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1.
Article in English | WPRIM | ID: wpr-836715

ABSTRACT

Purpose@#This study aimed to determine the mediating effect of emotional intelligence on the relationship between the social support and job satisfaction of general hospital nurses. @*Methods@#The data of 197 nurses in general hospitals located in three different cities were collected for analysis. Collected data were analyzed using the t-test, ANOVA, Scheffé test, Pearson's correlation coefficients, and hierarchical multiple regression with SPSS/WIN 23.0. @*Results@#The social support of the subjects showed positive correlations with job satisfaction (r=.56, p<.001) and emotional intelligence (r=.42, p<.001). Emotional intelligence and job satisfaction were positively correlated (r=.54, p<.001). Emotional intelligence was found to perform the partial mediation in the relationship between social support and job satisfaction. @*Conclusion@#To improve job satisfaction, an organizational culture which enhances social support may be required as an intervention strategy, and programs should be developed to induce positive emotional intelligence.

2.
Article | WPRIM | ID: wpr-835792

ABSTRACT

Purpose@#This study considers the variables related to self-leadership of Korean nursing students and verifies the results of precedent studies through meta-analysis. @*Methods@#This study selected a total of 48 precedent studies regarding the investigation of the correlation between variables related to self-leadership of nursing students conducted between 2009 and 2019 and carried out a meta-analysis. @*Results@#For the effect size of variables related to self-leadership of nursing students, individual characteristics had a medium effect size (ESr=.49) and the effect size was larger for meta-cognition (ESr=.65), emotional intelligence (ESr=.58), and self-efficacy (ESr=.54). The characteristics of nursing competency (ESr=.45) also had a medium effect size and the effect size was larger for professional self-concept (ESr=.60), social support (ESr=.58), and critical thinking (ESr=.56). This result shows that individual characteristics were highly correlated with self-leadership compared to the characteristics of nursing competency. @*Conclusion@#This study is significant in that it verified the effect of variables related to self-leadership and presented the direction and preliminary data for a follow-up study. It is necessary to include variables considering individual characteristics in the development of an educational program for improving self-leadership in the future.

3.
Article in English | WPRIM | ID: wpr-761730

ABSTRACT

Malarial infection induces tissue hypoxia in the host through destruction of red blood cells. Tissue hypoxia in malarial infection may increase the activity of HIF1α through an intracellular oxygen-sensing pathway. Activation of HIF1α may also induce vascular endothelial growth factor (VEGF) to trigger angiogenesis. To investigate whether malarial infection actually generates hypoxia-induced angiogenesis, we analyzed severity of hypoxia, the expression of hypoxia-related angiogenic factors, and numbers of blood vessels in various tissues infected with Plasmodium berghei. Infection in mice was performed by intraperitoneal injection of 2×10⁶ parasitized red blood cells. After infection, we studied parasitemia and survival. We analyzed hypoxia, numbers of blood vessels, and expression of hypoxia-related angiogenic factors including VEGF and HIF1α. We used Western blot, immunofluorescence, and immunohistochemistry to analyze various tissues from Plasmodium berghei-infected mice. In malaria-infected mice, parasitemia was increased over the duration of infection and directly associated with mortality rate. Expression of VEGF and HIF1α increased with the parasitemia in various tissues. Additionally, numbers of blood vessels significantly increased in each tissue type of the malaria-infected group compared to the uninfected control group. These results suggest that malarial infection in mice activates hypoxia-induced angiogenesis by stimulation of HIF1α and VEGF in various tissues.


Subject(s)
Angiogenesis Inducing Agents , Animals , Hypoxia , Blood Vessels , Blotting, Western , Erythrocytes , Fluorescent Antibody Technique , Immunohistochemistry , Injections, Intraperitoneal , Malaria , Mice , Mortality , Parasitemia , Plasmodium , Plasmodium berghei , Vascular Endothelial Growth Factor A
4.
Article in Korean | WPRIM | ID: wpr-758532

ABSTRACT

PURPOSE: This paper reports the results of a massage therapy on transcutaneous bilirubin (TcB) in the forehead and inguinal regions, feeding intake, urine amount, and defecation frequency for neonates with jaundice who are receiving phototherapy. METHODS: A non-equivalent control group, pre-post, quasi-experimental design was used. Participants were 33 neonates with jaundice admitted for phototherapy and were randomly assigned to one of two groups—an experimental group (n=16), which received 3 days of routine plus 15 minutes of massage three times a day, and a control group (n=17), which received the routine care only. Data were analyzed using χ²-test and Mann-Whitney U test with SPSS 21.0 Win program. RESULTS: On the second day of hospitalization, the experimental group showed significantly lower TcB in the inguinal region (Z=−2.12, p=0.017); on the third day, the experimental group showed significantly lower TcB in the inguinal region (Z=−2.31, p=0.011) and higher defecation frequency (Z=−2.22, p=0.013) compared with the control group. CONCLUSIONS: The study findings suggest that massage for jaundice neonates receiving phototherapy is an effective intervention to reduce TcB in the inguinal region and to increase defecation frequency.


Subject(s)
Bilirubin , Defecation , Forehead , Hospitalization , Humans , Infant , Infant, Newborn , Jaundice , Massage , Phototherapy
5.
Laboratory Animal Research ; : 160-165, 2018.
Article in English | WPRIM | ID: wpr-719078

ABSTRACT

Breast cancer remains the second leading cause of cancer death among woman, worldwide, despite advances in identifying novel targeted therapies and the development of treating strategies. Classification of clinical subtypes (ER+, PR+, HER2+, and TNBC (Triple-negative)) increases the complexity of breast cancers, which thus necessitates further investigation. Mouse models used in breast cancer research provide an essential approach to examine the mechanisms and genetic pathway in cancer progression and metastasis and to develop and evaluate clinical therapeutics. In this review, we summarize tumor transplantation models and genetically engineered mouse models (GEMMs) of breast cancer and their applications in the field of human breast cancer research and anti-cancer drug development. These models may help to improve the knowledge of underlying mechanisms and genetic pathways, as well as creating approaches for modeling clinical tumor subtypes, and developing innovative cancer therapy.


Subject(s)
Animals , Breast Neoplasms , Breast , Classification , Female , Humans , Mice , Neoplasm Metastasis
6.
Article in Korean | WPRIM | ID: wpr-713958

ABSTRACT

PURPOSE: This study aimed to estimate the effects of a health mentoring program on fasting blood sugar, total cholesterol, triglyceride, physical activity, self care behavior and social support changes among community-dwelling vulnerable elderly individuals with diabetes. METHODS: A non-equivalent control group pre-post-test design was used. Participants were 70 community-dwelling vulnerable elderly individuals with diabetes. They were assigned to the experimental (n=30) or comparative (n=30) or control group (n=28). The experimental group participated in the health mentoring program, while the comparative group participated in health education program, the control group did not participate in any program. Data analyses involved a chi-square test, Fisher's exact test, a generalized linear model, and the Bonferroni correction, using SPSS 23.0. RESULTS: Compared to the control group, the experimental and comparative groups showed a significant decrease in fasting blood sugar, total cholesterol, and triglyceride. Compared to the comparative and control groups, the experimental group showed significant improvement in self care behavior. However, there were no statistical differences in physical activity or social support among the three groups. CONCLUSION: These findings indicate that the health mentoring program is an effective intervention for community-dwelling vulnerable elderly individuals with diabetes. This program can be used as an efficient strategy for diabetes self-management within this population.


Subject(s)
Aged , Blood Glucose , Cholesterol , Diabetes Mellitus , Fasting , Health Education , Humans , Linear Models , Mentors , Motor Activity , Self Care , Statistics as Topic , Triglycerides , Vulnerable Populations
7.
Article in Korean | WPRIM | ID: wpr-60157

ABSTRACT

PURPOSE: The purpose of this study was to investigate the influence of social support, self - esteem, hope, and health conservation of the vulnerable elderly people with diabetes. METHODS: Participants were 100 vulnerable elderly people with diabetes living in D or K cities. Data collection was done through interviews from February to March, 2016. Social support was measured with the MOS-SSS (Medical Outcomes Study Social Support Survey), self-esteem with Self-Esteem Scale, hope with the Nowotny Hope Scale, and health conservation with the Sung's Health Conservation Scale. IBM SPSS 20.0 was used to analyze descriptive statistics, one-way ANOVA, independent t-test, Pearson correlation, and stepwise multiple regression. RESULTS: Factors affecting the health conservation of the vulnerable elderly people were social support, hope, education level and subjective health status. These factors explained 64.9% of the health conservation. CONCLUSION: It is necessary to construct a comprehensive nursing classification system for elderly people with diabetes in vulnerable class and to develop integrated health conservation program and nursing care as a new social support resource.


Subject(s)
Aged , Classification , Data Collection , Diagnostic Self Evaluation , Education , Hope , Humans , Nursing , Nursing Care , Self Concept , Vulnerable Populations
8.
Article in English | WPRIM | ID: wpr-131560

ABSTRACT

Sphingosylphosphorylcholine (SPC) is one of the bioactive phospholipids that has many cellular functions such as cell migration, adhesion, proliferation, angiogenesis, and Ca²⁺ signaling. Recent studies have reported that SPC induces invasion of breast cancer cells via matrix metalloproteinase-3 (MMP-3) secretion leading to WNT activation. Thrombospondin-1 (TSP-1) is a matricellular and calcium-binding protein that binds to a wide variety of integrin and non-integrin cell surface receptors. It regulates cell proliferation, migration, and apoptosis in inflammation, angiogenesis and neoplasia. TSP-1 promotes aggressive phenotype via epithelial mesenchymal transition (EMT). The relationship between SPC and TSP-1 is unclear. We found SPC induced EMT leading to mesenchymal morphology, decrease of E-cadherin expression and increases of N-cadherin and vimentin. SPC induced secretion of thrombospondin-1 (TSP-1) during SPC-induced EMT of various breast cancer cells. Gene silencing of TSP-1 suppressed SPC-induced EMT as well as migration and invasion of MCF10A cells. An extracellular signal-regulated kinase inhibitor, PD98059, significantly suppressed the secretion of TSP-1, expressions of N-cadherin and vimentin, and decrease of E-cadherin in MCF10A cells. ERK2 siRNA suppressed TSP-1 secretion and EMT. From online PROGgene V2, relapse free survival is low in patients having high TSP-1 expressed breast cancer. Taken together, we found that SPC induced EMT and TSP-1 secretion via ERK2 signaling pathway. These results suggests that SPC-induced TSP-1 might be a new target for suppression of metastasis of breast cancer cells.


Subject(s)
Apoptosis , Breast Neoplasms , Cadherins , Cell Movement , Cell Proliferation , Epithelial-Mesenchymal Transition , Gene Silencing , Humans , Inflammation , Neoplasm Metastasis , Phenotype , Phospholipids , Phosphotransferases , Receptors, Cell Surface , Recurrence , RNA, Small Interfering , Thrombospondin 1 , Vimentin
9.
Article in English | WPRIM | ID: wpr-131558

ABSTRACT

Sphingosylphosphorylcholine (SPC) is one of the bioactive phospholipids that has many cellular functions such as cell migration, adhesion, proliferation, angiogenesis, and Ca²⁺ signaling. Recent studies have reported that SPC induces invasion of breast cancer cells via matrix metalloproteinase-3 (MMP-3) secretion leading to WNT activation. Thrombospondin-1 (TSP-1) is a matricellular and calcium-binding protein that binds to a wide variety of integrin and non-integrin cell surface receptors. It regulates cell proliferation, migration, and apoptosis in inflammation, angiogenesis and neoplasia. TSP-1 promotes aggressive phenotype via epithelial mesenchymal transition (EMT). The relationship between SPC and TSP-1 is unclear. We found SPC induced EMT leading to mesenchymal morphology, decrease of E-cadherin expression and increases of N-cadherin and vimentin. SPC induced secretion of thrombospondin-1 (TSP-1) during SPC-induced EMT of various breast cancer cells. Gene silencing of TSP-1 suppressed SPC-induced EMT as well as migration and invasion of MCF10A cells. An extracellular signal-regulated kinase inhibitor, PD98059, significantly suppressed the secretion of TSP-1, expressions of N-cadherin and vimentin, and decrease of E-cadherin in MCF10A cells. ERK2 siRNA suppressed TSP-1 secretion and EMT. From online PROGgene V2, relapse free survival is low in patients having high TSP-1 expressed breast cancer. Taken together, we found that SPC induced EMT and TSP-1 secretion via ERK2 signaling pathway. These results suggests that SPC-induced TSP-1 might be a new target for suppression of metastasis of breast cancer cells.


Subject(s)
Apoptosis , Breast Neoplasms , Cadherins , Cell Movement , Cell Proliferation , Epithelial-Mesenchymal Transition , Gene Silencing , Humans , Inflammation , Neoplasm Metastasis , Phenotype , Phospholipids , Phosphotransferases , Receptors, Cell Surface , Recurrence , RNA, Small Interfering , Thrombospondin 1 , Vimentin
10.
Article in Korean | WPRIM | ID: wpr-153579

ABSTRACT

PURPOSE: This study was conducted to identify the factors influencing health promotion behaviors. METHODS: The subjects were 200 students sampled from the population that attend four universities in G and J city. Data were collected with a questionnaire and were analyzed by t-test, ANOVA, correlation, and stepwise multiple regression. RESULTS: The mean age of subjects was 21. Family function and self-efficacy were identified as determinants of health promotion behaviors, which explained 53% of the total variances of health promotion behavior. CONCLUSION: The findings provide evidence to help health assessment and activity related to the health promotion behavior of university students.


Subject(s)
Health Promotion , Humans
11.
Article in Korean | WPRIM | ID: wpr-32771

ABSTRACT

PURPOSE: The purpose of this study was to develop a breastfeeding empowerment program and to investigate the effects of the breastfeeding empowerment program on self-efficacy, adaptation and continuation of breastfeeding for primiparous women. METHODS: The 5 session breastfeeding empowerment program was developed and a non-equivalent control group non-synchronized quasi-experiment design was used. Fifty-five participants were assigned to either the experimental group (n=27) or the control group (n=28). Effects were tested using repeated measures ANOVA and χ²-test. RESULTS: Scores for self-efficacy, adaptation and continuation of breastfeeding of in the experimental group after program were significantly higher than 1 week, 4 weeks, 8 weeks scores in control group. CONCLUSION: The effects of the breastfeeding empowerment program for elevating self-efficacy, adaptation and continuation of breastfeeding in primiparous women were validated. Therefore, this program can be recommended for vigorous use in clinical practice.


Subject(s)
Breast Feeding , Female , Humans , Power, Psychological , Self Efficacy
12.
Article in English | WPRIM | ID: wpr-145676

ABSTRACT

BACKGROUND: We compared the efficacies of vildagliptin (50 mg twice daily) relative to pioglitazone (15 mg once daily) as an add-on treatment to metformin for reducing glycosylated hemoglobin (HbA1c) levels in Korean patients with type 2 diabetes. METHODS: The present study was a multicenter, randomized, active-controlled investigation comparing the effects of vildagliptin and pioglitazone in Korean patients receiving a stable dose of metformin but exhibiting inadequate glycemic control. Each patient underwent a 16-week treatment period with either vildagliptin or pioglitazone as an add-on treatment to metformin. RESULTS: The mean changes in HbA1c levels from baseline were -0.94% in the vildagliptin group and -0.6% in the pioglitazone group and the difference between the treatments was below the non-inferiority margin of 0.3%. The mean changes in postprandial plasma glucose (PPG) levels were -60.2 mg/dL in the vildagliptin group and -38.2 mg/dL in the pioglitazone group and these values significantly differed (P=0.040). There were significant decreases in the levels of total, low density lipoprotein, high density lipoprotein (HDL), and non-HDL cholesterol in the vildagliptin group but increases in the pioglitazone group. The mean change in body weight was -0.07 kg in the vildagliptin group and 0.69 kg in the pioglitazone group, which were also significantly different (P=0.002). CONCLUSION: As an add-on to metformin, the efficacy of vildagliptin for the improvement of glycemic control is not inferior to that of pioglitazone in Korean patients with type 2 diabetes. In addition, add-on treatment with vildagliptin had beneficial effects on PPG levels, lipid profiles, and body weight compared to pioglitazone.


Subject(s)
Blood Glucose , Body Weight , Cholesterol , Glycated Hemoglobin A , Humans , Lipoproteins , Metformin , Thiazolidinediones
13.
Article in English | WPRIM | ID: wpr-104381

ABSTRACT

Epithelial mesenchymal transition (EMT) is the first step in metastasis and implicated in the phenotype of cancer stem cells. Therefore, understanding and controlling EMT, are essential to the prevention and cure of metastasis. In the present study, we examined, by Western blot, reverse transcription polymerase chain reaction (RT-PCR), and confocal microscopy, the effects of cardamonin (CDN) on transforming growth factor-beta1 (TGF-beta1)-induced EMT of A549 lung adenocarcinoma cell lines. TGF-beta1 induced expression of N-cadherin and decreased expression of E-cadherin. CDN suppressed N-cadherin expression and restored E-cadherin expression. Further, TGF-beta1 induced migration and invasion of A549 cancer cells, which was suppressed by CDN. TGF-beta1 induced c-Jun N-terminal kinase (JNK) activation during EMT, but CDN blocked it. Protein serine/threonine phosphatase 2A (PP2A) expression in A549 cancer cells was reduced by TGF-beta1 but CDN restored it. The overall data suggested that CDN suppresses TGF-beta1-induced EMT via PP2A restoration, making it a potential new drug candidate that controls metastasis.


Subject(s)
Adenocarcinoma , Blotting, Western , Cadherins , Cell Line , Epithelial-Mesenchymal Transition , JNK Mitogen-Activated Protein Kinases , Lung , Microscopy, Confocal , Neoplasm Metastasis , Neoplastic Stem Cells , Phenotype , Polymerase Chain Reaction , Protein Phosphatase 2 , Reverse Transcription , Transforming Growth Factor beta1
14.
Article in English | WPRIM | ID: wpr-225154

ABSTRACT

In Trichinella spiralis infection, type 2 helper T (Th2) cell-related and regulatory T (T(reg)) cell-related immune responses are the most important immune events. In order to clarify which Toll-like receptors (TLRs) are closely associated with these responses, we analyzed the expression of mouse TLR genes in the small intestine and muscle tissue during T. spiralis infection. In addition, the expression of several chemokine- and cytokine-encoding genes, which are related to Th2 and T(reg) cell mediated immune responses, were analyzed in mouse embryonic fibroblasts (MEFs) isolated from myeloid differentiation factor 88 (MyD88)/TIR-associated proteins (TIRAP) and Toll receptor-associated activator of interferons (TRIF) adapter protein deficient and wild type (WT) mice. The results showed significantly increased TLR4 and TLR9 gene expression in the small intestine after 2 weeks of T. spiralis infection. In the muscle, TLR1, TLR2, TLR5, and TLR9 gene expression significantly increased after 4 weeks of infection. Only the expression of the TLR4 and TLR9 genes was significantly elevated in WT MEF cells after treatment with excretory-secretory (ES) proteins. Gene expression for Th2 chemokine genes were highly enhanced by ES proteins in WT MEF cells, while this elevation was slightly reduced in MyD88/TIRAP-/- MEF cells, and quite substantially decreased in TRIF-/- MEF cells. In contrast, IL-10 and TGF-beta expression levels were not elevated in MyD88/TIRAP-/- MEF cells. In conclusion, we suggest that TLR4 and TLR9 might be closely linked to Th2 cell and T(reg) cell mediated immune responses, although additional data are needed to convincingly prove this observation.


Subject(s)
Animals , Gene Expression , Humans , Interleukin-10/genetics , Mice , Mice, Knockout , Th2 Cells/metabolism , Toll-Like Receptors/genetics , Trichinella spiralis/genetics , Trichinellosis/genetics
15.
Article in English | WPRIM | ID: wpr-225144

ABSTRACT

To determine whether pinworm infections and head lice infestations spread among children in orphanages, 117 children from 4 orphanages in Busan-si and Ulsan-si, Korea, were examined for enterobiasis and head lice infestation between January and February 2014. The overall rate of Enterobius vermicularis egg positivity was 0.85%, whereas none of the children had head lice infestations. The rate of pinworm infection was much lower among the orphanage children compared to the rates observed in previous studies among kindergarten and primary school students. Moreover, the risk factors for enterobiasis were less frequent among these subjects than previously reported. The personal hygiene and health of the orphanage children were supervised by a regular, employed nurse through a health education program. In conclusion, pinworm infection was efficiently controlled among the children in orphanages, and this might be related to good personal hygiene practices in Korea.


Subject(s)
Adolescent , Animals , Child , Child, Preschool , Enterobiasis/epidemiology , Enterobius/genetics , Female , Humans , Hygiene , Lice Infestations/epidemiology , Male , Orphanages/statistics & numerical data , Pediculus/genetics , Prevalence , Republic of Korea/epidemiology
16.
Article in English | WPRIM | ID: wpr-228916

ABSTRACT

The stiffness of cancer cells is attributable to intermediate filaments such as keratin. Perinuclear reorganization via phosphorylation of specific serine residue in keratin is implicated in the deformability of metastatic cancer cells including the human pancreatic carcinoma cell line (PANC-1). 12-O-Tetradecanoylphorbol-13-acetate (TPA) is a potent tumor promoter and protein kinase C (PKC) activator. However, its effects on phosphorylation and reorganization of keratin 8 (K8) are not well known. Therefore, we examined the underlying mechanism and effect of TPA on K8 phosphorylation and reorganization. TPA induced phosphorylation and reorganization of K8 and transglutaminase-2 (Tgase-2) expression in a time- and dose-dependent manner in PANC-1 cells. These effects peaked after 45 min and 100 nM of TPA treatment. We next investigated, using cystamine (CTM), Tgase inhibitor, and Tgase-2 gene silencing, Tgase-2's possible involvement in TPA-induced K8 phosphorylation and reorganization. We found that Tgase-2 gene silencing inhibited K8 phosphorylation and reorganization in PANC-1 cells. Tgase-2 gene silencing, we additionally discovered, suppressed TPA-induced migration of PANC-1 cells and Tgase-2 overexpression induced migration of PANC-1 cells. Overall, these results suggested that TPA induced K8 phosphorylation and reorganization via Tgase-2 expression in PANC-1 cells.


Subject(s)
Cell Line , Cystamine , Gene Silencing , Humans , Intermediate Filaments , Keratin-8 , Phosphorylation , Protein Kinase C , Serine
17.
Article in English | WPRIM | ID: wpr-87907

ABSTRACT

Skin hyperpigmentation is one of the most common skin disorders caused by abnormal melanogenesis. The mechanism and key factors at play are not fully understood. Previous reports have indicated that cystamine (CTM) inhibits melanin synthesis, though its molecular mechanism in melanogenesis remains unclear. In the present study, we investigated the effect of CTM on melanin production using ELISA reader and the expression of proteins involved in melanogenesis by Western blotting, and examined the involvement of transglutaminase-2 (Tgase-2) in SK-MEL-2 human melanoma cells by gene silencing. In the results, CTM dose-dependently suppressed melanin production and dendrite extension in alpha-MSH-induced melanogenesis of SK-MEL-2 human melanoma cells. CTM also suppressed alpha-MSH-induced chemotactic migration as well as the expressions of melanogenesis factors TRP-1, TRP-2 and MITF in alpha-MSH-treated SK-MEL-2 cells. Meanwhile, gene silencing of Tgase-2 suppressed dendrite extension and the expressions of TRP-1 and TRP-2 in alpha-MSH-treated SK-MEL-2 cells. Overall, these findings suggested that CTM suppresses alpha-MSH-induced melanogenesis via Tgase-2 inhibition and that therefore, Tgase-2 might be a new target in hyperpigmentation disorder therapy.


Subject(s)
Blotting, Western , Cystamine , Dendrites , Enzyme-Linked Immunosorbent Assay , Gene Silencing , Humans , Hyperpigmentation , Melanins , Melanoma , Skin
18.
Article in Korean | WPRIM | ID: wpr-85949

ABSTRACT

PURPOSE: This research compares self-leadership, team efficacy, problem solving processes and task satisfaction in response to teaching methods applied to nursing students, and determines whether variations exist. METHODS: This research experiments before and after the training of a nonequivalent group. The subjects were 36 learners of action learning methods and 39 learners of nursing course methods, and the research took place from October through December 2012. RESULTS: Prior to the training, the general features and measurable variables of the two groups of subjects were similar, and self-leadership, team efficacy, problem solving process and task satisfaction in both groups were elevated compared to pre-training. In particular, in comparison with the nursing course, there was a notable difference in scores, the action learning method receiving high scores in the problem solving process (t=2.92, p=.005) and task satisfaction (t=2.54, p=.013) CONCLUSION: It is recommended that educators not only conduct the practice training course for teaching methods, but also incorporate action learning.


Subject(s)
Humans , Leadership , Learning , Nursing , Problem Solving , Students, Nursing , Teaching
19.
Article in English | WPRIM | ID: wpr-185389

ABSTRACT

The high mortality rates associated with cancer reflect the metastatic spread of tumor cells from the site of their origin. Metastasis, in fact, is the cause of 90% of cancer deaths. Therefore, considerable effort is being made to inhibit metastasis. In the present study, we screened ketotifen for anti-migratory and anti-invasive activities against MDA-MB-231 breast cancer and HT-1080 fibrosarcoma cancer cells. Cancer cell migration and invasion were measured using multi-well chambers. Additionally, western blots were used to examine the effects of ketotifen on the expressions of CDC42, Rho, Rac, and matrix metalloproteinase 9 (MMP-9). The results showed that ketotifen dose-dependently suppressed the migration and invasion of MDA-MB-231 and HT-1080 cells. Ketotifen also suppressed the expressions of CDC42, Rac, and Rho, which, significantly, are involved in MDA-MB-231 and HT-1080 cancer cell migration. Moreover, ketotifen suppressed the expression and activity of MMP-9, which is involved in degradation of the extracellular matrix leading to invasion. The overall data suggested that ketotifen suppresses the migration and invasion of MDA-MB-231 and HT-1080 cancer cells via inhibition of CDC42, Rac, Rho, and MMP-9 expression.


Subject(s)
Blotting, Western , Breast Neoplasms , Cell Movement , Extracellular Matrix , Fibrosarcoma , Ketotifen , Matrix Metalloproteinase 9 , Mortality , Neoplasm Metastasis
20.
Korean Journal of Medicine ; : 603-608, 2014.
Article in Korean | WPRIM | ID: wpr-140475

ABSTRACT

Von Hippel-Lindau (VHL) disease is an inherited, autosomal dominant syndrome that manifests as a variety of benign and malignant tumors in various organs. Notably, renal cell carcinoma (RCC) in patients with VHL disease tends to be multifocal and bilateral, and tends to recur or new tumors develop after removal. Radiofrequency ablation (RFA) has recently been introduced as a minimally invasive treatment for small hereditary RCCs with a low complication rate and a minimal decrease in renal function. We present a case of successful RFA of multiple bilateral RCCs in a 24-year-old female with VHL. Computed tomography (CT) of the abdomen revealed multiple bilateral RCCs. The patient received sequential RFA for the bilateral RCCs, resulting in a complete response. At the time of writing, there was no sign of recurrence or renal failure. In conclusion, percutaneous RFA is a promising treatment for VHL patients who cannot undergo surgery, with excellent treatment outcomes and minimal change in renal function.


Subject(s)
Abdomen , Carcinoma, Renal Cell , Catheter Ablation , Female , Humans , Recurrence , Renal Insufficiency , von Hippel-Lindau Disease , Writing , Young Adult
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