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Pakistan Journal of Pharmaceutical Sciences. 2015; 28 (5): 1595-1600
in English | IMEMR | ID: emr-166648


The aim of this study was to explore the effects of lentinan on the proliferation of human bladder cancer T24 cells and the mechanism regarding the inhibition of cell growth. When gene regulation technique was used to build pcDNA3-TRPM8 expression plasmid, TRPM8 channel activator-lentinan was used for intervention to observe the proliferation of T24 cells. Flow cytometry cell screening method was used to observe the cell ratio of each cell cycle of T24 cells and the ratio of apoptotic and dying cells under the intervention of different concentrations of lentinan using PI single-staining and Annexin V-FITC/PI double-staining. JC-1 and DCFH-DA fluorescence probes were used to observe the influence of different concentrations of lentinan on the mitochondrial membrane potential of T24 cells and intracellular reactive oxygen species [ROS] by confocal microscope. pcDNA-TRPMS plasmid was successfully constructed, and lentinan could inhibit the growth of T24 cells in a dose-dependent pattern. Lentinan played its biological effect through TRPM8 channel to further inhibit the growth of T24 cells, reduced the mitochondrial membrane potential of bladder cancer T24 cell line, and increased the generation of ROS in human bladder cancer T24 cell line. Lentinan led to mitochondrial depolarization or activation of non-mitochondrial pathway to induce intracellular ROS generation, thus eventually inducing T24 cell death and growth inhibition

Apoptosis , Urinary Bladder Neoplasms
Pakistan Journal of Medical Sciences. 2013; 29 (1): 135-139
in English | IMEMR | ID: emr-127053


To investigate the common bacterial resistance of clinical isolates in our hospital in the second half of 2011. Pathogens isolated from clinical samples in the second half of 2011 were analyzed and categorized to perform susceptibility tests. In the gram-negative bacteria, Enterobacteriaceae and non-fermenting gram-negative bacilli accounted for 55.89% and 34.51%. In the gram-positive bacteria, Staphylococcus aureus, Coagulasenegative staphylococci, Enterococcus, Strptococcus pneumonia accounted for 32.85%, 40.39%, 12.41% and 10.22%, respectively. Other species accounted for 4.14%. Klebsiella pneumonia and Pseudomonas aeruginosa were sensitive to cepoperazon, cefepime and imipenem. However, Acinetobacter baumannii was more sensitive to carbapenems antibiotics, which was followed by fourth generation cephalosporins. Klebsiella pneumoniae was extremely sensitive to amikacin, cefepime and imipenem, but was resistant to ampicillin. The detection rates of the broad-spectrum Escherichia coli, Pseudomonasaeruginosa and Klebsiella pneumoniae were 54.51%, 52.08% and 38.65%. The gram negative bacilli were the prevalent clinical pathogens in our hospital in the second half of 2011. The drug resistance of pathogenic bacteria has increased significantly recently, thus the surveillance of antibacterial agents is necessary, and rational use of antibiotic will be urgently needed to reduce the production and dissemination of drug resistant strains

Anti-Bacterial Agents , Gram-Negative Bacteria , Gram-Positive Bacteria
Pakistan Journal of Medical Sciences. 2013; 29 (5): 1199-1202
in English | IMEMR | ID: emr-193694


Objective: To clarify the use of antibiotics in our hospital and to guide the prophylactic use in future hepatobiliary surgical procedures

Methods: A retrospective review of patients who underwent hepatobiliary surgery from January 2011 to June 2011 was included. Data were collected, and surgical site infection [SSI] was defined by the criteria of Center for Disease Control and Prevention. Patients were prescribed antibiotics for the clinical diagnosis of hepatobiliary system diseases

Results: 1564 patients were identified, in which 784 patients [50.13%] did not receive preoperative antibiotic prophylaxis. Of these 355 patients with 784 surgical sites received either preoperative or both preoperative and postoperative antibiotic prophylaxis. The SSI rate of the patients who received prophylaxis alone [2.56%, 20 of 780 sites] was not statistically higher than that of the patients who have not received prophylaxis [2.68%, 21 of 784 sites], and the two groups were not statistically correlated [P=0.77]

Conclusion: The number of the patients who developed SSI was relatively low, and no reduction in the SSI rate was observed among the patients who have received antibiotic prophylaxis

Article in Chinese | WPRIM | ID: wpr-435948


Objective To study the clinical manifestations,diagnosis and surgical treatment of tubular adenoma in the extrahepatic bile duct.Methods The clinical data of 3 patients with tubular adenoma in the extrahepatic bile duct who were admitted to the Affiliated Hospital of Inner Mongolia Medical University from July 2010 to February 2012 were retrospectively analyzed.Results The tumors were located at the middle and lower part of the common bile duct.All the 3 patients received resection of the common bile duct and Roux-en-Y cholangioenterostomy,and recovered well.The results of pathological examination showed that all the patients were with tubular adenoma,and 2 patients had moderate atypical hyperplasia.Conclusions Tubular adenoma in the extrahepatic bile duct could be diagnosed by pathological examination.Radical resection and rerouting of the bile duct can get a better outcome.