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1.
Acta Pharmaceutica Sinica ; (12): 159-168, 2022.
Article in Chinese | WPRIM | ID: wpr-913182

ABSTRACT

The neonatal Fc receptor (FcRn) was first found to be a membrane protein that maternal antibodies transmitted to fetuses and newborns, and also expressed in multiple organs and tissues for whole life in adults. It plays a significant role to central regulate the lifespan of immunoglobulin G and serum albumin, as well as its involvement in innate and adaptive immune responses. In modern biopharmaceuticals, FcRn is a great potential drug delivery target and a highlighted subject for current research. This paper briefly describes the basic biological properties and action mechanism of FcRn, as well as the commonly used drug carrier design strategies of FcRn, especially the functional applications of prolonging half-life, targeted drug delivery, transmembrane and antigen presentation and so on. We propose that these distribution in different tissues and the diverse biological activities may have significant implications of targeting FcRn for novel drug delivery systems and immunotherapy.

2.
Article in Chinese | WPRIM | ID: wpr-908297

ABSTRACT

Objective:To explore the application effect of intelligent network-management system in the management of electronic controlled analgesic pump.Methods:A total of 70 medical staff were selected in Department of Anesthesiology from Peking University People′s Hospital from June to August 2018 and March to June 2019. Self controlled design was adopted. The data of 70 doctors and nurses were collected by comparing the development of electronic controlled analgesia pump before and after the two management modes in analgesia pump management of time-consuming and care satisfaction results.Results:Before the application of intelligent management system in the electronic controlled analgesia pump , the average access time of the analgesia pump was (3.725±0.332) min, and the satisfaction rate was 64.29% (45/70); after the application of the intelligent management system, the average access time of the analgesic pump was (1.901±0.237) min, and the satisfaction rate of the nurses was 97.14% (68/70). The difference before and after the application was statistically significant ( t value was 46.197, χ2 value was 8.425, P<0.05). Conclusions:Application of intelligent management system in the process of electronic controlled analgesia pump can effectively shorten the average access time of the analgesia pump, increase satisfaction rate, improve the work efficiency of clinical medical staff, and realize the network management of anesthesia analgesia pump.

3.
Article in Chinese | WPRIM | ID: wpr-907592

ABSTRACT

Lung cancer has the highest mortality rate among malignant tumors, and drug resistance in lung cancer treatment is currently a major challenge for clinical practitioners. Recent studies have found that epigenetics is closely linked to drug resistance in lung cancer, especially in DNA methylation, histone modifications and non-coding RNA regulation. By exploring the mechanisms affecting drug resistance in lung cancer through the above three features and exploring drugs that can effectively improve drug resistance in lung cancer in response to the mechanisms, it can provide ideas for solving the problems related to drug resistance in lung cancer in clinical work and provide a reference basis for prognostic assessment of lung cancer patients.

4.
Article in Chinese | WPRIM | ID: wpr-907585

ABSTRACT

Objective:To explore the effects of Ophiopogon D combined with cyclooxygenase-2 (COX-2) gene silencing on the proliferation, migration and invasion of human pancreatic cancer BxPC-3 cells.Methods:BxPC-3 cells were divided into blank control group, Ophiopogonin D high-dose group (40 μmol/L), medium-dose group (20 μmol/L) and low-dose group (10 μmol/L). The COX-2-slienced cells were divided into control group, COX-2 inhibited group (50 pmol/ml siRNA-COX-2), Ophiopogonin D group (20 μmol/L) and combination treatment group (Ophiopogonin D 20 μmol/L+ 50 pmol/ml siRNA-COX-2). The proliferation activity of BxPC-3 cells was detected by CCK-8, and the migration distance of BxPC-3 cells was detected by scratched assay. The invasion degree of BxPC-3 cells was detected by Transwell, the relative expression level of COX-2 gene in BxPC-3 cells was detected by real-time quantitative PCR (RT-qPCR), and the relative expressions of COX-2, hypoxia inducible factor-1α (HIF-1α) and vascular endothelial growth factor (VEGF) proteins in BxPC-3 cells were detected by Western blotting.Results:The cell proliferation rates of blank control group, Ophiopogonin D high-dose, medium-dose and low-dose groups were (100.0±4.9)%, (71.8±5.4)%, (80.5±5.8)% and (89.7±5.7)%, respectively. The migration distances were (279.8±24.0) μm, (141.9±21.2) μm, (168.8±37.1) μm and (224.6±19.9) μm, respectively. The absorbance ( A) values of invasion number were 1.107±0.095, 0.390±0.030, 0.596±0.017 and 0.826±0.034, respectively.There were statistically significant differences ( F=19.770, P<0.001; F=48.270, P<0.001; F=198.400, P<0.001). The above indexes of the Ophiopogonin D high-, medium- and low-dose groups were significantly lower than those in the blank control group (all P<0.05). The relative expression levels of COX-2 gene were 1.007±0.178, 0.387±0.169, 0.567±0.142 and 0.740±0.030, respectively, and the relative protein expression levels were 1.000±0.033, 0.654±0.085, 0.762±0.110 and 0.881±0.049, respectively, with statistically significant differences ( F=10.280, P=0.004; F=11.780, P=0.003). The above indexes of the Ophiopogonin D high- and medium-dose groups were significantly lower than those in the blank control group (all P<0.05), and there was no statistically significant difference between the Ophiopogonin D low-dose group and blank control group (both P>0.05). The medium-dose of Ophiopogonin D (20 μmol/L) was selected as the subsequent concentration.After COX-2 silencing, the proliferation rates of the control group, COX-2 inhibited group, Ophiopogonin D group and combination treatment group were (100.0±2.8)%, (68.4±6.7)%, (67.7±5.9)% and (57.0±8.5)%, respectively, the migration distances were (274.4±23.8) μm, (217.0±18.8) μm, (186.2±18.6) μm and (115.7±15.8) μm, respectively, and the A values of invasion number were 1.143±0.092, 0.791±0.058, 0.715±0.026 and 0.424±0.058, respectively, with statistically significant differences ( F=34.430, P<0.001; F=103.400, P<0.001; F=131.100, P<0.001). The proliferation rates, migration distances and invasion numbers in each treatment group were significantly lower than those in the control group (all P<0.001). Compared with the COX-2 inhibited group and Ophiopogonin D group, the cell proliferation, migration and invasion were significantly inhibited in the combination treatment group (all P<0.05). Compared with the Ophiopogonin D group, only the migration distance of the COX-2 inhibited group was significantly different ( P<0.05). The relative expression levels of COX-2 protein in the above groups were 0.995±0.037, 0.779±0.060, 0.806±0.076 and 0.645±0.079, respectively, the relative expression levels of HIF-1α were 1.083±0.104, 0.749±0.070, 0.736±0.070 and 0.394±0.016, respectively, and the relative expression levels of VEGF protein were 1.016±0.103, 0.757±0.090, 0.745±0.021 and 0.603±0.023, respectively, with statistically significant differences ( F=14.650, P=0.001; F=45.220, P<0.001; F=18.180, P<0.001). The expression levels of the three proteins in each treatment group were significantly lower than those in the control group (all P<0.05). Compared with the COX-2 inhibited group and Ophiopogonin D group, the relative protein expression levels of COX-2, HIF-1α and VEGF in the combination treatment group were significantly decreased (all P<0.05). Compared with the Ophiopogonin D group, there were no significant differences in the expression of the three proteins in the COX-2 inhibited group (all P>0.05). Conclusion:Ophiopogon D combined with COX-2 gene silencing can inhibit the proliferation, migration and invasion of pancreatic cancer cells, and the mechanism may be related to the inhibition of COX-2 pathway and the decrease of HIF-1α and VEGF protein expression levels.

5.
Article in Chinese | WPRIM | ID: wpr-884328

ABSTRACT

Objective:To summarize the prenatal sonographic characteristics of affected joints of fetal arthrogryposis multiplex congenita (AMC) by comparing the ultrasonographic features and the postnatal pathological manifestations.Methods:The cases of AMC detected by antenatal ultrasound and confirmed by postnatal pathology were collected in the First Affiliated Hospital of Sun Yat-Sen University and Kaiping Central Hospital from January 2015 to June 2020. The differences between prenatal ultrasonic manifestations, types of affected joints and postnatal pathological features were analyzed. And the different involvements of joints in AMC cases with or without other system abnormalities were also explored separately.Results:A total of 31 cases of AMC were included, in which 11 cases were with other system abnormalities and 20 cases without. No significant difference was observed in number of affected joints between these two groups ( P>0.05). The prenatal sonogram features were completely consistent with the postnatal pathological manifestations in 21 (21/31, 67.7%) cases. Among 31 cases, the involvement rates of joints were: interphalangeal joints of fingers (23/31, 74.2%), knee joints (20/31, 64.5%) and ankle joints (19/31, 61.3%), temporomandibular joint (11/31, 35.5%), wrists (11/31, 35.5%), elbow joints (10/31, 32.3%), interphalangeal joints of toes (6/31, 19.4%), spinal joints (2/31, 6.5%), shoulder joint (1/31, 3.2%) and hip joint (1/31, 3.2%), respectively. The coincidence rates of prenatal ultrasound in involved joints were: interphalangeal joints of fingers (100%), ankles (100%), spines (100%), hips (100%), wrists (90.9%), knees (75.0%), elbows (70.0%), jaws (54.5%), interphalangeal joints of toes (50.0%), and shoulders (0), respectively. Conclusions:When postural abnormalities of fetal upper and lower extremities are detected by prenatal ultrasound screening, especially overlapping fingers, extended knee and club foot, AMC should be kept on alert. Simultaneously, other joints should be carefully scanned to improve the prenatal detection rate of AMC.

6.
Acta Physiologica Sinica ; (6): 295-305, 2021.
Article in Chinese | WPRIM | ID: wpr-878258

ABSTRACT

Cortical GABAergic inhibitory neurons are composed of three major classes, each expressing parvalbumin (PV), somatostatin (SOM) and 5-hydroxytryptamine receptor 3A (Htr3a), respectively. Htr3a


Subject(s)
Animals , Interneurons/metabolism , Mice , Neurons/metabolism , Parvalbumins/metabolism , Receptors, Serotonin, 5-HT3/genetics , Serotonin , Somatostatin/metabolism
7.
Acta Physiologica Sinica ; (6): 1-9, 2021.
Article in Chinese | WPRIM | ID: wpr-878229

ABSTRACT

Astrocytes are a heterogenous group of macroglia present in all regions of the brain and play critical roles in many aspects of brain development, function and disease. Previous studies suggest that the B-cell lymphoma-2 associated X protein (BAX)-dependent apoptosis plays essential roles in regulating neuronal number and achieving optimal excitation/inhibition ratio. The aim of the present paper was to study whether BAX regulates astrocyte distribution in a region-specific manner. Immunofluorescence staining of SOX9 was used to analyze and compare astrocyte density in primary somatosensory cortex, motor cortex, retrosplenial cortex and hippocampus in heterozygous and homozygous BAX knockout mice at age of six weeks when cortical development has finished and glia development has reached a relatively steady state. The results showed that astrocyte density varied significantly among different cortical subdivisions and between cortex and hippocampus. In contrast to the significant increase in GABAergic interneurons, the overall and region-specific astrocyte density remained unchanged in the cortex when BAX was absent. Interestingly, a significant reduction of astrocyte density was observed in the hippocampus of BAX knockout mice. These data suggest that BAX differentially regulates neurons and astrocytes in cortex as well as astrocytes in different brain regions during development. This study provided important information about the regional heterogeneity of astrocyte distribution and the potential contribution of BAX gene during development.


Subject(s)
Animals , Astrocytes , Hippocampus , Interneurons , Mice , Neurons , bcl-2-Associated X Protein/genetics
8.
Journal of Chinese Physician ; (12): 525-528,534, 2020.
Article in Chinese | WPRIM | ID: wpr-867279

ABSTRACT

Objective:To investigate the protective effect and mechanism of total flavonoids of bidens bipinnata L (TFB) on vascular endothelial cell injury in children with henoch-schonlein purpura (HSP).Methods:Human umbilical vein endothelial cells (HUVECs) were cultured in vitro and intervened with normal and HSP children's serum. Then the HUVECs were divided into blank group, normal group, HSP group and TFB group. Levels of interleukin-8 (IL-8) and tumor necrosis factor α (TNF-α) in the supernatant were detected by enzyme-linked immunosorbent assay ( ELISA). The mRNA and protein expression of nuclear factor kappa B (NF-κB) and Fractalkine were detected by reverse polymerase chain reaction (RT-PCR) and Western blot in HUVECs. Results:ELISA results showed that the expression of IL-8 and TNF-α in HSP group were significantly higher than that in normal control group and blank group ( P<0.05), and the level of IL-8 and TNF-α in the TFB group was significantly lower than that in the HSP group ( P<0.05); the results of RT-PCR and Western blot showed that the expression levels of NF-κB, Fractalkine mRNA and protein in HSP group were significantly higher than those in normal group and blank group ( P<0.05). The expression levels of NF-κB, Fractalkine mRNA and protein in the TFB group were significantly lower than those in the HSP group ( P<0.05). Conclusions:TFB can inhibit the expression of NF-κB in children with Henoch-Schonlein purpura, reduce the secretion of IL-8 and TNF-α in HUVECs, and then relieve the occurrence of vascular injury.

9.
China Pharmacy ; (12): 35-41, 2020.
Article in Chinese | WPRIM | ID: wpr-817375

ABSTRACT

ABSTRACT OBJECTIVE:To study the improvement effect of Periplaneta americana extract Ento-A on damp-heat ulcerative colitis(UC)model rats. METHODS:Totally 70 rats were randomly divided into normal control group(n=8)and modeling group (n=62). The damp-heat UC model was induced in modeling group by high sugar,high fat,spicy diet combined with 2,4, 6-trinitrobenzene sulfonic acid enema. 48 modelrats were randomly divided into model control group,mesalazine group (300 mg/kg),Changyanning group(300 mg/kg)and Ento-A low-dose,medium-dose and high-dose(50,100,200 mg/kg,calculated by the extract),with 8 rats in each group. Normal control group and model control group were given normal saline intrsgastrically,and other groups were given relevant medicine intragastrically,once a day,for consecutive 14 days. After last administration, disease activity index (DAI), colonic mucosal injury index (CMDI) and histopathological score (HS)of rats were determined. The spleen index,liver index and colon index in rats were determined. The serum levels of IL-8,IL-17,SOD and MDA,colonic levels of IL-2,PGE2 and MPO were detected by ELISA. RESULTS:Compared with normal control group,the DAI score,CMDI score,HS score,colonic index,the serum levels of IL-8,IL-17 and MDA, colonic levels of MPO and PGE2 were increased significantly(P<0.01);serum level of SOD and colonic level of IL-2 were decreased significantly (P<0.01). Compared with model control group,DAI score,CMDI score,serum levels of IL-17 and MDA,colonic levels of PGE2 were decreased significantly in Ento-A high-dose groups(P<0.05 or P<0.01),while serum level of SOD and colonic level of IL-2 were increased significantly(P<0.01). CMDI score and HS score,serum levels of IL-8,IL-17 and MDA,colonic levels of PGE2 and MPO were decreased significantly in Ento-A medium-dose group(P<0.05 or P<0.01), while colonic level of IL-2 was increased significantly(P<0.01). HS score,serum levels of IL-17 and MDA,colonic levels of MPO and PGE2 were decreased significantly in Ento-A low-dose group(P<0.05 or P<0.01),while serum level of IL-2 was increased significantly(P<0.01). CONCLUSIONS:P. americana extract Ento-A may play improvement effect on damp-heat UC rats by regulating immune system balance and reducing inflammatory damage.

10.
China Pharmacy ; (12): 401-407, 2020.
Article in Chinese | WPRIM | ID: wpr-817283

ABSTRACT

OBJECTIVE:To investigate the effects of Periplaneta americana extract on the proliferation and apoptosis of human non-small cell lung cancer A 549 cells as well as its possible mechanism. METHODS :The dry bodies of P. americana were soaked with 90% ethanol and eluted with gradient water-methanol by polyamide column chromatography. The 20%,30%,40%, 50%,60%,70%,80%,90% methanol elution sites (YS-A-H)were obtained. MTT method was used to screen the active site , and the inhibition rate of different doses of active site was detected. Flow cytometry was adopted to detect cell apoptosis ,cell cycle and mitochondrial membrane potential of cells after treated with different doses of active site. RESULTS :Half inhibition concentrations of YS-A-H were (95.25±8.42),(129.93±7.24),(221.28±12.68),(275.39±14.87),(276.76±16.32),(31.90± 5.34),(163.15±6.97),(122.81±8.36)μg/mL,respectively. YS-F had the strongest activity. After treated with 3,9,27,81 μg/mL YS-F for 24,48,72 h,cell proliferation inhibitory rate was increased significantly at different time points ;after treated for 48,72 h,that was significantly higher than same group after treated for 24 h;after 72 h treatment ,that was significantly higher than same group after 48 h treatment (P<0.01). There was no significant effect of 24 h treatment of 3 μg/mL YS-F and 72 h treatment of 9 μg/mL YS-F on the percentage of cells in the late stage of necrosis,24 h treatment of 3 μg/mL YS-F on the percentage of cells in G2/M phase and 48 h treatment of 3 μg/mL YS-F on the reduction rate of mitochondrial membrane potential(P>0.05). The percentage of cells in the early stage of apoptosis ,the late stage of apoptosis and the early stage of necrosis ,the late stage of necrosis,as well as the percentage of cells in the Sub-G 0/G1 and S phase at each time point were significantly increased in other different doses groups ,while the percentage of cells in G 0/G1 and G 2/M phase was decreased significantly (P<0.01). In each dose group,the percentage of cells in the early stage of apoptosis ,the late stage of apoptosis and the early stage of necrosis ,the late stage of necrosis (except for the percentage of cells in the late stage of necrosis treated with YS-F 9 μg/mL for 72 h)and the percentage of cells in Sub-G 0/G1 phase,G2/M phase (except for YS-F 27,81 μg/mL for 48 h)after treated for 48,72 h were significantly higher than same group after 24 h of treatment ;the percentage of cells in G 0/G1 phase,S phase and G 2/M phase (except for YS-F 9 μg/mL for 48 h)after treated for 48,72 h were significantly lower than same group after 24 h of treatment (P<0.01);the percentage of cells in the early stage of apoptosis ,the late stage of apoptosis and the early stage of necrosis ,the late stage of necrosis (except for the percentage of cells in the late stage of apoptosis and early stage of necrosis when treated with YS-F 27 μg/mL for 72 h,the percentage of cells in the late stage of necrosis when treated with YS-F 3,9 μg/mL for 72 h were decreased significantly )and the percentage of cells in S phase (except for YS-F 3 μg/mL for 72 h)and Sub-G 0/G1 phase after treated for 72 h were significantly higher than same group after 48 h of treatment ,while the percentage of cells in G 0/G1 and G 2/M phase were significantly lower than same group after 48 h of treatment (P<0.01). After treated with YS-F 9,27,81 μg/mL for 48 h,the reduction rate of cell mitochondrial membrane potential was increased significantly ;YS-F 27,81 μg/mL groups were significantly higher than YS-F 9 μg/mL group,and YS-F 81 μg/mL group was significantly higher than YS-F 27 μg/mL group. CONCLUSIONS:YS-F can inhibit the proliferation and promote the apoptosis of A 549 cells by preventing cell transformation from S phase to G 2/M phase ,and reducing mitochondrial membrane potential ,in time-dependent or dose-dependent manner.

11.
Chinese Journal of Geriatrics ; (12): 435-438, 2020.
Article in Chinese | WPRIM | ID: wpr-869401

ABSTRACT

Objective:To investigate effects of early postoperative enteral nutrition in combination of micro-ecological feed preparations on intestinal flora and immune function in elderly colorectal cancer patients after surgery.Methods:Eighty-eight elderly colorectal cancer patients treated at our hospital from November 2017 to August 2019 were enrolled in the study.They were randomly divided into two groups(n=44, each): the observation group receiving micro-ecological feed preparations and enteral nutrition, and the control group receiving conventional parenteral nutrition.The intestinal flora index, cellular immune function, humoral immune function and complications were compared between the two groups.Results:There was no significant difference in the indices of intestinal flora between the two groups before operation(all P>0.05). In the control group, Bifidobacteria and Lactobacillus decreased, E. coli increased, and B/E value decreased after surgery compared with before surgery( P<0.05). In the observation group, Bifidobacteria and Lactobacillus decreased, E. coli increased and B/E value decreased after surgery compared with before surgery( P<0.05). Compared with the control group, the observation group showed that Bifidobacteria and Lactobacillus increased, E. coli decreased and B/E value increased after surgery( P<0.05). Serum inflammatory factors had no statistically significance difference between the two groups before operation( P>0.05). Levels of interleukin-6, C-reactive protein and tumor necrosis factor-α were increased in the two groups after surgery compared with before surgery( P<0.05), and the indexes were lower in the observation group than in the control group( t=4.841, 7.247 and 4.231, all P=0.000). Levels of IgG, IgM, IgA, CD3 + , CD4 + , CD8 + and the ratio of CD4 + and CD8 + were higher in the observation group than in the control group[(12.53±1.84) g/L vs.(11.47±1.92)g/L, (1.45±0.22) g/L vs.(1.33±0.17) g/L, (2.28±0.24) g/L vs.(2.13±0.12) g/L, (52.84±4.12)% vs.(50.43±3.91)% , (34.53±3.51)% vs.(30.75±3.62)%, (19.09±2.41)% vs.(18.24±0.53)% and (1.68±0.22)% vs.(1.43±0.15)%, t=2.644, 2.863, 3.120, 2.814, 4.973, 2.285 and 6.228, P=0.010, 0.005, 0.003, 0.006, 0.000, 0.025 and 0.000]. Conclusions:Early micro-ecological feed preparation in combination of enteral nutrition can improve the surgical effect and quality of life and promote disease recovery with a safety in patients with colorectal cancer.

12.
Asian Journal of Andrology ; (6): 590-601, 2020.
Article in English | WPRIM | ID: wpr-879702

ABSTRACT

The mammalian epididymis not only plays a fundamental role in the maturation of spermatozoa, but also provides protection against various stressors. The foremost among these is the threat posed by oxidative stress, which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids, proteins, and nucleic acids. In mice, the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5 (GPX5) as a major luminal scavenger in the proximal caput epididymidal segment. Accordingly, the loss of GPX5-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx5

13.
Chinese Journal of Endemiology ; (12): 302-305, 2019.
Article in Chinese | WPRIM | ID: wpr-744302

ABSTRACT

Objective To analyze the epidemic situation of brucellosis in Shenyang,reveal its epidemic characteristics,and provide scientific basis for prevention and control of brucellosis.Methods The epidemiological data of brucellosis in Shenyang in 2016 and 2017 were collected from the "Infectious Disease Reporting Information Management System" and the questionnaires of confirmed cases of brucellosis in Shenyang.Descriptive epidemiological method was used to analyze the "three-way distribution" of brucellosis and the time interval of case diagnosis,discovery agency and infection route.Results In 2016 and 2017,910 cases of brucellosis were reported in Shenyang,with an average incidence of 5.52/100 000.The incidence of brucellosis was mainly concentrated in March-August (627 cases),with the highest incidence in May (139 cases),accounting for 15.27%.There were brucellosis cases reported in 13 counties (cities and districts) of Shenyang,among which Xinmin City was the largest (341 cases),accounting for 37.47%;including 667 males and 243 females,with a sex ratio of 2.47:1.00;the 45-49 age group was the most (168 cases),accounting for 18.46%;the farmer was the most (555 cases),accounting for 60.99%.The average time interval of case diagnosis was 26 d,the shortest was 0 d and the longest was 252 d,and the confirmed case within 8-30 d was the most (432 cases),accounting for 47.47%.Totally 491 cases were found by Shenyang Infectious Diseases Hospital,accounting for 53.96%;the remaining 419 cases were found by hospitals at all levels,accounting for 46.04%.The main infection route was feeding and herding (616 cases),accounting for 67.69%.Conclusions There are obvious regional,seasonal,gender,age and occupational differences in the occurrence of brucellosis.Some cases of brucellosis are diagnosed for a long time.It is necessary to carry out brucellosis surveillance for key groups,carry out health education for occupational groups and improve the diagnostic level of brucellosis by primary clinicians.

14.
Article in Chinese | WPRIM | ID: wpr-754849

ABSTRACT

Objective To explore the prenatal ultrasound diagnosis and postnatal clinical outcomes of fetuses with hepatohilar cystic occupying lesions . Methods T his was a retrospective study that included all fetuses found to have hepatohilar cystic occupying lesions diagnosed by ultrasound in the First Affiliated Hospital of Sun Yat‐sen University between January 2008 and December 2017 . According to the morphology of the cyst and max diameter ,the cases were divided into four groups . Cases with polygonal cysts and max diameter over 30 mm were assigned to group 1 ,non‐polygonal cysts and max diameter over 30 mm to group 2 ,polygonal cysts and max diameter under 30 mm to group 3 ,non‐polygonal cysts and max diameter under 30 mm to group 4 . T he birth status ,ultrasound review image ,surgical treatment and pathological findings were tracked to analyze the prognosis of these fetuses . Results Among 47 cases of fetal hepatohilar cystic occupying lesions ,38 fetuses were born and 9 were terminated with only 1 case taking pathological examination . T here were 39 cases with pregnancy outcomes . T wenty‐seven cases ( 69 .2% ,27/39) were diagnosed as congenital biliary dilatation ( CBD) ,5 cases ( 12 .8% ,5/39) were found cysts resolved in postnatal ultrasound examinations . Biliary atresia was diagnosed in 3 cases ( 7 .8% ,3/39 ) by operation ; M esenteric cysts ( 5 .1% ,2/39) were diagnosed in 2 cases by ultrasound . One ( 2 .6% ,1/39) was diagnosed as double‐gallbladder by ultrasound . 1 ( 2 .6% , 1/39 ) was diagnosed as teratoma by operation . M ost cases were in the group 3 ,but there was no significant difference compared with other groups ( all P > 0 .05) . Conclusions More than half of hepatohilar cystic occupying lesions diagnosed in prenatal ultrasound are proved to be CBD with good prognosis . T he cysts in few cases can be resolved after born . 7 .8% of cases are biliary atresia with poor prognosis ,w hich give messages to prenatal clinical consultation .

15.
Journal of Chinese Physician ; (12): 1199-1204, 2019.
Article in Chinese | WPRIM | ID: wpr-754294

ABSTRACT

Objective To investigate the effects of budesonide (BUN) on high mobility group protein 1 (HMGB1)/toll-like receptor 4 (TLR4) / nuclear factor κB (NF-κB) pathway in neonatal mice with hyperoxia-induced bronchopulmonary dysplasia (BPD).Methods 80 mice were randomly divided into control group,model group,low,medium and high dose BUN groups,with 16 mice in each group.Mice in the control group were exposed to air,and mice in model group and BUN low,medium and high dose groups were exposed to 60% oxygen to establish BPD model;After 24 hours of modeling,the control group and model group were inhaled with normal saline every day.The low,middle and high dose BUN groups inhaled (1,2,4 ml) BUN,respectively,12 h/times,and continued to inhale until the mice were executed.On the 7th and 14th days of the model establishment,hematoxylin-eosin (HE) staining was used to observe the morphological changes of lung tissue;the levels of interleukin (IL)-1β,IL-6,tumor necrosis factor-α (TNF-α),malondialdehyde (MDA) and superoxide dismutase (SOD) in lung tissues were detected by enzyme-linked immunosorbent assay (ELISA);the levels of HMGB1,TLR4 and NF-κB in lung tissue were detected by real-time fluorescence quantitative polymerase chain reaction (qRT-PCR) and protein immunoblotting (WB).Results In the model group,the lung tissue structure was severely damaged and the alveolar volume was enlarged to form bullae on the 7th and 14th day.In low,middle and high dose groups,with the increase of BUN concentration,the lung tissue deformation gradually improved and the alveolar structure gradually became intact.Compared with the control group,the number of radial alveoli in the lung tissues of the model group decreased at 7th and 14th days;SOD activity decreased;the expression levels of IL-1β,IL-6,TNF-α,MDA,HMGB1,TLR4 and NF-κB mRNA and protein were increased (P <0.05).Compared with the model group,the number of radial alveoli and SOD activity in lung tissues of BUN groups increased gradually at 7th and 14th days,while the levels of IL-1β,IL-6,TNF-α,MDA,HMGB1,TLR4and NF-κB decreased gradually at different doses (P < 0.05).Conclusions BUN may inhibit the levels of inflammatory factors and oxidative stress by regulating HMGB1/TLR4/NF-κB pathway to achieve the protective effect on BPD neonatal mice.

16.
Chinese Journal of Anesthesiology ; (12): 1172-1175, 2019.
Article in Chinese | WPRIM | ID: wpr-824682

ABSTRACT

Objective To evaluate the effect of dexmedetomidine on cell apoptosis during lung is-chemia-reperfusion(I/R)injury in a rat model of cardiopulmonary bypass(CPB).Methods Ninety-six SPF healthy adult male Sprague-Dawley rats,weighing 350-500 g,were divided into 4 groups(n=24 each)using a random number table method: sham operation group(group S),CPB group(group C),CPB plus left lung I/R group(group IR),and CPB plus left lung I/R plus dexmedetomidine group(group D).The chest was only opened,and the rats underwent no CPB in group S.Only the CPB model was es-tablished in group C.The model of left lung I/R injury was established based on the CPB model in group IR.In group D,the model of CPB plus left pulmonary I/R injury was established,dexmedetomidine was intrave-nously infused in a dose of 3 μg/kg through the tail vein,followed by a continuous infusion of 1.5 μg?kg-1?h-1 until the end of surgery.Eight rats were selected in each group before operation(T0),at 10 min after opening the left hilum(T1),and at the end of operation(T2),the left lung tissues were taken for examination of pathological changes(with a light microscope)which were scored and for determination of cell apoptosis,and immunohistochemistry score(IHS)was assessed.The apoptosis index was calculated.Results Compared with group S,the pathological changes of lung tissues,IHS and apoptosis index were significantly increased at T1,2 in the other three groups(P<0.05).Compared with group C,the pathologi-cal changes of lung tissues,IHS and apoptosis index were significantly increased at T1,2 in IR and D groups(P<0.05).Compared with group IR,the pathological changes of lung tissues,IHS and apoptosis index were significantly decreased at T2 in group D(P<0.05).Conclusion The mechanism by which dexme-detomidine reduces lung I/R injury during CPB is related to inhibiting cell apoptosis in rats.

17.
Article in Chinese | WPRIM | ID: wpr-776770

ABSTRACT

OBJECTIVE@#To explore the influence of uniparental disomy (UPD) on bipartite and tripartite paternity testing.@*METHODS@#Two cases of paternity testing were analyzed by multiplex amplification and capillary electrophoresis typing. Suspected UPD was verified by using single nucleotide polymorphism array (SNP array). Parental power index was calculated by using a bipartite or tripartite model.@*RESULTS@#The two cases were found to harbor respectively three short tandem repeats on chromosome 2 and two short tandem repeats on chromosome 15. SNP array verified that both cases were of UPD. Case 1 had a parental power index of 122274987565.23 by a tripartite model, while case 2 had a parental power index of 13500.8463 by a bipartite model. Based on the technical specification, the conclusions supported a biological parent-child relationship in both cases.@*CONCLUSION@#UPD may lead to misjudgment of paternity testing. The possibility of UPD should be considered when certain loci which do not conform to Mendelian inheritance have aggregated to one chromosome.


Subject(s)
Chromosomes, Human, Pair 2 , Genetics , Humans , Microsatellite Repeats , Paternity , Polymorphism, Single Nucleotide , Uniparental Disomy , Genetics
18.
Article in Chinese | WPRIM | ID: wpr-775092

ABSTRACT

OBJECTIVE@#To examine the levels of airway inflammatory mediators in peripheral blood in infants and young children with wheezing and to study the possible pathogenesis of wheezing from the aspects of T helper cell 1 (Th1)/T helper cell 2 (Th2) imbalance and airway inflammation.@*METHODS@#A total of 50 children aged 1 month to 3 years with an acute wheezing episode were enrolled as the wheezing group, and 25 age-matched healthy infants were enrolled as the healthy control group. According to the number of wheezing episodes, the wheezing group was divided into a first-episode group (n=25) and a recurrent wheezing (number of episodes ≥2) group (n=25). According to the presence or absence of high-risk factors for asthma, the wheezing group was divided into a high-risk factor group (n=22) and a non-high-risk factor group (n=28). According to the results of pathogen detection, the wheezing group was divided into a positive pathogen group (n=23) and a negative pathogen group (n=27). Levels of interleukin-2 (IL-2), interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-13 (IL-13), transforming growth factor-β1 (TGF-β1), and total IgE (TIgE) in peripheral blood were measured for each group. For children with wheezing, eosinophil (EOS) count in peripheral blood was measured, and related samples were collected for respiratory pathogen detection.@*RESULTS@#The wheezing group had significantly higher levels of IL-4, IL-5, IL-13, TGF-β1, and TIgE in peripheral blood than the healthy control group (P0.05). The correlation analysis showed that in children with wheezing, EOS count was positively correlated with IL-4 level (P<0.01), IL-4 level was positively correlated with IL-5 and IL-13 levels (P<0.01), IL-5 level was positively correlated with IL-13 level (P<0.01), and IL-2 level was positively correlated with TGF-β1 level (P<0.05).@*CONCLUSIONS@#Th1/Th2 imbalance with a predominance of Th2 is observed in infants and young children with wheezing. IL-4, IL-5, IL-13, TGF-β1, and IgE are involved in the pathogenesis of wheezing in these children. Airway inflammation is also observed in these children with wheezing, but it is not associated with the number of wheezing episodes, presence or absence of high-risk factors for asthma, or results of pathogen detection.


Subject(s)
Asthma , Child , Child, Preschool , Humans , Infant , Inflammation Mediators , Interleukin-13 , Respiratory Sounds , Th1 Cells
19.
Article in Chinese | WPRIM | ID: wpr-775048

ABSTRACT

OBJECTIVE@#To investigate the current status of neglect in children aged 3-6 years in the rural areas of Urumqi, China and risk factors for neglect.@*METHODS@#Stratified random cluster sampling was performed to select 1 326 children, aged 3-6 years, from 6 kindergartens in the rural areas of Urumqi. A self-designed questionnaire and "Chinese Neglect Evaluation Questionnaire for Rural Children Aged 3-6 Years" were used for investigation.@*RESULTS@#Of the 1 326 children, the neglect rate was 51.89% (688 cases), and the degree of neglect was 49±6. There were significant differences in the neglect rate and the degree of neglect between the children in different age groups (P<0.01). The single-parent families and the remarried families had significantly higher child neglect rate and degree of neglect than the families of three generations under one roof and the core families (P<0.01). The children in left-behind status had a significantly higher degree of neglect (P<0.01). The multivariate logistic regression analysis showed that younger children, mothers with low education level, employment status of the mother as a migrant worker, single-parent and remarried families, low-income families, and left-behind status were risk factors for child neglect (P<0.05).@*CONCLUSIONS@#The neglect of children aged 3-6 years in the rural areas of Urumqi is serious, and more attention should be paid to younger children, children whose mothers with low education level, left-behind children, and children from single-parent families, remarried families and low-income families.


Subject(s)
Child , Child Abuse , Child, Preschool , China , Female , Humans , Mothers , Risk Factors , Rural Population , Surveys and Questionnaires
20.
Article in Chinese | WPRIM | ID: wpr-817777

ABSTRACT

@#【Objective】To investigate the clinical value of chromosomal microarray analysis(CMA)for fetuses with persistent left superior vena cava(PLSVC).【Methods】Fetuses that were diagnosed with PLSVC during ultrasound examination and underwent invasive prenatal testing(on which karyotyping and CMA were both performed)from January 2014 to December 2016 at the First Affiliated Hospital of Sun Yat-sen University were reviewed. According to the combination of other ultrasound abnormalities,the cases were divided into isolated group and complicated group.【Results】Karyotype analysis identified chromosomal aberrations in 18.5%(15/81)of the fetuses,while CMA detected pathogenic copy number variations(CNV)in 23.5%(19/81)of the fetuses. There was no significant difference in the detection rate of chromosomal abnormalities between the Karyotype analysis and CMA(P = 0.44). CMA achieved an incremental yield of 6.1% (4/66)among PLSVC fetuses with normal karyotypes,and only in the complicated cases. There were 12 cases(14.8% ,12/81)in isolated group and 69 cases(85.2% ,69/81)in complicated group. The frequency of genetic anomalies in the complicated group was not significantly higher than that in the isolated group(26.1%,18/69 vs. 8.3%,1/12,P = 0.277). The incidences of atrioventricular septal defect,facial abnormalities,and multiple soft markers were significantly higher among fetuses with abnormal genetic test results(P= 0.030,P= 0.012,P= 0.014).【Conclusion】CMA is a valuable tool for identifying additional unbalanced submicroscopic chromosomal abnormalities in fetuses with PLSVC ,especially when PLSVC is accompanied by other ultrasound malformations.

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