ABSTRACT
Objective To evaluate the feasibility of biodegradable flow-diverting stent for treatment of intracranial wide-neck aneurysm.Methods Twenty adult New Zealand rabbits were used to induce wide-neck aneurysm models by elastase,and then,these models were divided into 3 groups:in group one (n=7),biodegradable polyglycolic acid flow-diverting stents (PGA-FDs) were implanted into parent arteries and covered the necks of aneurysms;in group 2 (n=7),the bare metal FDs replaced PGA-FDs;the observation period of animals in group one and group 2 was 3 months;in group 3 (n=6),the PGA-FDs were implanted into parent arteries with 6 weeks of observation period.The status of aneurysm occlusion and patency of side branches covered by the stent were assessed,and all animals were sacrificed:HE staining was used to observe the thrombosis in the aneurysms,Elastic fiber staining was used to observe the neointimal thickness of parent arteries,and immuohistochemical staining was employed to detect the expressions of CD34,CD68 and alpha-actin (αSMA) in the parent arteries.Results Complete aneurysm occlusion rate in groups one,2 and 3 was 83.3％ (5/6),0％ (0/6) and 66.7％ (4/6),respectively.The neointimal thickness in parent artery of groups one,2 and 3 was (111±69) μm,(70±48) μm and (190±52) μm,respectively;that in group 3 was significantly higher than that in group one and 2 (P＜0.05).The neointima comprised predominantly smooth muscle cells and collagen fibers.Some CD34 positive vascular endothelial cells could be seen in the neointima of parent arteries of groups one and 2.Conclusion The PGA-FD is an effective device to occlude aneurysms without inducing obvious neointimal thickness and side branch stenosis in the 3-month follow-up.
ABSTRACT
Objective To evaluate the safety of biodegradable flow-diverting devices (FDs) for treatment of intracranial aneurysms in rabbits.Methods Biodegradable polyglycolic acid FDs (PGA-FDs) were implanted into the abdominal aorta in 12 New Zealand rabbits,covering the ostium of a lumbar artery.Animals underwent angiography at 3,6,and 12 weeks after device implantation;and 3 animals were sacrificed at 3 and 6 weeks for pathology by HE staining,Veigert resorcinol (VG) staining,and CD34,CD68 and α smooth muscle actin (αSMA) immunohistochemistry.The diameter of abdomen arota and the patency of side branches covered by the devices were assessed.All animals were sacrificed after angiography for pathology at 12 weeks.Results No side branch occlusions were noted,either immediately after implantation or at follow-ups.Abdominal aorta neointimal hyperplasia was minimal,and there were no differences immediately after device implantation,or at 3,6,and 12 weeks after device implantation (abdominal aorta diameter:[3.35±0.24] mm,[3.33 ±0.12] mm,[3.23±0.16] mm,[3.12±0.10] mm and [3.30±0.11] mm).Pathology results showed the neointima covered the strips of PGA-FD,and minial inflammation was abserved around the strips.The PGA strips were obsorbed completely at 12 weeks follow up with no apparent inflammation.The neointima predominantly comprised of smooth muscle cells and collagen fibers.Conclusion The PGA-FD is safe for abdominal aorta and normal side branches at short-term follow-up.
ABSTRACT
Objective To investigate the changes of serum procalcitonin (PCT), interleukin-6 (IL-6) and C reactive protein (CRP) in children with Henoch-Sch?nlein purpura (HSP) combined with surgical complications. Methods From January 2010 to June 2013, 48 children with HSP combined surgical complications were enrolled, the fasting venous blood were obtained in day 1, 3, 5 of admission and before discharge. Meanwhile, 30 healthy children were selected as control. Results Compared with the control group, the levels of serum PCT and IL-6 in day 1, 3, 5 of admission, and the levels of serum CRP in day 1, 3 of admission were higher in children with HSP combined with surgical complications (all P<0.01). The differences of levels of serum PCT, CRP and IL-6 in day 1, 3, 5 of admission and before discharge were statistically signiifcant between surgical group and non-surgical group. All these indicators showed a gradual downward trend. Compared with surgical group, the levels of serum PCT and IL-6 in day 3, 5 of admission and serum CRP in day 5 of admission were signiifcantly lower in non-surgical group (P<0.05). There were positive linear correlations between the level of serum PCT and IL-6, and between the level of PCT and CRP (r=0.48, 0.62, P<0.01). Conclusions The changes of serum PCT, IL-6 and CRP may be associated with the occurrence of surgical complications in children with HSP, monitor these indicators may help to assess the condition and to guide the treatment.