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1.
Article in Chinese | WPRIM | ID: wpr-920542

ABSTRACT

Objective To explore the effects of different dietary induction models of inulin, resistant starch RS3 and their complexes on the body weight and intestinal flora in mice. Methods A total of 64 C57BL/6 mice were randomly divided into low-fat control group, low-fat inulin group, low-fat resistant starch RS3 group, low-fat composite group and high-fat control group, high-fat inulin group, high-fat resistant starch RS3 group and high-fat composite group for dietary intervention. The mice were weighed and fresh feces were collected weekly. Diet intervention was continued until the weight of the high-fat control group was more than 14% higher than that of the low-fat control group. The mice were then sacrificed after overnight fasting. Liver and epididymal fat were weighed, and the colon contents were collected for 16S amplicon sequencing analysis. Results In low-fat diet fed mice, the combined induction of inulin and resistant starch RS3 caused a significant decrease in body weight gain. In high-fat diet fed mice, inulin alone and the combined induction both caused a significant reduction in weight gain, and there was no significant difference between the two methods. In the high-fat diet groups, inulin, resistant starch RS3, and the compound could be distinguished by Bacteroides, Bifidobacterium and Alloprevotella respectively. In the low-fat diet groups, inulin, resistant starch RS3, and the composite groups could be distinguished by Coriobacteriaceae_UCG_002, Bacteroides and Helicobacter, respectively. Conclusion Inulin and resistant starch RS3 diet induction can significantly reduce the weight gain of C57BL/6 mice, change the structure of intestinal flora, and show the difference between high-fat and low-fat diets. Inulin and resistant starch RS3 may reduce body weight and promote fat metabolism by changing the structure of intestinal flora.

2.
Chinese Journal of Biotechnology ; (12): 2976-2983, 2021.
Article in Chinese | WPRIM | ID: wpr-887859

ABSTRACT

Life sciences are the disciplines most closely related with human beings. As experimental disciplines, life sciences develop rapidly and highly intersect in many scientific fields. Under the "double first-class" initiative, the comprehensive development-oriented talent training system has put forward an urgent need for life sciences literacy and comprehensive ability training of college students. Taking the reform of liberal education curriculum system as an opportunity, we developed a series of eight life sciences practical liberal courses for students with non-biology majors. The courses cover all sub-disciplines or directions of life sciences, and aim to foster interdisciplinary talents with life sciences knowledge and literacy, as well as practical and innovative abilities. These courses could serve as references for experimental teaching centers in colleges and universities to set up practical liberal and experimental courses.


Subject(s)
Biological Science Disciplines , Curriculum , Humans , Students , Universities
3.
Article in Chinese | WPRIM | ID: wpr-912639

ABSTRACT

Objective:To use three-dimensional (3D) scanning to measure the preoperative and postoperative nasal parameters of the patients received rhinoplasty with ear cartilage and silicone prosthesis, and to evaluate the clinical effect of the surgery.Methods:Sixteen female patients with an average age of 28.3 years, ranged from 21 to 35 years, received rhinoplasty with ear cartilage and silicone prosthesis in Wuhan Tongji Hospital from June 2018 to February 2019. Preoperative and postoperative 3D scanning was performed to measure nasal parameters, including linear length, angle, and proportional index.Results:All patients were satisfied with the postoperative outcomes. The postoperative nasal length, nasal height, and nasal depth increased significantly, and the postoperative nasal width and nasal tip width decreased. The postoperative nasolabial angle and nasofrontal angle were statistically improved, while the preoperative and postoperative data of columellar facial angle, nasal tip angle, and nasal column-lobular angle were not significantly different. The ratios of nasal depth and nasal width, nasal index and nasal tip protrusion were improved after surgery, while the postoperative ratios of nasal columella and nasal lobules length were not statistically improved.Conclusions:The 3D scanning allows for comprehensive and accurate measurement of the nasal parameters. The rhinoplasty with ear cartilage and silicone prosthesis is more effective in improving the overall contour of the nose, but less effective in improving the aesthetics of the nasal tip.

4.
Chinese Journal of Microsurgery ; (6): 517-520, 2021.
Article in Chinese | WPRIM | ID: wpr-912272

ABSTRACT

Objective:To explore a comparative study of arterial end-to-end and end-to-side anastomosis in superficial branch of the superficial circumflex iliac artery(SCIA) perforator flap transfer.Methods:Between November, 2019 and December, 2020, 21 patients with the soft tissue defects in the limbs were repaired with superficial branch of the SCIA perforator flaps. The size of flaps ranged from 3.5 cm×7.0 cm to 9.0 cm×18.0 cm. According to the upper or anterior wall of the main artery in the recipient area having branches that matched the flap artery, 2 groups were established. End-to-end group: 10 cases were anastomosed end-to-end between the flap artery and branch of the main artery in the recipient area; End-to-side group: 11 cases were anastomosed end-to-side between the flap artery and side mouth of the main artery in the recipient area. The vein of flap was anastomosed end-to-end with the accompanying vein to the main artery in the recipient area. All of the donor sites were sutured directly. All patients were followed-up for 6-12 months and the survival of the perforator flap, the appearance and function of the perforator flap and the donor site were observed. All data of the 2 groups were conducted statistical analyzed. P<0.05 was statistically significant. Results:All 10 flaps in end-to-end group survived successfully. In end-to-side group, 2 cases had venous crisis in 11 cases of flaps,the exploration revealed venous thrombosis, and the arterial end-to-side anastomosis had smooth blood flow had embolism. One flap survived after re-anastomosis of the vein, and 1 flap was changed to a pedicled abdominal flap during the re-venous crisis. The postoperative follow-up was 6 months to 1 year. The appearance and function of the flap and donor site were satisfactory, without difference between the 2 groups. The SCIA superficial branch artery caliber, recipient artery branch or lateral caliber was not statistically different between the 2 groups( P>0.05). The time of anastomosis for end-to-end group was[(16.70±1.34) min]. It was lower than that of anastomosed end-to-side group[(23.73±1.68) min]. The difference was statistically significant( P<0.01). Conclusion:In superficial branch of the SCIA perforator flap transfer, if the upper or anterior wall of the main artery in the recipient area has a branch that matches the flap artery, the flap artery should first be anastomosed with its end. Because it dose not required to make a side port, and makes the operation more convenient with a short anastomosis time; Otherwise, perform end-to-side anastomosis with the main artery of the recipient site.

5.
Article in Chinese | WPRIM | ID: wpr-911461

ABSTRACT

Objective:To compare the effectiveness of first-line mechanical thrombectomy (MT) with other types of endovascular treatment (EVT) in patients with acute vertebrobasilar artery occlusion (VBAO).Methods:From May 2012 to December 2019, acute VBAO patients diagnosed by angiographic examinations were consecutively enrolled from Nanjing Stroke Registry. Patients were divided into first-line MT group who were treated with stent retriever and direct aspiration, and other types of EVT group who underwent intraarterial thrombolysis, tirofiban infusion, balloon dilation, and stent placement etc. Clinical characteristics at baseline with statistical trends ( P<0.1) between different groups were analyzed to calculate propensity scores, which were adjusted in binary Logistic regression for the relationship between first-line treatment and clinical outcome. Results:A total of 107 patients were enrolled in this study including 82 males and 25 females with average age 62 years old (35-82). The overall recanalization rate was 82.2% (88/107). During follow-up, 33.6% (36/107) and 37.1% (39/105) patients achieved good outcome (defined as modified Rankin Scale score 0-3) at 90 days and 1 year, respectively. The MT group received EVT earlier than the other types of EVT group (median year: 2017 vs. 2015, P=0.017), as well as higher proportion of atrial fibrillation history (21.6% vs. 0, P=0.002), higher baseline National Institution of Health Stroke Scale scores (median: 26 vs. 23, P=0.049), lower Glasgow Coma Scale scores (median: 6 vs. 7, P=0.027), and longer estimated occlusion to groin-puncture time (median: 367 min vs. 283 min, P=0.023). There were significant differences in the stroke etiologies between two groups ( P=0.002). The MT group had a lower ratio of rescue device treatment (28.4% vs. 54.5%, P=0.009). After propensity scores were adjusted, the recanalization rate of MT group was significantly higher than that in the other group [odds ratio (95% confidence interval [ CI]):5.201, 95 %CI 1.562-17.317, P=0.007]. No difference was noted regarding other clinical outcomes. Subgroup analysis indicated that recanalization rates in patients without atherosclerosis were different between intervention types [odds ratio (95 %CI): 7.859, 95 %CI 1.469-42.042, P=0.016], while the recanalization rates was comparable in population with atherosclerosis [odds ratio (95 %CI): 3.739, 95 %CI 0.613-22.812, P=0.153]. Conclusion:In acute VBAO patients, first-line MT is associated with higher recanalization rate compared with other types of EVT, especially in non-atherosclerosis patients.

6.
Article in Chinese | WPRIM | ID: wpr-911180

ABSTRACT

Objective:To evaluate the effect of erector spinae plane (ESP) block combined with general anesthesia on early postoperative cognitive function in elderly patients undergoing thoracoscopic radical resection of lung cancer.Methods:Sixty patients of both sexes, aged ≥65 yr, with body mass index< 30 kg/m 2, of American Society of Anesthesiologists physical status Ⅱ or Ⅲ, scheduled for single-port thoracoscopic radical resection of lung cancer, were divided into 2 groups ( n=30 each) using a random number table method: ESP block plus general anesthesia group (group ESP+ GA) and general anesthesia group (group GA). Ultrasound-guided ESP was performed at T 4 before induction of general anesthesia in group ESP+ GA.The success of the nerve block was determined by pin-prick test at 20 min after nerve block, and then anesthesia was induced.Anesthesia was maintained with propofol plus remifentanil in the two groups.Patient-controlled intravenous analgesia was performed.Venous blood samples were collected at 1 day before operation and 1, 2 and 3 days after operation for determination of plasma S100β protein and neuron-specific enolase (NSE) concentrations by enzyme-linked immunosorbent assay.At 1 day before operation and 3, 5, and 7 days after operation, the early cognitive function was assessed using the Mini-Mental State Examination (MMSE), and the occurrence of postoperative cognitive dysfunction within 7 days after operation was recorded.The intraoperative consumption of propofol, remifentanil and sufentanil was recorded.The occurrence of complications related to ESP block was recorded. Results:Compared with group GA, the consumption of propofol, remifentanil, and sufentanil was significantly reduced, the serum S100β protein concentration and Mini-Mental State Examination score were decreased at 1 and 3 days after operation, serum NSE concentrations were decreased at 1, 3 and 5 days after operation, and the incidence of postoperative cognitive dysfunction was decreased in group ESP+ GA ( P<0.05). ESP block was successful, and no vascular and nerve damage was found in group ESP+ GA. Conclusion:ESP block combined with general anesthesia can improve the early cognitive function after thoracoscopic radical resection of lung cancer in elderly patients.

7.
Article in Chinese | WPRIM | ID: wpr-910580

ABSTRACT

Objective:To investigate the effect of logistic regression model based on virtual touch tissues quantification (VTQ) and fibrosis index based on four factors (FIB-4) in assessing impaired liver reserve function (LFR) in hepatic surgery patients before surgical resection.Methods:From January 2016 to October 2018, 173 patients including 135 males and 38 females with the mean age of 58.6 years old, scheduled for potential hepatectomy in Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical School, were enrolled in our retrospective study. According to indocyanine green retention test at 15 minutes (ICG R15), the patients were divided into two groups, LFR-impaired group ( n=11, ICG R15≥20%) and control group ( n=162, ICG R15 < 20%). VTQ, FIB-4, platelet count and other parameters were compared between two groups. The multivariate logistic regression model was used to establish a risk model to access the impaired LFR. Receiver operating characteristic (ROC) curve was used to analyze the efficacy of each parameter in LFR-impaired. Results:The platelet count in LFR-impaired group was lower than that in control group, VTQ and FIB-4 were higher than that in control group (all P<0.05). Logistic regression showed that VTQ ( OR=4.382, 95% CI: 1.380-13.918)) and FIB-4 ( OR=2.112, 95% CI: 1.342-3.325) were risk factors for LFR-impaired. The final prediction model of LFR-impaired group was Logit (P)=-6.185+ 0.748×FIB-4+ 1.477×VTQ. The cut-off point (sensitivity, specificity, accuracy) of logistic model, FIB-4 and VTQ were 0.098 (72.8%, 90.1%, 89.0%), 0.990 (90.9%, 79.0%, 79.8%) and 1.8 m/s (81.8%, 77.8%, 78.0%), respectively. The specificity, accuracy of logistic model was higher than FIB-4 or VTQ. Conclusions:Logistic regression model based on VTQ and FIB-4 may improve the specificity and accuracy in the diagnosis of significant LFR impairment. VTQ can further assist clinicians in preoperative evaluation of LFR.

8.
Article in Chinese | WPRIM | ID: wpr-909591

ABSTRACT

OBJECTIVE To study the effect of polygonatum polysaccharide on zebrafish with Alzheimer disease. METHODS Zebrafish were trained in T maze for 7 d. The 40 zebrafish successfully trained were divided into 4 groups:blank group, model group, positive group and polygonatum polysaccharide group. Model group, positive group and polygonatum polysaccharide group were put in AlCl3100μg·L-1 for 6 d. The positive group was exposed to Huperzine A solution 4μg·L-1, and the polygonatum polysaccharide group was exposed to polygonatum polysaccharide solution 6 g·L-1 for 6 d. The model group was not treated, and the blank group was not treated. Each stage of zebrafish was recorded by video, and the time of each group in the EC region was analyzed. After administration, the brain tissue was taken out and the expression of N-cadherin, P38 and p-P38 protein factors was determined by Western blotting. RESULTS In behavior, the analysis of the time spent in the EC area, the blank group, the positive group and the polygonatum polysac?charide group were compared with the model group, respectively, there were statistically significant differences (P<0.05). At the protein level, compared with the model group, the P38 and p-P38 proteins in the positive group and the polygonatum polysaccharide group were down-regulated, while the N-cadherin protein was up-regulated, with statistical difference (P<0.05). CONCLUSION Polygonatum polysaccharide can improve the learning and memory ability of zebrafish with Alzheimer disease by up regulating the protein level of N-cadherin and hindering P38 phosphorylation.

9.
Article in Chinese | WPRIM | ID: wpr-906181

ABSTRACT

Objective:To observe the regulatory effect of modified Erzhu Erchentang on metabolization of polycystic ovary syndrome (PCOS) with spleen deficiency and phlegm dampness syndrome. Method:Patients 140 cases were divided into control group and observation group. Both groups were given metformin hydrochloride tablets, 500 mg/time, 3 times/day. Control group was given Yuejun Erchen pills, 0.5 g/time, 3 times/day, while observation group was given modified Erzhu Erchentang, 1 dose/day. The course of treatment lasted for 24 weeks. Before and after treatment, levels of fasting blood glucose (FBG), fasting insulin (FINS), glycosylated hemoglobin Alc (HbA1c), 2-hour postprandial blood glucose (2 h PG), blood lipid, waist circumference (WC), body mass index (BMI), waist hip ratio (WHR), luteinizing hormone (LH), follicle stimulating hormone (FSH), serum testosterone (T), estradiol (E<sub>2</sub>), dehydroepiandrosterone sulfate (DHEAS), sex hormone binding globulin (SHBG), leptin (LP), adiponectin (APN), resistin, visfatin and tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>) were detected. Homeostasis model assessment insulin resistance (HOMA-IR) was calculated, modified Erzhu Erchentang was scored, and recovery of menstruation and ovulation and ovarian volume were recorded. Result:Levels of FBG, 2 h PG, HbA1c, FINS, HOMA-IR, triglyceride (TG), total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), LH, FSH, T, E<sub>2</sub>, DHEAS, LP, resistin, visfatin and TNF-<italic>α</italic> in observation group were lower than those in control group (<italic>P</italic><0.01), and levels of BMI, WC and WHR were lower than those of control group (<italic>P</italic><0.05). And levels of high-density lipoprotein cholesterol (HDL-C), SHBG and APN were higher than those in control group (<italic>P</italic><0.01). Score of modified Erzhu Erchentang was lower than that in control group (<italic>P</italic><0.01), and ovarian volume was smaller than that in control group (<italic>P</italic><0.01). The normal rate of BMI was 49.23% (32/65), which was higher than 30.30% (20/66) in control group (<italic>χ</italic><sup>2</sup>=5.151, <italic>P</italic><0.05). The normal rate of blood lipid was 93.85% (61/65), which was higher than 81.82 % (54/66) in control group (<italic>χ</italic><sup>2</sup>=4.418, <italic>P</italic><0.05). The normal rate of blood glucose was 96.92% (63/65), which was higher than 86.36% (57/66) in control group (<italic>χ</italic><sup>2</sup>=4.474, <italic>P</italic><0.05). Conclusion:In addition to adipocytokines, modified Erzhu Erchentang could regulate adipokines of patients of PCOS with spleen deficiency and phlegm dampness, improve glucose, lipid metabolism and overweight, adjust endocrine hormone, reduce clinical symptoms and improve ovarian structure, so as to create conditions for conception.

10.
Article in Chinese | WPRIM | ID: wpr-905871

ABSTRACT

Objective:To investigate the effect of Qixian Tongluo prescription on neural function recovery in patients with cerebral infarction and its mechanism. Method:A total of 100 inpatients (January to June,2020)with cerebral infarction in the Neurology Department of Wenzhou Hospital of Traditional Chinese Medicine were assigned to an experimental group (<italic>n</italic>=50) and a control group (<italic>n</italic>=50) according to the random number table. Both groups received conventional treatment of western medicine,while the experimental group took additional Qixian Tongluo prescription. Treatment lasted for 12 weeks. The clinical efficacy,National Institutes of Health Stroke Scale (NIHSS) score, the modified Barthel index (MBI),Fugl-Meyer assessment (FMA) score, and levels of brain-derived neurotrophic factor(BDNF),vascular endothelial growth factor(VEGF), and stromal cell-derived factor-1(SDF-1) in peripheral blood of the two groups before and after treatment were compared. Result:The total response rate in the experimental group was 84.00%(42/50),higher than 66.00%(33/50) in the control group (<italic>Z</italic>=-7.365,<italic>P</italic><0.05). There was no significant difference in the scores of MBI,FMA, and NIHSS before treatment between the two groups. The MBI and FMA scores of the two groups increased (<italic>P</italic><0.01), and the NIHSS scores decreased (<italic>P</italic><0.05, <italic>P</italic><0.01). Compared with the control group after treatment, the experimental group showed increased MBI and FMA scores and decreased NIHSS score (<italic>P</italic><0.05). There was no significant difference in BDNF level between the two groups before and after treatment. The VEGF and SDF-1 levels in the peripheral blood of the two groups were higher than those before treatment (<italic>P</italic><0.05), and the experimental group was higher than the control group (<italic>P</italic><0.05). Conclusion:Qixian Tongluo prescription can effectively improve the clinical efficacy,the quality of life, and the prognosis of patients with cerebral infarction during convalescence. The underlying mechanism is associated with the promotion of the expression of endogenous VEGF and SDF-1 in the peripheral blood to activate the SDF-1/chemokine receptor 4(CXCR4) signaling pathway, induce the recruitment and mobilization of endothelial progenitor cells, and facilitate the angiogenesis and repair of ischemic brain tissues.

11.
Article in Chinese | WPRIM | ID: wpr-905821

ABSTRACT

Objective:Phthalates (PAEs) are common environmental endocrine disruptors. In this study, the effects of oxidative stress on liver and nutrient metabolism were determined in male diabetic rats exposed to di-2-ethylhexyl phthalate (DEHP), and the mechanism of DEHP toxicity was explored. Methods:Thirty-two SPF male Wistar rats aged five weeks, weighing 150-170 g, were fed adaptively for one week to establish the model of type 2 diabetes. The model was established by intraperitoneal injection of streptozotocin (STZ) (25 mg/kg) after feeding with high sugar and high fat diet for four weeks. Second STZ injection was given two days later. The model was considered to be established successfully when the random blood glucose level was found to be higher than 16.7 mmol/L in two separate tests. Twenty diabetic rats were then randomly divided into four groups, including control group (corn oil), 100, 300 and 900 mg/kg DEHP groups. The rats were treated with DEHP by gavage (5 mL/kg) once a day for 30 days. They were fed with normal diet during the treatment period. Caudal venous blood was collected on the 1st, 14th, and 28th days to measure the random blood glucose level. The changes of glucose tolerance were determined by oral glucose tolerance test on the 29th day. Fasting blood glucose (FPG) was measured on the next day of the last exposure. After the rats were anesthetized with pentobarbital and killed, the liver was weighed, the liver coefficient was calculated and the liver pathological section was made. Blood was taken from the abdominal aorta. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), triacylglycerol (TG) and albumin (ALB) in serum were measured by spectrophotometry, and the levels of insulin, glutathione (GSH), H2O2, malondialdehyde (MDA) and superoxide dismutase (SOD) in fasting serum were measured by radioimmunoassay. Results:There was no significant difference in body weight and random blood glucose in the type 2 diabetic rats exposed to different concentrations of DEHP (all P>0.05). At each time point of the glucose tolerance curve, the blood glucose value of the exposure groups was higher than that of the control group. A "false plateau period" appeared after the blood glucose value reached or exceeded the upper limit at 15 minutes, and the blood glucose level in each group was higher than that of the control group at 120 minutes. The liver organ coefficient of 300 and 900 mg/kg DEHP groups was higher than that of the control group (both P<0.01), and the liver organ coefficient was positively correlated with the exposure concentration of DEHP (r=0.80,P<0.000 1). Under the microscope, the liver cells in diabetic rats were swollen, the cytoplasm was light stained, and there were vacuoles in the cells. The serum ALP level in diabetic rats of 900 mg/kg DEHP group was significantly higher than that in the control group (P<0.01). The serum ALP level was positively correlated with the concentration of DEHP (r=0.75, P<0.01). The serum MDA level in diabetic rats of 300 mg/kg and 900 mg/kg DEHP groups was significantly higher than that of the control group (both P<0.01), and the serum MDA level was positively correlated with the concentration of DEHP (r=0.84, P<0.000 1). The serum SOD level of 900 mg/kg DEHP group was significantly higher than that of control group (P<0.01). Conclusion:DEHP exposure could lead to liver damage, abnormal glycolipid metabolism, and increase the level of oxidative stress and antioxidant level in male diabetic rats, but did not show a significant effect on insulin resistance.

12.
Article in Chinese | WPRIM | ID: wpr-905315

ABSTRACT

Objective:To observe the adhesion, growth and differentiation of rat neural stem cells (NSCs) on spinal cord acellular scaffold (SCAS) to evaluate its feasibility for spinal cord tissue engineering. Methods:NSCs derived from neonatal Sprague-Dawley rat cerebral cortex were cultured and identified. SCAS were prepared from female Sprague-Dawley rat spinal cord tissues using modified chemical extraction and physical oscillation, and evaluated. The third generation NSCs were planted on SCAS and co-cultured, the morphology of the cells on the scaffold was observed with immunofluorescence, immunohistochemistry and scanning electron microscope. Results:The cultured cells were NSCs, which could proliferate and differentiate. The porosity, water content and enzymatic hydrolysis rates of the prepared SCAS were significantly higher than that of normal spinal cord (|t| > 4.679, P < 0.01). The matrix structure of SCAS was loosely network-like, with few residual nuclei. NSCs adhered and grew well, and differentiated into neurons and glial cells on SCAS. Conclusion:This kind of SCAS shapes multi-channel spatial structure and is suitable for NSCs adhesion, growth and differentiation, which can be used for spinal cord tissue engineering.

13.
Article in Chinese | WPRIM | ID: wpr-905227

ABSTRACT

Objective:To explore the problems of seed cells and biological scaffolds in spinal cord tissue engineering, and review the recent experimental research. Methods:Related literatures were searched in CNKI, Wangfang data, PubMed and Web of Science from establishment to March, 2021, and the problems and progress of seed cells, biological scaffolds and their combination were reviewed. Results:The problems of seed cells are carcinogenicity, immune rejection, ethics, low survival rate and differentiation rate after transplantation, and current researches focus on exploring new cell types, gene transfection, cell co-transplantation and pretreatment before transplantation. The problems of biological scaffold are that a single material selection cannot meet different needs, and the traditional technology cannot simulate the internal structure of spinal cord well. There were more researches focusing on new composite materials and new technology. The core problem of their combination is that the effects of different cell and scaffold combinations are different, and the current researches are mostly devoted to the continuous exploration of suitable composite mode, and try to introduce biological agents and other factors. Conclusion:Spinal cord tissue engineering has the potential to completely change the therapeutic pathway of spinal cord injury. Current experimental researches mainly base on solving the problems of seed cells and biological scaffolds of spinal cord tissue engineering, and further explore the appropriate composite mode of seed cells and biological scaffolds, so as to provide more basic evidence for its clinical application.

14.
Article in English | WPRIM | ID: wpr-880988

ABSTRACT

OBJECTIVE@#Traditional Chinese medicine plays a significant role in the treatment of the pandemic of coronavirus disease 2019 (COVID-19). Tanreqing Capsule (TRQC) was used in the treatment of COVID-19 patients in the Shanghai Public Health Clinical Center. This study aimed to investigate the clinical efficacy of TRQC in the treatment of COVID-19.@*METHODS@#A retrospective cohort study was conducted on 82 patients who had laboratory-confirmed mild and moderate COVID-19; patients were treated with TRQC in one designated hospital. The treatment and control groups consisted of 25 and 57 cases, respectively. The treatment group was given TRQC orally three times a day, three pills each time, in addition to conventional Western medicine treatments which were also administered to the control group. The clinical efficacy indicators, such as the negative conversion time of pharyngeal swab nucleic acid, the negative conversion time of fecal nucleic acid, the duration of negative conversion of pharyngeal-fecal nucleic acid, and the improvement in the level of immune indicators such as T-cell subsets (CD3, CD4 and CD45) were monitored.@*RESULTS@#COVID-19 patients in the treatment group, compared to the control group, had a shorter negative conversion time of fecal nucleic acid (4 vs. 9 days, P = 0.047) and a shorter interval of negative conversion of pharyngeal-fecal nucleic acid (0 vs. 2 days, P = 0.042). The level of CD3@*CONCLUSION@#Significant reductions in the negative conversion time of fecal nucleic acid and the duration of negative conversion of pharyngeal-fecal nucleic acid were identified in the treatment group as compared to the control group, illustrating the potential therapeutic benefits of using TRQC as a complement to conventional medicine in patients with mild and moderate COVID-19. The underlying mechanism may be related to the improved levels of the immune indicator CD3


Subject(s)
Adult , Antiviral Agents/therapeutic use , COVID-19/pathology , Capsules , DNA, Viral/analysis , Drugs, Chinese Herbal/therapeutic use , Feces/virology , Female , Humans , Length of Stay , Lymphocyte Count , Male , Medicine, Chinese Traditional/methods , Middle Aged , Retrospective Studies , SARS-CoV-2/genetics , Severity of Illness Index , Treatment Outcome , Young Adult
15.
Article in English | WPRIM | ID: wpr-880527

ABSTRACT

Cupping therapy has been accepted worldwide, and many studies have been conducted to reveal its curative effects and mechanisms. To comprehensively evaluate the effect of cupping therapy, database including China Network Knowledge Infrastructure (CNKI), Chinese Scientific Journal Database VIP, Wan Fang Database, Chinese Biomedicine (CBM), PubMed and Web of Science were searched from 2009-2019. We summarized all the meta-analyses, randomized controlled trials, clinical trials and the mechanisms studies of cupping therapy in the previous 10 years, hoping to provide a reference for the clinical applications and studies.

16.
Article in English | WPRIM | ID: wpr-921395

ABSTRACT

OBJECTIVES@#To study the effect of preoperative prophylactic use of antibiotics on wound infection in patients with cleft lip.@*METHODS@#Aretrospective study was conducted on the clinical data of 1 361 patients who underwent one-stage cleft lip repair in the Department of Cleft Lip and Palate in West China Hospital of Stomatology, Sichuan University, from January 2015 to November 2018. The patients were divided into two groups according to whether prophylactic antibiotics were used or not. There were 594 patients in the prevention group, including 373 unilateral incomplete cleft lip (UICL) patients, 157 unilateral complete cleft lip (UCCL) patients, 25 bilateral incomplete cleft lip (BICL) patients, 39 bilateral complete cleft lip (BCCL) patients. There were 767 patients in the non-prophylactic group, including 482 UICL patients, 211 UCCL patients, 31 BICL patients, 43 BCCL patients. The relationship between preoperative and postoperative leukocyte count, preoperative and postoperative body temperature, and postoperative wound infection were compared between the two groups.@*RESULTS@#No significant difference was observed in the leukocyte count and body temperature between both groups (@*CONCLUSIONS@#The preoperative prophylactic use of antibiotics has no significant effect on the incidence of postoperative infection in patients undergoing cleft lip repair.


Subject(s)
Anti-Bacterial Agents , Cleft Lip/surgery , Cleft Palate/surgery , Humans , Infant , Surgical Wound Infection/prevention & control
17.
Chinese Acupuncture & Moxibustion ; (12): 1089-1094, 2021.
Article in Chinese | WPRIM | ID: wpr-921015

ABSTRACT

OBJECTIVE@#To compare the clinical therapeutic effect between deep needling at Xiaguan (ST 7) with round sharp needle combined with plum-blossom needle and conventional acupuncture in patients with trigeminal neuralgia (TN) of wind and heat, and explore its mechanism.@*METHODS@#A total of 60 patients with TN of wind and heat were randomized into an observation group (30 cases) and a control group (30 cases). In the observation group, deep needling with round sharp needle was applied at Xiaguan (ST 7), and tapping with plum-blossom needle was applied at Yangbai (GB 14), Quanliao (SI 18), Dicang (ST 4), Sibai (ST 2), etc. of affected side. In the control group, conventional acupuncture was applied at the same acupoints selected in the observation group. The treatment was given once a day, 5 times a week for 4 weeks in the both groups. Before and after treatment, the scores of short-form McGill pain questionnaire (SF-MPQ), TCM syndrome, patient global impression of change (PGIC) and comprehensive symptom were observed, the serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), vasoactive intestinal peptide (VIP) and β-endorphin (β-EP) were detected, and the adverse reaction was observed in the both groups.@*RESULTS@#After treatment, the scores of PRI, PPI, VAS, TCM syndrome, PGIC and comprehensive symptom and the serum levels of IL-6, TNF-α and VIP were decreased compared before treatment in the both groups (@*CONCLUSION@#Deep needling at Xiaguan (ST 7) with round sharp needle combined with plum-blossom needle can effectively treat the trigeminal neuralgia of wind and heat and relieve pain, its therapeutic effect is superior to conventional acupuncture. The mechanism may be related to the regulation of serum IL-6, TNF-α, VIP and β-EP.


Subject(s)
Flowers , Hot Temperature , Humans , Prunus domestica , Trigeminal Neuralgia/therapy , Wind
18.
Chinese Journal of Biotechnology ; (12): 1637-1658, 2021.
Article in Chinese | WPRIM | ID: wpr-878659

ABSTRACT

Filamentous fungi are important industrial microorganisms that play important roles in the production of bio-based products such as organic acids, proteins and secondary metabolites. The development of metabolic engineering and its enabling techniques have greatly promoted the design, construction and application of filamentous fungal cell factories. This article systematically reviews the development of filamentous fungal cell factories constructed through metabolic engineering, and discusses the challenges and future perspectives for systems metabolic engineering of filamentous fungi.


Subject(s)
Fungi/genetics , Metabolic Engineering
19.
Chinese Journal of Biotechnology ; (12): 680-688, 2021.
Article in Chinese | WPRIM | ID: wpr-878593

ABSTRACT

In the era of Internet +, teaching models in universities are undergoing changes due to the rapid development of information technology. Blended teaching, combining online with offline teaching, is being implemented and developed in universities. In order to reform teaching mode and improve teaching effect, the curriculum team carried out the exploration of blended teaching reform for the "Introduction to Life Sciences" for non-biology students. The course combined high-level MOOC (Massive Open Online Course), small class teaching, diversified platform and multi-dimensional teaching mode, built a multi-disciplinary collaborative teaching team, formed a multi-dimensional evaluation system focusing on process and ability, practiced the education concept of combining knowledge teaching and value leading, gained valuable practical experience, and achieved the expected teaching results. It can provide reference for the reform and construction of similar courses in other colleges and universities. The development of blended teaching expands the breadth and depth of teaching, stimulates students' interest and potential for learning, opens up students' thinking and perspective, cultivates students' scientific literacy and comprehensive ability, and plays a positive role in the cultivation of innovative and inter-disciplinary talents.


Subject(s)
Biological Science Disciplines , Curriculum , Humans , Learning , Students , Universities
20.
Article in English | WPRIM | ID: wpr-878410

ABSTRACT

OBJECTIVES@#This study aimed to investigate the association between periodontal indexes and biomarkers in gingival crevicular fluid (GCF) and preterm birth (PTB) in pregnancy, as well as to assess the clinical value of these indexes as predictors of PTB.@*METHODS@#A nested case-control study was conducted. A total of 300 systematically healthy pregnant women were selected within 36 weeks of gestation and grouped according to the enrolled weeks. Periodontal indexes, including probing depth (PD), bleeding index (BI), gingival index (GI), and five biomarkers in GCF, including interleukin (IL)-1β, IL-6, tumor necrosis factor-α (TNF-α), prostaglandin E2 (PGE2), and 8-hydroxy-2-deoxyguanosine (8-OHdG) were measured at the enrolled date. The detailed birth outcome was recorded.@*RESULTS@#Only women at 24-28 weeks of gestation per PTB case (four full-term births) were selected as controls subjects, PTB displayed significantly greater GI, BI, and 8-OHdG (@*CONCLUSIONS@#Increased BI and 8-OHdG at 24-28 weeks of gestation are risk factors for PTB. Their combined detection may have some value in the prediction of PTB, but further studies with a larger sample size are needed to explore it and thus provide experiment evidence for establishing an early warning system for PTB in pregnant women with periodontal disease.


Subject(s)
Biomarkers , Case-Control Studies , Female , Gingival Crevicular Fluid , Humans , Infant, Newborn , Periodontal Index , Pregnancy , Premature Birth
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