Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 18 de 18
Filter
1.
Clinics ; 76: e2690, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278911

ABSTRACT

This study aimed to explore the relationship between plasma interleukin 6 (IL-6) levels, adverse cardiovascular events, and the severity of acute coronary syndrome (ACS). A literature review was performed of studies regarding IL-6 and ACS extracted from databases including EMBASE, Cqvip, MEDLINE, Web of Knowledge, PubMed, Cochrane Library, China National Knowledge Infrastructure, and Wanfang data. The Newcastle-Ottawa scale (NOS) was used to evaluate the quality of the literature. The literature was screened, its quality was evaluated, and relevant data were extracted for performing meta-analysis using RevMan software (version 5.3). A total of 524 studies were included in the initial survey. After several rounds of screening and analysis, six studies met the inclusion criteria and underwent meta-analysis using a fixed-effect model. Patients were divided into non-severe and severe groups based on the concentration of high-sensitivity C-reactive protein. Meta-analysis of the relationship between IL-6 and the severity of ACS showed that the plasma IL-6 level of patients in the severe group was significantly higher than that of patients in the non-severe group (p<0.00001). Additionally, patients with experience of major adverse cardiovascular events had significantly higher plasma IL-6 levels than did patients without experience of such events (p<0.00001). In summary, patients with ACS and high IL-6 levels tended to be in a critical condition, with a higher risk of adverse cardiovascular events and worse prognosis. Thus, IL-6 levels could indicate whether patients with ACS may have adverse cardiovascular events and determine the severity of ACS.


Subject(s)
Humans , Interleukin-6 , Acute Coronary Syndrome , Prognosis , C-Reactive Protein , China
2.
Braz. arch. biol. technol ; 64: e21200316, 2021. tab, graf
Article in English | LILACS | ID: biblio-1278451

ABSTRACT

Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.


Subject(s)
Plants, Genetically Modified , Arabidopsis , Fragaria , Herbicides/adverse effects
3.
Braz. j. med. biol. res ; 54(7): e10213, 2021. tab, graf
Article in English | LILACS | ID: biblio-1249312

ABSTRACT

Sevoflurane (SEVO) is widely applied as an anesthetic, which exerts antitumor capacity in various cancers, including hepatocellular carcinoma (HCC). Previous studies indicated that long non-coding RNA KCNQ1 opposite strand/antisense transcript 1 (KCNQ1OT1) was upregulated, while microRNA-29a-3p (miR-29a-3p) was downregulated in HCC. Thus, we aimed to explore the roles of KCNQ1OT1 and miR-29a-3p in HCC cells exposed to SEVO. Cell proliferation, apoptosis, migration, and invasion were assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, flow cytometry, and transwell assays, respectively. The levels of genes were determined by quantitative real-time polymerase chain reaction (qRT-PCR) or western blot. Furthermore, the interaction between miR-29a-3p and KCNQ1OT1 or chromebox protein homolog 3 (CBX3) was predicted by Starbase or Targetscan, and then confirmed by dual-luciferase reporter assay. We found that the levels of KCNQ1OT1 and CBX3 were decreased, while miR-29a-3p was increased in SEVO-treated HCC cells. KCNQ1OT1 overexpression weakened the inhibitory effects of SEVO on HCC cell proliferation, apoptosis, migration, and invasion. Interestingly, KCNQ1OT1 bound to miR-29a-3p, and miR-29a-3p targeted CBX3. KCNQ1OT1 upregulated CBX3 level by repressing miR-29a-3p expression. Furthermore, KCNQ1OT1 exerted tumor promotion in HCC cells via suppressing miR-29a-3p to regulate CBX3 expression. Collectively, our findings demonstrated that KCNQ1OT1 regulated the antitumor effects of SEVO on HCC cells through modulating the miR-29a-3p/CBX3 axis, providing a theoretical basis for the treatment of HCC.


Subject(s)
Humans , Carcinoma, Hepatocellular/genetics , Carcinoma, Hepatocellular/drug therapy , Potassium Channels, Voltage-Gated , MicroRNAs/genetics , Liver Neoplasms/genetics , Liver Neoplasms/drug therapy , Chromosomal Proteins, Non-Histone , RNA, Long Noncoding/genetics , Sevoflurane/pharmacology
4.
J Cancer Res Ther ; 2020 Jan; 15(6): 1597-1602
Article | IMSEAR | ID: sea-213576

ABSTRACT

Aims: The purpose of this study was to investigate the potential correlation between metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and the transcription factor BTB and CNC homology 1 (BACH1) and their clinicopathological significance in triple-negative breast cancer (TNBC). Subjects and Methods: MALAT1 and BACH1 were detected by immunohistochemistry using TNBC tissue microarrays of 240 patients. The association between MALAT1 and BACH1 expression levels was statistically analyzed. Moreover, the prognostic roles as well as clinical and pathological significance of MALAT1 and BACH1 expression in TNBC were determined. Statistical Analysis Used: Two-tailed Pearson correlation was used to examine the correlation of BACH1 and MALA1 expression. Comparisons of clinicopathological variables between different BACH1 and MALA1 expression groups were performed using χ2 tests. Overall survival (OS) and disease-free survival (DFS) curves were plotted with the Kaplan-Meier method and the differences in OS and DFS between three groups were compared by the log-rank test. Multiple comparisons were performed using χ2 tests for subsequent individual group comparisons. Results: MALAT1 and BACH1 expression was significantly correlated with tumor-node-metastasis stage, distant metastasis, pathological stage, and survival outcomes of patients. Patients with high MALAT1 and BACH1 expression exhibited shorter overall survival and disease-free survival. Conclusions: These findings provide further insight into the expression pattern of MALAT1 and BACH1 in TNBC and suggest them as prognostic biomarkers for TNBC

5.
Chinese Journal of School Health ; (12): 580-582, 2020.
Article in Chinese | WPRIM | ID: wpr-821432

ABSTRACT

Objective@#To analyze the epidemiological characteristics and influencing factors of norovirus aggregation in primary and secondary schools in Haidian District of Beijing from 2015 to 2018,and to provide scientific basis for the prevention and control of school aggregation epidemic situation.@*Methods@#An epidemiological method was used to analyze the epidemic characteristics of norovirus aggregation in the primary and secondary schools in Haidian District of Beijing from January 2015 to December 2018. Multivariate Logistic was used to analyze the influencing factors of norovirus aggregation.@*Results@#A total of 74 cases of norovirus aggregation in primary and secondary schools in Haidian District of Beijing from 2015 to 2018 were reported, involving 52 primary and secondary schools. Totally 1 291 suspected cases and a median of 12.5 cases of single epidemic were reported. Winter and spring were high incidence season. There was significant difference in the incidence rate of epidemic situation in primary and secondary schools(χ2=98.82, P<0.01). There was significant difference in the incidence rate between men and women(χ2=55.56, P<0.01). A total of 648 samples and 486 positive samples were collected, with a positive rate of 75.00%, mainly GⅡ virus, accounting for 87.45%. Multivariate Logistic regression showed that the scale of school(>2 000,1 000~2 000), student’s hand hygiene habit,requirement for medical care after the outbreak is the influence factor of the outbreak(OR=3.78,2.95,8.25,2.88).@*Conclusion@#Poor hand hygiene habits are the most important risk factors for the outbreak, so schools and parents should cultivate children to develop good hygiene habits of washing hands before and after meals, and timely isolation of cases found in schools is also an important measure to prevent and control the epidemic.

6.
Braz. j. med. biol. res ; 52(11): e8333, 2019. tab, graf
Article in English | LILACS | ID: biblio-1039264

ABSTRACT

Not much is known about the roles of long non-coding RNAs (lncRNAs) for chronic kidney disease (CKD). In this study, we included CKD patient cohorts and normal controls as a discovery cohort to identify putative lncRNA biomarkers associated with CKD. We first compared the lncRNA expression profiles of CKD patients with normal controls, and identified differentially expressed lncRNAs and mRNAs. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs was constructed using WGCNA to identify important modules related to CKD. A lncRNA-miRNA-mRNA pathway network based on the hub lncRNAs and mRNAs, related miRNAs, and overlapping pathways was further constructed to reveal putative biomarkers. A total of 821 significantly differentially expressed mRNAs and lncRNAs were screened between CKD and control samples, which were enriched in nine modules using weighted correlation network analysis (WGCNA), especially brown and yellow modules. Co-expression network based on the enriched differentially expressed mRNAs and lncRNAs in brown and yellow modules uncovered 7 hub lncRNAs and 53 hub mRNAs. A lncRNA-miRNA-mRNA pathway network further revealed that lncRNAs of HCP5 and NOP14-AS1 and genes of CCND2, COL3A1, COL4A1, and RAC2 were significantly correlated with CKD. The lncRNAs of NOP14-AS1 and HCP5 were potential prognostic biomarkers for predicting the risk of CKD.


Subject(s)
Humans , RNA, Messenger/genetics , Genetic Markers/genetics , Renal Insufficiency, Chronic/genetics , RNA, Long Noncoding/genetics , Prognosis , Case-Control Studies , Mass Screening , Gene Expression Profiling , Renal Insufficiency, Chronic/diagnosis
7.
Article in Chinese | WPRIM | ID: wpr-753119

ABSTRACT

Objective : To analyze influencing factors of aortic pulse wave velocity (APWV) in patients with essential hypertension (EH).Methods : The 336 EH patients from our hospital were divided into elevated APWV group (n=281) and normal APWV group (n=55)- Clinical data were compared between two groups ,and multi-factor Logis-tic regression was used to analyze influencing factors of APWV in EH patients .Results : Compared with normal AP-WV group ,there were significant rise in age [ (56-71 ± 11-45) years vs-(62-98 ± 12-36) years] ,percentages of di-abetes mellitus (5-45% vs- 19-22%) ,obesity (18-18% vs- 23-84%) ,smoking (16-36% vs- 30-60%) , EH stage 3 (12-73% vs- 32-03%) ,body mass index [BMI , (22-53 ± 2-07) kg/m2 vs-(23-28 ± 2-04) kg/m2 ] ,systolic blood pressure SBP [ (139-64 ± 12-85) mmHg vs.(147-39 ± 13-30 ) mmHg] ,diastolic blood pressure DBP [ (93-18 ± 8-73) mmHg vs .(96-43 ± 9-14 ) mmHg] , TC [ (5-18 ± 0-91 ) mmol/L vs-(5-65 ± 1-03 ) mmol/L ] , LDL-C [ (3-00 ± 0-68 ) mmol/L vs-(3-24 ± 0-72 ) mmol/L ] ,serum creatinine SCr [ (110-71 ± 52-39 ) μmol/L vs-(138-52 ± 63-65) μmol/L] ,serum uric acid SUA [ (318-73 ± 102-62) μmol/L vs-(354-28 ± 108-35) μmol/L] , and significant reduction in level of HDL-C [ (1-19 ± 0-32) mmol/Lvs-(1-09 ± 0-30) mmol/L] in elevated AP-WV group , P<0-05 or <0-01- Multi-factor Logistic regression analysis indicated that age ,diabetes mellitus ,SBP , TC ,SCr and EH stage were independent risk factors for APWV (OR=2-826 -3-732 , P<0-05 all).Conclusion :Age ,diabetes mellitus ,SBP ,TC ,SCr and EH stage are risk factors for APWV in EH patients- Therefore ,corre- sponding preventive and therapeutic measures should be taken to improve prognosis .

8.
Article in English | WPRIM | ID: wpr-812415

ABSTRACT

Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood-brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.


Subject(s)
Administration, Oral , Animals , Antibodies , Blood-Brain Barrier , Metabolism , Brain , Metabolism , Brain Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Metabolism , Ginsenosides , Metabolism , Male , Mice , Mice, Inbred C57BL , Panax , Chemistry , Rats , Rats, Sprague-Dawley , Tandem Mass Spectrometry
9.
Article in English | WPRIM | ID: wpr-773625

ABSTRACT

Given the limited studies and conflicting findings, the transport character of ginsenosides crossing the blood-brain barrier (BBB) remains unclear. The present study was designed to qualitatively determine the distribution of ginsenosides in brain tissues after oral administration of ginseng total saponins, using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) combined with immunohistochemistry. In brain tissue homogenates, ginsenoside Rg1 was detectable and no other ginsenosides or their metabolites were found. No ginsenosides were detected in cerebrospinal fluid. Immunohistochemistry staining of brain tissue sections by using anti-ginsenoside polyclonal antibodies revealed the localization of ginsenosides in brain tissues. Furthermore, immunofluorescence double staining revealed that ginsenosides widely existed in vascular endotheliocytes and astrocytes, and in few neurons. These results indicated that Rg1 was the main component that entered the brain after oral administration of ginseng total saponins and that ginsenosides could cross the BBB, although the transport capability of ginsenosides through the BBB may be poor.


Subject(s)
Administration, Oral , Animals , Antibodies , Blood-Brain Barrier , Metabolism , Brain , Metabolism , Brain Chemistry , Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Metabolism , Ginsenosides , Metabolism , Male , Mice , Mice, Inbred C57BL , Panax , Chemistry , Rats , Rats, Sprague-Dawley , Tandem Mass Spectrometry
10.
Article in Chinese | WPRIM | ID: wpr-699362

ABSTRACT

Objective:To explore serum level of high sensitive C reactive protein(hsCRP)and expression of CX3C chemokine receptor 1(CX3CR1)on peripheral blood mononuclear cells(PBMC)in patients with coronary heart disease(CHD)and its clinical significance.Methods:A total of 153 CHD patients treated in our hospital from Mar 2016 to Mar 2017 were selected,including 50 cases with stable angina pectoris(SAP),52 cases with unstable angina pectoris(UAP)and 51 cases with acute myocardial infarction(AMI);according to coronary lesion degree assessed by Gensini score,153 CHD patients were divided into mild coronary disease group(n=43),moderate coronary dis-ease group(n=56)and severe coronary disease group(n=54).Another 45 healthy subjects were selected as healthy control group during the same period.Serum hsCRP level and CX3CR1 expression on PBMC were measured and compared among all groups,the correlation among serum hsCRP level,CX3CR1 expression and Gensini score were analyzed.Results:Compared with healthy control group,there were significant rise in serum hsCRP level[(2.36 ± 1.67)mg/L vs.(5.07 ± 2.16)mg/L vs.(13.59 ± 5.23)mg/L vs.(27.46 ± 8.24)mg/L]and CX3CR1 expression on PBMC[(0.48 ± 0.25)% vs.(11.13 ± 5.42)% vs.(20.56 ± 9.73)% vs.(37.49 ± 12.82)%]in SAP group,UAP group and AMI group,and AMI group> UAP group> SAP group,there was significant difference between any two groups,P=0.001 all.Compared with mild coronary disease group,there were significant rise in serum hsCRP level[(6.31 ± 1.83)mg/L vs.(17.42 ± 6.58)mg/L vs.(35.26 ± 9.74)mg/L]and CX3CR1 expression on PBMC [(6.59 ± 1.43)% vs.(25.86 ± 9.37)% vs.(42.57 ± 13.28)%]in moderate and severe coronary disease group,and those of severe coronary disease group were significantly higher than those of moderate coronary disease group,P=0.001 all.Linear correlation analysis indicated that serum hsCRP level and CX 3CR1 expression on PBMC were sig-nificantly correlated with Gensini score(r=0.768,0.897,P=0.001 both).Conclusion:Serum hsCRP level and CX3CR1 expression significantly rise in CHD patients along with the aggregation of coronary disease,which are ex-pected to be reference indexes predicting severity of CHD.

11.
Article in Chinese | WPRIM | ID: wpr-699359

ABSTRACT

Myocardial bridge is a kind of congenital structural variation, which is usually treated as benign lesions, but it can also cause myocardial ischemia, acute coronary syndrome, ventricular stunning and arrhythmias, even cardiogenic sudden death. The present article made a review on incidence rate, anatomical typing, pathophysiology, clinical manifestations, diagnosis and treatment of myocardial bridge.

12.
Article in Chinese | WPRIM | ID: wpr-666556

ABSTRACT

OBJECTIVE To investigate the effects of LW-AFC, a new formula derived from Liuwei Dihuang decoction, on gut microbiota and the behavior of learning and memory of SAMP8 mice, a mouse model of Alzheimer Disease (AD), and identify the specific intestinal microbiota correlating with cognitive ability. METHODS Morris-water maze test, novel object recognition test and shuttle-box test were conducted to observe the ability of learning and memory. 16S rRNA amplicon sequencing (Illumina, San Diego, CA, USA) was employed to investigate gut microbiota. RESULTS The treatment of LW- AFC improved cognitive impairments of SAMP8 mice, including spatial learning and memory ability, active avoidance response, and object recognition memory capability. Our data indicated that there were significantly 8 increased and 12 decreased operational taxonomic units (OTUs) in the gut microbiota of SAMP8 mice compared with senescence accelerated mouse resistant 1 (SAMR1) strains, the control of SAMP8 mice. The treatment of LW- AFC altered 22 (16 increased and 6 decreased) OTUs in SAMP8 mice and among them, 15 OTUs could be reversed by LW-AFC treatment resulting in a microbial composition similar to that of SAMR1 mice. We further showed that there were 7 (3 negative and 4 positive correlation) OTUs significantly correlated with all the three types of cognitive abilities, at the order level, including Bacteroidales, Clostridiales, Desulfovibrionales, CW040, and two unclassified orders. LW-AFC had influences on bacterial taxa correlated with the abilities of learning and memory in SAMP8 mice and restored them to SAMR1 mice. CONCLUSION The effects of LW-AFC on improving cognitive impairments of SAMP8 mice might be via modulating intestinal microbiome and LW-AFC could be used as a potential anti-AD agent.

13.
Yonsei Medical Journal ; : 735-740, 2016.
Article in English | WPRIM | ID: wpr-21839

ABSTRACT

PURPOSE: The aim of this study was to determine the relationship of hypoxia-inducible factor-2 (HIF-2α) and vascular endothelial growth factor (VEGF) with radiographic severity in primary osteoarthritis (OA) of the knee. Expression of these two factors in cartilage samples from OA knee joints was examined at mRNA and protein levels. MATERIALS AND METHODS: Knee joints were examined using plain radiographs, and OA severity was assessed using the Kellgren and Lawrence (KL) grading system. Specimens were collected from 29 patients (31 knees) who underwent total knee replacement because of severe medial OA of the knee (KL grades 3 and 4), 16 patients who underwent knee arthroscopy (KL grade 2), and 5 patients with traumatic knees (KL grade 0). HIF-2α and VEGF expression was quantified by real-time polymerase chain reaction and western blotting. RESULTS: Cartilage degeneration correlated with the radiographic severity grade. OA severity, determined using the Mankin scale, correlated positively with the KL grade (r=0.8790, p<0.01), and HIF-2α and VEGF levels with the radiographic severity of knee OA (r=0.7001, p<0.05; r=0.6647, p<0.05). CONCLUSION: In OA cartilage, HIF-2α and VEGF mRNA and protein levels were significantly and positively correlated. The expression of both factors correlated positively with the KL grade. HIF-2α and VEGF, therefore, may serve as biochemical markers as well as potential therapeutic targets in knee OA.


Subject(s)
Adult , Aged , Arthroplasty, Replacement, Knee , Arthroscopy , Basic Helix-Loop-Helix Transcription Factors/metabolism , Biomarkers/blood , Cartilage/metabolism , Female , Humans , Knee Joint/diagnostic imaging , Male , Middle Aged , Osteoarthritis, Knee/blood , RNA, Messenger , Radiography , Real-Time Polymerase Chain Reaction , Severity of Illness Index , Vascular Endothelial Growth Factor A/metabolism
14.
Rev. bras. farmacogn ; 25(5): 465-472, Sept.-Oct. 2015. tab, graf
Article in English | LILACS-Express | LILACS | ID: lil-765072

ABSTRACT

ABSTRACTIn this study, ten trace elements in Ziziphora clinopodioidesLam., Lamiaceae, from different regions, periods and parts in Xinjiang were determined by atomic absorption spectrometry following microwave-assisted acid digestion. The decreasing sequence of elements levels was K > Ca > Mg > Fe > Cu > Zn > Na > Mn > Cd > Pb. Chemometric approaches, such as correlation analysis, principal component analysis, and hierarchical cluster analysis were applied to classify Z. clinopodioides according to its elements contents. Principal component analysis revealed 83.51% of the variance with the first four principal component variables. Hierarchical cluster analysis indicated five groups from the eighteen regions, and the result of classification can correspond to the geographical distribution for the most regions. Variation in the elements exhibited a decreasing trend, but of different types in the studied periods. Elemental contents distributed in leaves were higher than those in flowers and stems. Therefore, chemometric approaches could be used to analyze data to accurately classify Z. clinopodioides according to origins. This study provided some elemental information on chemotaxonomy, diversity, changing pattern, distribution, and metabolism of Z. clinopodioides at spatial and temporal levels, and could be used as a reference of planting and quality standards.

15.
Article in English | WPRIM | ID: wpr-812190

ABSTRACT

AIM@#Variation in structure-related components in plant products prompted the trend to establish methods, using multiple or total analog analysis, for their effective quality control. However, the general use of routine quality control is restricted by the limited availability of reference substances. Using an easily available single marker as a reference standard to determine multiple or total analogs should be a practical option.@*METHOD@#In this study, the Ultra-HPLC method was used for the baseline separation of the main components in ginseng extracts. Using a plant chemical component database, ginsenosides in ginseng extracts were identified by Ultra-HPLC-MS analysis. The charged aerosol detection (CAD) system with post-column compensation of the gradient generates a similar response for identical amounts of different analytes, and thus, the content of each ginsenoside in ginseng extracts was determined by comparing the analyte peak area with the reference standard (determination of total analogs by single marker, DTSM). The total ginsenoside content was determined by the summation of reference standard and other ginsenoside components.@*RESULTS@#The results showed that DTSM approaches were available for the determination of total ginsenosides in a high purity ginseng extract because of the removal of impurities. In contrast, DTSM approaches might be suitable for determination of multiple ginsenosides without interference from impurities in the crude ginseng extract.@*CONCLUSION@#Future practical studies similar to the present study should be conducted to verify that DTSM approaches based on CAD with post-column inverse gradient for uniform response are ideal for the quality control of plant products.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Ginsenosides , Mass Spectrometry , Panax , Chemistry , Reference Standards
16.
Braz. j. med. biol. res ; 44(12): 1231-1242, Dec. 2011. ilus, tab
Article in English | LILACS | ID: lil-606547

ABSTRACT

The mitogenic effects of periodic mechanical stress on chondrocytes have been studied extensively but the mechanisms whereby chondrocytes sense and respond to periodic mechanical stress remain a matter of debate. We explored the signal transduction pathways of chondrocyte proliferation and matrix synthesis under periodic mechanical stress. In particular, we sought to identify the role of the MEK1/2-ERK1/2 signaling pathway in chondrocyte proliferation and matrix synthesis following cyclic physiologic mechanical compression. Under periodic mechanical stress, both rat chondrocyte proliferation and matrix synthesis were significantly increased (P < 0.05) and were associated with increases in the phosphorylation of Src, PLCγ1, MEK1/2, and ERK1/2 (P < 0.05). Pretreatment with the MEK1/2-ERK1/2 selective inhibitor, PD98059, and shRNA targeted to ERK1/2 reduced periodic mechanical stress-induced chondrocyte proliferation and matrix synthesis (P < 0.05), while the phosphorylation levels of Src-Tyr418 and PLCγ1-Tyr783 were not inhibited. Proliferation, matrix synthesis and phosphorylation of MEK1/2-Ser217/221 and ERK1/2-Thr202/Tyr204 were inhibited after pretreatment with the PLCγ1 inhibitor U73122 in chondrocytes in response to periodic mechanical stress (P < 0.05), while the phosphorylation site of Src-Tyr418 was not affected. Inhibition of Src activity with PP2 and shRNA targeted to Src abrogated chondrocyte proliferation and matrix synthesis (P < 0.05) and attenuated PLCγ1, MEK1/2 and ERK1/2 activation in chondrocytes subjected to periodic mechanical stress (P < 0.05). These findings suggest that periodic mechanical stress promotes chondrocyte proliferation and matrix synthesis in part through the Src-PLCγ1-MEK1/2-ERK1/2 signaling pathway, which links these three important signaling molecules into a mitogenic cascade.


Subject(s)
Animals , Rats , Chondrocytes/cytology , Chondrocytes/enzymology , MAP Kinase Signaling System/physiology , Mitogen-Activated Protein Kinase 1/metabolism , Mitogen-Activated Protein Kinase Kinases/metabolism , Stress, Mechanical , MAP Kinase Signaling System/genetics , Mitogen-Activated Protein Kinase 1/genetics , Mitogen-Activated Protein Kinase Kinases/genetics , Mitogens/metabolism , Phospholipase C gamma/metabolism , Rats, Sprague-Dawley , src-Family Kinases/metabolism
17.
Biol. Res ; 44(4): 369-375, 2011. ilus, tab
Article in English | LILACS | ID: lil-626737

ABSTRACT

Adiponectin is a polypeptide specifically secreted from human adipocytes, and its deficiency is closely linked to increased obesity and type II diabetes. There is an urgent demand for large-scale production of human adiponectin for pharmaceutical applications. Here, we report that we have successfully obtained a high-level of expression of modified genes encoding human adiponectin in transgenic rice. The 735 bp cDNA of the native human sequence was adopted to rice codon usage, fused to the translation initiation sequence in the N terminus and to the KDEL signal sequence in the C terminus. An amplification promoting sequence acting as an enhancer of transcription was also introduced to enhance gene expression. The presence of the transgene and mRNA transcripts was confirmed by PCR, Southern blot and RT-PCR. Western blot analysis revealed that a protein of approximately 30 kDa was produced in rice leaves. ELISA analysis was used to determine the amount of recombinant adiponectin in transformants with the modified gene in up to 0.32% of total soluble leaf protein. Our results establish the feasibility of high-level expression of recombinant human adiponectin in transgenic rice.


Subject(s)
Adiponectin/genetics , Oryza/genetics , Plants, Genetically Modified/genetics , Adiponectin/metabolism , Blotting, Southern , Codon , DNA, Complementary , Gene Expression Regulation, Plant , Oryza/chemistry , Reverse Transcriptase Polymerase Chain Reaction
18.
J Biosci ; 2008 Mar; 33(1): 55-62
Article in English | IMSEAR | ID: sea-111221

ABSTRACT

According to the method used in our laboratory,our group synthesized (DIPP-Trp)2-Lys-OCH 3. It inhibited the proliferation of K562 and HeLa cells in a dose-and time-dependent manner with an IC 50 of 15.12 and 42.23 microM, respectively. (DIPP-Trp) 2-Lys-OCH3 induced a dose-dependent increase of the G2/M cell population in K562 cells, and S cell population in HeLa cells;the sub-G0 population increased dramatically in both cell lines as seen by PI staining experiments using a FACS Calibur Flow cytometer (BeckmanCoulter,USA). Phosphatidylserine could signi?cantly translocate to the surface of the membrane in (DIPP-Trp)2-Lys-OCH3-treated K562 and HeLa cells.The increase of an early apoptotic population was observed in a dose-dependent manner by both annexin-FITC and PI staining.It was concluded that (DIPP-Trp) 2-Lys-OCH3 not only induced cells to enter into apoptosis,but also affected the progress of the cell cycle.It may have arrested the K562 and HeLa cells in the G 2/M,S phases,respectively.The apoptotic pathway was pulsed at this point,resulting in the treated cells entering into programmed cell death.(DIPP- Trp)-Lys-OCH is a potential anticancer drug that intervenes in the signalling pathway.


Subject(s)
Annexins/metabolism , Apoptosis/drug effects , Cell Proliferation/drug effects , Dose-Response Relationship, Drug , Flow Cytometry , Fluorescein-5-isothiocyanate/metabolism , Fluorescent Dyes/metabolism , G2 Phase/drug effects , HeLa Cells , Humans , Inhibitory Concentration 50 , K562 Cells , Mitosis/drug effects , Molecular Structure , Oligopeptides/chemical synthesis , Phosphopeptides/chemical synthesis , S Phase/drug effects , Tetrazolium Salts/analysis , Thiazoles/analysis , Time Factors
SELECTION OF CITATIONS
SEARCH DETAIL