Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 58
Filter
Add filters








Year range
1.
Chinese Journal of Biotechnology ; (12): 673-679, 2021.
Article in Chinese | WPRIM | ID: wpr-878592

ABSTRACT

Nucleic acid detection technique has good sensitivity and specificity and is widely used in in vitro diagnosis, animal and plant commodity quarantine, forensic identification, and other fields. However, it is susceptible to carryover contamination during the operation and leads to false-positive results, which seriously affects the detection accuracy. Therefore, finding an effective solution to prevent and eliminate nucleic acid carryover contamination has become particularly urgent. This study compared several different methods for removing nucleic acid contamination and confirmed that sodium hypochlorite solution and PCRguard reagent could effectively eliminate nucleic acid carryover in the liquid and on surfaces of different materials. Besides, the combination of sodium hypochlorite solution and PCRguard can solve the nucleic acid aerosol contamination. This study proposes solutions for the routine prevention of carryover contamination and removal of aerosol that has occurred in molecular diagnostic laboratories.


Subject(s)
Laboratories , Nucleic Acids , Pathology, Molecular
2.
Cancer Research and Clinic ; (6): 66-69, 2020.
Article in Chinese | WPRIM | ID: wpr-872451

ABSTRACT

Under the regulation of various cytokines and different concentrations of cytokines, primary CD4 + T cells can differentiate into different Th subgroups. T helper cells 17 (Th17) and regulatory T cells (Treg cells) are research hotspots in recent years. They play diverse immunomodulatory roles by secreting various target cytokines. Th17 and Treg cells are different from each other but connect with each other, their regulatory mechanism is complex, and they play important roles in non-small cell lung cancer (NSCLC). This paper reviews the progress of the differentiation, the development and the immunological function of Th17 and Treg cells and their immunomodulatory effect on the NSCLC.

3.
Article in Chinese | WPRIM | ID: wpr-866628

ABSTRACT

Objective:To investigate the correlation between cyclooxygenase-2(COX-2), estrogen receptor (ER), progesterone receptor(PR) and the formation of endometrial polyps and the increased malignant rate of postmenopausal endometrial polyps.Methods:From January 2018 to January 2019, a total of 160 postmenopausal women treated in the People's Hospital of Changxing County were selected and divided into endometrial polyps group(83 cases) and oothectomy group(42 cases), normal menopause group(35 cases) according to different diseases.The levels of COX-2, ER, PR in the three groups were measured.The expression of COX-2 in mesenchymal cells and glandular epithelial cells, the expression of ER in mesenchymal cells and glandular epithelial cells, the expression of PR in mesenchymal cells and glandular epithelial cells in the three groups were compared, and the correlation between COX-2, ER, PR and increased malignant rate of endometrial polyps was analyzed.Results:The positive expression rates of COX-2 in mesenchymal cells and glandular epithelial cells in the endometrial polyps group(16.9%, 30.1%) were higher than those in the normal menopause group(0.0%, 11.4%) and the oothectomy group(4.8%, 7.1%)(χ 2=4.568, 5.806, all P<0.05 ). There were no statistically significant differences in the expression of ER in mesenchymal cells and glandular epithelial cells among the three groups(χ 2=1.333, 1.412, all P>0.05). The expression of PR in mesenchymal cells and glandular epithelial cells in the endometrial polyps group was lower than that in the normal menopause group and the oothectomy group(χ 2=4.890, 5.022, all P<0.05). COX-2 was positively correlated with the increase in malignant rate of endometrial polyps( r=4.335, P<0.05), PR was negatively correlated with an increase in the malignant rate of endometrial polyps( r=-4.256, P<0.05), and ER had no significant correlation with an increase in the malignant rate of endometrial polyps( r=1.203, P>0.05). Conclusion:COX-2 and PR are significantly related to the formation of endometrial polyps and the increase in malignant rate of postmenopausal endometrial polyps, but there is no significant expression of ER.Therefore, the detection of COX-2 and PR levels is beneficial to provide a certain scientific basis for clinical treatment.

4.
Cancer Research and Clinic ; (6): 66-69, 2020.
Article in Chinese | WPRIM | ID: wpr-799305

ABSTRACT

Under the regulation of various cytokines and different concentrations of cytokines, primary CD4+ T cells can differentiate into different Th subgroups. T helper cells 17 (Th17) and regulatory T cells (Treg cells) are research hotspots in recent years. They play diverse immunomodulatory roles by secreting various target cytokines. Th17 and Treg cells are different from each other but connect with each other, their regulatory mechanism is complex, and they play important roles in non-small cell lung cancer (NSCLC). This paper reviews the progress of the differentiation, the development and the immunological function of Th17 and Treg cells and their immunomodulatory effect on the NSCLC.

5.
Chinese Journal of Biotechnology ; (12): 593-604, 2020.
Article in Chinese | WPRIM | ID: wpr-827009

ABSTRACT

An epidemic of acute respiratory syndrome in humans, which appeared in Wuhan, China in December 2019, was caused by a novel coronavirus (SARS-CoV-2). This disease was named as "Coronavirus Disease 2019" (COVID-19). SARS-CoV-2 was first identified as an etiological pathogen of COVID-19, belonging to the species of severe acute respiratory syndrome-related coronaviruses (SARSr-CoV). The speed of both the geographical transmission and the sudden increase in numbers of cases is much faster than SARS and Middle East respiratory syndrome (MERS). COVID-19 is the first global pandemic caused by a coronavirus, which outbreaks in 211 countries/territories/areas. The vaccine against COVID-19, regarded as an effective prophylactic strategy for control and prevention, is being developed in about 90 institutions worldwide. The experiences and lessons encountered in the previous SARS and MERS vaccine research can be used for reference in the development of COVID-19 vaccine. The present paper hopes to provide some insights for COVID-19 vaccines researchers.


Subject(s)
Betacoronavirus , Allergy and Immunology , Biomedical Research , Coronavirus Infections , Epidemiology , Allergy and Immunology , Virology , Humans , Internationality , Middle East Respiratory Syndrome Coronavirus , Allergy and Immunology , Pandemics , Pneumonia, Viral , Epidemiology , Allergy and Immunology , Virology , SARS Virus , Allergy and Immunology , Severe Acute Respiratory Syndrome , Allergy and Immunology , Viral Vaccines , Allergy and Immunology
6.
Article in English | WPRIM | ID: wpr-826305

ABSTRACT

BACKGROUND@#Adjuvants used in inactivated vaccines often upregulate type 2 immunity, which is dominant in allergic diseases. We hypothesised that cumulative adjuvant exposure in infancy may influence the development of allergies later in life by changing the balance of type 1/type 2 immunity. We examined the relationship between immunisation with different vaccine types and later allergic disease development.@*METHODS@#We obtained information regarding vaccinations and allergic diseases through questionnaires that were used in The Japan Environment and Children's Study (JECS), which is a nationwide, multicentre, prospective birth cohort study that included 103,099 pregnant women and their children. We examined potential associations between the initial vaccination before 6 months of age and symptoms related to allergies at 12 months of age.@*RESULTS@#Our statistical analyses included 56,277 children. Physician-diagnosed asthma was associated with receiving three (aOR 1.395, 95% CI 1.028-1.893) or four to five different inactivated vaccines (aOR 1.544, 95% CI 1.149-2.075), compared with children who received only one inactivated vaccine. Similar results were found for two questionnaire-based symptoms, i.e. wheeze (aOR 1.238, 95% CI 1.094-1.401; three vaccines vs. a single vaccine) and eczema (aOR 1.144, 95% CI 1.007-1.299; four or five vaccines vs. a single vaccine).@*CONCLUSIONS@#Our results, which should be cautiously interpreted, suggest that the prevalence of asthma, wheeze and eczema among children at 12 months of age might be related to the amount of inactivated vaccine exposure before 6 months of age. Future work should assess if this association is due to cumulative adjuvant exposure. Despite this possible association, we strongly support the global vaccination strategy and recommend that immunisations continue.@*TRIAL REGISTRATION@#UMIN000030786 .


Subject(s)
Asthma , Epidemiology , Cohort Studies , Dermatitis, Atopic , Epidemiology , Female , Food Hypersensitivity , Epidemiology , Humans , Hypersensitivity , Epidemiology , Infant , Infant, Newborn , Japan , Male , Vaccines, Inactivated , Viral Vaccines
7.
Article | WPRIM | ID: wpr-833681

ABSTRACT

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.

8.
Chinese Journal of Biotechnology ; (12): 2206-2215, 2020.
Article in Chinese | WPRIM | ID: wpr-878479

ABSTRACT

Dengue virus (DENV) is the most widely transmitted arbovirus in the world. Due to the lack of diagnostic technology to quickly identify the virus serotypes in patients, severe dengue hemorrhagic fever cases caused by repeated infections remain high. To realize the rapid differential diagnosis of different serotypes of DENV infection by immunological methods, in this study, four DENV serotype NS1 proteins were expressed and purified in mammalian cells. Monoclonal antibodies (MAbs) against NS1 protein were obtained by hybridoma technology after immunizing BALB/c mice. Enzyme-linked immunosorbent assay, indirect immunofluorescence assay, dot blotting, and Western blotting were used to confirm the reactivity of MAbs to viral native NS1 and recombinant NS1 protein. These MAbs include not only the universal antibodies that recognize all DENV 1-4 serotype NS1, but also serotype-specific antibodies against DENV-1, DENV-2 and DENV-4. Double antibody sandwich ELISA was established based on these antibodies, which can be used to achieve rapid differential diagnosis of serotypes of DENV infection. Preparation of DENV serotype-specific MAbs and establishment of an ELISA technology for identifying DENV serotypes has laid the foundation for the rapid diagnosis of DENV clinical infection.


Subject(s)
Animals , Antibodies, Monoclonal , Antibodies, Viral/metabolism , Dengue/diagnosis , Dengue Virus/immunology , Enzyme-Linked Immunosorbent Assay , Humans , Mice , Mice, Inbred BALB C , Sensitivity and Specificity , Serogroup , Viral Nonstructural Proteins/immunology
9.
Chinese Journal of Dermatology ; (12): 607-610, 2019.
Article in Chinese | WPRIM | ID: wpr-797843

ABSTRACT

Objective@#To detect gene mutations in a pedigree with Rothmund-Thomson syndrome (RTS) .@*Methods@#Clinical data were collected from two patients (an older sister and a younger brother) and their family members in a Chinese pedigree of Han nationality with RTS. Blood samples were obtained from the two patients, their unaffected older brother, their parents and 100 unrelated healthy controls. DNA was extracted, and all the exons in the encoding area of the RECQL4 gene were amplified by PCR. Gene mutations were detected by a skin-targeted next-generation sequencing panel, and verified by Sanger sequencing.@*Results@#Two heterozygous mutations were identified in the RECQL4 gene of the two patients, including a splice site mutation c.2886-1G>A and an insertion mutation c.1013_1014insC, which were inherited from the father and mother of the patients respectively. Meanwhile, neither of the two mutations was observed in 100 unrelated healthy controls or the older brother of the patients.@*Conclusion@#The splice site mutation c.2886-1G>A and the insertion mutation c.1013_1014insC in the RECQL4 gene may contribute to the clinical phenotype of the patients in this pedigree with RTS.

10.
Article in Chinese | WPRIM | ID: wpr-804609

ABSTRACT

Objective@#To study the effects of Phellodendron amurense on herpes simplex virus 1 (HSV-1) and cytokines, and to explore the mechanism of Phellodendron amurense inhibiting HSV-1 virus through multiple channels.@*Methods@#Viruses were inoculated into medicine treated HeLa cells. The proliferation of virus was observed by fluorescence microscopy. The transcriptional levels of glycoprotein gD and functional protein US1 on the surface of virus envelope were detected by quantitative (q) PCR. After incubating HeLa cells for 24 h, qPCR was used to detect the expression of intrinsic immune factors such as IP-10, IL-12, IFN-gamma and transcription factor NF-kappa B (P65). The expression and nuclear location of NF-kappa B (P65) protein were detected by immunofluorescence.@*Results@#Fluorescence showed that the proliferation of virus decreased significantly at 8 and 40 ng/ml (P<0.01), and the transcription levels of viral protein gD and US1 decreased (P<0.05). After incubation for 24 hours, the transcription levels of IP-10, IL-12 and IFN-gamma in HeLa cells increased significantly (P<0.01). The transcription level of transcription factor NF-kappa B (P65) also increased (P<0.05), and immunofluorescence showed that the nuclear penetration rate of p65 subunit of NF-kappa B (P65) in each group increased (P<0.05).@*Conclusions@#Phellodendron amurense extract can inhibit HSV-1 by inhibiting the transcription of viral functional protein and promoting the expression of cellular immune factors.

11.
Chinese Journal of Dermatology ; (12): 607-610, 2019.
Article in Chinese | WPRIM | ID: wpr-755814

ABSTRACT

Objective To detect gene mutations in a pedigree with Rothmund-Thomson syndrome (RTS).Methods Clinical data were collected from two patients (an older sister and a younger brother)and their family members in a Chinese pedigree of Han nationality with RTS.Blood samples were obtained from the two patients,their unaffected older brother,their parents and 100 unrelated healthy controls.DNA was extracted,and all the exons in the encoding area of the RECQL4 gene were amplified by PCR.Gene mutations were detected by a skin-targeted next-generation sequencing panel,and verified by Sanger sequencing.Results Two heterozygous mutations were identified in the RECQL4 gene of the two patients,including a splice site mutation c.2886-1G>A and an insertion mutation c.1013_1014insC,which were inherited from the father and mother of the patients respectively.Meanwhile,neither of the two mutations was observed in 100 unrelated healthy controls or the older brother of the patients.Conclusion The splice site mutation c.2886-1G>A and the insertion mutation c.1013_1014insC in the RECQL4 gene may contribute to the clinical phenotype of the patients in this pedigree with RTS.

12.
Article in Chinese | WPRIM | ID: wpr-753579

ABSTRACT

Objective To study the effect of Zhibitai capsule on blood lipids,blood glucose and omentin in hyperlipidemic rats.Methods Thirty SPF male rats were randomly divided into control group, model group and Zhibitai group.The control group was fed with common diet,while the rest rats were fed with high-fat diet.After the model was successfully established,the Zhibitai group was orally administered 8mg/kg Zhibitai capsule powder twice daily,the rats in the control group and the model group were given the same amount of normal saline respectively. After 4 weeks,the serum levels of total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL-C),low density lipoprotein (LDL-C),blood glucose and omentin were evaluated.Results Compared with the model group, the serum levels of TC[(3.97 ± 0.62) mmol/L vs.(6.33 ± 1.10) mmol/L,t =5.910,P <0.01],TG[(1.51 ± 0.47)mmol/L vs.(2.18 ± 0.64)mmol/L,t=2.472,P<0.05]and LDL-C[(0.30 ± 0.04)mmol/L vs.(0.48 ± 0.16) mmol/L, t =3.451, P <0.01)] of the Zhibitai group were significantly decreased,the HDL -C level was significantly increased[(1.35 ± 0.28) mmol/L vs.(1.06 ± 0.31) mmol/L,t =2.195,P <0.05],blood glucose decreased significantly[(5.04 ± 0.82)mmol/L vs.(6.43 ± 1.10)mmol/L,t=3.204,P<0.01],and the concentration of omentin was significantly increased[(68.59 ± 24.82) ng/L vs.(46.86 ± 20.12) ng/L,t =2.151,P <0.05]. Conclusion Zhibitai capsule can effectively reduce the serum levels of TC,TG and LDL-C of hyperlipidemic rats and increase the content of HDL-C,effectively reduce blood sugar,and can increase the content of omentin.

13.
Article in Chinese | WPRIM | ID: wpr-744436

ABSTRACT

Objective To study the effect of Zhibitai capsule on blood lipids,blood glucose and omentin in hyperlipidemic rats. Methods Thirty SPF male rats were randomly divided into control group,model group and Zhibitai group. The control group was fed with common diet,while the rest rats were fed with high - fat diet. After the model was successfully established,the Zhibitai group was orally administered 8mg/ kg Zhibitai capsule powder twice daily,the rats in the control group and the model group were given the same amount of normal saline respectively. After 4 weeks,the serum levels of total cholesterol (TC),triglyceride (TG),high density lipoprotein (HDL - C),low density lipoprotein (LDL - C),blood glucose and omentin were evaluated. Results Compared with the model group, the serum levels of TC[(3. 97 ± 0. 62) mmol/ L vs. (6. 33 ± 1. 10) mmol/ L,t = 5. 910,P < 0. 01],TG[(1. 51 ± 0. 47)mmol/ L vs. (2. 18 ± 0. 64)mmol/ L,t = 2. 472,P < 0. 05]and LDL - C[(0. 30 ± 0. 04)mmol/ L vs. (0. 48 ± 0. 16)mmol/ L,t = 3. 451,P < 0. 01)] of the Zhibitai group were significantly decreased,the HDL - C level was significantly increased[(1. 35 ± 0. 28) mmol/ L vs. (1. 06 ± 0. 31) mmol/ L,t = 2. 195,P < 0. 05],blood glucose decreased significantly[(5. 04 ±0. 82)mmol/ L vs. (6. 43 ± 1. 10)mmol/ L,t = 3. 204,P < 0. 01],and the concentration of omentin was significantly increased[(68. 59 ± 24. 82) ng/ L vs. (46. 86 ± 20. 12) ng/ L,t = 2. 151,P < 0. 05]. Conclusion Zhibitai capsule can effectively reduce the serum levels of TC,TG and LDL - C of hyperlipidemic rats and increase the content of HDL - C,effectively reduce blood sugar,and can increase the content of omentin.

14.
Chinese Journal of Biotechnology ; (12): 2025-2034, 2018.
Article in Chinese | WPRIM | ID: wpr-771406

ABSTRACT

Ebola virus (EBOV) is an extremely contagious pathogen first discovered in Africa associated with severe hemorrhagic disease in humans and nonhuman primates, which has resulted in at least 28 500 suspected cases and 11 300 confirmed deaths in 2014-2016 Ebola epidemic in West Africa. Rapid and sensitive detection of EBOV is the key to increasing the probability of survival and reducing infection rates in pandemic regions. Here, we report an ultrasensitive and instrument-free EBOV detection assay based on colloidal carbon immunochromatography. Carbon nanoparticle-labeled rabbit anti-EBOV-VP40 IgG were concentrated in the conjugate pad, monoclonal antibody (McAb, 4B7F9) against EBOV-VP40 and goat anti-rabbit IgG were immobilized on the nitrocellulose membrane with 2 μL/cm at a concentration of 1 mg/mL as test and control lines, respectively. Then the sample application pad, conjugate release pad, nitrocellulose membrane and absorbent pad were assembled into a lateral flow test strip. The test strip shows strong specificity against related viruses that share similar clinical symptoms and geographic range with EBOV, including marburg virus, influenza virus, yellow fever virus and dengue virus. In addition, 1 500 negative serums were tested with false-positive rate of 1.3‰ which significantly lower than that of ReEBOV™ colloidal gold test kit recommended by World Health Organization (WHO). The sensitivity of this strip was analyzed using inactivated EBOV with detection limit of 100 ng/mL (10⁶ copies/mL) which clearly higher than that of ReEBOV™ dipstick (10⁸ copies/mL). Furthermore, the strip showed excellent thermal stability characteristics in room temperature and could be as a point-of-care (POC), ultra-sensitive and specific promising candidate for EBOV serological screening in rural Africa or entry/exit ports.


Subject(s)
Animals , Carbon , Ebolavirus , Hemorrhagic Fever, Ebola , Humans , Nanoparticles , Rabbits
15.
Article in Chinese | WPRIM | ID: wpr-513223

ABSTRACT

Objective To investigate the value of radionuclide bone imaging in the diagnosis and localization of osteoporotic vertebral compressive fracture.Methods Percutaneous vertebroplasty was applied to 40 patients suffering from osteoporotic vertebral compressive fracture.Responsible vertebral bodys located according to the radionuclide bone imaging was involved as bone scan group,and responsible vertebral bodys located according to the physical examination,X-ray,CT and MRI as standard group.Then the Kappa test was carried out.Result In bone scan group,56 vertebrae were located by radionuclide bone imaging,and in standard group,58 vertebrae were located by X-ray,54 vertebrae by CT and 52 vertebrae by MRI.The Kappa value was 0.76,indicating essentially excellent agreement and statistical significance.Conclusion Radionuclide bone imaging was another good choice for the diagnosis of osteoporotic vertebral compressive fracture.Especially for cases with contraindication of MRI,it offers a reliable choice.

16.
Chinese Journal of Geriatrics ; (12): 872-876, 2017.
Article in Chinese | WPRIM | ID: wpr-611144

ABSTRACT

Objective To survey a prevalence of osteoporosis and prevalence of osteoporosis combined with hyponatremia in elderly hospitalized patients,and their risk factors.Methods We enrolled 2496 elderly hospitalized patients with detected plasma levels of sodium,calcium,25 (OH) D3,PTH,plasma PINP,and β-CTX.At the same time,sex,age,height,weight,smoking history,drinking history and BMI(kg/m2) in form of a questionnaire were recorded and calculated.The risk factors for osteoporosis were analyzed using multivariate Logistic regression method.Results The osteoporosis prevalence was 12.2% (305/2496 inpatients)with 31.5 % (96/305)in male,68.5% (209/305)in female(x2 =4.651,P=0.031).The prevalence of osteoporosis with hyponatremia was 27.5 % (84/305),with 24.8 % (21/84) in male and 75.2 % (63/84) in female(x2 =9.251,P=0.025).As compared with three groups of non-osteoporosis,normal serum Na+ with and without osteoporosis,the osteoporosis patients with hyponatremia were more aged,in a higher proportion of women and smokers,in lower BMI,and in low levels of serum sodium,BMD 25(OH)D3 (F=13.783,0.861,7.146,24.520,0.548,x2 =15.113、4.472;P =0.001,0.000,0.021,0.015,0.003,0.021,0.005).Multivariate Logistic regression analysis showed that aging,female,low BMI,smoking history,drinking history,low plasma 25(OH)D3 level,low plasma PINP level,and high plasma β-CTX level were the risk factors for osteoporosis(OR 4.215,2.271,3.176,2.013,1.237,3.987,1.843,1.972;all P<0.05).Conclusions The osteoporosis prevalence is high in elderly patients,especially in old women.The risk factors for osteoporosis are diverse,and clinical conditions of osteoporosis patients with hyponatremia are much more severe than the others.More efforts should be given to them and need to be focused on the complications of osteoporosis.

17.
Article in Chinese | WPRIM | ID: wpr-614999

ABSTRACT

BACKGROUND:Percutaneous vertebroplasty is mainly used to treat senile osteoporotic compressive fractures,while for young patients,pedicle screw internal fixation is usually applied,but heavy trauma,nail broken,secondary surgery and other problems can be found occasionally.OBJECTIVE:To investigate the clinical efficacy of percutaneous vertebroplasty combined with granulated allogeneic bone grafting for thoracolumbar compressive fractures.METHODS:Twenty-one patients suffering thoracolumbar compressive fractures were recruited,and underwent percutaneous vertebroplasty combined with granulated allogeneic bone grafting.The Visual Analogue Scale scores,height ratio of fractured vertebrae and Cobb angle were detected before and after surgery.RESULTS AND CONCLUSION:(1) Compared with baseline,the Visual Analogue Scale scores were improved significantly at 1 day and 6 months postoperatively (P < 0.05).(2) The height ratio of fractured vertebrae at 1 day and 6 months postoperatively was significantly higher than that before treatment (P < 0.05).(3) The Cobb angle of fractured vertebrae at 1 day and 6 months postoperatively was significantly less than that before treatment (P < 0.05).(4) The mean operation time was (52±12.3) minutes,and the mean blood loss was (11.5±1.5) mL.(5) These results suggest that percutaneous vertebroplasty combined with granulated allogeneic bone grafting can effectively restore the anterior fractured vertebral height,eliminate the cavity in the fractured vertebrae,renew the completeness and stabilization of the fractured vertebrae,which keep the spinal range of motion;therefore,it is a minimal invasive method in the treatment of thoracolumbar fractures.

18.
Article in Chinese | WPRIM | ID: wpr-514103

ABSTRACT

Objective To explore the expression and significance of galectin-3 in COPD and lung cancer.Methods Sixty female SD rats were randomly divided into three groups:COPD group,lung cancer group and the control group with 20 rats in each group.COPD group was given tracheal injection of lipopolysaccharide and exposed to daily cigarette to establish the model.Lung cancer group was injected with 3,4-benzopyrene-corn mixture.The control group was fed normally.After 20 weeks,plasma galectin-3 concentration was assessed in each group using ELISA method.Results The galectin-3 average concentrations of COPD group,lung cancer group and the control group were (8.63 ± 0.46) ng/mL,(9.15 ± 0.69) ng/mL and (4.47 ± 0.51) ng/mL.The average concentrations in COPD and lung cancer groups were higher than that of the control group with a statistically significant difference (P<0.05).Conclusion As expressed in COPD and lung cancer,galectin-3 may own a great significance to the occurrence,development and prognosis of COPD and lung cancer.

19.
Chinese Journal of Geriatrics ; (12): 572-576, 2016.
Article in Chinese | WPRIM | ID: wpr-496634

ABSTRACT

Objective To investigate the sodium and potassium disturbances in 9870 elderly inpatients and to analyze their risk factors.Methods Clinical data of sodium and potassium levels and the possible risk factors were collected in the elderly inpatients from a single center.The incidence of sodium and potassium disturbances and their risk factors were analyzed by multiple Logistic regression,and the correction of the imbalance was investigated.Results A total of 6027(61.6%)times of sodium and potassium disturbances were found in the 9870 elderly inpatients on admission and during hospitalization,and the total incidence of this disturbances was 61.6%.The incidences of hyponatremia,hypokalemia,hypernatremia and hyperkalemia were 27.9% (2729/6027),9.7% (951/6027),15.4%(1506/6027) and 8.6% (841/6027),respectively.Heart failure was the common risk factor for the two electrolyte disturbances.T2DM caused hyponatremia,hypokalemia and hypernatremia.Among the medication risk factors,diuretics were the common risk factor for various electrolyte disturbances.Patients taking antiepileptics,antidementia drugs,antidepressants and benzodiazepines were more likely to have hyponatremia.The correction rate of mild,moderate and severe hyponatremia/ hypokalemia were 79.2%(1253/1582),68.1% (535/786),45.1% (163/361) and 79.5%(776/976),66.4%(217/327),40.1%(81/203),respectively.The time for the correction of the above degrees of hyponatremia/ hypokalemia were(3.7±2.7) d,(4.1±2.3) d,(8.9±1.6) d and(2.5± 1.4) d,(3.2 ± 1.5) d,(6.1 ± 1.2) d.The supplement amounts of sodium chloride and potassium chloride were(5.98±3.67) g,(9.45±3.02) g,(10.26±1.32) g and(2.23±0.93) g,(5.12± 1.53) g,(8.07 ± 2.46) g,respectively.Conclusions The incidences of electrolyte disturbances are high in elderly inpatients with combined diseases and application of various drugs,and the correction of disturbances is difficult.More attention should be paid to the electrolyte disturbances,which should be corrected positively.

20.
China Journal of Endoscopy ; (12): 94-96, 2016.
Article in Chinese | WPRIM | ID: wpr-621273

ABSTRACT

Objective To explore and analyze the application and problems of esophageal cancer radical opera-tion by thoracoscopy combined with laparoscopy. Methods From July, 2011 to December, 2014, clinical data of 40 patients undergoing esophageal cancer radical operation by thoracoscopy combined with laparoscopy were analyzed retrospectively. Results Except 2 cases being relayed to assisted small thoracic incision because of 1 case with clear tumor invading to trachea and 1 case with obvious tumor invasion to thoracic aortic artery, the remaining 38 cases were successfully completed operation. There were 2 cases with postoperative anastomotic fistula in the neck, 2 cases of anastomotic stenosis and 1 case of hoarseness, there was no perioperative death. Follow-up was within 36 months, 2 patients died 10 months and 24 months after surgery respectively, due to tumor local recurrence. 1 patient with supraclavicular lymph node metastasis underwent local radiation therapy. The remaining was all survival with re-lapse-free. Conclusions Esophageal cancer radical operation by thoracoscopy combined with laparoscopy is a good method with safety and reliability, minimal invasion, fast recovering, and satisfied recent curative effect. It is worthy of applying clinically.

SELECTION OF CITATIONS
SEARCH DETAIL