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Braz. j. med. biol. res ; 43(1): 85-95, Jan. 2010. ilus
Article in English | LILACS | ID: lil-535638

ABSTRACT

The objective of the present study was to determine whether lesion of the subthalamic nucleus (STN) promoted by N-methyl-D-aspartate (NMDA) would rescue nigrostriatal dopaminergic neurons after unilateral 6-hydroxydopamine (6-OHDA) injection into the medial forebrain bundle (MFB). Initially, 16 mg 6-OHDA (6-OHDA group) or vehicle (artificial cerebrospinal fluid - aCSF; Sham group) was infused into the right MFB of adult male Wistar rats. Fifteen days after surgery, the 6-OHDA and SHAM groups were randomly subdivided and received ipsilateral injection of either 60 mM NMDA or aCSF in the right STN. Additionally, a control group was not submitted to stereotaxic surgery. Five groups of rats were studied: 6-OHDA/NMDA, 6-OHDA/Sham, Sham/NMDA, Sham/Sham, and Control. Fourteen days after injection of 6-OHDA, rats were submitted to the rotational test induced by apomorphine (0.1 mg/kg, ip) and to the open-field test. The same tests were performed again 14 days after NMDA-induced lesion of the STN. The STN lesion reduced the contralateral turns induced by apomorphine and blocked the progression of motor impairment in the open-field test in 6-OHDA-treated rats. However, lesion of the STN did not prevent the reduction of striatal concentrations of dopamine and metabolites or the number of nigrostriatal dopaminergic neurons after 6-OHDA lesion. Therefore, STN lesion is able to reverse motor deficits after severe 6-OHDA-induced lesion of the nigrostriatal pathway, but does not protect or rescue dopaminergic neurons in the substantia nigra pars compacta.


Subject(s)
Animals , Male , Rats , Dopamine/physiology , Motor Activity/drug effects , Neurons/pathology , Parkinson Disease, Secondary/pathology , Substantia Nigra/cytology , Subthalamic Nucleus/injuries , Immunohistochemistry , Motor Activity/physiology , N-Methylaspartate , Neurons/drug effects , Neurons/physiology , Pharmaceutical Vehicles , Parkinson Disease, Secondary/chemically induced , Parkinson Disease, Secondary/physiopathology , Random Allocation , Rats, Wistar , Substantia Nigra/physiopathology , Subthalamic Nucleus/drug effects , Subthalamic Nucleus/pathology , Subthalamic Nucleus/surgery , /metabolism
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