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Objective:To analyze the cause of infusion timeout of 5-fluorouracil (5-FU) powder injection infusion pumps, and conduct research on the dispensing methods, then provide a basis for clinical dispensing.Methods:The dissolution effect of 5-FU in different solvents were tested in the lab. The effect of different solvents on infusion timeout of infusion pumps, and the factors related to infusion timeout of the pumps were explored by analyzing the clinical data which was collected in Cancer Hospital of Shantou University Medical College from May 20 to July 20, 2020.Results:The dissolving capacity to 5-FU of different solvents sorted by the influence in a descending manner as follows: water for injection> 5% glucose injection (5%GS) >0.9% sodium chloride injection (0.9%NS) . Infusion timeout value of water for injection group (15.03 ± 8.62)% was lower than that of 0.9%NS group (36.78 ± 4.81)%, (0.9%NS+ water for injection) group (22.50 ± 7.22)%, 5%GS group (25.53 ± 6.21)% and (5%GS+ water for injection) group (24.78 ± 4.36)% ( t values were 2.50-5.27, all P<0.05). The timeout value of 0.9%NS group was higher than that of other groups ( t values were 3.65-5.27, all P<0.05). There were differences in infusion timeout between intravenous infusion group (23.07 ± 8.98)% and arterial infusion groups (60.60 ± 58.64)% ( H=10.18, P=0.001). There was a positive correlation between drug concentration and infusion timeout( r=0.29, P=0.013), and a negative correlation between total liquid volume and infusion timeout ( r=-0.59, P<0.01). Infusion timeout of pumps was partly (67.3%) affected by drug concentration, total liquid volume and infusion route. Conclusions:The research shows that infusion timeout of 5-FU powder injection infusion pumps is related to drug concentration, total liquid volume and infusion route. It is suggested that the percentage of water for injection, drug concentration, total liquid volume, and infusion route should be considered when 5-FU powder injection infusion pumps are prepared.
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OBJECTIVE To analyze th e evaluation methods of the skill ，adherence and effectiveness of medication in bronchial asthma （hereinafter referred to as “asthma”）patients from a pharmacist perspective ，and to provide reference for pharmacists to carry out clinical intervention researches and management of asthma patients. METHODS Referring to diagnosis and treatment guidelines ，evidence-based medicine literature and clinical practice experience ，the evaluation methods of asthma patients ’ medication with clinical value were expounded from three aspects including medication skill （inhalation technology ），adherence and effectiveness. RESULTS Inhalation technique rating form was an important evaluation method of medication skills in asthma patients. Medication adherence could be evaluated by Morisky Medication Adherence Scale or the Medication Adherence Report Scale for Asthma. Effectiveness evaluation methods contained direct evaluation indexes （such as clinical manifestations ，situation of acute exacerbation and relieving medication ，examination indexes such as pulmonary function indexes and fractional concentration of exhaled nitric oxide ）and indirect evaluation tools （i.e. various scales ，including Asthma Control Test ，Children-Asthma Control Test，the Test for Respiratory and Asthma Control in Kids ，asthma related quality of life scales ，etc.）CONCLUSIONS The combination of the evaluation methods of medication skill ， adherence and effectiveness contributes to assessing the pharmacotherapy effect of asthma patients. Pharmacists should apply and perfect these evaluation methods in practice ，so as to conduct better pharmaceutical intervention on asthma patients.
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OBJECTIVE@#To clarify the functional effects of differential expression of ring finger and tryptophan-aspartic acid 2 (RFWD2) on dendritic development and formation of dendritic spines in cerebral cortex neurons of mice.@*METHODS@#Immunofluorescent staining was used to identify the location and global expression profile of RFWD2 in mouse brain and determine the co-localization of RFWD2 with the synaptic proteins in the cortical neurons. We also examined the effects of RFWD2 over-expression (RFWD2-Myc) and RFWD2 knockdown (RFWD2-shRNA) on dendritic development, dendritic spine formation and synaptic function in cultured cortical neurons.@*RESULTS@#RFWD2 is highly expressed in the cerebral cortex and hippocampus of mice, and its expression level was positively correlated with the development of cerebral cortex neurons and dendrites. RFWD2 expression was detected on the presynaptic membrane and postsynaptic membrane of the neurons, and its expression levels were positively correlated with the length, number of branches and complexity of the dendrites. In cultured cortical neurons, RFWD2 overexpression significantly lowered the expressions of the synaptic proteins synaptophysin (P < 0.01) and postsynapic density protein 95 (P < 0.01), while RFWD2 knockdown significantly increased their expressions (both P < 0.05). Compared with the control and RFWD2-overexpressing cells, the neurons with RFWD2 knockdown showed significantly reduced number of dendritic spines (both P < 0.05).@*CONCLUSION@#RFWD2 can regulate the expression of the synaptic proteins, the development of the dendrites, the formation of the dendritic spines and synaptic function in mouse cerebral cortex neurons through ubiquitination of Pea3 family members and c-Jun, which may serve as potential treatment targets for neurological diseases.
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Animals , Aspartic Acid/metabolism , Cerebral Cortex , Dendritic Spines/metabolism , Mice , Neurons/metabolism , Synapses , Tryptophan/metabolismABSTRACT
Objective: To analyze the repetitive reporting of hepatitis B in Fujian province during 2016-2020, and provide evidence for the improvement of hepatitis B surveillance. Methods: The reporting cards from the China Information System for Disease Control and Prevention were collected and divided into repetitive reporting cards and non-repetitive reporting cards from the report cards collected according to the valid ID number on the cards, and the proportion of repetitive report cards and related factors were analyzed by using software SAS 9.4. Results: A total of 314 551 hepatitis B reporting cards were submitted in Fujian from 2016 to 2020, in which 90.93% (286 020/314 551) were included in the analysis. The repetitive reporting cards accounted for 10.48% (29 982/286 020). The annual proportion of the repetitive reporting cards from 2016 to 2020 was between 2.98% and 3.71%, showing an overall increasing trend year by year (Z=2.26, P=0.024). The proportions of the repetitive reporting cards in 1-5 years were 3.17%, 5.40%, 7.74%, 9.27% and 10.48%, respectively, showing an increase trend with year (Z=128.16, P<0.001). The proportions of the repetitive reporting cards in 10 areas of Fujian ranged from 5.44% to 13.48% with significant difference (χ2=2 050.41, P<0.001) and increased with the increase of reported incidence of hepatitis B (Z=26.92, P<0.001). There were significant differences in relationships between repetitive reporting proportion and sex, age and type of the cases between the areas with high incidence and low incidence of hepatitis B. Conclusions: The reported incidence of hepatitis B was seriously affected by the repetitive reporting in Fujian from 2016 to 2020. A cross-year and cross-area surveillance mechanism for hepatitis B should be established and targeted measures should be taken to strengthen the control of the repetitive reporting and improve the surveillance for hepatitis B.
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China/epidemiology , Data Collection , Hepatitis B/epidemiology , Humans , Incidence , SoftwareABSTRACT
Paeoniae Radix Rubra is a traditional Chinese medicine commonly used in clinical practice， it is mostly wild and widely distributed in different areas of China. In addition， the plant of Paeoniae Radix Rubra also has ornamental value. Modern phytochemical researches showed that the chemical constituents of Paeoniae Radix Rubra were complex. Up to now， more than 300 chemical constituents have been found， mainly including monoterpene glycosides， triterpenoids， flavonoids， tannins， phenolic acids， saccharides， steroids， volatile oils and so on. Among them， the content of monoterpene glycosides was the highest， and the types of volatile oil were the most. Paeoniae Radix Rubra has a wide range of pharmacological effects， exerting different curative effects in multiple systems such as blood， cardiovascular， nervous and digestive system. It can protect myocardial cells and nerve cells， stabilize microcirculation， anti-endotoxin， anti-atherosclerosis， reduce pulmonary hypertension， anti-depression， protect liver， anti-gastric ulcer， anti-tumor， slow down aging， treat Parkinson's syndrome and diabetes and its complications， anti-radiation， anti-inflammatory， anti-virus and so on. Through reviewing the literature on chemical constituents and pharmacological effects of Paeoniae Radix Rubra， it was found that total glycosides and monomers such as paeoniflorin， albiflorin， benzoylpaeoniflorin and gallic acid may be the main active components of Paeoniae Radix Rubra. At present， the research on Paeoniae Radix Rubra mainly focused on monoterpene glycosides， while the research on flavonoids and volatile oil in Paeoniae Radix Rubra was less. It is suggested that research on these two components should be strengthened in the future.
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@#AIM:To systematically compare and evaluate the clinical efficacy of intraocular lens(IOL)incarceration and non-incarceration in pediatric cataract.<p>METHODS: Literatures were searched from domestic and foreign databases such as PubMed, Embase, Cochrane Library, Wanfang, and CNKI, and the paper editions of relevant journals were consulted as well. The retrieval period of literature was from January 2000 to January 2021. The screened literatures were evaluated and extracted by two experienced researchers. After performing the evaluation guidelines of Cochrane collaboration and the Newcastle-Ottawa Scale(NOS), the Rev Man 5.4 software was applicated to complete the Meta-analysis.<p>RESULTS:Seven references(328 eyes)were involved in this analysis. The results of the Meta-analysis showed that the two groups had statistically significant differences in best corrected visual acuity(BCVA)>0.5 eyes(<i>RR</i>=2.00, 95%<i>CI</i>: 1.18-3.37, <i>P</i>=0.01), IOL shift(<i>RR</i>=0.28, 95%<i>CI</i>: 0.17-0.46, <i>P</i><0.00001)and mild or above opacification of the visual axis(<i>RR</i>=0.35, 95%<i>CI</i>: 0.19-0.65, <i>P</i>=0.0007)after surgery. However, there was no significant difference in the occurrence of posterior synechia(<i>RR</i>=0.67, 95%<i>CI</i>: 0.10-4.33, <i>P</i>=0.67)and very mild opacification of the visual axis(<i>RR</i>=1.05, 95%<i>CI</i>: 0.64-1.73, <i>P</i>=0.84).<p>CONCLUSION:IOL incarceration in the treatment of pediatric cataract can significantly improve postoperative BCVA, reduce occurrence of IOL shift and prevent mild or above opacification of the visual axis, which has more advantages in overall clinical efficacy. But more high quality prospective studies should be still required for further analysis.
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Objective: To explore the feature and influencing factors of anhedonia in schizophrenia. Methods: A total of 71 schizophrenia patients and 50 healthy controls were recruited during December 2018 and December 2019. Positive and Negative Syndrome Scale (PANSS) was used for assessing psychotic symptoms. Temporal Experience of Pleasure Scale (TEPS) and Repeatable Battery for the Assessment of Neuropsychological Status (RBANS) were applied to the evaluation of anhedonia and cognitive function respectively for each subject. Results: The evaluation result of TEPS showed both anticipatory and consummatory pleasure scores were lowered in patients with schizophrenia than those in healthy people (P=0.000). Stepwise linear regression analysis showed that negative subscale score of PANSS (B=-0.895, P=0.002) and delayed memory score of RBANS (B=0.265, P=0.001) were associated with anhedonia in schizophrenia. Conclusion: Patients with schizophrenia exhibit anticipatory and consummatory anhedonia, both of which are influenced by negative symptoms and delayed memory in schizophrenia.
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Intellectual development disorders are a group of etiologically diverse conditions originating during the developmental period characterized by significantly below average intellectual functioning and adaptive behavior. It is important to combine the personalized intervention with games and activities of daily living in community and family. Moreover, early intervention methods such as multi-sensory stimulation, activity observation training, goals-activity-motor enrichment are needed to improve their cognitive function; special education, intervention of behavioral, medicine and training of social skills, self-esteem and emotional quotient, to improve the outcome of children with intellectual development disorder.
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Radix Paeoniae Alba, one of the common Chinese medicinal materials, comes from the dried root of Paeonia lactiflora Pall, and mainly contains monoterpene glycosides, triterpenoids, flavonoids, tannins and other chemical constituents. At present, 140 chemical constituents have been isolated from Radix Paeoniae Alba, including 55 volatile oils, 33 monoterpenes and their glycosides, 13 flavonoids, 10 tannins, 9 organic acids, 9 triterpenes, 4 mixed terpenoids, 4 steroids and 3 phenylethanol glycosides. Modern pharmacological research has showed that Radix Paeoniae Alba has anti-inflammatory, liver protecting, analgesic, blood nourishing and other pharmacological effects. This paper summarizes the research results of chemical composition and pharmacological action of Radix Paeoniae Alba, in order to provide reference for expanding the clinical application and further development of Radix Paeoniae Alba.
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OBJECTIVE@#This study summarizes and compares clinical and laboratory characteristics of 34 patients admitted to the intensive care unit (ICU) for complications from coronavirus disease 2019 (COVID-19) at the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China from Jan. 22 to Mar. 5, 2020.@*METHODS@#A total of 34 patients were divided into two groups, including those who required noninvasive ventilation (NIV) and invasive mechanical ventilation (IMV) with additional extracorporeal membrane oxygenation (ECMO) in 11 patients. Clinical features of COVID-19 patients were described and the parameters of clinical characteristics between the two groups were compared.@*RESULTS@#The rates of the acute cardiac and kidney complications were higher in IMV cases than those in NIV cases. Most patients had lymphocytopenia on admission, with lymphocyte levels dropping progressively on the following days, and the more severe lymphopenia developed in the IMV group. In both groups, T lymphocyte counts were below typical lower limit norms compared to B lymphocytes. On admission, both groups had higher than expected amounts of plasma interleukin-6 (IL-6), which over time declined more in NIV patients. The prothrombin time was increased and the levels of platelet, hemoglobin, blood urea nitrogen (BUN), D-dimer, lactate dehydrogenase (LDH), and IL-6 were higher in IMV cases compared with NIV cases during hospitalization.@*CONCLUSIONS@#Data showed that the rates of complications, dynamics of lymphocytopenia, and changes in levels of platelet, hemoglobin, BUN, D-dimer, LDH and IL-6, and prothrombin time in these ICU patients were significantly different between IMV and NIV cases.
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Acute Kidney Injury , Virology , Aged , Aged, 80 and over , Betacoronavirus , Blood Urea Nitrogen , China , Coronavirus Infections , Therapeutics , Extracorporeal Membrane Oxygenation , Female , Fibrin Fibrinogen Degradation Products , Heart Diseases , Virology , Hemoglobins , Hospitalization , Humans , Intensive Care Units , Interleukin-6 , Blood , L-Lactate Dehydrogenase , Blood , Lymphopenia , Virology , Male , Middle Aged , Noninvasive Ventilation , Pandemics , Pneumonia, Viral , Therapeutics , Positive-Pressure Respiration , Prothrombin Time , Retrospective StudiesABSTRACT
Objective:To assess antimicrobial activity of Fe 3O 4 nanozymes against Candida albicans. Methods:Fe 3O 4 nanozymes were prepared by using a modified hydrothermal synthesis method. Candida albicans suspensions were divided into 4 groups: nanozyme group treated with 0.5 g/L Fe 3O 4 nanozymes, H 2O 2 group treated with 0.1% H 2O 2, combination group treated with 0.5 g/L Fe 3O 4 nanozymes and 0.1% H 2O 2, and control group receiving no treatment. Candida albicans in the above 4 groups was all cultured with Sabouraud liquid medium, the absorbance value at 600 nm was detected every 2 hours, and the growth of Candida albicans was observed. After 2-hour treatment, the morphology of Candida albicans in the 4 groups was observed by scanning electron microscopy; after plate coating, Candida albicans was cultured at 36 ℃ for 48 hours, colonies were observed and counted, and the inhibition rate of colony formation was calculated. One-way analysis of variance was used for comparing means among several groups, and least significant difference (LSD) - t test for multiple comparisons. Results:A relatively stable survival curve of Candida albicans was observed in the control group, while the growth of Candida albicans was inhibited in the nanozyme group, H 2O 2 group and combination group. The colony count significantly differed among the control group, H 2O 2 group, nanozyme group and combination group (124 830 ± 45 170, 86 330 ± 13 960, 91 670 ± 31 370 and 30 330 ± 3010 respectively; F = 9.41, P < 0.05) , and was significantly lower in the combination group than in the control group ( t = 4.63, P < 0.05) . There was a significant difference in the colony-formation inhibition rate among the H 2O 2 group, nanozyme group and combination group (30.84% ± 5.00%, 26.57% ± 11.24%, 75.70% ± 2.42% respectively; F = 9.413, P < 0.01) , and the combination group showed a significantly higher inhibition rate compared with the H 2O 2 group and nanozyme group ( t = 8.08, 4.27 respectively, both P < 0.01) . Scanning electron microscopy showed changes in the morphology of Candida albicans treated with H 2O 2 and nanozymes alone or in combination, including shrinkage, rupture and even collapse. Conclusion:Fe 3O 4 nanozymes combined with H 2O 2 have obvious antimicrobial effect against Candida albicans.
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Immunoglobulin G against myelin oligodendrocyte glycoprotein (MOG-IgG) is detectable in neuromyelitis optica spectrum disorder (NMOSD) without aquaporin-4 IgG (AQP4-IgG), but its pathogenicity remains unclear. In this study, we explored the pathogenic mechanisms of MOG-IgG in vitro and in vivo and compared them with those of AQP4-IgG. MOG-IgG-positive serum induced complement activation and cell death in human embryonic kidney (HEK)-293T cells transfected with human MOG. In C57BL/6 mice and Sprague-Dawley rats, MOG-IgG only caused lesions in the presence of complement. Interestingly, AQP4-IgG induced astroglial damage, while MOG-IgG mainly caused myelin loss. MOG-IgG also induced astrocyte damage in mouse brains in the presence of complement. Importantly, we also observed ultrastructural changes induced by MOG-IgG and AQP4-IgG. These findings suggest that MOG-IgG directly mediates cell death by activating complement in vitro and producing NMOSD-like lesions in vivo. AQP4-IgG directly targets astrocytes, while MOG-IgG mainly damages oligodendrocytes.
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Objective • To investigate the effects and potential mechanisms of fatty acid binding protein 3 (FABP3) on cell survival under hypoxia. Methods • Neonatal rat ventricular myocytes were stimulated with recombinant human FABP3 proteins, and then the differences of cell viability and cell death between groups were verified by trypan blue assay and MTT assay. Besides, the expression of PARP and caspase 3 protein, the level of reactive oxygen species (ROS), and mitochondrial membrane potentials under hypoxia were compared between groups for more confirmation. Results • FABP3 increased cardiomyocytes’ death and decreased cell viability under hypoxia (P=0.021). It was discovered that FABP3 upregulated the levels of cleaved PARP and cleaved caspase 3 (P=0.006, P=0.002), increased the level of intracellular ROS (P=0.038), and declined the mitochondrial membrane potentials as well (P=0.002). Conclusion • FABP3 contributes to cell survival and apoptosis by regulating intracellular ROS and mitochondrial membrane potentials under hypoxia.
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Depression is a common mental disorder and causes great disease burden. To date people's understanding of genetic mechanisms of depression is lagging far behind that of other psychiatric disorders such as schizophrenia and bipolar disorder. Recently, with the accumulation of clinical samples and the advances of methodology and technology, certain progress in genetics of depression has been made. This review summarizes the genetic research progress in candidate genes, common variants, rare variants and chromosome structure variations of depression.
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OBJECTIVE@#To study the clinical features, treatment, and prognosis of pure red cell aplasia (PRCA) in children.@*METHODS@#A retrospective analysis was performed for the clinical data of 16 children with PRCA. The outcome and prognosis of patients treated with prednisone combined with Huaiqihuang granules versus prednisone alone were evaluated.@*RESULTS@#All the 16 children complained of symptoms of anemia including pale or sallow complexion. Of 12 children undergoing pathogen test, 7 (58%) were found to have pathogen infection, among which human cytomegalovirus was the most common. Lymphocyte subsets were measured for 7 children, among whom 5 (71%) had lymphocyte immune disorder. Six children were found to have abnormalities in immunoglobulin and complement. The 8 children treated with prednisone combined with Huaiqihuang granules had a median follow-up time of 21.5 months, among whom 1 was almost cured, 1 was relieved, and 6 were obviously improved; the median onset time of treatment was 1 month, and 2 children had disease recurrence in the course of drug reduction or withdrawal. The 8 children in the prednisone alone treatment group had a median follow-up time of 34 months, among whom 4 were almost cured, and 4 were obviously improved; the median onset time of treatment was 2.5 months, and 4 children had recurrence during drug reduction or withdrawal.@*CONCLUSIONS@#Children with PRCA usually complain of anemia-related symptoms. Laboratory tests show pathogen infection in some children with PRCA, and most of children have immune disorders. Glucocorticoids have a good therapeutic effect, but some children relapse in the course of drug reduction or withdrawal. Combined treatment with prednisone and Huaiqihuang granules may have a faster onset of action and less possibility of recurrence.
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Child , Glucocorticoids , Humans , Prednisone , Recurrence , Red-Cell Aplasia, Pure , Retrospective StudiesABSTRACT
AIM:To observe the expression of hypoxia-inducible factor-1α (HIF-1α) , vascular endothelial growth factor A (VEGFA) and vascular endothelial cadherin (VE-cadherin) in cultured rat renal glomerular endothelial cells (rRGECs) exposed to glucose at different concentrations in vitro, and to verify the hypothesis that salidroside attenuates high glucose (HG) -induced injury of rRGECs by boosting HIF-1αlevel.METHODS:rRGECs were divided into 4group:normal glucose (NG) group, HG groups, hypertonic group and salidroside+HG group.The viability of rRGECs was measured by MTT assay.The mRNA expression of VEGFA, VE-cadherin and HIF-1αwas detected by RT-qPCR.The protein expression of HIF-1αwas determined by Western blot.RESULTS:Compared with NG group, the mRNA and protein expression of HIF-1αwas increased when the rRGECs were treated with glucose at concentration of 20 mmol/L for 24h (P<0.01).Compared with NG group, the mRNA expression of HIF-1αwas decreased in HG groups for 120 h (P<0.05).Compared with NG group, the mRNA expression of VE-cadherin was significantly down-regulated in HG groups for24 h or 120 h (P<0.05).Compared with NG group, the mRNA expression of VEGFA was increased in HG groups at 24h (P<0.05) , while the mRNA expression of VEGFA was decreased at 120 h (P<0.05).Compared with NG group, no statistical difference in the mRNA expression levels of HIF-1α, VE-cadherin and VEGFA in DM group was observed.Compared with HG group, salidroside promoted the viability of rRGECs (P<0.01) , and up-regulated the mRNA expression of HIF-1αand VE-cadherin, and the protein expression of HIF-1α (P<0.05).CONCLUSION:High glucose regulates HIF-1αexpression in rRGECs in connection with cell viability, the concentration of glucose, the culture time and HIF/VEGF signaling.Salidroside promotes rRGEC growth against high glucose-induced cell apoptosis via up-regulating the expression of HIF-1α.
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OBJECTIVE: To evaluate the effect of Kuntai capsule on the gonadotrophin releasing hormone agonist(GnRH-a)-induced perimenopaus symptoms and the sex hormone levels.METHODS: A total of 99 patients with uterine fibroids,adenomyosis or moderate to severe endometriosis who needed the treatment of GnRH-a at Sun Yat-sen Memorial Hospital of Sun Yat-sen University from June 2015 to March 2016 were collected and randomly divided into research group(Kuntai capsule)and control group(Tibolone). GnRH-a was injected once every 28 days and first injection of GnRH-a was administered on the 2 nd to 4 th day of menstrual period or retraction bleeding after surgery.Kuntai capsule or Tibolone was orally taken beginning from the first GnRH-a injection,and the co-administration of Caltrate D-600 and alfacalcidol was given in both groups.The Kupperman scores,sex hormone levels including folliclestimulating hormone(FSH)and estrogen(E_2),and adverse events were recorded.RESULTS: Kuntai capsule kept the perimenopause symptoms at mild level with the slow increase of Kupperman scores,whose effect was significantly superior to Tibolone(P<0.05)after 8 weeks of treatment,especially in paresthesia,nervousness,and formication.The FSH and E_2 levels in both Kuntai and Tibolone groups were obviously decreased when compared with the pre-treatment(P<0.05),and these hormone levels in Kuntai group were comparable to those in Tibolone group.No adverse events occurred in either group. CONCLUSION: In the short-term treatment of GnRH-a,Kuntai capsule exhibits significant alleviating effects on perimenopause symptoms caused by GnRH-a with high safety and few adverse reactions.
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<p><b>OBJECTIVE</b>To explore the effect of bone marrow mesenchymal stem cells (MSC) in patients with multiple myeloma(MM) on chemotactic migration of myeloma cells in vitro.</p><p><b>METHODS</b>By in vitro co-culture with diffferent MSC, the myeloma cell U266 was divided into 2 groups: group A in which the U266 cells were co-cultured with normal person MSC (N-MSC) and group B in which the U266 cells were co-cultured with MM-MSC. The expression level of CCR1 in U266 cells, migration rate of U266 cells in Transwell, and the effect of supernantant from co-culture of U266 cells with N-MSC and MM-MSC on the migration in Transwell were compared in condition with or without bortezomib.</p><p><b>RESULTS</b>After co-culture of U266 cells with N-MSC or MM-MSC, the migration rate of U266 cells in Transwell in B group was higher than that in A group(P<0.05). The difference between 2 groups could not be eliminated after treatment of U266 cells with bortezomib. The CCR1 expression level of U266 cells in B group was higher than that in A group (P<0.05). The culture supernatant of bone marrow MSC showed that in condition without bortezomib the culture supernatant of MSC in MM patients and normal persons both possessed more strong chemotactic ability and enhanced the migration rate of cells in Transwell, compared with SDF-1, meanswhile the culture supernatant in 3 groups reduced the migration rate of cells in condition with bortezomib (P<0.05), but there were no statistical difference in migration rate of U266 cells in Transwell between supernatant of N-MSC and MM-MSC culture (P>0.05), no matter the bortezonib was used or not.</p><p><b>CONCLUSION</b>The bone marrow MSC in MM patients have same intrinsic defects that affect the chemotaxis of cells in vitro by directly interacting with myeloma cells.</p>
Subject(s)
Bone Marrow Cells , Bortezomib , Coculture Techniques , Humans , Mesenchymal Stem Cells , Multiple MyelomaABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of exosomes derived from miR-486 gene-modified umbilical cord mesenchymal stem cells (UC-MSCs) on biological characteristics of rat cardiomyocytes.</p><p><b>METHODS</b>The human umbilical cord mesenchymal stem cells (UC-MSCs) were isolated and cultured, then the immunophenotypes and ability of osteogenic and adipogenic differentiation of UC-MSC were identified. The structure of exosomes was observed by electron microscopy; the effect of exosomes on cell migration was detected by transwell cell migration test; the miR-486 high expression of UC-MSC was mediated by using recombinant adenovirus vector, moreover the UC-MSC with high expression of miR-486 were identified by qPT-PCR. The exosomes were isolated from cell culture supernatant by ultracentrifugation and the miR-486 expression level of UC MSC exosomes was detected by qRT-PCR. The effect of exosomes on the proliferation of cardiomyocytes was evaluated by Dye670 marking. The HO-induced cardiomyocyte apoptosis model was established, and the effect of exosomes on apoptosis of cardiomyocytes was detected by flow cytometry with Annexin V/PI double staining.</p><p><b>RESULTS</b>The exosomes derived from UC-MSCs had the diameter between 40-100 nm and double membrane stracture. The recombinant adenovirus could effectively mediate the expression of miR-486 in UC-MSC, and the expression level of miR-486 in exosomes of miR-486-modified UC-MSC significantly increased. The exosomes with miR-486 high expression possessed the pro-proliferation and pro-migration effects on cardiomyocytes, moreover the preventive effect on apoptosis of cardiomyocytes.</p><p><b>CONCLUSION</b>The exosomes derived from UC-MSC and accompamied by high expression of miR-486 can promote the proliferation and migration of cardio myocytes, yet can prevent the apoptosis of cardiomyocytes.</p>
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Objective To investigate the effect of the family nursing intervention on treatment of children with asthma, and explore the effect of family nursing intervention on the pulmonary function and quality of life in asthmatic children. Methods A total of 260 children with asthma were recruited at the First Affiliated Hospital of Xi'an Jiaotong University from July 2012 to July 2015. They were randomly divided into observation and control groups with 130 cases in each group. The control group were given routine nursing and regular treatment, and the observation group were received additional family nursing intervention management at the same time. We use Pediatric Asthma Quality of Life Questionnaire (PAQLQ) and pulmonary function testing to evaluate the influence of family nursing intervention on treatment effect and quality of life of asthmatic children. Results The total effective rate was 93% in the observation group and was 65% in the control group (P＜0.05) . The pulmonary function and quality of life were significant higher in the intervention group compared with the control group after intervention. Parents’satisfaction was significantly higher in the intervention group (P＜0.05) . Conclusion Family nursing intervention is an effective mode of management of children with asthma that can obviously improve pulmonary function and quality of life of asthmatic children. Moreover, family nursing intervention also contributes to raising parents'satisfaction.