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1.
Acta Pharmaceutica Sinica ; (12): 627-637, 2022.
Article in Chinese | WPRIM | ID: wpr-922877

ABSTRACT

Exosomes are a kind of endosomal vesicles that are secreted by most if not all living cells. Due to their capability of delivering a variety of cargos, such as tissue- or cell-specific proteins, lipids, and genetic materials, and their broad biological activities, exosomes have gained substantial attention as emerging therapeutics. Exosomes derived from mesenchymal stem cells (MSCs) and dendritic cells (DCs) are two types of exosomes that are widely studied. Many preclinical and clinical studies have shown that they have a satisfactory treatment effect in lung diseases, liver diseases, nervous system diseases, tumors, and other diseases. In addition, exosomes from macrophages, tumor cells, plant cells, and many other cells are getting more attention due to their therapeutic potential. Besides natural exosomes, research on engineered exosomes has also made plenty of progress. There have been several engineering methods of exosomes, such as targeting modification and loading of active ingredients. In this review, we summarize the research progress of therapeutic exosomes from different sources, and further discusses the application prospects of exosomes and possible challenges in the future.

2.
Article in English | WPRIM | ID: wpr-927641

ABSTRACT

OBJECTIVE@#To explore the association of single nucleotide polymorphisms (SNPs) of the vitamin D receptor gene ( VDR) with circulating lipids considering gender differences.@*METHODS@#Of the Han Chinese adults recruited from a health examination center for inclusion in the study, the circulating lipids, 25-hydroxyvitamin D (25OHD), and other parameters were measured. The VDR SNPs of Cdx2 (rs11568820), Fok1 (rs2228570), Apa1 (rs7975232), and Taq1 (rs731236) were genotyped with a qPCR test using blood DNA samples, and their associations with lipids were analyzed using logistic regression.@*RESULTS@#In the female participants ( n = 236 with dyslipidemia and 888 without dyslipidemia), multiple genotype models of Fok1 indicated a positive correlation of B (not A) alleles with LDLC level ( P < 0.05). In the male participants ( n = 299 with dyslipidemia and 564 without dyslipidemia), the recessive model of Cdx2 and the additive and recessive models of Fok1 differed ( P < 0.05) between the HDLC-classified subgroups, respectively, and Fok1 BB and Cdx2 TT presented interactions with 25OHD in the negative associations with HDLC ( P < 0.05).@*CONCLUSION@#In the Chinese Han adults included in the study, the Fok1 B-allele of VDR was associated with higher LDLC in females, and the Fok1 B-allele and the Cdx2 T-allele of VDR were associated with lower HDLC in males. The interaction of VD and Fok1 BB or Cdx2 TT in males synergistically decreased HDLC levels.


Subject(s)
Adult , Alleles , Asians/genetics , China/ethnology , Dyslipidemias/genetics , Female , Genetic Predisposition to Disease/genetics , Genotype , Humans , Lipids/blood , Male , Middle Aged , Polymorphism, Single Nucleotide , Receptors, Calcitriol/genetics , Sex Factors , Vitamin D/blood
3.
Rev. bras. med. esporte ; 27(5): 472-475, July-Sept. 2021. tab, graf
Article in English | LILACS | ID: biblio-1288614

ABSTRACT

ABSTRACT Introduction: Community sports activities are performed by people in the community environment, using beneficial factors such as sports equipment, facilities, and natural resources in the community to improve their body structure and functions, and their activity participation levels. Objective: The thesis studies how to use the framework and theory of "International Classification of Functions, Disability and Health" and World Health Organization "Community Rehabilitation Guidelines" to construct a sports service system to promote community health. Methods: The thesis uses the theory and methods related to function, disability, and health of the International Classification of Functions, Disability, and Health, and the matrix of the World Health Organization's Community Rehabilitation Guidelines as the framework for community sports activities and integrated development. Results: The development of community sports activity services can improve the physical function and athletic ability of different participants, promote healthy behaviors, improve mental health, and improve happiness. Community sports activities can promote the community's health, rehabilitation, education, career development, and empowerment. The integrated development of community sports activities and community health and rehabilitation activities will build a modern health service system. Conclusions: Use "International Classification of Function, Disability, and Health" to construct a community sports activity framework based on function orientation and overall health. Use the matrix and methods of the World Health Organization's "Community Rehabilitation Guidelines" to promote the integration of sports activities with activities such as health, rehabilitation, education, professional development, and empowerment to achieve inclusive community development and overall community health. Level of evidence II; Therapeutic studies - investigation of treatment results.


RESUMO Introdução: As atividades esportivas comunitárias são realizadas por pessoas do meio comunitário, utilizando fatores benéficos como equipes esportivas, instalações e recursos naturais da comunidade para melhorar sua estrutura e funções corporais e seus níveis de participação na atividade. Objetivo: A tese estuda como usar a estrutura e a teoria da "Classificação Internacional de Funções, Incapacidade e Saúde" e as "Diretrizes de Reabilitação Comunitária" da Organização Mundial de Saúde para construir um sistema de serviços esportivos para promover a saúde da comunidade. Métodos: A tese usa a teoria e métodos relacionados à função, deficiência e saúde da Classificação Internacional de Funções, Incapacidade e Saúde, e a matriz das Diretrizes de Reabilitação Comunitária da Organização Mundial de Saúde como uma estrutura para atividades esportivas comunitárias e desenvolvimento integrado. Resultados: O desenvolvimento de serviços de atividades esportivas comunitárias pode melhorar a função física e a capacidade atlética dos diferentes participantes, promover comportamentos saudáveis, melhorar a saúde mental e melhorar a felicidade. As atividades esportivas da comunidade podem promover saúde, reabilitação, educação, desenvolvimento profissional e empoderamento da comunidade. O desenvolvimento integrado de atividades esportivas comunitárias e atividades de saúde e reabilitação comunitárias criará um sistema moderno de serviços de saúde. Conclusões: Use a "Classificação Internacional de Função, Incapacidade e Saúde" para construir uma estrutura de atividade esportiva comunitária baseada na orientação funcional e saúde geral. Use a matriz e os métodos das "Diretrizes de Reabilitação da Comunidade" da Organização Mundial da Saúde para promover a integração das atividades esportivas com atividades como saúde, reabilitação, educação, desenvolvimento profissional e capacitação para alcançar o desenvolvimento comunitário inclusivo e saúde da comunidade em geral. Nível de evidência II; Estudos terapêuticos: investigação dos resultados do tratamento.


RESUMEN Introducción: Las actividades deportivas comunitarias son realizadas por personas del entorno comunitario, utilizando factores beneficiosos como equipos deportivos, instalaciones y recursos naturales de la comunidad para mejorar su estructura y funciones corporales, y sus niveles de participación en la actividad. Objetivo: La tesis estudia cómo utilizar el marco y la teoría de la "Clasificación internacional de funciones, discapacidad y salud" y las "Pautas de rehabilitación comunitaria" de la Organización Mundial de la Salud para construir un sistema de servicios deportivos para promover la salud comunitaria. Métodos: La tesis utiliza la teoría y los métodos relacionados con la función, la discapacidad y la salud de la Clasificación Internacional de Funciones, Discapacidad y Salud, y la matriz de las Pautas de rehabilitación comunitaria de la Organización Mundial de la Salud como marco para las actividades deportivas comunitarias y el desarrollo integrado. Resultados: El desarrollo de servicios comunitarios de actividades deportivas puede mejorar la función física y la capacidad atlética de los diferentes participantes, promover comportamientos saludables, mejorar la salud mental y mejorar la felicidad. Las actividades deportivas comunitarias pueden promover la salud, la rehabilitación, la educación, el desarrollo profesional y el empoderamiento de la comunidad. El desarrollo integrado de las actividades deportivas comunitarias y las actividades comunitarias de salud y rehabilitación construirá un moderno sistema de servicios de salud. Conclusiones: Utilice la "Clasificación internacional de función, discapacidad y salud" para construir un marco de actividad deportiva comunitaria basado en la orientación funcional y la salud en general. Utilice la matriz y los métodos de las "Pautas de rehabilitación comunitaria" de la Organización Mundial de la Salud para promover la integración de actividades deportivas con actividades como salud, rehabilitación, educación, desarrollo profesional y empoderamiento para lograr el desarrollo comunitario inclusivo y la salud comunitaria en general. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.


Subject(s)
Humans , Community Participation , Sports Equipment , Health Promotion
4.
Article in English | WPRIM | ID: wpr-890499

ABSTRACT

Background@#Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction, and its relationship with blood pressure (BP) has been controversial. The aim of the study was to analyze the association between SCH and newly-diagnosed hypertension. @*Methods@#Based on data from the Thyroid disease, Iodine nutrition and Diabetes Epidemiology (TIDE) study, 49,433 euthyroid individuals and 7,719 SCH patients aged ≥18 years were enrolled. Patients with a history of hypertension or thyroid disease were excluded. SCH was determined by manufacturer reference range. Overall hypertension and stage 1 and 2 hypertension were diagnosed according to the guidelines issued by the American College of Cardiology/American Heart Association in 2017. @*Results@#The prevalence of overall hypertension (48.7%), including stage 1 (28.9%) and 2 (19.8%) hypertension, increased significantly in SCH patients compared with euthyroid subjects. With elevated serum thyroid stimulating hormone (TSH) level, the hypertension prevalence also increased significantly from the euthyroid to different SCH subgroups, which was more profound in females or subjects aged <65 years. The age- and sex-specific regression analysis further demonstrated the same trends in the general population and in the 1:1 propensity matched population. Similarly, several BP components (i.e., systolic, diastolic, and mean arterial BP) were positively associated with TSH elevation, and regression analysis also confirmed that all BP components were closely related with SCH in female subjects aged <65 years. @*Conclusion@#The prevalence of hypertension increases for patients with SCH. SCH tends to be associated with hypertension and BP components in females younger than 65 years.

5.
Chinese Journal of School Health ; (12): 1000-1004, 2021.
Article in Chinese | WPRIM | ID: wpr-886310

ABSTRACT

Objective@#To explore the effect of long term aerobic exercise on microcirculation function and VO 2max of college students, and to explore the relationship between microcirculation function and VO 2max .@*Methods@#Seventy sports major college students were selected as the exercise group, 70 sedentary non sports major students were selected as the control group. PF6000 dual channel laser Doppler blood flow detector was used to assess the skin microcirculation function on the quadriceps femoris muscle of the subjects right lower limbs.The PFT Ergo cardiopulmonary function test system and the equipped power bicycle were used to measure the VO 2max of the subjects.@*Results@#The baseline value of MBP in the exercise group was significantly lower than that in the control group (6.5±1.8 vs. 8.1±2.5), but the microvascular reactivity(1 666.3±588.6 vs. 1 165.2±407.1) was significantly higher than that in the control group(t =-3.42,4.46, P <0.01). The absolute value of VO 2max (2 684.8±451.1 vs. 2 474.4±423.5) and relative value (42.7±4.3 vs. 35.1±4.8) in exercise group were significantly higher than those in control group( t =2.16,3.25, P < 0.01 ). Multiple regression showed that there was a positive correlation between microvascular reactivity and VO 2max (relative value) ( β = 0.40 , P =0.03), but no significant correlation between percutaneous oxygen partial pressure and VO 2max (relative value) ( β= 0.23 , P =0.19).@*Conclusion@#Long term physical exercise can decrease microcirculation perfusion volume, improve microvascular reactivity, percutaneous oxygen partial pressure among college students. Improvement of the microvascular reactivity may be a sport improving VO 2max (relative) one of the most important peripheral physiological mechanism.

6.
Article in Chinese | WPRIM | ID: wpr-906496

ABSTRACT

Objective:To explore the difference in antibacterial mechanism between <italic>Coptis chinensis</italic> and<italic> </italic>its<italic> </italic>flower stalk based on secondary metabolites and network pharmacology. Method:Based on the ultraperformance liquid chromatography mass spectrometry(UPLC-MS/MS) detection platform,the secondary metabolites database of <italic>C. chinensis</italic> and its flower stalk(MWDB) was built. The common database of metabolites information and the multivariate statistical analysis were used to study the differences of secondary metabolites between <italic>C. chinensis</italic> and its flower stalk and screen out 18 metabolites of<italic> </italic>the<italic> </italic>flower stalk and 11 metabolites of <italic>C. chinensis</italic> with a high content. BATMAN-TCM database was used to obtain the targets of component action,and their corresponding genes were inquired in the UniProt database. GeneCards was retrieved for antimicrobial genes,and the intersection genes of components and antimicrobials were obtained on Venny platform. Through DAVID gene ontology(GO) enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis,the mechanism of its action was predicted,and the results were visualized through histogram and advanced bubble diagram drawn by GraphPad Prism software and OmicShare database. The protein-protein interaction(PPI) network was constructed by STRING, database and the component-target-pathway network was constructed by Cytoscape 3.7.2 software. The antibacterial differences were compared based on the results of network pharmacology analysis. Result:Through network pharmacology,the antibacterial active components of <italic>C. chinensis</italic> were 5 fewer than that of the flower stalk,55 more antibacterial targets than that of the flower stalk; quercetin and berberine were predicted to be the common components of the antagonistic action of <italic>C. chinensis </italic>and the flower stalk. Key genes involved in antimicrobial action were p38 Mitogen-activated protein kinase 14(MAPK14),catalase(CAT); malaria and Toll-like receptor signaling pathway were different key pathways involved in antimicrobial activity. Conclusion:<italic>C. chinensis </italic>and the flower stalk mainly exert the antibacterial effect in a multi-target and multi-pathway manner,which can offer new ideas and clues for the study of antibacterial mechanism of<italic> C. chinensis</italic> and the flower stalk,and provide a new development direction for the comprehensive development and rational application of the flower stalk resources.

7.
Article in English | WPRIM | ID: wpr-898203

ABSTRACT

Background@#Subclinical hypothyroidism (SCH) is the most common thyroid dysfunction, and its relationship with blood pressure (BP) has been controversial. The aim of the study was to analyze the association between SCH and newly-diagnosed hypertension. @*Methods@#Based on data from the Thyroid disease, Iodine nutrition and Diabetes Epidemiology (TIDE) study, 49,433 euthyroid individuals and 7,719 SCH patients aged ≥18 years were enrolled. Patients with a history of hypertension or thyroid disease were excluded. SCH was determined by manufacturer reference range. Overall hypertension and stage 1 and 2 hypertension were diagnosed according to the guidelines issued by the American College of Cardiology/American Heart Association in 2017. @*Results@#The prevalence of overall hypertension (48.7%), including stage 1 (28.9%) and 2 (19.8%) hypertension, increased significantly in SCH patients compared with euthyroid subjects. With elevated serum thyroid stimulating hormone (TSH) level, the hypertension prevalence also increased significantly from the euthyroid to different SCH subgroups, which was more profound in females or subjects aged <65 years. The age- and sex-specific regression analysis further demonstrated the same trends in the general population and in the 1:1 propensity matched population. Similarly, several BP components (i.e., systolic, diastolic, and mean arterial BP) were positively associated with TSH elevation, and regression analysis also confirmed that all BP components were closely related with SCH in female subjects aged <65 years. @*Conclusion@#The prevalence of hypertension increases for patients with SCH. SCH tends to be associated with hypertension and BP components in females younger than 65 years.

8.
Article in Chinese | WPRIM | ID: wpr-781459

ABSTRACT

OBJECTIVE@#to explore the value of capillary electrophoresis in screening β- thalassemia of children, and to establish the cutoff values of HbA2 and HbF in our laboratory.@*METHODS@#The data of hemoglobin capillary electrophoresis and genetic diagnosis of β- thalassemia from 886 examined children were retrospectively analyzed. The cutoff values of HbA2 and HbF were determined by ROC curve.@*RESULTS@#The cutoff value of HbA2 screening minor β- thalassemia was 3.65%, the specificity was 0.996, and the sensitivity was 0.995. The cut-off value of HbF for screening minor β- thalassemia was 1.45%, specificity was 0.751 and sensitivity was 0.675. Thus, 1 case with codon5 (CCT→C) mutation, 1 case with SEA -HPFH β deletion, 1 case with - 28 (A→G) merger IVS-Ι-128 (T→G) double heterozygous mutations yet were found out, 1 case with 47 bp β gene missing has not yet been reported in literature.@*CONCLUSION@#Capillary electrophoresis has more high sensitivity and specificity in the screening of β- thalassemia in children, especially for the detection of rare β- thalassemia.

9.
Article in English | WPRIM | ID: wpr-827434

ABSTRACT

Acupuncture has been widely used for treating diseases since the ancient days in China, but the mechanism by which acupuncture exerts such powerful roles is unclear. Epigenetics, including DNA methylation, histone modification, and post-transcriptional regulation of miRNAs, is the study of heritable changes in gene expression that do not include DNA sequence alterations. Epigenetics has become a new strategy for the basic and clinical research of acupuncture in the last decade. Some investigators have been trying to illustrate the mechanism of acupuncture from an epigenetics perspective, which has shed new lights on the mechanisms and applications of acupuncture. Moreover, the introduction of epigenetics into the regulatory mechanism in acupuncture treatment has provided more objective and scientific support for acupuncture theories and brought new opportunities for the improvement of acupuncture studies. In this paper, we reviewed the literatures that has demonstrated that acupuncture could directly or indirectly affect epigenetics, in order to highlight the progress of acupuncture studies correlated to epigenetic regulations. We do have to disclose that the current evidence in this review is not enough to cover all the complex interactions between multiple epigenetic modifications and their regulations. However, the up-to-date results can help us to better understand acupuncture's clinical applications and laboratory research.

10.
Cancer Research and Clinic ; (6): 613-617, 2020.
Article in Chinese | WPRIM | ID: wpr-872555

ABSTRACT

Objective:To investigate the clinical significance of the serum platelet-derived growth factor BB (PDGF-BB) level in the diagnosis and treatment monitoring of non-small cell lung cancer (NSCLC) patients.Methods:Serum samples of 111 NSCLC patients in Xuzhou Cancer Hospital of Jiangsu Province and 105 healthy controls from Outpatients Department of Nanjing Yian Medical Examination Center between October 2014 and December 2017 were collected. Luminex assay was used to determine PDGF-BB level in serum samples of NSCLC patients and the healthy controls as well as patients after platinum chemotherapy. Receiver operating characteristic curve (ROC) was used to evaluate the diagnostic value of serum PDGF-BB level in patients with NSCLC, and the relationship of serum PDGF-BB level with clinicopathological features of NSCLC patients and platinum chemotherapy and effect was analyzed.Results:Compared to the healthy controls, serum PDGF-BB level was decreased in NSCLC patients [(54±24) ng/ml vs. (125±61) ng/ml, t = 11.09, P < 0.01], and the area under the ROC of serum PDGF-BB level on the diagnosis of NSCLC was 0.890. There were no significant differences in serum PDGF-BB level among NSCLC patients with different gender, age or lymph node metastasis (all P > 0.05); the serum PDGF-BB level in patients with high TNM stage was lower than that in patients with low TNM stage, and the serum PDGF-BB level in patients with low differentiation degree was lower than that in patients with high differentiation degree (all P < 0.05). Serum PDGF-BB level was continued to decrease in NSCLC patients after treatment with platinum-based chemotherapy. Conclusion:Serum PDGF-BB level of NSCLC patients is decreased, and it has a certain clinical significance in the early diagnosis of NSCLC.

11.
Article in Chinese | WPRIM | ID: wpr-793324

ABSTRACT

Diabetes mellitus patients are at high risk of tuberculosis, and tuberculosis is one of the important reasons for inducing and aggravating acute complications of diabetes. At present, China is facing a serious dual epidemic of tuberculosis and diabetes, which has caused great harm to the health of our people, but also brought great burden to our social economy. In this paper, PubMed, Web of Science, CNKI and Wanfang data knowledge service platform were used to search the relevant literature. Research progress on the epidemiology of tuberculosis patients complicated with diabetes, the effect of diabetes and blood glucose on the treatment outcome of tuberculosis patients, and the mechanism of diabetes affecting the treatment outcome of tuberculosis patients were analyzed and summarized, and some references were provided for the treatment and management of tuberculosis patients in China in the future.

12.
Article in Chinese | WPRIM | ID: wpr-849714

ABSTRACT

Objective To evaluate the relationship between the platelet reactivity detected by two methods and CYP2C19 gene polymorphism in patients with ACS after taking clopidogrel, and analyze the correlation between different platelet detection methods. Methods Forty-three patients with ACS, admitted in the General Hospital of Northern Theater Command from July to October 2019, met the diagnostic criteria and received dual antiplatelet therapy, were enrolled in present study. The gene chip was used for CYP2C19 genotype detection, vasodilatory stimulated phosphorylated protein (VASP) method and two revulsiveinduced phototurbidimetric methods [arachidonic acid-induced phototurbidimetry (AA-LTA), diphosphate adenosine-induced phototurbidimetry (ADP-LTA)] were used to evaluate the antiplatelet reactivity, and the correlation between different evaluation methods was compared. Results Of the 43 patients, 17(39.5%) were EMs, 19(44.2%) were IMs, and 7(16.3%) were PMs. The results of VASP were higher in PMs and IMs patients than those in EMs, and the results of AA-LTA were higher in PMs than those in IMs and EMs. The differences were significant (P<0.01). The platelet reactivity of VASP was positively correlated with AA-LTA and ADP-LTA (r=0.383, 0.369, P<0.05); and AA-LTA was positively correlated with ADP-LTA (r=0.496, P=0.011). Conclusions There is a difference between CYP2C19 gene polymorphism and platelet reactivity, and the correlation between VASP method and LTA method is low. CYP2C19 genotype can be used as a reference for the platelet reactivity of patients after clopidogrel therapy.

13.
Article in Chinese | WPRIM | ID: wpr-826670

ABSTRACT

OBJECTIVE@#To compare the therapeutic effect of - balance penetrating acupuncture combined with rehabilitation training and single rehabilitation training on upper limb spasticity in patients with stroke hemiplegia.@*METHODS@#A total of 60 patients with upper limb spasticity of stroke hemiplegia were randomized into an observation group and a control group, 30 cases in each one. On the basis of conventional western medication, rehabilitation training was adopted in the control group. On the basis of treatment in the control group, - balance penetrating acupuncture was applied from Jianyu (LI 15) to Binao (LI 14), Quchi (LI 11) to Shaohai (HT 3), Yanglingquan (GB 34) to Yinlingquan (SP 9), Xuanzhong (GB 39) to Sanyinjiao (SP 6), etc. of the affected side in the observation group. The treatment was given once a day, 5 days were as one course, with a 2-day interval between two courses, 4 courses were required in both groups. The classification of modified Ashworth spasticity scale (MAS), surface integrated electromyogram (iEMG) of affected upper limb and the scores of National Institute of Health stroke scale (NIHSS), Fugl-Meyer assessment (FMA) of upper limb and modified Barthel index (MBI) before and after treatment were observed, the therapeutic effect was evaluated in both groups.@*RESULTS@#①After treatment, the MAS classification reduced in both groups (<0.05), the cases of grade 0 to Ⅰ in the observation group were more than those in the control group (<0.05); iEMG values of the maximum isometric voluntary contraction of affected usculus biceps brachii, musculus triceps brachii, musculus flexor carpi, musculus extensor carpi, extensor digitorum, aductor pollicis brevis were increased in both groups (<0.05), and the variations of iEMG of above muscles on the affected side in the observation group were larger than those in the control group (<0.05). ②After treatment, the scores of NIHSS were decreased (<0.05), the scores of FMA, MBI were increased in both groups (<0.05), and the variations of NIHSS, FMA and MBI scores were larger than those in the control group (<0.05). ③The total effective rate was 93.3% (28/30) in the observation group, which was superior to 70.0% (21/30) in the control group (<0.05).@*CONCLUSION@#- balance penetrating acupuncture combined with rehabilitation training can improve upper limb spasticity, heighten the motor function of upper limb and daily self care in patients with stroke hemiplegia, its therapeutic effect is superior to single rehabilitation training.


Subject(s)
Acupuncture Therapy , Hemiplegia , Therapeutics , Humans , Stroke , Therapeutics , Stroke Rehabilitation , Treatment Outcome , Upper Extremity , Yin-Yang
14.
Journal of Medical Postgraduates ; (12): 850-854, 2020.
Article in Chinese | WPRIM | ID: wpr-823281

ABSTRACT

ObjectiveWearing protective masks for a long time causes a large number of frontline health care workers to suffer different degrees of pressure injury or facial skin rupture in response to COVID-19. This paper aims to analyze the occurrence characteristics and related factors of pressure injury related to devices, and provide the basis for taking countermeasures. Methods There was online investigation of skin injuries caused by wearing protective equipment in medical staff. Descriptive analysis was carried out on the occurrence characteristics of pressure-induced injury, and influencing factors were analyzed through logistic regression model. ResultsThere were a total of 2901 valid questionnaires. The results showed that the incidence of pressure injury caused by protective equipment was 26.34%, mainly in the bridge of the nose (20.41%), cheek (20.23%), auricle (17.82%) and forehead (8.86%). Multivariate Logistic regression model analysis showed that the major associated factors, which presented increasing risk, were sweating and dampness (OR=12.72, 95%CI 8.36-17.30), wearing level-3 protective equipment (OR=3.55, 95%CI 2.47-5.08), wearing level-2 protective equipment (OR=3.37, 95%CI 2.47-4.60), wearing time (OR=1.29, 95%CI 1.05~1.58) and occupation (OR=1.57, 95%CI 1.00~2.49). Conclusion There is the high incidence of pressure injuries to health care workers caused by protective equipment against COVID-19. The main risk factors for facial stress injury of medical staff are sweating and dampness, wearing level-3 and level-2 protective equipment and wearing time.

15.
Chinese Journal of School Health ; (12): 1047-1051, 2020.
Article in Chinese | WPRIM | ID: wpr-823176

ABSTRACT

Objective@#To investigate psychosexual health of students during pubertal transition in Taizhou and explore the influence of family factors.@*Methods@#A questionnaire survey was conducted among 1 215 students in senior grades of primary school, junior and senior high schools in Taizhou by stratified cluster sampling.@*Results@#The average score of adolescents sexual and mental health questionnaire was (3.82±0.52), the sexual knowledge score was (3.31±1.00) and the score of sexual adaptation was (4.29±0.68). Sexual attitude and sexual control among female students were significantly better than male students (t=4.53,6.96,P<0.01). The four dimensions of physiological knowledge, sexual knowledge, sexual concept and self-adaptation made significant progress with the improvement increased with educational background(F=157.18,85.18,85.96,65.54,P<0.01). The parent-child relationship, parental sex education, the only child in the family, education background of parents, family economic status and the long-term separation from parents in early childhood had predictive effects on the development of puberty students’ psychosexual health (P<0.05).@*Conclusion@#Students during pubertal transition in Taizhou show moderate level of psychosexual health. Schools should strengthen sexual knowledge education.In order to promote students’ psychosexual health, more attention should be paid to the influence of family factors.

16.
Acta Physiologica Sinica ; (6): 637-644, 2019.
Article in Chinese | WPRIM | ID: wpr-777147

ABSTRACT

Mixed linked leukemia 4 (MLL4) is a specific methyltransferase of histone 3 position lysine 4 (H3K4). It is also one of the important members of COMPASS/Set1-like protein complex. Both MLL4 protein itself and its mediated H3K4 methylation modification can cause changes in chromatin structure and function, thus regulating gene transcription and expression. With the studies of MLL4 protein in recent years, the roles of MLL4 gene, MLL4 protein and protein complex in the development of tissues and organs, tumor diseases and other physiological and pathophysiological processes have been gradually revealed. In this paper, the research progress of MLL4 gene, MLL4 protein characteristics, biological function and its effect on disease were reviewed, in order to further understand the effect of histone methyltransferase on gene expression regulation, as well as its non-enzyme dependent function. This paper may provide new ideas for the prevention, diagnosis and treatment of related diseases.


Subject(s)
DNA-Binding Proteins , Physiology , Histone-Lysine N-Methyltransferase , Physiology , Histones , Chemistry , Humans , Methylation
17.
Article in English | WPRIM | ID: wpr-776704

ABSTRACT

There are differences in individual cardiovascular responses to the administration of dexmedetomidine, a highly selective α-adrenergic receptor (ADRA2A) agonist. The aim of this study was to investigate ADRA2A gene polymorphisms in the Chinese Han population and their association with the cardiovascular response to intravenous dexmedetomidine infusion. Sixty elective surgery patients of Chinese Han nationality were administered 1 µg/kg dexmedetomidine intravenously over 10 min as a premedication. ADRA2A C-1291G and A1780G polymorphism status was determined in these patients, and their relationships to changes in blood pressure and heart rate after dexmedetomidine administration were analyzed. There were neither significant differences in systolic or diastolic blood pressure changes in individuals with different A1780G and C-1291G genotypes after dexmedetomidine administration, nor in heart rates among the different A1780G genotypes. However, there were significant differences in changes in heart rates in patients with different C-1291G genotypes. There were no significant differences in the sedative effects of dexmedetomidine among different A1780G and C-1291G genotypes. Logistic regression revealed that the C-1291G polymorphism was associated with differential decreases in heart rate after intravenous infusion of dexmedetomidine. These findings indicate that the ADRA2A C-1291G polymorphism can affect heart rate changes in patients after intravenous infusion of dexmedetomidine.

18.
Article in Chinese | WPRIM | ID: wpr-817884

ABSTRACT

Balloon dilatation under bronchoscope is mainly used in the treatment of benign airway stenosis in children. It is a safe,simple and minimally invasive treatment. It also plays an important role in the removal of some special foreign bodies and the treatment for massive hemoptysis. Interventional therapy under bronchoscope is a comprehensive treatment technique,and balloon dilatation needs to be combined with other methods.

19.
Article in Chinese | WPRIM | ID: wpr-744235

ABSTRACT

AIM:To investigate the effect of salvianolic acid B (Sal B) on high glucose-induced phenotypic transition and extracellular matrix (ECM) secretion in human glomerular mesangial cells (HGMCs) and the underlying mechanisms.METHODS:HGMCs were randomly divided into control group, high glucose group and high glucose plus high dose, medium dose and low dose of Sal B groups.The HGMCs except those in control group were exposed to high glucose (33.3 mmol/L) for 72 h, while those in Sal B groups were co-incubated with indicated concentrations of Sal B.The protein levels ofα-smooth muscle actin (α-SMA) , transforming growth () and phosphorylated Smad2 and p38 mitogen-activated protein kinase (MAPK) were determined by Western blot.The secretion levels of collagen type I (Col I) , collagen type III (Col III) , fibronectin (FN) and laminin (LN) were measured by ELISA.RESULTS:Exposure to high glucose markedly increased the protein expression ofI, Col III, FN and LN in the HG-MCs (P<0.01).The phosphorylation levels of Smad2 and p38 MAPK were also significantly increased (P<0.01).Coincubation with Sal B evidently decreased the protein expression ofI, Col III, FN and LN in the HGMCs induced by high glucose (P<0.05 or P<0.01).The phosphorylated levels of Smad2 and p38 MAPK were also reduced noticeably (P<0.05 or P<0.01).CONCLUSION:Sal B significantly suppresses high glucose-induced phenotypic transition and ECM secretion in the HGMCs, which might be attributed, at least partly, to inhibition ofSmad signaling pathway and p38 MAPK activation.

20.
Acupuncture Research ; (6): 854-857, 2019.
Article in Chinese | WPRIM | ID: wpr-844239

ABSTRACT

Bloodletting puncture at twelve well-points is a characteristic emergency therapy in traditional Chinese medicine. This article reviewed the research advances in the clinical effect of this therapy in the treatment of acute central nervous injury and its mechanism of action over the past 30 years, and it is found that this therapy can effectively improve disturbance of consciousness, neurological defects, and cerebral edema caused by stroke, traumatic brain injury, and carbon monoxide poisoning. The mechanism involves the improvement of cerebral blood flow and tissue oxygen supply, repair of the blood-brain barrier, and regulation of local ion balance. Well-designed clinical trials and in-depth research on biological mechanisms should be performed in future to promote and guide its clinical application.

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