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1.
Chinese Journal of Dermatology ; (12): 116-122, 2022.
Article in Chinese | WPRIM | ID: wpr-933521

ABSTRACT

Objective:To determine the expression of silent information regulator 1 (Sirt1) , Sirt3 and hypoxia-inducible factor 1α (HIF-1α) in cutaneous squamous cell carcinoma (CSCC) tissues and cells, and to explore their role in the occurrence and development of CSCC.Methods:From January 2019 to December 2020, 30 lesional skin tissues were obtained from patients with histopathologically confirmed poorly-, moderately- or well-differentiated CSCC, and 30 normal skin tissues were obtained from patients with non-cancerous diseases in Department of Dermatology, General Hospital of Ningxia Medical University. A CSCC cell line A431 and a human keratinocyte cell line HaCaT were cultured. Immunohistochemical study, Western blot analysis and real-time quantitative PCR (RT-PCR) were performed to determine the protein and mRNA expression of Sirt1, Sirt3 and HIF-1α in CSCC tissues of different grades of differentiation and normal skin tissues, cytochemical and immunofluorescence staining and RT-PCR were conducted to determine the protein and mRNA expression of Sirt1, Sirt3 and HIF-1α in A431 and HaCaT cells, respectively. Comparisons of measurement data among multiple groups were performed by using one-way analysis of variance, and comparisons between two groups by using t test. Results:Immunohistochemical study showed that the expression level of Sirt3 (expressed as the average optical density) was 100 ± 12.12, 117.72 ± 26.23, 127.32 ± 24.45, 132.71 ± 31.61 in the normal skin tissues and well-, moderately- and poorly-differentiated CSCC tissues respectively, and there was a significant difference among these groups ( F = 20.14, P < 0.001) ; the expression of Sirt1 and HIF-1α increased in turn from the normal skin tissues to the well-, moderately- and poorly-differentiated CSCC tissues, and significantly differred in these groups ( F = 174.50, 225.00, respectively, both P < 0.001) . As Western blot analysis revealed, the expression level of Sirt3 significantly differed among the normal skin tissues, well-, moderately- and poorly-differentiated CSCC tissues (expressed as relative gray value: 1.000 ± 0.132, 1.403 ± 0.411, 1.387 ± 0.393, 1.677 ± 0.683, respectively; F = 34.97, P < 0.001) , and so did the expression levels of Sirt1 and HIF-1α ( F = 69.29, 199.90, respectively, both P < 0.00l) , with a gradually increasing trend in their expression levels from the the normal skin tissues to well-, moderately- and poorly-differentiated CSCC tissues. RT-PCR showed that the mRNA expression of Sirt3, Sirt1 and HIF-1α was sequentially increased from the normal skin tissues to well-, moderately- and poorly-differentiated CSCC tissues, and significant differences were observed among these groups ( F = 113.00, 174.50, 50.33, respectively, all P < 0.001) . The protein expression levels of Sirt3, Sirt1 and HIF-1α were significantly higher in the A431 cells than in the HaCaT cells ( t = 16.75, 18.34, 27.76, respectively, all P < 0.001) , and so were their mRNA expression levels ( t= 14.22, 9.62, 16.86, respectively, all P < 0.001) . Conclusion:Increased expression of Sirt3, Sirt1 and HIF-1α was observed in CSCC tissues and cells, which may promote the occurrence and development of CSCC.

2.
Article in Chinese | WPRIM | ID: wpr-911247

ABSTRACT

Objective:To evaluate the role of heme oxygenase-1 (HO-1) in sevoflurane-induced improvement in sepsis-associated encephalopathy (SAE) in mice.Methods:A total of 136 adult male mice, aged 6-8 weeks, weighing 20-25 g, were divided into 4 groups ( n=34 each) using a random number table method: sham operation group (group Sham), SAE group, SAE+ sevoflurane group (group SAE+ Sevo) and SAE+ sevoflurane+ HO-1 inhibitor Zn Protoporphyrin Ⅸ (ZnPPⅨ) group (group SAE+ Sevo+ ZnPPⅨ). The model of SAE was established by cecal ligation and puncture (SAE) in anesthetized mice.In SAE+ Sevo and SAE+ Sevo+ ZnPPⅨ groups, 2% sevoflurane-33% oxygen was inhaled for 2 h starting from the time point immediately after establishment of the model, while 33% oxygen was inhaled for 2 h in Sham and SAE groups.ZnPPⅨ 25 mg/kg was intraperitoneally injected at 30 min before the model was established in group SAE+ Sevo+ ZnPPⅨ.Six mice were sacrificed at 6, 12 and 24 h after establishment of the model for determination of levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), high mobility group protein B1 (HMGB1) and HO-1 in cortical tissues (by enzyme-linked immunosorbent assay) and the expression of HO-1 (by Western blot). Another 6 mice were sacrificed for determination of apoptosis in cortical tissue (by TUNEL staining), and apoptotic index (AI) was calcultated.Ten mice in each group were selected, Y maze test was performed at 3, 5, 7 and 14 days after establishment of the model, and the percentage of spontaneous alternation was calculated. Results:Compared with Sham group, the levels of TNF-α, IL-1β and HMGB1, AI, HO-1 activity and its expression level in cortex were significantly increased, and the percentage of spontaneous alternation was decreased in SAE, SAE+ Sevo and SAE+ Sevo+ ZnPPⅨ groups ( P<0.05). Compared with group SAE, the levels of TNF-α, IL-1β and HMGB1 and AI were significantly decreased, and HO-1 activity and its expression level and the percentage of spontaneous alternation were increased in group SAE+ Sevo, the levels of TNF-α, IL-1β and HMGB1 in cortex were decreased ( P<0.05), and no significant change was found in the other parameters in group SAE+ Sevo+ ZnPPⅨ ( P>0.05). Compared with group SAE+ Sevo, the levels of TNF-α, IL-1β and HMGB1 and AI were significantly increased, and HO-1 activity and its expression level and the percentage of spontaneous alternation were decreased in group SAE+ Sevo+ ZnPPⅨ ( P<0.05). Conclusion:The mechanism by which sevoflurane improves SAE is related to increasing HO-1 activity and reducing inflammatory response in cortical tissues of mice.

3.
Article in Chinese | WPRIM | ID: wpr-909203

ABSTRACT

Objective:To investigate the efficacy and safety of Xiatianwu eye drops in the treatment of pseudomyopia in children. Methods:A total of 8 000 children with pseudomyopia who received treatment in Jiaxing Hospital of Traditional Chinese Medicine between June 2018 and June 2019 were included in this study. They were randomly assigned to receive treatment with either raceanisodamine eye drops (control group, n = 4 000) or Xiatianwu eye drops combined with raceanisodamine eye drops (observation group, n = 4 000) for 30 successive days (1 treatment course). The uncorrected visual acuity and diopter in each group were detected by an 'E' chart and optometry. Axial eye length and intraocular pressure in each group were measured using an ophthalmic A/B ultrasound scanner and a non-contact tonometer to assess the therapeutic effects. The incidence of adverse reactions during the treatment period was calculated. Results:After 30 days of treatment, the uncorrected visual acuity in the observation group was significantly higher than that in the control group [(0.88 ± 0.33) vs. (0.81 ± 0.29), t = 10.077, P < 0.001]. The diopter in the observation group was significantly lower than that in the control group [(-0.99 ± 0.36) D vs. (-1.07 ± 0.39) D, t = 9.533, P < 0.001]. Total effective rate in the observation group was significantly higher than that in the control group (91.10% vs. 88.18%, χ2 = 18.422, P < 0.001). There were no significant differences in the axial eye length, intraocular pressure and the incidence of adverse reactions between the two groups (all P > 0.05). Conclusion:Xiatianwu eye drops combined with raceanisodamine eye drops in the treatment of pseudomyopia in children can improve uncorrected visual acuity, increase therapeutic effects, and does not increase the incidence of adverse reactions.

4.
Article in Chinese | WPRIM | ID: wpr-885076

ABSTRACT

Objective:To evaluate the effects of vitamin K 2 on sevoflurane-induced cognitive decline in aged mice. Methods:A total of 72 SPF healthy female C57BL/6J mice, aged 12 months, weighing 20-25 g, were divided into 4 groups ( n=18 each) using a random number table method: control+ corn oil group (group Con+ Oil), sevoflurane+ corn oil group (group Sevo+ Oil), control+ vitamin K 2 group (group Con+ K 2) and sevoflurane+ vitamin K 2 group (group Sevo+ K 2). The mice in Sevo+ Oil and Sevo+ K 2 groups were anesthetized with 2.5% sevoflurane+ 33% oxygen for 2 h. The mice in Con+ Oil and Con+ K 2 groups were treated with 33% oxygen only.The animals in Con+ Oil and Sevo+ Oil groups were intraperitoneally injected with corn oil 100 μl at 30 min before oxygen or sevoflurane inhalation.Vitamin K 2 (dissolved in corn oil, concentration 1 mg/ml) 100 mg/kg was injected intraperitoneally in Con+ K 2 and Sevo+ K 2 groups.At 24 h after sevoflurane inhalation, 8 mice from each group were randomly selected and sacrificed, and the hippocampal tissues were removed for determination of activity of ATPase, contents of interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α) (by enzyme-linked immunosorbent assay) and the expression of AT8 and PHF1 (by Western blot). The remaining 10 mice in each group received standardized feeding, and the cognitive function was assessed using Y-maze at 1, 3, 5, 7 and 14 days after sevoflurane inhalation. Results:Compared with group Con+ Oil, the contents of IL-1β, IL-6 and TNF-α were significantly increased, expression of AT8 and PHF1 were up-regulated, activity of ATPase was decreased, and spontaneous alternation percentage was decreased at 1, 3, 5, 7 and 14 days after sevoflurane inhalation in group Sevo+ Oil ( P<0.05). Compared with group Sevo+ Oil, the contents of IL-1β, IL-6 and TNF-α were significantly decreased, expression of AT8 and PHF1 were down-regulated, activity of ATPase was increased, and spontaneous alternation percentage was increased at 1, 3, 5, 7 and 14 days in group Sevo+ K 2 ( P<0.05). There was no significant difference in the above indicators between group Con+ K 2 and group Sevo+ K 2 ( P>0.05). Conclusion:Vitamin K 2 can improve sevoflurane-induced cognitive decline in aged mice, the mechanism is related to increasing activity of ATPase and inhibiting the up-regulation of AT8 and PHF1 expression in hippocampus.

5.
Article in Chinese | WPRIM | ID: wpr-884823

ABSTRACT

Objective:Metabolic syndrome (MS) patients and determine factors related to platelet hyperactivation.Methods:A total of 999 participants (822 males and 177 females) were enrolled. Participants were divided into two groups according to the metabolic index: MS group (501) and normal control (NC) group (498). Platelet function was tested with PFA100 (Siemens USA, Deerfield, Illinois) which measures the time it takes for blood to occlude an aperture (closure time, CT) following stimulation with collagen and adenosine diphosphate. Statistical analyses were performed using the SPSS statistical software package, version 22.0 (IBM Corporation, Armonk, NY, USA).Results:MS group had significantly shorter CT values compared with NC group (106.6±27.8s) vs (113.7±27.9s). Higher BMI, WC, BP, TC, TG, LDL-C, UA, and PRL were correlated with shorter CT values (all P<0.05). Conclusion:Our results indicated platelet hyperactivation exists in MS which was related to obesity and elevated blood pressure, blood lipids, uric acid, and PLR.

6.
Article in Chinese | WPRIM | ID: wpr-870168

ABSTRACT

Objective:To investigate the significance of plasma pentraxin 3 (PTX3) in patients with secondary hemophagocytic lymphohistiocytosis (sHLH).Methods:Plasma PTX3 levels were tested by ELISA in 48 newly diagnosed sHLH patients, 18 healthy volunteers and 9 lymphoma controls in the First Affiliated Hospital of Nanjing Medical University from January 2017 to July 2019. Clinical parameters were collected, and the correlations with PTX3 levels were analyzed.Results:PTX3 level in newly diagnosed group was significantly higher than that of healthy control group [16.29(1.17-66.00) vs. 0.76(0.01-7.86) μg/L, P<0.01]. Patients with lymphoma-associated HLH(LHLH) had higher plasma level of PTX3 than Fhose with infection-associated HLH (IHLH) [24.29(3.36-66.00) vs. 9.56(1.17-36.50)μg/L, P<0.05]. Plasma PTX3 levels in 48 sHLH patients were positively correlated with serum ferritin ( P<0.05). Receiver operating characteristic (ROC) curve for plasma PTX3 levels of sHLH and healthy controls produced a cutoff value at 3.9 μg/L, with its 86.7% sensitivity and 94.4% specificity. And ROC analysis showed that PTX3 17.5 μg/L was the critical value for diagnosis of LHLH from non-LHLH group, that the sensitivity and specificity were 63.0% and 76.2% respectively. The 1-year overall survival (OS) rate in patients with PTX3≥17.5 μg/L was significantly lower in those with PTX3<17.5 μg/L (18.5% vs. 75.8%, P<0.01). Conclusion:These results indicate the potential of PTX3 as a biomarker for diagnosis and prognosis in patients with sHLH.

7.
Article in Chinese | WPRIM | ID: wpr-869783

ABSTRACT

Objective:To evaluate the effect of dexmedetomidine on intestinal barrier function in mice with traumatic brain injury (TBI) and the role of nuclear factor E2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway.Methods:Thirty ICR male wild-type (WT) and 30 Nrf2 knockout (Nrf2-KO) mice, aged 6-8 weeks, weighing 20-25 g, were divided into 3 groups ( n=10 each) using a random number table method: control group (group C), TBI group (group T) and TBI+ dexmedetomidine group (group T+ D). A 100 g of stainless steel impactor was used to produce a free fall acceleration strike on the head from 12 cm height in anesthetized animals.Dexmedetomidine 30 μg/kg was intraperitoneally injected at 30 min before TBI in group T+ D, while the equal volume of normal saline was given instead in C and TBI groups.The mouse bladder was emptied at 18 h after TBI, the test solution 200 μl containing 13.3 mg lactulose and 10.1 mg mannitol was given via a gastric tube.Urine was collected at 24 h after TBI, and the ratio of lactulose to mannitol was measured to estimate the intestinal barrier permeability.Blood samples were collected from heart to measure the concentration of lipopolysaccharide in plasma.Then the mice were sacrificed, and the tissues of ileum were collected to detect the contents of tumor necrosis factor-alpha(TNF-α), interleukin-1beta(IL-1β), IL-6 and 8-iso-prostaglandin F 2α (8-iso-PGF 2α) (by enzyme-linked immunosorbent assay), contents of superoxide dismutase (SOD) and catalase (CAT) (by hydroxylamine method and ammonium molybdate colorimetric method, respectively), content of malondialdehvde (MDA) (by thibabituric acid method), and expression of Nrf2 and HO-1 (by Western blot). Results:For WT mice Compared with group C, the intestinal barrier permeability, concentration of LPS in plasma, and contents of TNF-α, IL-1β, IL-6, MDA and 8-iso-PGF 2α were significantly increased, the activities of intestinal CAT and SOD were decreased, and the expression of Nrf2 and HO-1 was up-regulated in T and T+ D groups ( P<0.05). Compared with group T, the intestinal barrier permeability, concentration of LPS in plasma, and contents of TNF-α, IL-1β, IL-6, MDA and 8-iso-PGF 2α were significantly decreased, the activities of intestinal CAT and SOD were increased, and the expression of Nrf2 and HO-1 was up-regulated in group T+ D ( P<0.05). For Nrf2-KO mice Compared with group C, the intestinal barrier permeability, concentration of LPS in plasma, and contents of TNF-α, IL-1β, IL-6, MDA and 8-iso-PGF 2α were significantly increased, and no significant change was found in the activities of intestinal CAT and SOD in T and T+ D groups( P>0.05). There was no significant difference in the expression of HO-1 among the three groups ( P>0.05). Conclusion:Dexmedetomidine can improve the intestinal barrier dysfunction in mice with TBI, and the mechanism is related to activating Nrf2/HO-1 signaling pathway.

8.
Chinese Journal of Dermatology ; (12): 519-524, 2019.
Article in Chinese | WPRIM | ID: wpr-755792

ABSTRACT

Objective To compare the efficacy and safety of the long-term intermittent maintenance treatment with tacrolimus 0.03% ointment versus traditional treatment in reducing relapses and prolonging the recurrence interval in children with moderate to severe atopic dermatitis (AD).Methods A two-phase randomized,open-labelled,controlled clinical trial was conducted from September 2012 to November 2013.In the first phase,a total of 171 children aged 2-15 years with moderate to severe AD were enrolled from 7 hospitals in China,and received conventional treatment with tacrolimus 0.03% ointment twice a day for 2-6 weeks.At the end of the treatment,the patients who achieved an investigator's global assessment (IGA) score ≤ 2 (n =125) were randomly classified into 2 groups to receive the second-phase treatment:test group (n =62) receiving intermittent maintenance treatment with tacrolimus 0.03% ointment twice a week (Monday and Thursday),and control group (n =63) receiving no treatment.If the patients in the 2 groups experienced relapse,they received conventional treatment with tacrolimus 0.03% ointment twice a day.The overall observation period was 6 months.The primary endpoint was the time to the first relapse,which was defined as the number of days from the end of the first-phase treatment to the first relapse.The secondary endpoints included the number of relapses at the second-phase trial,the disease severity at the time of relapse,the duration of relapse,the pruritus score at the time of relapse,the total amount of tacrolimus ointment used,the total response rate at the second-phase trial,and the incidence of adverse events.Results A total of 125 children with AD were enrolled into the second-phase trial,and 121 of them completed the follow-up.Among the 121 patients,the recurrence rate was significantly lower in the test group (25/60,41.7%) than in the control group (46/61,75.4%;x2 =14.20,P < 0.001).The time to the first relapse was significantly longer in the test group (46.9 ± 37.7 d) than in the control group (28.8 ± 32.3 d;Z =1 093.50,P =0.020).The total number of recurrence was 31 and 86 in the test group and control group respectively,and the mean number of recurrence in each patient was significantly lower in the test group (0.52 ± 0.68) than in the control group (1.41 ± 1.23,t =4.96,P < 0.001).There were no significant differences between the two groups regarding disease severity during relapse (eczema area and severity index:Z =971.50,P =0.39),duration of relapse (Z =747.00,P =0.07),and pruritus score during relapse (Z =894.00,P =0.95).The therapeutic drug was tolerated well in all the children,and no tacrolimus-related serious adverse events occurred.Conclusion The intermittent maintenance treatment with tacrolimus 0.03% ointment twice a week for 6 months can effectively and safely prevent and reduce relapses,and prolong the recurrence interval in children with moderate to severe AD.

9.
Article in Chinese | WPRIM | ID: wpr-745239

ABSTRACT

Objective To investigate the effect of respiratory movement of different amplitude,period and direction on the dose distribution of target area in dynamic intensity modulated radiation therapy.Methods A total of 30 cases of lung cancer were selected and divided into three groups according to the volume size of the target area,including groups A (72.0-200.2 cm3),B (271.7-380.0 cm3) and C (498.9-684.9 cm3).The average volume was 151.5,327.1 and 583.3 cm3,respectively.Breathing motion simulation platform was used to drive the mode body with two-dimensional ionization chamber matrix along the Gun-Target direction,then turn the collimator to 0° and 90°,respectively.The doses were collected at the central level in different amplitudes of 0,4,8,12 and 15 mm,periodic respiratory movement at the intervals of 3,4 and 5 s and respiratory motion measurement with a cycle of 4 s 5 times.The difference of dose distribution between the collected dose and TPS output was analyzed by taking the absolute dose and γ-passing rate (3 mm/3%) as indicators.Results In the two-sided upward,respiratory movement reduced the dose at the medial edge of the target area and increased the dose at the lateral edge of the target area.The difference of γ-passing rate between respiration cycle was up to 3.54% (t=2.301,P<0.05),and when the respiration movement was more than 8 mm,the γ-passing rate was less than 90% and decreased with the increase of amplitude.The difference of γ-passing rate between static and respiratory motion was negatively correlated with the volume of target area,and the average γ-passing rate of A,B and C three groups increased gradually.The γ-passing rate of 5 composited dose was higher than that of single dose,and the difference was statistically sigificant(t=-9.36--5.95,P<0.05).Conclusions The dose distribution of dynamic IMRT target area is mainly influenced by respiration range and its own volume,and the respiration cycle has an effect on dose distribution under partial amplitude.After implementing the multiple doses,some single dose implementation errors can be eliminated.Physicians need to expand the target area reasonably according to the range of respiratory movement,and optimize the amount of marginal tissue in the target area in the direction of respiratory movement.For patients with small target volume and large respiratory movement,respiratory management technology should be adopted to improve the accuracy of target dose implementation.

10.
Article in Chinese | WPRIM | ID: wpr-744813

ABSTRACT

Objective To study the effects of mineral oil covered M2 culture medium droplet culture, M16 droplet culture, and dimethyl sulfoxide (DMSO) on the morphology and survival rates of mouse oocytes during the release from diplotene arrest. Methods Oocytes were randomly divided into 3 groups and individually cultured for 4 h in M2 covered with mineral oil, M16 covered with mineral oil, and/or M16 only to cause germinal vesicle breakdown (GVBD). The morphological changes and survival rates of oocytes in each group were observed under the microscope. Oocytes were randomly divided into 3 groups and cultured in the medium with 0%, 1%, and 2% DMSO. The effect of DMSO on oocytes was also observed during the release from diplotene arrest. Results The survival rates of oocytes in M2 covered with mineral oil were higher than those in M16 (P < 0.05). There was no statistical difference with respect to release of mouse oocytes from diplotene arrest between the oocytes in M2 covered with mineral oil and oocytes in M16. The shape of oocytes in M2 with mineral oil was better than that of oocytes in M16. The effect of DMSO on the survival rate of oocytes was similar in the medium with 0%, 1% and 2%DMSO. But the effect of 2% DMSO on the release of oocytes was statistically significant (P < 0.01). Conclusion During the release of mouse oocytes from diplotene arrest, oocytes in M2 covered with mineral oil have much better morphology and higher survival rate than those in M16. DMSO (0%, 1% and 2%) has no effect on the survival rate of oocytes. However, 2% DMSO is more effective in promoting the release of mouse oocytes from diplotene arrest.

11.
Chinese Journal of School Health ; (12): 1475-1477, 2019.
Article in Chinese | WPRIM | ID: wpr-815882

ABSTRACT

Objective@#To improve physical activity by assessing pedometer-based physical activity during specific intervals over a week, among junior high school students,and to provide a reference for improving the level of students’ physical activity.@*Methods@#Students (n=675) of grades 6-8 from six public junior high schools in Shanghai were recruited and instructed to wear a pedometer for a week, step counts and contents were recorded over a specific period. This period mainly included steps taken on the way to and from school, at school, at home in the evening on weekdays, and in the morning, afternoon, and evening on weekends.@*Results@#The daily step counts recorded were 8 332 steps, with those on weekdays showing significantly higher values than step counts on weekends (9 065 steps vs. 6 392 steps)(t=22.9, P<0.01). Proportionately, the physical activity level at school contributed more to daily step counts (61.3%), followed by those on the commute to and from school(25.5%). Boys were more active than girls. For all intervals on weekdays, the step counts of students in rural districts, with overweight or obese, in grades 6 and 7, were higher than those in urban districts, with normal status, in grade 8 respectively (P<0.05). Low-activity students with physical education were more active than one without physical education(t boy=1.99,t girl=2.45,P<0.05).@*Conclusion@#These findings facilitate the implementation of effective, feasible interventions to enhance physical activity over a series of intervals during the day.

12.
Chinese Journal of Hematology ; (12): 502-506, 2019.
Article in Chinese | WPRIM | ID: wpr-805561

ABSTRACT

Objective@#To investigate the clinical characteristics of secondary hemophagocytic lymphohistiocytosis (sHLH) complicated with capillary leak syndrome (CLS) .@*Methods@#The clinical and laboratory data of 87 sHLH patients, who were treated in our hospital between January 2015 and December 2017, were retrospectively analyzed. Depending on whether they were complicated with CLS, 21 sHLH patients were classified as the CLS-sHLH group, while 66 were classified as the non-CLS-sHLH group. The differences of clinical manifestations, laboratory tests, treatment and prognosis between the two groups were compared.@*Results@#There was no significant difference in the etiology of sHLH between the CLS-sHLH group and the non-CLS-sHLH group (P>0.05) . The neutrophil, fibrinogen and albumin levels in the CLS-sHLH group were lower than those in the non-CLS-sHLH group, while the triacylglycerol levels were higher than those in the non-CLS-sHLH group (P<0.05) . Varying degrees of edema, weight gain, hypotension, hypoproteinemia, oliguria and multiple serous effusions were observed in the CLS-sHLH group. Among them, there were 15 patients that CLS get improved, and the medial time of improvement was 7 (5-14) days. The other 6 patients did not get remission, while they died within 6-30 days. The median overall survival of the CLS-sHLH group was lower than that of the non-CLS-sHLH group (75 days vs not reached, P=0.031) .@*Conclusions@#There may be no correlation between the cause of sHLH and the occurrence of CLS. Severity of neutropenia, fibrinogen and albumin levels, and triglyceride levels may be accompanied for sHLH patients complicated with CLS. Patients with sHLH who complicated with CLS have a poor prognosis. Active treatment of HLH and its primary disease, reasonable fluid replacement and oxygen supply are crucial, which can effectively control disease progression.

13.
International Journal of Surgery ; (12): 686-691, 2019.
Article in Chinese | WPRIM | ID: wpr-797190

ABSTRACT

Objective@#To analysis the incidence and relevant clinical factors of colorectal polyps and adenomas in population of health examination.@*Methods@#Colonoscopy results and clinical data of 615 cases undergoing health examination from January 2018 to March 2019 were analyzed retrospectively in International Medical Center, Beijing Friendship Hospital, Capital Medical University.There were 436 males and 179 females, average age 47.38 years, aged 18-81 years. The clinical data contained sex, age, smoking history, drinking history, body mass index.Chi-square test and trend chi-square test were used to compare the differences of polyp detection rate and adenoma detection rate in different populations. Multivariate Logistic regression analysis were applied to explore the potential factors associated with the polyp detection rate and adenoma detection rate.@*Results@#Two hundred and forty cases(39.02%) with colorectal disease were checked out, include 206 cases(33.50%) with colorectal polyps and 138 cases(22.44%) with colorectal adenomas. In the detection rate of colorectal polyps, male were higher than female[36.70%(160/436) vs 25.70%(46/179), χ2=6.89, P<0.05], smokers were higher than non-smokers[42.35%(108/255) vs 27.22%(98/360), χ2=15.34, P<0.001], and drinkers were higher than non-drinkers[40.67%(109/268) vs 27.95%(97/347), χ2=10.98, P<0.05]. With the increase of age, the detection rate of colorectal polyps increased significantly(χ2=24.19, P<0.001). With the increase of body mass index, the detection rate of colorectal polyps increased significantly(χ2=16.88, P<0.001). In the detection rate of colorectal adenoma, smokers were higher than non-smokers[28.24%(72/255) vs 18.33%(66/360), χ2=7.31, P<0.05], and drinkers were higher than non-drinkers[27.61(74/268)% vs 18.44%(64/347), χ2=7.30, P<0.05]. With the increase of age, the detection rate of colorectal adenoma increased significantly(χ2=15.87, P<0.001). With the increase of body mass index, the detection rate of colorectal adenoma increased significantly (χ2=13.30, P<0.001). There was no significant difference in the detection rate of colorectal adenomas between male and female[24.31%(106/436) vs 17.88%(32/179), χ2=3.02, P>0.05]. Multivariate Logistic regression analysis showed that age increasing, body mass index increasing, smoking and alcohol consumption were risk factors for colorectal polyps and adenomas.@*Conclusions@#The detection rate of colorectal polyps and adenomas are related with sex, age, smoking, drinking and body mass index. Age increasing, body mass index increasing and smoking are the risk factors for colorectal polyps, age increasing and body mass index increasing are the risk factors for colorectal adenomas.

14.
International Journal of Surgery ; (12): 686-691, 2019.
Article in Chinese | WPRIM | ID: wpr-789135

ABSTRACT

Objective To analysis the incidence and relevant clinical factors of colorectal polyps and adenomas in population of health examination.Methods Colonoscopy results and clinical data of 615 cases undergoing health examination from January 2018 to March 2019 were analyzed retrospectively in International Medical Center,Beijing Friendship Hospital,Capital Medical University.There were 436 males and 179 females,average age 47.38 years,aged 18-81 years.The clinical data contained sex,age,smoking history,drinking history,body mass index.Chi-square test and trend chi-square test were used to compare the differences of polyp detection rate and adenoma detection rate in different populations.Multivariate Logistic regression analysis were applied to explore the potential factors associated with the polyp detection rate and adenoma detection rate.Results Two hundred and forty cases (39.02%) with colorectal disease were checked out,include 206 cases(33.50%) with colorectal polyps and 138 cases(22.44%) with colorectal adenomas.In the detection rate of colorectal polyps,male were higher than female [36.70% (160/436) vs 25.70% (46/179),x2 =6.89,P < 0.05],smokers were higher than non-smokers[42.35% (108/255) vs 27.22% (98/360),x2 =15.34,P <0.001],and drinkers were higher than non-drinkers[40.67% (109/268) vs 27.95% (97/347),x2 =10.98,P <0.05].With the increase of age,the detection rate of colorectal polyps increased significantly (x2 =24.19,P < 0.001).With the increase of body mass index,the detection rate of colorectal polyps increased significantly(x2 =16.88,P <0.001).In the detection rate of colorectal adenoma,smokers were higher than non-smokers [28.24% (72/255) vs 18.33 % (66/ 360),x2 =7.31,P < 0.05],and drinkers were higher than non-drinkers [27.61 (74/268)% vs 18.44% (64/ 347),x2 =7.30,P < 0.05].With the increase of age,the detection rate of colorectal adenoma increased significantly(x2 =15.87,P < 0.001).With the increase of body mass index,the detection rate of colorectal adenoma increased significantly (x2 =13.30,P < 0.001).There was no significant difference in the detection rate of colorectal adenomas between male and female [24.31% (106/436) vs 17.88% (32/179),x2 =3.02,P > 0.05].Multivariate Logistic regression analysis showed that age increasing,body mass index increasing,smoking and alcohol consumption were risk factors for colorectal polyps and adenomas.Conclusions The detection rate of colorectal polyps and adenomas are related with sex,age,smoking,drinking and body mass index.Age increasing,body mass index increasing and smoking are the risk factors for colorectal polyps,age increasing and body mass index increasing are the risk factors for colorectal adenomas.

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Article in Chinese | WPRIM | ID: wpr-708007

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Objective To investigate the dosimetric effect of carbon fiber couch through virtual simulation in the XiO treatment planning system (TPS).Methods A treatment couch model of iBEAM evo Extension 650 was scanned with a big bore spiral CT and its contour was stored in the XiO TPS.The attenuation coefficient of couch was obtained by measuring the attenuated dose with and without a solid water phantom on the couch at different gantry angles (100°-180°).The optimal relative electron density (RED) values of the carbon fiber (CF) cover and foam core (FC) were adjusted according to the comparison between measured and simulated attenuation dose.The effects of the couch in the TPS on pass rate were evaluated by Octavius 4D phantom with 10 cases with lung cancer.Results The optimal RED values of CF and FC were 0.75 and 0.10 g/cm3,respectively.The measured attenuation error was the maximal at gantry angle of 120° (4.84%) without the treatment couch in the TPS.The average measured attenuation errors without the couch in the TPS dropped significantly from (2.54 ± 1.48) % to (-0.04 ± 0.36) % after inclusion of the treatment couch during dose calculation (Z =-3.621,P < 0.05).The three-dimensional dose verification γ pass rate (3 mm/3%) without the couch increased significantly from (91.79± 1.25)% to (94.74± 1.69)% after inclusion of the couch in the dose calculation (t =6.027,P < 0.05).Conclusions The effect of couch on the attenuation dose is significant.Inclusion of a virtual model of couch in XiO TPS can simulate the attenuation effect properly and improve the accuracy of dose calculation.

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Article in Chinese | WPRIM | ID: wpr-704161

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Objective To evaluate the impact of Montessori-based intervention on eating ability of the elderly with dementia.Methods Sixty-four patients with dementia were randomly divided into intervention group(n=32) and control group(n=32).Patients in intervention group were given Montessori-based intervention,and patients in control group received regular care.The effect of Montessori-based intervention on eating ability,eating difficulty and self-eating time were assessed by the simplified Chinese version of EBS (C-EBS),simplified Chinese version of Edinburgh feeding evaluation in dementia scale (C-EdFED) and stopwatch respectively at pre-intervention,post-intervention,1-month and 3-month follow-up.Results Compared with the control group (EBS:(12.42± 3.59);EdFED:(10.48± 3.83);self-eating time:(15.28±6.04) min)),the average scores of EBS(14.31±2.63) increased and the self-eating time ((21.44±7.17)min) increased after 8 weeks intervention in intervention group,while the average scores of EdFED (7.86±4.16) increased.The C-EBS scores and self-eating time in intervention group were significantly higher than that of control group while the C-EdFED scores were lower than that of control at all time points(P<0.01).The difference in time effect between the two groups was statistically significant (P < 0.05)Conclusion The Montessori-based intervention can improve the eating ability of elderly people with dementia,reduce eating difficulty and increase self-eating time.

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Cancer Research and Clinic ; (6): 678-681, 2018.
Article in Chinese | WPRIM | ID: wpr-712883

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Objective To investigate clinicopathological significance of atypical squamous epithelium cells which cannot exclude high grade squamous intraepithelial lesions (ASC-H) in the diagnosis of cervical diseases. Methods The results of age, high-risk human papillomavirus DNA (hrHPV DNA) and cervical biopsy in 496 patients with ASC-H from March 2012 to December 2015 in Shanxi Dayi Hospital were analyzed. Results Among 496 ASC-H cases, the proportion of the patients between 40 and 49 years old was the highest [30.8 % (153/496)]. HrHPV DNA was detected in 154 cases, and the positive rate was 79.2 %(122/154), and the positive rate of patients at the age of 18 to 29 years old was the highest [84.2 % (16/19)]. The detection rate of cervical intraepithelial neoplasia (CIN) Ⅱand above lesions in 124 cases with cervical biopsy was 66.9 % (83/124), including 100 cases with hrHPV DNA positive (80.6 %) and 24 cases with hrHPV DNA negative (19.4 %). There were 71 cases (71.0 %) of CINⅡ, CIN Ⅲ, early squamous carcinoma and squamous cell carcinomas in 100 hrHPV DNA positive patients with cervical biopsy. There were 12 cases (50.0 %) of CINⅡ/Ⅲ changes in 24 hrHPV DNA negative patients with cervical biopsy, but none in early squamous carcinoma and squamous cell carcinomas was detected, and there was a significant difference between hrHPV DNA positive and negative patients (χ2=3.857, P< 0.05). The positive predictive value, negative predictive value, sensitivity and specificity of hrHPV DNA detection for diagnosis of CINⅡand above lesions were 85.5 %, 29.3 %, 71.0 % and 50.0 %, respectively. Conclusions ASC-H strongly predicts CINⅡand above lesions in cervical cytology. The detection of hrHPV DNA has a high positive predictive value for CINⅡand above lesions.

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Article in Chinese | WPRIM | ID: wpr-710332

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Objective To investigate the role of mast cells in Staphylococcus aureus enterotoxin B (SEB)-induced atopic dermatitis (AD)-like skin inflammation in BALB/c mice.Methods A total of 24 BALB/c mice were randomly and equally divided into 4 groups to be topically treated with ovalbumin (OVA group),SEB (SEB group),OVA + SEB (OVA + SEB group) and sodium chloride physiological solution (control group) respectively,so as to establish mouse models of epicutaneously induced AD-like skin inflammation.The AD-like skin lesions were evaluated by clinical observation and eczema area and severity index (EASI).Biopsy specimens were obtained from lesional skin of mice and then subjected to toluidine blue staining and immunohistochemical staining to count the mast cells,observe the morphology and distribution of mast cells,and calculate the percentage of degranulated mast cells.Results After 7-week treatment,the OVA group,SEB group and OVA + SEB group all showed severer local skin inflammation,higher EASI scores and denser infiltration of inflammatory cells compared with the control group.Moreover,the OVA + SEB group showed significantly severer local skin inflammation,skin lesions and degree of infiltration of inflammatory cells compared with the OVA group and SEB group (all P < 0.05).The number of mast cells in the dermis of AD-like skin lesions per high-power field (× 400) was significantly higher in the OVA group (median [quartile range]:10.625 [3.675]),SEB group (11.000 [4.163]) and OVA + SEB group (13.875 [8.813]) than that in the control group (5.925 [2.088],all P < 0.05).The SEB group (71.083% ± 14.519%) and OVA + SEB group (58.767% ±.16.978%) both showed significantly higher percentage of degranulated mast cells compared with the OVA group (24.050% ± 11.161%,both P < 0.05) and control group (23.617% ± 8.132%,both P < 0.05).Bivariate correlation analysis showed that the number of mast cells in the skin lesions was positively linearly correlated with the EASI scores (P < 0.05).Conclusions Epicutaneous application of SEB can induce AD-like skin lesions in mice,and can exacerbate the severity of OVA-induced AD-like skin lesions.Mast cell proliferation,activation/degranulation and tryptase release may participate in the inflammation.

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Article in Chinese | WPRIM | ID: wpr-709699

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Objective To evaluate the effect of hydrogen on nuclear factor erythroid 2?related factor 2(Nrf2)∕heme oxygenase?1(Nrf2∕HO?1)signaling pathway in brain tissues of mice with sepsis?associat?ed encephalopathy. Methods Ninety adult male ICR mice, weighing 20-25 g, were divided into 3 groups(n=30 each)using a random number table: sham operation group(group SH), sepsis?associated encephalopathy group(group SAE)and sepsis?associated encephalopathy plus hydrogen group(group SAE+H). Sepsis was produced by cecum ligation and puncture(CLP). Hydrogen?rich normal saline 10 ml∕kg was injected intraperitoneally at 1 and 3 h after CLP in group SAE+H. At 24 h after CLP, tissues in hippocampal CA1 area were obtained for calculation of the density of dendritic spine(using Golgi staining) and for microscopic examination of apoptosis in nerve cells(by TUNEL). Apoptotic index(AI)was cal?culated. At 6, 12 and 24 h after CLP, the expression of Nrf2 in hippocampal nucleoprotein(N?Nrf2), HO?1 in total protein and high?mobility group box 1 protein(HMGB1)was detected by Western blot, and the expression of HMGB1 mRNA was determined by real?time polymerase chain reaction. Results Com?pared with group SH, the density of dendritic spines and AI in hippocampal CA1 area were significantly in?creased, and the expression of hippocampal N?Nrf2, HO?1, HMGB1 protein and mRNA was up?regulated in SAE and SAE+H groups(P<0.05). Compared with group SAE, the density of dendritic spines and AI in hippocampal CA1 area were significantly decreased, the expression of hippocampal N?Nrf2 and HO?1 was up?regulated, and the expression of HMGB1 protein and mRNA was down?regulated in group SAE+H(P<0.05). Conclusion The mechanism by which hydrogen reduces sepsis?associated encephalopathy may be related to activating Nrf2∕HO?1 signaling pathway in brain tissues of mice.

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Article in Chinese | WPRIM | ID: wpr-807826

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Objective@#To observe the clinical effects and safety of orthokeratology(OK) lens in the treatment of mild and moderate myopia, and to evaluate the effects on the myopia control.@*Methods@#The study included a total of 166 eyes in 83 myopia children who were treated in Jiaxing Traditional Chinese Medicine Hospital Affiliated to Zhejiang Chinese Medical University from January 2014 to December 2014.They were randomly divided into two groups according to the digital table, 43 cases(86 eyes) in the observation group underwent standard OK lens, and the other 40 cases(80 eyes) in the control group were given common spectacles.The patients were followed up for two years.The uncorrected visual acuity, corneal curvature and ocular surface in the, observation group were examined, and diopter, axial length were examined in the two groups.@*Results@#The visual acuity of the observation group improved significantly at 1 day, trended to be stable at 1 week, and then was basically stable within 1 year, but decreased at 2 years.After 3 months, the mean corneal curvature[(41.93±1.12)D]was significantly lower than before orthokeratology[(43.56±1.44)D], and the difference was statistically significant(t=11.539, P<0.05). However, the effect didn’t persist without continued OK lens wear 1 month later.Compared with the control group, the growth of the diopter and axial length in the observation group were significantly slower(t=16.784, P<0.05; t=5.623, P<0.05). At the initial wearing, 34% of the observation group occurred corneal epithelial injury, but they all recovered by stop wearing and appropriate medication.With the prolong of wear time, the proportion of injury reduced.@*Conclusion@#Uncorrected visual acuity of mild and moderate myopia children wearing OK lens can significantly improve at 1 day, then is basically stable after 1 year, but declines within 2 years, which indicates the need for timely replacement of the lens.Long-term wearing is effective and safe to control the development of myopia.

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