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1.
Journal of Preventive Medicine ; (12): 277-281, 2022.
Article in Chinese | WPRIM | ID: wpr-920767

ABSTRACT

Objective@#To investigate immune responses to influenza virus infections and the immunogenicity of trivalent split-virus influenza vaccine among elderly populations in Jiangbei District, Ningbo City, so as to provide the support for promoting influenza vaccination among elderly populations.@*Methods@#The elderly populations at ages of 60 years and older were recruited in Jiangbei District of Ningbo City from September to November, 2020, and the participants were assigned to the vaccination group and the control group according to vaccination intention. The titers of haemagglutination inhibition ( HI ) antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were measured using the micro HI test prior to vaccination and 30 days post-vaccination, and the protective rate, geometric mean titer ( GMT ) and seroconversion rate of antibodies were analyzed before and after vaccination.@*Results@#There were 290 participants in the vaccination group, including 132 men (45.52% ), and 290 controls, including 132 men ( 45.52% ). There were no significant differences between the vaccination group and the control group in terms of the protective rate or GMT of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV prior to vaccination ( P>0.05 ). Following vaccination, the protective rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were 98.62%, 94.14% and 88.28%, and the GMT of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV increased by 9.26, 6.19 and 10.09 folds, while the seroconversion rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were 78.62%, 68.28% and 71.38%, respectively. The protective rates, GMT and seroconversion rates of antibodies against influenza viruses A ( H1N1 and H3N2 ) and BV were all significantly greater in the vaccination group than in the control group post-vaccination ( P<0.05 ). A lower increase was seen in the GMT of antibodies against the influenza virus BV among residents at ages of 80 years and older (increase by 7.91 folds) than among residents at ages of 70 to 79 years ( increase by 12.53 folds ) and 60 to 69 years (increase by 13.32 folds) in the vaccination group post-vaccination ( P<0.05 ), and the seroconversion rate of antibodies against the influenza virus BV was significantly lower in residents at ages of 80 years and older ( 62.57% ) than in those at ages of 70 to 79 years ( 83.33% ) ( P<0.05 ), while the positive conversion rate of antibodies against the influenza virus A ( H3N2 ) was significantly lower in residents at ages of 80 years and older ( 62.57% ) than in those at ages of 60 to 69 years ( 91.30% ) ( P<0.05 ).@*Conclusions@#Low-level immune responses are detected to antibodies against influenza virus A ( H3N2 ) and BV among elderly populations in Jiangbei District of Ningbo City, and trivalent split-virus influenza vaccine shows a high immunogenicity among elder populations. An emphases on improvements in coverage of influenza vaccination among elderly populations at ages of 60 to 69 years, and development of influenza vaccines with a higher protective efficacy for residents at ages of 80 years and older are recommended.

2.
Article in Chinese | WPRIM | ID: wpr-906222

ABSTRACT

Objective:To compare the efficacy and safety of 10 kinds of oral Chinese patent medicines combined with chemotherapy in the treatment of gastric cancer in a network Meta-analysis. Method:PubMed,Embase,Web of Science,Cochrane Library,CNKI,China biomedical literature service system,VIP,Wanfang database were retrieved to obtain Chinese and English literatures published before March 1,2020. The randomized controlled trials(RCTs) and data were screened and extracted. The quality of the included literatures was also evaluated. The pair-work Meta-analysis was conducted by Revman 5.3 software, and the Meta-analysis were conducted by ADDIS 1.16.7 software. Result:A total of 41 randomized controlled trials were eventually involved. All of them were two-arm,including 10 oral Chinese patent medicines,Cinobufotalin,Jinlong capsule,Pingxiao capsule,Shenyi capsule,Xihuang pill (or capsule),Xiaoaiping tablet,Yangzheng Xiaoji capsule,Zhenqi Fuzheng granule combined with chemotherapy for gastric cancer. The effective rate for treating cancer was significantly higher than that of chemotherapy alone (<italic>P</italic><0.05). Zhenqi Fuzheng+chemotherapy was the best choice; the KPS improvement rate of Cinobufacin,Shenyi capsule,Yangzheng Xiaoji capsule,Zhenqi Fuzheng granule combined with chemotherapy in the treatment of gastric cancer was significantly increased(<italic>P</italic><0.05). Shenyi capsule combined with chemotherapy might be the best regimen. The incidence rate of leukocyte decline was significantly lower in the treatment of gastric cancer(<italic>P</italic><0.05). The combination of Zhenqi Fuzheng granule and chemotherapy was the best choice. Traditional Chinese medicine could not reduce the incidence of neurotoxicity(<italic>P></italic>0.05). Cinobufufacin,Yangzheng Xiaoji capsule and Zhenqi Fuzheng granule combined with chemotherapy could increase CD4<sup>+</sup>/CD8<sup>+ </sup>in patients with gastric cancer, which was significantly higher than that of chemotherapy alone(<italic>P</italic><0.05). Conclusion:All kinds of oral Chinese patent medicines can play a significantly role in the treatment of gastric cancer. Zhenqi Fuzheng granule,angzheng Xiaoji capsule and Shenyi capsule combined with chemotherapy are more effective in treating gastric cancer.

3.
Article in Chinese | WPRIM | ID: wpr-912793

ABSTRACT

The electronic nursing clinical pathway was fully applied, and the evidence-based practice achievements of cancer patient symptom management was integrated into the electronic nursing clinical pathway in Fudan University Shanghai Cancer Center. Taking " comprehensive evaluation before chemotherapy" , " chemotherapy-related nausea and vomiting" , " chemotherapy-related diarrhea" as examples, the authors introduced the application of evidence-based practice project in nursing clinical pathway. Through the implementation of the project, a standardized operation flow of electronic nursing clinical pathway was formed; The nurses introduced new nursing tools, new processes and new technologies in the process of project implementation; Meanwhile, the project reduced the incidence of adverse symptoms and shortened the hospitalization time of patients. The project achieved the goal of " win-win" to reduce the burden of patients′ disease and improve the efficiency of tumor care.

4.
Chinese Journal of Rheumatology ; (12): 584-589, 2021.
Article in Chinese | WPRIM | ID: wpr-910205

ABSTRACT

Objective:To explore the clinical application and long-term safety of hydroxychloroquine sulfate (HCQ) in the treatment of rheumatic diseases.Methods:A multi-center cross-sectional study was conducted between August 2017 and August 2018 in a random sample of eleven medical institutions of rheumatology and immunology in China. Patients who took HCQ for more than 3 months were enrolled into this study. The cumulative dose and long-term side effects of HCQ were recorded. The changes of laboratory indexes before and after treatment with HCQ were analyzed. Categorical variables were presented with counts and proportions, and evaluated by Chi-square test. Continuous parametric data were presented as Mean±standard deviation, and evaluated by Student's t test or Mann-Whitney U test. P-values less than 0.05 were considered statistically significant. Results:A total of 886 patients with rheumatic diseases were enrolled into this study, including 505 cases with systemic lupus erythematosus (57.0%), 210 cases with rheumatoid arthritis (23.7%), 80 cases with Sj?gren's syndrome (9.0%), 57 cases with undifferentiated connective tissue disease (6.4%), 12 cases of systemic vasculitis (1.4%), 10 cases of mixed connective tissue disease (1.1%), 7 cases of myositis (0.8%) and 5 cases with systemic sclerosis (0.6%). The most common long-term side effects of HCQ was skin or mucous lesions (12.4%) and vision problems (8.0%). Other adverse reactions included problems of digestive system (3.0%), nervous system (2.1%), musculoskeletal system (1.1%) and cardiovascular system (0.9%). 140 cases (15.8%) had stopped taking HCQ during the treatment. More than half of them decided to stop taking medicine by themselves. Fifty-four patients (6.1%) stopped using HCQ due to side effects while 24 of them took it again, and another 12 patients (1.4%) stopped the drug due to remission of illness. Patients were divided into three groups according to the cumulative dose of HCQ: less than 500 g, 500-1 000 g and more than 1 000 g respectively. There was significant difference in the incidence of long-term side effects among the three groups ( χ2=6.382, P=0.041). The last group (more than 1 000 g) suffered the highest incidence of long-term adverse reactions (37.1%). No severe adverse drug reactions were observed in this study. Conclusion:Hydroxychloroquine is widely used in the treatment of rheumatic diseases. The incidence of long-term side effects is 20.4%, is 6.1% lead to drug withdrawal, which are especially related to the cumulative doses. It should be adjusted properly according to the clinical application.

5.
Journal of Chinese Physician ; (12): 1158-1163, 2021.
Article in Chinese | WPRIM | ID: wpr-909679

ABSTRACT

Objective:To analyze the factors affecting the disappearance time of airway necrosis and repair time of airway scar stenosis in patients with ulceration necrosis tracheobronchial tuberculosis (TBTB Ⅱ) after standardized chemotherapy and bronchoscopic intervention.Methods:The clinical data of 222 TBTB Ⅱ patients admitted to Hunan Chest Hospital from January 2015 to December 2018 were collected, bronchoscopic interventional treatment was performed on time. The texture, blockage of lumen, granulation proliferation, airway stenosis of TBTB patients before treatment, the disappearance time of airway dead objects, scar repair time and stenosis degree after treatment were followed up. The disappearance time of airway necrosis and repair time of airway scar stenosis and its influencing factors were recorded and analyzed.Results:In 222 patients, 508 ulceration necrosis airway lesions were found under bronchoscopy, with a median of 2(1-6); 170(76.6%) cases of airway lesions had different degrees of stenosis before treatment. 79(35.6%) patients had tough necrosis, and 86(38.7%) patients had necrosis blocking the lumen; 132(59.5%) patients had granulomatosis. The disappearance time of airway necrosis after treatment was 1 to 32 weeks, and M( Q1, Q3) was 6(3, 9) weeks; the repair time of airway scar stenosis was 2 to 73 weeks, and M( Q1, Q3) was 14(10, 19) weeks; after treatment, there were 90.5%(201/222) patients with different degrees of scarring in the airways. Cox multiple analysis showed that the risk factor for the disappearance time of airway necrosis was tough tough necrosis ( HR=1.52, 95% CI: 1.10-2.10); the risk factor for the repair time of airway scar stenosis was the disappearance time of airway necrosis 6-9 weeks ( HR=2.73, 95% CI: 1.84-4.05). Conclusions:90.5% of patients with type Ⅱ TBTB developed airway scar stenosis after treatment. The median time for the disappearance of airway necrosis was 6 weeks, and the median time for the repair time of airway scar stenosis was 14 weeks. In the interventional process, attention should be paid to the removal of tough necrosis and the efficiency of necrosis removal to reduce the risk of airway scar stenosis.

6.
Article in Chinese | WPRIM | ID: wpr-909579

ABSTRACT

OBJECTIVE Only limited number of drugs are currently available for treating ischemic stroke. Therapeu?tic angiogenesis has recently emerged as one of the most promising therapies for cerebral ischemic injury. Isopropyl-β-(3,4-dihydroxyphenyl)-α-hydroxypropanoate (IDHP) is a metabolite derived from the botanical formulation for Dantonic?. Here, we investigated the angiogenic efficacy of IDHP in cerebral ischemia. METHODS The in vivo effects of IDHP were evaluated in the C57BL/6 mouse Matrigel plug and rat transient middle cerebral artery occlusion (tMCAO) models. Primary human umbilical vein endothelial cells (HUVEC) and human brain microvascular endothelial cells (HBMEC) were used to explore the effects of IDHP on stimulating proliferation, migration and tube formation in vitro. ELISA and Western blotting were used to quantitate the release and expression of relevant target molecules and signaling path?ways. RESULTS IDHP reduced infarct volume and improved sensorimotor function in rats subjected to tMCAO by pro?moting angiogenesis, and promoted Matrigel neovascularization in mice. Moreover, IDHP produced a biphasic modula?tion on proliferation and migration both in HUVEC and HBMEC. It also induced tube formation in a 12-day HUVEC-HDF co-culture model and in Matrigel assays. IDHP-induced angiogenesis was accompanied by increased levels of p-AMPKα (Thr172) and p-eNOS (Ser1177) both in vitro and in vivo, and the decreased level of VEGF in rat brains on day 1 whereas enhanced level of VEGF on day 3 and 7 after tMCAO. Mechanistically, AMPK knockdown or pharmacologi?cally inhibiting AMPK and its upstream kinases (CaMKKβ) inhibited the eNOS phosphorylation induced by IDHP in HUVEC. Furthermore, selective eNOS inhibitor (L-NIO), selective CaMKKβ inhibitor (STO) and AMPKa inhibitor (Com?pound C) blocked the capillary-like tube formation in the co-culture model induced by IDHP (10 nmol · L-1). CONCLU?SION Collectively, these findings showed that IDHP protected rats from cerebral ischemia-reperfusion injury by promot?ing angiogenesis via activating CaMKKβ/AMPK(Thr172)/eNOS(Ser1177) signaling, and suggest it to be a promising new drug candidate for the prevention and/or treatment of cerebral ischemia and other vascular occlusive diseases.

7.
Article in Chinese | WPRIM | ID: wpr-885127

ABSTRACT

Objective:To examine the correlations of microRNA-34c(miR-34c) expression in the peripheral blood with the onset of diabetic foot ulcer(DFU)and diabetic foot osteomyelitis(DFO)in patients with type 2 diabetes mellitus(T2DM).Methods:Sixty newly-diagnosed T2DM patients without DFU(T2DM group), 112 T2DM patients with DFU(DFU group), and 60 healthy controls with normal glucose tolerance(NC group)were included. The 112 T2DM patients with DFU were further divided into DFO( n=64)and NDFO( n=48)groups. The levels of miR-34c were determined by quantitative real-time PCR, while clinical features and risk factors of DFU and DFO were explored. Results:A significant increase in the expression level of miR-34c in peripheral blood was observed in T2DM group compared with NC group[2.99(1.45-6.22) vs 1.01(0.89-1.52), P<0.05], and a markedly increased miR-34c expression level was noted in DFU group compared with T2DM group [9.65(6.15-18.63) vs 2.99(1.45-6.22), P<0.01]. Additionally, the expression level of miR-34c in peripheral blood significantly increased in DFO group compared to NDFO group [13.46(8.89-19.11) vs 6.02(5.93-14.72), P<0.01]. Moreover, there was a positive correlation between the expression level of miR-34c in peripheral blood and the amputation rate in patients in DFU group( P=0.030), and a negative correlation in the expression level of miR-34c( P=0.025)with healing rate of DFU after eight weeks. The multivariate logistic regression analysis confirmed that a high expression of miR-34c was an independent risk factor for DFU and DFO( OR=3.52, 4.13; both P<0.01). Conclusion:An increased expression of miR-34c in peripheral blood of T2DM patients might be closely related to the occurrence, development, and prognosis of DFU and DFO.

8.
Chinese Journal of Rheumatology ; (12): 171-175,F3, 2021.
Article in Chinese | WPRIM | ID: wpr-884385

ABSTRACT

Objective:To observe the expression of interleukin (IL)-38 in patients with active systemic lupus erythematosus (SLE), and to explore the regulatory effect of IL-38 on IκB kinase complex.Methods:The gene expression levels of IL-38 and IκB kinase complex were detected by real-time fluorescence quantitative polymerase chain reaction (PCR). Serum IL-38 levels were detected by enzyme-linked immunosorbent assay (ELISA). The expression of IκB-kinase complex (IκK)α/β and phospho IκKα/β protein weredetermined by Western blotting analysis. Statistical analysis was conducted with or Mann-Whitney rank test.Results:① The expression level of IL-38 mRNA in active SLE patients (0.36±0.09) was significantly lower than that in the normal control group (1.00±0.17) ( Z=-4.07, P<0.01); the expression level of IL-38 protein in active SLE patients (5.86±2.76) significantly reduced as compared with normal control group (18.48±1.35) ( Z=-4.76, P<0.05). ② The expression level of IκKα mRNA (7.45±0.31) and IκKβ mRNA (6.01±1.51) in active SLE patients was significantly higher than that in normal control group (1.16±0.04) and (1.16±0.14) ( Z=-4.67, P<0.05; Z=-4.37, P<0.01), and the expression level of IL-38 mRNA was negatively correlated with IκKα mRNA and IκKβ mRNA in active SLE patients ( r=-0.78, P<0.05; r=-0.83, P<0.05). ③ IκKα/β and phosphorylated IκKα/β protein expression in active SLE patients (2.38±0.03) and (1.90±0.03) increased significantly compared with healthy controls (1.00±0.04) and (1.00±0.08) ( Z=-1.96, P<0.05; Z=-1.99, P<0.05). ④ In vitro experiment, IL-38 caused a significant decrease in the expression level of IκKα mRNA (1.70±0.12) and IκKβ mRNA (2.52±0.10) from active SLE patientscompared with untreated cells (2.56±0.28) and (3.82±0.38) ( Z=-1.96, P<0.05; Z=-1.37, P<0.05). ⑤ In vitro experiment, IL-38 caused a significant decrease in the expression level of IκKα/β (1.54±0.06) and phosphoryl-ated IκKα/β (0.970±0.012) protein expression in active SLE patients compared with untreated cells (2.93±0.08) and (1.572±0.051)( P<0.05). Conclusion:The de-crease of IL-38 level in SLE patients result in the excessive activation of IκB kinase complex IκKα and IκKβ, and therefore triggersystemic lupus erythematosus.

9.
Article in Chinese | WPRIM | ID: wpr-883970

ABSTRACT

Objective:To study the effect of social support on depression-posttraumatic stress disorder(PTSD) comorbidity among people living with human immunodeficiency virus/acquired immunodeficiency syndrome(PLWHA).Methods:Using questionnaire to one-to-one investigate the newly-diagnosed PLWHA in Wuhan Jingyintan Hospital HIV/AIDS clinic from October 2016 to February 2019. The content of the questionnaire included general demographic characteristics, HIV-related high-risk behaviors before diagnosis, social support, depression and PTSD.Patients with depression and PTSD were collected as P+ D group.Patients without depression or PTSD were collected as N group. The statistical software was SPSS 21.0. Chi-square test, t test and rank sum test were used to compare the differences between the two groups, and multivariate Logistic regression analysis was used to identify the influencing factors of depression combined with PTSD. Results:Among 320 PLWHA, 72 subjects(22.50%) had depression with PTSD(P+ D group), 161 subjects(50.31%) had neither depression nor PTSD(N group). Between the two groups, the differences of constituent ratios of gender(χ 2=9.84), routes of infection(χ 2=11.16), whether ever used drug(χ 2=30.00)were statistically significant(all P<0.05). There were statistically significant differences in the scores of social support((30.64±10.90) vs (25.51±8.55)), objective social support((7.20±3.08) vs (5.76±2.24)), subjective social support((16.70±6.87) vs (14.04±5.61)) and utility of social support((6.74±2.59) vs (5.71±2.60)( t=3.56, 3.57, 2.88, 2.81, all P<0.05). The results of Logistic analysis revealed that gender as male( β=-1.48, OR= 0.23, 95% CI=0.09-0.61) was protective factor for depression co-occuring with PTSD, while lower level of social support ( β=1.40, OR=4.05, 95% CI=1.36-12.10) was a risk factor. Conclusion:The prevalence of depression co-occuring with PTSD in PLWHA is high. The influence of gender and social support should be paid more attention to PLWHA.

10.
Article in Chinese | WPRIM | ID: wpr-882014

ABSTRACT

Objective To determine the effects of the"double random"supervision model of public places in Yangpu District, Shanghai. Methods Using the"double random"data of public places in Yangpu District of Shanghai from 2018 to 2019, we determined the proportion of unqualified public places assessed by daily supervision and"double random"supervision in 2018 and 2019. Moreover, we identified that if the public places that had been penalized in 2018 would be penalized in 2019, which may reflect the effects of the"double random"supervision model. Results The proportion of unqualified public places assessed by"double random"supervision was significantly higher than daily supervision in 2018(χ2 = 58.04, P < 0.05)and 2019(χ2 = 79.24, P < 0.05);however, the proportion of being penalized in the following year between"double random"supervision and daily supervision had no significant difference(χ2 = 1.81, P = 0.18). Conclusion "Double random"supervision may be easier to identify the problems of the supervision subjects than daily supervision; however, it warrants continual efforts to consolidate the regulatory effects.

11.
Article in English | WPRIM | ID: wpr-880666

ABSTRACT

OBJECTIVES@#Due to the genetic feature of high diversity than other DNA markers, short tandem repeat (STR) plays key roles in forensic, anthropology, and population genetics. Newly introduced multiple STR kit is more valuable because of the greatly improved discriminatory power with the increase in the number of STR loci. The genetic polymorphic data are essential for the application and research in specific population. This study aims to investigate the genetic polymorphism of Han population residing in Yuncheng district, Shanxi Province, to evaluate the application of 23 STR loci in forensic personal identification and paternity test, and to explore the genetic relationship of Han population between Yuncheng and other populations.@*METHODS@#A total of 23 STR loci were amplified from 525 healthy unrelated individuals from the Han nationality in Yuncheng, Shanxi Province using the AGCU EX25 amplification kit. The products were detected and separated by ABI 3500 Genetic Analyzer. Alleles were genotyped by GeneMapper ID (Version 3.2) software, and corresponding frequencies and forensic parameters were calculated. We calculated the genetic distance and plotted the neighboring-joining tree with other 13 population.@*RESULTS@#The allele frequency of the 23 STRs ranged from 0.0010 to 0.5090. No deviation from Hardy-Weinberg equilibrium (@*CONCLUSIONS@#These 23 STRs are highly genetic polymorphic and informative in the Han population of Yuncheng, Shanxi Province, which can provide basic data for forensic personal identification, paternity testing, and population genetic research.


Subject(s)
Asian Continental Ancestry Group/genetics , China , Ethnic Groups/genetics , Gene Frequency , Genetic Loci , Genetics, Population , Humans , Microsatellite Repeats/genetics , Polymorphism, Genetic
12.
Article in Chinese | WPRIM | ID: wpr-879055

ABSTRACT

The rat everted intestinal sac model was adopted to investigate the absorption of total flavonoids from Coreopsis tinctoria in different intestinal segments. Cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, iso-okanin, marein and 3,5-dicaffeoylquinic acid which as the major chemical components of total flavonoids from C. tinctoria were selec-ted as the study objects to evaluate the absorption characteristics of each component in different intestinal segments. The results showed that the absorption of seven components of total flavonoids at different intestinal segments was in consistent with zero order absorption rate. The K_a of chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, isookanin and 3,5-dicaffeoylquinic acid increased with increasing of concentration of total flavonoids(P<0.05), indicating that the intestinal absorption of these five components was passive transport. The K_a of cyaniding-3-O-β-D-glucoside and marein showed a weak concentration dependence, suggesting that the absorption of them may be an positive and passive co-existing mode. The result of absorption in different intestinal segments showed that cyaniding-3-O-β-D-glucoside, chlorogenic acid, flavanomarein, quercetagetin-7-O-β-D-glucoside, marein and 3,5-dicaffeoylquinic acid were mainly absorbed in ileum, while isookanin was mainly absorbed in jejunum. The total flavonoids of C. tinctoria are selectively absorbed in intestinal tract, the rat everted intestinal sac model can be used to evaluate the multi-component intestinal absorption characteristics of total flavonoids from C. tinctoria.


Subject(s)
Animals , Chlorogenic Acid , Coreopsis , Flavonoids , Intestinal Absorption , Plant Extracts , Rats
13.
Article in English | WPRIM | ID: wpr-922466

ABSTRACT

Unrestrained inflammation is harmful to tissue repair and regeneration. Immune cell membrane-camouflaged nanoparticles have been proven to show promise as inflammation targets and multitargeted inflammation controls in the treatment of severe inflammation. Prevention and early intervention of inflammation can reduce the risk of irreversible tissue damage and loss of function, but no cell membrane-camouflaged nanotechnology has been reported to achieve stage-specific treatment in these conditions. In this study, we investigated the prophylactic and therapeutic efficacy of fibroblast membrane-camouflaged nanoparticles for topical treatment of early inflammation (early pulpitis as the model) with the help of in-depth bioinformatics and molecular biology investigations in vitro and in vivo. Nanoparticles have been proven to act as sentinels to detect and competitively neutralize invasive Escherichia coli lipopolysaccharide (E. coli LPS) with resident fibroblasts to effectively inhibit the activation of intricate signaling pathways. Moreover, nanoparticles can alleviate the secretion of multiple inflammatory cytokines to achieve multitargeted anti-inflammatory effects, attenuating inflammatory conditions in the early stage. Our work verified the feasibility of fibroblast membrane-camouflaged nanoparticles for inflammation treatment in the early stage, which widens the potential cell types for inflammation regulation.


Subject(s)
Escherichia coli , Fibroblasts , Humans , Inflammation/drug therapy , Nanoparticles
14.
Article in Chinese | WPRIM | ID: wpr-837479

ABSTRACT

Objective To estimate and predict the characteristics and development trend of the AIDS epidemic situation in Wuhan by using Estimation and Projection Package (EPP) and Spectrum model. Methods Data from HIV/AIDS case reports, sentinel surveillance, special investigations, maternal screenings, detention places reports, antiviral treatments, and screenings of high-risk populations was collected, verified and sorted out. Estimation and projection package (EPP) and Spectrum models were used to fit and modify the curves. The final results were formed after consultation and discussion with experts. Results The EPP/Spectrum model predicted that the HIV infection rate of adults in Wuhan increased significantly after 2003, reached the peak around 2013, and then maintained a high level until 2019. The HIV infection rate of adults in 2019 was about 9.0/105, and the adult infection rate would show a downward trend after 2020. Among all kinds of populations, the infection rate of spouses/sexual partners of already infected people was the highest, with the infection rate in men who have sex with men (MSM) ranking second. The number of people living with HIV/AIDS increased gradually from 2000, and by 2022 the number is estimated to be around 8,000 (95% CI:5421-9754). In regards to gender and age, the main distribution of HIV infections was among young adults, especially men aged 25-50 years old. Conclusion Wuhan currently is a low prevalence area of AIDS. MSM will still be the focus of intervention in the following years.

15.
Article in Chinese | WPRIM | ID: wpr-864308

ABSTRACT

Adenovirus (AdV) infection has been prevalent in the world for more than 60 years, with occasional local outbreaks and epidemics.Through gene recombination, new genotypes of AdV have been discovered continuously, showing varying pathogenic characteristics.Genotypes prevalent in different countries or regions are diverse.AdV infection can cause respiratory, gastrointestinal, urinary and eye diseases.The incidence and mortality of AdV respiratory tract infection are high in children, and there are many severe cases.At present, no antiviral drug approved to treat AdV infection.Severe AdV pneumonia in children has a fatality rate exceeding 50%, which becomes a difficult problem in the treatment of severe pneumonia in intensive care unit.This paper reviews the research progress of AdV infection, and summarizes the related research of respiratory system diseases in children with AdV infection, so as to improve the understanding of children with AdV infection related respiratory diseases.

16.
Article in Chinese | WPRIM | ID: wpr-862515

ABSTRACT

Objective To explore the epidemic characteristics and spatial clustering of pulmonary tuberculosis in Wuhan from 2011 to 2019, and to provide a scientific basis for formulating intervention strategies and measures. Methods Descriptive statistical analysis was conducted on the epidemic characteristics of tuberculosis data in Wuhan registered in the national tuberculosis information management system in the last 9 years, and spatial autocorrelation analysis was performed on the incidence of tuberculosis in 155 communities or in the city using Arcgis10.5 software. Results From 2011 to 2019, there were 56,432 cases of tuberculosis reported in Wuhan, and the annual average reported incidence rate of tuberculosis was 59.24/100 000. The overall incidence rate showed a fluctuating downward trend, with an average annual decline rate of 1.99%. The ratio of the number of cases between men and women was 2.35:1, and the incidence rate in males was higher than that in females (χ2=285.36,P0,P<0.001), and the high-high aggregation areas of tuberculosis were mainly distributed in Erqi community, Baibuting community, Liujiaoting community, Yijiadun community, Heping Street, Changqian Street, Tonghu farm, Yuxian Town, Zhifang Town, Wulijie Town, Fenghuang Street, Liji Street, and Daoguanhe Street. Conclusion The overall epidemic situation of pulmonary tuberculosis in Wuhan showed a slow downward trend. The main population and the clustering time of cases were relatively fixed, and the overall epidemic showed a certain spatial clustering. Active screening should be carried out for high-risk populations and high-aggregation areas, and effective prevention and control strategies should be developed based on time and location classification.

17.
Article in English | WPRIM | ID: wpr-880316

ABSTRACT

BACKGROUND@#The aryl hydrocarbon receptor (AhR) is commonly known as an environmental sensor. Polymorphisms in AhR gene have been implicated in susceptibility to cancer. However, the results were controversial. This study was conducted to quantitatively summarize the association between AhR polymorphisms and cancer risk by meta-analysis.@*METHODS@#Relevant reports were searched in four databases (Embase, PubMed, Wanfang, and China National Knowledge Infrastructure). We used pooled odds ratio (OR) and 95% confidence interval (95% CI) to evaluate the strength of the association in both standard and cumulative meta-analysis. Subgroup and sensitivity analysis was also performed, and between-study heterogeneity and publication bias were checked.@*RESULTS@#A total of seventeen studies referring to three AhR polymorphisms (rs2066853, rs7796976, and rs2074113) were identified, and 9557 cases and 10038 controls were included. There was no statistically significant association of AhR rs2066853 polymorphism with cancer risk in the overall population, and the negative results were repeated in subgroup analysis by the ethnicity and cancer type. Concerning AhR rs7796976 or rs2074113 polymorphism, no significant correlation was detected. Moreover, these non-significant findings were stable in sensitivity analysis, and the cumulative meta-analysis indicated a trend of no significant link between this three AhR polymorphisms and cancer risk as more data accumulated over time.@*CONCLUSION@#This meta-analysis provides evidence that the rs2066853, rs7796976, or rs2074113 polymorphism in AhR gene is not a susceptible predictor of cancer. Further clinical and functional investigation between AhR polymorphisms and cancer susceptibility are needed.


Subject(s)
Basic Helix-Loop-Helix Transcription Factors/genetics , Confidence Intervals , Genetic Predisposition to Disease/epidemiology , Humans , Neoplasms/genetics , Odds Ratio , Polymorphism, Genetic , Receptors, Aryl Hydrocarbon/genetics
18.
Article in Chinese | WPRIM | ID: wpr-827190

ABSTRACT

OBJECTIVE@#To explore the clinical application value of serum light chain (sLC) in the diagnosis and therapeutic efficacy evaluation for multiple myeloma.@*METHODS@#46 patients with newly diagnosed multiple myeloma were selected as MM group and 50 healthy persons as control group. Rate scattering immunoturbidimetry was used to detect serum light chain and immunoglobulin (Ig) in two groups, serum protein electrophoresis was used to detect M protein by agarose gel. Then, the sensitivity and specificity of the two methods in MM diagnosis were analyzed and compared, and the significance of sLC detection in MM diagnosis were discussed. In addition, 15 MM patients after received conventional therapy were tracked, sLC levels in five different therapentic times were recorded, and the effect of sLC in efficacy evaluation of MM was analyzed.@*RESULTS@#There were 11 cases of IgA type, 15 cases of IgG type, 8 cases of light chain κ type, 8 cases of light chain λ type, 2 cases of IgD type, and 2 cases of non-secretion type. The sLC-κ, sLC-λ and their ratio (including light chain type and double clone type), IgA and IgG (except IgD type), as well as albumin, beta-globulin and gamma-globulin levels showed statistically significant differences (P<0.05) compared with the control group. The sensitivity of serum protein electrophoresis, Ig quantification, sLC and its ratio in the diagnosis of multiple myeloma were 57%, 76% and 65%, and their specificity were 83%, 61% and 90%, respectively. After the second or third chemotherapy, the sLC-κ/λ ratio gradually approached the normal range as the disease reliefes, and the sLC-κ/λ ratio continued to be on or off the line at outliers or further away from the reference value as the disease progresses in MM patients with κ type or λ type.@*CONCLUSION@#sLC detection shows positive significance in early diagnosis of multiple myeloma, SLC monitoring can be used for the efficacy evaluation for treatment of MM patients.


Subject(s)
Humans , Immunoglobulin kappa-Chains , Immunoglobulin lambda-Chains , Multiple Myeloma , Diagnosis
19.
Article in Chinese | WPRIM | ID: wpr-825687

ABSTRACT

Objective To estimate the current status of the epidemic of acquired immunodeficiency syndrome (AIDS) in Wuhan in 2017, and to provide a scientific basis for developing HIV/AIDS prevention strategies. Methods A Workbook model was used to estimate the 2017 HIV/AIDS epidemic in Wuhan using Wuhan demographic data and HIV/AIDS epidemiological data. The Chi-square test was used to compare the differences in gender and transmission routes between the epidemic estimates and the case report data. Results It was estimated that there were 6,008 surviving adult HIV/AIDS cases in Wuhan in 2017. The estimated prevalence of HIV infection in Wuhan was 0.064% in 2017. The estimated detection rate for HIV/AIDS was 75.0%. Among the 6,008 adult HIV/AIDS cases, 4,017 (66.9%) were transmitted through homosexual behaviors, 1,803 (30.0%) were transmitted through heterosexual behaviors, and 188 (3.1%) were transmitted through injecting drugs. The estimated detection rates of HIV/AIDS cases infected by homosexual behavior, heterosexual behavior and drug injection were respectively 73.89%, 78.81% and 48.94%. Conclusion The HIV/AIDS epidemic in Wuhan was still in a low prevalence state, but nearly a quarter of the infected HIV/AIDS cases had not been detected yet. Homosexual behaviors contributed most to HIV/AIDS epidemic in Wuhan, and heterosexual behaviors among spouse/fixed sexual partners also accounted for a certain proportion of HIV/AIDS epidemic. In order to further improve HIV detection rate and curb HIV transmission, it is necessary to explore more effective HIV monitoring and testing practices to detect HIV/AIDS cases as early as possible, especially among high-risk populations and their sexual partners.

20.
Acta Pharmaceutica Sinica ; (12): 1849-1854, 2020.
Article in Chinese | WPRIM | ID: wpr-825159

ABSTRACT

The aim of the present study was to determine the metabolic changes and possible toxic mechanisms of ketamine-associated bladder toxicity. Twenty-four male Sprague-Dawley (SD) rats were randomly allocated into a control group, a low-dose group and a high-dose group. The behavior of these rats was observed every day. In addition, the weight, 2 h urinary frequency and organ coefficient of the bladder were measured. Serum IL-6 and TNF-α levels were measured using an enzyme-linked immunosorbent assay (ELISA). Urinary metabolites were analyzed using gas chromatography-mass spectrometry (GC-MS). This research was approved by the Ethics Committee of the Animal Experiment Center of Southwest Medical University (No. 201901-98). After 12 weeks of administration, the frequency of 2 h urination and the bladder mass index were significantly different in the low-dose and high-dose groups compared with the control group. Serum IL-6 and TNF-α levels were higher than those of the control group (P<0.05). Bladder HE staining showed that long-term administration of ketamine could induce cystitis. The concentrations of the three common differential metabolites, including 3-aminoisobutyric acid, citric acid and uric acid in the low-dose and the high-dose groups were increased compared with those in the control group. This study indicates that 3-aminoisobutyric acid, citric acid and uric acid and their related metabolic pathways may be closely related to ketamine-associated bladder toxicity.

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