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1.
Article in Chinese | WPRIM | ID: wpr-847089

ABSTRACT

OBJECTIVE: Vacuum-sealing drainage has the effect of promoting wound healing and preventing infection. At present, a large number of studies have confirmed the significant efficacy of vacuum-sealing drainage combined with antibiotic bone cement in the treatment of chronic osteomyelitis, but the corresponding evidence is still lacking. A meta-analysis was performed on the effect of treatment of chronic osteomyelitis by vacuum-sealing drainage combined with antibiotic bone cement. METHODS: CNKI, VIP, Wanfang, PubMed, Cochrane Library and EMbase were searched to find randomized controlled trials of vacuum-sealing drainage combined with antibiotic bone cement for the treatment of chronic osteomyelitis. The retrieval time was from inception to February 2020. The quality of the included literature was strictly evaluated, and the RevMan 5.3 software was used for meta-analysis of the included research. RESULTS: Ten randomized controlled trials involving 637 patients with chronic osteomyelitis were included, with the treatment group of 313 cases and control group of 324 cases. Due to the special wound therapy, it was difficult to implement blind method clinically, so there was partial implementation bias. (2) Metaanalysis results showed that the recurrence rate of chronic osteomyelitis treated with vacuum-sealing drainage with antibiotic bone cement was lower than that in the control group (OR=0.20, 95%CI:0.11-0.37, P < 0.000 01). In the healing rate (OR=3.44, 95%CI:2.01-5.92, P < 0.000 01), healing time (MD=-11.05, 95%CI:-18.59 to-3.52, P=0.004), duration of antibiotic use (MD=-12.36, 95%CI:-13.27 to-11.44, P < 0.000 01), visual analogue scale score (MD=-1.94, 95%CI:-2.80 to-1.08, P < 0.000 01), times of dressing change (MD=-5.78, 95%CI:-6 .64 to-5.12, P < 0.000 01), bone absorption time (MD=-0.81, 95%CI:-0.98 to-0.64, P < 0.000 01), and bone healing time (MD=-1.62, 95%CI:-2.22 to-1.03, P < 0.000 01) were all better in the vacuum-sealing drainage with antibiotic bone cement group than those in the control group. CONCLUSION: The combination of vacuum-sealing drainage and antibiotic bone cement in the treatment of chronic osteomyelitis has a significant advantage over the traditional treatment, which is worthy of clinical promotion. Limited by the quantity and quality of the included studies, the above conclusions need to be verified by more high-quality clinical studies.

2.
Chinese Medical Journal ; (24): 806-813, 2021.
Article in English | WPRIM | ID: wpr-878036

ABSTRACT

BACKGROUND@#Mastectomy techniques have been extended to nipple-sparing mastectomy (NSM). This study aimed to assess the actual application of NSM in China and identify the factors influencing postoperative complications.@*METHODS@#The clinical data of 615 patients (641 surgeries) undergoing NSM from January 1st, 2018 to December 31st, 2018 at 28 centers nationwide were retrospectively analyzed to obtain the rate of NSM and investigate factors related to NSM surgery.@*RESULTS@#The proportion of NSM surgery performed in this study was 2.67% (17/641). Malignant breast tumors accounted for the majority of NSM surgery (559/641, 87.2%). A total of 475 (77.3%) patients underwent NSM combined with reconstructive surgery. The rate of reconstruction decreased with age in our study, and implants were the most common option (344/641, 53.7%) in reconstruction. Radial incision was the most selected method regardless of reconstruction. However, for those who underwent reconstruction surgery, 18.4% (85/462) of cases also chose curvilinear incision, while in the simple NSM surgery group, more patients chose circumareolar incision (26/136, 19.1%). The tumor-to-nipple distance (TND) influenced postoperative complications (P = 0.004). There were no relationships between postoperative complications and tumor size, tumor location, histologic grade, molecular subtype, nipple discharge, and axillary lymph nodes.@*CONCLUSIONS@#NSM surgery is feasible and only TND influenced postoperative complications of NSM surgery. But the proportion of NSM surgery performed is still low in nationwide centers of China. The selection criteria for appropriate surgical methods are important for NSM in clinical practice. To optimize clinical applications of NSM, further multicenter prospective randomized controlled studies are needed.@*TRIAL REGISTRATION@#ChiCTR.org.cn, ChiCTR1900027423; http://www.chictr.org.cn/showprojen.aspx?proj=38739.


Subject(s)
Breast Neoplasms/surgery , China , Humans , Mammaplasty , Mastectomy , Nipples/surgery , Prospective Studies , Retrospective Studies
3.
Article in Chinese | WPRIM | ID: wpr-856266

ABSTRACT

Objective: To evaluate the effectiveness of the nose ring drain (NRD) in treatment of severe diabetic foot infection. Methods: The clinical data of 35 patients with severe diabetic foot infection who were treated with NRD between June 2017 and June 2019 were analyzed retrospectively. There were 24 males and 11 females with an average age of 54.5 years (range, 28-82 years). All of them were type 2 diabetic patients. The diabetes duration was 3-20 years, with an average of 9.4 years. The diabetic foot duration was 4 months to 2 years, with an average of 1.16 years. There were 16 cases of left foot and 19 cases of right foot. According to Wagner's grading, there were 11 cases of grade 2, 20 cases of grade 3, and 4 cases of grade 4, all of which were moderate and severe infection of diabetic foot wound. Postoperative wounds were treated with "nibble-like" debridement until the patient's epidermis regenerated and healed. During the treatment process, the indexes of bacterial culture type of wound secretions, duration of antibiotic therapy, wound healing method, healing time, amputation rate, and other indicators were analyzed and summarized. Results: All 35 patients were followed up 3-6 months, with an average of 4.5 months. Postoperative bacterial culture of wounds showed that 5 cases of Staphylococcus aureus, 4 cases of Pseudomonas aeruginosa, 5 cases of Escherichia coli, 3 cases of Enterobacter cloacae, 3 cases of coagulase-negative Staphylococcus, and 15 cases of other types were detected. The duration of antibiotic therapy ranged from 3 to 15 days, with an average of 9.1 days. The wound was autolytically healed without skin grafting, and the healing time was 62-82 days, with an average of 72.3 days. During the follow-up, 3 cases (8.6%) had amputation due to the patient's poor blood glucose control, which led to a large spread of infection. In addition, among the other patients with wound healing, there was no recurrence of wound infection or new ulcer on the original surface. Conclusion: The NRD is a simple operation for treatment of severe diabetic foot infection, which can effectively control wound infections and promote wound healing and regeneration without skin grafting.

4.
Article in English | WPRIM | ID: wpr-772243

ABSTRACT

OBJECTIVE@#To evaluate the possible association between radon exposure and kidney cancer.@*METHODS@#We performed a systematic review and a meta-analysis based on random effect models to provide a pooled association measure.@*RESULTS@#We subjected 8 studies (overall relative risks and 95% confidence intervals: 1.01, 0.72 to 1.43, I2 = 64.4%) to meta-analysis. Subgroup analysis revealed a marginally significant association between radon exposure and kidney cancer in studies conducted in Europe. Two population-based studies provided no evidence for the increased risk of kidney cancer in the general population.@*CONCLUSION@#The association between radon and kidney cancer remains unclear but cannot be excluded because of its biological plausibility and the limited number and quality of existing studies. Additional data from the general population and well-designed miner cohort studies are needed to reveal the real relationship between radon exposure and kidney cancer.


Subject(s)
Cohort Studies , Environmental Exposure , Humans , Kidney Neoplasms , Radon , Toxicity
5.
Article in Chinese | WPRIM | ID: wpr-700195

ABSTRACT

Objective To investigate the expression and clinicopathological significance of Twist1 gene in esophageal squamous cell carcinoma(ESCC),and its effect on migration and invasion of ESCC.Methods The ESCC tissues and adjacent normal tissues of 30 ESCC patients having underwent surgery were selected. The expression levels of Twist1 mRNA and protein were detected by real time fluorescence quantitative polymerase chain reaction (RT- qPCR) and Western blot methods. The expression level of Twist1 mRNA in adjacent normal tissues was used as control,the relative expression levels of Twist1 mRNA in 3 ESCC cell lines K70,K140 and EC109 were detected,and the relationship between the expression level and clinicopathological features was analyzed. ESCC cell line EC109 was selected for cell scratch test and Transwell chamber invasion assay respectively.Twist1 small interfering RNA and control double stranded nonsense RNA were transfected respectively, and the changes were compared.Results The expression level of Twist1 mRNA in ESCC tissue was significantly higher than that in adjacent normal tissue(1.91 ± 0.93 vs.0.54 ± 0.26),and there was statistical difference(P<0.01). The relative expression levels of Twist1 mRNA in 3 ESCC cell lines K70, K140 and EC109 were significantly higher than those in adjacent normal tissue (2.30 ± 0.34, 1.78 ± 0.28 and 4.37 ± 0.25 vs. 1.00 ± 0.48), and there were statistical differences (P<0.05). The expression level of Twist1 protein in ESCC tissue was significantly higher than that in adjacent normal tissue (1.35 ± 0.66 vs. 0.25 ± 0.05), and there was statistical difference (P<0.05). The relative expression level of Twist1 mRNA in ESCC tissue had correlation with lymph node metastasis and invasion degree(P<0.05).The expression levels of Twist1 mRNA in ESCC cell lines EC109 after transfected Twist1 small interfering RNA and double stranded nonsense RNA were 0.45 ± 0.16 and 1.00 ± 0.20, the express levels of Twist1 protein were 0.24 ± 0.09 and 0.58 ± 0.10,and there were statistical differences(P<0.05).The cell scratch test result showed that the migration rates of 36 and 72 h of ESCC cell line EC109 were significantly lower than those of the control double stranded nonsense RNA,after Twist1 expression was down regulated by RNA interference:(32.6 ± 4.7)% vs.(16.2 ± 6.0)% and(71.9 ± 4.7)% vs.(53.2 ± 6.6)%,there were statistical differences(P<0.05).The Transwell chamber invasion assay result showed that the total number of cells in the bottom of Twist1 through the small interfering RNA of EC109 was lower than that of the transfected control double stranded nonsense RNA (92.5 ± 8.1 vs. 32.7 ± 7.5), and there was statistical difference (P<0.05). Conclusions Twist1 gene may be highly abnormally expressed in ESCC, which has some correlation with invasion and lymph node metastasis,and may have some influences on the migration and invasion of ESCC.

6.
Article in Chinese | WPRIM | ID: wpr-667655

ABSTRACT

Objective To investigate the efficacy and safety of Bushen Huoxue Decoction in the treatment of carotid atherosclerotic plaque. Methods Totally 50 patients of carotid atherosclerotic plaque were collected to conduct health education. At the same time, they were given Bushen Huoxue Decoction, one dosage a day, twice a day, decoction, for 12 weeks. The correlation indexes of carotid artery before and after treatment were observed, and TCM syndrome integrals were evaluated before and after 4, 8 and 12 weeks treatment. FBG, TC, LDL-C, CRP HCY, IL-6, IGF-1, and OPG were detected before and after treatment. Hematuria routine, liver and renal function were monitored. Results After treatment, the Crouse score and the plaque grade score of carotid atherosclerotic plaque showed a tendency to decrease, without statistical significance (P=0.060, P=0.336). Carotid maximal plaque area and modified total score of carotid atherosclerotic plaque were significantly reduced compared with that before treatment (P<0.05). TCM syndrome integrals were significantly reduced compared with that before treatment (P<0.05), and the scores decreased significantly with the prolongation of treatment time. TCM syndrome integrals after 4, 8, and 12 weeks were significantly reduced compared with that before treatment (F=160.352, P<0.001). Total effective rate of treatment in the efficacy of TCM syndrome was 85.11% (40/47). Serum levels of FBG and TC decreased significantly after the treatment compared with that before the treatment, with statistical significance (P<0.05). Serum levels of LDL-C, HCY, CRP, IL-6, IGF-1, and OPG all showed a tendency to decrease, without statistical significance (P>0.05). No abnormality in hematuria routine, liver and renal function examinations were found during the research. Conclusion Bushen Huoxue Decoction has obvious clinical efficacy for carotid atherosclerotic plaque, which can improve clinical symptoms of patients, it also could stabilize and reduce plaque with high safety.

7.
Article in Chinese | WPRIM | ID: wpr-614549

ABSTRACT

Objective To explore the gray matter changes in aggressive patients with schizophrenia,and the relationship between the gray matter and aggression in patients.Methods Eighteen aggressive patients with schizophrenia (SZ1),18 age-and gender-matched un-aggressive patients with schizophrenia (SZ2) and 18 normal controls (NC) were enrolled in the study.Then a 3.0 T magnetic resonance imaging (MRI) scan was conducted for each participant.The voxel-based morphometry (VBM) approach and the Chinese version of Buss & Perry aggression questionnaire (B&P) were used to explore imaging data and to assess the aggression,respectively.Results Compared with NC,patients with schizophrenia showed changes in gray matter volume (GMV) in the frontal,temporal and the occipital lobes (P<0.05,AlphaSim corrected).Compared with SZ2,SZ1 showed increased GMV in the right supramarginal gyrus,right postcentral gyms,bilateral insula and orbito-frontal gyri (P<0.05,AlphaSim corrected).The GMV of the right insula,right postcentral gyms and right supramarginal grus were positively associated with B&P scores in patients with schizophrenia (P<0.01,AlphaSim corrected),respectively.Conclusions These preliminary findings support that the aggression in schizophrenia is associated with GMV changes of brain regions in patients with schizophrenia.The right postcentral gyrus,the right insula and the right supramarginal gyrus may be involved in the neural mechanism of aggression in schizophrenia.

8.
Article in Chinese | WPRIM | ID: wpr-614190

ABSTRACT

Objective To observe the efficacy and safety of Bushen Huoxue Prescription in the treatment of kidney deficiency and blood stasis type primary osteoporosis. Methods Totally 50 patients with kidney deficiency and blood stasis type primary osteoporosis were collected. They were given Bushen Huoxue Prescription granules twice a day, taking with water, for 12 weeks. Before and after treatment, the femoral neck, Ward's area, L1–4 bone mineral density (BMD) were measured. VAS and TCM syndrome integrals were evaluated before and 4, 8 and 12 week of treatment. The level of serum osteocalcin (OC), β-C-terminal telopeptides of type I collagen (β-CTX) and osteoprotegerin (OPG) were detected before and after treatment. Hematuria routine, liver and kidney function were under safety testing before and after treatment. Results Bushen Huoxue Prescription could significantly increase BMD of left femoral neck and Ward's triangle (P=0.001, P=0.044). L1–4 BMD increased, without statistical significance (P=0.172). The total effective rate was 60.870% (28/46) for L1–4 and left femoral neck and 58.696% (27/46) for Ward's triangle. The VAS and TCM syndrome integral decreased significantly 4 weeks treatment compared with that before the treatment (P<0.01), and the scores decreased significantly with the prolongation of treatment time (F=159.690, P<0.001; F=163.970, P<0.001). After 12 weeks, treatment, the total effective rate for TCM syndromes was 78.26% (36/46). Serum levels of OC and β-CTX showed a tendency to increase, while serum levels of OPG showed a tendency to decrease, without statistical significance (P=0.087, P=0.091, P=0.419). There was no serious adverse reaction in hematuria routine and liver and kidney function. Conclusion Bushen Huoxue Prescription has obvious clinical efficacy for kidney deficiency and blood stasis type primary osteoporosis, which can improve the patients' BMD, improve bone metabolism, relive bone pain, with high level of safety.

9.
Article in Chinese | WPRIM | ID: wpr-610650

ABSTRACT

Objective To establish the enzyme-linked immunosorbent assay (ELISA) methods for the quantitative determination of IgG antibodies against diphtheria (DT) and tetanus (TT).MethodsPurified diphtheria toxiod and tetanus toxoid were respectively used as the coating antigens,the human-derived serum antibody standard substance of DT and TT served as the standard substance.The dose-response curves of the tested samples and standard substance were fitted.Then the two quantitative ELISA methods for determining the antibody to DT (Anti-DT) and antibody to TT (Anti-TT) were established with the parallel lines method.Then the methodological verification and application study were conducted.Results The validation results of the two quantitative ELISA measurement methods were in accordance with the regulations.The quantity limit of ELISA method for quantitative detection of Anti-DT demonstrated to be 0.084 mIU/mL,its average recovery rate was 97.6%.The intra-assay coefficient of variation(CV) and inter-assay CV of this Anti-DT assay were ≤ 3.40% and ≤5.05%,respectively.The quantity limit of ELISA method for quantitative detection of Anti-TT demonstrated to be 0.175 mIU/mL,its average recovery rate was 97.5%.The intra-assay CV and inter-assay CV of this Anti-TT assay were ≤ 2.42% and ≤5.58%,respectively.These two methods were applied for the immunogenicity evaluation after infantile basic immunization by diphtheria and tetanus vaccines.Conclusion The two established quantitative ELISA methods demonstrate high accuracy and good reproducibility,which are suitable for the ordinary laboratory to carry out the work and can be used in the serological effect evaluation after diphtheria and tetanus vaccine immunization and epidemiological study of diphtheria and tetanus disease.

10.
Article in Chinese | WPRIM | ID: wpr-513058

ABSTRACT

Objective To explore the mechanism of SH?SY5Y mitochondrial dysfunction treated by manganese to find a new potential therapeutic target. Methods Transmission Electron Microscopy(TEM)to observe the morphology of mitochondria. Cell treated with 250μmol/L for periods of time(2 h, 4 h, 6 h)while mitochondrial membrane potential(MMP)and ROS can be detected by FCM and fluorescence microplate reader. Results After treating with MnCl2 in 6 h, TEM images showed early vacuoles, lamellar structures of SH?SY5Y cells. Then test the mitochondrial membrane potential and showed that MMP would be decreased gradually. Meanwhile, analysis showed that in comparison with control, treatment group had a higher ROS level respectively (P < 0.05). Conclusion MnCl2 can cause mitochondrial damage through a mechanism closely related to disrupt the MMP or generate abundant ROS.

11.
Article in Chinese | WPRIM | ID: wpr-852720

ABSTRACT

Objective: To study the antidepressant effect of Anacycli Pyrethri Radix extract (APRE) in chronic unpredictable mild stress (CUMS) model and drug-induced depression model. Methods: The chronic unpredictable stimulus was used to establish exogenous chronic stress depression model in rats. The sucrose preference, open-field test, forced swimming test and tail suspension test were used to observe the behavioral changes of rats. Serum levels of corticosterone and 5-HT and DA of hippocampus were analyzed using ELISA. Reserpine reversal test, 5-HTP induced head-twitches test and yohimbine induced lethality were used to establish endogenous mouse depression model; Blepharoptosis, akinesia, head-twitch cunts and mortality were observed to study he antidepressant effect of APRE. Results: In the chronic unpredictable stress model, APRE could increase the sucrose intake, crossing numbers and rearing numbers obviously, the immobility time was reduced, the level of the serum corticosterone was reduced significantly, the 5-HT and DA of hippocampus were increased. In the drug-induced depression model, blepharoptosis and akinesia incuced by reserpine were inhibited, head-twitches induced by 5-HTP was significantly increased, but there was no effect on mortality induced by yohimbine. Conclusion: APRE has obvious antidepressant effect possibly by decreasing corticosterone level and promoting 5-HT nerve function.

12.
Article in Chinese | WPRIM | ID: wpr-500726

ABSTRACT

Objective To study postoperative delirium in elderly patients.Methods We investigate the morbidity of postoperative delirium in 142 elderly patients (≥ 60 years)after gastrointestinal surgery by using Confusion Assessment Method (CAM) and Delirium Rating Scale Revised-98 (DRS-R98) scores.Data were analyzed using Student's t test and Chi-squaretest respectively with SPSS 19.0.Results Of 142 patients,delirium was diagnosed in 36 patients(25.4%),delirium developed in 4,7,17,7,1 patients in posto perative 1,2,3,4-7,7 + days respectively.There were significant difference in hospital stay:17.7 ± 2.6 days (postoperative delirium) and 13.4 ± 2.3 days (no postoperative delirium),t =4.608,P =0.000 1.The postoperative complications (52.8% / 23.6%,x2 =10.710,P =0.001) and ICU admission (22.2%/6.6%,x2 =6.939,P =0.008) significantly increased.Conclusions Postoperative delirium is recognized as one of the most common surgical complications in elderly patients with gastrointestinal surgery leading to other major postoperative complications,and prolonged hospitalization.

13.
Article in Chinese | WPRIM | ID: wpr-269968

ABSTRACT

<p><b>OBJECTIVE</b>To explore the concentration-response relationship between ambient concentration of PM2.5 and daily total hospital emergency room visits in Beijing during 2012 and 2013. This study also examined the effects of ambient PM2.5 during heavy polluted days on emergency room visits compared with the light polluted days.</p><p><b>METHODS</b>We collected the daily meteorological factors monitoring data and concentrations of air pollutants in Beijing during October 1, 2012 to December 31, 2013. We also collected the daily emergency room visits from a tertiary hospital in Beijing in the same time period. Generalized additive model was fitted to estimate the association between the ambient PM2.5 and the hospital emergency room visits, by using the smooth function to adjust long term trend of time, public holidays and day of week. In addition, constrained piecewise linear function was then used to estimate the excess risk for different segment of concentration-response function.</p><p><b>RESULTS</b>The annual average concentration of PM2.5 was 90.9 µg/m(3) during October 1, 2012 and December 31, 2013. There were total 64 260 cases for total emergency room visits, of which respiratory disease had 9 849 cases and cardiovascular disease had 11 168 cases. PM2.5 was positive related with PM10, NO2 and SO2. The corresponding correlation coefficients were 0.87, 0.78 and 0.62, respectively (P<0.05). And PM2.5 was positively related with relative humidity, with correlation coefficient 0.45 (P<0.05). But PM2.5 was negatively related with mean temperature (r=-0.17, P< 0.05) and wind speed (- 0.32, P<0.05). In the single polluted model, after adjusting the effects of temperature, relative humidity and wind, every 10 µg/m(3) increase of concentration of ambient PM2.5, the corresponding excess risk of daily emergency room visits was 0.25% (95% CI: 0.07-0.43). In the two-pollutant model PM2.5+SO2 and PM2.5+NO2, every 10 µg/m(3) increase of concentration of ambient PM2.5, the corresponding excess risk of daily emergency room visits were 1.07% (95%CI:0.83-1.30) and 0.56% (95%CI: 0.32-0.80) respectively, which were higher than the effect in single pollutant model. Average concentration of ambient particulate matters (PM2.5) was 204.16 µg/m(3) during heavy pollution, higher than control period (85.24 µg/m(3)). When PM2.5 as the primary air pollutants during heavy polluted days, we observed a significant increase in emergency room visits, and the odd ratios was 1.16 (95% CI:1.09-1.22).</p><p><b>CONCLUSION</b>There were positive correlation between high concentration of ambient particulate matters (PM2.5) and increasing daily emergency room visits. Especially during the heavy polluted days, the effects of elevated concentration of PM2.5 on hospital emergency room visits were much larger.</p>


Subject(s)
Air Pollutants , Beijing , Cardiovascular Diseases , Diagnosis , Emergency Service, Hospital , Humans , Meteorological Concepts , Particulate Matter , Respiratory Tract Diseases , Diagnosis , Temperature , Tertiary Care Centers
14.
Article in English | WPRIM | ID: wpr-277944

ABSTRACT

Objective To explore the diffusion pattern of tumor markers (TM) from serum to cerebrospinal fluid (CSF) via the blood-brain barrier in patients with elevated serum tumor markers (TM).Methods Inpatients receiving lumbar puncture during hospitalization in our center from January 1, 2013 to December 31, 2015 were divided into study group (n=181) and control group (n=251). The study group consisted of patients with elevated serum TMs but without malignant central nervous system diseases. The control group consisted of patients with normal serum TM levels and without malignant diseases. TMs measured in the study group included elevated serum alpha-fetoprotein (AFP) (n=0), carcinoembryonic antigen (CEA) (n=26), carcinomic antigen(CA)125 (n=39), CA15- 3 (n=3),CA19- 9 (n=19), CA724 (n=47), CYFRA21- 1 (n=49), and SCC (n=17).Levels of TMs in the CSF of study group was compared with that of control group.Results Median CEA (U=0.00,P=0.00),CA19- 9 (U=0.00,P=0.00),CA15- 3 (U=0.00,P=0.04),SCC (U=0.00,P=0.00),CA125 (U=0.00,P=0.00),CA72- 4 (U=3.00,P=0.00)),and CYFRA21- 1 (U=0.00,P=0.00) in CSF were significantly lower than the corresponding serum TM levels in the study group.There was no significant difference between study group and control group for the CSF level of CEA (U=3091.00,P=0.18),CA19- 9 (U=1897.00,P=0.14), CA15- 3 (U=373.50,P=0.91)and SCC (U=1925.50,P=0.76). CSF CA125 (U=2188.00,P=0.00) and CA724 (U=1279.00,P=0.00) levels in the study group were lower than those in control group. CSF level of CYFRA21- 1 (U=1826.50,P=0.00) in study group was higher than that in control group;however, it was still lower than the upper limit of reference value. Conclusion In patients with elevated serum CEA, CA19- 9, CA15- 3, SCC, CA125, and CA72- 4 levels, transblood-brain-barrier diffusion of TMs from serum to CSF is highly unlikely.


Subject(s)
Biomarkers, Tumor , Cerebrospinal Fluid , CA-125 Antigen , Cerebrospinal Fluid , CA-19-9 Antigen , Cerebrospinal Fluid , Carcinoembryonic Antigen , Cerebrospinal Fluid , Case-Control Studies , Central Nervous System Neoplasms , Cerebrospinal Fluid , Diagnosis , Humans , Mucin-1 , Cerebrospinal Fluid , Reference Values
15.
Article in Chinese | WPRIM | ID: wpr-604416

ABSTRACT

Objective To study the clinical effect of different tracheal intubation in patients with severe brain injury.Methods 82 patients with severe brain injury were selected and randomly divided into observation group and control group,each group had 41 cases.The observation group was given the dexmedetomidine induction combined with surface anesthesia on the basis of endotracheal intubation,and the control group was given traditional endotracheal intubation.The effects of the two groups were compared.Results The intubation time of the observation group was (26.7 ±13.8)s,which was significantly lower than (41.6 ±17.9)s of the control group(t =4.221,P =0.000).There were no differences in cardiovascular response indices between the two groups before intubation(t =0.122,P =0.903;t =0.296,P =0.768;t =1.128,P =0.263).After 10min,HR and MAP levels were significantly lower in the observation group than those in the control group(t =3.326,P =0.0.001;t =2.354,P =0.021).In the observation group,HR,MAP after intubation were lower than before intubation(t =2.548,P =0.013;t =3.626,P =0.000),the SpO2 of the two groups was higher than that before intubation(t =30.622,P =0.000;t =38.797,P =0.000),there were no differences in HR and MAP before and after intubation in the control group(t =0.846,P =0.400;t =1.824,P =0.072).There were no differences between the two groups before intubation(t =0.183,P =0.856;t =0.000,P =1.000;t =1.132,P =0.261),CK -MB,cTnT,BNP were significantly lower than those in the control group after 12h,respectively(t =2.030,P =0.046;t =2.264,P =0.026;t =3.785,P =0.000).CK -MB, cTnT,BNP were significantly lower than the control group after intubation in the two groups(t =7.845,P =0.000;t =8.591,P =0.000;t =22.757,P =0.000;t =5.525,P =0.000;t =2.264,P =0.000;t =22.149,P =0.000).The BCS and SS scores in the observation group were significantly higher than those in the control group(t =5.739,P =0.000;t =5.264,P =0.000).Conclusion The dexmedetomidine induction combined with topical anesthesia based uplink tracheal intubation scheme has significant effect,safety is good,it is worth of clinical application.

16.
Article in Chinese | WPRIM | ID: wpr-355318

ABSTRACT

<p><b>OBJECTIVE</b>To investigate effect of CD4(+) CD25(+) Foxp3(+) Tregs in the treatment of autoimmune myositis (EAM) in mice and explore the possible mechanisms.</p><p><b>METHODS</b>Mouse models of EAM were divided randomly into model group and treatment group, and the latter received infusion of CD4(+) CD25(+) Foxp3(+) Tregs separated from normal mouse spleen by magnetic activated cell sorting. The changes of muscle pathology was observed, and the expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs was analyzed using flow cytometry; peripheral blood IL-10 and TGF-β levels were tested using double antibody sandwich ELISA.</p><p><b>RESULTS</b>Compare with the model group, the mice in the treatment group showed significantly reduced muscular inflammatory cell infiltration, increased blood levels of IL-10 and TGF-β (P<0.05), and increased expression of PD-1 and CTLA-4 in spleen CD4(+) CD25(+) Foxp3(+) Tregs (P<0.05).</p><p><b>CONCLUSION</b>CD4(+) CD25(+) Foxp3(+) Tregs reinfusion produces therapeutic effect in mice with EAM by increasing peripheral blood IL-10 and TGF-β levels and PD-1 and CTLA-4 expressions in spleen CD4(+) CD25(+) Foxp3(+) Tregs.</p>


Subject(s)
Animals , Autoimmune Diseases , Allergy and Immunology , CTLA-4 Antigen , Metabolism , Cell Separation , Cell- and Tissue-Based Therapy , Disease Models, Animal , Flow Cytometry , Interleukin-10 , Blood , Mice , Myositis , Allergy and Immunology , Programmed Cell Death 1 Receptor , Metabolism , Spleen , Allergy and Immunology , T-Lymphocytes, Regulatory , Allergy and Immunology , Transforming Growth Factor beta1 , Blood
17.
Journal of Pharmaceutical Practice ; (6): 525-528,535, 2015.
Article in Chinese | WPRIM | ID: wpr-790530

ABSTRACT

Objective To design and synthesize novel Danshensu derivatives in order to improve their stability and cardio-protective effect against myocardial ischemia .Methods Novel Danshensu derivatives were synthesized chiefly viaesterification , methoxy phenol hydroxyl protection ,nitration and amine solution reaction .All the target compounds were evaluated their bio-logical effects on anti-myocardial ischemia with H9c2 cells in vitro .Results 15 compounds were synthesized and further con-firmed by 1 H NMR and MS .Pharmacological studies showed that few synthetic derivatives at 5μmol/L performed a higher bi-ological effect than Danshensu .Conclusion Most derivatives didn't show the best activity at the chosen concentration gradient that a further pharmacological trial need to be done .

18.
Journal of Pharmaceutical Practice ; (6): 389-391,405, 2015.
Article in Chinese | WPRIM | ID: wpr-790493

ABSTRACT

Danshensu is one of the main water soluble active ingredients of Salvia miltiorrhiza ,which has many phar-macological activities ,especially in cardiovascular system .T he cardiovascular pharmacological mechanism of Danshensu and its derivatives from the view of anti-apoptosis ,antioxidant ,inhibiting calcium overload and role in the inflammatory reaction path-ways etc .were summarized in this article .

19.
Article in Chinese | WPRIM | ID: wpr-815198

ABSTRACT

OBJECTIVE@#Astragaloside is a simple substance of saponin and the active constituent of astragali. It was reported that the astragaloside exerted therapeutical eff ect on viral myocarditis and dilated cardiomyopathy. The purpose of this study was to investigate the effect of astragaloside on TL1A expression in viral myocarditis.@*METHODS@#A total of 100 BALB/c mice were randomly divided into 6 groups: the normal control group (group A, n=10), the high-dose control group (group B, n=10), the myocarditis control group (group C, n=20), the low-dose group (group D, n=20), the middle-dose group (group E, n=20) and the high-dose group (group F, n=20). Mice in group A and group B were injected intraperitoneally with 0.1 mL EMEM solution, while mice in group C, D, E and F were treated with 0.1 mL of 1×102 TCID50 CVB3 (diluted in EMEM). Then, mice in group A and group B were treated with carboxymethycellulose solution and 9% astragaloside for 1 week, respectively. At the same time, mice in group C, D, E and F were treated with sodium carboxymethycellulose solution, 1% [0.07 g/(kg.d)], 3% [0.2 g/(kg.d)] and 9%[0.6 g/(kg.d)] astragaloside for 1 week, respectively. After 14 days, the mice were sacrificed and their hearts were collected. The expression levels of TL1A mRNA and protein in the myocardium were examined by RT-PCR and immunohistochemistry, respectively.@*RESULTS@#There was no death in the group A and B. The mortality in the group C, D, E and F was 45% (9/20), 30% (6/20), 25% (5/20) and 10% (2/20), respectively. Compared with the group C, the mortality in the group F was significantly decreased (P0.05). There was no any pathological lesion in the group A and B. The TL1A mRNA and protein expression in the myocardium of mice in the group A and B was at low level, with no difference between them (P>0.05). Compared with the group A, the expression levels of TL1A mRNA and protein in the group C were markedly up-regulated (P0.05).@*CONCLUSION@#Astragaloside may play a pivotal role in protection of the heart injury in viral myocarditis by suppressing the expression of TL1A.


Subject(s)
Acute Disease , Animals , Cardiomyopathy, Dilated , Drug Therapy , Coxsackievirus Infections , Drug Therapy , Drugs, Chinese Herbal , Pharmacology , Mice , Mice, Inbred BALB C , Myocarditis , Drug Therapy , Virology , Myocardium , Metabolism , Pathology , RNA, Messenger , Saponins , Pharmacology , Tumor Necrosis Factor Ligand Superfamily Member 15 , Metabolism , Up-Regulation
20.
China Pharmacy ; (12): 3238-3240, 2015.
Article in Chinese | WPRIM | ID: wpr-500997

ABSTRACT

OBJECTIVE:To evaluate therapeutic efficacy and ADR of amphotericin B in the treatment of AIDS combined with fungal infections retrospectively. METHODS:The cases of amphotericin B in the treatment of AIDS combined with fungal in-fections were collected our hospital. Total therapeutic efficacy,distribution of pathogenic bacteria,clearance rate and ADR were evaluated. RESULTS:89 patients were involved totally,among which 45 cases were marked effect,32 cases improved and 12 cas-es failed,with effective rate of 86.5%. The fungus were found or cultured in 76 cases,among which 64 strains were cleared,but 12 strains were not,with total clearance rate of 84.2%. There were 41 ADR cases(46.1%),while all completed the treatment ex-cept 2 developing severe renal failure(2.2%). CONCLUSIONS:Domestic amphotericin B could effectively treat AIDS complicat-ing with fungal infections,and the ADRs were acceptable.

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