Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 761
Filter
1.
Article in Chinese | WPRIM | ID: wpr-879418

ABSTRACT

OBJECTIVE@#To measure the maximum corridor parameters of the infra acetabular screw and evaluate the feasibility of screw insertion through digital analysis of the acetabular structure.@*METHODS@#The pelvic CT data of 100 patients who received plain pelvic CT scan from April 2013 to June 2015 were retrospectively analyzed. There were 50 males, aged 20 to 84 years, with an average age of (48.42±17.48) years, and 50 females, aged 18 to 87 years, with an average age of (55.02±19.54) years. Patients with acetabular fractures, hip dysplasia, and metal implants in the acetabulum were excluded. Import CT data into Mimics software in DICOM format to generate a three-dimensional model, and find the axialprojection of the infra-acetabular corridor in the middle of the pubis ramus in the inlet view. A virtual screw was placed in the infra-acetabular space and measure the parameters including the diameter and the length of the maximum corridor, the distance from the insertion point to the pubic symphysis, to the anterosuperior iliac spine and to the medial edge of the pelvis. Then import the pelvic model into 3- matic software, establish the pelvic model anterior pelvic plane and median sagittal plane, and measure the angle between the screw axis and the two planes. A minimum corridor diameter of at least 5 mm was defined as a cutoff for placing a 3.5 mm screw, and calculate the screw insertion rate.@*RESULTS@#In 100 cases, 49% of patients had a infra acetabular corridor with a diameter ≥5 mm, and the rate of screw placement in men was significantly higher than that in women. The average diameter of the maximum corridor of infra-acetabular screw was (4.86±1.72) mm, the average length was (94.04±8.29) mm, the average distance from the insertion point to the pubic symphysis was (60.92±4.84) mm, to the anterosuperior iliac spine was (85.15± 6.85) mm, and to the medial edge of the pelvis was (6.12±3.32) mm. The mean angle between the axis of the screw and the median sagittal plane was (-1.38±4.74)°, and the mean angle between the axis of the screw and the anterior pelvic plane was (56.77±7.93)°. There are significant differences between male and female measured parameters, except for the angle between the screw axis and the anterior pelvic plane. There was no statistically significant difference in the maximum corridor parameters of infra-acetabular screw on both sides of the pelvis.@*CONCLUSION@#This study shows that the insertion rate of infra-acetabular screws is low in local patients, and the feasibility of screw insertion should be fully evaluated before surgery.


Subject(s)
Acetabulum/surgery , Adolescent , Adult , Aged , Aged, 80 and over , Bone Screws , Feasibility Studies , Female , Fracture Fixation, Internal , Humans , Male , Middle Aged , Retrospective Studies , Young Adult
2.
Article in English | WPRIM | ID: wpr-914443

ABSTRACT

Background@#The purpose of this study was to identify the association between participation in social activities and mortality rates for those aged 45 aged and older in Korea. @*Methods@#In this study, the 1st to 6th Korea Longitude Study of Aging was used to analyze 10,217 people excluding missing values among middle and old age groups aged 45 or older. The scope of social activities was classified into “religious gatherings,” “religious gatherings,” “leisure/cultural/sports-related organizations,” and “clubs/hometowns/religious associations,” and analyzed using a chi-square test and Cox proportional risk model. @*Results@#In the case of non-participating groups in religious activities, the mortality rate was 1.24 times higher (hazard ratio [HR], 1.24; p=0.000) than those of the participating group. The non-participating group of social gatherings had a 1.27 times higher mortality rate (HR, 1.27; p<0.0001) than the participating group. In addition, the mortality rate of non-participating groups related to leisure/cultural/sports was 1.79 times higher (HR, 1.79; p=0.000). The mortality rate of the group that did not participate in the alumni association/festival/folklore society was 1.51 times higher than that of the participating group (HR, 1.51; p<0.0001). As a result of correcting the control variable to analyze the relationship between the number of participants in social activities and the mortality rate, the mortality rate of the group participating in one or less social activities was 2.26 times higher (HR, 2.26; p<0.0001) compared to the four or more social activity participating groups, and the mortality rate of the 1–3 social activities was 1.64 times higher (HR, 1.64; p<0.0001). @*Conclusion@#As a result of the study, it was found that participation in social activities of the middle-aged and elderly groups was effective in reducing mortality, and in particular, it was found that there was a strong relationship with mortality in less than one social activity group. Therefore, it is intended to provide an academic basis for lowering the mortality rate of the group in line with the continuous improvement of domestic social activity participation conditions, and through this, this study can be expected to serve as a policy and institutional basis for lowering the mortality rate of the group.

3.
Article in Chinese | WPRIM | ID: wpr-912011

ABSTRACT

Objective:To observe the effect of electroacupuncture at the Baihui acupoint on learning and memory ability and on the calmodulin kinase (CaMK)Ⅱ-Tau protein signal pathway in rats exposed to infrasound, and to explore its mechanism.Methods:Forty-eight Sprague-Dawley rats were randomly divided into a blank group, an infrasound group, a Baihui group and a non-acupoint group, each of 12. The rats in the blank group were placed in an infrasound chamber without infrasound for 2 hours daily. Those in the other 3 groups were exposed to 8Hz, 130dB infrasound in the chamber for 2 hours daily for 7 consecutive days. The rats in the Baihui and non-acupoint groups were given electroacupuncture within 2 hours after the infrasound exposure at the Baihui acupoint or elsewhere respectively. The rats in the blank and infrasound groups were given the same grasping and fixation, but no electroacupuncture. On the 6th and 7th day of intervention, Morris water maze positioning and navigation experiments and spatial exploration experiments were used to quantify the rats′ spatial learning and memory ability. Nissl staining was used to observe any changes in the morphology of the neurons in the hippocampus of 6 rats in each group. The expression of phosphorylated calmodulin-dependent protein kinase II (P-CaMKⅡ) and phosphorylated Tau protein (P-Tau) in the hippocampus was also documented using western blotting.Results:After 6 or 7 days the average escape latency of the rats in the infrasound group was significantly longer than the blank group′s average. Platform quadrant time and distance ratios and the number of times crossing the platform area were also significantly lower. Compared with the infrasound group, the average escape latency of the Baihui group was significantly shorter, with the platform quadrant time and distance ratios and the number of times crossing the platform area significantly higher. After 7 days, the damage to hippocampal neurons among the rats in the infrasound group was significantly aggravated and the number of neurons was reduced significantly compared with the blank group. Compared with the infrasound group, significantly fewer neurons in the hippocampus were damaged in the Baihui group and the number of neurons had increased significantly. After the intervention the levels of P-CaMKⅡand P-Tau protein in the infrasound group had increased significantly compared with the blank group, but those levels in the Baihui group were significantly lower, on average.Conclusion:Electroacupuncture at the Baihui acupoint can improve the learning and memory ability of rats exposed to infrasound, and has some protective effect against infrasound brain damage. That may be due to its inhibiting Tau protein hyperphosphorylation in the hippocampus by reducing CaMKⅡ activity.

4.
Chinese Journal of Dermatology ; (12): 738-741, 2021.
Article in Chinese | WPRIM | ID: wpr-911517

ABSTRACT

Imatinib mesylate is the first-line drug for the treatment of chronic myeloid leukemia and malignant gastrointestinal stromal tumors, and causes mucocutaneous adverse reactions at a high frequency. Based on Chinese and international literature, the authors comprehensively describe various mucocutaneous symptoms induced by imatinib mesylate, and summarize their pathogenesis and management strategies, aiming to help dermatologists improve their understanding of mucocutaneous adverse reactions to this drug, and provide timely diagnosis and treatment.

5.
Article in Chinese | WPRIM | ID: wpr-911379

ABSTRACT

Objective:Based on knowledge visualization analysis to explore the progress of diabetic cardiomyopathy from 2000 to 2020.Methods:Literatures related to diabetic cardiomyopathy indexed in Web of Sciences databases ranging from 2000 to 2020 were collected. Excel, VOSviewer, and CiteSpace 5.0.R1 software were used for visual analysis of countries, research institutions, authors, keywords and related citations.Results:A total of 2 536 articles were collected preliminarily. China has published the most articles in this field through visualization. A total of 909 related articles have been published in the past 20 years, and China has been closely communicated internationally. The United States and Canada ranked the second and the third respectively, totaling 723 and 201. For the distribution of research institutions, the University of Louisville ranked first with 86 articles. A few top high-impact authors, such as Lu Cai and Tan Yi, are from this institution and have published the most articles, indicating that the University of Louisville can be regarded as an international academic center in the field of diabetic cardiomyopathy. Domestic Jilin University and Shandong University followed, ranking the second and the third with 70 and 66 papers respectively. Keyword co-occurrence analysis shows that research hotspots in this field at home and abroad mainly focus on the investigation of the clinical manifestations and pathogenesis of diabetic cardiomyopathy. Finally, through the co-cited literature network, cluster #4 (SGLT-2 inhibitor) is still in evolvement. The development process shows that the treatment is a new trend in the field of diabetic cardiomyopathy research.Conclusions:Knowledge visualization analysis can visually exhibit the intellectual structure, evolution process, hotspots and trending in the field of diabetic cardiomyopathy research. The results showed that the main research power was located in the China, but the cooperation among personnel needs to be strengthened. The future research trend is mainly focused on the treatment of diabetic cardiomyopathy.

6.
Chinese Journal of Radiology ; (12): 1301-1307, 2021.
Article in Chinese | WPRIM | ID: wpr-910296

ABSTRACT

Objective:To explore the value of quantitative susceptibility mapping (QSM) in evaluating renal injury in patients with early diabetic nephropathy (DN).Methods:From October 2019 to December 2020, 32 patients with early DN were prospectively enrolled in the Third Affiliated Hospital of Soochow University. According to the estimated glomerular filtration rate (eGFR), they were divided into three groups: DN1 (eGFR≥90 ml·min -1·1.73 m -2, 11 cases), DN2 (60-<90 ml·min -1·1.73 m -2, 11 cases) and DN3 (30-<60 ml·min -1·1.73 m -2, 10 cases). At the same time, 32 normal volunteers were recruited as the control group. Both kidneys were scanned by QSM to measure the susceptibility of renal cortex and medulla. Paired samples t-test was used to compare the differences of the susceptibility between left and right kidneys and between cortex and medulla. One-way analysis of variance was performed to compare the differences of corresponding susceptibility values among different groups, and LSD- t was used for the pairwise comparison. Pearson correlation test was performed between the susceptibility value of the medulla and eGFR. The ROC curve was used to analyze the diagnostic efficacy of QSM parameters in the diagnosis of DN and different degrees of severity of DN. Results:The susceptibility values of bilateral renal medulla in normal volunteers and patients with DN were lower than those of renal cortex (all P<0.001). There was no significant difference in the susceptibility value between left and right renal cortex (all P>0.05). There was significant difference in the susceptibility value between left and right medulla (all P<0.05). There was no significant difference in the susceptibility value of bilateral renal cortex among the control group and the DN1-DN3 groups (both P>0.05). The susceptibility values of left renal medulla in control group, DN1, DN2 and DN3 groups were (-4.46±1.16)×10 -2, (-5.96±0.97)×10 -2, (-7.97±1.25)×10 -2, (-9.58±1.45)×10 -2 ppm, of right renal medulla were (-3.70±0.65)×10 -2, (-5.06±1.28)×10 -2, (-7.33±1.46)×10 -2, (-9.09±2.22)×10 -2 ppm, respectively. The overall difference of the susceptibility value of bilateral renal medulla was statistically significant (both P<0.05), and there were significant differences between each two groups (all P<0.05). The linear positive correlation were found between the susceptibility values of renal medulla and the corresponding eGFR (left kidney r=0.732, P<0.001; right kidney r=0.684, P<0.001). The areas under the ROC curve (AUC) of left and right renal medulla susceptibility value in diagnosis of normal and DN were 0.931 and 0.943, of DN1 and DN (2 and 3) were 0.952 and 0.883, of DN (1 and 2) and DN3 were 0.888 and 0.831, respectively. Conclusion:The susceptibility value of QSM quantitative parameter has a certain value in the staging and differential diagnosis of early DN, among which the susceptibility value of renal medulla has higher diagnostic efficiency.

7.
Chinese Critical Care Medicine ; (12): 529-534, 2021.
Article in Chinese | WPRIM | ID: wpr-909353

ABSTRACT

Objective:To evaluate the effects of noise, bright light and mechanical stimulation on sleep, blood-brain barrier and cognitive function in septic rats.Methods:Forty male Sprague-Dawley (SD) rats were selected and intraperitoneal injection of 10 mg/kg lipopolysaccharide (LPS) was used to establish sepsis model. 0, 30, 45, 60, 75 dB noise stimulation or 0, 50, 100, 200, 400 Lux light stimulation were given to rats (all n = 4). The serum levels of cortisol and melatonin, and the cerebral content of Evans blue (EB) were measured 96 hours after the stimulation to determine the optimal intensity of intervention. The other 40 SD rats were randomly divided into control group (Con group), LPS group, noise intervention group (LPS+60 dB group), 200 Lux light intervention group (LPS+200 Lux group) and mechanical stimulation group (LPS+MS group), with 8 rats in each group. The open fields test and fear conditioning test were used to evaluate the exploratory behavior and cognitive function 96 hours after corresponding stimulation. The enzyme linked immunosorbent assay (ELISA) was used to detect cerebral level of interleukin-6 (IL-6) and serum levels of cortisol and melatonin. The blood-brain barrier integrity was assessed by EB staining. The protein levels of ZO-1, Claudin-5 and caspase-3 in the hippocampus were detected by Western blotting to assess the blood-brain barrier integrity and neuronal apoptosis. Results:Compared with 0 dB group or 0 Lux group, the serum melatonin concentration in 60 dB group and 200 Lux group were significantly reduced, while the serum cortisol concentration and cerebral EB content were significantly increased. Therefore, 60 dB noise and 200 Lux light were selected in the subsequent experiments. Compared with Con group, the horizontal score and vertical score in the open field test in LPS group were significantly decreased. There were no significant differences in the proportion of freezing time, the cerebral contents of EB and IL-6, the serum levels of melatonin and cortisol, and the hippocampal expressions of ZO-1, Claudin-5 and caspase-3. Compared with LPS group, the horizontal score, vertical score and the percentage of freezing time in LPS+60 dB group, LPS+200 Lux group and LPS+MS group were significantly reduced [horizontal score: 73.8±9.7, 80.3±9.4, 64.5±8.3 vs. 103.6±15.5; vertical score: 9.4±1.7, 11.2±1.9, 6.8±0.9 vs. 15.9±2.8; the percentage of freezing time: (45.3±4.7)%, (53.3±5.8)%, (42.1±5.1)% vs. (66.1±6.3)%], the serum level of melatonin was significantly decreased (ng/L: 53.62±6.20, 44.25±6.41, 45.33±5.84 vs. 74.39±7.54), the serum level of cortisol was significantly increased (nmol/L: 818.34±95.53, 710.04±65.41, 989.73±91.63 vs. 398.82±72.59), the levels of EB, IL-6 in the brain tissue were significantly increased [EB (μg/g): 2.80±0.35, 2.38±0.31, 3.24±0.42 vs. 1.59±0.26; IL-6 (ng/g): 31.56±4.11, 26.69±3.75, 37.47±4.56 vs. 16.28±2.69], the expressions of ZO-1 and Claudin-5 were significantly decreased (ZO-1/β-actin: 0.37±0.04, 0.32±0.05, 0.24±0.04 vs. 0.80±0.09; Claudin-5/β-actin: 0.62±0.08, 0.47±0.06, 0.35±0.05 vs. 0.97±0.20), and the expression of cleaved caspase-3 was significantly increased (caspase-3/β-actin: 0.56±0.06, 0.39±0.04, 0.72±0.12 vs. 0.20±0.03), with statistically significant differences (all P < 0.05). Conclusion:60 dB noise, 200 Lux light or mechanical stimulation for 96 hours could inhibit the secretion of serum melatonin, promote the secretion of cortisol, and activate neuroinflammation in septic rats, and lead to neuronal apoptosis in hippocampus and hyper-permeability of blood-brain barrier, which in turn could cause sleep disturbance and cognitive impairment.

8.
Chinese Journal of Endemiology ; (12): 902-905, 2021.
Article in Chinese | WPRIM | ID: wpr-909122

ABSTRACT

Objective:To investigate the arsenic content in internal and external environment of the people in drinking-water-borne endemic arsenic poisoning areas in Shaanxi Province, the status quo of the disease and the management and services of the existing patients, so as to provide a theoretical basis for evaluating the effect of prevention and control measures on drinking-water-borne endemic arsenic poisoning.Methods:From 2019 to 2020, in accordance with the requirements of the "Notice of Shaanxi Provincial Health Commission on Issuance of Monitoring Plans for Key Endemic Diseases such as Kashin-Beck Disease" and "Monitoring Plans for Drinking Water Arsenic Poisoning in Shaanxi Province", investigation was carried out in all villages of drinking-water-borne endemic arsenic poisoning in Mianxian County of Hanzhong City, Shanyang County and Zhen'an County of Shangluo City to understand the operation of water improvement project. One terminal water sample was collected to determine the content of arsenic in water, and permanent population of the whole village who are and/or had been exposed to excessive arsenic water in the past were examined for arsenic poisoning and the disease condition was determined according to the standard of "Diagnosis of Endemic Arsenicosis" (WS/T 211-2015). Five villages were randomly selected, and 50 people were selected from each village. Real-time urine sample was collected to determine the content of urinary arsenic. At the same time, management and services of the existing arsenic poisoning patients was investigated.Results:A total of 13 villages were investigated, all of which had been improved in water quality, and all 13 water improvement projects were operating normally, with arsenic content < 0.01 mg/L. A total of 12 577 people were examined, and 417 cases of arsenic poisoning were detected, with a detection rate of 3.32%. No new arsenic poisoning case and arsenic-related skin cancer case had been detected. A total of 260 urine samples were detected, and the geometric mean value of urinary arsenic (< 0.001 mg/L) was lower than the safety guideline value of human urinary arsenic (0.032 mg/L). The existing arsenic poisoning patients (417 cases) were included in health management and contracted services.Conclusions:Shaanxi Province has reached the elimination standard of drinking-water-borne endemic arsenic poisoning. In the future, more attention should be paid to the management of water improvement project, as well as to strengthen health promotion and management services for existing patients to consolidate the effects of prevention and control.

9.
Article in Chinese | WPRIM | ID: wpr-908216

ABSTRACT

Objective:To explore the effect of staged respiratory training in pulmonary rehabilitation nursing of patients with high spinal cord injury.Methods:A total of 76 patients with high spinal cord injury in the Second Affiliated Hospital of Air Force Military Medical University from January 2018 to December 2019 were selected as the study subjects, and were divided into the control group and the observation group with random number table method, 38 cases each. The control group was given conventional respiratory training, while the observation group was given staged respiratory training. Both groups were intervened 6 days a week for 8 weeks. The differences of basic indexes, pulmonary ventilation function, respiratory muscle strength and incidence of pulmonary infection between the two groups before and after intervention were compared.Results:After 8 weeks of intervention, the blood oxygen saturation was 0.95±0.04 in the observation group and 0.90±0.04 in the control group, there was significant difference ( t value was 4.229, P<0.001). The percentages of predicted values of maximal vital capacity, forced expiratory vital capacity, forced expiratory volume in 1s, and maximal ventilation were (69.21±11.38)%, (61.83±11.53)%, (56.50±7.86)%, (51.62±8.73)% in the observation group after 8 weeks of intervention, and (56.70±14.65)%, (49.82±15.06)%, (45.61±10.32)%, (42.30±15.11)% in the control group, there was significant difference ( t values were 3.610-4.967, P<0.001). The inspiratory muscle strength index was (56.12±18.31) cmH 2O(1 cmH 2O= 0.098 kPa) in the observation group after intervention, and (46.61±11.62) cmH 2O in the control group, there was significant different ( t value was 2.806, P<0.01). The incidence of pulmonary infection was 5.26% (2/38) in the observation group and 15.78% (6/38) in the control group, there was no significant difference ( χ2 value was 2.235, P>0.05). Conclusions:Stage respiratory training can promote lung rehabilitation of patients with high spinal cord injury, which is worthy of promotion and application.

10.
Article in Chinese | WPRIM | ID: wpr-906528

ABSTRACT

Objective:To explore the reasonable combination of Artemisiae Annuae Herba and Chuanxiong Rhizoma in treatment of cerebral malaria and investigate its mechanism based on network pharmacology. Method:The traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP) and SymMap were used to obtain all the chemical components of Artemisiae Annuae Herba and Chuanxiong Rhizoma and the action targets were screened to construct a component target protein-protein interaction (PPI) network. Target genes related to cerebral malaria were collected with use of GeneCards and DisGeNET databases. Common targets were screened by overlapping drug targets and disease targets, and protein-protein interaction network analysis was performed to get key targets. Gene ontology (GO) functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were carried out to get main signaling pathways. Furthermore, the classical experimental cerebral malaria mouse model was used to detect survival curve, protozoanemia level, survival rate, experimental cerebral malaria (ECM) coma and behavior scores. RayBio<sup>®</sup> cytokine antibody array was used to detect the expression level of cytokines in tissues and experiment was conducted for verification. Result:After combination of Artemisiae Annuae Herba and Chuanxiong Rhizoma, 23 active ingredients, 179 drug targets, and a total of 100 common targets of the drug and disease were obtained. GO functional analysis identified 59 items (<italic>P</italic><0.05), involving cytokine activity, growth factor activity, immune response, etc. KEGG pathway analysis revealed 51 related signaling pathways. The experimental results showed that the combined use of Artemisiae Annuae Herba and Chuanxiong Rhizoma could significantly improve the clinical signs of ECM mice, such as survival state, coma and behavioral scores. In the detection of expression levels of related cytokines in mice, the expression levels of <italic>γ-</italic>interferon (IFN-<italic>γ)</italic>, interleukin-10 (IL-10), IL-4, and IL-1<italic>β</italic> in the compatible drug combination drug were significantly higher than those in the model group (<italic>P</italic><0.05), which was consistent with the overlapping core targets predicted by network pharmacology. Conclusion:Based on the network pharmacology analysis and<italic> in vivo</italic> experiment verification, this study confirmed the synergistic effect of the combination of Artemisiae Annuae Herba and Chuanxiong Rhizoma in the treatment of cerebral malaria, providing clear direction for further mechanism research, and a new possibility for the clinical intervention of cerebral malaria.

11.
Article in Chinese | WPRIM | ID: wpr-906522

ABSTRACT

Objective:To investigate the intervention of <italic>Hedyotis diffusa</italic> (HDW) on colitis associated cancer (CAC) model mice and explore its mechanism. Method:The CAC mouse model was established by synergistic action of azoxymethane (AOM) and dextran sulfate sodium (DSS). The intervention of HDW on CAC mice was evaluated by disease activity index (DAI), colonic tissue morphology, pathological injury score and tumorigenesis rate. Ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) and multivariate statistical analysis were used to analyze the metabonomics of mice serum and to explore the mechanism of HDW intervention on CAC. Result:HDW could significantly improve the general condition of CAC mice, decrease DAI, colon gross morphological score, histopathological score and tumorigenesis rate. Compared with the normal group, 38 kinds of differential metabolites were screened in the model group, including 11 potential biomarkers, involving 11 main metabolic pathways. HDW could significantly regulate 9 kinds of differential metabolites [niacinamide, uridine, 4-pyridoxic acid, LysoPC (18∶0), LysoPE (0∶0/20∶0), myo-inositol, purine, sphinganine 1-phosphate and tetradecanedioic acid] in the model group, including 2 kinds of potential biomarkers (myo-inositol and niacinamide), and HDW could regulate nicotinate and nicotinamide metabolism and inositol phosphate metabolism. Conclusion:HDW has a therapeutic effect on CAC, which may be achieved by regulation of energy metabolism and glucose metabolism.

12.
Article in Chinese | WPRIM | ID: wpr-906220

ABSTRACT

Objective:To analyze active components, its targets and signaling pathways of Shenlian formula based on network pharmacology, and explore the molecular mechanism of Shenlian formula in the treatment of atherosclerotic cardiovascular disease (ASCVD), in order to provide a basis for the rational interpretation of the prescription compatibility of Shenlian formula. Method:Major chemical compounds of the formula were obtained by SymMap and Systematic pharmacology database and analysis platform of Traditional Chinese Medicine (TCMSP), its target proteins were obtained by SymMap and ETCM Databases, and the pathogenic genes responsible for of ASCVD were obtained by DisGeNET and GEO Datebases. Protein targets of drugs and pathogenic genes of diseases were overlapped to obtain predicted targets of Shenlian Formula for ASCVD. Proteins-proteins interactions (PPI) network was built through the String Datebase. The Cytoscape 3.6.0 was used to explore the key compounds and targets of Shenlian formula on ASCVD. Then gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway were analyzed to screen out the key targets of Shenlian Formula. Rat I/R model was adopted as representative disease model of ASCVD for experimental verification. Result:There were 59 candidate compounds, 67 predicted targets and 29 key targets of Shenlian formula on ASCVD. Key targets mainly included cyclooxygenase 2 (PTGS2), estrogen receptor 1 (ESR1) and TP53. GO analysis showed that the biological functions of potential genes of Shenlian formula in treatment of ASCVD were mainly related to apoptotic, nitric oxide biosynthetic process, response to estradiol, angiogenesis, inflammatory response and oxidative stress and acute-phase response. KEGG pathway enrichment results showed that the pathways of potential genes of Shenlian formula in treatment of ASCVD mainly involved TNF signaling pathway, phosphatidylinositol-3 kinase (PI3K)/ protein kinase B (Akt) signaling pathway, hypoxia induction factor-1 (HIF-1) signaling pathway and apoptosis. Among them, the regulatory effect of Shenlian formula on apoptosis may act on not only TP53, but also different signaling pathways of apoptosis respectively, thus playing a synergistic effect. <italic>In vivo</italic> experimentation confirmed that Shenlian formula could significantly reduce the myocardial infarction area, improve the myocardial histopathological changes, and especially reduce myocardial mitochondrial injury. Further analysis showed that Shenlian formula can significantly inhibit the expressions of activated proteins in mitochondrial apoptosis pathway. Conclusion:Anti-atherosclerosis traditional Chinese medicine Shenlian formula could effectively intervene ASCVD, and its effect on mitochondrial apoptosis of myocardial cells is one of its mechanisms in protecting myocardial ischemia-reperfusion injury.

13.
Article in Chinese | WPRIM | ID: wpr-906142

ABSTRACT

Objective:To investigate the effect of Suanzaoren Tang on mitochondria-mediated neuronal apoptosis. Method:Fifty male Wistar rats were randomly divided into the control group, model group, estazolam group (0.18 mg·kg<sup>-1</sup>·d<sup>-1</sup>), and low- (6.48 g·kg<sup>-1</sup>·d<sup>-1</sup>) and high-dose (12.96 g·kg<sup>-1</sup>·d<sup>-1</sup>) Suanzaoren Tang groups. Rats in all groups except for the control group received subcutaneous injection of <italic>D</italic>-galactose and then were deprived of sleep using the multiple platform method after the last administration. Following successful modeling, the rats in each group were treated with intragastric administration of the corresponding drugs for seven consecutive days. The morphology of mitochondria in hypothalamus was observed under a transmission electron microscope. The activities of Na<sup>+</sup>-K<sup>+</sup>-ATPase and Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase in hypothalamus were detected by spectrophotometry. Western blotting was conducted to determine the protein expression levels of cytochrome C (Cyt C), B cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax) and cysteinyl aspartate-specific protease-3 (Caspase-3) in hypothalamus. Result:In the control group, there was no obvious pathological change in mitochondria, which were moderate in size and oval or spindle in shape, with the cristae arranged orderly. Compared with the control group, the model group exhibited abnormal mitochondrial morphology, manifested as obvious swelling, vacuolation, myelin figures, and cristae rupture and reduction. The comparison with the model group revealed that both the estazolam group and high-dose Suanzaoren Tang group alleviated the mitochondrial damage and reduced the vacuolation and swelling. Only some cristae rupture was present. The improvements were more obvious in the high-dose Suanzaoren Tang group. Compared with the control group, the activities of Na<sup>+</sup>-K<sup>+</sup>-ATPase and Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase and the Bcl-2 protein expression in the model group were significantly decreased (<italic>P<</italic>0.01), whereas the protein expression levels of Cyt C, Bax, and Caspase-3 were significantly increased (<italic>P</italic><0.01). Compared with the model group, the activities of Na<sup>+</sup>-K<sup>+</sup>-ATPase and Ca<sup>2+</sup>-Mg<sup>2+</sup>-ATPase and the Bcl-2 protein expression in the estazolam group and the high-dose Suanzaoren Tang group were significantly elevated (<italic>P<</italic>0.01), while the protein expression levels of Cyt C, Bax, and Caspase-3 were significantly lowered (<italic>P<</italic>0.05, <italic>P<</italic>0.01). The activity of Na<sup>+</sup>-K<sup>+</sup>-ATPase and the Bcl-2 protein expression in the low-dose Suanzaoren Tang group were increased significantly (<italic>P<</italic>0.01), but the Bax protein expression was down-regulated (<italic>P<</italic>0.05). Conclusion:Suanzaoren Tang is able to improve the mitochondrial function of hypothalamic nerve cells and inhibit their apoptosis.

14.
Article in Chinese | WPRIM | ID: wpr-906141

ABSTRACT

Objective:To study the mechanism of Suanzaoren Tang in regulating the energy metabolism of myocardial mitochondria in aged rats with chronic rapid eye movement (REM) sleep deprivation through the sirtuin 3 (SIRT3)/superoxide dismutase2 (SOD2) signaling pathway. Method:Fifty male Wistar rats were randomly divided into the control group, model group, estazolam group (0.18 mg·kg<sup>-1</sup>·d<sup>-1</sup>), and low- (6.48 g·kg<sup>-1</sup>·d<sup>-1</sup>) and high-dose (12.96 g·kg<sup>-1</sup>·d<sup>-1</sup>) Suanzaoren Tang groups. Rats in all groups except for the control group received subcutaneous injection of <italic>D</italic>-galactose and then were deprived of sleep using the multiple platform method after the last administration. Following successful modeling, the rats in each group were treated with intragastric administration of the corresponding drugs for seven consecutive days. The morphology of myocardial mitochondria was observed under a transmission electron microscope. The content of adenosine triphosphate (ATP) in rat hypothalamus was detected by colorimetry, while the malondialdehyde (MDA) content and the SOD activity in myocardium were measured by spectrophotometry. Real-time polymerase chain reaction (Real-time PCR) and Western blot were conducted to determine the mRNA and protein expression levels of SIRT3 and SOD2 in rat myocardium. The localization of SIRT3 was detected by immunofluorescence staining. Result:Compared with the control group, the model group exhibited a disordered arrangement of myocardial filaments, accompanied by filament rupture and dissolution, obviously swollen mitochondria arranged in disorder, and blurring and even rupture of most mitochondrial cristae. Besides, the content of ATP and SOD activity in the myocardium decreased significantly (<italic>P<</italic>0.01), whereas that of MDA increased significantly (<italic>P<</italic>0.01). The mRNA and protein expression levels of SIRT3 and SOD2 were down-regulated significantly (<italic>P<</italic>0.01), and the average fluorescence intensity of SIRT3 protein declined significantly (<italic>P<</italic>0.01). The comparison with the model group revealed that high-dose Suanzaoren Tang enabled the myocardial filaments to be neatly arranged, relieved the mitochondrial damage and swelling, only manifested as partial mitochondrial cristae rupture, significantly increased ATP content, SOD activity, as well as SIRT3 and SOD2 mRNA and protein expression levels (<italic>P<</italic>0.01), reduced the content of MDA (<italic>P<</italic>0.01), and enhanced the average fluorescence intensity of SIRT3 protein (<italic>P<</italic>0.05). The myocardial mitochondrial injury in the estazolam group was also alleviated. The activity of SOD and the SIRT3 and SOD2 mRNA and protein expression levels in the myocardium were significantly elevated (<italic>P<</italic>0.01), while the activity of MDA was significantly lowered (<italic>P<</italic>0.01). In the low-dose Suanzaoren Tang group, the improvement in myocardial mitochondrial injury was not obvious. However, both the SOD activity and SOD2 protein expression were significantly increased (<italic>P<</italic>0.05). Conclusion:Suanzaoren Tang ameliorates the myocardial mitochondria injury and abnormal energy metabolism induced by chronic REM sleep deprivation in aged rats possibly by up-regulating the SIRT3 and SOD2 expression.

15.
Article in Chinese | WPRIM | ID: wpr-906135

ABSTRACT

Presently, tumor has become an important factor threatening human health, and how to cure tumor effectively is still one of the most important problems in the modern medical field. Mongolian medicine has a long history, and is an important component of traditional Chinese medicine (TCM), with distinctive national characteristics. It has been gradually formed and developed by absorbing Tibetan medicine,Indian medical theory and TCM. It has the advantages of a low toxicity,diverse structures and effect in modulating immune responses,with a important value and application perspectives. This paper focused on literatures from China National Knowledge Infrastructure,WanFang and Pubmed databases in recent years,with Mongolian medicine,anti-tumor and mechanism of action as the key words. The relevant literatures were collected, and the anti-tumor mechanisms of Mongolian medicinal in inhibiting cell proliferation, affecting cell cycle,inducing apoptosis,suppressing tumor invasion and metastasis,controlling angiogenesis and regulating immune status were summarized, in the hope to provide a reference for prevention and treatment of tumors with Mongolian medicine. The survey results showed that the study methods for Mongolian medicine at this stage were mostly simple, with a low overall level and based on in vitro cell level. However,the antitumor mechanism of Mongolian medicine compounds was not deeply studied. The material basis and mechanism of Mongolian medicine shall be further studied by modern medicine and bioscience techniques. Long-term goals and plans shall be established to form their own characteristics and advantages.

16.
Article in Chinese | WPRIM | ID: wpr-906122

ABSTRACT

Objective:To study the effects of different arbuscular mycorrhizal(AM) fungi combinations on the growth and quality of<italic> Paris polyphylla</italic> var. <italic>yunnanensis</italic> seedlings,in order to provide reference for the cultivation of high quality <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>. Method:Through the room temperature pot inoculation test method,nine groups of different AM fungi combinations were inoculated into sterilized soil,and the control group was not inoculated. The effects of different AM fungi combinations on root infection rate,photosynthetic parameters,physiological indexes and chemical components of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> seedlings were observed. Result:The spore density in rhizosphere soil,the infection rate and intensity of AM fungi in roots,and the activities of succinate dehydrogenase and alkaline phosphatase in roots of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> were significantly increased by inoculating the combination of exogenous AM fungi. Compared with the control group,the contents of chlorophyll,malondialdehyde,soluble sugar,soluble protein content and the activities of antioxidant enzymes in leaves of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> were increased in different AM fungi treatment groups. The content of total saponin increased from T1 to T2,decreased rapidly from T2 to T4,and increased at T5. Conclusion:Different mixed AM fungus can form a good symbiotic relationship with the roots of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>,improve the mycorrhizal vitality,enhance the resistance of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> to adverse environment,promote the growth and development of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic> and improve the quality of rhizome. According to the comprehensive inoculation effect,the combination of S5,S8 and S9 AM fungus has the best effect,which provides reference value for the application of mycorrhizal biotechnology of <italic>P. polyphylla</italic> var. <italic>yunnanensis</italic>.

17.
Article in English | WPRIM | ID: wpr-922411

ABSTRACT

OBJECTIVES@#To study the effect of glucose metabolism disorders on the short-term prognosis in neonates with asphyxia.@*METHODS@#A retrospective analysis was performed on the medical data of the neonates with asphyxia who were admitted to 52 hospitals in Hubei Province of China from January to December, 2018 and had blood glucose data within 12 hours after birth. Their blood glucose data at 1, 2, 6, and 12 hours after birth (with an allowable time error of 0.5 hour) were recorded. According to the presence or absence of brain injury and/or death during hospitalization, the neonates were divided into a poor prognosis group with 693 neonates and a good prognosis group with 779 neonates. The two groups were compared in the incidence of glucose metabolism disorders within 12 hours after birth and short-term prognosis.@*RESULTS@#Compared with the good prognosis group, the poor prognosis group had a significantly higher proportion of neonates from secondary hospitals (48.5% vs 42.6%, @*CONCLUSIONS@#Recurrent hyperglycemia in neonates with asphyxia may suggest poor short-term prognosis, and it is necessary to strengthen the early monitoring and management of the nervous system in such neonates.


Subject(s)
Asphyxia , Asphyxia Neonatorum/epidemiology , Humans , Hyperglycemia , Infant, Newborn , Prognosis , Retrospective Studies
18.
Article in Chinese | WPRIM | ID: wpr-888333

ABSTRACT

OBJECTIVE@#To analyze the correction loss after posterior segmental fixation for lumbar spine fractures and explore the related image factors.@*METHODS@#Posterior short-segment fixation was received in 48 patients with L@*RESULTS@#The average follow-up was 12 to 18 (16.13±5.39) months. LKA, AVH and VWA at 1 week postoperative and those at the final follow up, were significantly improved compared with those preoperative (@*CONCLUSION@#The angle of adjacent intervertebral discs and anterior height of injured vertebrae were lost statistically after posterior short-segment pedicle screw treatment for lumbar fractures, and multivariate analysis showed that all of them were correlated with load-sharing score.


Subject(s)
Female , Fracture Fixation, Internal , Humans , Lumbar Vertebrae/surgery , Male , Pedicle Screws , Spinal Fractures/surgery , Thoracic Vertebrae/surgery , Treatment Outcome
19.
Article in Chinese | WPRIM | ID: wpr-887980

ABSTRACT

The effects of water regulation on the biosynthesis of calycosin-7-O-β-D-glucoside in 2-year-old Astragalus membranaceus var. mongholicus were studied,and the mechanism was explained from the aspects of key enzyme gene expression and antioxidant enzyme system. The content of calycosin-7-O-β-D-glucoside was determined by HPLC,and the expression levels of six key enzyme genes( PAL,4 CL,CHS,CHI,IFS,13'H) in the synthesis pathway were analyzed by q RT-PCR. The activities of protective enzymes and contents of osmoregulation substances and malondialdehyde were also determined. In the water deficit group,the maximum concentration of calycosin-7-O-β-D-glucoside was 0. 49 mg·g-1 on the 24 th day of treatment. In the whole water regulation,the water deficit group outweighed the water adequate group in osmoregulation substance and MDA contents. The activities of A. membranaceus var.mongholicus antioxidant enzymes SOD,POD,and CAT increased during the initial period of water regulation,but decreased with time.The expression of PAL,CHS,and 13'H in the water deficit group was at a low level,and the 4 CL had active expression,slightly lower than that in the water adequate group. The expression of CHI and IFS elevated rapidly when water deficit occurred. Correlation analysis showed that the content of calycosin-7-O-β-D-glucoside was positively correlated with CHI expression( P<0. 01) and IFS expression( P<0. 05). Therefore,water regulation can change the accumulation pattern of calycosin-7-O-β-D-glucoside,and water deficit may be an effective way to increase its content. CHI and IFS are the key genes in response to water deficit.


Subject(s)
Astragalus propinquus/genetics , Biosynthetic Pathways , Glucosides , Isoflavones , Water
20.
Acta Pharmaceutica Sinica ; (12): 1434-1438, 2021.
Article in Chinese | WPRIM | ID: wpr-887083

ABSTRACT

Seven compounds were isolated from the alcohol extract of Edgeworthia gardneri by various technologies, including silica gel, Sephadex LH-20 and high performance liquid chromatography, and were identified as edgeworthiaside A (1), 2,4,6-trichlorol-3-methyl-5-methoxy-phenol 1-O-β-D-glucopyranosyl-(1-6)-β-D-glucopyranoside (2), 2,6-dimethoxy-4-(2-propen-1-yl)phenyl 6-O-(6-deoxy-α-L-mannopyranosyl)-β-D-glucopyranoside (3), eugenol rutinoside (4), tiliroside (5), edgeworoside C (6), and salicylic acid (7). Compound 1 is a new chlorophenyl glycoside and 2-4 were isolated for the first time from Edgeworthia gardneri. The in vitro inhibition of α-glucosidase showed that the inhibition rate of compounds 1 and 2 were similar to acarbose.

SELECTION OF CITATIONS
SEARCH DETAIL