ABSTRACT
Objective To analyze and evaluate the items of the QLICD-CPHD(V2.0)scale for chronic pulmonary heart disease using classical test theory(CTT)and item response theory(IRT).Methods 184 patients with chronic pulmonary heart disease were investigated by QLICD-CPHD(V2.0)scale.The items of the QLICD-CPHD(V2.0)scale was evaluated by some statistical methods based on CTT including correlation coefficient method,variance method,factor analysis method and Cronbach's α coefficient method.Meanwhile,Samejima hierarchical response model of item response theory was utilized to calculate the difficulty,information and differentiation coefficient content of each item in the scale.Results CTT results showed that 7 items failed meet at least three or more statistical requirements,including 6 items in the general module and 1 item in the disease specific module.IRT results showed that the range of item differentiation was 1.18～1.44,which was suitable.The difficulty coefficient increases monotonously with the increase of the difficulty level(B1→B4),and some items exceed the standard value range.The average information amount of each item ranges from 0.185～0.576.Conclusion By CTT and IRT analysis,most items of the QLICD-CPHD(V2.0)scale have good performance and good differentiation,but a few items still need further revision.
ABSTRACT
Objective To explore the potential mechanism of abnormal behavior resulted from maternal separation in neo-natal period in rat. Methods Neonatal rats were equally and randomly divided into maternal separation group and control group. The rats in maternal separation group were separated from the dam for 3h per day on postnatal days (PND) 2 to 21, nothing was done to the rats in the control group. The brain tissues were taken out after being killed on PND 7, 14, and 21. The expressions of Caveolin-l, BDNF and GFAP in hippocampal formation were detected by immunohistochemistry. Semiquantitative assessment of immunohistochemical images was performed by Image-Pro Plus software. Results Compared with control groups, the expres-sion of Caveolin-l on PND 7 had no signiifcant change, while BDNF and GFAP were signiifcantly increased in maternal separa-tion group (P<0.05). On PND 14 and 21, the expressions of Caveolin-l, BDNF and GFAP were signiifcantly decreased in maternal separation group (P<0.05). Conclusions Decreased expressions of Caveolin-l, BDNF and GFAP caused by maternal separation in neonatal period may be associated with abnormal behaviors in adulthood in rat.