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1.
Journal of Leukemia & Lymphoma ; (12): 340-345, 2020.
Article in Chinese | WPRIM | ID: wpr-862842

ABSTRACT

Objective:To explore the expression of Fbxw7 protein and its clinical significance in diffuse large B-cell lymphoma (DLBCL), and to provide a basis for prognostic judgement and searching the new therapeutic targets of DLBCL.Methods:A total of 72 patients with newly diagnosed DLBCL who received immunohistochemical detection of c-myc protein from January 2011 to September 2017 in Cancer Hospital Affilicoted to Zhengzhou University were enrolled. The paraffin-embedded specimens after lymph node biopsy and the clinical data of patients were also collected. At the same time, 22 samples of lymph node reactive hyperplasia were selected as the control group. Immunohistochemical method was used to detect the expression of Fbxw7 protein in DLBCL tissues and control tissues. The relationship between the expression of Fbxw7 protein and c-myc protein, the association of Fbxw7 protein expression with DLBCL patients' clinicopathological characteristics, efficacy and prognosis were analyzed.Results:The positive rate of Fbxw7 protein in DLBCL tissues was lower than that in control tissues, and the difference was statistically significant [63.89% (46/72) vs. 86.36% (19/22), χ 2 = 3.990, P = 0.046]. Among DLBCL patients, the positive rate of Fbxw7 protein in non-germinal center B cell (non-GCB) group was lower than that in germinal center B cell (GCB) group, and the difference was statistically significant [48.15% (13/27) vs. 73.33% (33/45), χ 2 = 4.639, P = 0.031]. There were no statistically significant differences in the positive rate of Fbxw7 protein among patients with different age, gender, neoplasm staging, international prognostic index (IPI) scores, B symptom, Eastern Cooperative Oncology Group (ECOG) score, lactate dehydrogenase (LDH) level, β 2 microglobulin level, and therapeutic efficacy after initial treatment (all P > 0.05). In DLBCL tissues, the expression of Fbxw7 and c-myc protein was negatively correlated ( r = -0.255, P = 0.031). The 3-year overall survival (OS) rate and 3-year progression-free survival (PFS) rate (88.3% and 82.0%) of the Fbxw7 positive group were higher than those of the Fbxw7 negative group (70.2% and 60.1%). Cox multivariate analysis showed that the down-regulation of Fbxw7 protein expression was an independent risk factor affecting OS and PFS in DLBCL patients ( HR = 3.656, 95% CI 1.055-12.674, P = 0.041; HR = 2.897, 95% CI 1.092-7.688, P = 0.033). Conclusions:The expression of Fbxw7 protein and c-myc protein in DLBCL patients is negatively correlated. Fbxw7 protein is down-regulated in DLBCL, and it is more obvious in non-GCB subtype. The down-regulated expression of Fbxw7 protein is related to the poor prognosis of DLBCL, and Fbxw7 may become a new therapeutic target of DLBCL.

2.
Article in Chinese | WPRIM | ID: wpr-641053

ABSTRACT

Background Idiopathic orbital inflammatory pseudotumor (IOIP) is a commom orbital disease,with serious eye symptoms and replase tendency,and its pathogenesis is still unclear.Nuclear factor-κB (NF-κB)-related proteins participate in many important pathophysiological process,however,whether NF-κB plays a role in the IOIP process is worthy of attention.Objective This study was to explore the roles of NF-κB pathway in IOIP pathogenesis.Methods Twenty-four IOIP specimens were collected during surgery in Beijing Tongren Hospital from September 2010 to May 2016.The histopathological characteristics of IOIP were examined by hematoxylin and eosin staining.The expression and location of NF-κB/p65,p-p65,p50 and inhibitor of κB (IκB-ot) were detected by immunohistochemistry and verified by immunocytochemistry and Western blot assay.Results The histopathological features of IOIP were numerous small lymphocyte infiltraion and fibrous tissue proliferation,and a lot of epithelioid cells were seen in lacrimal gland-involved specimens.NF-κB/p65 was positively expressed in the cytoplasm of all 24 specimens and the nucleus in 15 specimens with the expressing rate of 62.5%.p50 was expressed in the cytoplasm in 22 specimens with the expressing rate of 91.7% and in the nucleus in 17 specimens with the expressing rate of 70.8%.The positive expression of p-p65 was found in 22 specimens with the expressing rate of 91.7%,and IκB-α was expressed in the cytoplasm of 11 specimens with the expressing rate of 45.8%.These results were confirmed by immunocytochemistry and Western blot assay.Conclusions NF-κB pathway is activiated during IOIP process,and NF-κB pathway may be involved in the pathogenesis of IOIP.

3.
Article in English | WPRIM | ID: wpr-281415

ABSTRACT

Objective The aim of this study is to investigate the cerebral cortical thickness changes in type 2 diabetes mellitus (T2DM) using a whole brain cortical thickness mapping system based on brain magnetic resonance imaging (MRI).Methods High resolution three-dimensional T1-weighted fast spoiled gradient recalled echo MR images were obtained from 16 patients with T2DM, as well as from 16 normal controls. The whole brain cortical thickness maps were generated, and the cortical thickness of each brain region was calculated according to gyral based regions of interest (ROI) using an automated labeling system by the Freesurfer software. We compared mean cortical thickness at each brain region by the analysis of covariance with age and sex as covariates. The regional difference of the cortical thickness over the whole brain was compared by the analysis of surface-based cortical thickness.Results Mean cortical thicknesses analysis showed bilateral cerebrum in the patients with T2DM (left: 2.52±0.07 mm; right: 2.51±0.08 mm) were significant thinner than those in the normal controls (left: 2.56±0.09 mm; right: 2.56±0.09 mm) (both P<0.05). Regional cortical thinning in T2DM was demonstrated in the paracentral lobule, postcentral gyrus, lateral occipital gyrus, lingual gyrus, precuneus, superior temporal gyrus, middle temporal gyrus, inferior temporal gyrus and posterior cingulate gyrus, compared to the normal controls. The cortical thickness of left middle cingulate and right inferior temporal gyrus were negatively correlated with the disease course.Conclusion A widespread cortical thinning was revealed in patients with T2DM by the analysis of brain cortical thickness on MR. Our finding supports the idea that T2DM could lead to subtle diabetic brain structural changes.


Subject(s)
Aged , Cerebral Cortex , Pathology , Diabetes Mellitus, Type 2 , Diagnostic Imaging , Pathology , Female , Humans , Magnetic Resonance Imaging , Methods , Male , Middle Aged , Pilot Projects
4.
Article in Chinese | WPRIM | ID: wpr-461412

ABSTRACT

This study was aimed to explore clinical functions of consensus-based clinical practice guidelines (CB CPGs) in traditional Chinese medicine (TCM). Under the guidance of the State Administration of TCM, 42 TCM hospitals in China had conducted a questionnaire survey for 73 CB CPGs and 2993 questionnaires had been included and analyzed. According to the category of good, general and bad, the proportion of the good in improving safety performance was 69.43%, controlling cost was 58.57%, regulating medical behavior was 55.50%, improving clinical effectiveness was 46.74%. It was concluded that the CB CPGs had played a good role in improving safety performance and controlling cost in TCM clinical practice.

5.
Article in Chinese | WPRIM | ID: wpr-312631

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate early occult brain functional damage in patients with type 2 diabetes mellitus using resting-state functional magnetic resonance imaging (MRI).</p><p><b>METHODS</b>High-resolution three-dimensional T1-weighted fast spoiled gradient recalled echo MRI and resting-state functional MRI images were obtained from 18 patients with type 2 diabetes mellitus and 18 normal control subjects. Regional homogeneity (ReHo) map, amplitude of low-frequency fluctuation (ALFF) map, and functional connectivity map of the bilateral hippocampus and posterior cingulate gyrus were calculated and voxel-based analysis was performed using two-sample t-test.</p><p><b>RESULTS</b>In type 2 diabetic patients, decreased ReHo was deteted in the right thalamus, hippocampus, olfactory cortex and left putamen as compared with the normal controls. The decreased ALFF was found mainly in the left middle frontal gyrus, right supramarginal gyrus and middle occipital gyrus in the diabetic patients. The patients showed reduced functional connectivity between the bilateral hippocampus but not between the bilateral posterior gyrus and the other brain regions.</p><p><b>CONCLUSION</b>The occult brain damage is featured by decreased ReHo and ALFF in multiple brain regions and reduced functional connectivity between the bilateral hippocampus in type 2 diabetic patients.</p>


Subject(s)
Brain , Pathology , Brain Injuries , Brain Mapping , Case-Control Studies , Diabetes Mellitus, Type 2 , Pathology , Frontal Lobe , Gyrus Cinguli , Hippocampus , Humans , Imaging, Three-Dimensional , Magnetic Resonance Imaging
6.
Chinese Journal of Radiology ; (12): 811-815, 2013.
Article in Chinese | WPRIM | ID: wpr-442668

ABSTRACT

Objective To evaluate early occult brain damage by diffusion tensor imaging in patients with type Ⅱ diabetes mellitus.Methods High resolution three-dimensional T1-weighted fast spoiled gradient recalled echo MRI images and diffusion tensor imaging were obtained from 16 patients with type Ⅱ diabetes mellitus (T2DM) and 16 normal controls (NC).Average apparent diffusion coefficient (ADC) map and fractional anisotropy (FA) map were calculated and analyzed with voxel-based analysis.Analysis of covariance was performed to compare ADC and FA between T2DM and NC.Results Increased ADC values of gray matter were demonstrated in right superior temporal gyrus,middle frontal gyrus,anterior cingulate gyrus,middle cingulate gyrus,inferior occipital gyrus,and left lingual gyrus,operculum orbitale,fusiform gyrus and parahippocampal gyrus in T2DM (voxel numbers in clusters were 176-3125,P < 0.01).And increased ADC values of white matter were demonstrated in the regions of right middle temporal lobe,linual lobe,middle frontal lobe,calcarine gyrus,superior parietal lobe,and left operculum orbitale in T2DM patients compared with NC (voxel numbers in clusters were 200-781,P < 0.01).Decreased FA values of gray natter were mainly located in left thalamus,bilateral caudate nuclei and right cuneus in T2DM(voxel numbers in clusters were 210-1402,P <0.01).Decreased FA values of white matter were demonstrated in the regions of right cuneus and the body of corpus callosum in T2DM (voxel numbers in clusters were 206,253,P < 0.01).Conclusions The occult brain damage is demonstrated as increased ADC values and decreased FA values in regional brain parenchyma in type Ⅱ diabetes mellitus.Voxel-based analysis of diffusion tensor indices may be a simple and effective tool for the evaluation of occult brain damage in type]][diabetes mellitus.

7.
Article in Chinese | WPRIM | ID: wpr-322110

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the age-related changes in resting functional connectivity of the marginal division (MrD) of the neostriatum in healthy adults detected using resting-state functional magnetic resonance imaging (rs-fMRI).</p><p><b>METHODS</b>Forty-four healthy volunteers aged between 22 and 44 years (mean 30.36∓6.31 years) and 20 elderly volunteers aged between 48-83 years (mean 61.4∓10.25 years) underwent conventional MRI, 3D structural MRI and rs-fMRI. MrD was defined manually on the structural images for computation of the functional connectivity maps. The single group data were analyzed with independent sample t test, and the data of the two-group were examined by analysis of covariance with gender as the covariance.</p><p><b>RESULTS</b>In the young volunteers, the brain regions of functional connectivity related with the MrD were located in bilateral middle cingulate gyri, putamen, thalamus and amygdala, as compared with the brain regions of the bilateral putamen, thalamus, amygdala, insular cortex, rolandic operculum and right supramarginal gyrus in the elderly volunteers. Compared with the young volunteers, the elderly volunteers showed decreased functional connectivity related with the MrD in the bilateral middle cingulate gyri, parahippocampal gyri and left hippocampus.</p><p><b>CONCLUSION</b>An increased age is associated with alterations in the functional connectivity related with the MrD in healthy adults.</p>


Subject(s)
Adult , Age Factors , Aged , Aged, 80 and over , Female , Humans , Limbic System , Physiology , Magnetic Resonance Imaging , Methods , Male , Middle Aged , Neural Pathways , Rest , Young Adult
8.
Article in Chinese | WPRIM | ID: wpr-322050

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the structural connectivity between visual cortex and auditory cortex in healthy adults.</p><p><b>METHODS</b>Diffusion tensor imaging was performed to examine the brain of 21 healthy adult subjects. The structural connectivity was calculated based on fractional anisotropy (FA) value of the visual and auditory cortices, and fiber tracking was performed between the visual cortex and auditory cortex.</p><p><b>RESULTS</b>Positive structural connectivity was demonstrated between the bilateral visual cortices, and between the bilateral auditory cortices. Ipsilateral primary auditory cortex presented a negative structural connectivity with the ipsilateral visual cortex, and a positive structural connectivity with the contralateral visual cortex. A positive connectivity was demonstrated between the secondary auditory cortex and visual cortex. Tracking analysis showed fiber connectivity between the bilateral visual cortices, and between the ipsilateral auditory and visual cortices.</p><p><b>CONCLUSION</b>Intrinsic structural connectivity is present between the visual cortex and auditory cortex in the brain of healthy adults.</p>


Subject(s)
Adult , Auditory Cortex , Diffusion Tensor Imaging , Female , Humans , Male , Visual Cortex , Young Adult
9.
Article in Chinese | WPRIM | ID: wpr-319431

ABSTRACT

<p><b>OBJECTIVE</b>To elucidate the volume changes of cortical and subcortical reward circuitry in patients with type 2 diabetes mellitus.</p><p><b>METHODS</b>High-resolution three-dimensional T1-weighted fast spoiled gradient recalled echo MRI images were obtained from 16 patients with type 2 diabetes mellitus and 16 normal controls, and 11 type 2 diabetic patients also received the same MRI scans after insulin therapy for 1 year. Volumetric analysis was performed and analysis of covariance and paired t test were applied.</p><p><b>RESULTS</b>A decreased volume was found in the left insular lobe, left nucleus accumbens area, right hippocampus, putamen and amygdala in type 2 diabetic patients compared with normal controls (P<0.05). After insulin therapy for 1 year, an increased volume of bilateral cortical reward structures was observed (left, 33.65∓3.66 ml; right, 33.35∓4.25 ml) compared the baseline level (left, 31.45∓2.90 ml; right, 31.12∓2.97 ml) in diabetic patients (P<0.05). No significant volume change in the bilateral basal ganglia structures was found after insulin therapy for 1 year (P>0.05), and bilateral ventral diencephalon area showed an increased volume after the treatment (left, 3.26∓0.68 ml; right, 3.20∓0.78 ml) compared with the baseline (left, 2.96∓0.76 ml; right, 2.82∓0.90 ml)(P<0.05).</p><p><b>CONCLUSION</b>Type 2 diabetic patients have a decreased volume of the cortical and subcortical reward circuitry, and insulin therapy can reverse such changes and improve the damage of reward circuitry.</p>


Subject(s)
Aged , Cerebral Cortex , Pathology , Diabetes Mellitus, Type 2 , Drug Therapy , Pathology , Entorhinal Cortex , Pathology , Female , Humans , Insulin , Therapeutic Uses , Magnetic Resonance Imaging , Male , Middle Aged , Nucleus Accumbens , Pathology
10.
Article in Chinese | WPRIM | ID: wpr-592240

ABSTRACT

0.05). Conclusion Both acanthopanasia and hoelen have no mutagenic toxicity founded on the genetic damage of somatic cells,and have significant antagonistic effects on the genetic damage induced by cadmium sulfate,and they are good antimutagents.

11.
Article in Chinese | WPRIM | ID: wpr-546401

ABSTRACT

Objective To study the CT and MRI findings of adenoid cystic carcinoma(ACC) of the head and neck.Methods All 16 cases of ACC were verified by histopathology.Imaging data were analyzed retrospectively.Results The lesion mainly occurred in maxillary sinus in 5 cases,in middle ear and in the floor of mouth in 3 cases respectively,in nasopharynx and parotid in 2 cases respectively,in nasal cavity in 1 case.On CT,the lesion showed ill defined and irregular-shaped soft mass in 6 cases and associated with osteolytic bony destruction in 3 cases.On MR T1WI,the lesion showed isointensity compared to the muscle in 14 cases and slightly hypointensity in 2 cases.On T2WI,the lesion showed isointensity in 6 cases,hyperintensity in 10 cases,heterogeneous intensity in 9 cases.Postcontrast MR imagings demonstrated obvious enhancement in 15 cases,moderate enhancement in 1 case.Lesion with short T2 septum in 7 cases,all of them showed no enhancement.Conclusion CT is the optimal modality in showing osteolytic bony destruction.MRI can demonstrate the invaded extent of the lesions clearly.Combined imaging modalities can provide more comprehensive information in diagnosis and therapy of adenoid cystic carcinoma of the head and neck.

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