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Article in Chinese | WPRIM | ID: wpr-324834


<p><b>OBJECTIVE</b>To develop methods for qualitative and quantitative analyses of Flos Cartnami from three aspects, pigments, flavonoids and adenosine.</p><p><b>METHOD</b>A method using HPLC coupled with electrochemical detector was developed to determine the content of hydroxysafflor yellow A and fingerprint of Flos Carthami. The chromatographic separation was performed on a Zorbax SB C18 column (4.6 mm x 250 mm, 5 microm) by gradient elution with phosphate buffer and acetonitrile at a flow-rate of 1.0 mL x min(-1), the column temperature was 35 degrees C, the reference electrode was ISAAC (in-situ silver/silver chloride), the working electrode was glassy carbon, the counter electrode was Pt, and the applied potential was + 800 mV. Concentration of adenosine was determined by HPLC-UV on an Diamonsil C18 column (4.6 mm x 250 mm, 5 microm) with water-acetonitrile (95:5) as mobile phase, the flow rate was 1.0 mL x min(-1), the column temperature was 40 degrees C and the detection wavelength was 260 nm. The content of cartharmin was detected using a spectrophotometric method.</p><p><b>RESULT</b>Twenty-one common chromatographic peaks were selected as characteristic peaks in the chromatogram of sample solution of Flos Cartnami. Seven peaks were identified as hydroxysafflor yellow A, 6-hydroxykaempferol-3-O-glucoside, rutin, quercetin-3-O-glucoside, kaempferol-3-O-rutinoside, quercetin, kaempferol. The contents of hydroxysafflor yellow A and adenosine were from 0.35% to 3.58% and from 0.03% per hundred to 0.49% per hundred, respectively.</p><p><b>CONCLUSION</b>The methods can be used to evaluate the quality of Flos Carthami.</p>

Adenosine , Chemistry , Chalcone , Chemistry , Chromatography, High Pressure Liquid , Flavonoids , Chemistry , Plants, Medicinal , Chemistry , Quinones , Chemistry
Article in Chinese | WPRIM | ID: wpr-324284


<p><b>OBJECTIVE</b>To establish a method for determining the content of primary chemical constituents in the leaves of Cassia angustifolia.</p><p><b>METHOD</b>The HPLC with Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used , acetonitrile-1% acetic acid (10:90-15: 85-18: 82-20: 80-25: 75) in a gradient manner was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 40 degrees C and detection wavelength at 270 nm.</p><p><b>RESULT</b>The results showed that 5 effective components all separated well and showed good linearity.</p><p><b>CONCLUSION</b>The method was proved to be rapid, sensitive, accurate, credible and repeatable. It can be applied to quality control of Folium Sennae.</p>

Anthraquinones , Chemistry , Apigenin , Chemistry , Chromatography, High Pressure Liquid , Methods , Glucosides , Chemistry , Naphthalenes , Chemistry , Reproducibility of Results , Senna Extract , Senna Plant , Chemistry , Temperature
Article in Chinese | WPRIM | ID: wpr-264837


<p><b>OBJECTIVE</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The constituents were separated and purified by column chromatography with silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as demethoxymatteucinol (1), matteucinol (2), pinosylvin (3), matteuorien (4), pinosylvin 3-O-beta-D-glucopyranoside (5), matteuorienate A (6).</p><p><b>CONCLUSION</b>All Compounds were isolated from this plant for the first time.</p>

Chromones , Chemistry , Dryopteridaceae , Chemistry , Flavonoids , Chemistry , Glucosides , Chemistry , Magnetic Resonance Spectroscopy , Rhizome , Chemistry , Stilbenes , Chemistry
Article in Chinese | WPRIM | ID: wpr-351838


<p><b>OBJECTIVE</b>To study the hepatotoxicity effects in rats with different extract of Fructus Gardeniae.</p><p><b>METHOD</b>Observe the change of appearance, behavior and weight of rats through oral gavage daily for 3 d. Weigh the liver and calculate the liver index. Detect the ALT, AST and TBIL. Observe the liver tissue by optical microscope.</p><p><b>RESULT</b>The weight and index of liver were increased by 3.08 g x kg(-1) aqueous extract, 1.62 g x kg(-1) alcoholic extract and 0.28 g x kg(-1) geniposide, compared to those of the blank group (P < 0.005, P < 0.001) and the activities of ALT, AST and the content of TBIL were also increased, compared to those of the blank group (P < 0.05, P < 0.001). The liver cells were obviously swell, necrotic and changed with inflammatory infiltrate.</p><p><b>CONCLUSION</b>Aqueous extract, alcoholic extract and geniposide displayed hepatotoxicity, and the geniposide which was the main substance of the Fructus Gardeniae might be mainly responsible for the hepatotoxicity.</p>

Alanine Transaminase , Blood , Animals , Aspartate Aminotransferases , Blood , Bilirubin , Blood , Drugs, Chinese Herbal , Toxicity , Female , Fruit , Chemistry , Gardenia , Chemistry , Iridoids , Toxicity , Liver , Pathology , Male , Organ Size , Plants, Medicinal , Chemistry , Pyrans , Toxicity , Random Allocation , Rats , Rats, Sprague-Dawley
Article in Chinese | WPRIM | ID: wpr-272769


<p><b>OBJECTIVE</b>To establish the method of determining the quantity of hypericin in Hypericum perforatum and determine the quantity of the hypericin in defferent medicinal materials and asepsis seedings which grow in defferent environment.</p><p><b>METHOD</b>The specimen is extracted with methanol--Pyridine (9:1) ultrasound extraction. Chromatographic assay is performed on a hypersily ODS2 (4.6 mm x 150 mm, 5 microm) column. The mobile phase is composed of methanol -1.56% dihydric natrium phosphate hydrogen natrium solution (shift solution's acidity to 2.1 with phosphoric acid)--ethyl acetate (4:1.9:1), velocity of flow is 1 mL x min(-1); column temperature is 35 degrees C; the detection wavelength is 590 nm.</p><p><b>RESULT</b>A satisfactory seperaration between hypericin and impurity. The calibration curve is linear over the range of 0.0524-0.2620 microg for hypericin (r = 0.9998). The average recovery of hypericin is 97.50%.</p><p><b>CONCLUSION</b>The quantity of hypericin in Hypericum perforatum has something to do with the genetic factor, environment factor, growing period and dry means. The method of determining the quantity of hypericin can be regarded as the method of controling the quantity of medicinal materials.</p>

China , Chromatography, High Pressure Liquid , Methods , Ecosystem , Hypericum , Chemistry , Genetics , Perylene , Plants, Medicinal , Chemistry , Genetics , Quality Control , Seasons