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1.
China Pharmacy ; (12): 206-212, 2021.
Article in Chinese | WPRIM | ID: wpr-862645

ABSTRACT

OBJECTIVE:To establish characteristic chromatogram of Cornus officinalis and its different wine-processedproducts,investigate the differences of chromaticity values,and analyze them with chemical pattern recognition technology.METHODS:UPLC method was adopted. Using loganin as reference,UPLC characteristic chromatograms were drawn for 10batches of C. officinalis and 20 batches of different wine-processed products (stewing with wine,steaming with wine). TCMFingerprint Similarity Evaluation System(2012A edition)was used for similarity evaluation,and common peaks were confirmed.The chromaticity values [lightness(L),red and green tone value(a),yellow and blue tone value(b),color difference value(ΔE)]were determined by spectrophotometer. SPSS 20.0 and SIMCA 14.0 software were used for cluster analysis,principal componentanalysis and partial least squares-discriminant analysis;taking the area of characteristic peak and chromaticity value as indexes,andthe variable importance projection greater than 1 as the standard,the difference markers affecting its quality were screened.RESULTS:There were 6 common peaks in the chromatograms for decoction piece of C. officinalis,7 common peaks forwine-processed C. officinalis(stewing with wine)and wine-processed C. officinalis(steaming with wine). Four components wereidentified as gallic acid,5-hydroxymethylfurfural,morroniside,loganin. 5-hydroxymethylfurfural was produced after processing.The similarity between C. officinalis and different wine-processed products (stewing and steaming with wine) was low(0.869-0.937,0.845-0.944),but the similarity between different wine-processed products was higher than 0.99. ΔL,Δa,Δb and ΔEof C. officinalis decoction pieces and wine-processed C. officinalis decoction pieces(stewing in wine)were -9.42--3.58,-24.92- -15.00,-11.33- -7.00 and 17.01-28.12,respectively. ΔL,Δa,Δb and ΔE of C. officinalis decoction pieces and wine-processed C. officinalis(steaming in wine)decoction pieces were -8.58--2.42,-25.08--13.83,-10.92--6.08,15.58-28.67. ΔL,Δa,Δb and ΔE of wine-processed C. officinalis decoction pieces(stewing and steaming with wine)were -2.17-3.00,-0.75-2.50, 0.25-1.42 and 1.25-3.83,respectively. Results of cluster analysis showed that 30 batches of sample were clustered into two categories,S1-S10 were clustered into one category,and S11-S30 were clustered into other category. Principal component analysis showed that cumulative contribution rate of former two main components was 83.147%. Results of partial least squares-discriminant analysis showed that morroniside,No.5 peak and chromaticity values(L,a,b)were the difference markers affecting its quality. CONCLUSIONS:Established UPLC characteristic chromatogram is stable and feasible,and can be used to rapidly identify C. officinalis and its different wine-processed products. Established chemical mode can be used to identify different wine-processed products.

2.
China Pharmacy ; (12): 56-63, 2021.
Article in Chinese | WPRIM | ID: wpr-862266

ABSTRACT

OBJECTIVE:To provide reference for the identification and proces sing end-point determination of raw Morus alba and its processed products (honey-processed M. alba ). METHODS :UPLC method was adopted. The determination was performed on Waters BEH Shield RP C 18 column with mobile phase consisted of acetonitrile- 0.1% phosphoric acid solution (gradient elution ) at the flow rate of 0.30 mL/min. The column temperature was set at 30 ℃. The program wavelengths were set at 280 nm(0-4 min) and 320 nm(4-35 min). Similarity Evaluation System for Chromatogram Fingerprint of TCM (2012 edition)was used to establish UPLC fingerprint and carry out similarity evaluation of 13 batches of M. alba and honey-processed M. alba . The chromatographic peaks were identified with reference substance fingerprint. The colorimetric value (L,a,b) of 13 batches of M. alba and honey-processed M. alba powder were determined ,and average total colorimetric value (E)was calculated. OPLS-DA and cluster analysis were adopted to analyze the differences in fingerprints and colorimetric values of M. alba before and after processing. At the same time ,the dynamic change rule of fingerprint and colorimetric value of honey-processed M. alba at different processing time points were analyzed to determine the processing end-point. RESULTS :There were obvious differences in fingerprints before and after processing ,and the similarity of 13 batches of M. alba and honey-processed M. alba were all higher than 0.9. Totally 21 common peaks were calibrated for M. alba ,and 23 common peaks for honey-processed M. alba ;peak 1 and peak 2 were newly produced compounds of honey-processed M. alba . Peak 2,peak 7,peak 14 and peak 19 were identified as 5-hydroxymethylfurfural, mulberry glucoside A ,oxidized resveratrol ,mulberry flavonoids G. Results of OPLS-DA showed that the peak area-sample quantity ratio of peak 1,peak 2,peak 18,peak 20 and the chromaticity values (L,a,b)were the most important factors affecting the difference of raw and processed products of M. alba . When the E ranged 75.84-80.88 as the processing end-point of honey-processed M. alba ,the processing time was determined as 22-34 min. CONCLUSIONS : The established UPLC fingerprint and colorimetric value determination method can be used to identify the raw and processed products of M. alba as well as determine the processing end-point of honey-processed M. alba .

3.
Article in Chinese | WPRIM | ID: wpr-879046

ABSTRACT

The extract rates, multicomponent content and fingerprint were determined in this study to investigate the quality diffe-rence between standard decoction of raw Paeoniae Radix Alba and fried Paeoniae Radix Alba. UPLC fingerprint was established for 17 batches of standard decoction of raw and fried Paeoniae Radix Alba, and the contents of gallic acid, catechin, albiflorin, paeoniflorin and benzoyl paeoniflorin were determined. The peak areas of standard decoction were analyzed by the independent t-test and orthogonal partial least squares discriminant analysis. There was no significant difference in extract rates between the standard decoction of raw and fried Paeoniae Radix Alba. After fried processing, the content of albiflorin increased by 0.26%, while the contents of gallic acid, catechin, paeoniflorin and benzoyl paeoniflorin decreased by 13.04%, 27.97%, 10.30% and 18.79% respectively. There were 14 common peaks in the fingerprint of standard decoction of raw Paeoniae Radix Alba, and 16 common peaks in the fried Paeoniae Radix Alba. Peak 1 and peak 3 were new ones after processing, among which the peak 3 was 5-hydroxymethylfurfural. The results showed that peak 1, peak 3, peak 11 and peak 15 were the key compounds to distinguish standard decoction of raw and fried Paeoniae Radix Alba. In conclusion, this method is stable and can be used for the study of quantity transfer and quality control in the preparation process of standard decoction, granules and other dosage forms for raw and fried Paeoniae Radix Alba, providing reference for the identification of raw and fried Paeoniae Radix Alba and related preparations.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Paeonia , Quality Control , Reference Standards
4.
China Pharmacy ; (12): 1466-1472, 2021.
Article in Chinese | WPRIM | ID: wpr-881283

ABSTRACT

OBJECTIVE:To study the co rrelation of the chro maticity value of Schizonepeta tenuifolia charcoal of different processing time(0-40 min,similarly herein after)with multiple indicators ,and to reveal the quality change law of S. tenuifolia charcoal during processing and confirm the terminal time. METHODS :The contents of ethanol-soluble extracts from S. tenuifolia charcoal decoction pieces of different processing time were determined. UPLC fingerprint of S. tenuifolia decoction pieces and S. tenuifolia charcoal decoction pieces of different processing time were established ,and the similarity evaluation was also conducted. The chromatographic peaks were confirmed by comparison with substance control. The same UPLC conditions were used to determine the contents of index components (hesperidin,rosmarinic acid ,menthone)in S. tenuifolia charcoal decoction pieces of different processing time. The colorimetric method was used to measure the chromaticity value of S. tenuifolia charcoal decoction pieces of different processing time. Meanwhile ,sample of processing 0 min was used as a control to calculate the total color value (E)and the total color difference value (ΔE). Pearson correlation analysis ,cluster analysis and orthogonal partial least squares discriminant analysis (OPLS-DA)were performed on the ethanol-soluble extracts ,index component contents ,chromatographic peak area and chromaticity value. The terminal time of processing S. tenuifolia charcoal was conf irmed,and validation test was also conducted. RESULTS :With the extension of processing time , the content of ethanol-soluble extract in S. tenuifolia charcoal qq.com decoction pieces gradually decreased. A total of 17 chromato- graphic peaks were identified in 12 batches of S. tenuifolia decoction piece ,and its si milarity with the control fingerprint was greater than 0.9. 21 chromatographic peaks were identified in S. tenuifolia charcoal decoction pieces of different processing time,and its similarity with the chromatogram of sample of processing 0 min decreased with the processing time ,and the similarity after 18 min was lower than 0.9. The chromatographic peak 9 was hesperidin ,peak 10 was rosmarinic acid and peak 17 was menthone. The determination of content and chromaticity value showed that with the extension of processing time ,the contents of hesperidin ,rosmarinic acid and menthone decreased gradually ;the color L,b and E values of S. tenuifolia charcoal decoction piece powder decreased gradually ,and the a and ΔE values increased gradually. Pearson correlation analysis showed that the contents of ethanol-soluble extract ,hesperidin,rosmarinic acid and menthone ,the peak areas of 15 chromatographic peaks (peak 2,7-15,17-21)were significantly positively correlated with E value(P<0.01);the peak areas of 5 chromatographic peaks (peak 1,3-6)were significantly negatively correlated with E value(P<0.01),but peak area of peak 16 was not related to E value(P> 0.05). Results of cluster analysis showed that S. tenuifolia charcoal decoction pieces of different processing time were divided into 2 categories;the first category was processed for 0-16 min,and the second category was processed for 18-40 min. The results of OPLS-DA showed that the VIP values of peak 6 area(2.800 75),L value(2.327 54),peak 3 area(1.793 39),b value(1.735 78) and peak 5 area(1.244 04)were greater than 1. The final processing time of S. tenuifolia charcoal was 18 min. The results of validation experiment showed that the L,a and b values of S. tenuifolia charcoal decoction piece were 20.22-22.00,4.44-7.67, 9.78-13.00,and ΔE were 13.50-14.12,respectively. CONCLUSIONS :The chromaticity value of S. tenuifolia charcoal decoction pieces of different processing time is closely related to the contents of ethanol-soluble extract ,hesperidin,rosmarinic acid , menthone and the area of 20 chromatographic peaks. It is suggested that the terminal time of processing S. tenuifolia is 18 min.

5.
Acta Pharmaceutica Sinica ; (12): 2960-2967, 2020.
Article in Chinese | WPRIM | ID: wpr-862296

ABSTRACT

The goal of this work was to establish a population pharmacokinetics (PPK) model of tacrolimus in idiopathic membranous nephropathy (IMN) patients and to identify potential covariates that influence pharmacokinetic of tacrolimus. A total of 610 data points on the blood concentration of tacrolimus were collected from 96 IMN patients in routine clinical settings. Nonlinear mixed-effect modeling (NONMEM) was used to investigate the effects of CYP3A5 genotype, age, gender, weight, laboratory tests and co-therapy medications on the pharmacokinetic of tacrolimus. The PPK model was evaluated by the goodness-of-fit (GOT), bootstrap and prediction corrected visual predictive check (pc-VPC). The pharmacokinetic of tacrolimus was described by a one-compartment model. The apparent clearance (CL/F) of CYP3A5*1/*3 and *1/*1 were 1.57 and 1.86 times of that of *3/*3, respectively. The CL/F of tacrolimus was 73.6% in patients undergoing co-therapy with Wuzhi capsules, and 1.2 times than that of the patients undergoing co-therapy with Jinshuibao capsules. The evaluation of the model shows that the model is stable and has satisfactory predictive performance. The clinical trial was approved by the Society of Ethics and conducted in Binzhou Medical University Hospital. The established PPK model can describe the pharmacokinetic characteristics of tacrolimus in Chinese patients with IMN, and can facilitate individualized therapy with tacrolimus.

6.
Article in Chinese | WPRIM | ID: wpr-828425

ABSTRACT

The present study was performed to establish the UPLC fingerprints of Bolbostemmatis Rhizoma and determine the contents of three saponins by quantitative analysis of multi-components by single marker(QAMS), and provide basis for quality evaluation of Bolbostemmatis Rhizoma. The analysis was carried out on an analytical column of Waters Cortecs T3(2.1 mm×100 mm,1.6 μm)with gradient elution by acetonitrile-0.1% phosphoric acid solution, at a flow rate of 0.3 mL·min~(-1). The detection wavelength was 203 nm, the column temperature was 30 ℃ and the injection volume was 1 μL. The UPLC fingerprints of Bolbostemmatis Rhizoma were established and evaluated by similarity calculation, cluster analysis and principal component analysis. The relative calibration factors of toberoside B and toberoside C were determined with toberoside A as internal reference. The content was calculated by relative calibration factors to develop a method of QAMS. Comparing the results of QAMS with those of ESM, the accuracy and feasibility of one-eva-luation and multi-evaluation can be determined. RESULTS:: showed that the fingerprints of 19 batches of Bolbostemmatis Rhizoma have four common peaks with similarities ranging from 0.754 to 1.000. Cluster analysis and principal component analysis classified 19 batches of Bolbostemmatis Rhizoma into three categories, which was consistent with the similarity evaluation results. The relative deviation between the content of tubeicosides B and C in 19 batches of Bolbostemmatis Rhizoma determined by QAMS and ESM is less than 5.0%, indicating that there was no significant difference between the two methods. Therefore, the UPLC fingerprints combined with QAMS and similarity evaluation can be effectively used to evaluate the quality of Bolbostemmatis Rhizoma.


Subject(s)
Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Principal Component Analysis , Quality Control , Rhizome
7.
Article in Chinese | WPRIM | ID: wpr-871897

ABSTRACT

In recent years,with the change of lifestyle in China, hypertriglyceridemia (HTG) has been the second leading cause of acute pancreatitis (AP). Recent studies have shown that HTG has long-lasting influences on AP onset (including recurrence), AP severity, as well as pancreatic regeneration. However, the underlying mechanisms of HTG-AP is still unclear, mainly focusing on free fatty acid, microcirculation disturbance, calcium overload, oxidative stress, gene polymorphism and so on. However, there is lack of effective intervention for the diagnosis and treatment of HTG-AP, which needs further study.

8.
Article in Chinese | WPRIM | ID: wpr-843179

ABSTRACT

Objective • To verify the effectiveness of the intervention program of bowel dysfunction in patients with spinal cord injury (SCI). Methods • Eighty bowel dysfunction patients with SCI in a rehabilitation hospital in Shanghai from Jan. to Dec. 2018 were included. According to the time of admission, the patients were divided into intervention group and control group, with 40 cases in each group. The patients in the control group received the routine nursing, and the patients in the intervention group were provided with the intervention program constructed by this study. The bowel function indexes of the two groups were compared at the time of admission, 4 weeks after intervention and 1 month after discharge. The quality of life in the two groups was compared at the time of admission and 1 day before discharge. Results • After intervention, the defecation frequency, fecal character score, defecation time, abdominal distention, constipation rate and drug dependence rate of the intervention group were lower than those of the control group (all P<0.05), and their total scores of quality of life and the scores in various fields were higher than those of the control group (all P<0.05). The differences in the quality of life between the two groups of patients after intervention and at the time of admission were statistically significant except for the social field (all P<0.05). Conclusion • The intervention program for bowel dysfunction patients with SCI can effectively lead to the recovery of the bowel function, reduce the incidence of bowel complications, and improve the quality of their life.

9.
Article in Chinese | WPRIM | ID: wpr-805369

ABSTRACT

Objective@#To observe the changes of skin lesion, neuralgia and blood pressure in hypertension patients and non-hyperension patients before and after the early formal treatment on herpes zoster in the head and face.@*Methods@#Clinical data were collected from June 2015 to May 2018 in the Department of Dermatology, the First People's Hospital, Changshu, Jiangsu province. In 104 cases of hospitalized patients with herpes zoster, 46 cases had high blood pressure (hypertension group); 58 cases did not have high blood pressure (non-hypertension group). The skin lesion, numerical rating scale (NRS) and blood pressure changes were compared between the two groups before and after treatment.@*Results@#A total of 99 patients completed treatment; 45 cases in the hypertensive group included 25 males, 20 females, aged 35 to 71 (52.40±12.51) years; 54 cases in the non-hypertensive group included 30 males and 24 females, aged 36 to 75 (52.62±12.67) years. There was no statistically significant difference in the time of control, improving and healing of skin lesion (t=-0.457, -0.607 and 1.399, P>0.05) . There was no statistically significant difference in NRS between the two groups before treatment (t=-0.009, P=0.993 >0.05). After treatment, the NRS of both groups decreased compared with those before treatment, and the difference was statistically significant (F=151.130, 181.444, P<0.05). There was no significant difference in NRS between the two groups after treatment (Z=-0.032, -0.154, -0.149 and -0.426, P>0.05). In the hypertension group, the rate of blood pressure increase before treatment was 62.22%, and the blood pressure decreased after treatment compared with that before treatment. The difference was statistically significant (P<0.05). In the non-hypertension group, the rate of increase of blood pressure before treatment was 5.56%, and the blood pressure after treatment was lower than before treatment. The difference was not statistically significant before and after treatment (P>0.05).@*Conclusions@#Blood pressure can be increased when herpes zoster occurs. After early formal treatment of herpes zoster, skin lesion healed, neuralgia reduced and blood pressure can return to normal. The history of hypertension and the increase of blood pressurd before treatment has no effect on the recovery of skin lesion and neuralgia.

10.
Article in Chinese | WPRIM | ID: wpr-751871

ABSTRACT

Objective To investigate the value of the reverse shock index multiplied by GlasgowComa scale score (rSIG) and serum translocator protein 18000 in the prognosis of patients with severe traumatic brain injury. Methods One hundred and fifteen patients with severe traumatic brain injury were divided into the survival group and death group. SPSS 20.0 software was used to compare the vital signs, rSIG and TSPO between the two groups, and the relationship between rSIG and TSPO was analyzed. Receiver operating characteristic (ROC) curve was used to predict the value of rSIG and TSPO and their combination in the prognosis of patients with severe traumatic brain injury. According to the best cut-off value of rSIG and TSPO of ROC curve, patients were divided into the rSIG ≤ 14.8 group and rSIG>14.8 group, and the TSPO ≤ 1.84 ng/mL group and TSPO>1.84 ng/mL group, and the mortality between the groups was compared. Results In 115 patients, rSIG of the survival group was significantly higher than that of the death group, and TSPO was significantly lower than that of the death group [(10.5±4.4) vs. (6.4±4.1), 1.0(0.3,1.9) ng/mL vs.3.4 (2.0, 4.6) ng/mL, P<0.01]. The ability of rSIG combined with TSPO to forecast the mortality of patients with severe traumatic brain injury is not superior to the predictive power of these two indicators alone. The serum TSPO value and 28-day mortality in the rSIG > 4.15 group were significantly higher than those in the rSIG ≤ 4.15 group. The rSIG value of the TSPO ≤ 1.84 ng/mL group was significantly higher than that of the TSPO>1.84 ng/mL group; the 28-day mortality was significantly lower than that in the TSPO>1.84 ng/mL group. The rSIG value was negatively correlated with serum TSPO value (r=-0.611, P<0.01). Conclusions rSIG value and serum TSPO value have good predictive value for the prognosis of patients with severe traumatic brain injury, and can provide certain guiding significance in clinical practice.

11.
Article in Chinese | WPRIM | ID: wpr-797900

ABSTRACT

Objective@#To investigate the value of acoustic parameters in the voice therapy for patients with unilateral vocal cord paralysis (UVCP).@*Methods@#From May 2015 to April 2018, 51 patients with UVCP and 59 healthy controls in Department of Otorhinolaryngology Head and Neck Surgery, Tianjin First Central Hospital, were involved in this research retrospectively. The UVCP patients were diagnosed with stroboscopic laryngoscopy. The minimum glottal area (MGA) was calculated by KIPS software when the people were pronouncing/i:/. The fundamental frequency (F0), Jitter, Shimmer and NHR were detected by CSL4500 multiple acoustic voice analyzer.@*Results@#MGA of UVCP patients was much higher than that of healthy control (male: 433.68±64.52 vs. 294.41±51.82, t=9.23, P=0.000; female: 498.80±73.42 vs. 302.03±76.54, t=13.21, P=0.000), which meaned vocal cord insufficiency.After voice therapy, MGA reduced significantly (male: 288.48±55.09, female: 258.22±57.17, t=24.41 and 31.22, P=0.000 vs. pre-therapy). MGA of untreated patients decreased in varying degrees. Compared with the voice therapy group, the MGA decreased in a significantly lower extent (24.25±22.91 vs. 188.31±54.37, t=8.97, P=0.000). The F0, Jitter, Shimmer and NHR raised significantly in UVCP patients group (P=0.000 vs. healthy control group), and they were reduced by voice therapy (all P<0.05). Each of the four acoustic parameters was relative with MGA, r=0.551, 0.867, 0.853 and 0.875 in turn, P=0.001, 0.000, 0.000, and 0.000.@*Conclusion@#MGA and acoustic parameters can reflect the acoustic features of UVCP patients, which are useful tools in the UVCP assessment and voice therapy.

12.
Article in Chinese | WPRIM | ID: wpr-792195

ABSTRACT

Objective To verify the change of the anxiety and depression of patients anteriorposterior treatment at paraurethral duct dilitation after gonococcal infection of paraurethral duct.Methods Twenty-eight patients of paraurethral duct dilatation after male gonococcal infection of paraurethral duct were enrolled,and the patients were treated in the Department of Dermatology and Venerology,the First People's Hospital of Changshu City.The anxiety and depression of patients anteriorposterior treatment were assessed and compared with Hamilton depression scale and Hamilton anxiety scale.Results The total scores of Hamilton depression scale and Hamilton anxiety scale before treatment were positively correlated with course of disease (r =0.481,P =0.0095;r =0.549,P =0.0025).The total scores of Hamilton depression and anxiety before treatment were from 1 to 31 (18.9±7.5) and from 4 to 30 (14.9±6.8).The symptoms of depression and anxiety appeared in 11 patients and 15 patients respectively.The total scores of Hamilton depression andanxiety after treatment were from 0 to 8 (4.3±1.7) and from 0 to 8 (3.3±1.7).There was no symptom of depression and anxiety.The difference of the total score of Hamilton depression and anxiety before and after treatment had statistical significance (t =12.270,P<0.001,t =10.626,P<0.001).The difference of positive rate of depressive and anxious symptom before and after treatment had statistical significance too (x2 =9.05,P=0.003;x2 =13.06,P=0.000).Conclusions The sequelae of gonococcal paraurethral duct infection in male paraurethral duct dilatation can result in the anxiety and depression of patients,which should be treated.Effective therapy can relieve or cure the anxiety and depression of patients without the assistance of psychotropic drugs.

13.
Article in Chinese | WPRIM | ID: wpr-773182

ABSTRACT

To explore the medication rules of herbal prescriptions for nonalcoholic fatty liver disease,and analyze the possible drug targets and interactions,in order to explore the mechanisms of the herbs. Randomized controlled trials of herbal prescriptions for treating nonalcoholic fatty liver disease were collected from CNKI,Wan Fang,VIP,Sino Med and PubMed databases. The properties,flavors and meridian tropism of herbs were analyzed by using systematic cluster analysis method with SPSS 19. 0 software. Subsequently,the association rules of herbs were analyzed by using Clementine 12. 0 software. Finally,the interactions between targets and relevant signaling pathways were analyzed by Traditional Chinese Medicine Systems Pharmacology Database(TCMSP),Search Tool for the Retrieval of Interacting Genes/Proteins(STRING) and Kyoto Encyclopedia of Genes and Genomes(KEGG). In the 88 prescriptions screened out,the commonly used herbs were Salvia miltiorrhiza,Bupleurum chinense,Alisma orientale,and Crataegus pinnatifida,and the potential signaling pathways were PPAR signaling pathway and calcium signaling pathway. The results showed that the main effects of herbal prescriptions were to improve blood flow/clear blood stasis,clear heatiness/dampness,promote digestion and strengthen spleen. And its mechanism of action may be achieved through the regulation of PPAR signaling pathway and calcium signaling pathway.


Subject(s)
Data Mining , Drugs, Chinese Herbal , Therapeutic Uses , Humans , Medicine, Chinese Traditional , Meridians , Non-alcoholic Fatty Liver Disease , Drug Therapy , Randomized Controlled Trials as Topic , Signal Transduction
14.
Acta Pharmaceutica Sinica ; (12): 1982-1989, 2019.
Article in Chinese | WPRIM | ID: wpr-780281

ABSTRACT

Seven main components in eleutheroside were used as research objects, and the mechanism of action of total eleutheroside for treatment of diabetes mellitus type 2 was investigated by network pharmacology. The SwissTargetPrediction, GeneCard, and String platforms were used to predict the 35 potential targets of these 7 components that are related to diabetes mellitus type 2. Then we used cytoscape 3.6.1 to build a "component-target" network map and used the Networkanalyzer tool for topology analysis. Gene ontology (GO) enrichment analysis and KEGG pathway enrichment analysis were performed on the DAVID6.8 platform, and the "component-target-path" network map was constructed based on the enrichment results. Those components mainly used in diabetes mellitus type 2 were screened as core components, and the core components were docked with key disease target proteins to verify the potential mechanism of the total eleutheroside. After screening, 8 important pathways associated with diabetes mellitus type 2 were identified. This study showed that eleutheroside A, eleutheroside D, eleutheroside E and sesamin played key roles in insulin resistance, apoptosis and inflammation pathways. The total eleutheroside may ameliorate type 2 diabetes mainly through regulating signal transducer and activator of transcription factors (STATs), non-receptor protein tyrosine phosphatase (PTPN) 1, PTPN2, c-Jun N-terminal kinase (JNK), and p38 mitogen activate protein kinase. These components worked together through multiple signaling pathway. Based on our data, eleutheroside is proposed as a novel therapeutic strategy for treatment of type 2 diabetes.

15.
Article in Chinese | WPRIM | ID: wpr-779452

ABSTRACT

Objective To investigate the effect of genetic variants in STAT4 and its interaction with exercise on the pathological characteristics of patients with liver cancer. Methods In the 601 new patients with primary liver cancer,

16.
Article in Chinese | WPRIM | ID: wpr-777932

ABSTRACT

Objective To investigate the influencing factors for liver cancer by gender in Shunde region, and to explore the potential interactions among influencing factors for liver cancer in males. Methods The relative excess risk of interaction (RERI) and other indices were used to evaluate the pair-wise interaction, and the classification and regression tree (CART) model was applied to explore the potential multi-factors interaction. Results This study included 1 037 male cases and 1 069 controls, together with 166 female cases and 185 controls. Chronic hepatitis B virus infection (CHB) and family history of liver cancer were significantly associated with increased risk of liver cancer both in males and females (both P<0.001). In males, positive additive interactions were observed between CHB and smoking or alcohol drinking, as well as between smoking and drinking. The RERI for CHB and smoking was 121.90(95% CI:52.85%-190.95%). Negative additive interactions were observed between exercise and CHB or smoking. Further, the CART analysis suggested that the CHB males who smoked and drank alcohol had the highest risk of liver cancer. Conclusions CHB and family history of liver cancer are important risk factors for liver cancer in both males and females. CHB, smoking, and alcohol drinking synergistically promote the incidence of liver cancer for males. Exercise can antagonize the hepatocarcinogenic effect of CHB and smoking.

17.
Chinese Medical Journal ; (24): 883-888, 2019.
Article in English | WPRIM | ID: wpr-772179

ABSTRACT

BACKGROUND@#Gestational diabetes mellitus (GDM) is usually diagnosed between 24th and 28th gestational week using the 75-g oral glucose tolerance test (OGTT). It is difficult to predict GDM before 24th gestational week because fast plasma glucose (FPG) decreases as the gestational age increases. It is controversial that if FPG ≥5.1 mmol/L before 24th gestational week should be intervened or not. The aim of this study was to evaluate the value of FPG to screen GDM before 24th gestational week in women with different pre-pregnancy body mass index (BMI).@*METHODS@#This was a multi-region retrospective cohort study in China. Women who had a singleton live birth between June 20, 2013 and November 30, 2014, resided in Beijing, Guangzhou and Chengdu, and received prenatal care in 21 selected hospitals, were included in this study. Pre-pregnancy BMI, FPG before the 24th gestational week, and one-step GDM screening with 75 g-OGTT at the 24th to 28th gestational weeks were extracted from medical charts and analyzed. The pregnant women were classified into four groups based on pre-pregnancy BMI: Group A (underweight, BMI < 18.5 kg/m), Group B (normal, BMI 18.5-23.9 kg/m), Group C (overweight, BMI 24.0-27.9 kg/m) and Group D (obesity, BMI ≥28.0 kg/m). The trend of FPG before 24th week of gestation was described, and the sensitivity and specificity of using FPG before the 24th gestational week to diagnose GDM among different pre-pregnancy BMI groups were reported. Differences in the means between groups were evaluated using independent sample t-test and analysis of variance. Pearson Chi-square test was used for categorical variables.@*RESULTS@#The prevalence of GDM was 20.0% (6806/34,087) in the study population. FPG decreased gradually as the gestational age increased in all pre-pregnancy BMI groups until the 19th gestational week. FPG was higher in women with higher pre-pregnancy BMI. FPG before the 24th gestational week and pre-pregnancy BMI could be used to predict GDM. The incidence of GDM in women with FPG ≥5.10 mmol/L in the 19th to 24th gestational weeks and pre-pregnancy overweight or obesity was significantly higher than that in women with FPG ≥5.10 mmol/L and pre-pregnancy BMI <24.0 kg/m (78.5% [62/79] vs. 52.9% [64/121], χ = 13.425, P < 0.001).@*CONCLUSIONS@#FPG decreased gradually as the gestational age increased in all pre-pregnancy BMI groups until the 19th gestational week. Pre-pregnancy overweight or obesity was associated with an increased FPG value before the 24th gestational week. FPG ≥5.10 mmol/L between 19 and 24 gestational weeks should be treated as GDM in women with pre-pregnancy overweight and obesity.


Subject(s)
Adult , Blood Glucose , Body Mass Index , Diabetes, Gestational , Blood , Diagnosis , Epidemiology , Fasting , Blood , Female , Gestational Age , Glucose Tolerance Test , Humans , Incidence , Pregnancy , Prevalence , ROC Curve , Retrospective Studies
18.
Chinese Journal of Dermatology ; (12): 670-672, 2018.
Article in Chinese | WPRIM | ID: wpr-710449

ABSTRACT

Objective To evaluate the therapeutic effect of combined glucocorticoids on herpes zoster of the head and face in the elderly aged ≥ 60 years.Methods From June 2013 to June 2017,76 patients with herpes zoster of the head and face were enrolled from the Department of Dermatology of Changshu No.1 People's Hospital,and randomly and equally divided into glucocorticoid group and conventional treatment group by drawing lots.The patients in the 2 groups were all treated with intravenous penciclovir (0.25 g,twice a day) for 7 days,intravenous mecobalamine (1 ml,once a day) for 7 days,and topical penciclovir cream four times every day,and the patients in the glucocorticoid group were additionally treated with intravenous methylprednisolone sodium succinate (40 mg,once a day) for 5 days.The time to skin lesion improvement and pain relief,incidence of postherpetic neuralgia and adverse reactions were compared between the two groups.Results The time to skin lesion improvement was shorter in the glucocorticoid group than in the conventional treatment group(P < 0.01).Before the treatment,no significant difference in the numerical rating scale (NRS) score for pain was observed between the 2 groups (P > 0.05).Seven days after the treatment,the NRS score was significantly lower in the glucocorticoid group than in the conventional treatment group (P < 0.05),while there was no significant difference between the 2 groups after 30-and 90-day treatment (both P > 0.05).The incidence rate of postherpetic neuralgia was very low in the 2 groups with no significant difference between them (x2 =0.347,P > 0.05).The 2 groups both showed infrequent and mild adverse reactions.Conclusion Combined glucocorticoids showed marked therapeutic effect and good safety for herpes zoster of the head and face in the elderly.

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Article in Chinese | WPRIM | ID: wpr-752061

ABSTRACT

Objective: To observe the effects of Zhige oral liquid on lipid metabolism in rats with alcoholic liver disease.Methods: Alcoholic liver disease rats were induced by alcoholic gavage plus normal diet. The intervention group was given different doses of Zhige oral liquid at the same time. The control group was given alcohol and oral administration of Jiejiuling oral liquid. After 12 weeks, the rats were sacrificed and serum ALT, AST, TC, TG and liver TC and TG levels were measured. Results: Compared with the normal group, the liver index, serum ALT, AST, TC, TG and liver TC and TG levels were significantly higher in the groups except the high dose group (P < 0.01 or 0.05), among which the model group and the low dose group had the most significant increase (P < 0.01) . Compared with the model group, the above indicators were decreased in the high, medium and low dose groups and the control group, and the decreasing trend was the same.That is, the high dose of the Zhige oral liquid was the most significant (P < 0.01), followed by the middle dose group and the control group (P < 0.05) . The decrease in the low dose group was the least significant. Compared with the control group, there were significant differences in the high-dose groups (P < 0.05) . Conclusion: A certain dose of Zhige oral liquid can inhibit or reduce the occurrence of alcoholic liver disease by improving lipid metabolism disorder.

20.
Article in Chinese | WPRIM | ID: wpr-735104

ABSTRACT

Objective To investigate the healing degree of lesions of herpes zoster in the head and face and the risk factor of the discosmetic sequelae.Methods 92 case of herpes zoster in the head and face were enrolled in this study.The patients were treated in dermatological clinic of Changshu No.1 People's Hospital from Jan.2017 to Jun.2018.The clinical data were recorded,such as the time of control,improving and healing of lesion,sex,age,position,the time of therapy starting and the behavior of scratching,extrusion and divest crust.The blood glucose and serum albumin of patients were measured.We observed the remained scars and performed statistic analysis.Results In diabetes group,the control and healing time was (2.40±1.28) d and (10.38+2.47) d respectively,meanwhile the control and healing time was (1.72 ± 0.94) d and (8.32 ± 2.66) d respectively in non-diabetes group (P<0.05).The single factor analysis indicated that the age,position,diabetes,lesion extrusion,divest crust were the correlation factors of the zoster scar in head and face (P<0.05).Nevertheless the multifactor logistic regression indicated that the position,diabetes and divest crust were independent risk factors of the zoster scar in head and face (P<0.05).Conclusions The healing of herpes zoster is affected by diabetes.The independent risk factors of zoster scar in head and face are non-trigeminal nerves positions,diabetes and divest crust.

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