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1.
Article in Chinese | WPRIM | ID: wpr-910052

ABSTRACT

Objective:To evaluate the efficacy and safety of perioperative rehabilitation approaches based on the concept of Enhanced Recovery After Surgery (ERAS) for pelvic fractures.Methods:A prospective randomized control trial was conducted to include 114 emergency patients who had been admitted to Department of Orthopaedic Trauma, Beijing Jishuitan Hospital for surgical treatment of pelvic fractures from June 2019 to December 2020. Of them, 57 were assigned into an intervention group according to a random digits table. They were 42 males and 15 females, aged from 18 to 77 years and subjected to management of pelvic fractures with tentative perioperative ERAS approaches which were adjusted at different stages. The other random 57 patients were assigned into a control group. They were 40 males and 17 females, aged from 17 to 70 years and subjected to management of pelvic fractures with conventional rehabilitation approaches which included postoperative in-hospital consultation and guidance by rehabilitation physicians. The 2 groups were compared in terms of Majeed pelvis scores and Barthel indexes at postoperative 2, 6, 12 and 24 weeks, and visual analogue scale (VAS) pain scores and SF36 scores at postoperative 12 and 24 weeks.Results:A total of 105 patients (55 in the intervention group and 50 in the control group) were completely followed up for 151 to 254 d (mean, 177 d). The 2 groups were comparable due to no significant difference between them in the preoperative general data ( P>0.05). The Majeed scores (44±13, 67±16, 86±14 and 98±7) and Barthel indexes (57±13, 79±16, 95±8 and 100±2) at postoperative 2, 6, 12 and 24 weeks in the intervention group were significantly higher than those in the control group [(35±16, 51±16, 73±14 and 91±12) and (45±19, 67±18, 86±12 and 98±4)] (all P<0.05). At postoperative 12 and 24 weeks, the SF-36 scores (129±15 and 141±6) in the intervention group were significantly higher than those in the control group (114±15 and 131±12) ( P<0.05). There was no significant difference in the pain degree between the 2 groups ( P>0.05). Conclusion:In management of pelvic fractures, compared with conventional perioperative rehabilitation approaches, the perioperative ERAS rehabilitation approaches may improve early functional outcomes and thus help the patients restore their activities of daily living earlier.

2.
Article in Chinese | WPRIM | ID: wpr-908769

ABSTRACT

Cordycepin,which has great immunomodulatory activities such as anticancer,antifungal,antivirus,antileukemia and lipid-lowering ones,is the secondary metabolite of Cordyceps militaris (C.militaris).Liquid submerged fermentation is the common cultivation process to produce cordycepin.To optimize the fermentation process and improve production,monitoring the cordycepin secretion in the fermen-tation is essential.The measurement based on chromatography-mass spectrometry methods is generally involved in the complex sample pretreatments and time-consuming separation,so more rapid and convenient methods are required.Matrix-assisted laser desorption ionization mass spectrometry(MALDI-MS) is more attractive for faster and direct detection.Therefore,MALDI-MS detection combined with isotope-labeled internal standard was applied to the measurement of cordycepin content in the fermentation broth and mycelium.This method made accurate quantification of cordycepin in the range of 5-400 μg/mL with a relative standard deviation of 5.6%.The recovery rates of fermentation samples after the 1,13,and 25 days were 90.15%,94.27%,and 95.06%,respectively.The contents of cordycepin in the mycelium and fermentation broth were 136 mg/g and 148.39 mg/mL on the 20th culture day,respectively.The cordycepin secretion curve of the liquid fermentation of C.militaris was real-time traced over 25 days.

3.
Article in Chinese | WPRIM | ID: wpr-908170

ABSTRACT

Objective:To apply the best evidence of evidence-based volume management in patients with chronic heart failure to clinical practice and evaluate its effect.Methods:From February 2018 to May 2019, according to the clinical evidence practice application mode of Joanna Briggs Institute (JBI), and the best evidence obtained in the previous study, we constructed audit indicators. And then perfomed clinical audits for the patients with chronic heart failure in the department of cardiology of Shandong Provincial Hospital affiliated to Shandong First Medical University. The obstacle factors were analyzed, and the action strategies were formulated. The cognition of the medical staff on the volume management, and the fasting body mass monitoring rate, the volume management target compliance rate, the time to dry body mass, average hospitalization day of the patients′, number of negative events related to volume management were compared before and after the application of the best evidence.Results:After the implementation of the reform, Indicator 8-14 and Indicator 20 of the 20 audit indicators implementation rate had been improved, and the difference was statistically significant ( χ 2 values were 6.668-97.000, P<0.05); the volume management target compliance rate increased from (34.24±30.33)% to (61.28±16.10)%, the difference was statistically significant ( t value was 30.21, P<0.001); the time to dry body mass decreased from (10.89±3.46) days to (5.48±1.58) days, the difference was statistically significant ( t value was 13.97, P<0.001); compared with the negative events related to the access control, the number of acute left heart failure cases decreased from 7 to 2 ( χ2 value was 4.194, P=0.041). Conclusion:Evidence-based volume management of patients with chronic heart failure can improve clinical nursing practice, shorten the time for patients to reach dry body mass, and reduce the average hospitalization day. Quality review still needs to continue to improve the quality of nursing.

4.
Article in Chinese | WPRIM | ID: wpr-921630

ABSTRACT

In this study, liquid chromatography-mass spectrometry(LC-MS) and high performance liquid chromatography(HPLC) were employed for qualitative and quantitative analysis of the steroidal saponins in rhizomes of Paris polyphylla var. yunnanensis from three different habitats cultured in vitro, in an attempt to explore whether the rhizomes of the medicinal herb cultured in vitro can synthesize the steroidal saponins, including polyphyllinsⅠ, Ⅱ, and Ⅶ, the quality markers specified in Chinese Pharmacopoeia(2020 edition). A total of 20 steroidal saponins were identified in the rhizomes from Changxin, Yunlong(S1), Fengyi, Dali(S2), and Niujie, Eryuan(S3): parisyunnanoside A and parisyunnanoside D or E, proto-polyphyllin Ⅱ, polyphyllins G and H, polyphyllinsⅠ, Ⅱ, Ⅴ, Ⅵ, and Ⅶ, dioscin, gracillin, prosapogenin A, Tg, isomer of Th, saponin Th, reclinatoside, proto-pairs D, pseudoproto-dioscin, and 23-O-glc-(23S,25R)-spirost-5-en-3β,23α,27-triol-3-O-rha-(1→2)-[ara(1→4)]-glc or 27-O-glc-(23S,25R)-spirost-5-en-3β,27α-diol-3-O-rha-(1→2)-[ara(1→4)]-glc. Among them, polyphyllinsⅠ, Ⅱ, and Ⅶ were detected in the rhizomes from S1, with the mass fraction of 0.109 1%, 0.165 2%, and 0.051 03%, respectively(total 0.325 3%). Polyphyllins Ⅱ and Ⅶ were identified in the rhizomes from S2 with the respective mass fraction of 0.192 2% and 0.074 23% and total content of 0.266 5%. Moreover, polyphyllins Ⅱ and Ⅶ were also found in the rhizomes from S3, which had the mass fraction of 0.207 7% and 0.186 9%, separately, with the total content of 0.394 6%. Thus, steroidal saponins, including the quality makers polyphyllins Ⅰ, Ⅱ, and Ⅶ recorded in Chinese Pharmacopoeia(2020 edition) can be synthesized in rhizomes of Paris polyphylla var. yunnanensis cultured in vitro, but their total content fails to meet the standard(0.60% in Chinese Pharmacopoeia). Therefore, in vitro culture of the Paris polyphylla var. yunnanensis is feasible, but the culture conditions need to be further improved.


Subject(s)
Chromatography, High Pressure Liquid , Liliaceae , Melanthiaceae , Rhizome , Saponins
5.
Chinese Medical Journal ; (24): 2685-2691, 2021.
Article in English | WPRIM | ID: wpr-921223

ABSTRACT

BACKGROUND@#Nitinol-containing devices are widely used in clinical practice. However, there are concerns about nickel release after nitinol-containing device implantation. This study aimed to compare the efficacy and safety of a parylene-coated occluder vs. a traditional nitinol-containing device for atrial septal defect (ASD).@*METHODS@#One-hundred-and-eight patients with ASD were prospectively enrolled and randomly assigned to either the trial group to receive a parylene-coated occluder (n = 54) or the control group to receive a traditional occluder (n = 54). The plugging success rate at 6 months after device implantation and the pre- and post-implantation serum nickel levels were compared between the two groups. A non-inferiority design was used to prove that the therapeutic effect of the parylene-coated device was non-inferior to that of the traditional device. The Cochran-Mantel-Haenszel chi-squared test with adjustment for central effects was used for the comparison between groups.@*RESULTS@#At 6 months after implantation, successful ASD closure was achieved in 52 of 53 patients (98.11%) in both the trial and control groups (95% confidence interval (CI): [-4.90, 5.16]) based on per-protocol set analysis. The absolute value of the lower limit of the 95% CI was 4.90%, which was less than the specified non-inferiority margin of 8%. No deaths or severe complications occurred during 6 months of follow-up. The serum nickel levels were significantly increased at 2 weeks and reached the maximum value at 1 month after implantation in the control group (P  0.05).@*CONCLUSIONS@#The efficacy of a parylene-coated ASD occluder is non-inferior to that of a traditional uncoated ASD occluder. The parylene-coated occluder prevents nickel release after device implantation and may be an alternative for ASD, especially in patients with a nickel allergy.


Subject(s)
Cardiac Catheterization , Heart Septal Defects, Atrial/surgery , Humans , Polymers , Prospective Studies , Prosthesis Design , Septal Occluder Device/adverse effects , Treatment Outcome , Xylenes
6.
International Eye Science ; (12): 1490-1493, 2021.
Article in Chinese | WPRIM | ID: wpr-882120

ABSTRACT

@#AIM:To evaluate the mitomycin-C(MMC)0.02% efficacy in preventing haze after trans-epithelial photorefractive keratectomy(Trans-PRK)in the treatment of the high myopia. <p>METHODS: Retrospective case series. Trans-PRK were performed on 142 eyes with a preoperative spherical equivalent. They were divided into with 0.02% MMC(MMC group)and without MMC(control)groups. In MMC group there were 94 eyes with MMC 0.02%; in control group there were 48 eyes. Patients were treated with an intraoperative application of MMC 0.02% for 30-45s depending on refractive error in MMC group. After surgery, fluorometholone 0.1% eye drops were used for 4mo in all groups. The mean follow-up time was 6mo. The regression trees were used to analyse the relationship between different related factors and haze. <p>RESULTS:Haze was quantified with Fantes. Incidence of haze was 8.5% eyes in MMC group and 33.3% in without MMC group(<i>P</i>=0.001). In the regression trees, optical zone and ablation depth MAX were related to haze(<i>P</i><0.01). It was possibility induce haze when optical zone is ≤5.6mm. When optical zone is >5.6mm, ablation depth MAX becomes the main factor for haze.<p>CONCLUSION: The design of optical zone and ablation depth MAX in Trans-PRK should be considered for the treatment of the high myopia with thin cornea and abnormal corneal morphology. MMC 0.02% was effective in preventing haze after Trans-PRK in the treatment of the high myopia.

7.
Chinese Medical Journal ; (24): 1329-1334, 2021.
Article in English | WPRIM | ID: wpr-878096

ABSTRACT

BACKGROUND@#With current chemotherapy treatment, >90% of survival has been obtained for Burkitt lymphoma (BL). In this study, the demographic characteristics and treatment outcomes are presented for 78 children in China with central nervous system-positive (CNS+) BL.@*METHODS@#This retrospective study consecutively enrolled 78 CNS+ BL patients in Beijing Children's Hospital (BCH) from 2007 to 2019 who received the BCH B-cell non-Hodgkin's lymphoma regimen (modified by French-American-British mature lymphoma B-cell 96 [FAB/LMB96] C1 arm ± rituximab). Clinical characteristics, methods of disease detection in the CNS, and outcomes were evaluated. Univariate and multivariate analyses were used to assess prognostic factors.@*RESULTS@#The median age of 65 boys and 13 girls at the time of diagnosis was 5.7 years (ranging from 1 to 14 years). Patients were followed up for a median time of 34 months (ranging from 1 to 72 months). Bone marrow invasion was found in 38 (48.7%) patients. There were 48 (61.5%), 44 (56.4%), and 25 (32%) patients with cranial nerve palsy, intracerebral mass (ICM), and para-meningeal extension, respectively. Abnormal cerebrospinal fluid (CSF) morphology and CSF immunophenotype appeared in 15 (19.2%) and 15 (19.2%) patients, respectively. There were 69 (88.5%) patients treated with chemotherapy combined with rituximab, and nine patients were treated solely with chemotherapy. Finally, five patients died of treatment-related infection, recurrence occurred for 13, and one developed a second tumor. The 3-year overall survival and event-free survival rates were 78.9% ± 4.7% and 71.4% ± 6.0%, respectively. Treatment with chemotherapy only, ICM positivity, and >4 organs involved at diagnosis were independent risk factors.@*CONCLUSIONS@#Rituximab combined with a modified LMB96 regimen has greatly increased the efficacy of treatment for Chinese children with CNS+ BL, and with the continuous collection of outcome data, treatment-related complications are decreasing. For further verification, a large sample multicentre randomized controlled study should be performed to explore a treatment scheme for Chinese children with even greater efficacy.


Subject(s)
Adolescent , Antineoplastic Combined Chemotherapy Protocols/therapeutic use , Burkitt Lymphoma/drug therapy , Central Nervous System , Child , Child, Preschool , China , Cyclophosphamide/therapeutic use , Disease-Free Survival , Female , Humans , Infant , Male , Neoplasm Recurrence, Local , Retrospective Studies , Rituximab/therapeutic use , Treatment Outcome
8.
Article in Chinese | WPRIM | ID: wpr-821651

ABSTRACT

Objective To investigate the risk of Anisakis infections among high-risk populations along the coastal areas of Jiangsu Province, so as to develop the strategy for the prevention and control of anisakiasis in the province. Methods Three counties along the coastal areas of Jiangsu Province were selected as the study sites in 2018, including Rudong County in Nantong City, Haizhou District in Lianyungang City and Dongtai City in Yancheng City. The knowledge, attitude and practice (KAP) of anisakiasis prevention and control, and the prevalence of serum specific IgG antibody against Anisakis were investigated among high-risk populations among these three study sites, including fishermen, fish seller and people who liked eating fresh and live marine fish. Factors affecting the prevalence of the specific IgG antibody against Anisakis were identified using a multiple logistic regression model. In addition, Anisakis larvae infections were detected in fresh and live marine fish samples collected from local markets, and the prevalence and intensity of Anisakis infections were estimated. Results A total of 625 high-risk populations were investigated, including 349 men (55.8%). Only 13.0% of the subjects heard about anisakiasis, and a low awareness rate of anisakiasis prevention and control knowledge was seen among these three types of high-risk populations. There were 21.6% of the subjects eating raw or half-cooked marine fish, 5.8% eating undercooked marine fish, 3.2% presenting vomiting, nausea and diarrhea after eating marine fish, 5.1% developing systemic allergic symptoms, and 65.6% using the same chopping board for raw and cooked food. The sero-prevalence of the anti-Anisakis IgG antibody was 7.0% among the study subjects. Multiple logistic regression analysis identified education level [OR = 0.687, 95% CI (0.478, 0.987)] and development of systemic allergic symptoms [OR = 4.641, 95% CI(1.411, 15.268)]as factors affecting the positive anti-Anisakis IgG antibody among the study subjects. Among 494 fresh and live marine fish detected, the prevalence and intensity of Anisakis larvae infection was 64.0% and 8.1 larvae per fish, with high prevalence seen in Trichiurus haumela and Pneumatophorus japonicas. Conclusions The awareness of anisakiasis prevention and control knowledge is low among the high-risk populations living along the coastal areas of Jiangsu Province, and there are high-risk behaviors, such as eating raw or half-cooked food, using the same chopping board for raw and cooked food. In addition, the prevalence of Anisakis infections is high in the marine fish in these areas. Therefore, the health education and health promotion for anisakiasis prevention and control should be intensified.

9.
Article in Chinese | WPRIM | ID: wpr-829568

ABSTRACT

Soil-transmitted nematodiasis was once widely prevalent in Jiangsu Province, which seriously threatened human health and hindered socioeconomic development. The control efforts over decades resulted in a remarkable decline in the prevalence of soil-transmitted nematode human infections in Jiangsu Province, with a reduction from 59.32% in 1989 to 0.12% in 2019, and the human prevalence remains at < 0.5% since 2013. Since 1987, an integrated strategy has been adopted for the control of soil-transmitted nematodiasis in Jiangsu Province; however, the core interventions varies at different stages, which mainly include deworming, water and sanitation service improvement, health education, and monitoring and assessment. The criteria of effective soil-transmitted nematodiasis control had been achieved in all epidemic counties (districts) of Jiangsu Province by 2019. Further actions to strengthen health education and monitoring and implement precision control measures are required to consolidate the achievements of soil-transmitted nematodiasis control and eliminate the harm of soil-transmitted nematodiasis to humans. This review summarizes the epidemiology, control progress and evolution of control strategy of soil-transmitted nematodiasis in Jiangsu Province.

10.
Chinese Journal of Orthopaedics ; (12): 781-788, 2019.
Article in Chinese | WPRIM | ID: wpr-755218

ABSTRACT

Objective To explore the clinical outcome an anatomic quadrilateral surface plate for both?column acetabu?lar fractures through the Stoppa combined with iliac fossa approach. Methods A retrospective study of the patients with both?col?umn acetabular fractures treated with an anatomic quadrilateral surface plate through the Stoppa combined with iliac fossa ap?proach from November 2015 to June 2017 was performed. Nineteen patients including 16 males and 3 females met the inclusion criteria. The mean age of included patients was 49.11 years (range, 25-68 years). There were 8 cases of fall injury, 7 cases of car accident injury and 4 cases of crushing injury. There were 11 cases of C1 type injury, 7 cases of C2 type, and 1 case of C3 type ac?cording to Tile classification. Patients included in this study were both?column acetabular fractures according to Letournel?Judet classification. There were six cases associated with posterior wall detachment. Minimally Stoppa combined with iliac fossa ap?proach was performed to manage the displaced fragments. First, the anteromedially displaced iliopubic fragment was managed with a reconstruction plate through iliac fossa approach. Then, the medially displaced ischiadic fragment could be fixed with an anatom?ic quadrilateral surface plate through the Stoppa approach. The reduction quality was assessed by postoperative radiographs and CT scans according to the criteria proposed by Matta. The function of hip joint was assessed by the Merle d’Aubigné?Posteal score modified by Matta. Results The average operation time and blood loss were 115.26 min (range, 90-160 min) and 534.21 ml (range, 300-1000 ml), respectively. The mean length surgical incision was 18.82 cm(range, 16-20 cm). Average follow?up time was 19.84 months (range, 12-28 months). According to Matta criteria of reduction quality, anatomic reduction was obtained in 14 cases, and satisfactory reduction was gained in 3 cases, while unsatisfactory reduction was found in 2 cases (satisfactory rate= 89.47%). All fractures healed well and mean healing time was 3.32 months (range, 3-5 months). The mean Merle d’Aubigné?Post?eal score modified by Matta was 16.95 (range, 13-18), including 12 cases of excellent, 4 cases of good, and 3 cases of fair (satisfac?tory rate=84.21%). Intraoperative injury of lateral femoral cutaneous nerve developed in a case and obturator nerve damage oc?curred in another patient, respectively. Relevant symptoms were totally disappeared after two months’conservative treatment (in?cluding neurotrophic therapy combined with adduction exercise of the affected thigh). Iliac fossa hematoma occurred in a case and relevant symptom was eliminated after puncture. Conclusion Satisfactory clinical outcome of both?column acetabular fractures could be obtained by the anatomic quadrilateral surface plate through the Stoppa combined with iliac fossa approach.

11.
Chinese Journal of Trauma ; (12): 83-86, 2019.
Article in Chinese | WPRIM | ID: wpr-734176

ABSTRACT

Osteofascial compartment syndrome (OFCS) is clinically common and is well known to orthopedic surgeons.Clinicians attach great importance to OFCS because of its severe clinical consequences,and decompression of fascial compartment is often performed in emergency treatment.This article reviews the literature on the threshold of fascial compartment decompression proposed by many scholars in the past and discusses the problems in the clinical diagnosis of acute compartment syndrome,especially the inconsistent pressure thresholds as the indication for emergency decompression surgery.By observing calf fractures patients with tension blister,we found that the pressure of fascia decreased sharply upon the appearance of blisters.Meanwhile,the swelling gradually subsided as well as the clinical manifestations of pain and parasthsia.In view of the uncertainty of various thresholds of fascial decompression and self-decompression,the concepts of myofascial self-release law and muscle-swelling syndrome were first proposed.The author believes that when intracompartmental pressure rises to a point,some unknown mechanisms of fascia can achieve self-decompression.Therefore,no compartment syndrome will take place.We also emphasize that the ' muscle-swelling syndrome'should be strictly distinguished from the soft tissue necrosis caused by crush syndrome and acute limb vascular injury,so as to provide more precise treatment.We believe that without external restrictions such as casts,splints and compression bandages,the muscle-swelling syndrome can achieve self decompression by releasing the pressure in the compartment through tension blisters,and there is no need for fasciotomy.

12.
Article in Chinese | WPRIM | ID: wpr-813053

ABSTRACT

To investigate the clinical value of vaginal endoscopy in the diagnosis and treatment for vaginal and uterine diseases in young girls and adolescent girls. 
 Methods: The clinical data of 156 young girls and adolescent girls treated with vaginoscopy from February 2000 to August 2017 were analyzed retrospectively.
 Results: Of the 37 cases of young girls, the clinical symptoms were the most common in vaginal hemorrhage, accounting for 40.5% (15 cases), the second one in vaginal foreign body, accounting for 29.7% (11 cases), the third one in abnormal vaginal secretion, accounting for 24.3% (9 cases), and the last one in low abdominal pain, accounting for 5.4% (2 cases). The final diagnosis of vaginal foreign body was 13 cases (35.1%). Of the 119 adolescent girls, the clinical symptoms of the visit were the most common in menstrual abnormalities or irregular vaginal bleeding, accounting for 75.6% (90 cases). The final diagnosis of abnormal uterine bleeding-ovulatory dysfunction was the most common with 59 cases (49.6%), following by 16 cases of abnormal uterine bleeding-polyp, 2 cases of abnormal uterine bleeding-malignancy and hyperplasia, and 1 cases of abnormal uterine bleeding-coagulopathy. There were 37 cases (31.1%) of reproductive tract malformation. All 156 patients successfully completed vaginal endoscopy and operation with only 1 case of urinary system infection. No other complications occurred and no damage in the hymen. 
 Conclusion: Vaginal endoscopy is a safe, effective and near-noninvasive way for the diagnosis and treatment of vaginal or uterine diseases in young girls and adolescent girls. In the case of abnormal vaginal secretions and vaginal bleeding, it should be properly recommended to use the vaginal endoscopy for diagnosis and treatment.


Subject(s)
Adolescent , Endoscopy , Female , Foreign Bodies , Humans , Retrospective Studies , Uterine Hemorrhage , Vagina
13.
Chinese Journal of Orthopaedics ; (12): 781-788, 2019.
Article in Chinese | WPRIM | ID: wpr-802574

ABSTRACT

Objective@#To explore the clinical outcome an anatomic quadrilateral surface plate for both-column acetabular fractures through the Stoppa combined with iliac fossa approach.@*Methods@#A retrospective study of the patients with both-column acetabular fractures treated with an anatomic quadrilateral surface plate through the Stoppa combined with iliac fossa approach from November 2015 to June 2017 was performed. Nineteen patients including 16 males and 3 females met the inclusion criteria. The mean age of included patients was 49.11 years (range, 25-68 years). There were 8 cases of fall injury, 7 cases of car accident injury and 4 cases of crushing injury. There were 11 cases of C1 type injury, 7 cases of C2 type, and 1 case of C3 type according to Tile classification. Patients included in this study were both-column acetabular fractures according to Letournel-Judet classification. There were six cases associated with posterior wall detachment. Minimally Stoppa combined with iliac fossa approach was performed to manage the displaced fragments. First, the anteromedially displaced iliopubic fragment was managed with a reconstruction plate through iliac fossa approach. Then, the medially displaced ischiadic fragment could be fixed with an anatomic quadrilateral surface plate through the Stoppa approach. The reduction quality was assessed by postoperative radiographs and CT scans according to the criteria proposed by Matta. The function of hip joint was assessed by the Merle d'Aubigné-Posteal score modified by Matta.@*Results@#The average operation time and blood loss were 115.26 min (range, 90-160 min) and 534.21 ml (range, 300-1000 ml), respectively. The mean length surgical incision was 18.82 cm (range, 16-20 cm). Average follow-up time was 19.84 months (range, 12-28 months). According to Matta criteria of reduction quality, anatomic reduction was obtained in 14 cases, and satisfactory reduction was gained in 3 cases, while unsatisfactory reduction was found in 2 cases (satisfactory rate=89.47%). All fractures healed well and mean healing time was 3.32 months (range, 3-5 months). The mean Merle d’Aubigné-Posteal score modified by Matta was 16.95 (range, 13-18), including 12 cases of excellent, 4 cases of good, and 3 cases of fair (satisfactory rate=84.21%). Intraoperative injury of lateral femoral cutaneous nerve developed in a case and obturator nerve damage occurred in another patient, respectively. Relevant symptoms were totally disappeared after two months’ conservative treatment (including neurotrophic therapy combined with adduction exercise of the affected thigh). Iliac fossa hematoma occurred in a case and relevant symptom was eliminated after puncture.@*Conclusion@#Satisfactory clinical outcome of both-column acetabular fractures could be obtained by the anatomic quadrilateral surface plate through the Stoppa combined with iliac fossa approach.

14.
Article in Chinese | WPRIM | ID: wpr-851028

ABSTRACT

Objective: HPLC was used to determine the content of the polyphyllins VII, H, VI, II, III, I, and V in the rhizomes of multiple stems Paris polyphylla var. yunnanenesis from different places of origin, establishing HPLC fingerprint and comparative analysis HPLC fingerprint between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis. Methods: HPLC was performed on the column of Thermore C18 with mobile phase of acetonitrile-water gradient at the flow rate of 1 mL/min; The detection wave-length was 203 nm, column temperature was 30 ℃, and volume was 10 μL; Content of multiple stems P. polyphylla var. yunnanenesis. was measured by external standard and quantitative analysis of multi-components (QAMS), and one-way ANOVA was used to explore its content difference. The HPLC fingerprint of rhizome from multiple stems P. polyphylla var. yunnanenesis was established and compared with that of rhizome from P. polyphylla var. yunnanensis. Results: The determination results of 10 batches of rhizome of multiple stems P. polyphylla var. yunnanenesIs showed a good linear relationship in the linear range (r > 0.999 7), with an average recovery of 98.34%-99.34% and RSD ≦ 1.00%. There was a significant difference (P < 0.05) or highly significant difference (P < 0.01) about content of polyphyllins VII, H, VI, II, III, I, and V among different places of origin in the rhizomes of multiple stems P. polyphylla var. yunnanenesis, the total content of polyphyllins (I+ II + VI + VII)% ranging from 1.239%-6.236%, which was significantly higher than 0.60% of the quality control standard of Paridis Rhizoma prescribed by Chinese Pharmacopoeia. No significant difference was observed between the results of external standard and quantitative analysis of multi-components methods. The HPLC fingerprint similarity evaluation results showed that there were 12 common fingerprint peaks between multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis, and with the high similarity of HPLC fingerprints. Conclusion: The method was simple, accurate and reproducible, and can be used for the determination of polyphyllins VII, H, VI, II, III, I, and V in multiple stems of P. polyphylla var. yunnanenesis, total content of polyphyllins (I + II + VI + VII)% in multiple stems of P. polyphylla var. yunnanenesis was relatively higher and HPLC fingerprints of rhizome of multiple stems P. polyphylla var. yunnanenesis and P. polyphylla var. yunnanensis from different places of origin also have high similarity.

15.
China Pharmacy ; (12): 344-348, 2019.
Article in Chinese | WPRIM | ID: wpr-816886

ABSTRACT

OBJECTIVE: To study the effects of exogenous methyl jasmonate on the contents of 4 kinds of secoiridoid substance (gentiopicrin, swertiamain, sweroside and amarogentin) and biochemical indexes in the leaves of Gentiana rigescens, and to provide scientific evidence for the application of methyl jasmonate in standardized planting of G. rigescens. METHODS: 2, 4, 6, 8, 10 d after spraying 10, 50, 100, 200 mg/L methyl jasmonate (spraying amount was 500 mL) for G. rigescens strain, the leaves of medicinal material were collected as sample, and the other leaves without spraying methyl jasmonate were collected as control. HPLC method was used to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying 4 kinds of concentrations of methyl jasmonate for 10 d. The concentration of methyl jasmonate was optimized (the content of effective component was the highest). HPLC method was adopted to determine the contents of 4 kinds of secoiridoid substance in the leaves of G. rigescens after spraying the optimal concentration of methyl jasmonate for different time. The levels of relevant biochemical indexes (SOD, POD, CAT, MDA) were determined. RESULTS: 10 d after spraying 10, 50, 100, 200 mg/L methyl jasmonate for G. rigescens strains, the contents of 4 kinds of secoiridoid substance in the leaves were increased to different extent. Compared with untreated leaves, 100 mg/L methyl jasmonate had the best effect after spraying, and the contents of gentiopicrin, swertiamain and sweroside in treated leaves were 1.88, 2.36 and 1.87 times of those in untreated leaves, respectively (P<0.05). 2, 4, 6, 8, 10 d after spraying 100 mg/L methyl jasmonate, the contents of 4 kinds of secoiridoid substance in treated leaves were higher than untreated leaves at corresponding stage; the content of secoiridoid had significant difference after spraying for 4 d (P<0.05). The contents of active components in general were relatively high after spraying for 6 d, and the contents of gentiopicrin, swertiamain, sweroside and amarogentin in treated leaves were 1.88, 1.88, 1.47, 1.82 times of those in untreated leaves, respectively (P<0.05). The levels of relevant biochemical indexes in treated leaves were increased significantly since 4 d of spraying, compared with untreated leaves (P<0.05). CONCLUSIONS: After spraying 100 mg/L methyl jasmonate for 6 d, the contents increase of 4 kinds of secoiridoid substance in the leaves of G. rigescens are most obvious, which may be associated with improving the levels of related biochemical indexes.

16.
Article in Chinese | WPRIM | ID: wpr-807274

ABSTRACT

Focal segmental glomerulosclerosis is a type of glomerulopathy, which is named after the pathological characteristics of kidney. It is an important part of glomerular disease, and also a common chronic kidney disease in clinical practice.A number of epidemiological studies have shown that the incidence of FSGS has increased year by year and its prognosis is not satisfying. At present, there is still a lack of comprehensive understanding of the occurrence and development of FSGS. This article summarizes the important findings from the research and exploration of FSGS pathogenic factors in recent years in order to provide reference value for the diagnosis and treatment of FSGS.

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Article in Chinese | WPRIM | ID: wpr-806454

ABSTRACT

Objective@#To analyze the base situation and influential factors of late diagnosis among newly identified HIV/AIDS cases in Anhui Province from 2011 to 2015.@*Methods@#Database information of the newly identified HIV/AIDS cases in Anhui Province from 2011 to 2015 were downloaded from the National HIV/AIDS Comprehensive Information System of China's disease prevention and control information system. To analyze the data including basic information, sample source, route of HIV transmission, population mobility, venereal disease, death and first CD4 count; and the number of 7 073 cases were classified according to late diagnosis and non-late diagnosis criteria. The Chi-square test and logistic regression analysis were used to analyze the influential factors of HIV late diagnosis.@*Results@#A total of 7 073 newly identified HIV/AIDS cases were analyzed, and the mean age was (38.5±15.0) years. The proportion of late diagnosis in all counted cases was 41.7% (2 949/7 073); from 2011 to 2015, the proportions of late diagnosis were 59.7% (485/812), 46.5% (531/1 141), 42.7% (587/1 376), 36.1% (609/1 686), and 35.8% (737/2 058), respectively. Compared with the 0 to 19 years group, the 40 to 59 years group and over 60 years old group have higher risk of late diagnosis (OR=2.68, 95%CI: 1.94-3.71; OR=2.18, 95%CI: 1.53-3.10, respectively). Compared with the high education group, the illiterate and primary school education group have higher risk of late diagnosis (OR=1.74, 95%CI: 1.36-2.22; OR=1.64, 95%CI: 1.34-2.01, respectively). Compared with other sample sources, medical institutions have higher risk of late diagnosis (OR=2.64, 95%CI: 2.28-3.05). Compared with migrant population, the resident population have higher risk of late diagnosis (OR=1.80, 95%CI: 1.53-2.11).@*Conclusion@#The proportion of late diagnosis among newly identified HIV/AIDS cases in Anhui province was relatively high from 2011 to 2015. The main risk factors of late diagnosis included cases reported by medical institutions, resident population, over 40 years old age group and low education level.

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Journal of Medical Postgraduates ; (12): 622-627, 2018.
Article in Chinese | WPRIM | ID: wpr-700884

ABSTRACT

Objective The loop electrosurgical excision procedure (LEEP) is a major option for the treatment of cervical in-traepithelial neoplastic (CIN) lesions,but reports are rarely seen on the consistency of the result of post-LEEP pathology with that of preoperative biopsy as well as on the postoperative pathological upgrading of related factors. This study analyzes the consistency of the result of preoperative biopsy with that of post-LEEP pathology for CIN lesions and the risk factors for residual and/or recurrence after LEEP. Methods A retrospective study was conducted on 1 089 patients with CIN lesions treated by LEEP in our hospital from May 2014 to A-pril 2017. All the patients underwent liquid-based thin-layer cytology (TCT) and vaginoscopic biopsy preoperatively,followed by analysis of the results of TCT,the consistency of the result of preoperative bi-opsy with that of post-LEEP pathology,and the risk factors for residu- al and/or recurrence after LEEP. Results Preoperative biopsy showed 447 cases of low-grade squamous intraepithelial lesion (LSIL),among which TCT revealed 85 cases negative for intraepithelial lesion and malignancy (NILM),with a false negative rate of LSIL of 19.0%. Of the 645 cases of high-grade squamous intraepithelial lesion (HSIL) revealed by preoperative biopsy,TCT manifes-ted 95 cases of NILM,with a false negative rate of HSIL of 14.7%,which was significantly lower than that of LSIL (P<0.05). Preop-erative biopsy showed 447 cases of LSIL,among which post-LEEP pathology manifested 316 cases of LSIL (70.69%) and 51 cases of HSIL (11.4%). Of the 635 cases of HSIL revealed by preoperative biopsy,post-LEEP pathology exhibited 4 cases (0.63%) of inva-sive cervical cancer (ICC). Multivariate analysis showed the risk factors for pathological upgrading after LEEP were sexual partners >2 (OR=0.139,95% CI: 0.034-0.561) and LSIL in TCT (OR=0.111,95% CI: 0.040-0.310) (P<0.05) and those for postopera-tive recurrence included positive incision margin (OR=2.970,95% CI: 1.010-8.733) and persistent human papilloma virus (HPV) infection (OR=5.446,95% CI: 3.109-9.540). Preoperative HPV16 infection was correlated with lesion residual and/or recurrence after LEEP (P<0.05). Conclusion LEEP can significantly improve the diagnosis rate of HSIL in low-grade CIN patients with high risk factors. The risk factors for lesion residual and/or recurrence after LEEP include HPV16 infection,lesion involvement of the inci-sion margin,and persistent HPV infection.

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Article in Chinese | WPRIM | ID: wpr-775407

ABSTRACT

Through the determination of the growth, physiological and active components of Glechoma longituba, the effect of soil bulk density on growth and quality of G. longituba was explored. Through pot experiment, six different bulk density treatments were set for 0.8, 0.9, 1.0, 1.1, 1.2, 1.3 g·cm⁻³ , the growth index, physiological index and the content of active components were determined. The results showed that the growth indexes and biomass of G. longituba firstly increased and then decreased with the increase of soil bulk density. The dry weight of G. longituba achieved the maximum 5.70 g in the treatment of 1.0 g·cm⁻³ . The photosynthetic pigments, soluble sugars and free amino acids firstly increased and then decreased with the increase of soil bulk density. The suitable soil bulk density was beneficial to the accumulation of photosynthetic pigment, soluble protein and free amino acid synthesis. The content of malondialdehyde increased gradually with the increase of soil bulk density. The contents of ethanol-soluble extractives of G. longituba were greater than 25%, meeting its quality criterionin Chinese Pharmacopoeia 2015, achieved the maximum of 40.66% in the treatment of 1.0 g·cm⁻³ . The total flavonoids content of G. longituba increased gradually with the increase of soil bulk density, triterpenic acid and phenolic acids decreased gradually with the increase of soil bulk density. Soil bulk density has a significant effect on the growth and quality of G. longituba, and it is conducive to the growth and its medicinal quality at the soil bulk density of 1.0 g·cm⁻³.


Subject(s)
Flavonoids , Lamiaceae , Chemistry , Photosynthesis , Pigments, Biological , Plant Extracts , Soil
20.
Article in Chinese | WPRIM | ID: wpr-709707

ABSTRACT

Objective To determine the risk factors for severe complications after total aortie areh replacement.Methods Patients with acute type A aortic dissection of both sexes,scheduled for elective total aortic areh replacement,were selected.According to whether or not postoperative severe complications occurred during hospitalization,the patients were divided into either postoperative severe complications group or non-postoperative severe complication group.Factors including age,gender,body mass index,preoperative complications (Marfan syndrome,hypertension and diabetes mellitus),preoperative left ventricular ejection fraetion,levels of serum N-terminal pro-brain natriuretic peptide,hemoglobin and serum alanine aminotransferase,aspartate aminotransferase and creatinine,international normalized ratio,operation time,cardiopulmonary bypass time,selective antegrade cerebral perfusion time,regional cerebral oxygen saturation (rSO2),etc.were recorded.The risk factors of which P values were less than 0.05 would enter the multivariate logistie regression analysis to stratify postoperative severe complications-related risk factors for this type of patients.Results A total of 98 patients were enrolled in this study.Of the 98 patients,28 patients developed posloperative severe complications,and the incidence was 29c%.The results of logistic regression analysis showed that age>60 yr,△rSO2-min<80% throughout the surgery and △rSO2-min<80% during hemostasis were independent risk factors for severe complications after total aortie arch replacement.Conclusion Age >60 yr,△rSO2-min<80% throughout the surgery and △rSO2-min<80% during hemostasis are independent risk factors for severe complications after total aortic arch replacement.

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