient psychiatric services beneficiaries and pattern of psychiatric illnesses among them. Methods: Retrospectively one-year data of admitted patients from July 2017 to June 2018 were extracted manually from the patient files. Results: The study demonstrated that younger individuals were most common recipient of inpatient services followed by middle age patients. Males availed services more frequently and occupied more than 70% of beds at any given time. Schizophrenia (24.8) and bipolar disorders (23.7%) were most common diagnosis and represented almost half of the admitted patients. The other common diagnosis included unspecified psychosis (17.35%), substance use disorders (10.32%) and depressive disorders (4.1%) and dissociative disorders (2.18%). The other categories of diagnosis were barely represented including various childhood and geriatric psychiatric disorders despite the sizeable caseload in community settings. Conclusion: The Sociodemographic profile of psychiatric inpatients was similar to community settings where younger individuals were over represented. Among the patients male preponderance was observed which denotes social and cultural framework of community.

11.
Article | IMSEAR | ID: sea-211733

ABSTRACT

Background: To determine the prevalence of primary drug resistance to either rifampicin or isoniazid alone or both in newly diagnosed sputum smear positive pulmonary tuberculosis patients.Method: A prospective study 100 newly diagnosed sputum smear positive pulmonary TB patients was conducted. The patients with an age of ≥15 years and who had either not taken anti TB treatment or who had taken ATT for less than 1 month were enrolled in this study. Two sputum samples (5ml each), including one early morning sample as per the RNTCP guidelines were collected and subjected to line probe assay (LPA).Results: Out of 100 cases 6 were having resistance to both rifampicin and isoniazid, 9 has resistance to INH alone and 1 had resistance to rifampicin alone.Conclusion: The prevalence of primary drug resistance is high. For early and rapid detection of DR-TB newer modality should be used  for the detection of primary drug resistance in sputum smear positive TB patients.

12.
Article | IMSEAR | ID: sea-207045

ABSTRACT

Background: According to UNICEF, globally 800 million women die due to preventable causes related to pregnancy and childbirth; 20% of which occur in India. It is therefore imperative to understand the level of knowledge about danger signs among pregnant women to augment timely redressal of preventive obstetric causes of mortality.Methods: A hospital based, cross sectional study was conducted at the ANC Clinic in Safdarjung Hospital, New Delhi from 2nd July to 27th July 2018. Convenient sampling was used to identify and interview 354 pregnant women, using a semi structured questionnaire. Data was entered and analysed with SPSSv21. Results were presented as frequencies and proportions. Chi square was used to test for association between qualitative variables, and p-value less than 0.05 was considered significant.Results: 48.3%, 35.6%, and 40.1% of pregnant women had knowledge about danger signs during pregnancy, labour and postpartum respectively. Majority of the women had knowledge about abdominal pain (58.4%) and severe fatigue (80.7%) as danger signs of pregnancy, while bleeding (82.5%) was the most common response as danger sign of labour. More than half had knowledge about heavy bleeding (59.9%) as danger sign of postpartum. The women lacked awareness about Convulsions (92.9%) as danger signs of pregnancy and labour, as well as smelly vaginal discharge (79.6%) in postpartum.Conclusions: Knowledge of obstetric danger signs among pregnant women is still lower. It needs further awareness as it can help in early diagnosis and referral of patients thus reducing maternal mortality and morbidity.

13.
Article | IMSEAR | ID: sea-205113

ABSTRACT

Background/objectives: To assess the quality of life among patients presented with chronic post-stroke patients in our population. Materials and methods: This was a survey type study conducted at physiotherapy department of Liaquat University of Medical and Health Science. Study duration was six months from June 2014 to November 2015. All the patients with the chronic stage of stroke, age 20 to 60 years and patient should not have any disability other than stroke were included study. Patient’s demographic data and information regarding quality of life was assessed by self-maid questioner. Data were analyzed by SPSS version 16.0. Results: Total 100 cases were selected in the study, their mean age was 45.35+3.4 years, most of the cases 73 (73.0%) were with right side affected by stroke. Majority of the cases 47 (47.0%) had acceptable health and 18 (18.0%) patient’s health was poor. 66 (66.0%) patients presented with a history of previous stroke. Most of the patients were moved by help of someone, almost half of patients can move by himself, few patients can’t move and they were completely dependent on others. When patients were interviewed psychologically 41 (41.0%) patients feeling lonely and 31 (31.0%) were depressed and unhappy, 19 (19.0%) had suicidal thought. When patients were interviewed regarding behavior with your family 36 (36.0%) answered as not good behavior of family members, these cases justified that they belong to the poor families and they are as a burden. Conclusion: It was concluded that there was poor quality of life among chronic post-stroke patients especially patients belongs to poor families

14.
Article | IMSEAR | ID: sea-209153

ABSTRACT

Background: This study aims to assess the expression of estrogen receptor alpha (ERα), progesterone receptor A (PRA),Her-2-neu, p53, and Ki-67 in epithelial ovarian tumors and evaluate their correlation with various clinicopathologic variables.Materials and Methods: A total of 50 cases of epithelial ovarian tumors received from the department of obstetrics andgynaecology and surgical oncology were included in this study. Immunohistochemistry (IHC) was performed on sections takenfrom paraffin-embedded tissue blocks. Chi-square test and ANOVA were used for statistical analysis.Results: Among 50 cases of ovarian epithelial tumors, 26 (52%) malignant, 18 (36%) benign, and 6 (12%) borderline. The medianage of patients was higher (53 years) in malignant tumors. ERα had lower expression in benign (27.7%) and PRA had higherexpression in malignant (69%) while Her-2-neu and p53 were negative in benign tumors. ERα and PRA had higher expressionin serous (57.1% and 53.6%), postmenopausal (83.3% and 70%), advanced stage (55.6% and 53.3%), Grade 3 (44.4% and40%), and tumors with ascites (77.8% and 53.3%). Her-2-neu and p53 were negative in benign and higher in malignant (23%and 58%), serous (66.7% and 67%), Grade 3 (57% and 35%), and tumors with ascites (71% and 88%). Ki-67 had a significanthigher expression in malignant (52 ± 28) and Grade 3 tumors (72 ± 20) as compared to benign tumors (4 ± 2).Conclusion: The difference in expression of these markers among benign, borderline, and malignant tumors reveals their rolein differentiation and prognostication of ovarian tumors. Ovarian tumors are extremely heterogeneous as proved by the lackof coexpression of these markers. Tumors with adverse prognostic factors express ERα and PRA; this supports the mitogenicrole of estrogen and estrogenic regulation of PR. Her-2-neu and p53 are expressed only in malignant tumors supporting theirrole in the differentiation of borderline and malignant tumors. Similarly, differential expression of Ki-67 in tumors with adverseprognostic factors would help in prognostication and differentiation.

15.
Article | IMSEAR | ID: sea-203362

ABSTRACT

Background: Basal Metabolic Rate (BMR) represents thelargest component of total energy expenditure and is a majorcontributor to energy balance. Hypertension is an importantglobal health issue and is currently increasing at a rapid pacein most industrializing nations. Hence; under the light of abovementioned data, the present study was planned for assessingthe influence of basal metabolic rate on blood pressure amongadult patients.Materials & Methods: A total of 100 subjects were included inthe present study. Complete demographic details of all thesubjects were obtained. Assessment of smoking status,residence details and past medical history was done.Hemodynamic parameters of all the patients were recorded.Weight and height of all the patients was calculated. BMR wasassessed on the basis of these parameters.Results: Mean age of the patients of the present study was53.4 years. 60 patients in the present study were males whilethe remaining 40 patients were females. Significant correlationwas observed in between BMR and blood pressure.

16.
Indian Pediatr ; 2019 Apr; 56(4): 311-313
Article | IMSEAR | ID: sea-199310

ABSTRACT

Objectives: To determine the diagnostic accuracy of polymerase chain reaction-baseddetection of sof gene compared to throat swab culture for S. pyogenes infection in patientswith acute rheumatic fever and those with recurrence of rheumatic activity. Methods: 40patients between 3 to 18 years of age, with clinical diagnosis of acute rheumatic fever or newactivity in established rheumatic heart disease were included. The amplicon of 228bp of sofgene was detected using a polymerase chain reaction-based technique and the results werecompared with throat swab culture for Streptococcus pyogenes. Results: 10 patients hada positive throat swab culture and 11 had sof gene detected. The sensitivity and specificityof the test was 100% and 96.7%, respectively compared to throat swab culture (P=0.001).The positive predictive value and the negative predictive value was 90.9% and 100%respectively. Conclusions: Polymerase chain reaction-based detection of sof geneprovides an alternative to throat swab culture in diagnosing activity in Acute RheumaticFever or established Rheumatic heart disease.

17.
Article | IMSEAR | ID: sea-210823

ABSTRACT

Eight clinical cases of dogs having femur fracture, were divided into two groups, A and B with four dogs (n=4) in each group. Group A animals were treated with intramedullary pinning (IMP) alone and group B were treated using IMP alongwith demineralized bone matrix (DBM) implantation at the fracture site. The efficacy of healing was evaluated on the basis of clinical evaluation, haemato-biochemical and radiographic parameters on the day of admission (day 0), followed by 7th, 15th, 30th, 45th and 60th post-operative days. The weight bearing was observed to be better in dogs of group A. Swelling was completely absent after 15th post-operative day in dogs of both the groups. The mean values of Hb, PCV, TLC and DLC showed a non-significant variation on subsequent post-operative days in both the groups. There was a significant increase in serum calcium up to 30th post-operative day and thereafter, followed a decreasing trend. The serum alkaline phosphatase values showed non-significant variation in group A while in group B animals significant increase observed on 15th, 30th and 45th post-operative day. Radiographically, the dogs of group B showed better radiographic union of fracture evidenced by early disappearance of fracture line than those of group A

18.
Article | IMSEAR | ID: sea-188757

ABSTRACT

Dyslipidemia and raised serum hsCRP level occurring in diabetic patients, play an important role in development of macrovascular and microvascular complications as compared to nondiabetics. Keeping in view the prevalence and increased risk of multiorgan involvement in diabetes, it is imperative to diagnose prediabetic individuals and assess their serum lipid profile and serum hsCRP level and Use of these biomarkers as a preventive measure from the complications of prediabetes, developing overt diabetes and increased morbidity and mortality. Aim: observation of serum lipid profile and hsCRP in prediabetic population. Methods: Prospective cross-sectional case control observational studycarried out between august 2017- November 2018 (16 months) which included 100 cases (prediabetics) and 100 controls (nondiabetics). Lipid profile and serum hsCRP level of all prediabetic individuals and nondiabetics was done and statistically analyzed. Results: Serum Total cholesterol, triglyceride (TG), low density lipoprotein (LDL) and serum hsCRP level were significantly raised in prediabetic individuals as compared to nondiabetics, whereas high density lipoprotein (HDL) was nonsignificantly lower and mean with SD was much lower side in prediabetic individuals as compared to nondiabetics. Conclusion: Prediabetics had altered lipid profile as compared to nondiabetics. These prediabetic individuals, because of their raised hsCRP level and dyslipidemia, are at higher risk for developing cardiovascular disease and other multiorgan involvement. So to halt progression by primary prevention as Lifestyle modification or pharmacotherapy in such individuals becomes animportant public health consideration.

19.
J. Morphol. Sci ; 36(1): 17-23, March 2019.
Article in English | LILACS-Express | LILACS | ID: biblio-1046991

ABSTRACT

Introduction Type 1 diabetes is an autoimmune disorder characterized by lack of insulin production by the ß cells of the pancreas. This lack of insulin causes a variety of systemic effects on the metabolism of the body, one of which is reproductive dysfunction. The present study investigates the effects of diabetes on the male reproductive system of streptozotocin (STZ)-induced diabetic rats. Material and Methods A total of 18 adult male Wistar rats weighing between 250 and 300 g were included in the present study. The animals were divided into normal and diabetic groups. The diabetic group was further subdivided into 2 subgroups with durations of 24 and 48 days. A single dose of STZ (40 mg/kg body weight) was administrated intraperitoneally to the animals of the diabetic group. After the planned duration, the testes and epididymides were dissected, and their gross weight was measured. The tissues were then processed for histological study. Results The gross weight of the testes and epididymides in diabetic rats at 24 and 48 days showed a decrease in comparison to the control. (p < 0.01 for testes and epididymides). Diabetic animals presented a significant decrease in the diameter of the seminiferous tubules compared with the control group (p < 0.01). The epididymides in the diabetic groups showed a considerable reduction in the tubular surface area compared with the control group (p < 0.01). There was also a reduction in the mean diameter, which was measured using the maximum and minimum diameter of the tubules (p < 0.01). Conclusion The present study is an insight into the adverse effects that diabetes can have on the tissue structure of the testes, of the epididymides, and ultimately on the process of spermatogenesis.

20.
Article | IMSEAR | ID: sea-190089

ABSTRACT

Black gram (Vigna mungo L.) is waterlogging sensitive legume crop. We studied the effect of waterlogging stress on membrane stability index (MSI), lipid peroxidation, superoxide dismutase (SOD) activity, chlorophyll content and chlorophyll fluorescence in four Vigna genotypes namely (Uttara, T-44, IC530491, IC519330). Stress was imposed for 10 days at vegetative stage (30 days after sowing). Thereafter, excess water was drained to allow recovery in stressed plants. Waterlogging treatment significantly increased lipid peroxidation and SOD activity in all the genotypes, which showed the oxidative injury posed by stress conditions. Chlorophyll content and fluorescence reduced under stress conditions. SOD activity, MSI and chlorophyll content was more in IC530491 and IC519330, T44 as compared to Uttara. Lipid peroxidation was high in Uttara. Though chlorophyll fluorescence reduced in all the genotypes under waterlogging, genotypic differences were non-significant. More efficient antioxidative scavenging to maintain membrane stability and chlorophyll content in black gram was found to be associated with tolerance to waterlogging.

SELECTION OF CITATIONS
SEARCH DETAIL