Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 20 de 20
Filter
Add filters








Year range
1.
Article in Chinese | WPRIM | ID: wpr-928138

ABSTRACT

Quality evaluation of Chinese medicinal decoction pieces is vital for the development of the downstream industries, and is an important channel for implementing the strategy of "higher quality, higher price, and priority for the high quality" for traditional Chinese medicine. At the moment, the quality of Chinese medicinal decoction pieces is mainly evaluated based on chemical component examination. Considering the weak preliminary research foundation and poor research conditions, traditional experience-based evaluation is undervalued in the quality rating of Chinese medicinal decoction pieces. However, traditional experience is a summary of the quality of Chinese medicinal materials based on clinical experience, which thus can be a potential basis for the quality evaluation of the decoction pieces. It is a challenge in the evaluation of Chinese medicinal decoction pieces to objectify the traditional experience-based evaluation from multiple aspects such as chemistry, effect, and characterization via modern techniques. Therefore, this study developed the "experience-ingredients-activity-electronic sensing" evaluation system for Chinese medicinal decoction pieces on the basis of experience-based assessment, chemical ingredients that can truly reflect the traditional experience, biological effect assessment, and electronic sensory evaluation, which is expected to quantify the traditional experience of quality evaluation of Chinese medicinal decoction pieces via chemistry, biology, and sensory simulation. The evaluation system can serve as a reference for clinical experience-based quality evaluation of Chinese medicinal decoction pieces.


Subject(s)
China , Drugs, Chinese Herbal , Electronics , Medicine, Chinese Traditional , Restraint, Physical
2.
Article in Chinese | WPRIM | ID: wpr-905868

ABSTRACT

Objective:To explore effects of different extracts and monomers of <italic>Lepidium meyenii </italic>(Maca) on the proliferation of mouse splenic lymphocytes and induction of interleukin-2 (IL-2) and tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>) by observing their immunomodulatory effects. Method:An octadecylsilyl (ODS) column was used to enrich the methanol extract of <italic>L. meyenii</italic> in stages to obtain six fractions and three monomers. Different groups of extracts and monomers of <italic>L. meyenii </italic>at different doses were set up. Cell counting Kit-8 (CCK-8) was used to detect the effect on the proliferation of mitogen-free, concanavalin A (Con A)-induced, and lipopolysaccharides (LPS)-induced mouse splenic lymphocytes. Enzyme-linked immunosorbent assay (ELISA) was used to determine the levels of IL-2 and TNF-<italic>α</italic>. Result:<italic>L. meyenii </italic>extracts Fr<sub>3</sub> and Fr<sub>6</sub>, and monomers <italic>N</italic>-benzyl hexadecanamide and 1,2-dihydro-4-carboxaldehyde-3-benzyl-<italic>N</italic>-hydroxypyridine slightly promoted the proliferation of Con A-induced T lymphocytes and LPS-induced B lymphocytes (<italic>P</italic><0.01) as compared with the conditions in the model group. <italic>L. meyenii</italic> extracts and monomers significantly induced the secretion of IL-2 and TNF-<italic>α</italic> by splenic lymphocytes (<italic>P</italic><0.01). Conclusion:<italic>L. meyenii</italic> extracts and monomers can achieve immunological enhancement by promoting the secretion of IL-2 and TNF-<italic>α</italic>, and facilitate the proliferation of splenic lymphocytes. The active components are presumedly macamides and pyridine alkaloids, and the specific mechanism still needs to be further explored.

3.
Psychiatry Investigation ; : 636-644, 2021.
Article in English | WPRIM | ID: wpr-903187

ABSTRACT

Objective@#The current study aims to explore precipitating and social risk factors for internet addiction (IA) in university undergraduate students, and to provide evidence for interventions and the early prevention of IA in different genders. @*Methods@#Four thousand eight hundred and fifty-eight college sophomores completed an online survey on their internet use-related behaviours and social risk factors. @*Results@#We found that more male (8.3%) than female students (5.4%) had moderate and severe IA. The main online activity in the moderate and severe IA groups was online gaming in males and online streaming in females. Roommates engaging in similar internetbased entertainment was a risk factor of IA only for males, while not being in a romantic relationship was a risk factor of IA for females only. Infatuation with the internet before college and adjustment problems for college life were shared risk factors for both genders in the mild and moderate IA groups. @*Conclusion@#IA was a common phenomenon in college students with shared and unique precipitating and social risk factors in males and females. The gender-sensitive risk factors for IA warranted earlier and individualized intervention and prevention strategies for IA in this population.

4.
Psychiatry Investigation ; : 636-644, 2021.
Article in English | WPRIM | ID: wpr-895483

ABSTRACT

Objective@#The current study aims to explore precipitating and social risk factors for internet addiction (IA) in university undergraduate students, and to provide evidence for interventions and the early prevention of IA in different genders. @*Methods@#Four thousand eight hundred and fifty-eight college sophomores completed an online survey on their internet use-related behaviours and social risk factors. @*Results@#We found that more male (8.3%) than female students (5.4%) had moderate and severe IA. The main online activity in the moderate and severe IA groups was online gaming in males and online streaming in females. Roommates engaging in similar internetbased entertainment was a risk factor of IA only for males, while not being in a romantic relationship was a risk factor of IA for females only. Infatuation with the internet before college and adjustment problems for college life were shared risk factors for both genders in the mild and moderate IA groups. @*Conclusion@#IA was a common phenomenon in college students with shared and unique precipitating and social risk factors in males and females. The gender-sensitive risk factors for IA warranted earlier and individualized intervention and prevention strategies for IA in this population.

5.
Journal of Stroke ; : 239-246, 2018.
Article in English | WPRIM | ID: wpr-714416

ABSTRACT

BACKGROUND AND PURPOSE: Epidemiological data of cerebral small vessel disease (CSVD) in the general population of China are lacking. We report on the prevalence of lacunes, white matter hyperintensity (WMH), and cerebral microbleeds (CMBs) in a community-based sample in China and compare the results with those of other studies. METHODS: This was a cross-sectional analysis of the population-based Shunyi Study in China. A total of 1,211 stroke-free participants (mean age, 55.6±9.3 years; 37.4% men) with available 3 Tesla (3T) magnetic resonance images were included in this analysis. Demographic information and risk factor data were assessed. The overall and age-specific prevalence of lacunes, WMH, and CMBs was evaluated. Associations between cardiovascular risk factors and the presence of these lesions were analyzed by multiple logistic regression. RESULTS: Our study showed a prevalence of 14.5% for lacunes, 72.1% for periventricular hyperintensity (PVH), 65.4% for deep white matter hyperintensity (DWMH), and 10.6% for CMBs. When compared with other community-based samples, individuals in the same age group showed a higher burden of lacunes and a relatively lower prevalence of CMBs. Advanced age was independently associated with the prevalence of these CSVD markers, while the presence of hypertension increased the risk of lacunes, PVH/DWMH, and CMBs in deep or infratentorial locations. CONCLUSIONS: A higher burden of lacunes but a relatively lower prevalence of CMBs was observed in this Chinese population. This notable result highlights the challenge of CSVD prevention in China. Chinese have a risk factor profile for CSVD similar to those in other populations.


Subject(s)
Asians , Cerebral Small Vessel Diseases , China , Cross-Sectional Studies , Humans , Hypertension , Logistic Models , Prevalence , Risk Factors , White Matter
6.
Article in Chinese | WPRIM | ID: wpr-232275

ABSTRACT

<p><b>OBJECTIVE</b>To assess the association between nitric oxide synthase 1 (NOS1) gene polymorphisms and schizophrenia.</p><p><b>METHODS</b>Twenty eight tag single nucleotide polymorphisms (SNPs) of NOS1 in 382 schizophrenic patients and 448 healthy individuals sampled from Chinese Han population were analyzed by a Illumina GoldenGate Genotyping Assay.</p><p><b>RESULTS</b>One SNP (rs1520811) was found to be associated with schizophrenia, which however becomes negative after Bonferroni correction (P>0.05). Further analysis has failed to identify any association between particular haplotypes and the disease.</p><p><b>CONCLUSION</b>Our results did not support a significant association between NOS1 gene polymorphisms and schizophrenia.</p>


Subject(s)
Adult , Female , Genetic Predisposition to Disease , Haplotypes , Humans , Male , Nitric Oxide Synthase Type I , Genetics , Polymorphism, Single Nucleotide , Schizophrenia , Genetics , Young Adult
7.
Article in English | WPRIM | ID: wpr-284352

ABSTRACT

<p><b>OBJECTIVE</b>To summarize the value and limitations of magnetic resonance (MR) plaque imaging for intracranial arteries.</p><p><b>METHOD</b>The data of MR plaque imaging of intracranial arteries in 422 patients performed in Peking Union Medical College Hospital from December 2006 to September 2010 were analyzed retrospectively.</p><p><b>RESULTS</b>The success rate for MR plaque imaging of intracranial arteries was 97%. MR plaque imaging clearly displayed the wall structure of the arteries trunk (M1 segment of middle cerebral arteries, M2-3 segment of anterior cerebral arteries, and basilar arteries). Good inter-observer (k=0.91, 95% CI=0.80-1.03) and intra-observer reproducibility (k=0.96,95% CI=0.88-1.03) was observed in the evaluation of middle cerebral arteries' atherosclerosis. The wall features had significant difference between symptomatic and asymptomatic intracranial arteries'stenosis; compared with asymptomatic middle cerebral artery stenosis(35 cases), symptomatic middle cerebral artery stenosis(26 cases) had a significantly larger wall area (P=0.000), greater remodeling ratio (P=0.000), higher prevalence of expansive remodeling (outward expansion of the vessel wall) (P=0.003), and lower prevalence of constrictive remodeling (P=0.008). Arteritis of middle cerebral artery (3 cases) showed circular wall-thickening with apparent gadolinium-diethylene triamine pentaacetic acid-biotin enhancement.</p><p><b>CONCLUSION</b>The MR plaque imaging is helpful in the evaluation of intracranial arteries stenosis, although it still has the limitations such as short scan range and low resolution.</p>


Subject(s)
Adolescent , Adult , Aged , Aged, 80 and over , Child , Female , Humans , Intracranial Arteriosclerosis , Diagnosis , Magnetic Resonance Angiography , Methods , Male , Middle Aged , Plaque, Atherosclerotic , Diagnosis , Retrospective Studies , Young Adult
8.
Article in Chinese | WPRIM | ID: wpr-295499

ABSTRACT

<p><b>OBJECTIVE</b>To assess the association between gene polymorphisms and memory function through a genome-wide association study (GWAS) of schizophrenia and control group. Memory cognition was used as a quantitative trait (QT).</p><p><b>METHODS</b>Ninty-eight subjects with chronic schizophrenia and 60 matched controls were genotyped with HumanHap660 Bead Array. The results were correlated with quantitative traits including memory and memory delay.</p><p><b>RESULTS</b>Five candidate genes, including RASGRF2 (rs401758, P = 8.03×10(-5)), PLCG2 (rs7185362, P= 4.54×10(-5)), LMO1 (rs484161, P=9.80×10(-7), CSMD1 (rs2469383, P= 2.77×10(-6)) and PRKG1 (rs7898516, P=6.94×10(-5)) were associated with memory cognition deficits.</p><p><b>CONCLUSION</b>Using memory cognition as a quantitative trait, this Genome- wide association study has identified 5 susceptibility loci. With their association with nervous system development, neuronal growth, axon guidance and plasticity, brain development, above loci may play a role in the development of memory dysfunction in schizophrenia.</p>


Subject(s)
Adult , Female , Genetic Predisposition to Disease , Genome-Wide Association Study , Methods , Humans , Male , Memory , Physiology , Polymorphism, Single Nucleotide , Quantitative Trait Loci , Schizophrenia , Genetics , Young Adult
9.
Article in Chinese | WPRIM | ID: wpr-287391

ABSTRACT

<p><b>OBJECTIVE</b>To explore the role of genetic factors in the brain structural variation by using magnetic resonance imaging scan in schizophrenic patients and their unaffected siblings, and to provide experimental evidence for identifying endophenotype of schizophrenia.</p><p><b>METHODS</b>The optimized voxel-based morphometry (OVBM) was used to process the brain magnetic resonance images in 15 first episode drug-naive schizophrenic patients, 19 unaffected siblings of the patients and 38 normal control subjects. The data were analyzed by using general linear model.</p><p><b>RESULTS</b>Compared to the normal control subjects, significant decreases of gray matter was observed in first episode drug-naive schizophrenia in bilateral temporal lobe, bilateral occipital lobe, left insula, left frontal lobe superior frontal gyrus and right lentiform nucleus medial globus pallidus. Significant increases of gray matter in bilateral parietal lobe, bilateral limbic lobe cingulate gyrus in patients group while compared to controls were also found. In unaffected siblings, significant decreases of gray matter was observed in the right temporal lobe, bilateral occipital lobe, left insula, and left frontal lobe precentral gyrus, and significant increases of gray matter were found in left parietal lobe and bilateral cerebellum posterior lobe. Increased gray matter in left parietal lobe precuneus was found in first episode drug-naive schizophrenia when compared with their unaffected siblings.</p><p><b>CONCLUSION</b>There were similar brain structure abnormalities between the first episode drug-naive schizophrenia and their unaffected siblings. Genetic factor may play important role in brain structural abnormality in schizophrenia, which suggested that the brain structural change might be a genetic endophenotype of schizophrenia.</p>


Subject(s)
Adult , Brain , Congenital Abnormalities , Diagnostic Imaging , Case-Control Studies , Humans , Magnetic Resonance Imaging , Male , Radiography , Schizophrenia , Diagnostic Imaging , Genetics , Pathology
10.
Article in Chinese | WPRIM | ID: wpr-259035

ABSTRACT

Atherosclerosis plaque is the underline cause of ischemic stroke and acute coronary syndrome. In this article, the applications of molecular imaging in vulnerable plaque are reviewed.


Subject(s)
Atherosclerosis , Diagnosis , Pathology , Humans , Molecular Imaging , Methods
11.
Chinese Medical Journal ; (24): 2124-2128, 2008.
Article in English | WPRIM | ID: wpr-350789

ABSTRACT

<p><b>BACKGROUND</b>Retinol binding protein 4 (RBP4), as an adipocyte secreted cytokine, was recently found to be inversely correlated with expression of glucose transporter 4 (GLUT4) in insulin resistance (IR) state and to have an intimate relationship with IR and type 2 diabetes mellitus (T2DM). The present study aimed to evaluate the anti-diabetic efficacy of cinnamaldehyde (Cin), berberine (Ber), and metformin (Met) as well as their impacts on the RBP4-GLUT4 system.</p><p><b>METHODS</b>Rat models of T2DM were established by combination of intraperitoneal injection of low-dose streptozotocin and high fat diet induction. Rats were divided into five groups: the control group, the diabetes group, the diabetes + Ber group, the diabetes + Cin group, and the diabetes + Met group. Western blotting was used to detect the serum or tissue RBP4 and GLUT4 protein levels.</p><p><b>RESULTS</b>After treatment for four weeks, both Cin and Ber displayed significant hypolipidemic, hypoglycemic, and insulin sensitizing functions (P < 0.01) compared with the control group. Their effects on lowering fasting plasma glucose (FPG), low density lipoprotein-cholesterol (LDL-C) and homeostasis model assessment of insulin resistance (HOMA-IR) seem even better than that of Met. Cin and Ber markedly lowered serum RBP4 levels and up-regulated the expression of tissue GLUT4 protein, and Cin seemed more notable in affecting these two proteins.</p><p><b>CONCLUSIONS</b>Both Cin and Ber display an exciting anti-diabetic efficacy in this study and may be of great value for the treatment of type 2 diabetes. Their mechanisms involve the RBP4-GLUT4 system, during which the serum RBP4 levels are lowered and the expression of tissue GLUT4 protein is up-regulated.</p>


Subject(s)
Acrolein , Therapeutic Uses , Animals , Berberine , Therapeutic Uses , Blotting, Western , Body Weight , Diabetes Mellitus, Type 2 , Drug Therapy , Metabolism , Glucose Transporter Type 4 , Blood , Hypoglycemic Agents , Therapeutic Uses , Insulin Resistance , Lipids , Blood , Male , Rats , Rats, Wistar , Retinol-Binding Proteins, Plasma
12.
Article in Chinese | WPRIM | ID: wpr-230018

ABSTRACT

<p><b>OBJECTIVE</b>To describe the magnetic resonance imaging (MRI) manifestations of intraspinal enterogenous cysts, and to provide reliable information for further clinical management.</p><p><b>METHODS</b>The MRI findings and related clinical data of 6 patients with surgically and pathologically confirmed intraspinal enterogenous cysts were retrospectively studied.</p><p><b>RESULTS</b>Among the 6 patients, most enterogenous cysts were oval or fusiform in shape with well-defined margins. Five cysts were located in the subdural extramedullary space and one cyst was located intramedullary. The signals were hypo-or iso-intensity on T1W images and hyperintensity on T2W images. On the postcontrast images, five showed no enhancement, and one was enhanced peripherally.</p><p><b>CONCLUSION</b>The MRI findings of intraspinal enterogenous cysts are specific and the correct preoperative diagnosis may be obtained by integrating MRI findings with clinical data.</p>


Subject(s)
Adult , Central Nervous System Cysts , Diagnosis , Female , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Retrospective Studies , Spinal Cord Diseases , Diagnosis
13.
Article in Chinese | WPRIM | ID: wpr-281275

ABSTRACT

<p><b>OBJECTIVE</b>To study the clinical value of multi-slice spiral CT (MSCT) perfusion technique in evaluation of cerebral hemodynamics in patients with severe carotid stenooclusive lesions.</p><p><b>METHODS</b>Twenty-three patients with severe carotid stenosis were enrolled for evaluation of cerebral hemodynamics, including 12 patients with carotid occlusion and 11 with severe carotid stenosis (> 70%). Among them, 16 patients had multiple arterial diseases, including contralateral carotid stenosis, vertebral arteries stenosis, and cerebral arteries stenosis or occlusion. All patients received CT perfusion examination. Ten normal persons received CT perfusion were regarded as the control group.</p><p><b>RESULTS</b>Cerebral perfusion was bilaterally symmetrical in 10 normal persons. While in the patients group, cerebral hemodynamic impairments ipsilateral to the stenosis side were found by CT perfusion in 17 patients, including an delay of time to peak (TTP) (17 patients), increase of cerebral blood volume (CBV) (7 patients), and decrease of cerebral blood flow (CBF) (4 patients). No significant difference was found between occlusion group (n = 8) and stenosis group (n = 9) (P = 0.640), but incidences of hemodynamic impairment were significantly higher in multiple arterial diseases group (n = 14) than single arterial disease group (n = 3) (P = 0.045).</p><p><b>CONCLUSION</b>CT perfusion technique can sensitively reveal the cerebral hemodynamic impairments in patients with severe carotid stenooclusive diseases, and therefore valuable for clinical application.</p>


Subject(s)
Adult , Aged , Brain , Diagnostic Imaging , Carotid Stenosis , Diagnostic Imaging , Cerebrovascular Circulation , Female , Humans , Male , Middle Aged , Perfusion , Sensitivity and Specificity , Tomography, Spiral Computed , Methods
14.
Article in Chinese | WPRIM | ID: wpr-281274

ABSTRACT

<p><b>OBJECTIVE</b>To explore changes in the carotid arteries in Tarkayasu arteritis (TA) with computed tomographic angiography (CTA).</p><p><b>METHODS</b>The common carotid artery intima-medial thickness (CCA-IMT) in 20 common carotid arteries (CCAs) in 10 controls and 40 CCAs in 20 patients with TA were analyzed and compared.</p><p><b>RESULTS</b>Contrast-enhanced CT scanning combined with the application of 3D reconstruction clearly showed the carotid vascular luminal abnormalities, such as stenosis, occlusion, dilation, and aneurysm formation. CCA-IMT increased in all the patients with TA affecting carotid arteries. CCA-IMT was significantly larger in the patients than in controls [(0.36 +/- 0.13) cm vs. (0.03 +/- 0.02) cm, P < 0.05] , and was significantly larger in patients with active diseases than in those without active diseases [(0.44 +/- 0.09) cm vs. (0.24 +/- 0.10) cm, P < 0.05].</p><p><b>CONCLUSION</b>CCA-IMT increases in TA affecting carotid arteries, especially in active diseases.</p>


Subject(s)
Adolescent , Adult , Carotid Arteries , Diagnostic Imaging , Pathology , Female , Humans , Male , Middle Aged , Sensitivity and Specificity , Takayasu Arteritis , Diagnostic Imaging , Tomography, Spiral Computed , Methods
15.
Article in Chinese | WPRIM | ID: wpr-281264

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the difference of the 3D image quality between the low-dose and the standard-dose chest CT.</p><p><b>METHODS</b>Eleven patients were randomly scanned with the standard-dose CT and 9 with the low-dose CT. Quality of the images from both groups was evaluated by different postprocessing techniques.</p><p><b>RESULTS</b>The effective tube current, total tube current, and CT dose index volume were (114.55 +/- 26.20) mAs, (1578.27 +/- 353.09) mAs, and (7.76 +/- 1.77) mGy, respectively, in the standard-dose scanning group, and were 20 mAs, (566.11 +/- 46.23) mAs, and (1.46 +/- 0.07) mGy, respectively, in the low-dose scanning group. All these indicators had significant differences between these two groups (P < 0.001). The image noises were (17.67 +/- 3.24) HU in standard-dose group and (31.61 +/- 9.42) HU in low-dose group (P < 0.001). As shown by different postprocessing techniques, the median score of the standard-dose group and the low-dose group were 4.52 and 4.78, respectively, which was not significantly different (P = 0.92).</p><p><b>CONCLUSION</b>The low-dose CT scanned with 20 mAs effective tube current can remarkably reduce the radiation dose, and provide satisfactory postprocessing images for diagnosis.</p>


Subject(s)
Adult , Aged , Aged, 80 and over , Female , Humans , Imaging, Three-Dimensional , Male , Middle Aged , Radiation Dosage , Radiography, Thoracic , Methods , Tomography, X-Ray Computed , Methods
16.
Article in Chinese | WPRIM | ID: wpr-281253

ABSTRACT

<p><b>OBJECTIVE</b>To determine the diagnostic accuracy of 16-slice spiral CT in assessing the lower extremity arterial occlusive diseases.</p><p><b>METHODS</b>Thirty patients with suspected peripheral arterial occlusive diseases underwent 16-slice spiral CT angiography (CTA) of lower extremity arteries. All these patients also underwent digital subtraction angiography (DSA) two weeks before or after CTA. CT angiograms were produced using maximal intensity projection, volume rendering technique, and curved planar reconstruction. Using DSA as the standard reference, the diagnostic accuracy of CTA was determined.</p><p><b>RESULTS</b>When detecting segments with > or = 50% stenosis, the sensitivity, specificity, accuracy, positive predictive value (PPV), and negative predictive value (NPV) of CTA were 94.3% (115/122), 98.4% (548/557), 97.6% (663/679), 92.7% (115/124), and 98.7% (548/555), respectively.</p><p><b>CONCLUSION</b>16-slice spiral CT has high sensitivity, specificity, and accuracy in detecting lower extremity arterial occlusive diseases and may partially substitute DSA examinations.</p>


Subject(s)
Aged , Arterial Occlusive Diseases , Diagnostic Imaging , Female , Humans , Lower Extremity , Male , Middle Aged , Sensitivity and Specificity , Tomography, Spiral Computed
17.
Article in English | WPRIM | ID: wpr-243583

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate metabolic abnormalities in patients with mesial temporal lobe epilepsy (MTLE) with proton magnetic resonance spectroscopy (MRS) using a 3. 0T MR scanner.</p><p><b>METHODS</b>Sixty-three patients (32 women and 31 men) with diagnosed MTLE underwent diagnostic MR imaging (MRI) and proton MRS using a 3. 0T MR scanner. The clinical history and interictal epileptiform discharges (IEDs) were recorded. Sixteen healthy volunteers served as control. The results of proton MRS were compared with the findings of electroencephalogram and structural MRI findings.</p><p><b>RESULTS</b>Twenty-seven of the 63 patients with MTLE showed unilateral hippocampal sclerosis, and 9 showed bilateral hippocampal sclerosis. The concentration ratio of N-acytelaspartate (NAA)/[creatine (Cr) + choline (Cho)] in the hippocampal region of MTLE patients (0.64 +/- 0.07) was significantly lower than control (0.80 +/- 0.05, P = 0.023). In the patients with unilateral hippocampal sclerosis, NAA/(Cr + Cho) in the hippocampal region ipsilateral to the sclerotic hippocampus (0.56 +/- 0.06) was significantly lower than the ratio in the contralateral hippocampal region (0.69 +/- 0.07, P < 0.001). There was significant difference in hippocampal NAA/(Cr + Cho) between the refractory patients and the non-refractory patients (0.64 +/- 0.05 vs. 0.71 +/- 0.07, P = 0.04). Forty-five patients were lateralized by IEDs, while 49 patients were lateralized by metabolite ratio. And lateralization determined by proton MRS and IEDs was concordant in 33 patients.</p><p><b>CONCLUSIONS</b>MRS as a noninvasive tool adds helpful metabolite information to routine MRI in evaluation of MTLE. The method is well established and should be a routine clinical application in the investigation of epilepsy.</p>


Subject(s)
Adult , Aspartic Acid , Metabolism , Choline , Metabolism , Creatine , Metabolism , Electroencephalography , Epilepsy, Temporal Lobe , Metabolism , Female , Hippocampus , Metabolism , Humans , Magnetic Resonance Imaging , Magnetic Resonance Spectroscopy , Methods , Male , Protons
18.
Article in English | WPRIM | ID: wpr-243582

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of long-term low dose hormone replacement therapy (HRT) on postmenopausal women in hormone level, cognition score, hippocampus volume, and magnetic resonance spectroscopy (MRS) parameters.</p><p><b>METHODS</b>A total of 182 postmenopausal women aged 50-87 years were chosen at Peking Union Medical College Hospital and assigned to HRT group and control group. The volunteers of HRT group had taken low dose hormone [estradiol (E2) 0.5-1.0 mg and progesterone 0.5-2.0 mg, once a day] for 4-33 years. The concentrations of E2, progesterone, and testosterone were measured using enzyme-linked immunosorbent assay (ELISA). The gene types of apolipoprotein E (ApoE) were measured by polymerase chain reaction, and the subjects with susceptible genes (ApoE epsilon3/epsilon4) of Alzheimer's disease (AD) were screened. Their hippocampus volumes and MRS parameters were obtained through magnetic resonance imaging (MRI), and results in two groups were analyzed by statistical method.</p><p><b>RESULTS</b>Compared with control group, the concentrations of E2 at each age stage in HRT group were significantly higher (P < 0.05) except the 80-89 years old subgroup; yet, there were no statistical differences in the concentrations of progesterone and testosterone between the two groups. There was no obvious difference in ApoE subtypes distribution between the two groups. The results of hippocampus MRI for the subjects with susceptible genes ApoE epsilon3/epsilon4 (HRT group 14 cases, control group 11 cases) showed that the ratio of bilateral hippocampus volume to whole brain volume in HRT group (0.406 +/- 0.028) was significantly higher than control group (0.369 +/- 0.031, P < 0.05). The results of 1H MRS for the subjects with susceptible genes ApoE epsilon3/epsilon4 (HRT group 12 cases, control group 11 cases) showed that the N-acetylaspartate/total creatine at the area of hippocampus in HRT group (1.54 +/- 0.08) were significantly higher than control group (1.45 +/- 0.13, P < 0.05).</p><p><b>CONCLUSIONS</b>For postmenopausal women, long-term low dose HRT can maintain the physiological concentration of E2 in plasma. Furthermore, the hippocampus MRI performed on those with ApoE epsilon3/epsilon4 genes shows that long-term low dose HRT can prevent hippocampus atrophy, which is beneficial to maintain the brain function and prevent AD.</p>


Subject(s)
Aged , Aged, 80 and over , Alzheimer Disease , Apolipoprotein E3 , Genetics , Aspartic Acid , Metabolism , Creatine , Metabolism , Dose-Response Relationship, Drug , Estradiol , Metabolism , Estrogen Replacement Therapy , Female , Hippocampus , Metabolism , Humans , Magnetic Resonance Spectroscopy , Methods , Middle Aged , Postmenopause , Metabolism , Progesterone , Metabolism , Testosterone , Metabolism
19.
Article in Chinese | WPRIM | ID: wpr-327026

ABSTRACT

<p><b>OBJECTIVE</b>To assess the imaging findings in mucinous cystic neoplasms (MCNs) of the pancreas.</p><p><b>METHODS</b>The imaging manifestations of 18 cases with pathologically proven MCNs were analyzed retrospectively.</p><p><b>RESULTS</b>The cystic features of MCNs could be demonstrated by ultrasonography (US), computed tomography (CT), or magnetic resonance imaging (MRI). Compared with US, CT and MRI are appropriate for localizing diagnosis (100%). One half of the cases have classic imaging findings of the MCNs, where as the diagnosis of remaining cases could be supported by clinical findings.</p><p><b>CONCLUSIONS</b>Imaging technique plays an important role in the diagnosis of MCNs.</p>


Subject(s)
Adult , Aged , Cystadenoma, Mucinous , Diagnosis , Diagnostic Imaging , Female , Humans , Magnetic Resonance Imaging , Male , Middle Aged , Pancreas , Pancreatic Neoplasms , Diagnosis , Diagnostic Imaging , Retrospective Studies , Tomography, X-Ray Computed
20.
Article in Chinese | WPRIM | ID: wpr-679673

ABSTRACT

Objective To investigate hepatocyte growth factor(HGF)gene expression and biological effect after gene transfection into penumbra tissue in rat cerebral ischemic model.Methods Human HGF cDNA was ligated to pIRES2-EGFP vector.The recombinant plasmid was transfected into the penumbra tissue with liposome.Brains of treated and control animals were analyzed 7 days later.Expression of HGF protein was determined by fluorescence microscopy and immunohistochemistry.Vessel numbers were quantified.Changes of cerebral blood flow(CBF)was detected by CT perfusion.Results Enzymatic digestion and electrophoresis confirmed that HGF fragment had been correctly cloned into BamH I and Sal I sites of pIRES2-EGFP.After HGF gene transfection,expression of HGF in transfected neurocytes was observed with fluorescence microscopy and immunohistochemistry.The number of vessels was significantly increased in penumbra tissue transfected with HGF vector as compared with control vector(46.71?7.11, 20.43?3.21,18.00?3.27,respective,F = 74.447;P

SELECTION OF CITATIONS
SEARCH DETAIL