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Chinese Journal of Epidemiology ; (12): 1005-1009, 2003.
Article in Chinese | WPRIM | ID: wpr-246416

ABSTRACT

<p><b>OBJECTIVE</b>The strains of Yersinia pestis isolated in different period and different natural foci in China were analyzed.</p><p><b>METHODS</b>Traditional and molecular biological methods were used. Rhamnose fermentation, rRNA gene copy number, nitrite reduction, and the glycerol fermentation were important characters for typing, and pulse field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) profile could reflect the genetic distance between the strains.</p><p><b>RESULTS</b>The strains could be divided into 15 genetic types by those 6 characters with each of them covered an isolated geographical territories.</p><p><b>CONCLUSION</b>The characters of strains were described; the genetic relationship of different types, their evolution, and the forming and shift of plague natural foci were analyzed.</p>


Subject(s)
China , Databases, Genetic , Electrophoresis, Gel, Pulsed-Field , Genetic Drift , Geography , Mutation , Random Amplified Polymorphic DNA Technique , Yersinia pestis , Classification , Genetics
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