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1.
Article in Chinese | WPRIM | ID: wpr-1020841

ABSTRACT

Non-alcoholic fatty liver disease(NAFLD)is the leading cause of chronic liver disease and has posed significant challenges to global public health and medical care.Due to the unclear pathogenesis of NAFLD,lipid accumulation plays a key role in its development.AMPK,as a crucial molecule in lipid metabolism regulation,can improve lipid accumulation caused by NAFLD.This article describes the specific mechanisms of AMPK-related molecules in improving lipid accumulation and treating NAFLD,and lists the current experimental and therapeutic drugs related to AMPK.The potential of AMPK-related drugs in improving lipid accumulation and treating NAFLD is demonstrated,providing ideas and references for future research on AMPK-related drugs for treating NAFLD.

2.
Article in Chinese | WPRIM | ID: wpr-1019843

ABSTRACT

Objective To investigate the effect of Changyuning prescription on PTEN,TLR2,Caspase-3,Caspase-9 in phosphatidylinositol-3 kinase/protein kinase B(PI3K/AKT)signaling pathway in colon tissue of 2,4,6-trinitrobenzenesulfonic acid(TNBS)-induced ulcerative colitis(UC)rats.Methods After adaptive feeding for 7 days,70 SPF rats were divided into blank group of 10 and modeling group of 60.The rats in the modeling group were given TNBS/ethanol solution enema to induce UC model.The rats that successfully bulit the model were randomly divided into model group,mesalazine group,Changyuning high-dose,medium-dose and low-dose groups,and Changyuning high-dose,medium-dose and low-dose groups,respectively,and the equivalent amount of crude drug was 4 g·mL-1,2 g·mL-1,1 g·mL-1 of Changyuning prescription solution 10 mL·kg-1,the mesalamine group was given 10 mL·kg-1 of mesalamine suspension equivalent to 0.2 g·kg-1 crude drug,the blank control group and model group were given an equal volume of 10 mL·kg-1 of normal saline was given by gavage.The rats in each group were given intragastric administration once a day for 14 consecutive days.Rats were sacrificed after the last administration.Disease activity index was evaluated;colon changes were observed by HE staining;colon tissue damage index was evaluated;The expression of PI3K,AKT,PTEN,TLR2,Caspase-3,and Caspase-9 proteins was detected by Western Blotting in colon tissue.Results Compared with the blank group,the protein expression levels of PI3K,AKT,Caspase-3,Caspase-9 and TLR2 in the model group were increased(P<0.05),and the protein expression level of PTEN was decreased(P<0.05).Compared with the model group,the expression levels of PI3K,AKT,Caspase-3,Caspase-9 and TLR2 proteins in the Changyuning high-dose group were significantly decreased(P<0.05),and the protein expression levels of PTEN were significantly increased(P<0.05);The levels of PI3K,AKT and Caspase-3 in the salazine group and Changyuning medium dose were decreased(P<0.05),the expression level of PTEN protein was increased(P<0.05),and there was no statistical significance in the expression of Caspase-9;There was no statistical significance in the expression of each protein between the Changyuning low-dose group and the model group.Conclusion Changyuning recipe has a significant effect on the treatment of UC,and its mechanism may be related to the regulation of PI3K/AKT signaling pathway and its related proteins PTEN,TLR2,Caspase-3,and Caspase-9,which can effectively relieve the symptoms of UC rats and reduce colon pathological damage.

3.
Journal of Chinese Physician ; (12): 184-187, 2020.
Article in Chinese | WPRIM | ID: wpr-867220

ABSTRACT

Objective To investigate the relationship between perineural invasion (PNI) and the clinicopathological features of cervical cancer,and to explore its influence on the prognosis of cervical cancer patients.Methods Retrospective analysis the clinical data of 76 patients with early cervical cancer who received radical hysterectomy + pelvic lymphadenectomy ± paraaortic lymphadenectomy from November 2015 to December 2019 in Xuanwu Hospital.The clinicopathological characteristics of the postoperative pathological results of cervical cancer patients were analyzed to explore the related factors of PNI and its influence on prognosis.Results Single factor analysis suggested that the incidence of PNI was related to tumor diameter,depth of stroma invasion,lymph-vascular space invasion,lymph node metastasis and clinical stage (P < 0.05).Multivariate analysis suggested that the infiltration depth of stroma invasion was the risk factor of PNI (P < 0.05).K-M analysis showed that there was no significant difference in disease-free survival and overall survival between PNI positive and PNI negative patients (P > 0.05).Conclusions PNI is related to the depth of interstitial infiltration.PNI has no significant effect on the short-term recurrence rate and short-term survival rate of cervical cancer patients.

4.
Article in Chinese | WPRIM | ID: wpr-510107

ABSTRACT

TCM gel paste is a new transdermal medicine delivery system, with a lot of advantages, which has been widely used in clinic. By searching domestic research literature about TCM gel paste in recent years, this article reviewed from patent overview, matrix, transdermal absorption enhancer, preparation technology, quality evaluation, clinical efficacy and safety, problems and prospects, with a purpose to provide references for clinical medication of TCM gel paste.

5.
Chongqing Medicine ; (36): 658-660,664, 2017.
Article in Chinese | WPRIM | ID: wpr-606295

ABSTRACT

Objective To investigate the polymorphisms of the 19 Y-STR loci in the Han population in Northeast China,and analyze the genetic relationships of 15 ethnic groups in northeast Asia region,and to evaluate their forensic value and population genetic value.Methods The 19 Y-STR unrelated Han males in 200 unrelated Han males in Northeast China were amplified with the Y-filer system,and the PCR products were analyzed by the 310 Genetic Analyzer.The AMOVA analysis,cluster analysis and MDS analysis were calculated by Arelquin3.11,Mega4.1 and SPSS17.0.Results The polymorphisms of 19 Y-STR loci in the Han population in Northeast China had generally higher gene diversity value which were ranged from 0.350 9 (DYS391) to 0.971 1 (DYS385a/b),and totally 200 haplotypes were observed.The 19 Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,indicating that these 19 loci were useful genetic markers for forensic personal identification and paternity testing.There were distinctions among 15 ethnic groups.The genetic distance between 15 ethnic groups were ranged from 0.000 9 to 0.643 2,and the conclusion of cluster analysis and MDS analysis were similar to the ethnogeny research and ethnic migration history.Conclusion The 19 Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,and these 19 loci were useful genetic markers for forensic personal identification and paternity testing.

6.
Chongqing Medicine ; (36): 4086-4088,4091, 2015.
Article in Chinese | WPRIM | ID: wpr-602845

ABSTRACT

Objective To investigate the relationship between IL‐10 SNPs and breast cancer susceptibility .Methods Collect‐ed 156 blood samples of breast cancer patients as case group and 156 blood samples of health as control .Genotype SNPs of IL‐10‐592 ,‐1082 were analyzed by genomic DNA extraction ,PCR amplification and TOF mass spectrometry .The data was used by statis‐tical analysis .Results IL‐10‐592 C/C ,C/A and A/A site the genotype frequencies were not diversity between two groups(χ2 =3 .26 ,P>0 .05) .IL‐10‐1082 G/G、G/A、A/A site the genotype frequencies were statistically significant between two groups(χ2 =14 .07 ,P<0 .01) .In Logistic multivariate regression analysis found that :be compared with IL‐10‐592 A/A ,carrying IL‐10‐592 C/A ,C/C group respectively the risk of breast cancer were OR=1 .039(95% CI:0 .484 -2 .233) ,P=0 .922 ,OR= 1 .635(95% CI:0 .683-3 .915) ,P =0 .270 ,which had no statistical significance .In Logistic multivariate regression analysis found that :be com‐pared with IL‐10‐1082 A/A ,carrying IL‐10‐1082 G/A、G/G group respectively the risk of breast cancer were OR=0 .424(95% CI:0 .210-0 .855) ,P=0 .016 ,OR=0 .455(95% CI:0 .178 -1 .163) ,P= 0 .100 .Conclusion IL‐10‐592 may be not associated with breast cancer susceptibility .IL‐10‐1082 G/A may be a protection factor with breast cancer susceptibility .

7.
Article in Chinese | WPRIM | ID: wpr-491241

ABSTRACT

Objective To investigate the influence of hedysaryum polysaccharide (HPS)in the kidney function and expressions of Glut-1 mRNA and protein in kidney tissue of db/db mice with diabetic nephropathy (DN)and to elucidate its possible action mechanism.Methods 10 db/m mice were taken as normal control group(n=10);50 fueling animal model db/db mice with DN were randomly divided into model group,enalapril group and the low, middle and high doses of HPS groups(n=10).The mice in noral control group and model group were given physioloical saline by gavege;and the mice in the other groups were respectively given 10 mg·kg-1 ·d-1 enalapril, 100,200 and 400 mg·kg-1 ·d-1 HPS by gavage;lasted 8 weeks.Picric acid method was used to determine the serum creatinine(SCr)level of the mice,enzyme coupling rate method was used to determine the blood urea nitrogen (BUN)level,ELISA method was used to determine the urinary microalbumin(UMALB)level,RT-PCR method was performed to detect the expression of Glut-1 mRNA, and Western blotting and immunohistochemical methods were used to detect the expression of Glut-1 protein.Results Compared with model group,the levels of SCr, BUN, UMALB, the mRNA and protein of Glut-1 expressions were decreased, especially in 400 mg·kg-1 ·d-1 HPS and enalapril groups(P<0.01).The HE and Masson staining results showed that less inflammatory cells infiltration in glomerular of the mice were found, capillary lumens were unobstructed, and the collagen deposition was not obvious in 400 mg·kg-1 ·d-1 group.Conclusion HPS could improve the kidney function of the db/db mice and inhibit the Glut-1 mRNA and protein expressions obviously, which indicates that HPS could delay the development of DN by inhibiting the Glut-1 expression in the glomerular mesangial cell membrane.

8.
Article in Chinese | WPRIM | ID: wpr-563670

ABSTRACT

Objective To study the expression of Bcl-2 and E2F-1 in human adenomyosis and to explore the correlation between the expression and pathogenesis of human adenomyosis. Methods Immunohistochemical S-P method was employed to detect the expression of Bcl-2 and E2F-1 in 58 specimens of ectopic endometrium, eutopic endometrium of adenomyosis and 30 specimens of normal endometrium. Results The expression of Bcl-2 and E2F-1 in ectopic and eutopic endometrium of adenomyosis were significantly higher than those of normal endometrium (P

9.
Article in Chinese | WPRIM | ID: wpr-558494

ABSTRACT

Objective This study was designed to investigate the expression and clinical significance of transcription factor E2F-1 and apoptosis-off gene Bcl-2 in human colorectal carcinoma.Methods Immunohistochemistry is used to detect the expression of E2F-1、Bcl-2 proteins in 60 cases of human colorectal carcinoma tissues and their corresponding normal mucosa.Results The positive expression rate of E2F in cancer tissues is 56.7%,while the positive expression rate of E2F in corresponding normal mucosa is 6.7%,so the comparison between two tissues is significant (P

10.
Article in Chinese | WPRIM | ID: wpr-680535

ABSTRACT

Objective To study the effects of ganoderma applanatum polysaccharides(GAPS) on cell morphology, proliferation and PDGFR -?expression in cell lines MGC - 803 , and to explore its potential mechanisms of anti - tumor of GAPS. Methods Cell morphology was observed by inverting microscope. MTT assay was used to investigate the inhibitory effect of GAPS on MGC -803.Expressions of PDGFR -?was analyzed by flow cytometry (FCW). The fluorescence intensity of expressions of PDGFR -?was observed by fluorescence microscope. Results Cells of GAPS group were irregular shaped and grew poorly. GAPS inhibited the proliferation of MGC -803 cells in dose - dependent and time - dependent manners . After MGC - 803 cells were treated with GAPS for 72h, GAPS could down - regulate expression of PDGFR -?observed by fluorescence microscope which was consistented with the results of FCW with statistic significance difference as compared with control group (P

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