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1.
Article in English | WPRIM | ID: wpr-922108

ABSTRACT

OBJECTIVE@#To observe the clinical effect of penetrating moxibustion on migraine without aura (MO) patients.@*METHODS@#Totally 60 MO patients from the Acupuncture Clinic of the Third Affiliated Hospital of Henan University of Chinese Medicine were collected from November 2015 to February 2017. All patients were assigned to a treatment group and a control group using a random number table, 30 cases in each group. The treatment group was treated with penetrating moxibustion, and the control group was treated with mild moxibustion, thrice a week for 4 consecutive weeks. The total effective rate, Visual Analogue Scale (VAS) scores, headache intensity, and Migraine Specific Quality of Life Questionnaire (MSQ) scores of patients after treatment were compared between the two groups. The moxibustion sensation and reaction after moxibustion were observed, and the adverse reactions were evaluated. All patients were followed up at 4 and 16 weeks after treatment.@*RESULTS@#The total effective rate of the treatment group was significantly higher than that of the control group (93.33% vs. 80.00%, P0.05).@*CONCLUSIONS@#Penetrating moxibustion can significantly relieve pain and improve quality of life of MO patients. After penetrating moxibustion, flushing and sweating of patients were obvious, and the curative effect was superior to the mild moxibustion.


Subject(s)
Acupuncture Points , Humans , Migraine without Aura/therapy , Moxibustion/adverse effects , Quality of Life , Treatment Outcome
2.
Article in Chinese | WPRIM | ID: wpr-873160

ABSTRACT

Objective:To observe the clinical efficacy of Tongxie Yaofang on irritable bowel syndrome-D (IBS-D) of liver-stagnation and spleen-deficiency type, in order to explore its mechanism in regulating brain-intestine interaction by changing the intestinal flora before and after treatment. Method:Totally 116 patients with IBS-D with liver stagnation and spleen deficiency who were diagnosed from July 2016 to December 2018 were randomly divided into observation group and control group, with 58 patients in each group. Observation group was treated with Tongxie Yaofang orally. Control group was treated with pivalvonium orally. Both groups were treated for 4 weeks. Scores of traditional Chinese medicine pattern curative effect scoring system (TCM-PES), IBS quality of life questionnaire (IBS-QOL), self-rating anxiety scale (SAS) and self-rating depression scale (SDS) of two groups were compared before and after treatment. Calcitonin gene-related peptide (CGRP) and vasoactive intestinal peptide (VIP) were detected before and after treatment by enzyme-linked immunosorbent assay (ELISA). Real-time fluorescent quantitative polymerase chain reaction(Real-time PCR) was used to detect changes in Escherichia coli, Bifidobacteria, Lactobacillus acidophilus and Streptococcus faecalis and other intestinal flora before and after treatment. Result:TCM-PES and IBS-QOL scores of two groups were improved after treatment. TCM-PES and IBS-QOL scores of observation group were higher than those of control group (P<0.05). TCM syndromes of observation group were significantly higher than those of control group (P<0.05). SAS and SDS scores were significantly lower after treatment. SAS and SDS scores of observation group were lower than those of control group after treatment (P<0.05). Plasma CGRP and VIP decreased after treatment. Plasma CGRP and VIP in observation group were significantly lower than those in control group (P<0.05). There was no significant change in E. coli after treatment in two groups. After treatment, L. acidophilus, Bifidobacterium, and S. faecalis increased (P<0.05). In control group, intestinal L. acidophilus increased after treatment (P<0.05). The differences of intestinal L. acidophilus, Bifidobacterium, S. faecalis in two groups were statistically significant (P<0.05). Conclusion:TCM can alleviate clinical symptoms, such as abdominal pain and diarrhea in patients with IBS-D, improve patients' bad mood and improve their quality of life. This may be related to improvement of intestinal flora imbalance, regulation of brain intestinal peptide secretion and reduction of visceral hypersensitivity.

3.
Journal of Medical Postgraduates ; (12): 280-284, 2020.
Article in Chinese | WPRIM | ID: wpr-818419

ABSTRACT

ObjectiveCardiovascular disease (CVD) is the main cause of morbidity and mortality in patients with hemodialysis (HD) end-stage renal disease (ESRD). This paper analyzes and discusses the relationship between neutrophils-to-lymphocytes ratio (NLR) and heart valve calcification (CVC) in maintenance hemodialysis (MHD) patients to provide theoretical basis for the prevention and treatment of CVC.MethodsThe demographic data, relevant clinical indicators and laboratory examination results of 135 patients with MHD in the Second Hospital of Anhui Medical University were retrospectively analyzed to calculate the NLR value. Echocardiography was used to detect the incidence of CVC in the patients, and they were divided into calcification group and non-calcification group. The correlation between NLR value and CVC in MHD patients was analyzed, and the independent risk factors of CVC were discussed by using Logistic regression.ResultsAmong the 135 MHD patients, CVC was found in 59 cases (43.7%). Compared with the non-calcification group, patients in the calcification group showed significant increases in age, dialysis age, high-sensitivity c-reactive protein (HsCRP), ALP and NLR, with statistically significant differences (P5.02 (OR=17.709, P=0.046) were independent risk factors for heart valve calcification in MHD patients.ConclusionThe incidence of heart valve calcification is high in MHD patients, and NLR is an independent risk factor for it.

4.
Article in Chinese | WPRIM | ID: wpr-773159

ABSTRACT

The chemical constituents from the fruiting bodies of Tremella sanguinea were separated and purified by column chromatography on silica gel,ODS,Sephadex LH-20,and RP-HPLC. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic data analysis,as well as comparisons with the data reported in the literature. Sixteen compounds were isolated from the 90% ethanol extract of the fruiting bodies of T. sanguinea,which were identified as( 22 E)-5α,8α-epidioxy-24-methyl-cholesta-6,9( 11),22-trien-3β-ol( 1),( 22 E)-5α,8α-epidioxyergosta-6,22-dien-3β-ol( 2),cerevisterol( 3),ergosta-7-ene-3β,5α,6β-triol( 4),( 22 E)-6β-methoxyergosta-7,22-diene-3β,5α-diol( 5),ergosta-7-en-3β-ol( 6),4-hydroxy-methylincisterol( 7),2-pyrrolidone( 8),nicotinamide( 9),1-( 3-indolyl)-3-dihydroxypropan-1-one( 10),yangambin( 11),linoleic acid( 12),( 9 Z,12 Z,15 Z)-2,3-dihydroxypropyl octadeca-trienoate( 13),( 9 Z,12 Z)-2,3-dihydroxypropyl-octadeca-dienoate( 14),crypticin B( 15)and 3-phenyllactic acid( 16). All compounds were isolated from T. sanguinea for the first time. Except for compounds 6,9 and 12,the remained compounds were isolated from the genus Tremella for the first time.


Subject(s)
Basidiomycota , Chemistry , Fruiting Bodies, Fungal , Chemistry , Molecular Structure
5.
Article in Chinese | WPRIM | ID: wpr-773122

ABSTRACT

The chemical constituents from the stems and leaves of Clausena emarginata were separated and purified by column chromatographies on silica gel,ODS,Sephadex LH-20,and PR-HPLC. The structures of the isolated compounds were identified on the basis of physicochemical properties and spectroscopic analysis,as well as comparisons with the data reported in the literature. Sixteen compounds were isolated from the 90% ethanol extract of the stems and leaves of C. emarginata,which were identified as siamenol( 1),murrastanine A( 2),3-formyl-1,6-dimethoxycarbazole( 3),3-methoxymethylcarbazole( 4),3-methylcarbazole( 5),murrayafoline A( 6),3-formylcarbazole( 7),3-formyl-1-hydroxycarbazole( 8),3-formyl-6-methoxycarbazole( 9),murrayanine( 10),murrayacine( 11),girinimbine( 12),nordentatin( 13),chalepin( 14),8-hydroxy-6-methoxy-3-pentylisocoumarin( 15) and ethyl orsellinate( 16). Compounds 1-4,14-16 were isolated from C. emarginata for the first time. Among them,compounds 1,2,15 and 16 were isolated from the genus Clausena for the first time. All isolated compounds were evaluated for their cytotoxic activities against five human cancer cell lines: HL-60,SMMC-7721,A-549,MCF-7 and SW480 in vitro. Compounds 12 and 14 showed significant inhibitory effects against various human cancer cell lines with IC_(50) values comparable to those of doxorubicin.


Subject(s)
Antineoplastic Agents, Phytogenic , Pharmacology , Cell Line, Tumor , Clausena , Chemistry , Doxorubicin , Humans , Phytochemicals , Pharmacology , Plant Leaves , Chemistry , Plant Stems , Chemistry
6.
Article in Chinese | WPRIM | ID: wpr-838268

ABSTRACT

Objective To explore the relationship between D320N locus polymorphism in proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and coronary heart disease. Methods A total of 3 450 patients with coronary heart diseases (angina pectoris, myocardial infarction, asymptomatic myocardial ischemia, ischemic cardiomyopathy, and sudden death), who were treated in Hebei General Hospital from Jan. 2014 to Jan. 2016, were enrolled as the case group, with 1 100 healthy people in the same period served as controls. Serum lipid levels and PCSK9 concentrations of subjects in the two groups were detected. PCR assay combined with DNA direct sequencing method was applied in genotyping of D320N (A/G) polymorphic locus in PCSK9 gene. Logistic regression analysis was used to analyze the relationship between D320N locus polymorphism in PCSK9 gene and different types of coronary heart diseases. Results Serum concentrations of PCSK9, levels of total cholesterol, triacylglycerol and low density lipoprotein cholesterol, ratio of coronary heart disease family, and ratio of smoking of subjects in the case group were significantly higher than those in the control group (P0.001), while the level of high density lipoprotein cholesterol was significantly lower than that in the control group (P0.001). The frequencies of genotype AA and GA in the case group were 3.6% and 13.9%, respectively, which were significantly higher than those in the control group, respectively (1.1%, 7.0%; χ2=20.502, 39.646; P0.001); the frequency of allele A in the case group was significantly higher than that in the control group (10.5% vs 4.6%, χ2=70.481, P0.001). The frequencies of genotype AA, genotype GA and allele A were the highest in the sudden death group, and were the lowest in the asymptomatic myocardial ischemia group. Logistic regression analysis showed that genotypes AA and GA of D320N (A/G) polymorphic in PCSK9 gene were risk factors of coronary heart disease (both P0.001). Conclusion The D320N (A/G) locus polymorphism in PCSK9 gene was associated with coronary heart disease, and genotype AA may be an independent risk factor of coronary heart disease.

7.
Article in Chinese | WPRIM | ID: wpr-691470

ABSTRACT

Objective: To present a prognostic model for evaluating the outcome of root canal treatment in teeth with pulpitis or apical periodontitis 2 years after treatment. Methods: The implementation of this study was based on a retrospective study on the 2-year outcome of root canal treatment. A cohort of 360 teeth, which received treatment and review, were chosen to build up the total sample size. In the study, 143 teeth with vital pulp and 217 teeth with apical periodontitis were included. About 67% of the samples were selected randomly to derive a training date set for modeling,and the others were used as validating date set for testing. Logistic regression models were used to produce the prognostic models. The dependent variable was defined as absence of periapical lesion or reduction of periapical lesion. The predictability of the models was evaluated by the area under the receiver-operating characteristic (ROC ) curve (AUC). Results: Four predictors were included in model one (absence of apical lesion): preoperative periapical radiolucency,canal curvature,density and apical extent of root fillings. The AUC was 0. 802 (95% CI: 0. 744-0. 859). And the AUC of the testing date was 0. 688. Only the density and apical extent of root fillings were included to present model two (reduction of apical lesion). The AUC of training dates and testing dates were 0. 734 (95% CI: 0. 612-0. 856) and 0. 681,respectively. As predicted by model one,the probability of absence of periapical lesion 2 years after endodontic treatment was 90% in pulpitis teeth with sever root-canal curvature and adequate root canal fillings, but 51% in teeth with apical periodontitis. When using prognostic model two for prediction, in teeth with apical periodontitis, the probability of detecting lesion reduction with adequate or inadequate root fillings was 95% and 39% 2 years after treatment. Conclusion: The pre-operative periapical status, canal curvature and quality of root canal treatment could be used to predict the 2-year outcome of root canal treatment.

8.
Article in Chinese | WPRIM | ID: wpr-690503

ABSTRACT

Six kinds of provenance of Rheum tanguticum collected from Qinghai province as the test materials, which were transplanted under 3 different environments by using complete randomized block design with three replicates. The contents of the chemical components was determined by HPLC. This study aimed at analyzing the effect of genotype, environment and their interactions on the 4 kinds of functional components (phenolic acids, bianthrone, free anthraquinones and combined anthraquinones) in 14 kinds of active components of Rh. tanguticum, in order to provide a theoretical basis for the selection of cultivated Rh. tanguticum in high quality producing area and excellent provenance. The provenance trial showed that the genotype and environment influence on the effect of all kinds of functional components in Rh. tanguticum were significant (<0.05). The content of phenolic acids was mainly influenced by environment, and the other three kinds of functional components were affected by environment and their interactions. The proportion of environment was larger. The cultivation quality of Rh. tanguticum should give priority to environment, then choose a provenance. Sichuan may be beneficial in accumulation of combined anthraquinones in Rh. tanguticum, Gansu may facilitate the binding of free anthraquinone, phenolic acids and bianthrone content. Preliminary inference based on the content and proportion of efficacy components, T4 could be potential special medicinal germplasm that have function of heat-clearing and detoxifying drugs and activate blood circulation to dissipate blood stasis; T3 and T6 could all be potential specialmedicinal germplasms that exist diarrhea attack characters. The results of this study have certain guiding significance for the production of rhubarb precision medicinal materials.

9.
Article in Chinese | WPRIM | ID: wpr-687428

ABSTRACT

Eight kinds of provenance of Rheum palmatum collected from 4 provinces Sichuan, Ningxia, Gansu, Shannxi as test materials, which were transplanted under 3 different environments by using complete randomized block design with three replicates. The contents of the chemical components was determined by HPLC. This study aimed at analyzing the effect of genotype, environment and their interactions on the 4 kinds of functional components (phenolic acids, bianthrone, free anthraquinones and combined anthraquinones) in 14 kinds of active components of R. palmatum, in order to provide a theoretical basis for the selection of cultivated R. palmatum in high quality producing area and excellent provenance. The functional components of R. palmatum were influenced by genotype and environment. The content of phenolic acids was mainly influenced by environment, and the other three kinds of functional components were affected by environment and their interactions. The proportion of environment was larger. The cultivation quality of R. palmatum should give priority to environment, then choose a provenance. Sichuan may be beneficial in accumulation of free anthraquinones in R. palmatum, Gansu may facilitate the binding of combined anthraquinone, phenolic acids and bianthrone content. Preliminary inference based on the content and proportion of efficacy components, P2 could be potential special medicinal germplasm that have function of heat-clearing and detoxifying drugs. P6 could be potential special medicinal germplasm that activate blood circulation to dissipate blood stasis. P7 and P1 could all be potential specialmedicinal germplasms that exist diarrhea attack characters. The results of this study have important guiding significance for the production of rhubarb precision medicinal materials.

10.
Tianjin Medical Journal ; (12): 1162-1166, 2017.
Article in Chinese | WPRIM | ID: wpr-667858

ABSTRACT

Objective To analyze the clinical characteristics of very elderly patients (age ≥80 years) with acute myocardial infarction (AMI) and to provide basis for the treatment and prevention of AMI. Methods The clinical data of 296 patients with ST-segment elevation AMI who admitted in the Second Hospital of Tianjin Medical University during May 2012 to July 2014 were retrospectively analyzed. The patients were divided into very elderly group (≥80 years old, n=54), elderly group (60-79 years old, n=148) and younger group (<60 years old, n=94). The clinical baseline data, infarction area, imaging data, medication in-hospital and adverse cardiovascular events were compared between the three groups. Results The proportion of smoking, diastolic blood pressure, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), erythrocyte count (RBC) and hemoglobin (HGB) at admission were significantly lower in the very elderly group. Results of electrocardiogram (ECG) showed mainly anterior wall infarction in three groups, and there were higher proportions of multiple infarcts in very elderly group and elderly group than those of younger group (31.5%vs. 31.8%vs. 17.0%,χ2=6.994, P < 0.0167). Coronary angiography (CAG) indicated that the target vessels were mostly right coronary arteries ( 61.1%) in very elderly group, while they were mainly left anterior descending (proportions were 50.7% and 43.6% respectively) in elderly group and the younger group. The proportion of high thrombotic load, the proportion of temporary cardiac pacing and no-reflow rate were significantly higher but the proportion of percutaneous coronary intervention (PCI) was lower in the very elderly group than those of younger group. The result of ultrasonic cardiogram (UCG) illustrated that the diameter of the left atrial diameter (LAD) and the left ventricular end-systolic dimension(LVDs) were larger in very elderly group than those of younger group (P<0.0167). The left ventricular ejection fraction (LVEF) was lower in the very elderly group and the elderly group than that of the young group, and the incidence of ventricular aneurysm was higher than that of the young group.During the hospitalization, the percentage of using diuretics, positive inotropic drugs and intraoperative tirofiban were higher in the very elderly group than those of elderly group and younger group. Meanwhile, all-cause mortality, the percentage of new-onset atrial fibrillation (NOAF) and the percentage of atrioventricular block were also higher in the very elderly group compared with those of other two groups (P < 0.0167). Conclusion The very elderly patients with AMI are generally related with complex diseases and more postoperative complications. It is important to perform the blood vessel recanalization early according to the specific conditions, and to assess the risk of postoperative bleeding, thrombosis and other related risks in order to select the appropriate treatment with low mortality and less risk of hospital complications in the very elderly patients with AMI.

11.
Article in Chinese | WPRIM | ID: wpr-659855

ABSTRACT

Objective To study the effect of quercetin on proliferation of hepatocarcinoma cells and the expression of miR-199a. Methods Hepatocellular carcinoma cell line HepG2 cells were transfected with 20 μm/L quercetin. MTT proliferation assay were conducted to compare the proliferation, and Annexin V/PI apoptosis assay were adopted to compare the apoptosis of cells in quercetin group and NC group at 12 h, 24 h, 36 h and 48 h. The expression of miR-199a were determined by RT-qPCR and western blotting assays were used to detect the Western blotting was used to detect the expression of VEGFA, Bax and Caspase3. Results MTT showed that quercetin inhibited the proliferation of HepG2 cells (P<0.01). The apoptosis of hepatocellular carcinoma was markedly increased after treated with quercetin (P<0.01). Moreover, quercetin can up-regulate the expression of miR-199a in HepG2 cells. The quercetin decreased the expression of VEGFA, increased the expression of Bax and Caspase 3 on both mRNA and protein level (P<0.01). Conclusion Quercetin can inhibit the proliferation and promote apoptosis of hepatocarcinoma cells by up-regulating the expression of miR-199a.

12.
Article in Chinese | WPRIM | ID: wpr-657594

ABSTRACT

Objective To study the effect of quercetin on proliferation of hepatocarcinoma cells and the expression of miR-199a. Methods Hepatocellular carcinoma cell line HepG2 cells were transfected with 20 μm/L quercetin. MTT proliferation assay were conducted to compare the proliferation, and Annexin V/PI apoptosis assay were adopted to compare the apoptosis of cells in quercetin group and NC group at 12 h, 24 h, 36 h and 48 h. The expression of miR-199a were determined by RT-qPCR and western blotting assays were used to detect the Western blotting was used to detect the expression of VEGFA, Bax and Caspase3. Results MTT showed that quercetin inhibited the proliferation of HepG2 cells (P<0.01). The apoptosis of hepatocellular carcinoma was markedly increased after treated with quercetin (P<0.01). Moreover, quercetin can up-regulate the expression of miR-199a in HepG2 cells. The quercetin decreased the expression of VEGFA, increased the expression of Bax and Caspase 3 on both mRNA and protein level (P<0.01). Conclusion Quercetin can inhibit the proliferation and promote apoptosis of hepatocarcinoma cells by up-regulating the expression of miR-199a.

13.
Article in Chinese | WPRIM | ID: wpr-279004

ABSTRACT

<p><b>OBJECTIVE</b>To study the differences in the clinical features of purulent meningitis (PM) between small-for-gestational-age (SGA) and appropriate-for-gestational-age (AGA) infants.</p><p><b>METHODS</b>The clinical data of 58 full-term infants with PM were analyzed retrospectively. The infants were classified into a SGA group (13 cases) and an AGA group (45 cases) according to their birth weight and gestational age. Clinical manifestations, laboratory results, and outcomes were compared between the two groups.</p><p><b>RESULTS</b>The incidence of decreased muscle tone in the SGA group was significantly higher than that in the AGA group (P<0.05); the positive rate in the Pandy's test for cerebrospinal fluid in the SGA group was significantly higher than that in the SGA group (P<0.05). Brain imaging examination showed that the incidence of brain injuries in the SGA group was significantly higher than that in the AGA group (P<0.05).</p><p><b>CONCLUSIONS</b>SGA infants with PM display a higher risk of brain injury, suggesting a poorer outcome, compared with AGA infants.</p>


Subject(s)
Brain Injuries , Epidemiology , Female , Humans , Infant, Newborn , Infant, Small for Gestational Age , Magnetic Resonance Imaging , Male , Meningitis, Bacterial , Retrospective Studies , Suppuration
14.
Article in English | WPRIM | ID: wpr-820638

ABSTRACT

OBJECTIVE@#To draw on data about publication patterns and citation indicators of Asian Pacific Journal of Tropical Medicine (APJTM) during 2008 and June 2014 in order to know about the current state of the journal.@*METHODS@#Data used in this study were collected based on publications in APJTM from 2008 to June, 2014. Information including publication issue, type of manuscript, country/region of Corresponding author, funded research paper, and international collaboration were aggregated and analyzed with Excel software. Citation indicators including total cites, average cites of each manuscript, h-index, and impact factors were primarily drawn from Web of Science™ database on June 15, 2014 and changes over the past six and half years were interpreted. The top 10 most cited papers in Web of Science™ database were also analyzed.@*RESULTS@#Number of all submissions has arisen from less than 200 in 2008 to over 1 500 in 2013, manuscript acceptance rate has decreased to be less than 14.00% indicating its improvement in quality over this period of time. Out of the 1 115 publiations, 23.77% were fruits of funded projects or produced by funded co-authors, 87.08% of all publications in APJTM were submited by authors from 10 most contributed countries. During the studied period, each published manuscript in the journal has received an average of 1.05 cites, and at least 10 publications has been cited for more 10 times.@*CONCLUSION@#Detailed analysis shows APJTM has made great progress over the past six and half years, but authors' originating countries are still disproportionate. Efforts should be made to improve its citation indicators.


Subject(s)
Manuscripts as Topic , Periodicals as Topic , Publishing
15.
Article in Chinese | WPRIM | ID: wpr-290780

ABSTRACT

<p><b>OBJECTIVE</b>To evaluate the benefit and safety of fluorouracil implants on colorectal cancer.</p><p><b>METHODS</b>Based on the methods of Cochrane systematic reviews, databases including CBM(1982 to March 2011), CNKI(1911 to March 2011), EMBASE(1966 to March 2011), and Medline(1950 to March 2011) were searched to identify randomized controlled trials assessing the benefit of fluorouracil implants on colorectal cancer. The quality of the included studies was assessed using the Cochrane's tool for assessing bias. RevMan5.0 was used for meta-analysis.</p><p><b>RESULTS</b>Sixteen studies were included(n=1223). The quality of included studies was moderate. Fluorouracil implants could reduce the 2-year mortality(RR=0.33. 95% CI:0.18-0.59), 2-year metastasis rate(RR=0.35, 95% CI: 0.19-0.66), and 2-year recurrence rate(RR=0.48, 95% CI:0.36-0.65). There were no significant differences in complications and adverse effects between fluorouracil implants and the control group.</p><p><b>CONCLUSIONS</b>Current evidence demonstrates that fluorouracil implants may modestly improve the outcome of colorectal cancer patients without increasing its adverse events. However, the results should be interpreted with caution due to the risk of bias of included studies.</p>


Subject(s)
Antineoplastic Agents , Therapeutic Uses , Colorectal Neoplasms , Drug Therapy , Fluorouracil , Therapeutic Uses , Humans , Randomized Controlled Trials as Topic , Treatment Outcome
16.
Chinese Journal of Cardiology ; (12): 488-493, 2011.
Article in Chinese | WPRIM | ID: wpr-272214

ABSTRACT

<p><b>OBJECTIVE</b>To study the differential microRNAs expression between patients with essential hypertension and healthy controls.</p><p><b>METHODS</b>Whole blood from 15 hypertensive patients and 5 controls healthies were separated into plasma at 3000 rpm for 10 minutes. MicroRNAs were harvested using kit, and stored at -80°C. MicroRNAs profiling were performed using Exiqon microRCURY(TM) LNA microRNAs array, and were quantitative RT-PCR for the differential microRNAs expression. In addition, we used a set of plasma samples from 24 hypertensive patients and 22 healthy donors to independently validate the expression of these signature microRNAs.</p><p><b>RESULTS</b>MicroRNAs expression profile was found to be differentially in the essential hypertensive patients compared with the healthy donors. Of 1700 microRNAs detected on the microarray, 46 microRNAs were found to be differentially expressed in the essential hypertensive patient, 27 microRNAs were collected in Sanger microRNAs data-bank, the function of remaining 19 microRNAs were unknown. In the 27 microRNAs, 9 microRNAs were up-regulated in the hypertension patient samples, while 18 known microRNAs were down-regulated. MiR-296-5p (Fold change 0.47, P = 0.013) and miR-133b (Fold change 0.57, P = 0.033) were consistently down-regulated in the patient plasma, whereas let-7e (Fold change 1.62, P = 0.009) and hcmv-miR-UL112 (Fold change 2.72, P = 0.004), one human cytomegalovirus encoded microRNAs, were up-regulated in the patient samples. The microRNAs expression was independently validated using another sample. We showed that MHC class I polypeptide-related chain B (MHC class I polypeptide-related chain B, MICB) and Interferon regulatory factor 1 (Interferon regulatory factor 1, IRF1) were functional targets of hcmv-miR-UL112 by fluorescent reporter assays.</p><p><b>CONCLUSIONS</b>The hypertensive patients have distinct microRNAs expression Profile. Hcmv-miR-UL112 may have important implications toward pathogenesis of essential hypertension.</p>


Subject(s)
Adult , Case-Control Studies , Female , Gene Expression Profiling , Humans , Hypertension , Blood , Genetics , Male , MicroRNAs , Middle Aged , Oligonucleotide Array Sequence Analysis , Transcriptome
17.
Article in Chinese | WPRIM | ID: wpr-326507

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of ecoimmunonutrition supplement on intestinal microecology, epithelial tight junctions, and barrier function in rats with surgical stress.</p><p><b>METHODS</b>Seventy SD rats after surgical trauma were randomly divided into four groups:(1) placebo group,(2)total parenteral nutrition(TPN) group,(3)enteral nutrition(EN) group and (4)ecoimmunonutrition (EEN)group respectively. Rats received isocaloric and isonitrogenous nutrition. Nutrients were administered via the neck vein and the needle jejunostomy for five days. The homogenated tissues of liver, lung, and mesenteric lymph nodes were cultured to determine the bacterial translocation rate. The transmembrane binding proteins(occludin) was measured by immunohistochemistry. The ultrastructure and morphology of intestinal epithelial tight junctions in the intestine were observed by electron microscope. The feces in cecum was cultured for anaerobic bacterial growth and analysed.</p><p><b>RESULTS</b>The amounts of lactobacteria and bifidobacteria in EEN group were significantly higher than those in TPN group(P<0.05). The expression levels of occludin in the intestine was significantly higher in EEN group than that in TPN and EN group. Furthermore, the intestinal epithelial tight junction and microvilli of EEN group were more intact compared with those of TPN group. The bacterial translocation rates of liver, lung and mesenteric lymph nodes were significantly lower in EEN and EN group than those in TPN group(P<0.05).</p><p><b>CONCLUSION</b>Application of ecoimmunonutrition can protect intestinal mucosal barrier in rats with operative stress, increase the expression of occludin, maintain the gut epithelial tight junction, and eliminate gut bacterial translocation.</p>


Subject(s)
Animals , Enteral Nutrition , Gastrointestinal Tract , Microbiology , Intestinal Mucosa , Male , Probiotics , Therapeutic Uses , Rats , Rats, Sprague-Dawley , Surgical Procedures, Operative
18.
Chinese Journal of Pediatrics ; (12): 763-768, 2008.
Article in Chinese | WPRIM | ID: wpr-300679

ABSTRACT

<p><b>OBJECTIVE</b>To assess the effect of blood pressure (BP) changes from childhood to adulthood on hypertension related cardiac and renal function in adulthood.</p><p><b>METHODS</b>The "Beijing children and adolescents BP study cohort" consists of 2505 subjects 6-18 years of age who were enrolled in the baseline BP investigation in 1987. Among them, 412 individuals aged 23-37 years were successfully followed up in 2005. In this study, clinical examinations and questionnaire survey about risk factors of cardiovascular disease (CVD) were carried out.</p><p><b>RESULTS</b>Among 412 adults, the prevalence rate of hypertension was 14.8% (61/412); 83.6% of persons with hypertension were overweight or had obesity. Hypertension related cardiac and renal functional abnormalities were relatively common (72.1%) clinical signs among this adult population. With BP level elevation, cardiac and renal functions damage became more severe. The morbidity of cardiac and renal functional abnormalities in those who were hypertensive in childhood and were not hypertensive in adulthood was similar to those who were not hypertensive at both times. The morbidity of microalbuminuria and abnormalities in electrocardiogram (ECG) were higher in those who were hypertensive at both times than those who had appropriate BP levels in childhood and became hypertensive in adulthood. Adjusted for age, gender, BMI and body surface area (BSA) during adulthood, the left ventricular end-diastolic dimension (LVd), the left ventricular mass (LVM), the intraventricular septal thickness (IVST), the left ventricular posterior wall thickness (LVPWT) in the group of hypertension were higher than that in the group which was not hypertensive, and the ejection fraction (EF) was lower in the group of hypertension in adulthood. The LVd, LVM, IVST, LVPWT and EF in those who were hypertensive in childhood and were not hypertensive in adulthood were similar to those who were not hypertensive at both times. Logistic regression analysis showed that persons who were hypertensive in childhood and remained so during adulthood were at an increased risk of cardiac and renal functional damage (OR = 4.072, 95% CI = 1.472 - 11.266). Those who were hypertensive in childhood and became normotensive in adulthood did not have significant abnormalities in cardiac and renal functions (OR = 1.086, 95% CI = 0.514 - 2.298, chi2 = 0.047, P = 0.828).</p><p><b>CONCLUSION</b>Persons who were hypertensive at both times were at an increased risk of cardiac and renal function damage. Those who were hypertensive in childhood and became normotensive in adulthood did not have significant abnormalities in cardiac and renal functions. Thus prevention and control of hypertension in children and adolescents may have important beneficial effects on prevention of CVD in adulthood.</p>


Subject(s)
Adolescent , Adult , Blood Pressure , Physiology , Child , Cohort Studies , Female , Follow-Up Studies , Humans , Hypertension , Epidemiology , Kidney Function Tests , Male , Young Adult
19.
Chinese Journal of Epidemiology ; (12): 849-854, 2008.
Article in Chinese | WPRIM | ID: wpr-298367

ABSTRACT

Objective To explore the association between histories of hypertension in parents and level of blood pressure in their children so as to provide strategy for early intervention. Methods 19 088 children aged 6-17 years were selected in Beijing with stratified random cluster sampling method,and data on influencing factors including parental history of hypertension and other related environmental factors were collected.Systolic blood pressure (SBP) and diastolic blood pressure (DBP) were examined.SPSS 13.0 was used for data analyses.Results The average prevalence of high blood pressure in children and adolescents was 9.13%based on the blood pressure criteria Beijing Children and Adolescents Metabolic Syndrome (BCAMS) set in 2004.Familial aggregation of hypertension in children and adolescents was observed (P<0.001).There were positive associations between the numbers of parents with history of hypertension and both SBP and DBP of their offspring.Partial regression coefficients appeared to be 0.980 (95%CI:0.524-1.437) and 0.832 (95%CI:0.463-1.201) respectively,after controlling for con founding variables including gender,age,residential regions,body mass index (BMI),pubertal development,histories of smoking and drinking alcohol,fat intakes,physical exercises,parents' education level etc.Results from multiple factor logistic regression analysis showed that when compared with children whose parents did not have hypertension,the odds ratios of children having high blood pressure with only paternal history,only maternal history or with both parental histories were 1.688 (1.385-2.059),1.559 (1.164-2.087) and 1.273 (0.673-2.406),respectively,after adjustment for confounding factors.Conclusion Parental history of hypertension seemed to be an important independent risk factor for high blood pressure to their offspring.Heredity factors should be emphasized in the development of prevention and intervention on hypertension in children and adolescents.

20.
Chinese Journal of Epidemiology ; (12): 110-115, 2008.
Article in Chinese | WPRIM | ID: wpr-322818

ABSTRACT

<p><b>OBJECTIVE</b>The best approach for blood pressure (BP) measurement in children remains controversial, especially on the choice of Korotkoff phase 4 (K4) vs. Korotkoff phase 5 (K5) for diastolic BP (DBP) and to compare the differences between K4 and K5 in school-aged children and their predictions to hypertension in adult.</p><p><b>METHODS</b>The "Beijing children and adolescents BP study" cohort population consisted 2505 school-aged children aged 6 to 17 at baseline survey in 1987, when datum of systolic BP (SBP), DBP measured using K4 and K5, were collected respectively. Among them, 412 individuals with 220 males and 192 females at age of 23-37 years old, were successfully followed up and invited to take part in a clinical examination including anthropometric measurements, SBP and DBP recordings, and a questionnaires in 2005. Method for the BP measurements at both baseline and followed-up was by auscultation with a standard sphygmomanometer. Child hypertension at baseline was diagnosed according to the age-specific cutoffs recommended by the World Health Organization in 1996 (WHO 1996). Adult hypertension was diagnosed according to the China Guideline for Hypertension Prevention and Control issued in 2005. Partial correlation coefficients were calculated to describe the association of K4 and K5 in childhood with SBP and DBP level in adulthood. Multivariate logistic regression analysis was conducted to examine the impact of choice of K5 and K4 as DBP in childhood on prediction to the hypertension in adulthood. Potential confounders such as gender and adult-height were controlled for.</p><p><b>RESULTS</b>At baseline, the prevalence rates of hypertension were 5.0% with DBP measured using K4, and 2.4% with DBP measured using K5, respectively. The geometric mean difference value of K4 minus K5 (K4 - K5) was (10.1 +/- 1.7 mm Hg) (1 mm Hg = 0.133 kPa) for the 2505 school-aged children, and decreased as age increased. There was no significant difference regarding the values of K4 - K5 between males and females in all age groups except for children who at pubertal stage. The distribution of K4 - K5 value across age groups was statistically significant (P < 0.001), 59.6%, 60.5%, 56.3% and 45.1% of children who aged 6-9 years, 10-12 years, 13-15 years, and 16-17 years with their K4 - K5 value over 10 mm Hg. K4 in childhood was better correlated to both SBP and DBP in adulthood than K5. In general, K4 seemed to be superior to K5 in predicting hypertension in adulthood. The odds ratios of hypertension in adulthood were 1.69 (95% CI: 1.11-2.00), 1.45 (1.05-2.02), 2.18 (1.37-3.47), and 1.66 (1.07-2.59) with each 5 mmHg increasing of K4 measured in children aged 6-9 years, 10-12 years, 13-15 years, and 16-17 years, respectively. The agreement diagnosis between child hypertension and adult hypertension was higher for childhood DBP measured using K4 (20.2%) than that using K5 (12.8%).</p><p><b>CONCLUSION</b>There was significant difference between K4 and K5 in Chinese children and adolescents. Choice of diastolic Korotkoff blood pressure could affect DBP tracking from childhood into adulthood. K4 seemed superior to K5 when using auscultator technique to measure DBP.</p>


Subject(s)
Adolescent , Adult , Blood Pressure , Physiology , Child , Female , Humans , Hypertension , Epidemiology , Male
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