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Article in Chinese | WPRIM | ID: wpr-828496


OBJECTIVE@#To establish the optimum extraction technique and high performance liquid chromatographic (HPLC) method to simultaneously quantify nine compounds of gallic acid, hydroxy-paeoniflorin, catechin, albiflorin, paeoniflorin, pentagalloylglucose, benzoic acid, benzoylpaeoniflorin and paeonol in .@*METHODS@#Linear gradient elution was applied using water containing 0.1%phosphoric acid and acetonitrile as the mobile phase with a flow rate of 0.8 mL/min, column temperature of 30℃ and wavelength of 230 nm. The method of ultrasound extraction was used. Methanol and ethanol were used as extraction solvents, and three factors and three levels of orthogonal experiments was designed using L (3 ) table to investigate the effects of solvent concentration, ratio of liquid to material and extraction time on the total content of nine components of .@*RESULTS@#HPLC method was verified to have high specificity, sensitivity and accuracy through methodological validation, and it could be used for simultaneous quantitative analysis of nine components of . The results showed that the optimum extraction technology of nine components of was using 70%ethanol as extraction solvent, ratio of liquid to material was 200 mL/g and ultrasound extraction time was 30 min.@*CONCLUSIONS@#HPLC method for the simultaneous determination of nine components of is established, and the optimum extraction technology is confirmed.

Chromatography, High Pressure Liquid , Drugs, Chinese Herbal , Paeonia
Article in Chinese | WPRIM | ID: wpr-604845


Objective To analyze the changes of splenic volume and blood flow detected by ultrasonography in men having been migra-ted in high-altitude area for 10 years and to explore the relationship between the splenic blood flow and volume. Methods Men migrating to high-altitude area for 10 years,male indigenous Tibetan and healthy men from planitia with 55 cases in each were involved. Ultrasonography was performed to measure the length,width and thickness of spleen to calculate the volume,and color Doppler flow imaging was applied to measure the diameter of splenic artery and the mean flow velocity to calculate the blood flow volume. The differences among groups were com-pared and the relationship between the blood flow and the volume was analyzed. Results The blood flow and the volume of spleen in men migrating to high-altitude area were higher than those in male indigenous Tibetan and healthy men from planitia (P<0. 01),and the blood flow was positively correlated with the splenic volume (r=0. 828,P<0. 01). Conclusion The splenic volume and blood flow are increased in men when have been migrated in high-altitude area for 10 years and ultrasonography is of great importance in detecting the pathophysiologic changes in high-altitude area in our country.

Article in Chinese | WPRIM | ID: wpr-678168


Objective: To study the anti hyperglycemic effect of rubusoside on diabetic rats induced by streptozotocin (STZ)and its mechanism . Methods: The STZ induced diabetic rats were dived into three groups and were given (ig.) saline, rubusoside and Phenformin respectively once a day. After three weeks, the levels of serum glucose, fructosamine, insulin,SOD were assayed. Results: Rubusoside could reduce the level of serum glucose significantly, enhance the ability of anti oxidation and stimulate the secretion of insulin in STZ induced diabetic rats. Conclusion: The action of rubusoside on secretion of insulin can be explained for the anti hyperglycemic effect.