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1.
Acta Pharmaceutica Sinica ; (12): 2295-2301, 2021.
Article in Chinese | WPRIM | ID: wpr-887036

ABSTRACT

Medicinal and edible Armeniacae Semen Amarum (ASA) is susceptible to fungal contamination because it is rich in oil and other nutrients. In this study, the fungal community diversity in ASA samples was analyzed based on a DNA metabarcoding technique to provide evidence for its safe use. Twelve batches of ASA samples samples from four medicinal material markets and three processing approaches were collected. Total DNA was extracted, the ITS2 sequences were amplified, and high-throughput sequencing was performed using the Illumina MiSeq PE300 platform. The results show that Ascomycota was the most dominant fungus in ASA samples. The predominant genus in sample SW1_P was Diutina, whereas the most predominant genus in the other samples was Aspergillus. Three harmful fungi were identified, namely, Aspergillus flavus, Wallemia sebi, and Rhizopus arrhizus. In addition, significant differences were observed in the relative abundance of Botryosphaeriales and Alternaria in ASA samples from different collection sites. Meanwhile, there were significant differences in the relative abundance of Hypocreales and Cladosporium in ASA samples from different processing approaches. In summary, the DNA metabarcoding technique can effectively clarify the fungal community diversity and quickly detect potential toxigenic fungi in ASA samples, thus providing a warning for mycotoxin contamination.

2.
Article in Chinese | WPRIM | ID: wpr-879003

ABSTRACT

Arecae Semen, as the first place among "Four South Medicines" in China, has great dual-use value of medicine and food. The research of Arecae Semen was mainly focused on the active ingredients and efficacy value, and its potential safety hazards were also concerned. Until now, there is still a lack of clear boundaries between medicine and food, resulting in its safety cannot be guaranteed. Therefore, it is of great significance to establish clear boundaries of medicine and food use and health risk assessment. In this paper, the differences of pretreatment and application methods of Arecae Semen were analyzed, and the research progress of Arecae Semen in chemical composition identification and toxicology research and safety evaluation were reviewed emphatically. Finally, the differences of quality control and safety evaluation of Arecae Semen in pharmacopoeias or standards were analyzed at home and abroad. It was expected to provide reference value for quality control, safety evaluation and international standardization research of Arecae Semen.


Subject(s)
Areca , China , Drugs, Chinese Herbal/adverse effects , Seeds , Semen
3.
Article in Chinese | WPRIM | ID: wpr-851778

ABSTRACT

The issues of mycotoxin contamination in herbal materials have been frequently reported in recent years, which has attracted broad attention at home and abroad. Mycotoxins can trigger hepatotoxicity, nephrotoxicity, and other significant toxic effects, threatening the consumer health seriously. Aspergillus species are one of the main sources of fungal contamination in herbal materials. The rapid and accurate identification of Aspergillus species in herbal materials can provide early warning of mycotoxin contamination, which is of great significance for ensuring the efficacy and safety of herbal materials. In this study, the research progress on the classification and discrimination of Aspergillus species were systematically summarized. Moreover, the identification of Aspergillus species in herbal materials was also reviewed. Meanwhile, we explored the prospect of identifying Aspergillus species in Chinese herbal medicines using high-throughput sequencing.

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