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1.
Article in Chinese | WPRIM | ID: wpr-880093

ABSTRACT

OBJECTIVE@#To investigate the significance of low-density lipoprotein receptor-related protein 5 and 6 (LRP5/6) in the Wnt/β-catenin signaling pathway in the pathogenesis and prognosis of childhood acute lymphoblastic leukemia (ALL).@*METHODS@#A total of 43 children who were newly diagnosed and achieved complete remission after remission induction therapy were enrolled. The children before treatment were included in incipient group, and after treatment when achieved complete remission included in remission group. A total of 39 children with immune thrombocytopenia were enrolled in control group. Three milliliter bone marrow samples were collected from above-mentioned each group. QRT-PCR was used to determine the mRNA expression of LRP5 and LRP6 in blood mononuclear cells of bone marrow. Western blot was used to detect the protein expression of LRP5 and LRP6. According to the protein expression levels of LRP5 and LRP6, the children were divided into low-expression group and high-expression group, and the clinical biological characteristics were compared between these two groups. Survival analysis was performed by Kaplan-Meier method.@*RESULTS@#Both mRNA and protein expression levels of LRP5 and 6 were upregulated in the incipient group compared with the control and remission group (P<0.05). The mRNA and protein expressions of LRP5 and LRP6 in the high-risk group were higher than those in the medium-risk group (P<0.05), it is the same as in the medium-risk group than the low-risk group (P<0.05). The mRNA and protein expressions of LRP5 and 6 positively correlated with risk degree in the incipient group (r@*CONCLUSION@#The high expression of LRP5/6 may be one of the pathogenesis of childhood ALL, and the degree of LRP5/6 increase may be related to the risk level.


Subject(s)
Child , Humans , Lipoproteins, LDL , Low Density Lipoprotein Receptor-Related Protein-5 , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Receptors, LDL , Wnt Signaling Pathway , beta Catenin/metabolism
2.
Article in Chinese | WPRIM | ID: wpr-873103

ABSTRACT

Objective::To study the effect of plant-soil feedback on the antioxidant enzyme system of Acanthopanax senticosus seedlings, in order to elucidate the changes of plant-soil feedback on the antioxidant enzyme system of A. senticosus seedlings, and provide theoretical basis for revealing the reasons of plant-soil feedback. Method::Through the greenhouse pot experiment, plant height, leaf color value (SPAD), antioxidant enzymes [protein, superoxide dismutase(SOD), catalase(CAT), peroxidase(POD), aseorbateperoxidase(APX), malondialdehyde(MDA)] and yield and growth related indexes of soil without A. senticosus (group 1), soil with A. senticosus (group 2) for three consecutive years and soil with A. senticosus (group 3) for many years were measured respectively. Result::There were significant differences in plant height, SPAD, protein, SOD, CAT, POD, APX and MDA between seedlings of A. senticosus planted for three consecutive years (group 1), two successive years (group 2) and three successive years (group 3). The biomass, MDA, CAT, POD and SOD of A. senticosus seedlings in the soil without A. senticosus (group 1) were higher than those in the soil with A. senticosus (group 2 and 3), while the protein and APX were lower than those in the soil with A. senticosus (group 2 and 3). Conclusion::Plant and soil shows negative feedback regulation during the growth of A. senticosus seedlings, which reduced the activity of antioxidant enzymes. Therefore, the soil without planting A. senticosus has more advantages for the growth of A. senticosus seedlings. The results provide a basis for explaining the effect of plant-soil feedback on the growth of A. senticosus, and a theoretical basis and technical support for the technical standards of A. senticosus cultivation in farmland.

3.
Article in Chinese | WPRIM | ID: wpr-873102

ABSTRACT

Objective::To study the effect of different N application rates on the growth and development of Acanthopanax senticosus and the changes of antioxidant enzyme activity, and screen out the suitable amount of nitrogen fertilizer for its growth and development, in order to provide scientific evidence for rational fertilization of Acanthopanax senticosus in artificial cultivation. Method::By single-point, single-factor field experiment, the study samples were one-year-old seedlings of growing evenly A. senticosus.Five nitrogen application treatment groups were set up in the fields.They were N1 (30 g·m-2), N2 (60 g·m-2), N3 (90 g·m-2), N4 (120 g·m-2), N5 (150 g·m-2) and CK (0 g·m-2) in the control group.Three months later, the raw weight of plant, root, leaf and stem were measured at harvest time.After drying to constant weight, plant dry weight, stem dry weight, leaf dry weight and root dry weight were measured.Fresh leaves of plants were collected to measure malondialdehyde(MDA) content and activities of catalase(CAT), superoxide dismutase(SOD), peroxidase(POD), acid phosphatase(ACP) and aseorbateperoxidase(APX) after harvesting seedlings. Result::The biomass of A. senticosus in group N4 (120 g·m-2) was the highest, the protein content in group N3 (90 g·m-2) was the highest, and the activity of all antioxidant enzymes in group N3 (90 g·m-2) was the lowest. Conclusion::There is a dose-effect relationship between seedlings and the nitrogen application rate.That is to say, low nitrogen application rate and high nitrogen application rate will cause stress on Acanthopanax senticosus, and the activity of antioxidant enzymes under low nitrogen application rate is higher than that under high nitrogen application rate.

4.
Article in Chinese | WPRIM | ID: wpr-873101

ABSTRACT

Objective::To study the effect of different nitrogen application rates on the growth and carbon metabolism of Acanthopanax senticosus seedlings, and screen out the rational fertilization conditions, in order to provide basis and guidance for scientific fertilization of artificially cultivated A. senticosus. Method::A single-point, single-factor field experiment was conducted to study the seedlings of growing evenly A. senticosus.Five different nitrogen application treatment groups were set up to treat the seedlings, namely N1 group (30 g·m-2), N2 group (60 g·m-2), N3 group (90 g·m-2), N4 group (120 g·m-2), N5 group (150 g·m-2) and CK group (0 g·m-2), respectively.Three months later, plant height, root circumference, stem circumference, root-shoot ratio and SPAD were measured at harvest time.The contents of protein, sucrose, starch, soluble sugar and reducing sugar in fresh leaves were measured. Result::N3 treatment was the best treatment method for the growth and development of A. senticosus seedlings, and the growth of A. senticosus seedlings was the best under this treatment.The protein content of A. senticosus seedlings in N3 treatment was the highest.Starch and sucrose were best accumulated in N5 treatment group and CK treatment group.N5 treatment had the highest soluble sugar content and reducing sugar content. Conclusion::There is a dose-effect relationship between the growth and development of A. senticosus seedlings; that is to say, low and high nitrogen application treatments will cause stress on A. senticosus seedlings.In conclusion, the suitable nitrogen application rate for A. senticosus growth is 90-120 g·m-2.

5.
Article in Chinese | WPRIM | ID: wpr-873100

ABSTRACT

Objective::To study the correlation between endogenous hormone content, related enzyme activity and embryogenesis during the stratification of Acanthopanax senticosus seeds, and to provide theoretical basis for application of endogenous hormones and related physiological indicators in regulating germination of A. senticosus seeds in production and scientific research. Method::Endophytic fungi as well as different concentrations of nitric oxide and hydrogen peroxide solution were used for soaking the seeds of A. senticosus, and then thermophilic stratification was conducted for the seeds. The content of endogenous hormones such as gibberellin (GA3), abscisic acid (ABA), indole acetic acid (IAA), indole butyric acid (IBA) and salicylic acid (SA) in seeds of A. senticosus were tested by high performance liquid chromatography (HPLC), and the activity change of enzymes in vivo such as catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and malondialdehyde (MDA) were tested. The correlation between the embryo rate and the hormones and their enzyme activities in the seeds of A. senticosus was studied by the grey correlation method. Result::The embryo rate was significantly positively correlated with IAA, GA3, and CAT, with correlation coefficient of 1.086, 0.935 and 1.067, respectively. There was a significant positive correlation between IAA, GA3, CAT, IBA, and SA, but with a significant negative correlation with ABA, MDA, and POD. The correlation degree was as follows: IAA>CAT>GA3>IBA>SA>SOD>ABA>POD>MDA. Conclusion::IAA, GA3 and CAT have significant promoting effects on embryo rate, and there is a significant correlation between hormone content and enzyme activity, which provides a basis for exploring the seed germination mechanism of A. senticosus.

6.
Article in Chinese | WPRIM | ID: wpr-872837

ABSTRACT

Objective:To study the effect of different shading conditions on the growth and photosynthetic physiological parameters of Acanthopanax senticosus,and provide a theoretical basis for standardized planting and rational development and utilization of A. senticosus. Method:Three-year-old A. senticosus was used as the experimental sample.The growth parameters and photosynthetic physiological parameters of plant height,leaf number and leaf area were determined to study the effects of different shading conditions on the growth and photosynthesis of A. senticosus. Result:Plant height,leaf number,and leaf area were significantly higher in the shading treatment than in the control group,and highest under moderate shading conditions.The net photosynthetic rate,stomatal conductance,and transpiration rate were significantly higher in the moderate shading group than in the control group,and decreased in the severe shading group,while the intercellular carbon dioxide concentration was significantly lower in the moderate shading group than in the other treatment groups.As the treatment time progressed,the initial fluorescence was not significantly higher in the moderate shading group than in the control group,but significantly higher in the severe shading group;the maximum fluorescence was significantly higher in the shading group than in the control group,in the moderate shading group.The potential photochemical efficiency and maximum photochemical efficiency were not significantly different between the moderate shading group and the control group and decreased in the severe shading group,which was significantly lower than other treatment groups. Conclusion:Shading treatment is beneficial to the growth of A. senticosus.The moderate shading condition can significantly improve the photosynthesis of A. senticosus.Severe shading treatment can significantly inhibit the photosynthesis of A. senticosus leaves.This effect is related to the regulation of stomatal regulation. The activity of the photoreaction center is related.

7.
Acta Pharmaceutica Sinica B ; (6): 2339-2347, 2020.
Article in English | WPRIM | ID: wpr-881115

ABSTRACT

Optimization efforts were devoted to discover novel PDE10A inhibitors in order to improve solubility and pharmacokinetics properties for a long-term therapy against pulmonary arterial hypertension (PAH) starting from the previously synthesized inhibitor

8.
Article in Chinese | WPRIM | ID: wpr-847440

ABSTRACT

BACKGROUND: Our preliminary studies have established a rabbit model of knee osteoarthritis using gypsum wire fixation, but the model of traditional Chinese medicine (TCM) syndrome of knee osteoarthritis has not been prepared. OBJECTIVE: To assess the feasibility of establishing a rabbit model of yang deficiency and cold coagulation using peppermint oil and climate chamber, to lay the foundation for future research. METHODS: Thirty-two experimental animals were randomly divided into normal group, model group, yang deficiency group and yang deficiency-cold coagulation group. Except for the normal group, the rabbit knee joints were fixed with plaster fixator brake method for 8 weeks. The yang deficiency group was given daily perfusion of peppermint oil for 2 weeks at the beginning of the 7th week of fixation. In the yang deficiency-cold coagulation group, the rabbits were placed in a climate chamber at 4 °C for 1.5 hours every day, and given daily perfusion of peppermint oil after 6 weeks of braking. After 7 and 14 days of intragastric administration, the serum level of thyrotropin (T4) was detected by ELISA. After 8 weeks of modeling, the articular cartilage, meniscus and synovium were taken for histological scoring. Animal behaviors were observed, and body surface temperature was detected using an infrared thermal imager. The experimental protocol was approved by the Animal Ethics Committee of the First Affiliated Hospital of Xinjiang Medical University (approval No. IACUC-20170222026). RESULTS AND CONCLUSION: For behavior observation, the symptoms of yang deficiency-cold coagulation appeared in the yang deficiency-cold coagulation group. The plantar temperature of the yang deficiency-cold coagulation was lower than that of the normal and model groups (P < 0.05). The serum T4 level was lower in the yang deficiency group and yang deficiency-cold coagulation group than the normal group (P < 0.05). The Mankin scores of the knee joints in two yang deficiency groups were both higher than that in the normal group (P < 0.05) but lower than that in the model group (P < 0.05). Hematoxylin-eosin staining of the meniscus and synovial membrane indicated the yang deficiency group and the yang deficiency-cold coagulation group had no significant difference from the model group. Histomorphological findings elucidated that the peppermint oil with climate box contributes to simulating the disease state to a certain extent, which can provide a reference for establishing the rabbit knee osteoarthritis model of yang deficiency-cold coagulation type.

9.
Article in Chinese | WPRIM | ID: wpr-843174

ABSTRACT

Objective • To investigate the interaction among stress-related negative feelings, depressive symptoms and shape concern of the patients with anorexia nervosa (AN), and the mediating effect of depressive symptoms between negative feelings and shape concern. Methods • A total of 110 AN patients who met the diagnostic criteria of the Diagnosis and Statistical Manual of Mental Disorders (Fifth Edition) (DSM-5) were recruited and their stress-related negative feelings were measured with Perceived Stress Scale (PSS), depressive symptoms were measured with Beck Depression Inventory (BDI), and shape concern was measured with Eating Disorder Examination Questionnaire 6.0 (EDE-Q 6.0). Pearson's correlation test was used to examine the relation between the variables, and the Bootstrap method was used to test the mediating effect of depressive symptoms between negative feelings and shape concern. Results • Negative feelings of the AN patients were positively correlated with depressive symptoms and shape concern (r=0.537, r=0.729, both P=0.000), and depressive symptoms were positively correlated with shape concern (r=0.614, P=0.000). The mediating effect analysis showed that the mediating role of depressive symptoms between negative feelings and shape concern was 0.332 (95%CI 0.156-0.535), with the mediating effect ratio of 61.673%. Conclusion • The depressive symptoms of the AN patients may play a mediating effect between negative feelings and shape concern, and the mediating effect is greater than the direct effect.

10.
International Eye Science ; (12): 2003-2007, 2020.
Article in Chinese | WPRIM | ID: wpr-829255

ABSTRACT

@#The pneumonia caused by Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)abbreviated as Coronavirus Disease 2019(COVID-19)has been classified as a Class B infectious disease under the management of Class A infectious diseases in China. COVID-19 caused by hospital-related transmission is not uncommon and poses a serious threat to clinical medical staff and inpatients. This article introduces that ophthalmologists should master the epidemiological characteristics and clinical manifestations of COVID-19, how ophthalmologists cope with the emergency patients in ophthalmology, focus on the key points of infection prevention and control in ophthalmology emergency and surgery, and strengthen specific prevention and control measures, so as to provide reference for infection prevention and control in ophthalmology medical care in the front line of war epidemics.

11.
Article in Chinese | WPRIM | ID: wpr-781848

ABSTRACT

Biological neural networks have dual properties of small-world attributes and scale-free attributes. Most of the current researches on neural networks are based on small-world networks or scale-free networks with lower clustering coefficient, however, the real brain network is a scale-free network with small-world attributes. In this paper, a scale-free spiking neural network with high clustering coefficient and small-world attribute was constructed. The dynamic evolution process was analyzed from three aspects: synaptic regulation process, firing characteristics and complex network characteristics. The experimental results show that, as time goes by, the synaptic strength gradually decreases and tends to be stable. As a result, the connection strength of the network decreases and tends to be stable; the firing rate of neurons gradually decreases and tends to be stable, and the synchronization becomes worse; the local information transmission efficiency is stable, the global information transmission efficiency is reduced and tends to be stable, and the small-world attributes are relatively stable. The dynamic characteristics vary with time and interact with each other. The regulation of synapses is based on the firing time of neurons, and the regulation of synapses will affect the firing of neurons and complex characteristics of networks. In this paper, a scale-free spiking neural network was constructed, which has biological authenticity. It lays a foundation for the research of artificial neural network and its engineering application.


Subject(s)
Action Potentials , Models, Neurological , Neural Networks, Computer , Neuronal Plasticity , Synapses
12.
Article in Chinese | WPRIM | ID: wpr-774062

ABSTRACT

OBJECTIVE@#To study the significance of dishevelled (DVL) proteins in the Wnt signaling pathway in the pathogenesis and prognosis of childhood acute lymphoblastic leukemia (ALL).@*METHODS@#A total of 33 children with new-onset ALL were enrolled as the case group. According to the degree of risk, they were divided into 3 groups: low-risk (n=14), intermediate-risk (n=5) and high-risk (n=14). A total of 29 children with immune thrombocytopenia were enrolled as the control group. At diagnosis and on day 33 of induction therapy, 2 mL bone marrow samples were collected from the case and control groups, and qRT-PCR was used to measure the mRNA expression of DVL1, DVL2 and DVL3 in blood cells of bone marrow.@*RESULTS@#The mRNA expression of DVL1, DVL2 and DVL3 in the case group in the incipient stage was significantly higher than that in the remission stage and the control group (P<0.05). Compared with the control group, the case group had a significant increase in the mRNA expression of DVL2 in the remission stage (P<0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in both remission and incipient stages (P<0.05). The high- and intermediate-risk groups had significantly higher mRNA expression of DVL1 and DVL2 than the low-risk group (P<0.05). The mRNA expression of DVL2 was significantly higher than that of DVL1 and DVL3 in the low-, intermediate- and high-risk groups (P<0.05).@*CONCLUSIONS@#The change in the expression of DVL, especially DVL2, in the Wnt signal pathway has certain significance in the pathogenesis and prognosis of childhood ALL.


Subject(s)
Child , Dishevelled Proteins , Humans , Phosphoproteins , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Wnt Signaling Pathway
13.
Article in Chinese | WPRIM | ID: wpr-772535

ABSTRACT

OBJECTIVE@#Medical image segmentation is a key step in medical image processing. An architecture of fully convolutional networks was proposed to realize automatic segmentation of anatomical areas in X-ray images.@*METHODS@#Enlightened by the advantages of convolutional neural networks on features extraction, fully convolutional networks consisting of 9 layers were designed to segment medical images. The networks used convolution kernels of various sizes to extract multi-dimensional image features in the images, meanwhile, eliminated pooling layers to avoid the loss of image details during downsampling procedures.@*RESULTS@#The experiment was conducted in accordance with the specific scene of X-ray images segmentation. Compared with traditional segmentation methods, this approach achieved more accurate segmentation of anatomical areas.@*CONCLUSIONS@#Fully convolutional networks can extract representative and multidimensional features of medical images, avoid the loss of image details during downsampling procedures, and complete automatic segmentation of anatomical areas accurately in X-ray images.


Subject(s)
Algorithms , Image Processing, Computer-Assisted , Neural Networks, Computer , X-Rays
14.
Article in Chinese | WPRIM | ID: wpr-775925

ABSTRACT

To further investigate the application of positron emission tomography (PET) technology in acupuncture research field, with "PET" and "acupuncture" as keywords, the related literature published from 1997 to 2018 was searched in PubMed, CNKI and WANFANG database; then the literature was classified and analyzed. The results showed that in clinical and experimental studies, whether in physiological or pathological conditions, PET technology has verified the specificity of acupoints, bidirectional regulation of acupoints, and the clinical effect of -arrival from the level of brain functional activity. It has deeply revealed the central mechanism underlying that acupuncture has multi-target, multi-channel and multi-level overall effects. The purpose of this study is to provide objective scientific basis for acupuncture research, and then potentially guide the clinical practice.


Subject(s)
Acupuncture Points , Acupuncture Therapy , Positron-Emission Tomography
15.
Neuroscience Bulletin ; (6): 205-215, 2019.
Article in English | WPRIM | ID: wpr-775425

ABSTRACT

The locus coeruleus (LC) has been studied in major depressive disorder (MDD) and bipolar disorder (BD). A major problem of immunocytochemical studies in the human LC is interference with the staining of the immunocytochemical end-product by the omnipresent natural brown pigment neuromelanin. Here, we used a multispectral method to untangle the two colors: blue immunocytochemical staining and brown neuromelanin. We found significantly increased tyrosine hydroxylase (TH) in the LC of MDD patients-thus validating the method-but not in BD patients, and we did not find significant changes in the receptor tyrosine-protein kinase ErbB4 in the LC in MDD or BD patients. We observed clear co-localization of ErbB4, TH, and neuromelanin in the LC neurons. The different stress-related molecular changes in the LC may contribute to the different clinical symptoms in MDD and BD.


Subject(s)
Aged , Aged, 80 and over , Bipolar Disorder , Metabolism , Pathology , Depressive Disorder, Major , Metabolism , Pathology , Female , Humans , Image Processing, Computer-Assisted , Immunohistochemistry , Methods , Locus Coeruleus , Metabolism , Pathology , Male , Melanins , Metabolism , Microscopy , Methods , Middle Aged , Neurons , Metabolism , Pathology , Receptor, ErbB-4 , Metabolism , Sensitivity and Specificity , Spectrum Analysis , Methods , Tyrosine 3-Monooxygenase , Metabolism
16.
Chinese Medical Journal ; (24): 680-689, 2019.
Article in English | WPRIM | ID: wpr-774770

ABSTRACT

BACKGROUND@#Several patients experience persistent otorrhea after a flawless surgical procedure because of insufficient epithelial healing. Several efforts, such as autologous tissue allograft and xenograft, have been made to halt otorrhea. However, a stable technology to induce temporal epithelial repair is yet to be established. Therefore, this study aims to investigate whether implantation of seeding adipose-derived mesenchymal stem cell (ADMSC) aggregates on extracellular matrix (ECM; herein, ADMSC aggregate-ECM) into damaged skin wound promotes skin regeneration.@*METHODS@#ADMSC aggregate-ECM was prepared using a previously described procedure that isolated ADMSCs from rabbits and applied to the auricle and auditory meatus wound beds of New Zealand white rabbits. Wound healing was assessed by general observation and hematoxylin and eosin (H&E) staining. Secretion of growth factor of the tissue was evaluated by western blotting. Two other groups, namely, ECM and control, were used. Comparisons of three groups were conducted by one-way analysis of variance analysis.@*RESULTS@#ADMSCs adhered tightly to the ECM and quickly formed cell sheets. At 2 weeks, general observation and H&E staining indicated that the wound healing rates in the ADMSC aggregate-ECM (69.02 ± 6.36%) and ECM (59.32 ± 4.10%) groups were higher than that in the control group (43.74 ± 12.15%; P = 0.005, P < 0.001, respectively) in ear auricle excisional wounds. At 7 weeks, The scar elevation index was evidently reduced in the ADMSC aggregate-ECM (2.08 ± 0.87) and ECM (2.31 ± 0.33) groups compared with the control group (4.06 ± 0.45; P < 0.001, P < 0.001, respectively). In addition, the scar elevation index of the ADMSC aggregate-ECM group reached the lowest rate 4 weeks in advance. In auditory meatus excisional wounds, the ADMSC aggregate-ECM group had the largest range of normal skin-like structure at 4 weeks. The ADMSC aggregate-ECM and ECM groups secreted increased amounts of growth factors that contributed to skin regeneration at weeks 1 and 2, respectively.@*CONCLUSIONS@#ADMSC aggregate-ECM and ECM are effective repair materials for wound healing, especially ADMSC aggregate-ECM. This approach will provide a meaningful experimental basis for mastoid epithelium repair in subsequent clinical trials.


Subject(s)
Adipose Tissue , Cell Biology , Animals , Cell Differentiation , Physiology , Cell Proliferation , Physiology , Cells, Cultured , Ear Auricle , Cell Biology , Extracellular Matrix , Chemistry , Flow Cytometry , Mesenchymal Stem Cell Transplantation , Methods , Mesenchymal Stem Cells , Cell Biology , Microscopy, Electron, Scanning , Osteogenesis , Physiology , Rabbits , Real-Time Polymerase Chain Reaction
17.
Article in Chinese | WPRIM | ID: wpr-774527

ABSTRACT

The present study was conducted to explore the effect of endophytic fungi fraction on growth and anti-oxidative activity of Eleutherococcus senticosus. The growth,yield,contents of MDA,and antioxidant activities were assessed in E. senticosus under five fungi fractions,namely BZ,MH,DT,JS,and XFZ. The results showed that fungi fractions and component significantly affected the growth,low concentration of DT fungi fraction significantly increased the biomass of E. senticosus,reduced the MDA content in cells,and the antioxidant activities of the aqueous extracts were superior to the others. The results indicated that low concentration of DT fungi fraction was the optimum fraction to achieve high yield and quality of E. senticosus.


Subject(s)
Antioxidants , Metabolism , Eleutherococcus , Metabolism , Fungi , Chemistry , Malondialdehyde , Metabolism , Oxidative Stress
18.
Article in Chinese | WPRIM | ID: wpr-802306

ABSTRACT

Objective:To discuss the effect of volatile oil from Xanthii Fructus on airway remodeling of rats with bronchial asthma, in order to discuss the mechanism of action through matrix metalloteinases-9 (MMP-9), tissue inhibitor of metalloproteinase-1 (TIMP-1), platelet derived growth factor (PDGF), transforming growth factor-β1 (TGF-β1), Smad2, Smad3 and Smad7 mRNA expressions. Method:Forty-eight rats (half males and half females) were randomly divided into six groups (n=8), namely normal saline group, model group, volatile oil from Xanthii Fructus (7.5, 15, 30 mg·kg-1) groups and dexamethasone group (0.5 mg·kg-1). Except for normal saline group, airway remodeling of rats were established through ovalbumin sensitization and atomization inhalation method. At volatile oil group, rats got volatile oil spray since the first time (the 4th week after modeling) of inspiration of asthma to the last day before the end of test. Histopathological changes of bronchus and lung were analyzed by htoxylin eosin (HE) stain. And airway remodeling was observed, and perimeter of bronchial lumen (Pi), smooth muscle area of bronchi (S), tube wall area (W) and nuclei of smooth muscle cells (N) were recorded. And levels of endothelin-1 (ET-1) and insulin-like growth factor-1(IGF-1) in irrigation solution of Alveoli (BALF) were detected. And mRNA expressions of MMP-9, TIMP-1, PDGF, TGF-β1, Smad2, Smad3 and Smad7 were detected by real-time quantitative-polymerase chain reaction (Real-time PCR). Result:In the model group, infiltration of a large number of inflammatory cells were found at the peribronchial wall, airway smooth muscle thickness and basement membrane increased, and bureaucratic cavity was narrower and flatter. Volatile oil from Xanthii Fructus can ameliorate pathological damage of bronchopulmonary tissue. Levels of S/Pi, W/Pi and N/Pi, BALF, ET-1, IGF-1 and MMP-9/TIMP-1 and mRNA expressions of MMP-9 and PDGF in volatile oil group and dexamethasone group were lower than those in model group (Pβ1, Smad2 and Smad3 were less than those in model group (PPConclusion:Volatile oil from Xanthii Fructus can regulate levels of MMP-9, TIMP-1, MMP-9/TIMP-1, TGF-β1/Smad, inhibit expressions of PDGF, ET-1 and IGF-1, and suppress subepithelial fibrosis, extracellular matrix synthesis, proliferation and migration of airway smooth muscle cells to relieve or inhibit airway remodeling, so as to prevent and treat asthma.

19.
Article in Chinese | WPRIM | ID: wpr-756214

ABSTRACT

Objective To analyze how enterovirus D68 (EV-D68) protease 2A affects the anti-vi-ral interferon typeⅠ(IFN-Ⅰ) pathway in 293T cells following infection. Methods Western blot was used to detect the expression of recombinant protease 2A, IFN-α and signal transducers and activators of tran-scription 1 (STAT1) at protein level. Expression of EV-D68 viral protein (VP1) and protease 2A was ana-lyzed by immunofluorescence at different time points. Cytopathic effects were recorded to calculate 50% cell culture infective dose ( CCID50 ) . Expression of the genes involved in the anti-viral IFN-Ⅰ pathway was measured by real-time PCR (RT-PCR). Results The recombinant plasmid pCLIPf-2A was successfully constructed and the expression of recombinant protease 2A could be detected by Western blot 24 h after transfection. The recombinant protease 2A promoted the proliferation of EV-D68 at the late stage of infection and induced the production of IFN-α. Expression of the genes involved in the anti-viral IFN-Ⅰ pathway at mRNA level was up- or down-regulated to different degrees with various trends in different groups following infection. Expression of STAT1 was enhanced in all groups. Conclusions EV-D68 protease 2A promoted the activation of anti-viral IFN-Ⅰpathway in response to viral infection and enhanced the proliferation of virus at the late stage of infection.

20.
Article in Chinese | WPRIM | ID: wpr-755638

ABSTRACT

Objective To evaluate the effect of labor analgesia on maternal and neonatal outcomes. Methods The medical records of mature puerperas with singleton pregnancy from October 2018 to April 2019 at the General Hospital of Ningxia Medical University were retrospectively reviewed. Puerperas were divided into non-labor analgesia group ( NLA) and labor analgesia group ( LA) according to whether or not puerperas received labor analgesia. Maternal and neonatal outcomes were analyzed. Maternal outcomes in-cluded delivery mode, complications, intrapartum hemorrhage and blood loss at 24 h after birth, duration of the second stage of labor and prolongation, episiotomy and length of hospital stay after birth. Neonatal outcomes included Apgar score at 1, 5 and 10 min after birth, cases of Apgar score<7 at 5 min after birth, and the proportion and reason of transfer to pediatrics unit. Results A total of 839 puerperas were includ-ed, with 551 cases in NLA group and 288 cases in LA group. Compared with NLA group, the second stage of labor was significantly prolonged ( P<0. 01) , and no significant change was found in the other maternal or neonatal outcomes in LA group ( P>0. 05) . Conclusion Labor analgesia can prolong the second stage of labor and exerts no effect on the neonatal outcomes.

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