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1.
Rev. bras. med. esporte ; 29: e2022_0261, 2023. tab, graf
Article in English | LILACS-Express | LILACS | ID: biblio-1387951

ABSTRACT

ABSTRACT Introduction College students represent a large proportion of amateur athletes in China. In sports practices, some unavoidable situations lead to physical injuries that can seriously affect the daily life of these students. Formulating rehabilitation plans to avoid secondary complications and speed up the students' return to their daily activities is necessary. Objective To study the causes of sports injuries and the effect of sports rehabilitation on university physical training. Methods Through questionnaires and interviews, 720 students and 25 teachers with a history of sports injuries were selected to investigate the causes of students' sports injuries. Then, 20 volunteers were selected as research subjects. They received a rehabilitation training protocol three times a week for six weeks. EMG signals of the athletes' shoulder and neck muscles were measured. Results Sports injury is an inevitable problem during college physical training. Training joint flexibility and stability can effectively improve the surface EMG signals in the area adjacent to the joint to improve muscle strength and joint amplitude level. Conclusion Students should consciously undertake rehabilitation training under professional guidance and receive full instruction in sports rehabilitation procedures involving a combination of physical and psychological recovery. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.


RESUMO Introdução Estudantes universitários representam grande parte dos esportistas amadores na China. Nas práticas esportivas, algumas situações inevitáveis acarretam em lesões físicas que podem afetar seriamente o cotidiano desses alunos. Faz-se necessária a formulação de planos de reabilitação afim de evitar complicações secundárias e agilizar o retorno dos estudantes às suas atividades diárias. Objetivo Estudar as causas de lesões esportivas e o efeito da reabilitação esportiva no treinamento físico universitário. Métodos 720 alunos e 25 professores com histórico de lesão esportiva foram selecionados para investigar as causas de lesão esportiva dos alunos por meio de questionário e entrevista. Em seguida, foram selecionados 20 voluntários como objeto de pesquisa. Esses receberam um protocolo de treinamento em reabilitação três vezes por semana, durante 6 semanas. Foram mensurados os sinais EMG dos músculos do ombro e pescoço dos atletas. Resultados A lesão esportiva é um problema inevitável durante o treinamento físico universitário. Treinar a flexibilidade e estabilidade articular pode efetivamente melhorar os sinais EMG de superfície na área adjacente à articulação, de modo a melhorar a força muscular e o nível de amplitude articular. Conclusão Os alunos devem realizar conscientemente treinamento de reabilitação sob orientação profissional, além de receberem instrução plena sobre os procedimentos da reabilitação esportiva, envolvendo uma combinação de recuperação física e psicológica. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.


RESUMEN Introducción Los estudiantes universitarios representan una gran parte de los deportistas aficionados en China. En las prácticas deportivas, algunas situaciones inevitables provocan lesiones físicas que pueden afectar gravemente a la vida cotidiana de estos alumnos. Es necesario formular planes de rehabilitación para evitar complicaciones secundarias y acelerar el regreso de los alumnos a sus actividades cotidianas. Objetivo Estudiar las causas de las lesiones deportivas y el efecto de la rehabilitación deportiva en el entrenamiento físico universitario. Métodos Se seleccionaron 720 estudiantes y 25 profesores con antecedentes de lesiones deportivas para investigar las causas de las lesiones deportivas de los estudiantes mediante un cuestionario y una entrevista. A continuación, se seleccionaron 20 voluntarios como sujetos de la investigación. Recibieron un protocolo de entrenamiento de rehabilitación tres veces por semana, durante 6 semanas. Se midieron las señales EMG de los músculos del hombro y del cuello de los atletas. Resultados Las lesiones deportivas son un problema inevitable durante el entrenamiento físico universitario. El entrenamiento de la flexibilidad y la estabilidad articulares pueden mejorar eficazmente las señales EMG superficiales en la zona adyacente a la articulación, de modo que se mejore la fuerza muscular y el nivel de amplitud articular. Conclusión Los estudiantes deben realizar conscientemente un entrenamiento de rehabilitación bajo la dirección de un profesional, y recibir una instrucción completa en los procedimientos de rehabilitación deportiva que impliquen una combinación de recuperación física y psicológica. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

2.
Rev. Assoc. Med. Bras. (1992) ; 68(2): 170-175, Feb. 2022. tab, graf
Article in English | LILACS | ID: biblio-1365349

ABSTRACT

SUMMARY OBJECTIVE: A growing volume of literature has suggested long noncoding RNAs (lncRNAs) as important players in tumor progression. In this study, we aimed to investigate the expression and prognostic value of lncRNA LINC00173 (LINC00173) in melanoma. METHODS: LINC00173 expression was measured in 163 paired cancerous and noncancerous specimen samples by real-time polymerase chain reaction. The correlations between LINC00173 expression with clinicopathological characteristics and prognosis were analyzed by chi-square test, log-rank test, and multivariate survival analysis. Receiver-operating characteristic curves were used for the assessment of the diagnostic value of LINC00173 for melanoma patients. RESULTS: The expression level of LINC00173 in melanoma specimens was distinctly higher than that in adjacent non-neoplasm specimens (p<0.01). Besides, LINC00173 was expressed more frequently in patients with advanced melanoma than in patients with early melanoma. Multivariate assays confirmed that LINC00173 expression level was an independent prognostic predictor of melanoma patients (p<0.05). CONCLUSION: Our data indicated that LINC00173 expression could serve as an unfavorable prognostic biomarker for melanoma patients.


Subject(s)
Humans , RNA, Long Noncoding/genetics , RNA, Long Noncoding/metabolism , Melanoma/diagnosis , Melanoma/genetics , Prognosis , Biomarkers, Tumor/genetics , Gene Expression Regulation, Neoplastic , Kaplan-Meier Estimate
3.
Braz. j. infect. dis ; 26(1): 101701, 2022. graf
Article in English | LILACS-Express | LILACS | ID: biblio-1364542

ABSTRACT

Abstract Severe lactic acidosis, a mitochondrial toxicity caused by the recommended standard dosage of linezolid (LZD), may occur in patients with impaired renal function. We describe an adult male who underwent kidney transplantation with stably impaired renal function, severe dyspnea, and abdominal discomfort. He received a standard oral dose of LZD (600 mg twice daily) and azithromycin for three weeks with a reduced immunosuppressant dose due to pulmonary non-tuberculosis mycobacterial infection. He was alert and afebrile, with a blood pressure of 140/60 mmHg. Pertinent laboratory data showed: pH 7.12, PaCO2 13.6 mmHg; HCO3- 4.3 mmol/L and serum lactate 18.4 mmol/L. His trough serum LZD concentration reached toxic levels (21.4 μg/mL). With hemodialysis, his clinical symptoms improved, with a decline in serum LZD (9.8μg/mL) and lactate (3.2 mmol/L). Chronic standard dose LZD in patients with impaired renal function can lead to life-threatening lactic acidosis, especially in coexisting conditions that reduce LZD metabolism.

4.
Arch. Clin. Psychiatry (Impr.) ; 48(5): 216-220, Sept.-Oct. 2021. tab
Article in English | LILACS-Express | LILACS | ID: biblio-1364288

ABSTRACT

ABSTRACT Recent research has shown that genetic variations in the oxytocin receptor (OXTR) may be related to variations in subtypes of obsessive-compulsive disorder (OCD). We aimed to explore the relationship between different subtypes of OCD and the genetic variation between rs1316193 and rs4686301 of the OXTR. In this case-control study, 92 OCD patients and 92 healthy controls were included in the OCD and control groups, respectively. The Y-BOCS scale was used to assess the severity of the OCD symptoms. The fasting peripheral blood samples were collected to extract DNA. rs4686301 and rs13316193 were genotyped using restriction fragment length polymorphism analysis techniques. Whether the gene frequency of the locus and the distribution of allele frequency were related to OCD were further study by TaqMan allele typing. The rs4686301 locus differed significantly between behavior and control groups. The genotype frequency and allele frequency at the rs4686301 locus were statistically significant between behavior and control groups (P<0.05). There was significant difference in the genotype frequency at the rs13316193 locus between behavior and control groups (P<0.05). The rs4686301 polymorphism of the OXTR may affect the clinical subtype of OCD. The rs13316193 polymorphism of the OXTR may be a risk factor for obsessive-compulsive behavior.

5.
Rev. bras. med. esporte ; 27(spe): 83-86, Mar. 2021. tab, graf
Article in English | LILACS | ID: biblio-1156145

ABSTRACT

ABSTRACT It is particularly important to improve the quality of physical education curriculum and the overall health level of college students. It is difficult to achieve a comprehensive evaluation of the original evaluation method of students' physical health level. Therefore, this study combined the analytic hierarchy process (AHP) and physical fitness training mode to build a university physical health evaluation model. Taking X University as the experimental background, 20 students of different majors in grade 2019 were selected as experimental objects. One group (10 students) adopted the original physical education curriculum mode, and the other group (10 students) integrated physical fitness training into the physical education curriculum. Using the established health evaluation model, the evaluation scores of physical health level of two groups of college students before and after the experiment were comprehensively analyzed. The results showed that the overall score of group A students' physical health level increased; the rising rate was 2.87%. However, there was no significant change in the scores of physical health assessment of group B students. The results show that physical fitness training into the public physical education curriculum can effectively help improve the overall health level of students, and also shows that the introduction of AHP into the evaluation of College Students' physical health level can improve the comprehensiveness of health level analysis. It is hoped that this study can provide some reference for physical education teaching in Colleges and universities, so as to improve the overall teaching level of colleges and universities.


RESUMO É particularmente importante melhorar a qualidade dos programas de educação física e o nível geral de saúde dos estudantes universitários. É difícil obter uma avaliação abrangente do método de avaliação inicial do nível de saúde física dos estudantes. Por conseguinte, este estudo combinou o processo hierárquico analítico (AHP) e o modo de condicionamento físico para construir um modelo universitário de avaliação da saúde física. Tendo a Universidade X como objeto experimental, vinte estudantes de diferentes especializações do período de 2019 foram selecionados como objetos experimentais. Um grupo ( de dez estudantes) adotou o modo de educação física original, e o outro grupo (de dez estudantes) integrou o condicionamento físico no currículo de educação física. Utilizando o modelo estabelecido de avaliação da saúde, as pontuações de avaliação do nível de saúde física de dois grupos de estudantes universitários antes e depois do experimento foram exaustivamente analisadas. Os resultados mostraram que a pontuação global do nível de saúde física de um grupo de estudantes aumentou. A taxa de aumento foi de 2.87%. No entanto, não houve alterações significativas nas pontuações da avaliação para a saúde física dos estudantes do grupo B. Os resultados mostram que o condicionamento físico no currículo público de educação física pode contribuir efetivamente para melhorar o nível geral de saúde dos estudantes, e também mostra que a introdução do processo hierárquico analítico (AHP) na avaliação do nível de saúde física dos estudantes universitários pode melhorar a abrangência da análise do nível de saúde. Espera-se que este estudo possa fornecer uma referência para o ensino de educação física nos colégios e universidades a fim de melhorar o nível de ensino geral das universidades.


RESUMEN Es particularmente importante mejorar la calidad del plan de estudios de educación física y el nivel de salud general de los estudiantes universitarios. Es difícil lograr un análisis integral del método de evaluación original del nivel de salud física de los estudiantes. Por lo tanto, este estudio combinó el proceso de jerarquía analítica (PJA) y el modo de entrenamiento de aptitud física para construir un modelo universitario de evaluación de la salud física. Tomando la Universidad X como base experimental, se seleccionaron como sujetos experimentales 20 estudiantes de diferentes carreras en los cursos de 2019. Un grupo (10 estudiantes) adoptó el modo de plan de estudios de educación física original y el otro grupo (10 estudiantes) integró el entrenamiento de aptitud física en el plan de estudios de educación física. Utilizando el modelo de evaluación de salud establecido, se analizaron exhaustivamente los puntajes de evaluación del nivel de salud física de los dos grupos de estudiantes universitarios antes y después del experimento. Los resultados mostraron que el puntaje general del nivel de salud física de los estudiantes del grupo A aumentó; la tasa de incremento fue del 2,87%. Sin embargo, no hubo cambios significativos en las puntuaciones de la evaluación de la salud física de los estudiantes del grupo B. Los resultados muestran que el entrenamiento de la aptitud física en el plan de estudios público de educación física puede ayudar de manera efectiva a mejorar el nivel de salud general de los estudiantes y también muestra que la introducción de PJA en la evaluación del nivel de salud física de los estudiantes universitarios puede mejorar la precisión del análisis del nivel de salud. Esperamos que este estudio pueda proporcionar una referencia para la enseñanza de la educación física en los colegios y universidades, a fin de mejorar su nivel general en estos establecimientos.


Subject(s)
Humans , Physical Education and Training , Sports/education , Universities , Health Status , Curriculum , Physical Conditioning, Human
6.
Chinese Journal of School Health ; (12): 1720-1723, 2021.
Article in Chinese | WPRIM | ID: wpr-906576

ABSTRACT

Objective@#To analyze the impact of life satisfaction and parental rearing methods on accidental injuries of primary and secondary school students in rural areas, and to provide a therotical reference for injury prevention and intervention among primary and secondary school students in rural areas.@*Methods@#A stratified cluster random sampling method was used to select 2 045 rural primary and middle school students in Luliang County, Yunnan Province, questionnaire survey regarding injury, MSLSS and EMBU was administered.@*Results@#The incidence of accidental injury among rural primary and middle school students was 16.19 %, and the rate of boys (19.51%) were higher than that of girls (12.26%) ( χ 2=19.69, P <0.01). Multivariate Logistic regression analysis found that women, low injury risk behavior scores, and high life satisfaction scores were associated with less accidental injuries( OR =0.62, 0.98,0.81), while day to day school and father s overprotection were associated with more for accidental injuries( OR =1.50, 1.04, P <0.05).@*Conclusion@#Parents should pay more attention to children s safety education while creating a good living environment for their children, but they should pay attention to their own education methods to reduce accidental injuries.

7.
Article in Chinese | WPRIM | ID: wpr-887413

ABSTRACT

@#[摘 要] 目的:探讨大荨麻提取物对乳腺癌细胞增殖、凋亡和细胞周期的影响,并初步探讨其可能的作用机制。方法:用不同质量浓度的大荨麻提取物(0、1、2、4、8、16、32、64 mg/ml)处理乳腺癌细胞MCF-7和MDA-MB-231 24 h,MTT法检测细胞增殖活力,选择中位抑制浓度附近的浓度(5和10 mg/ml)作为给药浓度分别处理MCF-7和MDA-MB-231细胞24 h后,平板克隆形成实验和流式细胞术分别检测大荨麻提取物对乳腺癌细胞增殖、周期和凋亡的影响,WB法检测对细胞周期和凋亡相关蛋白以及PI3K/AKT信号通路相关蛋白表达的影响。在MCF-7细胞用5 mg/ml大荨麻提取物处理的同时转染过表达AKT质粒(大荨麻+AKT组),转染空载质粒为对照组(大荨麻+vec组),WB法检测过表达效率,比较过表达AKT对细胞增殖、周期和凋亡的影响。结果:各大荨麻提取物处理组MCF-7和MDA-MB-231细胞增殖活力均显著低于对照组(P<0.05或P<0.01);与对照组比较,5或10 mg/ml大荨麻处理组乳腺癌细胞的克隆形成数显著减少,G0/G1期细胞占比和凋亡率显著增加(P<0.05或P<0.01),P21、BAX蛋白表达显著升高而Cyclin D1、CDK4、Bcl2蛋白以及p-PI3K、p-AKT蛋白表达显著降低(P<0.05或P<0.01)。大荨麻+AKT组p-AKT和AKT蛋白表达显著高于大荨麻+vec组,克隆数、S期和G2/M期细胞占比均高于大荨麻+vec组(P<0.05或P<0.01),G0/G1期细胞占比和凋亡率低于大荨麻+vec组(P<0.05或P<0.01)。结论:大荨麻提取物可以抑制乳腺癌细胞增殖、促进凋亡且阻滞细胞在G0/G1期,其作用机制可能与抑制PI3K/AKT信号通路相关。

8.
Braz. j. med. biol. res ; 53(11): e9551, 2020. graf
Article in English | LILACS, ColecionaSUS | ID: biblio-1132485

ABSTRACT

The objective of this study was to investigate the effect of Mycobacterium vaccae on Jagged 1 and gamma delta T17 (γδT17) cells in asthmatic mice. An asthma mouse model was established through immunization with ovalbumin (OVA). Gamma-secretase inhibitor (DAPT) was used to block the Notch signaling pathway. M. vaccae was used to treat asthma, and related indicators were measured. Blocking Notch signaling inhibited the production of γδT17 cells and secretion of cytokine interleukin (IL)-17, which was accompanied by a decrease in Jagged1 mRNA and protein expression in the treated asthma group compared with the untreated asthma group. Similarly, treatment with M. vaccae inhibited Jagged1 expression and γδT17 cell production, which was associated with decreased airway inflammation and reactivity. The Notch signaling pathway may play a role in the pathogenesis of asthma through the induction of Jagged1 receptor. On the other hand, the inhibitory effect of M. vaccae on Jagged1 receptor in γδT17 cells could be used for the prevention and treatment of asthma.


Subject(s)
Animals , Rabbits , Signal Transduction , Mycobacterium , Ovalbumin , Receptors, Notch , Jagged-1 Protein
9.
Chinese Journal of School Health ; (12): 228-231, 2020.
Article in Chinese | WPRIM | ID: wpr-809757

ABSTRACT

Objective@#To explore the associations of aggressive behavior with self-esteem with suicide risk in college students.@*Methods@#A total of 4 154 college students in Suzhou, Hefei and Ma’anshan were enrolled in our study by multistage cluster sampling method during October to December 2017. Demographic characteristics, suicide risk, aggression and self-esteem of participants were evaluated by Suicidal Behaviors Questionnaire-Revised (SBQ-R), Buss & Perry Aggression Questionnaire-Chinese Version (AQ-CV) and Rosenberg Self-esteem Questionnaire separately. Aggression and self-esteem were divided into three levels, high (> P 75 ), medium ( P 25 - P 75 ) and low (< P 25 ) level, according to the percentile of the questionnaire score. Multivariate logistic regression model was used to examine the associations of aggression and self-esteem with suicide risk, and to explore gender difference.@*Results@#The gender differences were found in suicide risk, aggressive behavior and self-esteem( χ 2=80.21, 26.47, 26.51 , P <0.01). More girls reported suicide ideation, suicide plan and suicide attempt compared to girls( χ 2=33.03, 25.11, 15.71 , P <0.01). Compared with boys, the detection rate of suicide ideation, suicide plan and suicide attempt of girls were higher( P <0.01). The high aggressive behavior and low self-esteem was correlated with increased risk of suicide, and the interaction effects of aggressive behavior and self-esteem on suicide risk was found. The students with high aggressive behavior and low self-esteem had a higher suicide risk, the multiple adjusted odds ratio was 19.39 (95% CI =11.10-33.87). Gender difference was not found in the associations of aggressive behavior and self-esteem with suicide risk ( P >0.05).@*Conclusion@#Aggressive behavior and self-esteem are correlated with suicide risk in college students.

10.
Chinese Journal of School Health ; (12): 1346-1349, 2020.
Article in Chinese | WPRIM | ID: wpr-829124

ABSTRACT

Objective@#To explore the relationship between parental control and aggressive behavior of middle school students.@*Methods@#Random cluster sampling was used to select 1 046 students.Information was collected through the Chinese version of Parental Control Questionnaire and Buss & Perry Aggression Questionnaire.@*Results@#The score on anger were high among female students and the total score were high among male students(t=6.28,2.56,P<0.01).Multiple comparison showed. the grade two junior middle school students’aggression level is significantly higher than grade three junior middle school students and grade one, two high school students(t=3.22,4.07,3.01,P<0.05). There were significant gender differences in parental control,father’s behavior control and father’s psychological control(P<0.05).Parental control shows a significant grade difference,multiple comparison showed, parental control among junior middle school students is significantly higher than that among high school students(t=3.22, 4.07, 3.01, P<0.05).Parental control and aggressiveness of the students were significantly positively correlated(r=0.16,0.29).There was a significant positive correlation between the psychological control of the parents and the aggressiveness of the boys(r=0.26,0.23).The behavior control and psychological control of the parents were positively correlated with the aggressiveness of the girls(r=0.09,0.13,0.29,0.35)(P<0.05).The Logistic regression analysis showed,father’s psychological control(OR=0.50,95%CI=0.21-0.79) and mother’s psychological control(OR=0.32,95%CI=0.02-0.62) showed significant positive association with aggression of the boys.Mother’s psychological control(OR=0.55,95%CI=0.23-0.87)and parents’ control(OR=0.17,95%CI=0.01-0.33) were positively associated with aggression of the girls(P<0.05).@*Conclusion@#Effective intervention of parental control might help prevent and control aggressive behavior of middle school students.

11.
J Genet ; 2019 Nov; 98: 1-12
Article | IMSEAR | ID: sea-215392

ABSTRACT

Studies have shown that many complex diseases are sex-determined. When conducting genetic association studies on Xchromosome, there are two important epigenetic factors which should be considered simultaneously: X-chromosome inactivation and genomic imprinting. Currently, there have been several association tests accounting for the information on X-chromosome inactivation. However, these tests do not take the imprinting effects into account. In this paper, we propose a novel association test simultaneously incorporating X-chromosome inactivation and imprinting effects based on case–parent trios and control–parent trios for female offspring and case–control data for male offspring, denoted by MLRXCII. Extensive simulation studies are carried out to investigate the type I error rate and the test power of the proposed MLRXCII . Simulation results demonstrate that the proposed test controls the type I error rate well andis more powerful than the existing method when imprinting effects exist. The proposed MLRXCII test is valid and powerful in genetic association studies on X-chromosome for qualitative traits and thus is recommended in practice.

12.
Electron. j. biotechnol ; 34: 37-42, july. 2018. tab, graf
Article in English | LILACS | ID: biblio-1045997

ABSTRACT

Background: Astaxanthin from natural sources is typically esterified with fatty acids; hence, it must be hydrolyzed to remove esters before identification and quantification by conventional HPLC. Alkaline-catalyzed saponification and enzyme-catalyzed enzymolysis are the most commonly used de-esterification methods. However, information on the efficiency and isomerization during de-esterification of natural astaxanthin esters by these two methods remains scarce. Therefore, we conducted two HPLC-based experiments to determine which method is better for hydrolyzing astaxanthin esters. Results: To assess the effect of enzymolysis (0.67 U/mL cholesterol esterase, at 37°C) and saponification (0.021 M NaOH, at 5°C) conditions on free astaxanthin recovery and destruction or structural transformation of astaxanthin, we varied the total treatment time across a range of 195 min. The results showed that enzymolysis and saponification were complete in 60 min and 90 min, respectively. After complete hydrolysis, the maximum free astaxanthin recovery obtained by enzymolysis was 42.6% more than that obtained by saponification. The identification of by-products, semi-astacene and astacene, during the process of saponification also indicated that a more severe degradation of astaxanthin occurred during saponification. Moreover, the composition of astaxanthin isomers during saponification was similar to that of the isomers during enzymolysis between 30 min and 75 min (all-trans:9-cis:13-cis = 21:3:1, approximately) but dramatically changed after 90 min, whereas the composition in the enzymolysis treatment remained relatively stable throughout. Conclusion: Compared with saponification, enzymolysis with cholesterol esterase was recommended as a more accurate method for de-esterification of natural astaxanthin esters for further qualitative and quantitative HPLC analysis.


Subject(s)
Xanthophylls/chemistry , Esters/chemistry , Carotenoids , Xanthophylls/metabolism , Alkalies , Enzymes/metabolism , Esters/metabolism , Hydrolysis , Isomerism
13.
Article in Chinese | WPRIM | ID: wpr-749776

ABSTRACT

@#Objective     To evaluate efficacy of amiodarone in the prevention of atrial fibrillation after coronary artery bypass grafting. Methods     CBM (from January 1978 to August 2017), CNKI (from January 1987 to August 2017), VIP (from January 1989 to August 2017), Wanfang (from January 1998 to August 2017) and PubMed (from January 1989 to August 2017) databases were searched. The articles were selected based on the inclusion and exclusion criteria. Quality of articles was assessed by improved Jadad scale. Statistical analysis was performed using RevMan 5.3. Results     There were 19 articles meeting inclusion criteria including 2 817 patients and all were randomized controlled trial (RCT). There were 16 articles with high quality and 3 articles with low quality by improved Jadad scale. Compared with the placebo, amiodarone had a significant effect on reducing the incidence of atrial fibrillation after coronary artery bypass grafting (RR=0.37, 95% CI 0.28 to 0.50, P<0.000 01) and different administration models and time of amiodarone had effect on the atrial fibrillation after aterial bypass grafting (P<0.05). Conclusion    Compared with the placebo, amiodarone is effective in reducing the incidence of atrial fibrillation after coronary artery bypass grafting.

14.
Braz. j. microbiol ; 49(supl.1): 93-100, 2018. tab, graf
Article in English | LILACS | ID: biblio-974332

ABSTRACT

Abstract Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and Ser87-Ile in parC, was detected in 33 K. pneumoniae isolates. All the isolates harbored at least two virulence factor genes, with ureA (97.0%) and wabG (91.0%) exhibiting high carriage rates in 33 K. pneumoniae isolates. MLST revealed 7 sequence types, of which 3 STs (2541, 2581 and 2844) were newly assigned. Using eBURST, ST2844 and ST2541 were assigned to new clonal complex 2844. Our study provides evidence and biological characteristics of K. pneumoniae isolates from cattle upper respiratory tract in Southwest China.


Subject(s)
Animals , Cattle , Bacterial Proteins/genetics , Klebsiella Infections/veterinary , Cattle Diseases/microbiology , Drug Resistance, Multiple, Bacterial , Virulence Factors/genetics , Klebsiella pneumoniae/isolation & purification , Klebsiella pneumoniae/drug effects , Anti-Bacterial Agents/pharmacology , Bacterial Proteins/metabolism , Klebsiella Infections/microbiology , China , Virulence Factors/metabolism , Multilocus Sequence Typing , Klebsiella pneumoniae/genetics , Klebsiella pneumoniae/metabolism
15.
An. bras. dermatol ; 92(3): 350-355, May-June 2017. tab, graf
Article in English | LILACS | ID: biblio-886948

ABSTRACT

Abstract Background: Photodynamic therapy with topical aminolevulinic acid (ALA-PDT) has been suggested to be effective in treatment of acne vulgaris. However, adverse events occur during and after treatment. Objectives: To compare the efficacy and tolerability of optical intra-tissue fiber irradiation (OFI) ALA-PDT versus traditional ALA-PDT in treatment of acne vulgaris in rabbit models. Methods: Twenty-five rabbits of clean grade were used. Twenty rabbits were randomly selected to establish acne model and the other five were used as control. Rabbits in model group (40 ears) were further divided into four groups (10 ears/group): I, OFI-ALA-PDT with the head of optical fiber inserted into the target lesion (intra-tissue); II, traditional ALA-PDT group; III, OFI group; IV, blank control group without any treatment. Uncomfortable symptoms, adverse events, and effectiveness rates were recorded on post-treatment day 14, 30, and 45. Results: On post-treatment day 14, the effectiveness rate in OFI-ALA-PDT group was obviously higher than that of the other three groups (P<0.05). However, no improved effects were observed in OFI-ALA-PDT group on day 30 and 45. During the period of treatment, the frequencies of uncomfortable symptoms in ALA-PDT group were obviously higher than those in the other three groups (P<0.05). The adverse event rate in OFI-ALA-PDT group was obviously lower than that of the ALA-PDT group (P<0.05) Study limitations: The unblindness of the study and temporary animal models of acne induced may hamper the assessment and monitoring of the results, and future studies are still needed to clarify it further. Conclusion: The OFI-ALA-PDT group (intra-tissue irradiation) showed no improved efficacy on treating rabbit ear acne but had higher safety and better tolerability.


Subject(s)
Animals , Rabbits , Photochemotherapy/methods , Photosensitizing Agents/administration & dosage , Acne Vulgaris/therapy , Aminolevulinic Acid/administration & dosage , Photochemotherapy/adverse effects , Random Allocation , Treatment Outcome , Photosensitizing Agents/adverse effects , Disease Models, Animal , Aminolevulinic Acid/adverse effects
16.
An. bras. dermatol ; 92(3): 319-322, May-June 2017. tab
Article in English | LILACS | ID: biblio-886975

ABSTRACT

Abstract Background: Von Zumbusch type of generalized pustular psoriasis is a rare variant of psoriasis in children. It can occur in patients with or without psoriasis vulgaris. Objective: The aim of the study was to discuss the precipitating factors, clinical manifestations, laboratory data and therapy of von Zumbusch type of generalized pustular psoriasis in children from southwestern China and to improve the diagnosis and treatment level. Methods: A retrospective analysis was conducted for inpatients aged 14 years old or less with von Zumbusch type of generalized pustular psoriasis in our department from 2005 to 2014. Results: A total of 26 patients were included, of whom four (15.38%) had previous history of psoriasis vulgaris and one (3.85%) had previous history of psoriasis arthropathica. Mean onset age was 6.90 years. Gender distribution was equivalent. Incidence of the disease in summer and autumn was higher than that in winter and spring. Nineteen (73.08%) cases were triggered by infection, two (7.69%) cases were caused by sudden discontinuation of systemic use of corticosteroid. Twenty-four (92.31%) cases had concomitant fever. The initial lesion manifested as non-follicular sterile pustules on erythema. Sixteen patients responded well to acitretin, 11 to Tripterygium wilfordii Hook F (TwHF), two to cyclosporine, and one to methotrexate. Study limitations: This study is a retrospective one and the number of cases is small. CONCLUSION: Von Zumbusch type of generalized pustular psoriasis is a rare disease in children, infection is the most common precipitating factor, acitretin is the first-line therapy, traditional Chinese medicine TwHF also can be used.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Psoriasis/diagnosis , Psoriasis/drug therapy , Psoriasis/classification , Psoriasis/etiology , China , Methotrexate/therapeutic use , Retrospective Studies , Cyclosporine/therapeutic use , Acitretin/therapeutic use
17.
Article in Chinese | WPRIM | ID: wpr-333484

ABSTRACT

Studies showed that the use of cyclic adenosine monophosphate (cAMP) substitutes or intracellular cAMP activators increased intracellular cAMP level,causing anti-inflammatory effects.This study was to investigate the effects of pretreatment with meglumine cyclic adenylate (MCA),a compound of meglumine and cAMP,on systemic inflammation induced by lipopolysaccharide (LPS) in rats.Eighteen adult male Sprague-Dawley rats were randomly divided into 3 groups (n=6 each):control group (NS group),LPS group (LPS group) and LPS with MCA pretreatment group (MCA group).Systemic inflammation was induced with LPS 10 mg/kg injected via the femoral vein in LPS and MCA groups.In MCA group,MCA 2 mg/kg was injected via the femoral vein 20 min before LPS injection,and the equal volume of normal saline was given in NS and LPS groups at the same time.Three hours after LPS injection,the blood samples were taken from the abdominal aorta for determination of plasma concentrations of TNF-α,IL-1,IL-6,IL-10,cAMP by ELISA and NF-r Bp65 expression by Western blotting.The experimental results showed that inflammatory and antiinflammatory indices were increased in LPS group compared to NS group;inflammatory indices were declined and anti-inflammatory indices were increased in MCA group relative to LPS group.Our study suggested that MCA pretreatment may attenuate LPS-induced systemic inflammation.

18.
Article in Chinese | WPRIM | ID: wpr-333434

ABSTRACT

This study attempts to discuss the correlation between UGT1A1*28 as uridine diphosphate glucuronosyltransferase gene promoter and coding region Gly71Arg gene polymorphism with neonatal hyperbilirubinemia of neonates in Wuhan.A total of 168 neonates were divided into the hyperbilirubinemia group (case group,n=108) and healthy neonates group (control group,n=60).Their DNA was obtained through blood extraction.The gene exon mutation of UGT1A1 was detected by Sanger sequencing,which revealed the relationship between UGT 1A 1*28 and Gly71Arg polymorphism with neonatal hyperbilirubinemia of neonates.The results showed that:(1) The frequency of UGT1Al*28 allele mutation in the case group and the control group was 9.3% and 10% respectively,with the difference being not significant between the two groups (P>0.05).(2) The frequency of Gly71Arg allele mutation in the case group and the control group was 35.1% and 21.7% respectively,with the difference being significant between the two groups (P<0.01).(3) The serum bilirubin level of Gly71Arg mutant homozygous and heterozygous subgroups (n=66) in the case group was 302.7±31.4 μmol/L,which was significantly higher than 267.3±28.5 μmol/L of the wild subgroup (n=42) (P<0.01).It was suggested that the occurrence of neonatal hyperbilirubinemia of neonates in Wuhan was not associated with UGT 1A1*28 gene polymorphism,but closely with the Gly71Arg gene polymorphism.Meanwhile,the Arg allele mutation was related to the degree of jaundice.

19.
Article in Chinese | WPRIM | ID: wpr-238384

ABSTRACT

Essential hypertension (EH) is affected by both genetic and environmental factors.The polymorphism of connexin (Cx) genes is found associated with the development of hypertension.However,the association of the polymorphism of Cxs with EH has not been investigated.This study aimed to investigate the association of the polymorphism of connexin (Cx) genes Cx37,Cx40,and Cx43 with EH in Kazak and Han Chinese in Xinjiang,China.Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were used to analyze the polymorphism of Cx genes in Kazak and Han EH patients as well as their normotensive controls.The results showed that there were no significant differences in the frequencies of different three genotypes (A/A,A/G,and G/G) and A and G alleles of Cx40 rs35594137 and rs11552588 between EH patients and normotensive controis.However,in Kazak EH patients,the frequencies of three genotypes (A/A,A/G;and G/G) of Cx37 rs1630310 were 24.8%,47.2% and 28.0%,respectively,which were significantly different from those in Han EH patients.In Han EH patients,the frequencies of the three genotypes (C/C,C/G and G/G) of Cx43 rs1925223 were 6.4%,35.6% and 58.0%,respectively.Frequencies of the other four genotypes had no statistical differences among Kazak and Hart EH patients and their normotensive controls.These results suggest polymorphisms of Cx37 rs 1630310 and Cx43 rs 1925223 genes may be associated with the pathogenesis of EH.Carrying Cx37 rs1630310-A or Cx43 rs1925223-G genotypes may protect against the development of EH.

20.
Article in Chinese | WPRIM | ID: wpr-822644

ABSTRACT

Objective @# To know the influencing factors of dental caries in first permanent molars among children aged 10-12 years old in Guangzhou, and to give references for dental caries prevention.@*Methods @#The samples were selected by stratified random sampling method. Oral health examination and questionnaires were applied to all the 18 656 children who were 5 or 6-grade in 149 primary schools. @*Results @#The prevalence of dental caries in first permanent molars was 18.19%. Girls accouted for 19.96%, which was significantly higher than that of boys (16.71%) (χ2 = 32.817, P< 0.001). Multiple regression analyses were used to investigate the influencing factors of dental caries in first permanent molars. After controlling school cluster and other factors, household register, gender, pit and fissure sealant, consumption of sweets and chocolates, consumption of sweet milk were influencing factors of permanent teeth caries. And household register, gender, pit and fissure sealant, consumption of sweets and chocolates were associated with mean DMFT.@*Conclusion @#school oral health education should focus on the consumption of sugars and cares should be put into the differences between urban and rural area. In addition, strengthening publicity of the sealant retention project,raising public awareness, and improving sealant retention quality are necessary to ensure the anti-caries effect.

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