Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 128
Filter
1.
Article in English | WPRIM | ID: wpr-922533

ABSTRACT

BACKGROUND@#Coronavirus disease 2019 (COVID-19) has become a worldwide life-threatening pandemic. Lianhua Qingwen is believed to possess the ability to treat or significantly improve the symptoms of COVID-19. These claims make it important to systematically evaluate the effects of using Lianhua Qingwen with Western medicine to treat COVID-19.@*OBJECTIVE@#To evaluate the safety and efficacy of combination therapy, employing Lianhua Qingwen with Western medicine, to treat COVID-19, using a meta-analysis approach.@*SEARCH STRATEGY@#China National Knowledge Infrastructure, Wanfang Database, VIP Database, PubMed, Embase, and Cochrane Library databases were searched for studies evaluating the effect of Lianhua Qingwen-Western medicine combination therapy in the treatment of COVID-19.@*INCLUSION CRITERIA@#(1) Research object: hospitalized patients meeting the diagnostic criteria of COVID-19 were included. (2) Intervention measures: patients in the treatment group received Lianhua Qingwen treatment combined with Western medicine, while the control group received either Western medicine or Chinese medicine treatment. (3) Research type: randomized controlled trials and retrospective study were included.@*DATA EXTRACTION AND ANALYSIS@#Two researchers extracted the first author, the proportion of males and females, age, body temperature, course of treatment, rate of disappearance of main symptoms, duration of fever, adverse reactions, and total effectiveness from the literature. Odds ratio (OR) and 95% confidence interval (CI) were used as the effect value for count data, and mean difference (MD) and 95% CI were used as the effect value for measurement data.@*RESULTS@#Six articles met the inclusion criteria, including a total of 856 COVID-19 patients. The meta-analysis showed that Lianhua Qingwen combination therapy achieved higher rates of fever reduction (OR = 3.43, 95% CI [1.78, 6.59], P = 0.0002), cough reduction (OR = 3.39, 95% CI [1.85, 6.23], P < 0.0001), recovery from shortness of breath (OR = 10.62, 95% CI [3.71, 30.40], P < 0.0001) and recovery from fatigue (OR = 2.82, 95% CI [1.44, 5.53], P = 0.003), higher total effectiveness rate (OR = 2.51, 95% CI [1.73, 3.64], P < 0.00001), and shorter time to recovery from fever (MD = -1.00, 95% CI [-1.04, 0.96], P < 0.00001), and did not increase the adverse reaction rate (OR = 0.65, 95% CI [0.42, 1.01], P = 0.06), compared to the single medication control.@*CONCLUSION@#The Lianhua Qingwen and Western medicine combination therapy is highly effective for COVID-19 patients and has good clinical safety. As only a small number of studies and patients were included in this review, more high-quality, multicenter, large-sample-size, randomized, double-blind, controlled trials are still needed for verification.


Subject(s)
COVID-19 , Drugs, Chinese Herbal , Female , Humans , Male , Multicenter Studies as Topic , Pandemics , Randomized Controlled Trials as Topic , Retrospective Studies , SARS-CoV-2
2.
Chinese Journal of Biotechnology ; (12): 2425-2434, 2021.
Article in Chinese | WPRIM | ID: wpr-887808

ABSTRACT

Realtime xCELLigence analysis (RTCA) is a new cell detection technology to continuously monitor, record and analyze a variety of information generated by cell activity. In drug research, it plays an important role in assessing myocardial toxicity and cell biological activity. Here, we first introduce the underlying mechanisms and characteristics of RTCA. Then we review the applications of RTCA in the research of myocardial toxicity and cell biological activity, to provides the fundamental baseline for understanding and exploiting RTCA. With the real-time, unlabeled, non-invasive, high throughput, and high accuracy features, RTCA not only promotes drug research and development, but also has a broad and good application prospect in other fields.


Subject(s)
Pharmaceutical Preparations
3.
Article in Chinese | WPRIM | ID: wpr-882604

ABSTRACT

Plague, infectious disease in modern medicine, refers to a type of disease with strong pathogenicity and infectiousness, it refers to the infectious diseases of western medicine. Due to its wide variety, the knowledge and understanding of plagues of Traditional Chinese Medicine (TCM) doctors in different stages have evolved and developed with the times. This article, via collating ancient documents, differentiatesthe classification in TCM and analyzes itsrelated theories to perfect the type of plagues in TCM, providing the theoretical basis for the research of plague in modern times.

4.
China Pharmacy ; (12): 571-577, 2021.
Article in Chinese | WPRIM | ID: wpr-873671

ABSTRACT

OBJECTIVE:To investigate the activity of lycorine to the in vivo apoptosis of tumor cells in H 22-bearing mice and its mechanism. METHODS :Kunming mice were inoculated subcutaneously with ascites of H 22 hepatoma mice in the armpit of forelimb to establish solid tumor model. After modeling ,mice were randomly divided into negative control group ,positive control group(hydroxycamptothecin 6 mg/kg),lycorine low-dose ,medium-dose and high-dose groups (10,20,40 mg/kg),with 10 mice in each group. Negative control group was given constant volume of normal saline intragastrically ,and administration groups were given relevant medicine intragastrically ,once a day ,for consecutive 7 days. After last medication ,the weight of tumor was detected and anti-tumor rate was calculated. Ascites tumor model of mice was established by intraperitoneal injection of H 22 hepatoma mice ascites ,and then were grouped with same method and given relevant medicine as above. After last medication , survival time of mice was recorded and the life prolongation rate was calculated. The early apoptotic rate of tumor cells in mice was detected by flow cytometry. On the basis of normal control group (normal mice without tumor ),the mitochondrial membrane permeability of tumor cells in each group was investigated by Calcein AM staining. The changes of mitochondrial potential were investigated by Rhodamine 123 staining. Colorimetry and Western blot assay were adopted to detect the Caspase-3 activity and expression of apoptosis-related protein (Bcl-2,Bax,Cyt-C and Caspase- 9). RESULTS :Compared with negative control UN- group,the tumor weight of positive control group and lycorine PYSCT-2017208) groups were decreased significantly ,while the survival time was significantly prolonged ,and the early apoptotic rate of tumor cells was significantly increased (P<0.05 or P<0.01);the anti-tumor rates were 39.41% , 23.36% , 36.50% , 56.93%,and life prolonga tion rates were 49.23%,29.09%, E-mail:ym913@yahoo.com.cn 50.19%,69.08%. Compared with normal control group ,the mitochondrial membrane permeability ,Caspase-3 protein activity and protein expression of Cyt-C and Caspase- 9 were significantly increased,while the mitochondrial membrane potential and Bcl- 2/Bax ratio were decreased significantly (P<0.05 or P<0.01). Compared with negative control group ,mitochondrial membrane permeability and Bcl- 2/Bax ratio were decreased significantly in administration groups ,while mitochondrial permeability ,Caspase-3 protein activity and protein expression of Cyt-C and Caspase- 9 were significantly increased (P<0.05 or P<0.01). CONCLUSIONS :Lycorine can induce the apoptosis of tumor cells in H22-bearing mice ,the effects of which may be associated with opening mitochondrial membrane permeability transition pore to increase mitochondrial permeability , decreasing mitochondrial membrane potential and up-regulating the expression of apoptosis-related proteins.

5.
Chinese Medical Journal ; (24): 2589-2596, 2021.
Article in English | WPRIM | ID: wpr-921166

ABSTRACT

BACKGROUND@#Finding an optimal treatment strategy for adolescent idiopathic scoliosis (AIS) patients remains challenging because of its intrinsic complexity. For mild to moderate scoliosis patients with lower skeletal growth potential (Risser 3-5), most clinicians agree with observation treatment; however, the curve progression that occurs during puberty, the adolescent period, and even in adulthood, remains a challenging issue for clinicians. The aim of the study is to investigate the efficacy of Schroth exercise in AIS patients with lower skeletal growth potential (Risser 3-5) and moderate scoliosis (Cobb angle 20°-40°).@*METHODS@#From 2015 to 2017, data of 64 patients diagnosed with AIS in Peking University Third Hospital were reviewed. Forty-three patients underwent Schroth exercise were classified as Schroth group, and 21 patients underwent observation were classified as observation group. Outcomes were measured by health-related quality of life (HRQOL) and radiographic parameters. HRQOL was assessed using the visual analog scale (VAS) scores for back, Scoliosis Research Society-22 (SRS-22) patient questionnaire. Radiographic spinopelvic parameters were obtained from anteroposterior and lateral X-rays. The pre-treatment and post-treatment HRQOL and radiographic parameters were tested to validate Schroth exercise efficacy. The inter-rater reliability of the radiographic parameters was tested using the interclass correlation coefficient (ICC). The paired t test was used to examine HRQOL and radiographic parameters. Clinical relevance between C2-C7 sagittal vertical axis (SVA) and thoracic kyphosis was analyzed using Spearman correlation.@*RESULTS@#In Schroth group, VAS back score, SRS-22 pain, and SRS-22 self-image domain were significantly improved from pre-treatment 3.0 ± 0.8, 3.6 ± 0.5, and 3.5 ± 0.7 to post-treatment 1.6 ± 0.6 (t = 5.578, P = 0.013), 4.0 ± 0.3 (t = -3.918, P = 0.001), and 3.7 ± 0.4 (t = -6.468, P < 0.001), respectively. No significant improvements of SRS-22 function domain (t = -2.825, P = 0.088) and mental health domain (t = -3.174, P = 0.061) were observed. The mean Cobb angle decreased from 28.9 ± 5.5° to 26.3 ± 5.2° at the final follow-up, despite no statistical significance was observed (t = 1.853, P = 0.102). The mean C2-C7 SVA value decreased from 21.7 ± 8.4 mm to 17.0 ± 8.0 mm (t = -1.224 P = 0.049) and mean T1 tilt decreased from 4.9 ± 4.2 ° to 3.5 ± 3.1° (t = 2.913, P = 0.011). No significant improvement of radiographic parameters and HRQOL were observed in observation group.@*CONCLUSIONS@#For AIS patients with a Risser 3-5 and a Cobb angle 20°-40°, Schroth exercises improved HRQOL and halted curve progression during the follow-up period. Both cervical spine alignment and shoulder balance were also significantly improved after Schroth exercises. We recommend Schroth exercises for patients with AIS.


Subject(s)
Adolescent , Adult , Cervical Vertebrae , Humans , Kyphosis , Lordosis , Quality of Life , Reproducibility of Results , Retrospective Studies , Scoliosis/therapy , Treatment Outcome
6.
Article in English | WPRIM | ID: wpr-880856

ABSTRACT

The goal of this study was to identify MSX1 gene variants in multiple Chinese families with nonsyndromic oligodontia and analyse the functional influence of these variants. Whole-exome sequencing (WES) and Sanger sequencing were performed to identify the causal gene variants in five families with nonsyndromic oligodontia, and a series of bioinformatics databases were used for variant confirmation and functional prediction. Phenotypic characterization of the members of these families was described, and an in vitro analysis was performed for functional evaluation. Five novel MSX1 heterozygous variants were identified: three missense variants [c.662A>C (p.Q221P), c.670C>T (p.R224C), and c.809C>T (p.S270L)], one nonsense variant [c.364G>T (p.G122*)], and one frameshift variant [c.277delG (p.A93Rfs*67)]. Preliminary in vitro studies demonstrated that the subcellular localization of MSX1 was abnormal with the p.Q221P, p.R224C, p.G122*, and p.A93Rfs*67 variants compared to the wild type. Three variants (p.Q221P, p.G122*, and p.A93Rfs*67) were classified as pathogenic or likely pathogenic, while p.S270L and p.R224C were of uncertain significance in the current data. Moreover, we summarized and analysed the MSX1-related tooth agenesis positions and found that the type and variant locus were not related to the severity of tooth loss. Our results expand the variant spectrum of nonsyndromic oligodontia and provide valuable information for genetic counselling.


Subject(s)
Anodontia/genetics , Humans , MSX1 Transcription Factor/genetics , Pedigree , Whole Exome Sequencing
7.
Article in Chinese | WPRIM | ID: wpr-865926

ABSTRACT

Objective:To study the utilization of massive open online courses (MOOCs) learning and the satisfaction with the learning effect of MOOCs.Methods:A questionnaire survey was formulated for residents' cognition of MOOCs in standardized residency training, and 133 valid questionnaires were collected. Descriptive statistical analysis, chi-square test and logistic regression were used to analyze the influencing factors of residents' learning satisfaction with MOOCs.Results:The influencing factors of standardized residency training trainees' satisfaction with MOOCs were the flexibility of learning time, the setting of discussion area, the interaction with teachers and the learning enthusiasm of the trainees.Conclusion:Standardized residency training trainees are satisfied with the learning effect of MOOCs. In the future, the construction of MOOCs will focus on formulating regular learning plan and supervision mechanism, increasing communication and feedback between teachers and trainees, improving the learning enthusiasm of residential trainees and enriching the content of MOOCs.

8.
Article in Chinese | WPRIM | ID: wpr-863239

ABSTRACT

Artificial intelligence not only has massive data storage capacity and powerful data analysis capability, but also can simulate human consciousness and thinking patterns using language recognition, image recognition, natural language processing and other technologies. At present, artificial intelligence has been integrated into every field of society, especially played an important role in the healthcare field of China. In this paper, the relevant literatures published in recent years about artificial intelligence in the healthcare field of China were reviewed and sorted, and the hotspots of artificial intelligence research were analyzed. The main application scenarios of artificial intelligence in this field were investigated and analyzed by classification and statistics. Finally, some challenges faced by artificial intelligence in promoting the healthcare of China were discussed and corresponding suggestions were proposed.

9.
Article in English | WPRIM | ID: wpr-878348

ABSTRACT

Objective@#We aimed to compare the clinical and radiological outcomes of midline lumbar fusion (MIDLF) versus minimally invasive transforaminal lumbar interbody fusion (MI-TLIF) in patients with degenerative spondylolisthesis and/or stenosis in L4-L5 two years after surgery.@*Methods@#Consecutively treated patients with lumbar pathology who underwent MIDLF ( @*Results@#The mean operative time and hematocrit (HCT, Day 1) were significantly shorter and lower in MIDLF cases (174 min @*Conclusion@#MIDLF is comparable to MI-TLIF at L4-5 in clinical outcomes and fusion rates, and the results verified the meaningful advantage of using MIDLF for the elderly with osteoporosis.


Subject(s)
Aged , Female , Humans , Lumbar Vertebrae/surgery , Male , Middle Aged , Retrospective Studies , Spinal Fusion/methods , Treatment Outcome
10.
Article in Chinese | WPRIM | ID: wpr-873291

ABSTRACT

Objective::To explore the effect of Dioscoreae Nipponicae Rhizoma extract (DNRe) on rats with acute gouty arthritis (AGA) based on urine metabolomics and to search for the related potential biomarkers and metabolic pathways. Method::Rat model of AGA induced by monosodium urate (MSU) was selected, 40 rats were randomly divided into the blank group (k), the DNRe group (g), the model group (m), and the DNRe treatment group (gm), with 10 rats in each group. The drug-administered group was administered with DNRe at a dose of 0.48 g·kg-1 once a day for 5 days. The urine was gathered after the last administration, and analyzed with UPLC-Q-TOF/MS coupled with pattern recognition techniques, electrospray ionization (ESI) under positive and negative ion scanning mode was adopted, data collection range was m/z 100-1 500 with full scanning mode. Result::A total of 12 common potential biomarkers were identified as sarcosine, dimethylglycine, deoxycytidine, uric acid, 5-hydroxytryptamine (5-HT), L-cystathionine, 4-pyridoxic acid, deoxyuridine, melatonin, 5-methoxytryptamine, fumaric acid and cytidine. Compared with the blank group, the 12 potential biomarkers in the DNRe group were significantly down-regulated. Compare with the model group, 10 metabolites were up-regulated and 2 metabolites were down-regulated in the 12 potential biomarkers of the DNRe treatment group, the abnormal expression of 10 markers including sarcosine, uric acid, L-cystathionine, 4-pyridoxic acid, deoxyuridine, 5-methoxytryptamine, cytidine, dimethylglycine, melatonin, fumaric acid could be modulated by DNRe. The strongest metabolic pathways associated with AGA were cysteine and methionine metabolism, and tryptophan metabolism. Conclusion::The effect of DNRe on AGA may be related to the promotion of conversion level from cystathionine to cysteine in the cysteine and methionine metabolism, and the up-regulating melatonin level in tryptophan metabolism.

11.
Article in Chinese | WPRIM | ID: wpr-873128

ABSTRACT

Objective::To observe the effect of Ganoderma polysaccharides (GP) on endogenous substance metabolism in radiation-injured mice by metabolomics, so as to find potential biomarkers and analyze their metabolic pathways, and to explore its mechanism of action. Method::Thirty mice were randomly divided into normal group (normal saline), model group (normal saline) and GP group (dose of 96 mg·kg-1) for 14 days of continuous intragastric administration, 10 mice in each group, 2 h after the intragastric administration on the 7th day, mice in the model group and GP group were subjected to whole body irradiation by X-rays, except the normal group. UPLC-Q-TOF-MS was used to detect endogenous small molecule metabolites in thymus tissue of mice. Principal component analysis (PCA)and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to compare the changes of endogenous small molecule metabolites in thees three groups, these differential metabolites among the three groups were analyzed by Kyoto encyclopedia of genes and genomes (KEGG) metabolic pathway method. Result::A total of 34 potential biomarkers were identified, compared with the model group, it was found that the GP group had a significant reversal trend on L-glutamic acid, taurine, phosphatidylcholine (PC) and lysophosphatidylcholine (LysoPC), etc. They were involved in taurine and hypotaurine metabolism, D-glutamine and D-glutamate metabolism, glycerophospholipid metabolism. Conclusion::GP can play a role in radiation protection by improving the expression of related potential biomarkers and related metabolic pathways in thymus of radiation-injured mice.

12.
Article in Chinese | WPRIM | ID: wpr-872746

ABSTRACT

Scutellarin is a flavonoid extracted from breviscapus, a traditional Chinese medicine. Pharmacological studies have shown that scutellarin has anti-inflammatory, antioxidant, anti-fibrosis, anti-tumor, improving cardiac and cerebral ischemia. In recent years, with the deepening of research on scutellarin, it was found that it could inhibit the tumor through multi-target and multi-pathway, and the anti-human colorectal cancer was related to the regulation of p53 pathway, Hedgelog pathway and erythropoietin generates liver cancer interactivator B2(EphrinB2).The anti-esophageal squamous cell carcinoma is related to protein kinaseB1 /protein kinaseB2( Akt1/Akt2).Anti-renal carcinoma and melanoma are associated with phosphatase and tension protein homologues(PTEN) and phosphatidylinositol 3-kinase(PI3K)/Akt/mammalian target of rapamycin(mTOR) pathway. Anti-lung cancer is related to Akt/mTOR/4E binding protein1(4EBP1) and signal transduction and transcriptional activator(STAT3 )signaling pathway. Anti-cervical cancer is related to pyruvate kinase 2(PKM2).Anti-breast cancer is associated with Hippo/YAP pathway. At the same time, scutellarin was found to prevent diabetic microangiopathy, atherosclerosis and non-alcoholic fatty liver disease by regulating glucose and lipid metabolism, but the mechanism of action was not well studied. A review of the literature found that scutellarin anti-tumor, atherosclerosis, diabetic microangiopathy, non-alcoholic fatty liver disease, osteoarthritis, osteoporosis mechanism of action lack of detailed summary. In this paper, the research progress of pharmacological action and mechanism of scutellarin in recent 5 years is reviewed, and Suggestions on its current research status and future direction are put forward, in order to speed up the discovery of pharmacological mechanism of scutellarin and provide scientific basis for its further development and utilization.

13.
Chinese Journal of Dermatology ; (12): 992-997, 2020.
Article in Chinese | WPRIM | ID: wpr-870391

ABSTRACT

Objective:To assess the reliability and validity of the Chinese version of chronic urticaria quality of life questionnaire (CU-Q2oL) .Methods:The original English version of CU-Q2oL was translated into Chinese after forward-backward translation and cultural adaption, and items of the Chinese version of CU-Q2oL were determined. From January to December 2019, 195 chronic spontaneous urticaria patients with or without chronic inducible urticaria were enrolled from Department of Dermatology and Venereology, Peking University First Hospital, and followed up every 2 weeks. A total of 2 follow-ups were carried out, and a questionnaire survey was conducted by using the Chinese version of CU-Q2oL, dermatology life quality index (DLQI) and urticaria activity score over 7 days (UAS7) during each follow-up. After each follow-up, the treatment protocol for each patient was adjusted by clinicians based on the patient′s condition. The number of questionnaire factors was extracted by exploratory factor analysis, convergent validity was estimated by analyzing correlations of CU-Q2oL with DLQI and UAS, and the internal consistency reliability of CU-Q2oL was evaluated by calculating Cronbach′s α coefficient; the sensitivity of CU-Q2oL was assessed by analyzing correlations of changes in UAS7 scores with changes in CU-Q2oL scores.Results:Four factors were extracted from the Chinese version of CU-Q2oL, namely "symptoms", "daily functional activities", "sleep problems" and "restrictions" factors. There were 23 items in total, and the cumulative variance contribution rate was 74.011%. The Cronbach′s α coefficient of all items in CU-Q2oL was 0.961, and the Cronbach′s α coefficient of the 4 factors ranged from 0.804 to 0.933, suggesting excellent internal consistency. The total score of CU-Q2oL was moderately correlated with DLQI and UAS7 scores, with correlation coefficients of 0.437 and 0.560, respectively (both P < 0.01) . A total of 71 patients completed the second follow-up. The change in the total score of CU-Q2oL was moderately correlated with that in the UAS7 score, with a correlation coefficient of 0.392 ( P < 0.01) . Conclusion:The Chinese version of CU-Q2oL has excellent internal consistency, good reliability and validity, and can be used to evaluate the quality of life of patients with chronic spontaneous urticaria in China.

14.
Chinese Journal of Dermatology ; (12): 698-703, 2020.
Article in Chinese | WPRIM | ID: wpr-870342

ABSTRACT

Objective:To evaluate the reliability, validity and sensitivity of the Chinese version of pruritus-specific quality of life instrument (ItchyQoL) .Methods:Based on the English version of ItchyQoL, items of the Chinese version of ItchyQoL were determined after forward-backward translation and cultural adaption. Totally, 218 patients with pruritus caused by skin diseases were enrolled from Department of Dermatology, Peking University First Hospital from January to December 2019, and a questionnaire survey was conducted. At the time of enrollment and 2 weeks after enrollment, the Chinese version of ItchyQoL, dermatology life quality index (DLQI) and numerical rating scale (NRS) were used to evaluate the effect of pruritus on the quality of life of patients. Confirmatory factor was used to assess the structural validity of the Chinese version of ItchyQoL, and Cronbach′s α coefficient was used to estimate its internal consistency reliability. Spearman correlation coefficient was used to analyze correlations (convergent validity) of the Chinese version of ItchyQoL score with DLQI and NRS scores, and correlations (sensitivity) of changes in the Chinese version of ItchyQoL score with changes in DLQI and NRS scores.Results:The Chinese version of ItchyQoL contained a total of 22 items, including 3 dimensions, i.e., "symptoms", "functions" and "emotions", with the Cronbach′s α coefficients being 0.946, 0.883 and 0.953 respectively, suggesting excellent internal consistency. At 2 weeks after enrollment, the NRS score was strongly correlated with the total ItchyQoL score ( rs = 0.700, P < 0.01) , and moderately correlated with the subscores of "emotions", "functions" and "symptoms" ( rs = 0.452, 0.673, 0.692 respectively, all P < 0.01) ; the DLQI score was also strongly correlated with the total ItchyQoL score ( rs = 0.887, P < 0.01) and the subscores of "functions" and "symptoms" ( rs = 0.886, 0.750 respectively, both P < 0.01) , and moderately correlated with the "emotions" subscore ( rs = 0.674, P < 0.01) . Compared with the scores at the time of enrollment, the change in the total ItchyQoL score after 2 weeks was moderately correlated with the change in the NRS score ( rs = 0.642, P < 0.01) , and strongly correlated with the change in the DLQI score ( rs = 0.757, P < 0.01) ; the changes in "symptoms" and "functions" subscores were moderately correlated with the change in the NRS score ( rs = 0.648, 0.549 respectively, both P < 0.01) , while there was a weak correlation between the changes in "emotions" subscore and NRS score ( rs = 0.225, P < 0.01) ; the changes in "symptoms" and "functions" subscores were strongly correlated with the change in the DLQI score ( rs = 0.755, 0.703 respectively, both P < 0.01) , while the change in "emotions" subscore was moderately correlated with the change in the DLQI score ( rs = 0.401, P < 0.01) . Moreover, the total score of the Chinese version of ItchyQoL and subscores of "symptoms", "functions" and "emotions" all significantly differed among different NRS and DLQI score groups at 2 weeks after enrollment (all P < 0.05) . Conclusion:The Chinese version of ItchyQoL has good reliability and validity, and can be used to evaluate the quality of life of patients with pruritus caused by skin diseases in China.

15.
Chinese Journal of Dermatology ; (12): 533-538, 2020.
Article in Chinese | WPRIM | ID: wpr-870324

ABSTRACT

Objective:To translate the Urticaria Control Test (UCT) into Chinese, and to assess reliability, validity, sensitivity and screening accuracy of the new-version scale.Methods:After forward-backward translation and cultural adaption, items of the Chinese version of UCT were determined. The scale was used in 51 patients with chronic spontaneous urticaria (CSU) , 41 with chronic inducible urticaria (CIndU) , and 11 with CSU complicated by CIndU. Within 8 weeks after enrollment, 81 patients were treated with antihistamines, 8 with omalizumab, and 14 with antihistamines combined with omalizumab. At the time of enrollment and 4 and 8 weeks after enrollment, dermatology life quality index (DLQI) and urticaria activity score (UAS) were used to assess the quality of life impairment and disease activity. The internal consistency reliability of the questionnaire was estimated using Cronbach′s α coefficient. By comparing with DLQI and UAS28 scores, the Chinese version of UCT was tested for convergent validity, known-group validity, sensitivity and screening accuracy.Results:The Chinese version of UCT contained 4 items, and could be used to retrospectively evaluate clinical symptoms and signs of the disease, impact on quality of life, treatment effects and overall disease control in the past 4 weeks. The Cronbach′s α coefficient of each item in the UCT scale was 0.886 - 0.945 in the CSU group, and 0.834 - 0.958 in the CIndU group. At the time of enrollment, the UCT score was significantly negatively correlated with the DLQI score in both the CSU group and CIndU group ( rs = -0.672, -0.578, respectively, both P < 0.01) . At 4 and 8 weeks, the UCT score was significantly negatively correlated with UAS28 and DLQI scores in the CSU group (4 weeks: rs = -0.654, -0.829, respectively, both P < 0.01; 8 weeks: rs = -0.717, -0.765, respectively,both P < 0.01) , and it was also significantly negatively correlated with the DLQI score in the CIndU group ( rs = -0.834, -0.778, respectively, both P < 0.01) . In the CSU group, the change in the UCT score between weeks 4 and 8 was significantly negatively correlated with the change in UAS score ( rs = -0.569, P < 0.01) ; compared with the baseline, the change in the UCT scores was also significantly negatively correlated with the change in the DLQI scores at weeks 4 and 8 ( rs = -0.693, -0.447, respectively, both P < 0.01) . In the CIndU group, compared with the baseline, the change in the UCT scores at weeks 4 and 8 also showed a significant correlation with the change in DLQI scores ( rs = -0.615, -0.408, respectively, both P < 0.01) . The UCT score significantly differed among different UAS and DLQI score groups (all P < 0.05) . Conclusions:The Chinese version of the UCT is a valid and reliable tool for the clinical management of patients with CSU/CIndU. It can be used to evaluate disease control, and reflect disease activity and disease-related quality of life to a certain extent.

16.
Article in Chinese | WPRIM | ID: wpr-869912

ABSTRACT

Objective:To evaluate the effect of sufentanil on activation of Schwann cells after peripheral nerve injury in mice.Methods:Eighty healthy pathogen-free male Balb/c mice, aged 6-8 weeks, weighing 18-22 g, were divided into 4 groups ( n=20 each) using a random number table method: peripheral nerve injury group (group PNI), high dose sufentanil group (group H), medium dose sufentanil group (group M) and low dose sufentanil group (group L). The model of unilateral sciatic nerve transaction was established in ketamine-anesthetized mice.Immediately after establishment of the model, sufentanil 10, 5 and 2.5 μg/kg was injected intraperitoneally once a day for 3 consecutive days in H, M and L groups, respectively, while the equal volume of normal saline was given instead in group PNI.Sciatic function index (SFI) was calculated at 4, 8 and 12 weeks after establishment of the model.At 2, 4, 8 and 12 weeks, 5 mice in each group were sacrificed, and segments of the injuried ipsilateral sciatic nerve were removed for examination of the ultrastructure of the sciatic nerve (with a transmission electron microscope) and for detection of the expression of glial fibrillary acidic protein (GFAP) of sciatic nerve (by immunohistochemistry). Results:Compared with group PNI, SFI was significantly increased, and the expression of GFAP was up-regluated at each time point after establishment of the model in H and M groups ( P<0.05) and no significant change was found in SFI and GFAP expression after establishment of the model in group L ( P>0.05). Compared with group L, SFI was significantly increased, and GFAP expression was up-regluated in H and M groups ( P<0.05). There was no significant difference in SFI and GFAP expression between group H and group M ( P>0.05). The thickness of myelin lamellae was dense, and the proliferation of Schwann cells was not marked in H and M groups.The thickness of myelin lamellae was thin, and the proliferation of Schwann cells was marked in L and MO groups. Conclusion:The mechanism by which sufentanil improves repair after peripheral nerve injury may be related to promoting activation of Schwann cells in mice.

17.
Chinese Journal of Orthopaedics ; (12): 1540-1548, 2020.
Article in Chinese | WPRIM | ID: wpr-869107

ABSTRACT

Objective:to review the clinical features, diagnosis and treatment of spinal Rosai-Dorfman disease(RDD).Methods:we conducted a systemic review and collected the cases reported from 2010. The key words were Rosai-Dorfman disease, spine/central nervous system. We screened both English and Chinese database. There were 43 reports finally included in the study, containing 52 cases. We distracted the information of interest and, subsequently, analyzed the harvested data using specific statistical software packages. The study focused on the summary and description of the clinical features, diagnosis and treatment and prognosis of spinal RDD.Results:The included articles reported 52 cases. The average age was 32.1±17.1 years (ranging from 6 to 76 years old). The ratio of male to female was 1.9/1. The median follow-up period was 19.9 months. The initial symptoms of 41 patients (78.8%) were spinal lesion-related. The cases with painless lymph nodes enlargement, other organ lesions and abnormal lab tests were 11.5%, 36.5% and 23.1%, respectively. The frequent infringed segments were cervical (43.1%) and thoracic (39.2%) spine. 53.2% lesions were dura-based, while 17.0% and 10.6% for bone and cord, respectively. Surgery was the mainstream of the treatment armamentarium, composing 83.7% cases, among which 46.3% underwent total resection. Cases only treated with radiotherapy, chemotherapy and steroids were 10.2%. Very Few cases remitted spontaneously (2.0%). The risk of recurrence and occurrence at other vertebral levels was 22.0%.Conclusion:It is rare for spinal involvement of RDD. This entity has no pathognomonic clinical and imaging features. RDD has a tendency of multi-organ involvement and recurrence. Surgery remains the mainstay of the treatment, but the efficacy of other adjuvant therapies is not sure. A wait and watch strategy is employed for asymptomatic patients.

18.
Article in English | WPRIM | ID: wpr-827079

ABSTRACT

OBJECTIVES@#To develop a new Chinese medicine (CM)-based drug and to evaluate its safety and effect for suppressing acute respiratory distress syndrome (ARDS) in COVID-19 patients.@*METHODS@#A putative ARDS-suppressing drug Keguan-1 was first developed and then evaluated by a randomized, controlled two-arm trial. The two arms of the trial consist of a control therapy (alpha interferon inhalation, 50 µg twice daily; and lopinavir/ritonavir, 400 and 100 mg twice daily, respectively) and a testing therapy (control therapy plus Keguan-1 19.4 g twice daily) by random number table at 1:1 ratio with 24 cases each group. After 2-week treatment, adverse events, time to fever resolution, ARDS development, and lung injury on newly diagnosed COVID-19 patients were assessed.@*RESULTS@#An analysis of the data from the first 30 participants showed that the control arm and the testing arm did not exhibit any significant differences in terms of adverse events. Based on this result, the study was expanded to include a total of 48 participants (24 cases each arm). The results show that compared with the control arm, the testing arm exhibited a significant improvement in time to fever resolution (P=0.035), and a significant reduction in the development of ARDS (P=0.048).@*CONCLUSIONS@#Keguan-1-based integrative therapy was safe and superior to the standard therapy in suppressing the development of ARDS in COVID-19 patients. (Trial registration No. NCT04251871 at www.clinicaltrials.gov ).


Subject(s)
Administration, Inhalation , Adult , China , Coronavirus Infections , Diagnosis , Drug Therapy , Mortality , Dose-Response Relationship, Drug , Drug Administration Schedule , Drugs, Chinese Herbal , Female , Follow-Up Studies , Humans , Integrative Medicine , Interferon-alpha , Lopinavir , Male , Middle Aged , Pandemics , Pneumonia, Viral , Diagnosis , Drug Therapy , Mortality , Risk Assessment , Severe Acute Respiratory Syndrome , Diagnosis , Drug Therapy , Mortality , Severity of Illness Index , Survival Rate
19.
Article in Chinese | WPRIM | ID: wpr-827972

ABSTRACT

Fourteen compounds were isolated from the ethanol extract of Dalbergiae Odoriferae Lignum by various chromatographic techniques, including column chromatographies on silica gel, Sephadex LH-20 and semi-preparative HPLC. Their structures were determined by spectroscopic techniques as S-3'-hydroxy-7,2',4'-trimethoxyisoxane(1), 2-(2',4'-dimethoxyphenyl)-6-hydroxybenzofuran(2), 2-(2'-hydroxy-4'-methoxyphenyl)-6-methoxybenzofuran(3), 7,2',4'-trimethoxydihydroisoflavone(4), sativanone(5), 3,9-dimethoxy-6H-benzofuro[3,2-c]chromen-6-one(6),(6 aS,11 aS)-homopterocarpin(7),(6 aS,11 aS)-8-hydroxy-3,9-dimethoxypterocarpan(8),(6 aS,11 aS)-3,8,9-trimethoxypterocarpan(9), isodalbergin(10), isoliquiritigenin(11), butein(12), butin(13) and 3,7,4'-trihydroxyflavone(14). Among them, compound 1 was a new compound, while 2 and 3 were new natural products, 6, 8, 9 and 14 were isolated for the first time from Dalbergiae Odoriferae Lignum. Compounds 1-14 were tested for their cytotoxic activity against human hepatoma cell line BEL-7402, human gastric cancer cell line SCG-7901, human lung cancer cell line A549, human chronic myeloid leukemia cell line K562 and HeLa human cervical cancer cellline by MTT method. Compound 1 exhibited significant cytotoxicity with IC_(50) values ranging from 2.85 to 11.62 μg·mL~(-1). In addition, 2, 11 and 12 showed weak cytotoxic activities.


Subject(s)
Antineoplastic Agents , Chromatography, High Pressure Liquid , HeLa Cells , Humans
20.
Chinese Medical Journal ; (24): 909-918, 2020.
Article in English | WPRIM | ID: wpr-827684

ABSTRACT

BACKGROUNDS@#Cervical posterior decompression surgery is used to relieve ventral compression indirectly by incorporating a backward shift of the spinal cord, and this indirect decompression is bound to be limited. This study aimed to determine the decompression limit of posterior surgery and the effect of the decompression range.@*METHODS@#We retrospectively reviewed the data of 129 patients who underwent cervical open-door laminoplasty through 2008 to 2012 and were grouped as follows: C4-C7 (n = 11), C3-C6 (n = 61), C3-C7 (n = 32), and C2-C7 (n = 25). According to the relative location of spinal levels within a decompression range, the type of decompression at a given level was categorized as external decompression (ED; achieved at the levels located immediately external to the decompression range margin), internal decompression (ID; achieved at the levels located immediately internal to the decompression range margin), and central decompression (CD; achieved at the levels located in the center, far from the decompression range margin). The vertebral-cord distance (VCD) was used to evaluate the decompression limit. The C2-C7 angle and VCD on post-operative magnetic resonance images were analyzed and compared between groups. The relationship between VCD and decompression type was analyzed. Moreover, the relationship between the magnitude of the ventral compressive factor and the probability of post-operative residual compression at each level for different decompression ranges was studied.@*RESULTS@#There was no significant kyphosis in cervical curvature (> -5°), and there was no significant difference among the groups (F = 2.091, P = 0.105). The VCD of a specific level depended on the decompression type of the level and followed this pattern: ED < ID < CD (P < 0.05). The decompression type of a level was sometimes affected by the decompression range. For a given magnitude of the ventral compressive factor, the probability of residual compression was lower for the group with the larger VCD at this level.@*CONCLUSIONS@#Our study suggests that the decompression range affected the decompression limit by changing the decompression type of a particular level. For a given cervical spinal level, the decompression limit significantly varied with decompression type as follows: ED < ID < CD. CD provided maximal decompression limit for a given level. A reasonable range of decompression could be determined based on the relationship between the magnitude of the ventral compressive factor and the decompression limits achieved by different decompression ranges.

SELECTION OF CITATIONS
SEARCH DETAIL