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1.
Article in Chinese | WPRIM | ID: wpr-1022050

ABSTRACT

BACKGROUND:The in vitro construction of intestinal smooth muscle layer,as an important component of the intestinal wall,has attracted much attention in the bionic construction of tissue-engineered intestinal canal. OBJECTIVE:To explore the effects of cyclic mechanical stretching on the growth activity of intestinal smooth muscle cells and the expression of functional genes within collagen strips. METHODS:The collagen band culture system of intestinal smooth muscle cells was constructed using a self-designed collagen strip stretching culture device with self-made rat tail collagen as a scaffold and primary rat intestinal smooth muscle cells as seed cells.EthD-1/Calcein-AM cell activity staining,magenta staining,cytoskeleton-Ki67 immunofluorescence staining were used to observe the growth activity and proliferation of the cells,and quantitative RT-PCR was used to detect the expression of desmin,α-sma,and vimentin functional genes. RESULTS AND CONCLUSION:The collagen band culture system of intestinal smooth muscle cells was successfully constructed,and intestinal smooth muscle cells in the band had good cell activity.The number of Ki67 positive cells increased and desmin,α-sma and vimentin were significantly overexpressed under cyclic stretching and dynamic culture conditions(P<0.001).To conclude,mechanical stimulation is beneficial to maintain the growth phenotype of smooth muscle cells and promote their functional differentiation during three-dimensional culture in vitro.

2.
Article in Chinese | WPRIM | ID: wpr-1025589

ABSTRACT

Objective:To explore the latent classes of health behavior and explore the predictive factors among stroke patients.Methods:A total of 1 250 participants were recruited using cluster random sampling in September 2022. The general information, the modified Rankin scale(mRS), stroke prevention knowledge questionnaire(SPKQ), health behavior scale for stroke patients (HBS-SP), and short form-health belief model scale (SF-HBMS) were administered in the cross-sectional survey. Mplus 8.3 software was used to conduct a latent class analysis (LCA) on the health behavior of stroke patients, and SPSS 27.0 software was used to carry out multinomial Logistic regression to analyze the predictive factors of different latent classes of health behavior of stroke patients.Results:The health behavior of stroke patients obtained three latent classes: low health behaviors-lack of health responsibility group (66.9%, n=794), moderate health behaviors-poor compliance group (11.9%, n=141), and good health behaviors-insufficient exercise group (21.2%, n=251). Compared with good health behaviors-insufficient exercise group, stroke patients with shorter duration education time ( B=-0.589, OR=0.555, P=0.036), hemorrhagic stroke ( B=0.082, OR=1.086, P<0.001), fewer comorbidities ( B=-0.022, OR=0.978, P=0.026), higher mRS score ( B=-0.046, OR=1.047, P=0.004), lower SPKQ score ( B=-0.055, OR=0.947, P=0.016), and lower SF-HBMS score ( B=-0.085, OR=0.919, P<0.001) were more likely to be included in moderate health behaviors-poor compliance group. However, stroke patients with shorter duration education time ( B=-0.026, OR=0.974, P=0.003), rural areas dwelling ( B=0.800, OR=2.225, P=0.004), fewer comorbidities ( B=-0.056, OR=0.945, P<0.001), lower SPKQ score ( B=-0.101, OR=0.904, P<0.001), and lower SF-HBMS score ( B=-0.071, OR=0.931, P<0.001) were more likely to be included in low health behaviors-lack of health responsibility group. Conclusion:The health behavior of stroke patients has three latent classes. A targeted intervention should be carried out according to the characteristics of different classes to improve their health behavior levels.

3.
Article in Chinese | WPRIM | ID: wpr-1025590

ABSTRACT

Objective:To explore the potential facilitators and barriers to healthy behavior among stroke patients.Methods:Semi-structured interviews were conducted among 16 stroke patients from September 2022 to March 2023 using an objective sampling method.The interview guide was developed using the theoretical domain framework(TDF). Interviews were transcribed and refined the theme using directed content and induction analysis.Using the TDF as the initial coding framework, the themes were then merged into the most relevant domains.Finally, the correspondence between theoretical domains and the healthy behavior of stroke patients was determined based on the frequency and relationship between themes.Results:This study identified nine theoretical domains that affected the healthy behavior of stroke patients: knowledge, skills, motivation and goals, social influences, social/professional role and identity, environment context and resources, belief about capability, consequence belief and behavioral regulation.Conclusion:The healthy behavior of stroke patients is complex and influenced by several factors.The nine theoretical domains identified in this study will provide recommendations for future healthy behavior interventions for stroke patients.

4.
Article in Chinese | WPRIM | ID: wpr-1025605

ABSTRACT

Objective:To explore the anxiety level, influencing factors among surrogate decision-makers of patients with acute ischemic stroke during thrombolysis decision-making, and their correlation with decision-making duration.Methods:Acute ischemic stroke patients and their surrogate decision-makers who visited the Emergency Department of the First Affiliated Hospital of Zhengzhou University from September 2019 to December 2021 were selected as the research subjects.Sociodemographic data and disease related data of patients and surrogate decision-makers were collected.Surrogate decision-makers were evaluated with the state-trait anxiety inventory, decision participation expectation scale, Wake Forest physician trust scale, and perceived social support scale.SPSS 26.0 software was used for data processing.Pearson correlation analysis, Spearman correlation analysis and ridge regression analysis were used for statistical analysis.Results:The score of state anxiety of decision-makers was (49.47±9.04), and 18.2% (70/383) of decision-makers had a decision duration exceeding 15 minutes.The score of state anxiety of decision-makers was positively correlated with decision duration ( r=0.189, P<0.001). The influencing factors of state anxiety level of decision-makers included sociodemographic factors (age of decision-makers and patients, relationship between payers and patients, whether decision-makers bear the current medical expenses, type of medical insurance for patients), psychological factors (trust level in physicians, perceived social support), factors related to patient disease (numbers of stroke relapses, National Institutes of Health stroke scale scores for patients), characteristics of the decision-making process (whether patients participate in the decision-making process, and the role of decision-makers in the decision-making process) (all P<0.05). Conclusion:Most surrogate decision-makers experience anxiety.Medical staff should pay attention to the emotions of decision-makers and adopt appropriate communication skills when communicating with informed consent for thrombolysis, alleviate the anxiety of surrogate decision-makers, so as so reduce the decision-making duration.

5.
Article in Chinese | WPRIM | ID: wpr-1027954

ABSTRACT

Objective:To construct an evaluation indicator system for the health management effects in high-risk stroke population.Methods:From March to May 2020, based on health ecology theory, social cognitive theory and knowledge, attitude/belief, practice theory, the first draft of the evaluation index system for health management effects of high-risk stroke groups was drawn up by literature review and group discussion. Through two rounds of Delphi expert consultation, the evaluation index system of health management effects of high-risk stroke groups was established, and the weight of each index was determined by analytic hierarchy process (AHP). A total of 22 experts were invited to participate in expert consultation. Twenty-two questionnaires were sent out in the first round, and 20 questionnaires were recovered, of which 20 were valid (90.9%). In the second round of correspondence, 20 questionnaires were sent out and 19 questionnaires were recovered, of which 19 were valid (95.0%).Results:The authority coefficients of the two rounds of expert consultation (Cr) was 0.933 and 0.937, respectively. The Kendall coordination coefficients W of importance in the second round was significantly higher than that in the first round (0.299 vs 0.172) ( P<0.001). The mean of importance score (Mj) of each index was 4.10-5.00, coefficient of variation (CV) was 0-0.235, and full mark ratio (Kj) was 0.26-1.00. Finally, an evaluation index system of the health management effects for high-risk stroke population was constructed, which included 3 first-level indicators (individual characteristics, behavior style, environmental support), 12 second-level indicators and 58 third-level indicators. Conclusions:The evaluation index system of the health management effects for high-risk stroke population is established in this study, which provides scientific quantitative indicators and evaluation tools. The enthusiasm, authority and coordination of consultation experts are strong, which indicates that the indicator system is feasible.

6.
Article in Chinese | WPRIM | ID: wpr-1036428

ABSTRACT

Objective @#To construct a high⁃throughput in vitro method for the detection of botulinum toxin A enzyme activity by using fluorescence resonance energy transfer(FRET) .@*Methods@# Recombinant expression plasmid based on double fluorescent labeled substrate was constructed to recognize botulinum toxin type A only , and the constructed plasmid was transferred into the E. coli expression system for expression. The expressed recombinant protein of fluorescent labeled substrate was purified , dialyzed and stored for standby; The activity of the recombinant proteinwas detected by digestion of botul inum toxin type A light chain ( ALc) ; The conditions of this detection method were optimized ; The enzyme kinetic parameters K m and K cat were determined by cutting the fluorescent labeled substrate with ALc. @*Results @#The recombinant expression plasmid was successfully constructed. After being expressed in the E. coli expression system , the target band appeared obviously. The purity of the purified recombinant protein was about 90% . The recombinant protein was named CYA. CYA was identified by enzyme digestion of ALc and Botulinum toxin type B light chain (BLc) . The results showed that CYA could only be digested by ALc to produce two protein fragments that were consistent with expectations , but could not be digested by BLc. By optimizing the conditions based on FRET substrate , it was obtained that the filter sensitivity was set between 65 - 110 ; The realtime dynamic detection interval was 2 min/time , the dynamic detection time was 30 - 120 min , and the appropriate concentration range of substrate CYA was 0. 5 - 32 μmol/L. The ratio of the time change of CYA at any time under the action of ALc enzyme to the fluorescence value 528 and 485 was plotted to be about 0. 5 at the minimum and 0. 9 at the maximum. The enzyme kinetic parameters determined that the value of ALC cleaving CYA K cat was (5 ±0. 4) s - 1 and K m was (2. 33 ± 0. 21) μmol/L.@* Conclusion@#A high⁃throughput in vitro method for the detection of botulinum toxin type A activity based on FRET technology is successfully constructed. Key words FRET;botulinum toxin;high throughput screening

7.
Acta Pharmaceutica Sinica B ; (6): 5074-5090, 2023.
Article in English | WPRIM | ID: wpr-1011205

ABSTRACT

Autologous cancer vaccine that stimulates tumor-specific immune responses for personalized immunotherapy holds great potential for tumor therapy. However, its efficacy is still suboptimal due to the immunosuppressive tumor microenvironment (ITM). Here, we report a new type of bacteria-based autologous cancer vaccine by employing calcium carbonate (CaCO3) biomineralized Salmonella (Sal) as an in-situ cancer vaccine producer and systematical ITM regulator. CaCO3 can be facilely coated on the Sal surface with calcium ionophore A23187 co-loading, and such biomineralization did not affect the bioactivities of the bacteria. Upon intratumoral accumulation, the CaCO3 shell was decomposed at an acidic microenvironment to attenuate tumor acidity, accompanied by the release of Sal and Ca2+/A23187. Specifically, Sal served as a cancer vaccine producer by inducing cancer cells' immunogenic cell death (ICD) and promoting the gap junction formation between tumor cells and dendritic cells (DCs) to promote antigen presentation. Ca2+, on the other hand, was internalized into various types of immune cells with the aid of A23187 and synergized with Sal to systematically regulate the immune system, including DCs maturation, macrophages polarization, and T cells activation. As a result, such bio-vaccine achieved remarkable efficacy against both primary and metastatic tumors by eliciting potent anti-tumor immunity with full biocompatibility. This work demonstrated the potential of bioengineered bacteria as bio-active vaccines for enhanced tumor immunotherapy.

8.
China Pharmacy ; (12): 465-472, 2022.
Article in Chinese | WPRIM | ID: wpr-920464

ABSTRACT

OBJECTIVE To study the metabolites of four diterpenoids of Euphorbia fischeriana in liver microsomes of rats and to investigate its metabolic regularity. METHODS In vitro incubation system of liver microsomes of rats was built. The jolkinolide A,jolkinolide B ,17-hydroxyl jolkinolide A and 17-hydroxyl jolkinolide B were added into incubation system of liver microsomes in rats activated by reduced nicotinamide adenine dinucleotide phosphate ,incubated at 37 ℃ for 30 min,and then terminated the reaction with acetonitrile. Taking the negative group (adding acetonitrile firstly and then starting incubation for 30 min)as the reference,the ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry was used ;Anaylyst®TF 1.7.1、PeakView® 2.2,MetabolitePilot 1.5 and MasterView 1.2 software were used to speculate and identify the fragmentation law of mass spectrometry and metabolites. RESULTS Four diterpenoids were easy to lose neutral fragments such as H 2O and CO in secondary mass spectrometry. Jolkinolide A and 17-hydroxyl jolkinolide A showed similar metabolism pathway ,including dihydroxylation,dehydrogenation,and monohydroxylation ;six and five metabolites were identified respectively. Jolkinolide B and 17-hydroxyl jolkinolide B showed similar metabolism pathway ,including monohydroxylation ,hydration and isomerization. Five metabolites were identified. CONCLUSIONS Both jolkinolide A and 17-hydroxyl jolkinolide A produce the metabolites of hydroxylation and dehydrogenation in liver microsomes of rats ;both jolkinolide B and 17-hydroxyl jolkinolide B produce the metabolites of hydroxylation ,hydration and isomerization in liver microsomes of rats. The metabolites of four diterpenoids are phase Ⅰ metabolites.

9.
Article in Chinese | WPRIM | ID: wpr-1038687

ABSTRACT

Objective @#To analyze interacting proteins of tropomodulin1 (TMOD1 ) in Raw264.7 mouse monocyte macrophage line by mass spectrometry and GeneCards database.@*Methods @#Immunoprecipitation combined with mass spectrometry was used to find interacting proteins of TMOD1 after overexpress TMOD1 in Raw264.7 cells. GeneCards database was used to search for known genes for macrophage migration.Bioinformatics & Systems Biolo- gy was used to analyze correlation between known targets and mass spectrometry proteins to find common differenti- ally expressed proteins( CO-DEPs) .WoLF PSORT was used to predict subcellular localization of CO-DEPs.Egg- NOG databasewas used to analyze eukaryotic orthologous group(KOG) of CO-DEPs.DAVID database was used to analyze gene ontology( GO) enrichment kyoto encyclopedia of genes and genomes( KEGG) pathway of CO-DEPs. String database was used to analyze protein interaction network and CytoScape software drawing. @*Results @#There were 41 CO-DEPs in mass spectrometry and GeneCards database.Subcellular localization of CO-DEPs was mainly distributed in cytoplasm,nucleus and mitochondria.KOG notes were mainly O : post-translational modification,Z : cytoskeleton and J : translation.GO enrichment found that CO-DEPs was mainly involved in poly (A) RNA bind- ing,protein folding and focal adhesion.KEGG was mainly enriched in arrhythmogenic right ventricular cardiomyop- athy (ARVC) and tight junction.ACTB was a protein with large protein interaction.@*Conclusion @#The proteins in- teracting with TMOD1 in macrophages mainly include myosin heavy chain-9 (MYH9) ,α-actinin 1 (ACTN1) and β-actin (ACTB) ,etc,suggesting that TMOD1 is related to macrophages migrate.

10.
China Pharmacy ; (12): 2868-2874, 2021.
Article in Chinese | WPRIM | ID: wpr-906653

ABSTRACT

OBJECTIVE:To study the improvement eff ects and p otential mechanism of chelidonine on CCl 4-induced hepatic fibrosis model rats. METHODS :According to the random number table method ,a total of 48 rats were randomly divided into normal control group ,model group ,chelidonine low-dose ,middle-dose and high-dose groups (0.125,0.25,0.50 mg/kg),positive control group (Liver-protecting tablet ,0.42 g/kg),with 8 rats in each group. Except for normal control group ,other groups were given CCl 4-olive oil solution intraperitoneally for 8 weeks to induce hepatic fibrosis model. From the fifth week of modeling , normal control group and model group were given water intragastrically ;administration groups were given relevant medicine intragastrically,once a day ,for consecutive 10 weeks. After last intragastric administration ,hepatic index of rats was calculated. The levels of aspartate aminotransferase (AST),alanine aminotransferase (ALT)and hyaluronic acid (HA)in serum and the level of hydroxyproline (Hyp)in liver tissue were determined. The staining of collagen fibrin in rat liver was observed. The protein expression of α-smooth muscle actin (α-SMA),microtubule-associated protein 1 light chain 3(LC3)and p 62 as well as the phosphorylation level of phosphoinositide 3 kinase(PI3K),protein kinase B (Akt)and mammalian target of rapamycin (mTOR)in liver tissue were determined ;mRNA expression corresponding to above protein were also determined. RESULTS :Compared with normal control group ,the hepatic index ,the serum levels of AST ,ALT,HA and Hyp ,the percentage of positive staining area for collagen fibrin ,the mRNA and protein expression of α-SMA and LC 3- Ⅱ were increased significantly (P<0.05). Protein expression of p 62,phosphorylation levels of PI 3K,Akt and mTOR as well as mRNA expression of p 62,PI3K,Akt and mTOR were significantly down-regulated (P<0.05). Compared with model group ,phosphorylation levels of PI 3K and mTOR were decreased significantly in chelidonine low-dose group (P<0.05). The changes of above indexes in chelidonine middle-dose and high-dose groups (except for liver index , HA level in middle-dose group ) were reversed significantly (P<0.05). CONCLUSIONS:Chelidonine can attenuate CCl 4-induced liver fibrosis in rats ;the mechanism of it may be associated with activating PI 3K/Akt/mTOR signaling pathway and inhibiting autophagy.

11.
Article in Chinese | WPRIM | ID: wpr-909516

ABSTRACT

Objective:To explore the reliability and validity of the community commitment scale (CCS) in community-dwelling elderly people.Methods:From May to August in 2020, a total of 526 community-dwelling elderly were investigated by community commitment scale, community's self-efficacy scale for preventing social isolation (CSES). SPSS 25.0 and AMOS 22.0 softwares were used to analyze the reliability and validity.Results:The overall Cronbach's α coefficient of the community commitment scale was 0.818, and the Cronbach's α coefficients of social and belonging dimensions were 0.750 and 0.777, respectively. The Guttman split-half reliability coefficients were 0.799-0.835, and the test-retest reliability was 0.802. Each item-to-total score correlations were 0.614-0.753 (all P<0.05). The item level content validity index (I-CVI) was 0.830-1.000, the scale level content validity index (S-CVI/UA) was 0.875, and the average content validity index (S-CVI/Ave) was 0.972. Two common factors were extracted by exploratory factor analysis, and the cumulative variance contribution rate was 57.019%. The confirmatory factor analysis all indicators reached the adaptation standard (χ 2/ df=2.638, RMSEA=0.079, GFI=0.957, AGFI=0.918, TLI=0.936, CFI=0.956, IFI=0.957), and standardized regression coefficients were 0.63-0.80. Conclusion:The Chinese version of the community commitment scale has good reliability and validity and can be used to evaluate the social isolation of the elderly in the community.

12.
Article in Chinese | WPRIM | ID: wpr-754124

ABSTRACT

Objective To evaluate the reliability and validity of the Chinese Version of the Tampa Scale of Kinesiophobia (TSK-11) in patients receiving total knee arthroplasty. Methods The Chinese ver-sion of TSK-11 was translated from the original one,and adjusted for cultural adaptation. The reliability and validity of Chinese TSK-11 were tested among 254 eligible patients receiving total knee arthroplasty from a tertiary hospital in Henan province by convenience sampling. Results Three common factors which ex-plained 65. 177% of the total variance was extracted after the exploratory factor analysis,and each item had high factor loading quantity (>0. 4). The scale CVI was 0. 83~1. 00,the S-CVI/UA and S-CVI/Ave were 0. 91 and 0. 94,respectively. The Cronbach's α coefficient of the scale was 0. 883,and the test-retest reliabil-ity was 0. 798. The item-to-total correlations ranged from 0. 424~0. 757 (P<0. 05). Conclusion The Chi-nese version of TSK-11 has been proved to be reliable and valid with fewer understandable items,and short completion time. It can be used as a valuable tool for evaluating kinesiophobia in patients following total knee arthroplasty.

13.
China Pharmacy ; (12): 2963-2967, 2019.
Article in Chinese | WPRIM | ID: wpr-817477

ABSTRACT

OBJECTIVE: To study the inhibitory effect of dictamnine on the viability of mice spleen lymphocyte in vitro and explore its potential mechanism. METHODS: The primary spleen lymphocytes of mice were isolated and cultured. The cells were treated with 0 (blank control), 50, 100, 150 μmol/L dictamnine for 24 h. MTT assay was used to determine the cell viability; Lactic dehydrogenase (LDH) assay was used to determine release rate of LDH. Early apoptosis rate was detected by flow cytometry. Necrosis rate was detected by Hoechst 33342 and PI double staining; Western blot assay was used to detect the protein expressions of Caspase 3 and Cleaved-Caspase 3 in cells. Comet assay was used to detect DNA damage in cells (reflected in the proportion of DNA tail area). RESULTS: Compared with blank control, 100, 150 μmol/L dictamnine could significantly inhibit the viability of lymphocytes (P<0.01). 150 μmol/L dictamnine could significantly increase the release of LDH (P<0.05), and release rate reached 79.37%. 50, 100, 150 μmol/L dictamnine could improve the early apoptotic rate of lymphocyte, but there was no statistical significance (P>0.05). 150 μmol/L dictamnine could significantly increase the necrosis rate (P<0.05), and necrosis rate reached 78.64%. 50, 100, 150 μmol/L dictamnine could increase the protein expression of Caspase 3, but there was no statistical significance (P>0.05), while 50, 100 μmol/L dictamnine could improve the protein expression of Cleaved-Caspase 3 significantly (P<0.05). DNA damage was induced in a dose-dependent manner by dictamnine, in which 100 and 150 μmol/L dictamnine could significantly increase DNA tail area (P<0.01). CONCLUSIONS: Dictamnine can inhibit spleen lymphocyte viability, and the mechanism may be related to inducing spleen lymphocyte necrosis and DNA damage.

14.
China Pharmacy ; (12): 2796-2801, 2019.
Article in Chinese | WPRIM | ID: wpr-817523

ABSTRACT

OBJECTIVE: To analyze the metabolites of jolkinolide B in rats, and predict its metabolism pathway. METHODS: The rats were randomly divided into blank group (0.5% CMC-Na, ig) and administration group (jolkinolide B, ig, 100 mg/kg), with 8 rats in each group. The fecal samples were collected at >0-12, >12-24, >24-36 hours after administration; the urine samples were collected at >0-2, >2-8, >8-12, >12-24, >24-36, >36-48 hours after administration; the blood samples were collected at 1, 2, 8, 12, 24, 36 hours after administration. UPLC-Q-TOF/MS combined with Analyst® TF 1.7.1 and PeakView® 2.2 software were used to analyze and identify the metabolites in the samples after treated with ultrasonic extraction, solid phase extraction and protein precipitation. RESULTS & CONCLUSIONS: Prototype drugs and seven metabolites were detected in rat’s fecal samples, and one or two metabolites were detected in urine and blood samples, respectively. After intragastric administration, the metabolism of jolkinolide B in rats is mainly through ring opening, oxidation, dehydration, deoxygenation and hydrogenation of phase Ⅰ, but no phase Ⅱ metabolites were detected.

15.
Article in Chinese | WPRIM | ID: wpr-744766

ABSTRACT

Objective To explore the consistency between the Chinese Version of Stressors in Nursing Students (SINS-CHI) and the Student Nurse Stress Index Scale (SNSI-CHI) in assessing the pressure of undergraduate nursing students.Methods A total of 960 nursing undergraduates from three universities in Henan province were randomly selected.SINS-CHI and SNSI-CHI were applied to all subjects and SPSS 21.0 was used to analyze the validity and reliability.Results The total score of SINS-CHI was (112.73± 26.07).The Cronbach's α was 0.926,the Guttman's partial reliability coefficient was 0.893,the test-retest reliability was 0.820,the content validity index (CVI) was 0.890,the sensitivity was 70.0%,and the specificity was 62.3%.The Yoden index was 0.323 and the area under the ROC curve was 0.710.The total score of SNSI-CHI was (58.71± 14.18).The Cronbach's α was 0.893,Guttman split-half reliability coefficient was 0.829,test-retest reliability was 0.966,CVI was 0.954,sensitivity was 71.4%,specificity was 63.6%.The Yoden index was 0.350 and the area under the ROC curve was 0.714.Conclusion The consistency of SINS-CHI and SNSI-CHI is good.

16.
Article in Chinese | WPRIM | ID: wpr-753858

ABSTRACT

Objective To investigate the antimicrobial resistance of clinical bacterial isolates in Peking Union Medical College Hospital (PUMCH) in 2017. Methods A total of 9 515 non-duplicate clinical isolates were collected from January 1 to December 31, 2017. Disc diffusion test (Kirby-Bauer method) and E-test method were employed to determine antimicrobial susceptibility. Results Gram-negative bacilli and gram-positive cocci accounted for 68.2% and 31.8%, respectively among the 9 515 clinical isolates. Methicillin-resistant strains in S. aureus (MRSA) and coagulase-negative Staphylococcus (MRCNS) accounted for 25.6% and 73.3%, respectively. Extended-spectrum β-lactamases (ESBLs) -producing strains accounted for 47.6% (877/1 842), 27.6% (335/1 213) and 33.0% (59/179) in E. coli, Klebsiella spp (K. pneumoniae and K. oxytoca) and P. mirabilis, respectively. Enterbacteriaceae strains were still highly susceptible to carbapenems, with an overall resistance rate of ≤ 3.8%. The resistance rates of K. pneumoniae to imipenem and meropenem were 8.5% and 8.2%, respectively. About 72.7% and 70.4% of A. baumannii isolateswere resistant to imipenem and meropenem. The resistance rate of P. aeruginosa to imipenem and meropenem was 14.8% and 10.0%, respectively. The prevalence of extensively drug-resistant strains in A. baumannii, P. aeruginosa and K. pneumoniae was 31.7% (239/753), 1.0% (10/1 035), and 3.0% (33/1 117), respectively. Conclusions The common bacterialisolates show various level of resistance to antimicrobial agents. Laboratory staff should improve communication with clinicians to prevent the spread of resistant strains.

17.
Article in Chinese | WPRIM | ID: wpr-806849

ABSTRACT

Objective@#To evaluate the performance of Vitek MS, an automated mass spectrometry microbial identification system, in identification of clinical filamentous fungi isolates, which provide a new insight into filamentous fungi identification strategy.@*Methods@#Twenty-five isolates of filamentous fungi genetically confirmed by molecular sequencing method were inoculated in different culture conditions to analyze the potential influences of culture conditions on the identification of filamentous fungi with Vitek MS. Further evaluation of the performance of Vitek MS in filamentous fungi identification was carried out with 207 clinical isolates of filamentous fungi and the comparison of the results with those of morphology and sequencing analysis.@*Results@#The performance of Vitek MS in filamentous fungi identification was not significantly influenced by culture conditions. Vitek MS successfully identified 87.9% (182/207) of all tested filamentous fungi. Lacking of reference MS spectra in Vitek database was the main reason that the other isolates could not be identified. Vitek MS was superior to the traditional morphological method in identification of filamentous fungi especially non-Aspergillus molds at species level.@*Conclusion@#Vitek MS is an efficient approach for clinical filamentous fungi identification. Continuous enrichment of the species coverage in database will be of great importance for improving the performance of Vitek MS in identification of filamentous fungi.

18.
Article in Chinese | WPRIM | ID: wpr-704171

ABSTRACT

Objective To test the agreement between two new self-care-related instruments,the Chinese version of the Appraisal of Self-care Agency Scale-Revised (ASAS-R-CHI) and the Chinese version of the Self-care Ability Scale for the Elderly (SASE-CHI) among older stroke patients by Bland-Altman method.Methods A random sampling was used to conduct this study in Neurology Department in a tertiary referral hospital in Henan Province,during November 2016 to October 2017.The sample consisted of 500 older stroke patients.Data were collected by a demographic questionnaire,SASE-CHI and ASAS-R-CHI.SPSS 21.0 was used to analyze the data.Results The mean total scores of ASAS-R-CHI and SASE-CHI were 54.98±5.06 and 61.92±7.34,respectively.Partial correlation coefficients between the total score of ASAS-R-CHI and SASE-CHI and their factors were from 0.217 to 0.953,showed significantly correlation (P<0.01).The difference analysis and ratio analysis of Bland-Altman showed a good consistency of the two scales,with 5.0% and 4.5% of the points outside the 95%CI boundaries,separately.Conclusion There is a good agreement between the two instruments among older stroke patients.Each scale has its own characteristics,therefore the users should choose the most appropriate scale according to the specific situation.

19.
Article in Chinese | WPRIM | ID: wpr-704188

ABSTRACT

Objective To test the reliability and validity of the Chinese Version of the Student Nurse Stress Index Scale ( SNSI-CHI ) among Chinese nursing students. Methods A random sampling method was used to collect 1100 nursing students who were from two medical universities of Henan Province, China,and SPSS 17. 0 and AMOS 17. 0 software were used. Results The average total score of SNSI-CHI was 58. 46±13. 90. The Cronbach's α was 0. 886,the test-retest intra-class correlation coefficient( ICC) of SNSI-CHI was 0. 996 (95%CI:0. 992-1. 000,P<0. 01). The item-to-total correlations ranged from 0. 351 to 0. 664 ( all P<0. 01) . The content validity index( CVI) was 0. 954. The result of exploratory factor analysis ( EFA) was that three factors together explained 75. 013% of the total variances,and the confirmatory factory analysis( CFA) also indicated a good fit (χ2/df=1. 347,GFI=0. 956,AGFI=0. 945,RMR=0. 032,RMSEA= 0. 025, NFI=0. 974, IFI=0. 993, TLI=0. 992, CFI=0. 993 ) . Criterion-related validity was between 0. 330 and 0. 903 ( all P<0. 01) . Conclusion The SNSI-CHI is proved to be reliable and valid in China, and it can be used to measure the stress of Chinese nursing students.

20.
Chongqing Medicine ; (36): 3536-3538,3541, 2017.
Article in Chinese | WPRIM | ID: wpr-606939

ABSTRACT

Objective To adopt the cone beam computed tomography(CBCT) to analyze the bone thickness of infrazygomatic crest with different vertical skeletal facial types in teenagers.Methods Sixty teenagers of skeletal class were collected,including each 20 cases of high angle,average angle and low angle.The CBCT scanning data of oral maxillofacial region were collected.The bone thickness at different coronary slices in buccal side of infrazygomatic crest region at 13,15,17 mm above the maxillary occlusal plane was measured.The measured data were analyzed statistically.Results The bone thickness of infrazygomatic crest was gradually thinned from down to up,and the difference was statistically significant(P<0.05);the bone thickness(except 3 points at 13 mm from maxillary plane) at each measured point in the high,average and low angle groups showed the change trend of low angle >average angle>high angle,the difference was not statistically significant(P>0.05).Conclusion In teenagers,the bone thickness of infrazygomatic crest in skeletal class Ⅱ is gradually thinned from cranial direction and rearward,the high angle is thinnest and the low angle is thickest.

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