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1.
Braz. j. biol ; 82: e235508, 2022. graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1285600

Subject(s)
Animals , Ants , Pollination , Forests , Flowers
2.
Braz. j. biol ; 82: e233550, 2022. tab, graf
Article in English | MEDLINE, LILACS, VETINDEX | ID: biblio-1278499

Subject(s)
Phaseolus , Seeds
3.
Braz. j. biol ; 82: e233567, 2022. tab, graf
Article in English | LILACS | ID: biblio-1249220

ABSTRACT

Abstract This research was carried out aiming at evaluating the effects of nitrate and ammonium ions on nutrient accumulation, biochemical components and yield of Italian zucchini (cv. Caserta) grown in a hydroponic system under salt stress conditions. The experiment was carried out in a greenhouse utilizing an experimental design in randomized blocks, arranged in a 2 x 5 factorial scheme, with 4 replications. The treatments consisted of two forms of nitrogen (nitrate - NO3- and ammonium - NH4+) and 5 electrical conductivity levels of irrigation water (ECw) (0.5, 2.0, 3.5, 5.0 and 6.5 dS m-1). The analysis of the results indicated that supply of N exclusively in NH4+ form promotes greater damage to the leaf membrane and reduction in accumulation of macronutrients and higher Na+/K+, Na+/Ca++ and Na+/Mg++ ratios in the shoots of zucchini plants. Electrical conductivity of irrigation water above 2.0 dS m-1 reduces the accumulation of nutrients in shoot and yield of Italian zucchini plant. The toxicity of NH4+ under Italian zucchini plants overlap the toxicity of the salinity, since its fertilization exclusively with this form of nitrogen inhibits its production, being the NO3- form the most suitable for the cultivation of the species.


Resumo Este trabalho foi desenvolvido com o objetivo de avaliar os efeitos dos íons nitrato e amônio sobre o acúmulo de nutrientes e produção da abobrinha italiana (cv. Caserta) cultivada em sistema hidropônico sob estrese salino. O experimento foi conduzido em casa de vegetação utilizando o delineamento experimental em blocos casualizados, arranjados em esquema fatorial 2 x 5, com 4 repetições. Os tratamentos foram constituídos de duas formas de nitrogênio (nitrato - NO3- e amônio - NH4+) e cinco níveis de condutividade elétrica da água de irrigação (CEa) (0,5; 2,0; 3,5; 5,0 e 6,5 dS m-1). As análises dos resultados indicaram que suprimento de N exclusivamente em forma de NH4+ promove maiores danos na membrana foliar e redução no acúmulo de macronutrientes e maiores relações Na+/K+, Na+/Ca++ e Na+/Mg++ na parte aérea das plantas de abobrinha. A irrigação com água a cima de 2,0 dS m-1 reduz o acúmulo de nutrientes na parte aérea das plantas e a produção de abobrinha. A toxicidade do NH4+ sob abobrinha italiana sobrepõe-se à toxicidade da salinidade, pois a fertilização exclusiva com esta forma de nitrogênio inibe sua produção, sendo a forma NO3- a mais adequada para o cultivo da espécie.


Subject(s)
Ammonium Compounds , Nitrogen , Plant Roots/chemistry , Salt Stress , Homeostasis , Italy , Nitrates
4.
Braz. j. med. biol. res ; 54(6): e10754, 2021. tab, graf
Article in English | LILACS | ID: biblio-1285670

ABSTRACT

Epidermal growth factor receptor (EGFR) signaling and components of the fibrinolytic system, including urokinase-type plasminogen activator (uPA) and thrombomodulin (TM), have been implicated in tumor progression. In the present study, we employed cBioPortal platform (http://www.cbioportal.org/), cancer cell lines, and an in vivo model of immunocompromised mice to evaluate a possible cooperation between EGFR signaling, uPA, and TM expression/function in the context of cervical cancer. cBioPortal analysis revealed that EGFR, uPA, and TM are positively correlated in tumor samples of cervical cancer patients, showing a negative prognostic impact. Aggressive human cervical cancer cells (CASKI) presented higher gene expression levels of EGFR, uPA, and TM compared to its less aggressive counterpart (C-33A cells). EGFR induces uPA expression in CASKI cells through both PI3K-Akt and MEK1/2-ERK1/2 downstream effectors, whereas TM expression induced by EGFR was dependent on PI3K/Akt signaling alone. uPA induced cell-morphology modifications and cell migration in an EGFR-dependent and -independent manner, respectively. Finally, treatment with cetuximab reduced in vivo CASKI xenografted-tumor growth in nude mice, and decreased intratumoral uPA expression, while TM expression was unaltered. In conclusion, we showed that EGFR signaling regulated expression of the fibrinolytic system component uPA in both in vitro and in vivo settings, while uPA also participated in cell-morphology modifications and migration in a human cervical cancer model.


Subject(s)
Humans , Animals , Female , Rats , Uterine Cervical Neoplasms/drug therapy , Phosphatidylinositol 3-Kinases , Prognosis , Cell Movement , Cell Line, Tumor , ErbB Receptors , Mice, Nude
5.
Braz. j. biol ; 80(4): 798-802, Oct.-Dec. 2020. tab, graf
Article in English | LILACS | ID: biblio-1142516

ABSTRACT

Abstract Nasal mites are commonly found in hummingbirds (Apodiformes). In most cases, endoparasitic mites were reported to parasitize the respiratory system, particularly the anterior nasal chambers, larynx, trachea, lungs, and the air and conjunctival sacs. However, some mites are only carried by hummingbirds, because flowers serving as a nutrient source for these small mites, such as for Apodiformes, a tropical nectar-feeding in their natural habitat. From August 2012 to May 2014, humminbirds were captured using mist nets, and examined for the presence of nasal mites (identified according to specific keys) in the Pantanal, and Cerrado biomes, from the Mato Grosso state, midwestern Brazil. Overall, 76 Apodiform specimens, from the Trochilidae family were captured, and 20 female nasal mites, identified as being from the Rhinoseius genus, were collected from eight hosts (10.5%), of the following species: Amazilia fimbriata, Amazilia versicolor, Eupetonema macroura, and Thalurania furcata. The Rhinoseius spp. has been reported on, in many ecosystems in the Americas, including Brazil, however, this is the first report on Apodiformes from the Pantanal biome. Phoretic mites are not responsible for direct damage to the population of Apodiformes, as they are not pathogenic. However, they compete for food with hummingbirds and decrease the quantity of food available in the environment. They also affect the reproductive dynamics of plants, feed on pollen, and interfere with the intricacies of the biodiversity where they live.


Resumo Os ácaros nasais são comumente encontrados em beija-flores (Apodiformes). Na maioria dos casos, os ácaros foram relatados como endoparasitas, parasitando o sistema respiratório, particularmente as câmaras nasais anteriores, a laringe, a traqueia, os pulmões e os sacos aéreos e conjuntivais. No entanto, alguns ácaros são transportados apenas por beija-flores, pois flores servem como fonte de nutrientes para esses pequenos ácaros, assim como para Apodiformes tropicais alimentados de néctar em seu habitat natural. De agosto de 2012 a maio de 2014, beija-flores foram capturadas com redes de neblina e examinadas quanto à presença de ácaros nasais (identificados de acordo com chaves específicas) nos biomas Pantanal e Cerrado, no estado do Mato Grosso, Centro-Oeste do Brasil. No total, 76 exemplares Apodiformes da família Trochilidae foram capturados e 20 fêmeas de ácaros nasais, identificados como pertencentes ao gênero Rhinoseius, foram coletados de oito hospedeiros (10,5%), das seguintes espécies: Amazilia fimbriata, Amazilia versicolor, Eupetonema macroura e Thalurania furcata. Rhinoseius spp. foi relatado em muitos ecossistemas nas Américas, incluindo o Brasil, no entanto, este é o primeiro relato em Apodiformes do bioma Pantanal. Os ácaros foréticos não são responsáveis ​​por danos diretos à população de Apodiformes, pois não são patogênicos. No entanto, eles competem por comida com beija-flores e diminuem a quantidade de comida disponível no ambiente. Eles também afetam a dinâmica reprodutiva das plantas, se alimentam de pólen e interferem nas interações da biodiversidade em que vivem.


Subject(s)
Animals , Female , Mites , Birds , Brazil , Ecosystem , Plant Nectar
6.
Arq. bras. med. vet. zootec. (Online) ; 71(2): 545-552, mar.-abr. 2019. ilus
Article in English | LILACS, VETINDEX | ID: biblio-1011259

ABSTRACT

Were used twelve (12) adult anteaters (Myrmecophaga tridactyla), adults, 6 (six) males and 6 (six) females, weighing from 20 to 27.32kg from free life. The thoracic cavity was opened until visualization of the whole heart and lungs and later injection of the coronary vessels. The right coronary artery emerged through a single coronary ostium of the aorta, 50%, emitting the intermediate branch and the subsinuous interventricular branch, had a path directed to the subsurface interventricular groove. In the other 50%, the right coronary artery was not present, showing only its branches, intermediate branch and subsurface interventricular branch with emergence of the aorta. Left coronary artery presented, in 83.33%, origin from the aorta in single ostium, issuing the circumflex and interventricular paraconal branches. In 16.66%, the left coronary artery was not evidenced originating from the aorta, but its branches, circumflex and interventricular paraconal.(AU)


Foram utilizados 12 tamanduás-bandeira (Myrmecophaga tridactyla), adultos, sendo seis machos e seis fêmeas, pesando de 20 a 27,32kg, provenientes de vida livre. Foi realizada abertura da cavidade torácica até a visualização do conjunto coração e pulmões e, posteriormente, injeção dos vasos coronários. A artéria coronária direita emergia, 50% dela, por um único óstio coronário da aorta, emitindo o ramo intermédio e o ramo interventricular subsinuoso; tinha trajeto direcionado ao sulco interventricular subsinuoso. Nos outros 50%, a artéria coronária direita não estava presente, exibindo somente seus ramos, ramo intermédio e ramo interventricular subsinuoso com emergência da aorta. A artéria coronária esquerda apresentou, em 83,33%, origem a partir da aorta em óstio único, emitindo os ramos circunflexo e interventricular paraconal. Em 16,66%, a artéria coronária esquerda não foi evidenciada originando-se da aorta, mas, sim, de seus ramos, circunflexo e interventricular paraconal.(AU)


Subject(s)
Animals , Coronary Vessels/anatomy & histology , Cingulata/anatomy & histology , Heart
7.
Arq. bras. med. vet. zootec. (Online) ; 70(6): 1925-1934, nov.-dez. 2018. mapas, tab, ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-970670

ABSTRACT

O objetivo da realização deste trabalho foi analisar a variabilidade espacial da composição do leite cru refrigerado e elaborar mapas com interpolação de dados sobre os teores de gordura, proteína, lactose, sólidos totais e extrato seco desengordurado, no estado de Alagoas e na mesorregião do Agreste Pernambucano, em 2014 e 2015. Foram analisados 3.863 laudos oficiais de amostras de leite cru refrigerado, coletados de 432 tanques de expansão direta da região estudada. O grau de dependência espacial e a regressão geograficamente ponderada das variáveis foram analisados pelo software ArcGIS 10.3. A análise espacial mostrou predominância de áreas com teor de gordura de 3,1 a 3,6g/100g e áreas com teor de gordura de 3,6 a 4,2g/100g. Para o teor de lactose, foi observada área predominante com 4,32 a 4,45g/100g e algumas áreas com 4,46 a 4,54g/100g. Foi observada baixa influência da altitude, precipitação pluviométrica e interação precipitação x altitude sobre o teor de gordura, proteína, lactose, sólidos totais e extrato seco desengordurado na área estudada. Por fim, conclui-se que há variabilidade espacial para gordura, lactose, proteína, sólidos totais e extrato seco desengordurado do leite cru refrigerado produzido no estado de Alagoas e na mesorregião do Agreste Pernambucano.(AU)


The aim of this work was to analyze the spatial variability and draw maps with data interpolation on the fat, protein, lactose, total solids, and nonfat dry extract of refrigerated raw milk in the state of Alagoas and Mesoregion the Pernambuco Agreste in 2014 and 2015. A total of 3,863 fficial reports of samples of raw milk collected from 432 refrigerated tanks direct expansion of the studied region were analyzed. The degree of spatial dependence and geographically weighted regression of variables was analyzed using ArcGIS 10.3 software. The spatial analysis showed predominance of areas with a fat content of 3.1 to 3.6g/100g and areas with a fat content of 3.6 to 4.2g/100g. For lactose content predominant area of 4.32 to 4,45g/100g and some areas with 4.46 to 4,54g/100g were observed. Altitude, rainfall, and precipitation interaction x altitude of the fat, protein, lactose, total solids and nonfat dry extract in the study area showed little influence. Finally, there is spatial variability in fat, lactose, protein, total solids, and nonfat dry extract of refrigerated raw milk produced in the state of Alagoas and Pernambuco Mesoregion of Agreste.


Subject(s)
Milk/chemistry , Cooled Foods , Climatic Zones
8.
Braz. j. med. biol. res ; 51(1): e6822, 2018. tab, graf
Article in English | LILACS | ID: biblio-889012

ABSTRACT

Cervical cancer is a public health problem and the molecular mechanisms underlying radioresistance are still poorly understood. Here, we evaluated the modulation of key molecules involved in cell proliferation, cell cycle and DNA repair in cervical cancer cell lines (CASKI and C33A) and in malignant tissues biopsied from 10 patients before and after radiotherapy. The expression patterns of epidermal growth factor receptor (EGFR), excision repair cross-complementation group 1 (ERCC1) and p53 were evaluated in cancer cell lines by quantitative PCR and western blotting, and in human malignant tissues by immunohistochemistry. The mutation status of TP53 gene was evaluated by direct sequencing. Among cell lines, absent or weak modulations of EGFR, ERCC1 and p53 were observed after exposure to 1.8 Gy. Conversely, increased expressions of p53 (5/10 patients; P=0.0239), ERCC1 (5/10 patients; P=0.0294) and EGFR (4/10 patients; P=0.1773) were observed in malignant tissues after radiotherapy with the same radiation dose. TP53 mutations were found only in one patient. Here we show that a single dose of radiotherapy induced EGFR, ERCC1 and p53 expression in malignant tissues from cervical cancer patients but not in cancer cell lines, highlighting the gap between in vitro and in vivo experimental models. Studies on larger patient cohorts are needed to allow an interpretation that an upregulation of p53, EGFR and ERCC1 may be part of a radioresistance mechanism.


Subject(s)
Humans , Female , Adult , Middle Aged , Aged , Carcinoma, Squamous Cell/radiotherapy , Uterine Cervical Neoplasms/radiotherapy , Genes, p53/radiation effects , Genes, erbB-1/radiation effects , DNA-Binding Proteins/radiation effects , Endonucleases/radiation effects , Immunohistochemistry , Carcinoma, Squamous Cell/genetics , Carcinoma, Squamous Cell/pathology , Tumor Stem Cell Assay , Blotting, Western , Prospective Studies , Cell Line, Tumor , Mutation
9.
Braz. j. biol ; 77(2): 318-322, Apr.-June 2017. graf
Article in English | LILACS | ID: biblio-888750

ABSTRACT

Abstract Pleurolophocercous cercariae found in the invasive gastropod Melanoides tuberculata (Müller, 1774) collected in a stream of the Vila do Abraão, Ilha Grande, Angra dos Reis, Rio de Janeiro, Brazil were used for experimental infection that enabled the identification of the heterophyid trematode Centrocestus formosanus (Nishigori, 1924). The parasite has been found in the locality since 2007, after two years of the introduction of M. tuberculata. Recently, from a sample of 483 specimens collected in June 2013, 101 (21%) were found infected with parasite. The potential environmental impacts caused by the parasite occurrence could be underestimated in the country, and actions to monitor and control both the parasite and the mollusk are necessary.


Resumo Cercárias do tipo pleurolofocerca encontradas no gastrópode invasor Melanoides tuberculata (Müller, 1774) coletados em um riacho da Vila do Abraão em Ilha Grande, Angra dos Reis, Rio de Janeiro, Brasil foram utilizadas em estudos de infecção experimental que possibilitaram a identificação do trematódeo heterofiídeo Centrocestus formosanus (Nishigori, 1924). O parasito vem sendo encontrado na localidade desde 2007, dois anos após a introdução do molusco. Recentemente, de uma amostra de 483 espécimes coletados em junho de 2013, 101 (21%) apresentavam-se infectados pelo parasito. Os potenciais impactos ambientais ocasionados pela ocorrência deste parasito podem estar sendo subestimados no país, sendo necessárias ações visando o monitoramento e controle tanto do parasito quanto do molusco.


Subject(s)
Animals , Snails/parasitology , Heterophyidae/physiology , Host-Parasite Interactions , Brazil , Introduced Species , Metacercariae/growth & development , Metacercariae/physiology , Heterophyidae/growth & development
10.
Arq. bras. med. vet. zootec ; 69(1): 22-28, jan.-fev. 2017. ilus
Article in Portuguese | LILACS, VETINDEX | ID: biblio-834073

ABSTRACT

O presente trabalho utilizou oito pulmões de tamanduá-bandeira (Myrmecophaga tridactyla), jovens e adultos, de ambos os sexos, provenientes do Setor de Atendimento Clínico Cirúrgico de Animais Selvagens (SACCAS), do Hospital Veterinário "Dr. Halim Atique"( Unirp, localizado na cidade de São José do Rio Preto, estado de São Paulo. Os animais foram encaminhados ao Laboratório de Anatomia Veterinária, onde se procedeu à separação dos órgãos, à lavagem em água corrente e à retirada do excesso de sangue, para serem fixados em formol a 10%. Após a fixação dos pulmões, estes foram dissecados para identificar os segmentos. Os animais apresentaram lobação pulmonar à direita em cranial, médio, caudal e acessório, sendo o lobo cranial dividido em partes cranial e caudal. A lobação pulmonar à esquerda apresentou cranial e caudal em 100% dos animais. Por sua vez, o lobo cranial, em 87,5%, dividiu-se em partes cranial e caudal, e em 12,5% não houve divisão. O pulmão direito apresentou maior tamanho do que o pulmão esquerdo. A árvore brônquica apresentou os brônquios principais direito e esquerdo, os quais se ramificam em brônquios lobares, que dão origem aos segmentos bronquiopulmonares. Em relação à segmentação bronquiopulmonar, foram observadas, à direita, de 14 (12,5%) a 18 segmentos (25%) e, à esquerda, de 10 (25%) a 30 segmentos (12,5%).(AU)


The study used eight lungs of giant anteaters (Myrmecophaga tridactyla), youth and adults of both sexes, from the Clinical Surgical Care Industry Wildlife (SACCAS), Veterinary Hospital "Dr. Halim Atique "- Unirp, located in the city of São José do Rio Preto, São Paulo. The animals were sent to the Veterinary Anatomy Laboratory, where organs were separated and washed under running waterto remove excess blood, then fixed in 10% formalin. After fixation, lungs were dissected to identify the segments. The animals have pulmonary lobations right cranial, middle, caudal and accessory; and the cranial lobe divided into cranial and caudal parts. Pulmonary lobation in the left cranial and caudal form were present in 100% of animals. The cranial lobe was evident in 87.5%, divided into cranial and caudal part, and 12.5% with no division. The right lung was shown to be larger than the left lung. Bronchial tree presented the main right and left bronchial tubes that branch into lobar bronchi that give rise to bronquiopulmonares segments. In relation to bronquiopulmonar targeting, 14 (12.5%) to 18 right segments (25%) and 10 (25%) to 30 (12.5%) left segments.(AU)


Subject(s)
Animals , Bronchi/anatomy & histology , Lung/anatomy & histology , Cingulata/anatomy & histology , Animals, Wild/anatomy & histology , Body Weights and Measures/veterinary
11.
Rev. bras. plantas med ; 17(4): 592-598, out.-dez. 2015. graf
Article in English | LILACS | ID: lil-763228

ABSTRACT

ABSTRACTIn this study, ethanol-water extracts of pequi fruit peel were fractionated in order to identify and quantify the major antioxidant present in it. The fractions were subjected to liquid-liquid phase extraction and silica-gel column chromatography, and antioxidant activity was monitored using the 2,2-diphenyl-1-picrylhydrazyl radical-scavenging assay. The purity of the fractions was evaluated using thin-layer chromatography and high-performance liquid chromatography (HPLC). The substance with antioxidant property was identified through the analysis in a liquid chromatography-mass spectroscopy fragmentation and was quantified using HPLC. After the Silica-gel fractionation, it was identified a fraction with high antioxidant activity and purity, which contained gallic acid as the main compound. The gallic acid was found at the amount of 26.54 ± 1.13 mg/g of the dry mass of the pequi fruit peel. Because the quantifications were performed using crude ethanol-water extract, it was suspected that gallic acid was present in a free form. Thus, pequi fruit peel may serve as an attractive alternative of feedstock for natural antioxidant production. Moreover, the results obtained in this study emphasize the value of the pequi plant, and suggests improved opportunities for families that use this fruit`s products.


RESUMOExtratos hidroetanólicos da casca do fruto do pequi foram fracionados a fim de identificar e quantificar o principal antioxidante presente. Frações do extrato foram submetidas ao particionamento líquido-líquido e fracionamento em coluna de sílica gel. As atividades antioxidantes das frações foram monitoradas usando ensaio de redução do radical 2,2-difenil-1-picrilhidrazila e a pureza das frações foi avaliada em cromatografia de camada delgada e cromatografia líquida de alta eficiência (CLAE). A substância com propriedades antioxidantes foi identificada através da análise em sistema de cromatografia líquida associada à espectrometria de massas e foi quantificada em HPLC. Após o fracionamento identificou-se uma fração com alta atividade antioxidante e pureza, contendo ácido gálico como o composto principal. Ácido gálico foi encontrado em concentrações de 26,54 ± 1,13 mg/g de massa seca. Devido às quantificações terem sido realizadas no extrato hidroetanólico bruto, acredita-se que o ácido gálico esteja presente na forma livre. Assim, a casca do fruto pequi pode servir como interessante alternativa de matéria prima para a produção desse antioxidante natural. Além disso, esse resultado enfatiza o valor da planta do pequi e sugere oportunidades para as famílias que utilizam produtos de pequi.


Subject(s)
Antioxidants/pharmacology , Ericales/metabolism , Biological Products/classification , Chromatography, High Pressure Liquid/instrumentation , Plant Extracts/analysis
12.
Indian J Cancer ; 2015 Oct-Dec; 52(4): 544-545
Article in English | IMSEAR | ID: sea-176270
13.
Odontol. pediatr. (Lima) ; 14(1): 67-75, ene.-jul.2015. ilus, tab
Article in Spanish | LIPECS, LILACS, LIPECS | ID: lil-790458

ABSTRACT

La maloclusión pseudoclase III es caracterizada par un desequilibrio funcional que, por lo general, resulta de contactos oclusales prematuros que causan un desplazamiento funcional anterior de la mandíbula. Estos casos, si no son tratados en una etapa inicial de desarrollo, pueden generar interferencias en el crecimiento normal de las bases óseas y resultar en una deformidad facial. Este papel conlleva a la selecci6n de un aparato apropiado, tomando cuenta opciones actuales, para una intervenci6n temprana en el desarrollo de maloclusiones de clase III. El uso del aparato progenico en este tipo de maloclusión, permite la correcci6n dental en pocos meses y una estabilidad terapéutica de la mandíbula mesio-posicionada fomentando un crecimiento esquelético favorable en una niña de 5,6 años de edad que acude a la clínica de postgrado del Instituto Latino Americano de Investigaci6n y Enseñanza Odontológica (ILAPEO) en Curitiba, Brasil...


Pseudo Class III malocclusion is a functional imbalance that generally results from premature occlusal contacts that causes a functional anterior displacement of the mandible. These cases, if not treated at an early stage of development, may interfere in normal growth of bone bases, resulting in facial deformity. This paper suggests the selection of an adequate appliance considering the available possibilities for early intervention of Class III malocclusion. The use of the progenic appliance in such dental malocclusion allows correction in a few months and therapeutic stability mesio-positioned mandible encouraging favorable skeletal growth in a child of 5.6 years of age who came to the Postgraduate Clinic of the Latin American Institute of Dental Research and Education (ILAPEO) in Curitiba, Brazil...


Subject(s)
Humans , Female , Child, Preschool , Malocclusion, Angle Class III , Orthodontics, Corrective , Brazil
14.
Arq. bras. med. vet. zootec ; 67(3): 716-722, May-Jun/2015. tab
Article in Portuguese | LILACS | ID: lil-753917

ABSTRACT

O presente estudo avaliou equídeos de 19 fazendas da região do Pantanal Mato-Grossense, sendo 121 equídeos testados pela reação em cadeia pela polimerase (PCR), para detectar fragmentos dos genes dos seguintes gêneros: Babesia, Theileria, Anaplasma, Ehrlichia, e Neorickettsia, e pela reação de imunofluorescência indireta (RIFI), para detectar anticorpos anti-Ehrlichia spp. Das amostras testadas na PCR, 17 (14,0%) animais de nove (47,3%) fazendas foram positivos. Das amostras positivas, 16 foram 100% idênticas a sequencias de Theileria equi e uma foi 99% similar à sequência de Babesia caballi, todas disponíveis no GenBank. Pela RIFI, 48 (39,6%) equídeos foram soropositivos para antígenos de E. canis, sendo 40 (83,3%) amostras com títulos de 40 e oito (16,6%) com títulos de 320. Todas as fazendas avaliadas (100%) apresentaram equídeos soropositivos. Os resultados do presente estudo demonstram que T. equi e B. caballi infectam equinos na região, e a presença de anticorpos anti-Ehrlichia spp. indica a circulação de espécies antigenicamente relacionadas aos gêneros Ehrlichia e Anaplasma, apesar de a negatividade nos exames de PCR indicar provável processo crônico desses agentes.


The present study evaluated 121 equids from 19 ranches in the Pantanal region of Mato Grosso State through Polimerase Chain Raction (PCR) to detect Babesia, Theileria, Anaplasma, Ehrlichia and Neorickettsia partial genes and the Imunofluorescent Antibody Test (IFAT) to evaluate anti-Ehrlichia spp. antibodies. From the total tested in PCR, 17 (14.0%) equids from 9 (47.3%) farms were positive, and 16 yielded amplicons 100% identical to Theileria equi and one presented 99% similarity to Babesia caballi available on GenBank. Forty eight (39.6%) equids were positive by IFAT and 40 showed titers of 40 (83.3%) and 8 showed titers of 320 (16.6%). All ranches (100%) presented seropositive equids. Our results showed that T. equi and B. caballi are infecting equids in the region and the presence of anti-Ehrlichia antibodies suggests that species closely related to the genera Ehrlichia and Anaplasma are circulating among the equid local population. Moreover, the negative results in PCR is possibly related to the chronic infection phase.


Subject(s)
Animals , Antibodies, Bacterial , Babesia/isolation & purification , Ehrlichia/immunology , Equidae/microbiology , Theileria/isolation & purification , Tick Infestations/veterinary , Polymerase Chain Reaction/veterinary , Fluorescent Antibody Technique, Indirect/veterinary
15.
Int. j. morphol ; 32(4): 1243-1247, Dec. 2014. ilus
Article in Spanish | LILACS | ID: lil-734665

ABSTRACT

La leucemia linfoblástica aguda (LLA) es la neoplasia maligna hematooncólogica más frecuente en pacientes pediátricos contando hasta 75% de las leucemias y 32-35% del total de cánceres infantiles. Aunque la LLA es considerada una enfermedad con base genética, es cada vez más evidente que alteraciones epigenéticas desempeñan un rol central en su patogénia y progresión. La hipermetilación de regiones promotoras de genes es asociada con la pérdida de función génica. El gen supresor de tumores p53 (GST), es uno de los principales genes en el ciclo celular y apoptosis. El objetivo de este trabajo fue determinar el estado de metilación en la región del promotor-exón 1 del GST p53 y la asociación con la supervivencia en menores de 15 años con LLA. Se analizaron 40 pacientes provenientes de la Región de la Araucanía-Chile. La hipermetilación del p53 se determinó combinando enzimas de restricción sensibles a metilación (HpaII y EcoR II) y reacción en cadena de la polimerasa. Los resultados indicaron que 15/40 casos (37,5%) presentaron hipermetilación. Se encontró una diferencia estadística en la supervivencia según estado de metilación de p53 en el grupo de niñas (p=0,02). Considerando el total de pacientes, una tendencia a mejor supervivencia cuando los recuentos de leucocitos fueron <30.000/mm3 (p=0,08). Se encontró frecuentemente hipermetilado el gen p53 en la región del promotor-exon1. Esto indicaría que la hipermetilación del GST p53 puede ser un evento importante en la patogénesis de la LLA.


Acute lymphoblastic leukemia (ALL) is the most common hematology oncology malignancy in pediatric patients counting up to 75% of leukemias and 32­35% of all childhood cancers. Although ALL is considered a disease with a genetic basis, it is increasingly clear that epigenetic alterations play a central role in the pathogenesis and work was to determine the methylation status in promoter-exon1 of the TSG-p53 and association with survival in children under 15 years with ALL. In our study 40 patients from the Araucanía Region, Chile were analyzed. Hypermethylation of p53 was determined by combining restriction enzymes sensitive to methylation (HpaII and EcoR II) and polymerase chain reaction. Results indicated that 15/40 cases (37.5%) showed hypermethylation. Statistical difference was found in survival according to p53 methylation status in the girls group (p=0.02). Considering all patients, there was a trend to improved survival when leukocyte counts were <30.000/ul (p=0.08). We found the p53 gene frequently hypermethylated in the promoter-exon1 region. This would indicate that TSG p53 hypermethylation may be an important event in the pathogenesis of ALL.


Subject(s)
Humans , Male , Female , Infant , Child, Preschool , Child , Adolescent , Genes, p53 , DNA Methylation , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics , Bone Marrow , DNA Restriction Enzymes , Survival Analysis , Promoter Regions, Genetic , Epigenesis, Genetic , Age and Sex Distribution , Multiplex Polymerase Chain Reaction , Leukocyte Count
16.
Int. j. morphol ; 31(3): 973-979, set. 2013. ilus
Article in Spanish | LILACS | ID: lil-694988

ABSTRACT

La leucemia linfoblástica aguda (LLA), es la neoplasia mas frecuente en la población infantil. Se manifiesta por una perdida de diferenciación de progenitores linfoides produciendo un aumento de células inmaduras. La hipermetilación en la región promotora de genes supresores de tumores (GST) puede producir un silenciamiento génico que le proporciona a la célula leucémica una ventaja proliferativa o la previene de la apoptosis. Se estudia el estado de hipermetilación de 4 GST involucrados en la apoptosis: APAF1, ASPP1, p73 y FHIT y su asociación con la sobrevida de pacientes menores de 15 años con diagnóstico de LLA. Se analizaron 38 muestras de médula ósea mediante modificación con bisulfito del ADN y reacción en cadena de la polimerasa especifica de metilación (MSP). El rango de edad al diagnóstico fue de 10 meses a 13,8 años. La sobrevida global fue de 69 por ciento a los 5 años. El 81,5 por ciento de los pacientes tuvo al menos un gen hipermetilado. La frecuencia de metilación observada fue: APAF1 68,4 por ciento, FHIT 56,4 por ciento, p73 42 por ciento y ASPP1 18,4 por ciento. La asociación entre hipermetilación y grupo <5 años y 5 años fue: Global p=0,20, APAF1 p=0,03, FHIT p=0,51, p73 p=0,51 y ASPP1 p=0.67. Las curvas de sobrevida se calcularon según frecuencia de hipermetilación de cada gen: APAF1 p=0,05, FHIT p=0,31, p73 p=0,98 y ASPP1 p=0,82. La alta frecuencia de hipermetilación obtenida reafirma la participación de la metilación en la región promotora de GST en la patogénesis de la LLA. La hipermetilación del gen APAF1 fue muy frecuente y se asoció significativamente a la sobrevida del grupo de estudio, mostrando a este gen como un factor predictivo de mal pronostico en pacientes con LLA.


Acute lymphoblastic leukemia (ALL) is the most common malignancy in children. It is manifested by a loss of differentiation of lymphoid progenitors, producing an increase of immature cells. Hypermethylation in promoter region of tumor suppressor genes (GST) may produce a gene silencing that provides a leukemic cell a proliferative advantage or prevent apoptosis. We studied the hypermethylation status of 4 GST involved in apoptosis: APAF1, ASPP1, p73 and FHIT and its association with survival of patients <15 years diagnosed with ALL. We analyzed 38 samples of bone marrow by DNA bisulfite modification and chain reaction methylation-specific polymerase (MSP). The mean age at diagnosis was 10 months to 13.8 years. Overall survival was 69 percent at 5 years. 81.5 percent of patients had at least one hypermethylated gene. The frequency observed was: APAF1 68.4 percent, 56.4 percent FHIT, p73 ASPP1 42 percent and 18.4 percent. The association between hypermethylation and group <5 years and 5 years was: Global p = 0.20, APAF1 p = 0.03, FHIT p = 0.51, p73 p = 0.51, ASPP1 p = 0.67. Survival curves were calculated by frequency of hypermethylation of each gene: APAF1 p = 0.05, p = 0.31 FHIT, p73 p = 0.98 and ASPP1 p = 0.82. The high frequency of hypermethylation obtained confirms enrollment of methylation in the promoter region of GST in the pathogenesis of ALL. APAF1 gene hypermethylation was very frequent and was significantly associated with survival in the study group, showing this gene as a predictor of poor prognosis in patients with ALL.


Subject(s)
Humans , Male , Adolescent , Female , Infant, Newborn , Infant , Child, Preschool , Child , DNA Methylation , Genes, Tumor Suppressor , Precursor Cell Lymphoblastic Leukemia-Lymphoma/genetics , Apoptosis , Polymerase Chain Reaction , Survival Analysis
17.
Braz. j. med. biol. res ; 46(9): 752-757, 19/set. 2013. graf
Article in English | LILACS | ID: lil-686571

ABSTRACT

One of the challenges of the postgenomic era is characterizing the function and regulation of specific genes. For various reasons, the early chick embryo can easily be adopted as an in vivo assay of gene function and regulation. The embryos are robust, accessible, easily manipulated, and maintained in the laboratory. Genomic resources centered on vertebrate organisms increase daily. As a consequence of optimization of gene transfer protocols by electroporation, the chick embryo will probably become increasingly popular for reverse genetic analysis. The challenge of establishing chick embryonic electroporation might seem insurmountable to those who are unfamiliar with experimental embryological methods. To minimize the cost, time, and effort required to establish a chick electroporation assay method, we describe and illustrate in great detail the procedures involved in building a low-cost electroporation setup and the basic steps of electroporation.


Subject(s)
Animals , Chick Embryo , Electroporation/economics , Electroporation/instrumentation , Electroporation/methods , Gene Expression Regulation/genetics , Gene Transfer Techniques/instrumentation , Electrodes , Equipment Design , Green Fluorescent Proteins
18.
Rev. paul. pediatr ; 31(3): 411-415, set. 2013. tab, ilus
Article in English | LILACS | ID: lil-687976

ABSTRACT

OBJECTIVE To present a case of a patient with clinical and radiological features of reexpansion pulmonary edema, a rare and potentially fatal disease. CASE DESCRIPTION An 11-year-old boy presenting fever, clinical signs and radiological features of large pleural effusion initially treated as a parapneumonic process. Due to clinical deterioration he underwent tube thoracostomy, with evacuation of 3,000 mL of fluid; he shortly presented acute respiratory insufficiency and needed mechanical ventilation. He had an atypical evolution (extubated twice with no satisfactory response). Computerized tomography findings matched those of reexpansion edema. He recovered satisfactorily after intensive care, and pleural tuberculosis was diagnosed afterwards. COMMENTS Despite its rareness in the pediatric population (only five case reports gathered), the knowledge of this pathology and its prevention is very important, due to high mortality rates. It is recommended, among other measures, slow evacuation of the pleural effusion, not removing more than 1,500 mL of fluid at once. .


OBJETIVO Relatar caso de paciente con cuadro clínico y radiológico de edema pulmonar de reexpansión, patología rara y potencialmente fatal. DESCRIPCIÓN DEL CASO Niño de 11 años con fiebre y cuadro clínico-radiológico de derrame pleural voluminoso, inicialmente tratado como parapneumónico. Después de la compensación clínica, se realizó drenaje torácico, con salida de 3000mL de líquido. Evolucionó rápidamente con insuficiencia respiratoria aguda, necesitando ventilación mecánica. Tuvo dos extubaciones mal sucedidas y, debido a la evolución atípica, se realizó tomografía computadorizada, cuyos hallazgos fueron compatibles con edema de reexpansión. Después de soporte intensivo, evolucionó satisfactoriamente y, posteriormente, se diagnosticó tuberculosis pleural. COMENTARIOS Es importante el conocimiento de la patología, aunque sea rara en la población pediátrica (encontrados solamente cinco casos descriptos), para prevención, una vez que la tasa de mortalidad es muy alta. Se recomienda, entre otras medidas, que el vaciamiento de un derrame pleural sea lento y que el volumen total retirado no sobrepase 1500mL. .


OBJETIVO Relatar caso de paciente com quadro clínico e radiológico de edema pulmonar de reexpansão, patologia rara e potencialmente fatal. DESCRIÇÃO DO CASO Menino de 11 anos com febre e quadro clínico-radiológico de derrame pleural volumoso, inicialmente tratado como parapneumônico. Após descompensação clínica, realizou-se drenagem torácica, com saída de 3.000mL de líquido. Evoluiu rapidamente com insuficiência respiratória aguda, necessitando de ventilação mecânica. Teve duas extubações malsucedidas e, devido à evolução atípica, realizou-se tomografia computadorizada, cujos achados foram compatíveis com edema de reexpansão. Após suporte intensivo, evoluiu satisfatoriamente e, posteriormente, foi diagnosticado com tuberculose pleural. COMENTÁRIOS É importante o conhecimento da patologia, ainda que seja rara na população pediátrica (encontrados apenas cinco casos descritos), para prevenção, visto que a taxa de mortalidade é muito alta. Recomenda-se, entre outras medidas, que o esvaziamento de um derrame pleural seja lento e que o volume total retirado não ultrapasse 1.500mL. .


Subject(s)
Humans , Male , Child , Pleural Effusion , Pulmonary Edema , Tuberculosis, Pleural/diagnosis
19.
Rev. paul. pediatr ; 31(1): 121-123, mar. 2013.
Article in Portuguese | LILACS | ID: lil-671668

ABSTRACT

OBJETIVO: Alertar os pediatras sobre a necessidade de investigar criteriosamente a etiologia de eventos com aparente risco de morte recorrente. Não foram encontrados relatos associando tais eventos à miastenia congênita. DESCRIÇÃO DO CASO: Lactente de sete meses apresentando história de eventos com aparente risco de morte recorrente foi internado para investigação. Durante a internação, apresentou cianose e dispneia progressiva, com necessidade de ventilação mecânica por três dias. Após a melhora clínica, e tendo sido descartadas as hipóteses de doença do refluxo gastroesofágico e aspiração pulmonar como desencadeantes, notou-se ptose palpebral bilateral, hipotonia apendicular e choro fraco, que conduziram à suspeita clínica de miastenia congênita. Após confirmação do diagnóstico, foi mantido tratamento ambulatorial com piridostigmina, com recuperação nutricional e neurológica, sem novos eventos com aparente risco de morte nos três anos seguintes. COMENTÁRIOS: A investigação minuciosa das causas de eventos com aparente risco de morte pode levar a diagnósticos menos frequentes que exigem tratamento específico, como a miastenia congênita.


OBJECTIVE: To alert pediatricians about the importance of a careful investigation on recurrent apparent life-threatening events. Reports of the association of these events with congenital myasthenic syndromes were not found. CASE DESCRIPTION: A seven-month-old infant with recurrent apparent life-threatening events was admitted for investigation. During hospital stay, she presented cyanosis and respiratory failure, requiring mechanical ventilation for three days. After clinical improvement, hypotheses of gastroesophageal reflux and pulmonary aspiration were ruled out. The presence of eyelid ptosis, general hypotonia and weak crying led to the suspicion of congenital myasthenia, which was confirmed. Treatment with oral piridostigmine led to neurological and nutritional normalization, without any other apparent life-threatening event during the next three years. COMMENTS: The careful etiological investigation of apparent life-threatening events may lead to rare diagnosis that requires specific treatments, such as congenital myasthenia.


OBJETIVO: Alertar a los pediatras sobre la necesidad de investigar criteriosamente la etiología de eventos con aparente riesgo de muerte recurrente. No se encontraron relatos asociando tales eventos a la miastenia congénita. DESCRIPCIÓN DEL CASO: Lactante de siete meses presentando historia de eventos con aparente riesgo de muerte recurrente fue internado para investigación. Durante la internación, presentó cianosis y disnea progresiva, con necesidad de ventilación mecánica por tres días. Después de la mejora clínica, y habiendo sido rechazadas las hipótesis de enfermedad del reflujo gastroesofágico y aspiración pulmonar como desencadenantes, se notó ptosis palpebral bilateral, hipotonía apendicular y lloro débil, que condujeron a la sospecha clínica de miastenia congénita. Después de la confirmación del diagnóstico, se mantuvo el tratamiento ambulatorial con piridostigmina, con recuperación nutricional y neurológica, sin nuevos eventos con aparente riesgo de muerte en los tres años siguientes. COMENTARIOS: La investigación minuciosa de las causas de eventos con aparente riesgo de muerte puede llevar a diagnósticos menos frecuentes que exigen tratamiento específico, como la miastenia congénita.


Subject(s)
Female , Humans , Infant , Brief, Resolved, Unexplained Event/etiology , Myasthenic Syndromes, Congenital/complications , Myasthenic Syndromes, Congenital/diagnosis , Recurrence
20.
Rev. chil. pediatr ; 84(1): 42-50, feb. 2013. tab
Article in Spanish | LILACS | ID: lil-677318

ABSTRACT

Introduction: there are several epidemiological studies regarding the incidence of post-traumatic stress disorder (PTSD) in children exposed to natural disasters. Objective: to describe the prevalence of PTSD in a school-age population in a coastal town from the Maule Region, 8 months after the earthquake/tsunami in february 2010, and to compare differences among PTSD groups of symptom (re-experiencing, avoidance and activation) according to demographic variables such as age, grade, gender and family type. Methodology: the Child PTSD Symptom Scale (CPSS), validated in Chile in 2009, was used in 89 children between 3rd and 6th grade, corresponding to 94.7 percent of the children enrolled in the local school in such classes. Data are analyzed by gender, age, grade and type of family. 89 surveys were applied, 59.6 percent were male and 40.4 percent female aged 8-13. Results: 40.4 percent of children had symptoms consistent with PTSD, with higher incidence in women and younger children; the most significant association among women was age. Both groups (women and young children) presented the highest scores on all group of symptoms. There were no differences by type of family. Conclusions: the incidence of PTSD measured by CPSS scale in the study population was 40.4 percent, considered to be among the highest percentages reported in the international literature.


Introducción: existen diversos datos epidemiológicos respecto a la incidencia de Trastorno por Estrés Pos-traumático (TEPT) en niños expuestos a desastres naturales. Objetivo: describir la prevalencia de TEPT en una población infantil escolarizada de una localidad costera de la Región del Maule después de 8 meses de ocurrido el terremoto/maremoto de febrero/2010, y comparar las diferencias entre grupos de síntomas del TEPT (reexperimentación, evitación y activación) según variables demográficas, como edad, curso, sexo y tipo de familia. Metodología: se aplicó la escala Child PTSD Symptom Scale (CPSS) validada en Chile el año 2009, a 89 niños de 3° a 6° básico lo que corresponde al 94,7 por ciento de los niños matriculados en la escuela de la localidad en dichos cursos. Se analizan los datos según sexo, edad, curso y tipo de familia. Se aplicaron 89 encuestas, 59,6 por ciento eran varones y 40,4 por ciento mujeres de 8 a 13 años de edad. Resultados: el 40,4 por ciento del total de niños tuvo una evaluación compatible con TEPT, con mayor incidencia en mujeres y niños de menor edad, siendo la edad un factor de asociación significativa en las mujeres. Ambos grupos (mujeres y niños más pequeños) presentaron mayores puntajes en todos los grupos sintomáticos. No se encontraron diferencias según el tipo de familia. Conclusiones: la incidencia de TEPT medida a través de la escala CPSS en la población estudiada fue de 40.4 por ciento, encontrándose entre las más altas reportadas en la bibliografía internacional.


Subject(s)
Humans , Male , Adolescent , Female , Child , Earthquakes , Stress Disorders, Post-Traumatic/epidemiology , Age and Sex Distribution , Chile , Prevalence , Psychiatric Status Rating Scales , Stress Disorders, Post-Traumatic/diagnosis
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