Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 23
Filter
1.
Article in Chinese | WPRIM | ID: wpr-878933

ABSTRACT

CNKI, PubMed and other databases were retrieved to extract eligible randomized controlled trial(RCT) about modified Xuefu Zhuyu Decoction(MXZD) combined with Western medicine(trial group) versus Western medicine alone(control group) in the treatment of leiomyoma. Therefore, a total of 25 RCTs were included, involving 2 328 patients. Bias risk evaluation tool in Cochrane Handbook 5.1.0 was used for evaluating the quality of these RCTs. Meta-analysis was performed for the reported indicators, including total efficiency, serum hormone level [progesterone(P), luteinizing hormone(LH), estradiol(E_2), follicle stimulating hormone(FSH)], uterine size, fibroids size and adverse reactions by using Stata 14.0 software. Meta-analysis showed that the total efficiency(RR=1.21,95%CI[1.17,1.25],P<0.05) of trial group was better than that of control group. Serum hormone level(WMD_P=-3.86,95%CI[-4.31,-3.41],P<0.05; WMD_(LH)=-3.64,95%CI[-4.47,-2.82],P<0.05; WMD_(E_2)=-39.99,95%CI[-53.45,-26.52],P<0.05; WMD_(FSH)=-3.79,95%CI[-4.86,-2.72],P<0.05), uterine size(WMD=-50.02,95%CI[-55.98,-44.06],P<0.05), fibroids size(WMD=-15.79,95%CI[-18.11,-13.46],P<0.05) and adverse reactions(RR=0.65,95%CI[0.48,0.88],P<0.05) of trial group were all lower than those of control group, with statistical significances. Trial sequential analysis(TSA) was performed by using TSA 0.9 software, and showed a reliable therapeutic effect of the experimental group. In short, our study indicated that modified Xuefu Zhuyu Decoction combined with Western medicine had a better therapeutic effect on leiomyoma than Western medicine alone, but more high-quality studies are needed to verify this conclusion in the future.


Subject(s)
Drugs, Chinese Herbal , Humans , Leiomyoma/drug therapy , Medicine
2.
Chinese Pharmaceutical Journal ; (24): 580-587, 2020.
Article in Chinese | WPRIM | ID: wpr-857725

ABSTRACT

OBJECTIVE: To study and compare the HPLC fingerprints of different polar parts of Achyranthes bidentata of different specifications, investigate their internal quality differences, and provide reference for the standard improvement and clinical use of the medicinal material. METHODS: The Achyranthes bidentata samples were refluxed with 75% ethanol, the extract was dissolved with 40 mL water and then,extracted in turn with petroleum ether, chloroform, ethyl acetate and n-butanol, each extracted phase and aqueous phase were obtained and concentrated. The HPLC fingerprint of each part was collected. The data was analyzed by similarity and comprehensive clustering. Meanwhile, the fingerprints of different parts were compared and analyzed. RESULTS: Eleven common peaks were determined in the HPLC fingerprints of petroleum ether fraction and chloroform extract fraction, 10,19,and 8 common peaks were determined in the HPLC fingerprints of ethyl acetate fraction, n-butyl alcohol fraction,and water extract respectively. The similarity of the fingerprints of chloroform parts was quite low.The similarity of the fingerprints of other parts was higher,all above 0.9. The difference in the fingerprints of petroleum ether parts was mainly reflected in the peak height. The chemical compositions and peak heights of ethyl acetate, chloroform and water extracts were different. Comprehensive cluster analysis could distinguish different specifications of Achyranthes bidentata. CONCLUSION: The internal qualities of different specifications of Achyranthes bidentata are different. HPLC fingerprint established in this experiment can comprehensively reflect the chemical components distribution of Achyranthes bidentata of different specifications. It provides reference for the whole quality evaluation of different specifications of Achyranthes bidentata.

3.
Article in English | WPRIM | ID: wpr-878305

ABSTRACT

Objective@#Several COVID-19 patients have overlapping comorbidities. The independent role of each component contributing to the risk of COVID-19 is unknown, and how some non-cardiometabolic comorbidities affect the risk of COVID-19 remains unclear.@*Methods@#A retrospective follow-up design was adopted. A total of 1,160 laboratory-confirmed patients were enrolled from nine provinces in China. Data on comorbidities were obtained from the patients' medical records. Multivariable logistic regression models were used to estimate the odds ratio ( @*Results@#Overall, 158 (13.6%) patients were diagnosed with severe illness and 32 (2.7%) had unfavorable outcomes. Hypertension (2.87, 1.30-6.32), type 2 diabetes (T2DM) (3.57, 2.32-5.49), cardiovascular disease (CVD) (3.78, 1.81-7.89), fatty liver disease (7.53, 1.96-28.96), hyperlipidemia (2.15, 1.26-3.67), other lung diseases (6.00, 3.01-11.96), and electrolyte imbalance (10.40, 3.00-26.10) were independently linked to increased odds of being severely ill. T2DM (6.07, 2.89-12.75), CVD (8.47, 6.03-11.89), and electrolyte imbalance (19.44, 11.47-32.96) were also strong predictors of unfavorable outcomes. Women with comorbidities were more likely to have severe disease on admission (5.46, 3.25-9.19), while men with comorbidities were more likely to have unfavorable treatment outcomes (6.58, 1.46-29.64) within two weeks.@*Conclusion@#Besides hypertension, diabetes, and CVD, fatty liver disease, hyperlipidemia, other lung diseases, and electrolyte imbalance were independent risk factors for COVID-19 severity and poor treatment outcome. Women with comorbidities were more likely to have severe disease, while men with comorbidities were more likely to have unfavorable treatment outcomes.


Subject(s)
Adult , Aged , COVID-19/virology , China/epidemiology , Comorbidity , Female , Humans , Male , Middle Aged , Retrospective Studies , Severity of Illness Index , Treatment Outcome
4.
Chinese Journal of Microsurgery ; (6): 236-238, 2018.
Article in Chinese | WPRIM | ID: wpr-711659

ABSTRACT

Objective To explore the emergency strategy of microsurgical replantation to open degloving injuries in plantar soft tissue.Methods From July,2013 to July,2017,5 patients with plantar degloving injury were treated by micro-replantation,3 of which were injuried by traffic accident and 2 caused by crashed injury.After early debridement,4 cases received anastomosis of blood vessels,and 1 treated with Venous Autograft to get recovery of blood circulation.The avulsion flaps were sutured without tension.Results Four flaps survived after plantar replantation,and 1 developed a partial necrosis and secondery repaird with skin graft.The plantar repair made sensory function preserved in the maximum extent.All patients were followed-up for 0.5-2.0 years.The appearance,elasticity and feel of flaps was good.Conclusion Complete debridement,early recovery of blood supply and maximum preservation of plantar tissue function should be the key to successful replantation of open plantar degloving injuries.

5.
China Medical Equipment ; (12): 142-145, 2018.
Article in Chinese | WPRIM | ID: wpr-706530

ABSTRACT

Objective: To establish a method of liquid chromatography-atomic fluorescence spectrometry (LC-AFS), and carry out uncertainty analysis of detecting methyl mercury in fish tissue. Methods: LC-AFS method was adopted to determine the content of methyl mercury in fish tissue. And the theory of assessment and expression about uncertainty measurement based on JJF1059.1-2012 of State Administration for Market Regulation was applied to analyze the source of factors that affect its uncertainty. Through evaluated various factors of uncertainty and calculated and combined uncertainty to obtain the extended uncertainty of measurement results. Results: The content of methyl mercury in fish tissue by using LC-AFS wasω=(817.94±105.02)μg/kg (k=2, confidence level was 95%). Conclusion: The measurement uncertainty assessment can be used in the uncertainty analysis that LC-AFS measure content of methyl mercury in fish tissue. Therefore, the results are more reliable.

6.
Article in Chinese | WPRIM | ID: wpr-700064

ABSTRACT

Object To migrate and reconstruct the hospital network to solve its problems in obsolete equipment, network architecture, software configuration and etc.Methods A network migration and reconstruction scheme was proposed based on the physical environment of one hospital with two addresses as well as some network technologies such as open shortest path first (OSPF) protocol, port trunking and stacking.Results The network migration and reconstruction were implemented as desired, and the core switching network was upgraded to ten gigabit Ethernet.Conclusion The hospital network is optimizedin architecture, software configuration, reliability and performances, and lays a foundation for hospital informatization.

7.
Article in Chinese | WPRIM | ID: wpr-703558

ABSTRACT

The hierarchical diagnosis and treatment system is an important measure for rational allocation of medical resources, promotion of equalization of basic medical services, improving the patients' disorderly medical treatment and guiding patients' hospital choice behavior. This paper defines the utility function of rational medical treatment of patients affected by multiple factors such as the personal disposable income,the age,the severity of the self-induced illness and the decision-makers' education level, and considered the outpatient price, service capacity and other factors of medical institutions. The present study constructed the incomplete information dynamic game model based on fictitious play method. It provided the quantitative analysis of the statistical data of Qinhuangdao and obtained the patients distribution in medical institution. The rationality of the model was verified by comparing the simulation with actual data,and showed the choice is more reasonable between the primary health care and general hospital. Therefore,the emphasis of optimizing the medical service resources in hierarchical diagnosis and treatment system is to establish the treatment system of integrated medical service for patients'on-demand and on-demand medi-cal services. At the same,various incentives should be used to enhance patients'enthusiasm for primary health care.

8.
Article in Chinese | WPRIM | ID: wpr-703557

ABSTRACT

The paper analyzed the decision-making process of the doctor's treatment and patient's decision-making. Then established the evolutionary game model of patient's and the secondary-tertiary hospital doctor's strate-gy. The Matlab simulation software were used to analyze the influencing factors of patient-doctor strategy-making sys-tem. We proposed the advice reducing critical patient's utility loss diagnosed in secondary hospital,improving medi-cal experience of common patient in secondary hospital,strengthening disease prevention of critical patient,and pro-moting labor division and cooperation between secondary and tertiary hospital to make result develop towards reduction the disorderly,chaotic and dislocated medical need and an integrated secondary and tertiary system.

9.
Article in Chinese | WPRIM | ID: wpr-659410

ABSTRACT

Nowadays,some new characteristics emerge in the modem management of severe acute pancreatitis (SAP),such as minimally invasive,staging,multidisciplinary,specialized and diversified.There is a prevalent insufficiency in the surgical management of SAP due to various reasons.A precise grasp of timing,indication and manner in the surgical management of SAP is of great importance to further improve the curative rate.Surgical intervention of SAP is performed in a staged step-up framework,which should not only be indicated by infectious complications in the late-phase of SAP,but also by a failure of nonsurgical therapy and a progression to abdominal compartment syndrome and/or multiple organ failure (MOF) in the earlyphase of SAP.In a staged step-up framework,imaging-guided percutaneous catheter drainage could be established relatively early,MOF and heterogeneous collection are independent risk factors of the necessity of surgical debridement,and the merits of open debridement should not be under-estimated.A sameadmission cholecystectomy could be arranged for patients with gallstone SAP,and laparoscopic cholecystectomy serves as the initial choice.In the context of minimal-invasiveness and diversification,the surgeons should endeavor to establish their predominant role in the multidisciplinary team,preventing from insufficient surgical intervention in the management of SAP.

10.
Article in Chinese | WPRIM | ID: wpr-657398

ABSTRACT

Nowadays,some new characteristics emerge in the modem management of severe acute pancreatitis (SAP),such as minimally invasive,staging,multidisciplinary,specialized and diversified.There is a prevalent insufficiency in the surgical management of SAP due to various reasons.A precise grasp of timing,indication and manner in the surgical management of SAP is of great importance to further improve the curative rate.Surgical intervention of SAP is performed in a staged step-up framework,which should not only be indicated by infectious complications in the late-phase of SAP,but also by a failure of nonsurgical therapy and a progression to abdominal compartment syndrome and/or multiple organ failure (MOF) in the earlyphase of SAP.In a staged step-up framework,imaging-guided percutaneous catheter drainage could be established relatively early,MOF and heterogeneous collection are independent risk factors of the necessity of surgical debridement,and the merits of open debridement should not be under-estimated.A sameadmission cholecystectomy could be arranged for patients with gallstone SAP,and laparoscopic cholecystectomy serves as the initial choice.In the context of minimal-invasiveness and diversification,the surgeons should endeavor to establish their predominant role in the multidisciplinary team,preventing from insufficient surgical intervention in the management of SAP.

11.
Article in Chinese | WPRIM | ID: wpr-479789

ABSTRACT

Objective To investigate cognitive status and influencing factors for hand hygiene(HH)among health care workers(HCWs),and provide basis for scientific management of HH.Methods In April 2013,HCWs in a general hospital were selected by randomly sampling method,questionnaires were used to survey the implementation of HH in recent one month and HCWs’cognition on knowledge about HH.Results A total of 750 HCWs were in-vestigated,652 available questionnaires were collected.The frequency of hand washing and hand disinfection per day among most HCWs were 10 - 19 times,accounting for 46.62% and 47.85% respectively;30.52% of HCWs washed their hands for ≥30 seconds each time,60.58% of HCWs dried hands with paper towel after washing hands,57.21 % of HCWs abided by six-step hand washing method.The overall correct rate of cognition on ten op-portunities that requiring HH in clinical practice was 68.68%.The main factors influencing the implementation of HH were as follows:skin irritation of hand sanitizer and hand disinfectant subjectively considered by HCWs (63.34%),inadequate hand washing facilities(41 .10%);high cost of hand sanitizer ,hand disinfectant,and dry paper towel (38.96%),et al.Conclusion In addition to intensifying education on HH,installing rational HH facili-ties and improving HH standard are key points in strengthening HH in general hospital.

12.
Chinese Journal of Surgery ; (12): 653-656, 2015.
Article in Chinese | WPRIM | ID: wpr-308503

ABSTRACT

Both new insights in the pathophysiology of severe acute pancreatitis (SAP) and upspringing related evidence-based supports prompt the staged step-up approach, which stress emphasis on minimal invasiveness and damage control, to be accepted and advocated by the majority of guidelines. For documented or suspected patients with infected pancreatic necrosis, an imaging-guided percutaneous catheter drainage or an endoscopic transluminal drainage should be initially performed followed by, if necessary, a minimal access retroperitoneal necrosectomy, or a video-assisted retroperitoneal debridement, or an endoscopic transluminal necrosectomy, or an even an open access necrosectomy. The outstanding performance of staged step-up approach in patients with SAP has been justified from both a clinical and a health economic point of view, meanwhile, there are some issues remained to be further elucidated and optimized.


Subject(s)
Acute Disease , Debridement , Drainage , Endoscopy , Humans , Pancreatitis , General Surgery , Retroperitoneal Space , Treatment Outcome
13.
Article in Chinese | WPRIM | ID: wpr-444029

ABSTRACT

Objective To analyze the results of surgical treatment on chronic pancreatitis(CP),and investigate how to choose the appropriate surgical procedure.Methods The clinical data of 54 patients with chronic pancreatitis who underwent surgery at the First Affiliated Hospital of Harbin Medical University from January 2007 to December 2011 were retrospectively analyzed.Results All the 54 patients underwent surgery,including 8 decompression and drainage procedure (Partington procedure) ; 13 resections (7 cases of pancreaticoduodenectomy,1 case of distal pancreatectomy,4 cases of distal pancreatectomy combined with splenectomy and 1 case of pancreaticoduodenectomy combined with distal pancreatectomy,respectively); 12hybrids (7 cases of Beger procedure and 5 cases of Frey procedure,respectively) and 21 other procedures (15 cases of pancreatic pseudocyst jejunostomy,4 cases of exploratory laparotomy combined with pancreatic tissue biopsy,2 cases of gastrojejunostomy combined with choledochojejunostomy,respectively).There were 4 cases of post-operative pathologic evidence of cancer.Twelve patients had postoperative complications and were cured with non-operative management.Forty-four patients (81.5%) were followed for 2 to 67 months,36 out of 42(85.7%) patients who suffered from abdominal pain had a persistent remission,there were one case of new on-set diabetes and no steatorrhea was reported.Conclusions For CP patients with surgical indications,the choice of procedure should be individualized for the purpose of preserving the endocrine and exocrine functions of pancreas,and taking effectiveness as well as safety into consideration.

14.
Article in Chinese | WPRIM | ID: wpr-327855

ABSTRACT

We revisited the "Xiao Chaihu Decoction event (XCHDE)" occurred in late 1980s in Japan and the Bupleuri Radix related adverse drug reaction (ADR) reports in China After careful review, comparison, analysis and evaluation, we think the interstitial pneumonitis, drug induced Liver injury (DILI) and other severe adverse drug envents (ADEs) including death happened in Japan is probably results from multiple factors, including combinatory use of XCHDE with interferon, Kampo usage under modern medicine theory guidance, and use of XCHD on the basis of disease diagnosis instead of traditional Chinese syndrome complex differentiation. There are less ADE case reports related to XCHD preparation in China compared to Japan, mostly manifest with hypersensitivity responses of skin and perfuse perspiration. The symptoms of Radix Bupleuri injection related ADEs mainly manifest hypersensitivity-like response, 2 cases of intravenous infusion instead of intramuscular injection developed hypokalemia and renal failure. One case died from severe hypersensitivity shock. In Chinese literatures, there is no report of the interstitial pneumonitis and DILI associated with XCHDG in Japan. So far, there is no voluntary monitoring data and large sample clinical research data available. The author elaborated the classification of "reevaluation" and clarified "re-evaluation upon events" included the reaction to the suspected safety and efficacy events. Based on the current status of the clinical research on the Radix Bupleuri preparations, the author points out that post-marketing "re-evaluation upon suspected event" is not only a necessity of continuous evaluation of the safety, efficacy of drugs, it is also a necessity for providing objective clinical research data to share with the international and domestic drug administrations in the risk-benefit evaluation. It is also the unavoidable pathway to culture and push the excellent species and famous brands of TCM to the international market, in that it enhances the pharmaceutical manufactures the ability to control enterprise risk and eliminate the negative impact on traditional Chinese medicine. With the widely used and ADR report concentrated Radix Bupleuri injection as an example, the author provide a new approach to post-marketing clinical research.


Subject(s)
Bupleurum , Chemistry , Drug Compounding , Drug-Related Side Effects and Adverse Reactions , Drugs, Chinese Herbal , Humans , Product Surveillance, Postmarketing , Methods , Safety
15.
Article in Chinese | WPRIM | ID: wpr-431144

ABSTRACT

Objective To summarize the experience in surgical management of hilar cholangiocarcinoma.Methods The clinical data of 88 patients with hilar cholangiocarcinoma who received surgical treatment at the First Affiliated Hospital of Harbin Medical University from January 2007 to December 2011 were retrospectively analyzed.All the patients were diagnosed by imaging examination.According to the severity of jaundice and predictive remnant liver volume,19 patients received percutaneous transhepatic cholangial drainage (PTCD) and 4 received portal vein embolization.The fundamental operation consisted of hilar cholangiocarcinoma resection,skeletonization of hepatoduodenum ligament and Roux-en-Y cholangiojejunostomy,and the transanastomotic stent was placed for 6 months.The count data were analyzed using the chi-square test; the survival rate was analysed using the Kaplan-Meier method; the survival was analyzed using the Log-rank test.Results Of the 88 patients,58 patients (including 11 patients who received PTCD) received hilar cholangiocarcinoma resection.Of the 58 patients,43 (including 4 patients who received portal vein embolization preoperatively) received R0 resection,and 15 received palliative resection.Thirty patients received internal and (or) external drainage.Commitant partial hepatectomy was performed on 22 patients (including 9 received left hemihepatectomy,2 received extended left hemihepatectomy,7 received left hemihepatectomy + caudate lobectomy,4 received right hemihepatectomy).Commitant pancreatico-duodenectomy was performed on 7 patients,commitant hepatic artery resection on 3 patients,and commitant portal vein resection on 2 patients.According to the modified Bismuth-Corlette classification,there were 17 patients with type Ⅰ,19 with type Ⅱ,21 with type Ⅲa,20 with type Ⅲb,and 11 with type Ⅳ.Of the 58 patients who received hilar cholangiocarcinoma resection,19 had postoperative complications,and 2 patients died within 30 days after operation.Seventy-three patients were followed up,and the overall 1-,3-,5-year survival rates were 68.5%,28.8%,11.0%,respectively.The 1-,3-,5-year survival rates of patients who received R0 resection were 94.6%,43.2%,18.9%,respectively,which were significantly higher than 78.6%,35.7% and 7.1% of patients who received palliative resection (x2=4.77,P <0.05).The 1-,3-,5-year survival rates of patients who received palliative resection were significantly higher than 18.2%,0,0 of patients who received biliary drainage (x2 =13.26,P < 0.05).Conclusions R0 resection is the best choice for patients with hilar cholangiocarcinoma,and biliary drainage with no resection is the last choice.Sufficient preoperative treatment,optimized choice of surgical procedure and exquisite surgical techniques are important for the improvement of the prognosis.

16.
Article in Chinese | WPRIM | ID: wpr-291282

ABSTRACT

By comprehensive review and analysis of post-marketing clinical research on the efficacy and safety,we concluded that Fufang Zaoren capsule has certain therapeutic effects for insomnia, although current clinical research design needs improving. The post-marketing clinical studies also showed that it causes several adverse reactions at the recommended doses, such as chills, fever, dizziness, nausea, shortness of breath, chest tightness and palpitations, whereas high doses of Fufang Zaoren capsule can cause delayed extrapyramidal symptoms. Health Canada government website also prompted the L-tetrahydropalmatine in Fufang Zaoren capsule caused liver damage in pregnant women. The authors summarized the risk points, factors and risk control in the clinical use of Fufang Zaoren capsule and also present their perspective on the research status, existing problems and corresponding countermeasures in the post-marketing clinical re-evaluation of traditional Chinese medicine.


Subject(s)
Capsules , Economics , China , Drug Evaluation , Economics , Drugs, Chinese Herbal , Economics , Female , Humans , Male , Marketing , Product Surveillance, Postmarketing , Economics
17.
Chinese Journal of Burns ; (6): 451-455, 2011.
Article in Chinese | WPRIM | ID: wpr-257828

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of local injection of insulin on the level of systemic blood glucose and granulation tissue formation of wound in patients with diabetic foot ulcer.</p><p><b>METHODS</b>Thirty-two patients with diabetic foot ulcer hospitalized in our wards from June 2009 to June 2010 were divided into insulin (I, n = 16) and control (C, n = 16) groups according to the random number table. For patients in I group, after debridement, one half of calculated dose of insulin diluted with equal amount of normal saline was injected diffusely into the base of the ulcer, and another half dose of insulin was subcutaneously injected into abdominal wall for 7 days, two times a day. For patients in C group, after debridement, primary insulin was subcutaneously injected into abdominal wall, 1 mL saline was subcutaneously injected into basal layer of ulcer for 7 days, two times a day. Before injection and 0.5, 1.0, 2.0, and 4.0 hours after injection (PIH), level of fasting blood glucose was determined. Before injection and on post injection day (PID) 3, 5, and 7, the growth of granulation tissue was assessed, and wound specimens were harvested for observation of CD34 expression and calculation of microvessel density (MVD). Data were processed with t test.</p><p><b>RESULTS</b>The levels of fasting blood glucose in both groups during observational time points ranged from 6.6 mmol/L to 12.8 mmol/L with a mean of (10.0 ± 2.2) mmol/L, and there was no statistical difference (with t values from 0.000 to 2.209, P values all above 0.05). Growth of granulation tissue in I group was more exuberant from PID 5, especially on PID 7 [(59.06 ± 1.58)%], which was significantly richer than that in C group [(23.61 ± 1.57)%, t = 17.420, P = 0.000]. New vessels were observed in I group from PID 3 as indicated by CD34 expression. There was no obvious difference in the number of MVD between I group and C group on PID 3 (t = 0.247, P > 0.05). The number of MVD per 200 times visual field in I group was respectively 8.34 ± 0.48, 11.22 ± 0.97 on PID 5 and 7, which was respectively higher than that in C group (4.42 ± 0.14, 5.44 ± 1.13, with t value respectively 16.568, 27.664, P values all below 0.01).</p><p><b>CONCLUSIONS</b>Local injection of insulin has a significant effect on systemic blood glucose in patients with diabetic foot ulcer, and it can promote the growth of granulation tissue and wound healing.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Blood Glucose , Metabolism , Diabetic Foot , Drug Therapy , Metabolism , Female , Humans , Injections , Insulin , Therapeutic Uses , Male , Middle Aged , Wound Healing
18.
Article in Chinese | WPRIM | ID: wpr-275709

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of necrostatin (Nec-1) on apoptosis induced by aluminum (Al), and approach the mechanism.</p><p><b>METHODS</b>Neural cell death model was made by 4 mmol/L Al treated neuroblastoma cells (SH-SY5Y). Cell viabilities were detected at different concentrations of Al and/or Nec-1. Hoechst 33342/PI double staining was used to observe apoptosis and (or) necrosis that were quantified by flow cytometry using Annexin V/PI double staining. Apoptotic pathway was tested by activities of Caspase-3, Caspase-8 and Caspase-9. In addition, the expression of NF-kappa B and Cyt-c was measured by immunocytochemistry.</p><p><b>RESULTS</b>Cell viabilities were significantly decreased with the increasing concentrations of Al (P < 0.05), which could be significantly upregulated by 60 micromol/L Nec-1 (P < 0.05) and were correlated with the concentrations of Nec-1 (P < 0.05, P < 0.01). Apoptosis and necrosis were observed under fluorescent microscope and quantified by flow cytometry, which suggested an increasing trend of apoptotic and necrotic rates (P < 0.05, P < 0.01). Whereas, Nec-1 could not only decrease the necrotic rate but also apoptotic rate as well (P < 0.05, P < 0.01). Data of Nec-1 on caspases activities showed that Nec-1 could not affect Caspase-9 activity (P > 0.05) and Cty-c protein expression as well (P > 0.05). However, Nec-1 could reduce Caspase-8 activity significantly (P < 0.05, P < 0.01) and increase NF-kappa B protein expression (P < 0.05, P < 0.01) and finally decrease Caspase-3 activity (P < 0.05).</p><p><b>CONCLUSION</b>Nec-1 could reduce cell apoptosis induced by Al, through Caspase-8 pathway, and up-regulate the expression of NF-kappa B protein.</p>


Subject(s)
Aluminum , Toxicity , Apoptosis , Caspase 3 , Metabolism , Caspase 8 , Metabolism , Caspase 9 , Metabolism , Cell Death , Cell Line, Tumor , Cell Survival , Cytochromes c , Metabolism , Humans , Imidazoles , Pharmacology , Indoles , Pharmacology , NF-kappa B , Metabolism , Neuroblastoma
19.
Chinese Medical Journal ; (24): 2781-2785, 2010.
Article in English | WPRIM | ID: wpr-237416

ABSTRACT

<p><b>BACKGROUND</b>Population based epidemiologic study on the main diseases and birth status of liveborn neonates remains scarce in China, especially in rural areas where a large number of neonates are born. The aim of this study was to establish an epidemiological basis of live births in Julu County, a representative of the northern and mid-western parts of China in terms of demography, disease pattern and women and children's health care infrastructure.</p><p><b>METHODS</b>The perinatal data of all live births were prospectively collected in three participating county-level hospitals from September 1, 2007 to August 30, 2008.</p><p><b>RESULTS</b>There were 5822 live births in these hospitals. Among all live births, 53.7% were male and 4.5% were born prematurely. Mean (SD) birth weight (BW) was (3348 ± 503) g. The low (< 2500 g) and very low BW (< 1500 g) infants accounted for 3.8% and 0.5% of the total births, with 6.5% as small for gestational age and 2.8% as multi-births. Cesarean section rate was 30.2%, of which 68.6% were elective. There were 745 infants (12.8% of the live births) admitted to local neonatal wards within 7 days of postnatal life, in which 48.3% and 19.3% were due to perinatal asphyxia and prematurity, respectively. The incidences of perinatal aspiration syndrome, transient tachypnea and respiratory distress syndrome were 4.9%, 0.6% and 0.5%, respectively. Neonatal mortality was 7.6‰ (44/5822), with 16 in delivery room and 28 in neonatal ward before discharge.</p><p><b>CONCLUSIONS</b>This study provided a population-based perinatal data of live births and neonatal mortality in a northern China county with limited resources. Neonatal disorders related to perinatal asphyxia remain a serious clinical problem, which calls for sustained education of advanced neonatal resuscitation and improvement in the quality of perinatal-neonatal care.</p>


Subject(s)
Asphyxia Neonatorum , Epidemiology , Birth Weight , China , Epidemiology , Female , Humans , Infant Mortality , Infant, Newborn , Infant, Newborn, Diseases , Epidemiology , Therapeutics , Male , Prospective Studies , Respiratory Distress Syndrome, Newborn , Epidemiology , Therapeutics
20.
Article in Chinese | WPRIM | ID: wpr-315707

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of benzo[a]pyrene (B[a]P) on capability of learning and memory and the content of amino acid neurotransmitters in hippocampus of rats.</p><p><b>METHODS</b>Thirty-two healthy, male SD rats were randomly divided into 4 groups according to their weights after intubated into ventricles: the solvent control group, 2.5, 5.0 and 10.0 mmol/L groups. 10 microl of B[a]P olive oil solutions, of different concentrations 2.5, 5.0 and 10.0 mmol/L, were injected into rats' lateral ventricles, respectively. Rats in the solvent control group were injected into the same volume of olive oil as that in B[a]P group. Rats' capability of learning and memory was tested by Morris water maze. The content of amino acid neurotransmitters in rats' hippocampus were determined by high performance liquid chromatogram with a fluorescence detector.</p><p><b>RESULTS</b>Compared with the controls, the performances of learning and memory of rats decreased significantly in B[a]P treated groups (P<0.01). Levels of glutamate (Glu) were lower significantly in treated groups than that in controls (P<0.01). No significant differences were found in contents of aspartic acid (Asp), glycine (Gly) and aminobutyric acid (GABA) among the four groups.</p><p><b>CONCLUSION</b>B[a]P can damage rats' spatial learning and memory, and which could be related to decreased contents of excitatory amino acids in hippocampus.</p>


Subject(s)
Amino Acids , Metabolism , Animals , Benzo(a)pyrene , Toxicity , Hippocampus , Metabolism , Male , Maze Learning , Memory , Neurotransmitter Agents , Metabolism , Rats , Rats, Sprague-Dawley
SELECTION OF CITATIONS
SEARCH DETAIL