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1.
Indian J Exp Biol ; 1992 Feb; 30(2): 99-102
Article in English | IMSEAR | ID: sea-58961

ABSTRACT

Modification of A. conoides beta-glucosidase by diethylpyrocarbonate caused rapid inactivation of the enzyme. The kinetic analyses showed that the inactivation by diethylpyrocarbonate resulted from the modification of an average of one histidine residue per mole of enzyme. The modified enzyme showed an increase in absorbance at 240 nm. Sulphydryl, lysine and tyrosine residues were not modified by diethylpyrocarbonate treatment. The substrate offered significant protection against diethylpyrocarbonates modification. The results indicate that diethylpyrocarbonate was interacting with the enzyme at or near the active site.


Subject(s)
Binding Sites , Diethyl Pyrocarbonate/pharmacology , Histidine/physiology , Iodoacetamide/pharmacology , Mitosporic Fungi/enzymology , Nitrophenylgalactosides/pharmacology , Pyridoxal Phosphate/pharmacology , beta-Glucosidase/drug effects
2.
Indian J Biochem Biophys ; 1990 Jun; 27(3): 146-50
Article in English | IMSEAR | ID: sea-28704

ABSTRACT

Two endoglucanases, designated Endo I and Endo II, were purified from the culture filtrates of a nematode trapping fungus, Arthrobotrys oligospora. The purification procedure entailed ammonium sulphate precipitation, gel filtration and preparative PAGE. Both the preparations (Endo I and Endo II) were homogeneous by PAGE, had molecular weights of 24,300 and 44,500 respectively as determined by non-denaturing PAGE, and yielded only cellobiose as the main product of CM-cellulose hydrolysis. The optimum pH and temperature for Endo I were 6.0 and 50 degrees C, and for Endo II, pH 5.6-6.4 and 50 degrees C. The two enzymes differed with respect to their Km (Endo I, 5.04 mg/ml; Endo II, 3.2 mg/ml) and energy of activation values (Endo I, 10.7 kCal; Endo II, 9.5 k Cal). Both enzymes were completely inhibited by 1.25 mH Hg2+ and partially by Pb2+, DTNB and p-HMB while DTT and GSH enhanced their activities.


Subject(s)
Catalysis , Cellulase/isolation & purification , Mitosporic Fungi/enzymology
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