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1.
Article in Chinese | WPRIM | ID: wpr-333615

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of bafilomycin A1 (BAF) on the cell proliferation, invasiveness, apoptosis, and oxaliplatin sensitivity in gastric cancer MGC-803 cells.</p><p><b>METHODS</b>MGC-803 cells were divided into control group, BAF group, oxaliplatin group, and BAFµ oxaliplatin group. MTT assay and plate clone formation assay were used to assess the viability and colony forming ability of the cells after the treatments. The expression of nucleosomes in the cells was examined with ELISA. The cell migration and invasion after the treatments were evaluated. Western blotting was performed to detect the expression of Bcl-2 and Bax in the treated cells, and scanning electron microscopy, immunohistochemistry and Western blotting were employed to to observe the cell autophagy.</p><p><b>RESULTS</b>Compared with the control cells, the cells treated with BAF showed a substantial decrease in autophagosome accumulation with attenuated cell proliferation, migration and invasion. Compared with cells treated with oxaliplatin alone, the cells treated with both BAF and oxaliplatin showed significantly lowered autophagosome accumulation, suppressed cell proliferation, migration and invasion, increased cell apoptosis, increased Bax expression and lowered Bcl-2 expression.</p><p><b>CONCLUSION</b>BAF can inhibit the proliferation and invasiveness of MGC-803 cells, promote cell apoptosis by inhibiting autophagy, and enhances the sensitivity of the cells to oxaliplatin.</p>


Subject(s)
Apoptosis , Autophagy , Cell Line, Tumor , Cell Movement , Cell Proliferation , Drug Resistance, Neoplasm , Humans , Macrolides , Pharmacology , Organoplatinum Compounds , Pharmacology , Stomach Neoplasms , Pathology
2.
Article in Chinese | WPRIM | ID: wpr-237147

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the risk factors of postoperative complications after reoperation for recurrent Crohn disease(CD).</p><p><b>METHODS</b>From 1995 to 2009, 65 patients undergoing reoperation for recurrent CD were identified in the First Affiliated Hospital of Fujian Medical University. Risk factors of postoperative complications were analyzed. These patients were matched by age to 65 patients undergoing primary operation and treatment outcomes were compared between primary operation and reoperation.</p><p><b>RESULTS</b>Postoperative complications were observed in 25 cases(38.5%) undergoing reoperation for CD recurrence and the rate of postoperative complication was higher than that after primary operation(12.3%). Postoperative complications rate in patients with stoma was significantly lower than those without stoma(15.8% vs. 47.8%, χ(2)=5.831, P=0.016). Compared to primary operation, reoperation had longer operative time, more severe intraperitoneal adhesion, and a longer postoperative hospital stay(all P<0.05).</p><p><b>CONCLUSION</b>Reoperation for CD recurrence is associated with higher postoperative complications. Temporary stoma may decrease the rate of postoperative complication.</p>


Subject(s)
Adult , Aged , Crohn Disease , General Surgery , Digestive System Surgical Procedures , Female , Humans , Male , Middle Aged , Postoperative Complications , Epidemiology , Recurrence , Reoperation , Retrospective Studies , Risk Factors , Young Adult
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