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1.
Chinese Journal of Immunology ; (12): 393-397, 2018.
Article in Chinese | WPRIM | ID: wpr-702740

ABSTRACT

Objective:To investigate the role of IL-17D to regulate the recruitment of lung NK cells and the effects of Astragalus membranaceus on IL-17D.Methods:The lung metastasis of tumor model was established by injecting B16 melanoma cells through caudal vein.After being treated with IL-17D or Astragalus membranaceus respectively,the expression of IL-17D and NK cells in lung was detected by flow cytometry and RT-PCR.Results:The production of IL-17D and the content of NK cells in the lung tumor me-tastasis model were dramatically decreased.After treatment with IL-17D,the content of NK cells in lung was significantly increased and the lung tumor foci were decreased.Along with up-regulated recruitments of NK cells,CXCL9 and IL-15,which support NK cell recruitments and maintain survival,were significantly increased.Astragalus inhibited lung tumor developments in lung by increasing the IL-17D expression and up-regulated the lung NK cell content.Conclusion: IL-17D can regulate the lung recruitment of NK cells.Astragalus mongholicus can enhance the capacity of NK cell recruitment and promote anti-tumor immune effects by up-regulating the expression of IL-17D.

2.
Chinese Journal of Neuromedicine ; (12): 878-883, 2016.
Article in Chinese | WPRIM | ID: wpr-1034446

ABSTRACT

Objective To explore the experimental methods and conditions of 131I-labeled anti-epidermal growth factor receptor (EGFR) vⅢ preparation,and to evaluate the targeting distribution of 131I-Anti-EGFRvⅢ in malignant glioma-loading nude mice.Methods The 131I labeling on anti-EGFRvⅢ was performed by Iodogen method.The labeling rate was determined after separation and purification and paper chromatography was used for the determination of radioactive chemical purity.Twenty-eight U87-EGFRvⅢ malignant glioma-loading nude mice with glioma average diameter of 10-15 mm were chosen and randomly divided into group of 131I-Anti-EGFRvⅢ intravenous injection,group of Na131I intravenous injection,group of 131I-Anti-EGFRvⅢ intratumor injection and group of Na131I intratumor injection;7.5 MBq/0.1 mL labeled products with 131I-Anti-EGFRvⅢ or Na131I were injected in the veins or the tumors to observe the changes of the radioactivity distribution of malignant glioma-loading nude mice with SPECT imaging.Results The rate of 131I-labeled anti-EGFRvⅢ was (68.12±6.19)%,and the immediate rate of radiochemieal purity was (95.12±0.59)%,and (87.78 ±5.35)% in room temperature and (85.12±3.58)% in 37 ℃ serum placed for 24 h.SPECT scan showed that the tumor site had significantly stronger imaging than the thyroid gland with the labeled products either by intravenous or intratumor injection.Conclusions It is applicable to the 131I-labeled Anti-EGFRvⅢ with Iodogen method.131I-Anti-EGFRvⅢ has good radiation chemical purity and stability in vitro and in vivo,and could be combined with tumor tissue specificity.

3.
Article in English | WPRIM | ID: wpr-56427

ABSTRACT

Molecular mechanisms underlying the effects of Fyn on cell morphology, pseudopodium movement, and cell migration were investigated. The Fyn gene was subcloned into pEGFP-N1 to produce pEGFP-N1-Fyn. Chinese hamster ovary (CHO) cells were transfected with pEGFP-N1-Fyn. The expression of Fyn mRNA and proteins was monitored by reverse transcription-PCR and Western blotting. Additionally, transfected cells were stained with 4',6-diamidino-2-phenylindole and a series of time-lapse images was taken. Sequences of the recombinant plasmids pMD18-T-Fyn and pEGFP-N1-Fyn were confirmed by sequence identification using National Center for Biotechnology Information in USA, and Fyn expression was detected by RT-PCR and Western blotting. The morphology of CHO cells transfected with the recombinant vector was significantly altered. Fyn expression induced filopodia and lamellipodia formation. Based on these results, we concluded that overexpression of mouse Fyn induces the formation of filopodia and lamellipodia in CHO cells, and promotes cell movement.


Subject(s)
Animals , Cricetinae , Mice , Blotting, Western , CHO Cells , Cricetulus , Genetic Vectors , Green Fluorescent Proteins/genetics , Proto-Oncogene Proteins c-fyn/genetics , Pseudopodia/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Time-Lapse Imaging , Transfection
4.
Military Medical Sciences ; (12): 908-911,917, 2014.
Article in Chinese | WPRIM | ID: wpr-600045

ABSTRACT

Depression is a serious mental disorder characterized by lasting anhedonia and anorexia .The pathophysiology of depression is complicated , which is related to many neuroendocrine disturbances .Increased levels of glucocorticoid hor-mones and hyperactivity of the hypothalamus pituitary adrenal ( HPA) axis are the most consistent and typical pathophysio-logical alternations in patients with major depression , which are possibly caused by altered functions of the receptor of glu-cocorticoid hormones , the glucocorticoid receptor ( GR) .Promoting the expression and function of GR and restoring the im-paired feedback inhibition of the HPA axis seem to be particularly important for the therapeutic efficacy of antidepressants . In this review, the role of GR in the development and resolution of depression is discussed .

5.
Article in English | WPRIM | ID: wpr-194863

ABSTRACT

Kinetin (Kn) is a cytokinin growth factor that exerts several anti-aging and antioxidant effects on cells and organs. To investigate the mechanism underlying apoptotic events in aging cells induced by D-galactose (D-gal), we examined the effect of Kn delivered via nuchal subcutaneous injection on D-gal-induced aging and apoptosis in rats. Our results showed that interleukin (IL)-2 levels and mitochondrial membrane potential (DeltaPsim) were decreased by Kn in aging rats while IL-6 production and apoptosis increased. In addition, the expression of anti-apoptotic Bcl-2 was low while that of Bax was high in the aging group. After treated with Kn, compared with aging group, there showed obvious difference in Kn group with elevated IL-2, proliferation index, Bcl-2, DeltaPsim and decreased IL-6 and Bax in splenic lymphocyte. Based on these results, we concluded that Kn can effectively protect the rat spleen from aging, apoptosis, and atrophy.


Subject(s)
Animals , Female , Male , Rats , Aging/drug effects , Apoptosis/drug effects , Galactose/pharmacology , Interleukin-6/physiology , Interleukins/physiology , Kinetin/pharmacology , Membrane Potential, Mitochondrial/drug effects , Spleen/cytology
6.
Chinese Journal of Neuromedicine ; (12): 676-680, 2012.
Article in Chinese | WPRIM | ID: wpr-1033569

ABSTRACT

[Objective]To study the intervention effect of mild hypotherrnia on cerebral edema in rats after cerebral infarction.[Methods]Twelve male SD rats were randomized into control group (n--6) and mild hypotherma treatment group (n=6).The rat models of focal cerebral infarction were established with obstructing the middle cerebral artery of rats by suture emboli.Rats of the mild hypotherma treatment group were treated by mild hypotherm for 3 h after the models were established.MRI was performed at 6 h,and 1,3,5 and 7 d after the success of model making.Cerebral infarction volume,absorption rate of edema,and signal intensity ratios (SIRs) ofTIWl,T2WI,FLAIR and DWI sequencas in the infarction zones,and relative variance ratio of SIR (△SIR) were measured and calculated.[Results] In the mild hypotherma treatment group,the cerebral infarction volumes 3,5 and 7 d after the models were established were significantly decreased as compared with those in the control group (P<0.05);the absorption rate of edema (the 7th d against the 3th d of infarction) in the the mild hypotherma treatment group was significantly higher than that in the control group (P<0.05).Three,5 and 7 d after infarction,the SIRs of TIWI were all obviously higher than those in the control group,and the SIRs of T2WI and FLAIR were all significantly lower than those in the control group (P<0.05).The SIR of DWI in the mild hypotherma treatment group was obviously lower than that in the control group 7 d after infarction (P<0.05).The △SIRs of T2Wl,FLAIR and DWI in the mild hypotherma treatment group were significantly higher than those in the control group (P<0.05).[Conclusion] Mild hypothermia has a significant inhibition effect on cerebral edema in rats after cerebral infarction.

7.
Chinese Journal of Neuromedicine ; (12): 1106-1109, 2012.
Article in Chinese | WPRIM | ID: wpr-1033658

ABSTRACT

Objective To analyze the risk factors of vascular cognitive impairment in patients after stroke at early stage,Methods Three hundred patients with stroke at early stage were chosen in our study; their age,gender,levels of blood pressure,lipids and HbA1c,homocysteine (Hcy),and high-sensitivity C-reactive protein (HS-CRP),atrial fibrillation,smoking,drinking and lesion sites were detected.Based on the scores in Montreal Cognitive Scale 30 d after the onset,statistical analysis was performed on the above factors.Results Univariate analysis showed that age,atrial fibrillation,levels of HbA1c and blood pressure,lesion sites,HS-CRP level,and Hcy level were significantly associated with the vascular cognitive impairment.Further logistic regression analysis indicated that levels of HbA1c and blood pressure,and lesion sites,and HS-CRP and Hcy levels were the independent risk factors (OR=1.489,P=0.021,95%CI:1.054~1.956; OR=1.134,P=0.027,95%CI:1.011~1.130; OR=3.124,P=0.028,95%CI:1.167~3.012; OR=2.476,P=0.029,95%CI:1.897~2.245; OR=2.132,P=0.032,95%CI:1.097~2.323).Conclusion Levels of HbA1c and blood pressure,and lesion sites,and HS-CRP and Hcy levels can serve as the predictive indexes of vascular cognitive impairment in patients after stroke at early stage.

8.
Acta Physiologica Sinica ; (6): 238-244, 2012.
Article in Chinese | WPRIM | ID: wpr-335917

ABSTRACT

In recent years there have been more widely and deeply studies in investigating melamine toxicity. Generally, it is believed that the main target of melamine is the urinary system. However, previous studies revealed that it also had additional biological actions. Obviously, the toxicity mechanisms of melamine have not been fully clarified. It is well known that fetus and infant periods play the most fundamental role in the brain development. And melamine can pass through the placental and blood-brain barrier, and then exerts toxic effects on the central nervous system. This article reviewed the reports about the topic in recent years, for better understanding the dangers of melamine to infants and providing experimental data for further study.


Subject(s)
Animals , Female , Humans , Pregnancy , Blood-Brain Barrier , Brain , Central Nervous System , Cognition , Maternal-Fetal Exchange , Triazines , Pharmacokinetics , Toxicity
9.
Article in Chinese | WPRIM | ID: wpr-407473

ABSTRACT

AIM Xiaobuxin-Tang (XBXT) is a traditional Chinese herbal decoction which is composed of Haematitum, Flos Inulae, Folium Phyllostachydis Henonis and Semen Sojae Preparatum. The present study was to investigate if the total flavonoids extracted from XBXT (XBXT-2) had antidepressant effect. METHODS Forced swimming tests in mice and rats, and learned helplessness (LH) model of rats were adopted to affirm the antidepressant effect of XBXT-2 with the test on spontaneous motor activity. Plasma corticosterone level in the LH rats was measured with ELISA. RESULTS Single administraton of XBXT-2 at the doses of 50 and 100 mg·kg-1 (ig) significantly decreased the duration of immobility time in the forced swimming tests in mice and rats. Researches on LH model of rats indicated that XBXT-2 at doses of 50 and 25 mg·kg-1 markedly reduced the number of escape failure in shuttle box. Meanwhile, the plasma corticosterone level of the LH rats was significantly decreased. XBXT-2 50-200 mg·kg-1 had no effects on spontaneous motor activity in mice. CONCLUSION XBXT-2 possesses significant antidepressant-like effect. The mechanism may involve the inhibition of the hyperaction of the hypothalamic-pituitary-adrenal axis.

10.
Article in Chinese | WPRIM | ID: wpr-356741

ABSTRACT

This paper gave a brief introduction of the effect of Solanum nigrum on anti-cancer. The experimental results showed that the total alkaloid isolated from S. nigrum interfered structure and function of tumor cell membrane, disturbed the synthesis of DNA and RNA, changed the cell cycle distribution, so that total alkaloids could play in inhibabition to tumor cells, while the glycoprotein (150 x 10(3)) isolated from S. nigrum might have shown anti-cancer abilities by blocking the anti-apoptotic pathway of NF-kappaB, activating caspase cascades reaction and increasing the production of nitric oxide.


Subject(s)
Animals , Humans , Alkaloids , Pharmacology , Antineoplastic Agents, Phytogenic , Pharmacology , Apoptosis , Caspase 3 , Metabolism , Cell Line, Tumor , Cell Membrane Permeability , DNA, Neoplasm , Drugs, Chinese Herbal , Pharmacology , Glycoproteins , Pharmacology , NF-kappa B , Metabolism , Nitric Oxide , Metabolism , Plants, Medicinal , Chemistry , RNA, Neoplasm , Sialic Acids , Metabolism , Solanum nigrum , Chemistry
11.
Article in Chinese | WPRIM | ID: wpr-683075

ABSTRACT

Objective To study the effect of cardiac troponin T(cTnT)on the prognosis of chronic heart failure in the elderly patients.Methods Serum level of cTnT,and serum activities of creatine kinase (CK)and creatine kinase-MB(CK-MB)were measured in 60 elderly patients of chronic heart failure with varied classes of cardiac function.Cardiac events,including malignant cardiac arrhythemia,repeated or aggravated heart failure or cardiac death,in patients with increased cTnT during hospitalization were compared to those without it.Results Serum level averaged(89.1?13.6)mmol/L for CK and(7.9? 1.4)mmol/L for CK-MB in 20 patients with Class Ⅱ cardiac function by New York Heart Association (NYHA)classification and without increased cTnT,(88.2?13.4)mmol/L for CK and(7.5?1.8) mmol/L for CK-MB in 19 patients with Class Ⅲ cardiac function and four of them with increased cTnT,and (93.3?14.2)mmol/L for CK and(8.1?1.6)mmol/L for CK-MB in 21 patients with Class Ⅳ cardiac function and 14 of them with increased cTnT.Malignant cardiac arrhythemia occurred in six,repeated heart failure in eight,and death in three of 18 patients with increased cTnT,and one,two and one in 22 patients without increased cTnT,respectively.Incidence of cardiac events was higher in patients with increased cTnT than those without it(P

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