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1.
Chinese Journal of School Health ; (12): 876-879, 2022.
Article in Chinese | WPRIM | ID: wpr-934827

ABSTRACT

Objective@#To explore the development and interactive correlations between boredom proneness, smoking and drinking behavior.@*Methods@#A total of 416 adolescents from one senior high school and one college in the Inner Monggol Autonomous Region were recruited to complete the short version boredom proneness scale, as well as smoking and drinking behavior scale at baseline and in the 12 month follow up.@*Results@#There were significant and positive correlation between boredom proneness and smoking and drinking behavior at both cross sectional levels (T1 r =0.30, 0.34, T2 r =0.24, 0.45, P <0.01). Significant autoregressive coefficients were observed for boredom proneness, smoking and drinking behavior in adolescents ( β=0.53, 0.61, 0.45, P < 0.01). Moreover, the cross lagged analyses revealed that the relationship between bordom proneness and smoking behavior was unilaterally influencing ( β=0.12, P<0.01; β=0.03, P >0.05), the relationship between bordom proneness and drinking behavior was bidirectional over the 12 months ( β=0.21, 0.09, P <0.05).@*Conclusion@#Boredom proneness of adolescents is closely related to smoking and drinking behavior, boredom proneness can positively predict smoking and drinking behavior, and drinking behavior can positively predict boredom proneness.

2.
Journal of Preventive Medicine ; (12): 457-461, 2021.
Article in Chinese | WPRIM | ID: wpr-877073

ABSTRACT

Objective@#To understand the behavioral characteristics of vaccination of human papillomavirus (HPV) vaccine based on diffusion of innovation theory, and to put forward suggestions for improving the vaccination willingness.@*Methods@#The women who had made an appointment or received at least one dose of HPV vaccine in two community health service centers with HPV vaccination qualification in Hangzhou were recruited by convenience sampling method. A questionnaire survey was conducted according to cervical cancer risk factors, HPV vaccine knowledge and the time of vaccination. The diffusion of HPV vaccination and the characteristics of vaccinees in different stages was analyzed.@*Results@#A total of 448 women, aged from 18 to 45 years old, with a median of 30.5 years old, were investigated. The spread of HPV vaccine were divided into five stages: April 2017 and before was the initial stage, with 8 leaders, accounting for 1.79%; October 2017 to February 2018 was the slowly rising stage, with 59 early vaccinees, accounting for 13.17%; April 2018 to April 2019 was the rising stage, with 160 most early vaccinees, accounting for 35.71%; May to August 2019 was the rapidly rising stage, with 170 most late vaccinees, accounting for 37.95%; September 2019 to the end of the survey was the standstill stage, with 51 laggards, accounting for 11.38%. All of the leaders had participated in cervical cancer screening, voluntarily vaccinated, and knew the susceptible population of cervical cancer, the targeted HPV types and the suitable population of vaccine. The median age of the first pregnancy was 27 years old. Six of them had three pregnancies. The early vaccinees and the most early vaccinees were recommended by doctors or friends to get HPV vaccine. The median age of first pregnancy was 26 and 28 years old, respectively. Their other characteristics were similar to those of the leaders. The characteristics of most late vaccinees were similar to those of the laggards. They were were mainly students who were recommended by their friends to receive HPV vaccination, did not participate in cervical cancer screening, did not know the susceptible population of cervical cancer, the targeted HPV types and the suitable population of vaccine.@*Conclusions@#People with high risk of HPV exposure, awareness of cervical cancer and screening are more likely to receive HPV vaccination at the early stage. It is necessary to strengthen the health education of HPV vaccination, and make full use of the influence of doctors and early vaccinees, which is helpful to promote the early vaccination of HPV vaccine among the right age population.

3.
Braz. j. med. biol. res ; 53(5): e9021, 2020. graf
Article in English | LILACS | ID: biblio-1098108

ABSTRACT

Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony formation. Wound healing and transwell invasion assays revealed that CHPF could positively regulate the migration of lung cancer cells. The tumorigenic role of CHPF was also validated in nude mouse xenograft models. Affymetrix gene chip analysis indicated that CHPF regulated the proliferation and invasion of lung cancer cells through CDH1, RRM2, MKI67, and TNFRSF10B. We thus highlight CHPF as a novel target for lung cancer treatment.


Subject(s)
Humans , Animals , Female , Rabbits , Gene Expression Regulation, Neoplastic , N-Acetylgalactosaminyltransferases/metabolism , Lung Neoplasms/metabolism , Blotting, Western , N-Acetylgalactosaminyltransferases/genetics , Cell Line, Tumor , Microarray Analysis , Cell Proliferation , Real-Time Polymerase Chain Reaction , Lung Neoplasms/genetics , Mice, Inbred BALB C
4.
J Environ Biol ; 2013 Apr; 34(2suppl): 459-464
Article in English | IMSEAR | ID: sea-148551

ABSTRACT

This study reports the performance of University of Cape Town (UCT) municipal wastewater treatment plant, during the startup stage with the focus on the relationship between hydraulic retention time (HRT) and biological nutrient removal (BNR) efficiency. The entire experimental period was 144 days, divided into four periods. Results showed that the removal efficiency of TN, NH4+-N, and Kjeldahl nitrogen (KN) was closely related to the HRT. Furthermore, the biodegradation kinetics analysis was used to calculate the specific degradation rates of pollutants. The GPS-X modeling was also used to examine the effect of the UCT pilot plant on BNR. The UCT pilot plant used in this study achieved high BNR efficiency even during the startup stage. With HRT of 24 hr (Period 1, day 1-40, data set 1-10), the highest levels of TN, NH4+-N and KN removal efficiency were approximately 72, 76 and 78%, respectively. The COD showed consistent high removal efficiency, with the highest level of approximately 96% at HRT of 15 hr (Period 3, day 81-120, data set 21-30). The TP removal efficiency rose at first and subsequently decreased abruptly. The maximum removal efficiency was 85% with HRT of 19 hr (Period 2, day 41-80, data set 11-20). With the optimal HRT 19 hr, the average removal efficiency values of COD, TP, TN, NH4+-N and KN were 89, 80, 65, 67 and 68%, respectively. The GPS-X modeling results indicated that the UCT process was effective in COD, TP and TN removal.

5.
Clinics ; 68(1): 19-25, Jan. 2013. tab
Article in English | LILACS | ID: lil-665913

ABSTRACT

OBJECTIVES: Hyperuricemia is a risk factor for contrast-induced acute kidney injury in patients with chronic kidney disease. This study evaluated the value of hyperuricemia for predicting the risk of contrast-induced acute kidney injury in patients with relatively normal serum creatinine who were undergoing percutaneous coronary interventions. METHODS AND RESULTS: A total of 788 patients with relatively normal baseline serum creatinine (<1.5 mg/dL) undergoing percutaneous coronary intervention were prospectively enrolled and divided into a hyperuricemic group (n = 211) and a normouricemic group (n = 577). Hyperuricemia is defined as a serum uric acid level>7 mg/ dL in males and >6 mg/dL in females. The incidence of contrast-induced acute kidney injury was significantly higher in the hyperuricemic group than in the normouricemic group (8.1% vs. 1.4%, p<0.001). In-hospital mortality and the need for renal replacement therapy were significantly higher in the hyperuricemic group. According to a multivariate analysis (adjusting for potential confounding factors) the odds ratio for contrast-induced acute kidney injury in the hyperuricemic group was 5.38 (95% confidence interval, 1.99-14.58; p = 0.001) compared with the normouricemic group. The other risk factors for contrast-induced acute kidney injury included age >75 years, emergent percutaneous coronary intervention, diuretic usage and the need for an intra-aortic balloon pump. CONCLUSION: Hyperuricemia was significantly associated with the risk of contrast-induced acute kidney injury in patients with relatively normal serum creatinine after percutaneous coronary interventions. This observation will help to generate hypotheses for further prospective trials examining the effect of uric acid-lowering therapies for preventing contrast-induced acute kidney injury.


Subject(s)
Aged , Female , Humans , Male , Middle Aged , Acute Kidney Injury/chemically induced , Contrast Media/adverse effects , Creatinine/blood , Hyperuricemia/complications , Percutaneous Coronary Intervention/adverse effects , Age Factors , Acute Kidney Injury/mortality , Coronary Angiography/adverse effects , Epidemiologic Methods , Hyperuricemia/mortality , Hyperuricemia/urine , Kidney/drug effects , Risk Factors , Sex Factors
6.
J Environ Biol ; 2013 Jan; 34(1): 79-85
Article in English | IMSEAR | ID: sea-148494

ABSTRACT

Nursery nitrogen application has been used to improve seedling quality. The technique has received little attention with bare-root seedlings and their subsequent field performance on weed competition sites. Our research objective was to examine responses of one- and two- year-old bare-root Olga Bay larch (Larix olgensis Henry) seedlings to nursery nitrogen supplements and subsequent one-year field performance on a competitive site. The fertilizer levels (kg N ha-1) were 0 (control), 60 (conventional fertilization, 60 C), 120 (additional nitrogen applied two times, 120 L), 180 (additional nitrogen applied three times, 180 L) and N were applied in increments of 30 kg ha-1 at 15-day interval to maintain a base nutrient level. Although pre-planting morphological attributes and nitrogen status of one-year-old (1a) seedlings were more sensitive to 60 C than for two-year-old (2a) seedlings, the conventional application failed to enhance their field survival(15.6% vs 17.8%), relative height growth (89.0% vs 79.6%), and relative diameter growth (17.0% vs 22.9%). The 1a seedlings‘ field survival (15.6% for 0, 17.8% for 60 C) and 2a seedlings‘ relative height growth rate (11.0% for 0, 8.9% for 60 C) were not increased significantly until they were provided the 120 L (survival of 23.3% for 1a, relative height growth rate of 15.0% for 2a). According to pre-planting attributes and field performance, optimum nursery nitrogen application was 120 L for the 2a seedlings and 180 L for 1a seedlings. Except for component nitrogen concentration, pre-planting morphological attributes and component N content for the 2a seedlings were as much 3.3 to 37.7 times that of 1a seedlings. In conclusion, the contrasting survival of poor (15.6%-28.9%) for 1a seedlings and high (84.4%-91.1%) for 2a seedlings indicated that additional nitrogen fertilizer would not equal the benefits of an another year‘s growth in the nursery. Successful reforestation could not be fulfilled by 1a seedlings regardless of their pre-nutrients. An alternative technique for sites with competing vegetation was to apply 120kg N ha-1 in the nursery during July and August on 2a seedlings.

7.
Clinics ; 68(2): 263-268, 2013. ilus, tab
Article in English | LILACS | ID: lil-668816

ABSTRACT

OBJECTIVE: Ischemia reperfusion injury is partly responsible for the high mortality associated with induced myocardial injury and the reduction in the full benefit of myocardial reperfusion. Remote ischemic preconditioning, perconditioning, and postconditioning have all been shown to be cardioprotective. However, it is still unknown which one is the most beneficial. To examine this issue, we used adult male Wistar rat ischemia reperfusion models to compare the cardioprotective effect of these three approaches applied on double-sided hind limbs. METHODS: The rats were randomly distributed to the following five groups: sham, ischemia reperfusion, remote preconditioning, remote perconditioning, and remote post-conditioning. The ischemia/reperfusion model was established by sternotomy followed by a 30-min ligation of the left coronary artery and a subsequent 3-h reperfusion. Remote conditioning was induced with three 5-min ischemia/5-min reperfusion cycles of the double-sided hind limbs using a tourniquet. RESULTS: A lower early reperfusion arrhythmia score (1.50 + 0.97) was found in the rats treated with remote perconditioning compared to those in the ischemia reperfusion group (2.33 + 0.71). Meanwhile, reduced infarct size was also observed (15.27 + 5.19% in remote perconditioning, 14.53 + 3.45% in remote preconditioning, and 19.84+5.85% in remote post-conditioning vs. 34.47 + 7.13% in ischemia reperfusion, p<0.05), as well as higher expression levels of the apoptosis-relevant protein Bcl-2/Bax following global (ischemia/reperfusion) injury in in vivo rat heart models (1.255 + 0.053 in remote perconditioning, 1.463 + 0.290 in remote preconditioning, and 1.461 +0.541 in remote post-conditioning vs. 1.003 + 0.159 in ischemia reperfusion, p<0.05). CONCLUSION: Three remote conditioning strategies implemented with episodes of double-sided hind limb ischemia/reperfusion have similar therapeutic potential for cardiac ischemia/reperfusion injury, and remote perconditioning has a greater ability to prevent reperfusion arrhythmia.


Subject(s)
Animals , Male , Rats , Ischemic Preconditioning, Myocardial/methods , Myocardial Reperfusion Injury/therapy , Arrhythmias, Cardiac/physiopathology , Myocardial Infarction/prevention & control , Random Allocation , Rats, Wistar , Time Factors , Treatment Outcome , Ventricular Function/physiology
9.
Clinics ; 67(11): 1281-1283, Nov. 2012. ilus, tab
Article in English | LILACS | ID: lil-656718

ABSTRACT

OBJECTIVE: Ventricular septal defects resulting from post-traumatic cardiac injury are very rare. Percutaneous closure has emerged as a method for treating this disorder. We wish to report our experience in three patients who underwent percutaneous closure of a post-traumatic ventricular septal defect with a patent ductus arteriosus occluder. METHODS: We treated three patients with post-traumatic ventricular septal defects caused by stab wounds with knives. After the heart wound was repaired, patient examinations revealed ventricular septal defects with pulmonary/systemic flow ratios (Qp/Qs) of over 1.7. The post-traumatic ventricular septal defects were closed percutaneously with a patent ductus arteriosus occluder (Lifetech Scientific (Shenzhen) Co., LTD, Guangdong, China) utilizing standard techniques. RESULTS: Post-operative transthoracic echocardiography revealed no residual left-to-right shunt and indicated normal ventricular function. In addition, 320-slice computerized tomography showed that the occluder was well placed and exhibited normal morphology. CONCLUSION: Our experiences indicate that closure of a post-traumatic ventricular septal defect using a patent ductus arteriosus occluder is feasible, safe, and effective.


Subject(s)
Adolescent , Adult , Humans , Young Adult , Ductus Arteriosus, Patent/surgery , Heart Injuries/surgery , Heart Septal Defects, Ventricular/surgery , Septal Occluder Device , Echocardiography , Heart Septal Defects, Ventricular/etiology , Heart Ventricles/injuries , Treatment Outcome , Wounds, Stab/complications
10.
Article in Chinese | WPRIM | ID: wpr-349800

ABSTRACT

The mobilization efficiency of granulocyte colony-stimulating factor (G-CSF) and stem cell factor (SCF) to bone marrow mononuclear cells (MNCs) in mice was observed,and the changes of CXCL12/CXCR4 signal were detected in order to find out the mobilization mechanism of stem cells.Kunming mice were randomly divided into two groups.The mice in treatment group were subjected to subcutaneous injection of G-CSF at a dose of 100 μg/kg and SCF at a dose of 25 μg/kg every day for 5 days,and those in control group were given isodose physiological saline.The MNCs were separated,counted and cultured,and the colony-forming unit-fibroblast (CFU-F) was evaluated.CD34+CXCR4+ MNCs were sorted by flow cytometry.The expression of CXCL12 protein in bone marrow extracellular fluid was detected by ELISA,and that of CXCL12 mRNA in bone marrow was measured by RT-PCR.The results showed that the counts of MNCs in peripheral blood and bone marrow were increased after administration of G-CSF/SCF (P<0.01).The factors had a dramatic effect on the expansion capability of CFU-F (P<0.05).Flow cytometric of bone marrow MNCs surface markers revealed that CD34+CXCR4+ cells accounted for 44.6%±8.7% of the total CD34+ MNCs.Moreover,G-CSF/SCF treatment induced a decrease in bone marrowCXCL12 mRNA that closely mirrored the fall in CXCL12 protein.In this study,it is evidenced that G-CSF/SCF can effectively induce MNCs mobilization by disrupting the balance of CXCL12/CXCR4 signaling pathway in the bone marrow and down-regulating the interaction of CXCL12/CXCR4.

11.
Article in English | WPRIM | ID: wpr-236221

ABSTRACT

<p><b>OBJECTIVE</b>To observe the clinical effect of combined chemotherapy and Chinese herbal medicine in treating colonic cancer.</p><p><b>METHODS</b>One hundred and sixty-three patients were assigned, according to their will, to two groups, 105 in the traditional Chinese medicine treated group (Group A) and 58 in the combined treatment group (Group B). The Chinese herbal drug Zhao's Weitiao No. 3 ( 3, ZW3) was given to both groups, twice a day, 40 mL each time, 30 days as one cycle, and over 6 cycles applied in total. For patients in Group B, the chemotherapy of OLF protocol (L-OHP+LV+5-FU) was given for 4-6 cycles. The effects of treatment on the main symptoms, tumor mass, patients' quality of life (QOL) and body weight, changes of carcino-embryonic antigen (CEA), as well as the integral effect and survival rate were observed and compared.</p><p><b>RESULTS</b>The total effective rate in Group A and Group B was 89.52% and 86.21% respectively, on the main clinical symptoms; 86.67% and 93.10% on tumor mass, 82.86% and 77.59% on QOL, 85.71% and 75.86% on body weight and 76.19% and 79.31% on CEA. The integral efficacy of total beneficial rate was 73.33% and 68.97%; and the 3-year survival rate 49.52% and 46.65% in Group A and Group B. These data showed that the effect in Group A was better than in Group B in terms of clinical symptom improvement, QOL, body weight and integral beneficence increase and survival rate, though it was inferior in reducing the tumor mass and CEA level.</p><p><b>CONCLUSION</b>Chinese drug ZW3 for the treatment of colonic cancer could improve the main clinical symptoms, improve the QOL, increase body weight and prolong the survival time of patients, showing a favorable integral effect.</p>


Subject(s)
Adenocarcinoma , Diagnosis , Drug Therapy , Mortality , Adult , Aged , Aged, 80 and over , Antineoplastic Agents, Phytogenic , Therapeutic Uses , Antineoplastic Combined Chemotherapy Protocols , Therapeutic Uses , Colonic Neoplasms , Diagnosis , Drug Therapy , Mortality , Combined Modality Therapy , Drugs, Chinese Herbal , Therapeutic Uses , Female , Fluorouracil , Humans , Male , Medicine, Chinese Traditional , Methods , Middle Aged , Organoplatinum Compounds , Quality of Life , Survival Analysis , Treatment Outcome
12.
J Genet ; 2007 Dec; 86(3): 269-76
Article in English | IMSEAR | ID: sea-114455

ABSTRACT

Genetic factors play a significant role in influencing the variation of age at natural menopause (AANM). Estrogen receptor beta (ESR2), is an important factor in the mechanism of action of estrogen, while the aromatase gene (CYP19) and the 17-alpha-hydroxylase gene (CYP17) are involved in the biosynthesis of estrogen. We tested whether polymorphisms of ESR2, CYP19 and CYP17 genes are associated with AANM in Caucasian females. A total of 52 SNPs (17 for ESR2, 28 for CYP19, and 7 for CYP17) were successfully genotyped for 229 Caucasian women having experienced natural menopause. Comprehensive statistical analyses focusing on the association of these genes with AANM were conducted. The effects of age, height and age at menarche on AANM were adjusted when conducting association analyses. We found that six SNPs (2, 6-7, 9, 13 and 16) within ESR2 were not significantly associated with AANM after Bonferroni correction. However, two blocks of ESR2 were associated with AANM. For CYP19, two SNPs (24 and 27) were nominally associated with AANM. No significant association was observed between CYP17 and AANM. Our results suggest that genetic variation in the ESR2 and CYP19 genes may influence the variation in AANM in Caucasian women.


Subject(s)
Age Factors , Aromatase/genetics , Base Sequence , Estrogen Receptor beta/genetics , Estrogens/metabolism , Female , Humans , Linkage Disequilibrium , Menopause/genetics , Middle Aged , Polymorphism, Single Nucleotide , Signal Transduction/genetics , Steroid 17-alpha-Hydroxylase/genetics
13.
Article in Chinese | WPRIM | ID: wpr-268095

ABSTRACT

<p><b>OBJECTIVE</b>To observe the changes in the activity of dendritic cells (DCs) after carcino-embryonic antigen (CEA) gene transfection mediated by recombinant adeno-associated virus type2 (rAAV) and tumor cell lysate.</p><p><b>METHODS</b>Immature DCs isolated from peripheral blood monocytes of HLA-A11-positive healthy volunteers were infected with the rAAV carrying CEA gene or loaded with tumor cell lysate. The surface markers of the DCs such as CD40, CD 1alpha, and CD86 were analyzed by flow cytometry. Interleukin-12 (IL-12) in the supernatants of DCs and interferon-gamma (IFN-gamma) released by the cytotoxic T lymphocytes (CTLs) were determined by ELISA detection kit. The specific killing activity of CTL against LoVo cells was assessed by MTT assay.</p><p><b>RESULTS</b>The DCs following antigen loading with the two methods both highly expressed CD40, CD86 and IL-12, and induced specific CTL that specifically recognized and killed LoVo cells, but the killing effect resulting from rAAV infection of the DCs was much better than that induced by tumor cell lysate loading.</p><p><b>CONCLUSION</b>Both methods of antigen loading can induce mature DCs from peripheral blood monocyte cells, but rAAV infection of the DCs can be more effective than tumor cells lysate loading. DCs infected with rAAV may have the potential to serve as an adjuvant immunotherapy for patients with colorectal carcinoma.</p>


Subject(s)
B7-2 Antigen , Metabolism , CD40 Antigens , Metabolism , Cancer Vaccines , Allergy and Immunology , Carcinoembryonic Antigen , Genetics , Cell Line, Tumor , Colorectal Neoplasms , Therapeutics , Dendritic Cells , Allergy and Immunology , Metabolism , Dependovirus , Genetics , Genetic Vectors , Humans , Interleukin-12 , Metabolism , Transfection
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