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1.
Article in Chinese | WPRIM | ID: wpr-905309

ABSTRACT

Objective:To explore the effect of cognitive-motor dual task training on vascular mild cognitive impairment (VaMCI) in old patients. Methods:From March to September, 2019, 76 old patients with VaMCI in three general hospitals were divided into control group (n = 38) and intervention group (n = 38). All the patients accepted health education, while the intervention group accepted cognitive-motor dual task training in addition, for three months. They were assessed with Montreal Cognitive Assessment-Beijing Version (MoCA-BJ) and Trail Making Test (TMT-A) before and after intervention, and the one-year incidence of fall before and after intervention was investigated in the intervention group. Results:Two cases in the control group and three cases in the intervention group dropped down. The total score and dimension scores of MoCA-BJ were more significantly in the intervention group than in the control group after intervention (|Z| > 2.002, P < 0.05), except the dimension of naming, while the time for TMT-A was significantly less (Z = -5.949, P < 0.001). The one-year incidence of fall decreased significantly during the year after intervention (χ2 = 10.080, P < 0.01). Conclusion:Cognitive-motor dual task training can improve the cognitive function of older VaMCI patients, especially for attention and executive function, and reduce the risk of fall.

2.
Article in Chinese | WPRIM | ID: wpr-879899

ABSTRACT

OBJECTIVE@#To evaluate the clinical features of preterm infants with a birth weight less than 1 500 g undergoing different intensities of resuscitation.@*METHODS@#A retrospective analysis was performed for the preterm infants with a birth weight less than 1 500 g and a gestational age less than 32 weeks who were treated in the neonatal intensive care unit of 20 hospitals in Jiangsu, China from January 2018 to December 2019. According to the intensity of resuscitation in the delivery room, the infants were divided into three groups:non-tracheal intubation (@*RESULTS@#Compared with the non-tracheal intubation group, the tracheal intubation and ECPR groups had significantly lower rates of cesarean section and use of antenatal corticosteroid (@*CONCLUSIONS@#For preterm infants with a birth weight less than 1 500 g, the higher intensity of resuscitation in the delivery room is related to lower rate of antenatal corticosteroid therapy, lower gestational age, and lower birth weight. The infants undergoing tracheal intubation or ECRP in the delivery room have an increased incidence rate of adverse clinical outcomes. This suggests that it is important to improve the quality of perinatal management and delivery room resuscitation to improve the prognosis of the infants.


Subject(s)
Birth Weight , Cesarean Section , China , Female , Gestational Age , Humans , Infant , Infant, Newborn , Infant, Premature , Pregnancy , Retrospective Studies
3.
Article in Chinese | WPRIM | ID: wpr-905461

ABSTRACT

Objective:To investigate the relationship between gait parameters under dual-task condition and falls in old patients with vascular mild cognitive impairment (VaMCI). Methods:From March to September, 2019, convenience sampling method was adopted to select 103 old patients with VaMCI. They were divided into falling group (n = 45) and non-falling group (n = 58), according to the situation of falling in the past one year. Both groups were surveyed general data, assessed with modified Barthel Index (MBI) and Timed “Up and Go” Test (TUGT), and collected gait parameters under dual-task condition. Results:MBI score was significantly higher (t = 35.688, P < 0.001), and the time of TUGT was shorter (t = 2.428, P < 0.05) in the non-falling group than in the falling group. The speed, length, frequency, swing phase and ground impact were higher (t > 2.536, |Z| > 1.986, P < 0.05), and single step time and the stance phase were lower (|Z| > 2.440, P < 0.05) in the non-falling group than in the falling group. Speed, length, frequency and single step time were risk factors for falling (P < 0.05). Conclusion:The characteristics of gait parameters under dual-task condition could reflect the walking ability of the old patients with VaMCI, suggesting the risk of fall, especially speed, length, frequency and single step time, which need to be measured and intervened in time.

4.
Acta Physiologica Sinica ; (6): 537-546, 2019.
Article in Chinese | WPRIM | ID: wpr-777158

ABSTRACT

Intermittent hypoxia (IH) has preventive and therapeutic effects on hypertension, myocardial infarction, cerebral ischemia and depression, but its effect on post-traumatic stress disorder (PTSD) has not been known. In this study, we used inescapable electric foot shock combined with context recapture to build PTSD mouse model. The levels of fear and anxiety were valued by the open field, the elevated plus maze (EPM) and the fear conditioning tests; the level of spatial memory was valued by Y maze test; the number of Fos positive neurons in hippocampus, amygdala and medial prefrontal cortex was valued by immunohistochemical staining; and the protein expressions of hypoxia inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and brain derived neurotrophic factor (BDNF) in these brain area were valued by Western blot. The results showed that IH and model (foot shock) had an interaction on percentage of entering open arms (OE%) in EPM and freezing time and the number of fecal pellets in fear conditioning test. IH increased OE% in EPM and reduced the freezing time and the number of fecal pellets in fear conditioning test in PTSD model mice. At the same time, IH reduced the number of Fos positive neurons in the hippocampus, amygdala and medial prefrontal cortex of PTSD model mice, and increased the protein expression levels of HIF-1α, VEGF and BDNF in these brain tissues. In conclusion, IH pretreatment can relieve fear and anxiety behavior in post-traumatic stress model mice, suggesting that IH may be an effective means of preventing PTSD.


Subject(s)
Animals , Anxiety , Therapeutics , Brain-Derived Neurotrophic Factor , Metabolism , Fear , Hypoxia , Hypoxia-Inducible Factor 1, alpha Subunit , Metabolism , Mice , Stress Disorders, Post-Traumatic , Therapeutics , Vascular Endothelial Growth Factor A , Metabolism
5.
Article in Chinese | WPRIM | ID: wpr-776538

ABSTRACT

OBJECTIVE@#To investigate the effects of simulated hypobaric hypoxia environment at 7 000 m above sea level on cardiac structure and function in rats.@*METHODS@#A total of 96 male SD rats were randomly divided into high-altitude hypobaric hypoxia group (hypoxia group) and normobaric normoxia group (control group). Rats of hypoxia group were placed in a large cabin simulated 7 000 m high-altitude hypobaric hypoxia environment. Operating time 23 h / d, the control circadian ratio of approximately 12 h:12 h. The rats in control group were bred under normobaric normoxia. The hypoxic group was divided into 3 d, 7 d, 14 d, 28 d groups according to hypoxic time, 12 rats in each group. Changes of structure and function of heart due to hypoxia were evaluated by echocardiography and electrocardiogram. Myocardial pathological changes were analyzed by hematoxylin-eosin staining(HE).@*RESULTS@#Compared with the control group at the same time point ①With prolonged exposure to hypobaric hypoxia, the growth ratio of body mass in rats is slower. Arterial oxygen saturation was significantly lower in both 14 d and 28 d (P<0.05). ② Left ventricular end-diastolic anterior wall thickness (LVAWD) and left ventricular end-diastolic posterior wall thickness (LVPWD) of rats in 28 d were increased significantly (P<0.05). Left ventricular end-diastolic diameter (LVIDD) and left ventricular internal dimension systole (LVIDS) of rats in 28 d were decreased significantly (P<0.05, P<0.01). Left ventricular ejection fraction (EF), fractional shortening of left ventricle (FS), pulmonary vein (PV) peak velocity and PV peak gradient of rats in 7 d were decreased significantly (P<0.05, P<0.01). ③The QRS and QT interval period were significantly prolonged in 14 d and 28 d (P<0.05, P<0.01). The ST was significantly lower in 3 d and 7 d (P<0.05, P<0.01). The amplitude of R wave gradually shifted downward in 7 d, 14 d, 28 d (P<0.05, P<0.01). ④The red blood cell (RBC), hemoglobin (HGB), red blood cell distribution width (RDW) in hypoxic group were increased significantly (P<0.01). The platelet count (PLT) count was decreased significantly in 14 d and 28 d (P<0.01). The serum creatinine (CR) was increased significantly in 14 d and 28 d (P<0.05). ⑤Pathological changes such as myocardial edema, sarcolemma condensate, focal degeneration and necrosis with inflammatory cell infiltration could be found at early stage of hypoxia. Myocardial compensatory repair such as myocardial fibroblasts proliferation was significant at end stage of hypoxia.@*CONCLUSION@#Left ventricular systolic functions of rats were decreased significantly after exposure to high altitude hypoxia hypobaric. The left ventricular systolic functions would recovery compensatory after one week exposed to high altitude hypoxia hypobaric.


Subject(s)
Altitude , Animals , Heart , Hypoxia , Male , Rats , Rats, Sprague-Dawley
6.
Article in Chinese | WPRIM | ID: wpr-776521

ABSTRACT

OBJECTIVE@#To detect the effects of metformin on the depressive-like behaviors in rats.@*METHODS@#Forty male SD rats were randomly divided into four groups: control group (CON group), metformin group (MET group), model group (CUMS group), model + metformin group (CUMS + MET group), 10 rats in each group. Chronic unpredictable mild stress (CUMS) method was used to establish rat depression model in three weeks. After the model was established successfully, two metformin groups were intraperitoneally injected with metformin (100 mg/kg), while the control group and the model group were injected with the same amount of saline once a day for two weeks. After that, the changes of weight gain, sucrose water preference experiment, forced swimming test, tail suspension immobility test and open field test were detected. The morphological changes of hippocampus were observed by Nissl staining.@*RESULTS@#Compared with the control group, the weight gain of rats in CUMS group was significantly slowed down (P<0.05), the sucrose preference rate and the spontaneous activity were significantly reduced (P<0.05), and the immobility time in forced swimming and tail suspension immobility test was significantly prolonged (P<0.05), and the morphological structure of hippocampus was changed, which confirmed the success of CUMS depression model. Compared with CUMS group, metformin treatment had no significant effect on body weight of rats, but it could significantly improve sucrose water intake, immobility time and spontaneous activity of CUMS depression model rats (P<0.05), and improve the abnormal morphological changes of hippocampus in CUMS rats.@*CONCLUSION@#Metformin has a therapeutic benefit against CUMS-induced depression, which provides a new treatment for patients with diabetes mellitus complicated with depression.


Subject(s)
Animals , Depression , Drug Therapy , Hippocampus , Male , Metformin , Pharmacology , Random Allocation , Rats , Rats, Sprague-Dawley , Stress, Psychological
7.
Article in Chinese | WPRIM | ID: wpr-773813

ABSTRACT

OBJECTIVE@#To investigate the effects of deficiency of CHL1 in inflammatory bowel disease (IBD).@*METHODS@#Dextran Sulfate Sodium (DSS)-induced colitis model was used to study the effects of deficiency of CHL1 on the development of IBD. Ten CHL1(+/+) mice in C57/BL6 background were randomly divided into CHL1(+/+) group and DSS-induced CHL1(+/+) group. Ten CHL1(-/-) mice in C57/BL6 background were randomly divided into CHL1(-/-) group and DSS-induced CHL1(-/-) group. DSS-induced CHL1(+/+) group and DSS-induced CHL1(-/-)group were fed with 1.5% DSS for 7 days, and then drinking distilled water for 2 days. CHL1(+/+) group and CHL1(-/-) group as control group were fed with distilled water for 9 days. The changes of weight, survival, fecal blood and the change of colon length in this study were observed.@*RESULTS@#On the 7 day, the weight of DSS-induced CHL1(-/-) group were reduced significantly, and DSS-induced CHL1(-/-) group had extreme mortality on the 9th day. The fecal blood of DSS-induced CHL1(-/-) group also had higher score than that of DSS-induced CHL1(+/+) group. In the DSS-induced CHL1(-/-) group,the length of colon was shortened obviously.@*CONCLUSIONS@#The loss of CHL1 aggravates the development of IBD.


Subject(s)
Animals , Cell Adhesion Molecules , Genetics , Colitis , Genetics , Colon , Pathology , Dextran Sulfate , Disease Models, Animal , Mice , Mice, Inbred C57BL , Random Allocation
8.
Neuroscience Bulletin ; (6): 1058-1066, 2018.
Article in English | WPRIM | ID: wpr-775472

ABSTRACT

While inflammatory bowel disease (IBD) might be a risk factor in the development of brain dysfunctions, the underlying mechanisms are largely unknown. Here, mice were treated with 5% dextran sodium sulfate (DSS) in drinking water and sacrificed on day 7. The serum level of IL-6 increased, accompanied by elevation of the IL-6 and TNF-α levels in cortical tissue. However, the endotoxin concentration in plasma and brain of mice with DSS-induced colitis showed a rising trend, but with no significant difference. We also found significant activation of microglial cells and reduction in occludin and claudin-5 expression in the brain tissue after DSS-induced colitis. These results suggested that DSS-induced colitis increases systemic inflammation which then results in cortical inflammation via up-regulation of serum cytokines. Here, we provide new information on the impact of colitis on the outcomes of cortical inflammation.


Subject(s)
Animals , Calcium-Binding Proteins , Metabolism , Caspase 3 , Metabolism , Cerebral Cortex , Pathology , Claudin-5 , Metabolism , Colitis , Pathology , Cytokines , Genetics , Metabolism , Dextran Sulfate , Toxicity , Disease Models, Animal , Encephalitis , Gene Expression Regulation , Mice , Microfilament Proteins , Metabolism , Occludin , Metabolism , Polysaccharides , Blood , Toxicity , Time Factors
9.
Article in Chinese | WPRIM | ID: wpr-705229

ABSTRACT

The size of the plateau in China is large and the plateau environment concerns our economic construction and national security because of its special location. The average altitude of the Qinghai-Tibet plateau,which is of great military and economic significance,is above 4000 m.The special plateau environment,such as hypoxia and low temperature,poses a serious threat to the physical and mental health of the populations there.In recent years,with the development of neuroscience and technology, the impact of plateau environment hypoxia on human cognitive function has received more attention. Here we review the impact of plateau environment on the human cognitive function and related interven-tion measures to provide reference,for protection of cognitive ability and mental ability at high altitude.

10.
Chinese Journal of Nursing ; (12): 1495-1499, 2017.
Article in Chinese | WPRIM | ID: wpr-665004

ABSTRACT

Objective To establish a nursing performance evaluation indicator system in military hospitals,so as to provide reference for standardized nursing performance management. Methods Based on literature review,group discussion and qualitative interviews,two rounds of consultation were conducted among 19 experts using Delphi method. Results The nursing performance evaluation indicator system in military hospitals included 3 first-level in-dicators,13 second-level indicators and 60 third-level indicators. Experts' authority coefficient,judgment coefficient and familiarity coefficient were 0.930,0.960,0.900,respectively. The coordination coefficients of the first-level,sec-ond-level and third-level were 0.517,0.490 and 0.459. Variation coefficients were 0~0.113. Conclusion The nurs-ing performance evaluation indicator system in military hospitals based on Delphi method is scientific and reliable, which provides references for nursing performance in clinical units and makes significant contributions to nursing performance management in military hospitals.

11.
Military Medical Sciences ; (12): 652-657, 2017.
Article in Chinese | WPRIM | ID: wpr-664428

ABSTRACT

Objective To investigate the effect of metformin on rat adrenal pheochromocytoma PC12 cell injury induced by hypoxia and its possible molecular mechanisms.Methods PC12 Cells were cultured under hypoxia conditions (0.3% O2).The cell survival and apoptosis were detected by methylrosanilinium chloride (crystal violet) staining and Annexin-V/propidium iodide (PI) staining,the cell viability measured by CCK8 assay,and the signaling activity and protein levels were detected by Western blotting.Results Physical hypoxia at an O2 concentration of 0.3% resulted in time-dependent injury to PC12 cells,while metformin pretreatment had protective effect on PC12 cell injury induced by hypoxia.AMP-activated protein kinase (AMPK) signaling was activated by metformin treatment,and AMPK kinase inhibitor compound C blocked the protective effect of metformin on hypoxia-induced PC12 cell injury.Conclusion These results demonstrate that metformin pretreatment has protective effect on PC12 cell injury induced by hypoxia,and AMPK signaling plays an important role in the protective effect of metformin.

12.
Acta Physiologica Sinica ; (6): 737-742, 2017.
Article in Chinese | WPRIM | ID: wpr-348224

ABSTRACT

High-intensity sound often leads to the dysfunction and impairment of central nervous system (CNS), but the underlying mechanism is unclear. The present study was aimed to investigate the related mechanisms of CNS lesions in Bama miniature pig model treated with high-intensity sound. The pigs with normal hearing were divided into control and high-intensity sound (900 Hz-142 dB SPL, 15 min) groups. After the treatment, hippocampi were collected immediately. Fluo-4 was used to indicate intracellular Caconcentration ([Ca]) change. Real-time PCR and Western blot were used to detect mRNA and protein expressions of calcium-sensing receptor, L-Cachannel α2/δ1 subunit, PKC and PI3K, respectively. DAPI staining was used to identify nuclear features. The result showed that high-intensity sound exposure resulted in significantly swollen cell nucleus and increased [Ca]in hippocampal cells. Compared with control group, high-intensity sound group showed increased levels of PI3K, PKC and L-Cachannel α2/δ1 subunit mRNA expressions, as well as up-regulated PKC and calcium-sensing receptor protein expressions. These results suggest that the high-intensity sound activates PKC signaling pathway and induces calcium overload, eventually leads to hippocampal injury, which would supply a novel strategy to prevent nervous system from high-intensity sound-induced injury.

13.
Article in Chinese | WPRIM | ID: wpr-256563

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effect of rhynchophylline on methamphetamine-dependent zebrafish and explore the possible mechanism.</p><p><b>METHODS</b>Zebrafish were divided into control group, amphetamine group, low- (50 mg/kg) and high (100 mg/kg)-dose rhynchophylline groups, and ketamine (150 mg/kg) group. Conditioned place preference (CPP) was induced in zebrafish with methamphetamine, and the staying time in the drug box and the tracking map of the zebrafish were observed with Noldus Ethovision XT system. The protein expressions of TH, NR2B and GLUR2 in the brain of zebrafish with CPP were detected with Western blotting.</p><p><b>RESULTS</b>Compared with the control group, zebrafish in methamphetamine group showed significant variations in the staying time and swimming distance in the drug box after conditioning (P<0.05) with obvious alterations of NR2B, TH and GLUR2 expressions in the brain (P<0.05). Treatment of methamphetamine-dependent zebrafish with high-dose rhynchophylline significantly reduced the variations in the staying time and swimming distance in the drug box (P<0.05) and in the expressions of NR2B, TH and GLUR2 in the brain (P<0.05).</p><p><b>CONCLUSION</b>Rhynchophylline can inhibit methamphetamine dependence in zebrafish, the mechanism of which may involve the expressions of TH, NR2B and GLUR2 proteins in the brain.</p>

14.
Acta Physiologica Sinica ; (6): 126-134, 2016.
Article in Chinese | WPRIM | ID: wpr-331675

ABSTRACT

The aim of this study was to develop a murine model of brain injury induced by high altitude hypoxic inflammation. In the study, we used a decompression chamber to mimic an acute hypobaric hypoxia, and 8-week-old male C57BL/6 mice were intraperitoneally injected with 5 mg/kg lipopolysaccharide (LPS) to induce inflammatory response. We determined the levels of pro-inflammatory factors (IL-6, TNF-α) and anti-inflammatory factor (IL-10) in mice serum using ELISA assays to confirm the high altitude hypoxic inflammation, and verified the brain injury after the inflammation using hematoxylin-eosin (HE) staining. The results showed that, among four experiment groups (ctrl, acute hypobaric hypoxia, LPS, and acute hypobaric hypoxia plus LPS groups), the acute hypobaric hypoxia plus LPS treatment group displayed the highest levels of IL-6, TNF-α, and IL-10. Meanwhile, the acute hypobaric hypoxia plus LPS treatment group showed the most severe cortex and hippocampus injuries, including cellular swelling, the widened pericellular spaces, angiogenesis, and shrunken neurons with darkly stained pyknotic nuclei, etc. Strikingly, nuclei ventrales posteriors thalami were found to be more sensitive to acute hypobaric hypoxia plus LPS treatment, and their destroy degrees were higher than those neurons in cortex and hippocampus. These results suggested that we established a reliable murine model of brain injury induced by high altitude hypoxic inflammation, and might be useful to the relevant studies.


Subject(s)
Altitude , Animals , Brain Injuries , Cerebral Cortex , Disease Models, Animal , Hippocampus , Hypoxia , Inflammation , Male , Mice , Mice, Inbred C57BL , Neurons
15.
Article in Chinese | WPRIM | ID: wpr-236373

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effects of high altitude on cognitive flexibility.</p><p><b>METHODS</b>Simulated hypoxia at an altitude of 3 600 m was performed in a hypobaric chamber. Twenty-three volunteers without hypoxic experience were selected and the mean age was about 25.1 years. The physiological parameters (heart rate, blood pressure and oxygen saturation) were measured. Task switch paradigm was used to explore the cognitive flexibility in each phase, and the changing anxiety state was evaluated simultaneously.</p><p><b>RESULTS</b>Reaction time (RT) switch cost in hypoxia phase showed a significant increase compared with the baseline; anxiety level in hypoxia phase was higher than the adaptation phase; a remarkable negative correlation between anxiety level and RT switch cost was found in adaptation phase, whereas a positive correlation was found in landing phase.</p><p><b>CONCLUSION</b>High altitude (3 600 m) affects cognitive flexibility and anxiety state. Anxiety before the hypoxia exposure improves the cognitive flexibility performance, while anxiety after the hypoxia exposure hampers the performance because of the post-hypoxia effect.</p>


Subject(s)
Adult , Altitude , Anxiety , Cognition , Physiology , Humans , Hypoxia , Psychology , Male , Reaction Time
16.
Article in Chinese | WPRIM | ID: wpr-235404

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the regulation of different hypoxia on cell survival and autophagy.</p><p><b>METHODS</b>PC12 cells were treated with different hypoxia. The cell survival was measured by MTT assay, expressions of LC3 and p62 were marked for autophagy detected by Western Blot, and the level of reactive oxygen species (ROS) was analyzed by flow cytometry.</p><p><b>RESULTS</b>The cell viability was different under different hypoxia: moderate hypoxia promoted cell viability, and severe hypoxia caused a decrease in cell viability; autophagy marker molecules, p62 and LC3-II expressions were different: moderate hypoxia increased p62 and LC3-II expressions, in contrast, severe hypoxia led to the decrease of p62 and LC3-II expressions; compared to normoxia, moderate hypoxia did not change the levels of ROS, while severe hypoxia increased the levels; 3-MA, the inhibitor of autophagy, elevated the levels of ROS in the three oxygen concentrations, additionally, the increased amplitudes in the moderate and severe hypoxia groups were higher than that in the normoxia group.</p><p><b>CONCLUSION</b>Moderate hypoxia promotes cell survival, severe hypoxia causes the cell death, and the autophagy activity may mediate the effects of different hypoxia.</p>


Subject(s)
Animals , Autophagy , Physiology , Cell Death , Cell Hypoxia , Cell Survival , PC12 Cells , Rats , Reactive Oxygen Species , Metabolism
17.
Article in Chinese | WPRIM | ID: wpr-235394

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the change of gastric cancer cell proliferation and the expression of gastric cancer related gene 213 (GCRG213), a long interspersed nuclear element-1 (LINE-1) endonuclease variant, during hypoxia.</p><p><b>METHODS</b>Normal gastric mucosa cell GES-1 and gastric cancer cell BGC-823 were cultured in 20% or 3% oxygen concentrations, respectively. MTT test was used to analyze the proliferation of the GES-1 and BGC-823 cells. The change of GCRG213 mRNA and protein expression in GES-1 and BGC-823 cells was detected by using RT-PCR and Western blot analysis. Blast was used at the NCBI Blast server to identify GCRG213 sequence to any alignment in the GeneBank databases.</p><p><b>RESULTS</b>Compared with 20% oxygen condition, 3% oxygen concentration could promote cell growth. Mean-while, the expression of GCRG213 at mRNA and protein levels was increased. GCRG213 sequence shared high homology with LINE-1 endonuclease sequence.</p><p><b>CONCLUSION</b>GCRG213 is a variant of LINE-1 endonuclease. Hypoxia as in 3% oxygen condition can promote cell proliferation and lead to GCRG213 overexpression.</p>


Subject(s)
Cell Line, Tumor , Cell Proliferation , Cells, Cultured , Deoxyribonuclease I , Genetics , Gastric Mucosa , Cell Biology , Gene Expression , Humans , Hypoxia , Peptide Hormones , Genetics , Stomach Neoplasms , Genetics , Pathology
18.
Article in Chinese | WPRIM | ID: wpr-247026

ABSTRACT

<p><b>OBJECTIVE</b>To assess the clinical efficacy of acupuncture pretreatment for the prevention of stroke based on promoting the circulation of the Governor Vessel and regulating mentality, and explore its effect mechanism.</p><p><b>METHODS</b>Seventy cases of transient ischemic attack (TIA) were randomized into an acupuncture group (35 cases) and a western medicine group (35 cases). In the acupuncture group, acupuncture therapy of promoting the circulation of the Governor Vessel and regulating mentality was applied at Yaoyangguan (GV 3), Mingmen (GV 4), Zhiyang (GV 9), Shenzhu (GV 12), Dazhui (GV 14), Yamen (GV 15), Fengfu (GV 16), Baihui (GV 20) and Neck-Jiaji (EX-B 2). Acupuncture was given 6 times a week, at the interval of one day between two weeks. Totally, 21 days of treatment were taken as a session. In the western medicine group, aspirin enteric coated tablets were prescribed, 25 mg/tablet, 100 mg/day, once a night for oral administration, and 21 days of medication were taken as 1 session. There were 3 days at the interval between two sessions in each group and totally 2 sessions were required. Transcranial Doppler (TCD) was adopted before treatment and in two sessions of treatment to observe, mean flow velocity (Vm) of middle cerebral artery (MCA), vertebral artery (VA), basilar arte ry (BA) and pulsatility index (PI). The standard of the efficacy assessment of stroke aura was taken as the main efficacy index in the assessment of theraputic effect, the adverse reaction was observed.</p><p><b>RESULTS</b>1In TIA, MCA blood flow was accelerated in internal carotid system, and BA blood flow was accelerated in vertebral-basilar artery system. The treatments in the two groups enabled the blood flow in the responsible blood vessels slow down and the results in the acupuncture group L(60. 54+/-11.76)cm/s, (36. 17+/-8. 65)cm/s] were better than those in the western medicine group [(72. 34+/-9. 15)cm/s,(65. 23 +/-8. 99)cm/s] (P<O. 05). 2The results of clinical efficacy and adverse reactions in the acupuncture group [96. 77% (30/31),6. 45% (2/31)] were superior to the western medicine group [75. 76%(25/33),45. 46%(15/33)] (both P<0. 05). 3Concerning to the recurrence of disease at the different period after treatment, the case number of cerebral infarction was not different significantly between the two groups (P>0. 05).</p><p><b>CONCLUSION</b>Acupuncture intervention based on promoting the circulation of the Governor Vessel and regulating mentality achieves the superior efficacy on TIA and less adverse reactions as compared with aspirin. The effect mechanism is related potentially to the improvement of cerebral vascular hemodynamic.</p>


Subject(s)
Acupuncture Therapy , Adult , Aged , Female , Humans , Male , Middle Aged , Stroke , Therapeutics , Treatment Outcome
19.
Article in Chinese | WPRIM | ID: wpr-308597

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of houttuyfonate sodium (HS) on eliminating adhesion of Psedomonas aeruginosa (Pa) and forming biofilms.</p><p><b>METHOD</b>Pa biofilms were established in 96-hold plates. MTT assay was used to evaluate the changes in metabolism of biofilms and assess the minimum eliminating concentration and minimum biofilm inhibitory concentration for adherent Pa. The colony counting method was used to observe the effect of HS on Pa adhesion and biomass in biofilms. SEM was employed to examine the effect of HS on adhesion of tested Pa and morphology of biofilms.</p><p><b>RESULT</b>MEC80 and MEC50 of HS for adherent Pa was 500 mg x L(-1) and 125 mg x L(-1), respectively. Meanwhile, its SMIC80 for either early or mature biofilms of Pa was 500 mg x L(-1), and SMIC50 for early and mature biofilms of Pa were 31.25, 1.95 mg x L(-1), respectively. At the concentration of 250 mg x L(-1), the number of viable bacteria in the state of adhesion and in initial and mature biofilms decreased significantly, compared with the control group (P < 0.05). The number of bacteria on adherent carriers notably reduced under SEM. Following the continuous administration, there were no visible biofilms on carriers in the mature biofilm phase, with the biomass remarkably shrinking and the bacterial morphology changing from bacillus into coccobacillus.</p><p><b>CONCLUSION</b>HS displayed powerful effect on eliminating adherent Pa, and can inhibit Pa biofilm from being formed through continuous administration.</p>


Subject(s)
Alkanes , Pharmacology , Anti-Bacterial Agents , Pharmacology , Bacterial Adhesion , Biofilms , Microbial Sensitivity Tests , Pseudomonas aeruginosa , Sulfites , Pharmacology
20.
Article in Chinese | WPRIM | ID: wpr-351176

ABSTRACT

<p><b>OBJECTIVE</b>To observe the effects of acute hypoxia on the cell adhesion molecule close homologue of L1 (CHL1) expression in different brain areas and main organs (heart, lung, kidney) of mice, and provide a basis for the role of CHL1 in hypoxia injury.</p><p><b>METHODS</b>Mice were randomly divided into two groups (n=10): normoxia group and hypoxia group. Hypoxia group were treated by acute hypoxia (8% O2, 8 h). Protein expression changes in different tissues were evaluated by Western blot.</p><p><b>RESULTS</b>In central nervous system, CHL1 protein expressions were down-regulated in cerebral cortex, hypothalamus and brain stem by acute hypoxia and up-regulated in cerebellum. In heart and lung, CHL1 protein expression were down-regulated by acute hypoxia.</p><p><b>CONCLUSION</b>CHL1 protein expressions were changed in different tissues after acute hypoxia, which suggested CHL1 might play an important role in hypoxia damage regulation.</p>


Subject(s)
Animals , Brain , Metabolism , Cell Adhesion Molecules , Genetics , Metabolism , Hypoxia , Metabolism , Lung , Metabolism , Male , Mice , Myocardium , Metabolism , Tissue Distribution
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